MD02G1194500.v1.1

Malate dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014980
Physical Location & Seq
Forward (+)
18555695 .. 18566704
11010 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1194500.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGCAGCAGCAGCCGAGCCCAGAAGTAAACGCGTATCAACGGATTGCTAGAATTTCCGCCCATTTGGACCCACCAAATCTCCAGATGGAGAACGCTGCCACTGTTAGCCGCTTGAATTGCCGCGCCAAGGGCGGCGCCGCTGGTTTCAAAGTAGCCATACTTGGAGCAGCCGGCGGCATAGGCCAGCCTCTCGCCTTGTTAATGAAGATGAACCCCTTGGTCTCCGTTATCCACCTATACGACGTCGTCAACACCCCCGGCGTCACCTCCGATATCAGCCACATGGACACCGGCGCCGTAGTGCGTGGGTTTTTGGGGCAGCAGCAGCTGGAGGAAGCGCTGACCGGAATGGAGCTGGTGATCATTCCGGCCGGTGTGCCCCGAAAACCCGGAATGACAAGGGACGATTTGTTCAACATCAATGCCGGAATCGTCAAGACGATCAGTGAAGCAATCGCCAAGTGCTGTCCGTATGCCATTGTTAACGTGATTAGCAACCCTGTGAACTCCACAGTTCCCATTGCTGCTGAAGTTTTCAAAAAAGCTGGTACCTTTGATCCTAAGAAACTATTGGGAGTCACCATGCTTGATGTTGTTAGAGCCAATACTTTTGTGGCAGAGGTTCTGGGTCTTGATCCTAGGGACATCGACGTCCCCGTTGTTGGGGGTCATGCAGGGGTTACAATTTTGCCTCTTCTGTCTCAGGTTAAACCTCCATGCTCTTTCACCCCAAAAGAAGTTGATTACCTAACAGATCGCATTCAGAATGGTGGCACTGAGGTGGTTGAGGCAAAAGCTGGAGCTGGTTCTGCAACATTATCAATGGCATATGCTGCTGTTAAATTTGCAGACGCATGCCTCCGTGGCTTGCGAGGAGATGCTGGGGTTGTCGAATGTGCATTTTTGGCTTCTCAGGTGACTGAACTTCCCTTCTTTGCATCCAAGGTGCGGCTTGGCCGTACTGGAGCCGAGGAAATCTACCCTCTTGGCCCCCTGAATGAGTATGAGAGGGCTGGCTTGGAGAAGGCAAAGAAAGAACTAGCAGCAAGCATTCAGAAGGGGGTTTCTTTCGTCAGAAAATGA

Protein Analysis

361

Amino Acids

38.02

Weight (kDa)

8.14

Isoelectric Point (pI)

28.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ldh_1_N PF00056 50 - 192 5e-46 lactate/malate dehydrogenase, NAD binding domain
Ldh_1_C PF02866 194 - 357 2.3e-44 lactate/malate dehydrogenase, alpha/beta C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014980)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G22780
fragaria_vesca FvH4_1g18690 FvH4_1g18690 FvH4_1g18690
malus_domestica MD02G1194500.v1.1 MD15G1304800.v1.1
prunus_persica Prupe.6G212400_v2.0.a1 Prupe.6G212400_v2.0.a1
pyrus_communis pycom02g15840 pycom15g26720
rosa_chinensis RchiOBHm_Chr2g0109561
rosa_laevigata RLG00000017745
rosa_multiflora Rmu_sc0000332.1_g000042
rosa_roxburghii Rroxscaffold_2G00134150
rosa_rugosa Rorug02G0160600
rosa_samantha Rh2BG223200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 650
AatII GACGTC 2 cut(s) 246, 654
Acc65I GGTACC 1 cut(s) 548
AccB1I GGYRCC 3 cut(s) 134, 293, 548
AccII CGCG 2 cut(s) 32, 123
AciI CCGC 7 cut(s) 57, 109, 121, 132, 138, 174, 949
AclWI GGATC 2 cut(s) 551, 629
AcoI YGGCCR 2 cut(s) 369, 955
AcsI RAATTY 2 cut(s) 51, 842
AcuI CTGAAG 1 cut(s) 549
AcyI GRCGYC 5 cut(s) 135, 243, 261, 294, 651
AfaI GTAC 2 cut(s) 550, 961
AfeI AGCGCT 1 cut(s) 339
AfiI CCNNNNNNNGG 4 cut(s) 127, 662, 663, 948
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 4 cut(s) 115, 148, 415, 538
AleI CACNNNNGTG 1 cut(s) 779
AloI GAACNNNNNNTCC 2 cut(s) 395, 427
AluBI AGCT 5 cut(s) 328, 355, 545, 797, 803
AluI AGCT 5 cut(s) 328, 355, 545, 797, 803
Alw26I GTCTC 2 cut(s) 226, 705
AlwI GGATC 2 cut(s) 551, 629
AlwNI CAGNNNCTG 1 cut(s) 328
Aor51HI AGCGCT 1 cut(s) 339
AoxI GGCC 4 cut(s) 181, 369, 955, 988
ApoI RAATTY 2 cut(s) 51, 842
Asp718I GGTACC 1 cut(s) 548
AspA2I CCTAGG 1 cut(s) 638
AspLEI GCGC 4 cut(s) 125, 137, 296, 340
AspS9I GGNCC 2 cut(s) 67, 989
AsuC2I CCSGG 2 cut(s) 258, 390
AsuHPI GGTGA 5 cut(s) 256, 370, 571, 718, 928
AvaII GGWCC 1 cut(s) 67
AvrII CCTAGG 1 cut(s) 638
BaeGI GKGCMC 1 cut(s) 381
BanI GGYRCC 3 cut(s) 134, 293, 548
BanII GRGCYC 1 cut(s) 20
BccI CCATC 1 cut(s) 79
BceAI ACGGC 2 cut(s) 281, 942
BclI TGATCA 1 cut(s) 360
BcnI CCSGG 2 cut(s) 258, 390
BcoDI GTCTC 2 cut(s) 226, 705
BfaI CTAG 3 cut(s) 48, 639, 1040
BfoI RGCGCY 3 cut(s) 138, 297, 341
BglI GCCNNNNNGGC 1 cut(s) 864
BlnI CCTAGG 1 cut(s) 638
Bme1390I CCNGG 2 cut(s) 258, 390
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 2 cut(s) 67, 989
BmiI GGNNCC 6 cut(s) 69, 136, 295, 550, 967, 991
BmrFI CCNGG 2 cut(s) 258, 390
BmsI GCATC 2 cut(s) 868, 947
BpmI CTGGAG 4 cut(s) 65, 350, 819, 984
BpuMI CCSGG 2 cut(s) 258, 390
BsaHI GRCGYC 5 cut(s) 135, 243, 261, 294, 651
BsaI GGTCTC 1 cut(s) 226
BsaJI CCNNGG 7 cut(s) 126, 216, 256, 638, 862, 942, 969
BsaWI WCCGGW 1 cut(s) 344
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 662, 663, 948
Bse118I RCCGGY 3 cut(s) 170, 290, 371
Bse1I ACTGG 1 cut(s) 967
Bse3DI GCAATG 1 cut(s) 519
BseDI CCNNGG 7 cut(s) 126, 216, 256, 638, 862, 942, 969
BseGI GGATG 1 cut(s) 938
BseLI CCNNNNNNNGG 4 cut(s) 127, 662, 663, 948
BseMI GCAATG 1 cut(s) 519
BseMII CTCAG 3 cut(s) 716, 768, 926
BseNI ACTGG 1 cut(s) 967
BseRI GAGGAG 1 cut(s) 888
BseSI GKGCMC 1 cut(s) 381
BseX3I CGGCCG 1 cut(s) 369
BseYI CCCAGC 1 cut(s) 881
Bsh1236I CGCG 2 cut(s) 32, 123
Bsh1285I CGRYCG 1 cut(s) 372
BshFI GGCC 4 cut(s) 183, 371, 957, 990
BshNI GGYRCC 3 cut(s) 134, 293, 548
BsiEI CGRYCG 1 cut(s) 372
BsiSI CCGG 8 cut(s) 171, 258, 291, 345, 368, 372, 390, 426
BslFI GGGAC 3 cut(s) 416, 638, 656
BslI CCNNNNNNNGG 4 cut(s) 127, 662, 663, 948
BsmAI GTCTC 2 cut(s) 226, 705
BsmFI GGGAC 3 cut(s) 416, 638, 656
BsmI GAATGC 2 cut(s) 759, 1050
BsnI GGCC 4 cut(s) 183, 371, 957, 990
Bso31I GGTCTC 1 cut(s) 226
Bsp1286I GDGCHC 2 cut(s) 20, 381
Bsp143I GATC 5 cut(s) 360, 441, 556, 634, 754
BspACI CCGC 7 cut(s) 57, 109, 121, 132, 138, 174, 949
BspANI GGCC 4 cut(s) 183, 371, 957, 990
BspCNI CTCAG 3 cut(s) 715, 769, 925
BspFNI CGCG 2 cut(s) 32, 123
BspLI GGNNCC 6 cut(s) 69, 136, 295, 550, 967, 991
BspPI GGATC 2 cut(s) 551, 629
BspT107I GGYRCC 3 cut(s) 134, 293, 548
BspTNI GGTCTC 1 cut(s) 226
BsrDI GCAATG 1 cut(s) 519
BsrFI RCCGGY 3 cut(s) 170, 290, 371
BsrI ACTGG 1 cut(s) 967
BssAI RCCGGY 3 cut(s) 170, 290, 371
BssECI CCNNGG 7 cut(s) 126, 216, 256, 638, 862, 942, 969
BssMI GATC 5 cut(s) 360, 441, 556, 634, 754
BssNI GRCGYC 5 cut(s) 135, 243, 261, 294, 651
BssT1I CCWWGG 4 cut(s) 126, 216, 638, 942
Bst4CI ACNGT 2 cut(s) 103, 514
Bst6I CTCTTC 1 cut(s) 699
BstACI GRCGYC 5 cut(s) 135, 243, 261, 294, 651
BstAPI GCANNNNNTGC 1 cut(s) 833
BstC8I GCNNGC 6 cut(s) 172, 185, 856, 869, 1015, 1048
BstDEI CTNAG 4 cut(s) 561, 702, 777, 912
BstDSI CCRYGG 1 cut(s) 862
BstF5I GGATG 1 cut(s) 938
BstFNI CGCG 2 cut(s) 32, 123
BstH2I RGCGCY 3 cut(s) 138, 297, 341
BstHHI GCGC 4 cut(s) 125, 137, 296, 340
BstKTI GATC 5 cut(s) 363, 444, 559, 637, 757
BstMAI GTCTC 2 cut(s) 226, 705
BstMBI GATC 5 cut(s) 360, 441, 556, 634, 754
BstMCI CGRYCG 1 cut(s) 372
BstMWI GCNNNNNNNGC 9 cut(s) 10, 117, 129, 180, 325, 809, 833, 864, 905
BstNSI RCATGY 1 cut(s) 858
BstSCI CCNGG 2 cut(s) 256, 388
BstSLI GKGCMC 1 cut(s) 381
BstUI CGCG 2 cut(s) 32, 123
BstZI CGGCCG 1 cut(s) 369
BsuRI GGCC 4 cut(s) 183, 371, 957, 990
BtgI CCRYGG 1 cut(s) 862
BtsCI GGATG 1 cut(s) 938
BtsIMutI CAGTG 3 cut(s) 99, 451, 774
Cac8I GCNNGC 6 cut(s) 172, 185, 856, 869, 1015, 1048
CaiI CAGNNNCTG 1 cut(s) 328
CfoI GCGC 4 cut(s) 125, 137, 296, 340
Cfr10I RCCGGY 3 cut(s) 170, 290, 371
Cfr13I GGNCC 2 cut(s) 67, 989
CseI GACGC 2 cut(s) 250, 860
Csp6I GTAC 2 cut(s) 549, 960
CviAII CATG 5 cut(s) 283, 583, 671, 717, 855
CviQI GTAC 2 cut(s) 549, 960
DdeI CTNAG 4 cut(s) 561, 702, 777, 912
DinI GGCGCC 2 cut(s) 136, 295
DpnI GATC 5 cut(s) 362, 443, 558, 636, 756
DpnII GATC 5 cut(s) 360, 441, 556, 634, 754
DrdI GACNNNNNNGTC 1 cut(s) 650
DseDI GACNNNNNNGTC 1 cut(s) 650
EaeI YGGCCR 2 cut(s) 369, 955
EagI CGGCCG 1 cut(s) 369
Eam1104I CTCTTC 1 cut(s) 699
EarI CTCTTC 1 cut(s) 699
EciI GGCGGA 1 cut(s) 46
EclXI CGGCCG 1 cut(s) 369
Eco130I CCWWGG 4 cut(s) 126, 216, 638, 942
Eco24I GRGCYC 1 cut(s) 20
Eco31I GGTCTC 1 cut(s) 226
Eco32I GATATC 1 cut(s) 274
Eco47I GGWCC 1 cut(s) 67
Eco47III AGCGCT 1 cut(s) 339
Eco52I CGGCCG 1 cut(s) 369
Eco57I CTGAAG 1 cut(s) 549
EcoRV GATATC 1 cut(s) 274
EcoT14I CCWWGG 4 cut(s) 126, 216, 638, 942
EcoT38I GRGCYC 1 cut(s) 20
EgeI GGCGCC 2 cut(s) 136, 295
EheI GGCGCC 2 cut(s) 136, 295
ErhI CCWWGG 4 cut(s) 126, 216, 638, 942
FaeI CATG 5 cut(s) 286, 586, 674, 720, 858
FaqI GGGAC 3 cut(s) 416, 638, 656
FatI CATG 5 cut(s) 282, 582, 670, 716, 854
FauNDI CATATG 1 cut(s) 829
FbaI TGATCA 1 cut(s) 360
FokI GGATG 1 cut(s) 925
FriOI GRGCYC 1 cut(s) 20
FspBI CTAG 3 cut(s) 48, 639, 1040
GlaI GCGC 4 cut(s) 124, 136, 295, 339
GsaI CCCAGC 1 cut(s) 885
GsuI CTGGAG 4 cut(s) 65, 350, 819, 984
HaeII RGCGCY 3 cut(s) 138, 297, 341
HaeIII GGCC 4 cut(s) 183, 371, 957, 990
HapII CCGG 8 cut(s) 171, 258, 291, 345, 368, 372, 390, 426
HgaI GACGC 2 cut(s) 250, 860
HhaI GCGC 4 cut(s) 125, 137, 296, 340
Hin1I GRCGYC 5 cut(s) 135, 243, 261, 294, 651
Hin1II CATG 5 cut(s) 286, 586, 674, 720, 858
Hin6I GCGC 4 cut(s) 123, 135, 294, 338
HinP1I GCGC 4 cut(s) 123, 135, 294, 338
HincII GTYRAC 2 cut(s) 250, 484
HindII GTYRAC 2 cut(s) 250, 484
HinfI GANTC 2 cut(s) 429, 576
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 8 cut(s) 171, 258, 291, 345, 368, 372, 390, 426
HphI GGTGA 5 cut(s) 256, 370, 571, 718, 928
Hpy166II GTNNAC 4 cut(s) 28, 250, 484, 505
Hpy188I TCNGA 4 cut(s) 271, 765, 1056, 1076
Hpy188III TCNNGA 3 cut(s) 82, 436, 632
Hpy8I GTNNAC 4 cut(s) 28, 250, 484, 505
Hpy99I CGWCG 3 cut(s) 245, 248, 653
HpyAV CCTTC 3 cut(s) 940, 1018, 1051
HpyCH4III ACNGT 2 cut(s) 103, 514
HpyCH4IV ACGT 3 cut(s) 243, 486, 651
HpyCH4V TGCA 6 cut(s) 4, 674, 812, 848, 899, 938
HpyF10VI GCNNNNNNNGC 9 cut(s) 10, 117, 129, 180, 325, 809, 833, 864, 905
HpyF3I CTNAG 4 cut(s) 561, 702, 777, 912
HpySE526I ACGT 3 cut(s) 243, 486, 651
Hsp92I GRCGYC 5 cut(s) 135, 243, 261, 294, 651
Hsp92II CATG 5 cut(s) 286, 586, 674, 720, 858
HspAI GCGC 4 cut(s) 123, 135, 294, 338
KasI GGCGCC 2 cut(s) 134, 293
KpnI GGTACC 1 cut(s) 552
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 360
KspAI GTTAAC 1 cut(s) 484
Kzo9I GATC 5 cut(s) 360, 441, 556, 634, 754
LmnI GCTCC 4 cut(s) 164, 352, 800, 965
LweI GCATC 2 cut(s) 868, 947
MaeI CTAG 3 cut(s) 48, 639, 1040
MaeII ACGT 3 cut(s) 243, 486, 651
MaeIII GTNAC 4 cut(s) 262, 577, 679, 916
MalI GATC 5 cut(s) 362, 443, 558, 636, 756
MboI GATC 5 cut(s) 360, 441, 556, 634, 754
MboII GAAGA 2 cut(s) 217, 686
MhlI GDGCHC 2 cut(s) 20, 381
MluCI AATT 4 cut(s) 51, 115, 684, 842
MluI ACGCGT 1 cut(s) 30
Mly113I GGCGCC 2 cut(s) 135, 294
MlyI GAGTC 1 cut(s) 585
MroNI GCCGGC 1 cut(s) 170
MseI TTAA 4 cut(s) 200, 483, 708, 840
MslI CAYNNNNRTG 1 cut(s) 779
MspA1I CMGCKG 2 cut(s) 140, 328
MspI CCGG 8 cut(s) 171, 258, 291, 345, 368, 372, 390, 426
MspR9I CCNGG 2 cut(s) 258, 390
Mva1269I GAATGC 2 cut(s) 759, 1050
MvnI CGCG 2 cut(s) 32, 123
MwoI GCNNNNNNNGC 9 cut(s) 10, 117, 129, 180, 325, 809, 833, 864, 905
NaeI GCCGGC 1 cut(s) 172
NarI GGCGCC 2 cut(s) 135, 294
NciI CCSGG 2 cut(s) 258, 390
NdeI CATATG 1 cut(s) 829
NdeII GATC 5 cut(s) 360, 441, 556, 634, 754
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 5 cut(s) 286, 586, 674, 720, 858
NlaIV GGNNCC 6 cut(s) 69, 136, 295, 550, 967, 991
NmeAIII GCCGAG 2 cut(s) 39, 994
NmuCI GTSAC 3 cut(s) 262, 577, 916
NspI RCATGY 1 cut(s) 858
OliI CACNNNNGTG 1 cut(s) 779
PaeI GCATGC 1 cut(s) 858
PcsI WCGNNNNNNNCGW 1 cut(s) 654
PctI GAATGC 2 cut(s) 759, 1050
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 1 cut(s) 429
PflFI GACNNNGTC 1 cut(s) 245
PleI GAGTC 1 cut(s) 584
PluTI GGCGCC 2 cut(s) 138, 297
PpsI GAGTC 1 cut(s) 584
PspFI CCCAGC 1 cut(s) 881
PspN4I GGNNCC 6 cut(s) 69, 136, 295, 550, 967, 991
PspPI GGNCC 2 cut(s) 67, 989
PstNI CAGNNNCTG 1 cut(s) 328
PsyI GACNNNGTC 1 cut(s) 245
PvuII CAGCTG 1 cut(s) 328
RsaI GTAC 2 cut(s) 550, 961
RsaNI GTAC 2 cut(s) 549, 960
RseI CAYNNNNRTG 1 cut(s) 779
SaqAI TTAA 4 cut(s) 200, 483, 708, 840
Sau3AI GATC 5 cut(s) 360, 441, 556, 634, 754
Sau96I GGNCC 2 cut(s) 67, 989
SchI GAGTC 1 cut(s) 585
ScrFI CCNGG 2 cut(s) 258, 390
SduI GDGCHC 2 cut(s) 20, 381
SfaNI GCATC 2 cut(s) 868, 947
SfoI GGCGCC 2 cut(s) 136, 295
SgrAI CRCCGGYG 1 cut(s) 290
SinI GGWCC 1 cut(s) 67
SmiMI CAYNNNNRTG 1 cut(s) 779
SphI GCATGC 1 cut(s) 858
Sse9I AATT 4 cut(s) 51, 115, 684, 842
SsiI CCGC 7 cut(s) 57, 109, 121, 132, 138, 174, 949
SspDI GGCGCC 2 cut(s) 134, 293
SspMI CTAG 3 cut(s) 48, 639, 1040
StyD4I CCNGG 2 cut(s) 256, 388
StyI CCWWGG 4 cut(s) 126, 216, 638, 942
TaaI ACNGT 2 cut(s) 103, 514
TaiI ACGT 3 cut(s) 246, 489, 654
TaqI TCGA 2 cut(s) 648, 891
TasI AATT 4 cut(s) 51, 115, 684, 842
TauI GCSGC 6 cut(s) 111, 123, 135, 140, 177, 952
TfiI GAWTC 1 cut(s) 429
Tru1I TTAA 4 cut(s) 200, 483, 708, 840
Tru9I TTAA 4 cut(s) 200, 483, 708, 840
TscAI CASTG 3 cut(s) 106, 451, 781
TseFI GTSAC 3 cut(s) 262, 577, 916
Tsp45I GTSAC 3 cut(s) 262, 577, 916
TspDTI ATGAA 2 cut(s) 218, 224
TspGWI ACGGA 4 cut(s) 55, 214, 459, 851
TspRI CASTG 3 cut(s) 106, 451, 781
Tth111I GACNNNGTC 1 cut(s) 245
VpaK11BI GGWCC 1 cut(s) 67
XapI RAATTY 2 cut(s) 51, 842
XceI RCATGY 1 cut(s) 858
XcmI CCANNNNNNNNNTGG 1 cut(s) 610
XmaJI CCTAGG 1 cut(s) 638
XspI CTAG 3 cut(s) 48, 639, 1040
ZraI GACGTC 2 cut(s) 244, 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.