AT2G23050

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0003992
Physical Location & Seq
Forward (+)
9810525 .. 9812662
2138 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G23050.2

Sequence Viewer

Length: 1446 bp
ATGAAGTTTATGAAACTTGGAACCAAACCTGATTCATTTCTGTCTAAAGGAGACAATGTTAGGTATGTAACAAATGAGTTAGAAACAGAGATCATCATCATCATCGGCAATGTCAAGTTCTATCTGCATAAGTTCCCATTGCTGTCGAAAAGCGGATTCTTGCAGAAACACATAGCAACATCAAAAAATGAAGAGGAGAAGAAGAACCAAATTGATGAGATTGACATATCAGAGATTCCTGGTGGTTCTGTTGCTTTTGAGATTTGTGTCAAGTTCTGTTACGGCATCACGGTTACTTTAAACGCTTACAATGTGGTTGCGGTTCGTTGCGCGGCTGAGTTCTTGGAGATGAATGAAACGTTTGAGAAAAGCAATCTCGTTTACAAGATTGATGTTTTCTTGAACTCAACCATCTTCCGTAGTTGGAAAGATTCTATCATTGTTCTTCAGACCACAAAAGATCTCTTGTCTGATGACTCAGAGGAGCTCGTGAAGCGTTGTCTCGGCTCTATTGCTTCTACTGCTTCGATAGACACCTCGAAAGTCAAATGGTCTTATACTTACAACAGGAAGAAGAAGCTTGAGAAAGTGAGAAAACCAGAAGATGGTGTTCCAAAAGACTGGTGGGTGGAAGATTTATGCGAGCTTCACATCGATTTGTATAAACAAGCCATCAAAGCAATCAAGAACCGAGGTAAAGTCCCGAGTAACGTTATTGGAGAAGCATTACATGCTTATGCAATAAGAAGAATAGCTGGTTTTAGCAAAGAGTCCATGCAACTCATTGATAGATCTTTGATTAATACAATCATAGAGCTTCTCCCGGATGAGAAAGGAAACATTTCCTCTAGCTTCTTGACCAAGCTACATCGAGCATCGATCTTTCTTGGATGTGAAGAAACCGTGAAAGAAAAGTTAAAGAAAAGAGTCAGCGAGCAGCTTGAAGAGACAACAGTGAATGATATCTTAATGTATGATTTGGATATGGTGCAGAGCCTAGTGAAGGAGTTTATGAACCGTGATCCGAAAACTCACTCGAAAGTATCCGTGGCAAAGCTCATTGATGGATACTTAGCTGAGAAATCAAGAGATCCTAATCTCCCTCTTCAGAACTTCCTCTCTCTCGCTGAGACACTCTCTAGCTTCCCAAGACACTCTCACGACGTGTTATATCGCGCAATTGACATGTTTCTAAAGGAACATTCGGGGATTAGCAAGAGCGAGAAGAAGAGAGTATGCGGGCTAATGGATTGTAGGAAACTATCCGCGGAAGCGTGTGAACATGCGGTACAGAACGAACGTTTGCCAATGCGGGTGATTGTGCAAGTTCTCTTCTTTGAACAGATTAGAGCCAATGGTTCATCAACAGGATATAGCACTCCTGAGCTTACCACTACTACTCTAAACACTGAGGATGATGAATGGGACCATGAGAAAGAGTTTTGA

Protein Analysis

481

Amino Acids

54.95

Weight (kDa)

6.46

Isoelectric Point (pI)

32.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 91 - 310 2e-71 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 605
AccII CGCG 3 cut(s) 332, 1176, 1268
AciI CCGC 8 cut(s) 153, 320, 332, 1239, 1266, 1268, 1286, 1312
AclI AACGTT 3 cut(s) 359, 711, 1300
AclWI GGATC 2 cut(s) 1016, 1085
AcuI CTGAAG 2 cut(s) 431, 1091
AdeI CACNNNGTG 1 cut(s) 1165
AfaI GTAC 1 cut(s) 1290
AfiI CCNNNNNNNGG 2 cut(s) 605, 1003
AflIII ACRYGT 2 cut(s) 1164, 1185
AgsI TTSAA 3 cut(s) 403, 944, 1340
AjiI CACGTC 1 cut(s) 1165
AjnI CCWGG 1 cut(s) 238
Alw21I GWGCWC 1 cut(s) 489
Alw26I GTCTC 4 cut(s) 45, 506, 941, 1124
AlwI GGATC 2 cut(s) 1016, 1085
Ama87I CYCGRG 1 cut(s) 703
ApeKI GCWGC 1 cut(s) 937
AseI ATTAAT 1 cut(s) 801
Asp700I GAANNNNTTC 1 cut(s) 841
AspLEI GCGC 2 cut(s) 332, 1178
AspS9I GGNCC 1 cut(s) 1426
AsuC2I CCSGG 1 cut(s) 824
AsuHPI GGTGA 1 cut(s) 1327
AvaI CYCGRG 1 cut(s) 703
AvaII GGWCC 1 cut(s) 1426
BanII GRGCYC 1 cut(s) 489
BauI CACGAG 1 cut(s) 488
Bbv12I GWGCWC 1 cut(s) 489
BbvI GCAGC 1 cut(s) 949
BccI CCATC 4 cut(s) 419, 599, 680, 1058
BceAI ACGGC 1 cut(s) 298
BciT130I CCWGG 1 cut(s) 240
BciVI GTATCC 2 cut(s) 1054, 1061
BcnI CCSGG 1 cut(s) 824
BcoDI GTCTC 4 cut(s) 45, 506, 941, 1124
BfaI CTAG 3 cut(s) 849, 998, 1140
BfuI GTATCC 2 cut(s) 1054, 1061
BglII AGATCT 2 cut(s) 460, 791
BisI GCNGC 2 cut(s) 333, 938
BlsI GCNGC 2 cut(s) 334, 939
Bme1390I CCNGG 2 cut(s) 240, 824
Bme18I GGWCC 1 cut(s) 1426
BmeT110I CYCGRG 1 cut(s) 703
BmgBI CACGTC 1 cut(s) 1165
BmgT120I GGNCC 1 cut(s) 1426
BmiI GGNNCC 2 cut(s) 22, 1427
BmrFI CCNGG 2 cut(s) 240, 824
BmsI GCATC 2 cut(s) 294, 884
BplI GAGNNNNNCTC 2 cut(s) 1121, 1153
Bpu10I CCTNAGC 1 cut(s) 1383
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 824
Bsa29I ATCGAT 2 cut(s) 654, 878
BsaBI GATNNNNATC 2 cut(s) 95, 1095
BsaJI CCNNGG 3 cut(s) 691, 1047, 1266
BsaXI ACNNNNNCTCC 2 cut(s) 42, 72
Bsc4I CCNNNNNNNGG 2 cut(s) 605, 1003
Bse1I ACTGG 1 cut(s) 626
Bse3DI GCAATG 2 cut(s) 115, 137
Bse8I GATNNNNATC 2 cut(s) 95, 1095
BseBI CCWGG 1 cut(s) 240
BseCI ATCGAT 2 cut(s) 654, 878
BseDI CCNNGG 3 cut(s) 691, 1047, 1266
BseGI GGATG 3 cut(s) 832, 896, 1420
BseJI GATNNNNATC 2 cut(s) 95, 1095
BseLI CCNNNNNNNGG 2 cut(s) 605, 1003
BseMI GCAATG 2 cut(s) 115, 137
BseMII CTCAG 6 cut(s) 327, 492, 1068, 1119, 1374, 1401
BseNI ACTGG 1 cut(s) 626
BseRI GAGGAG 2 cut(s) 209, 497
BseXI GCAGC 1 cut(s) 949
BsgI GTGCAG 1 cut(s) 1010
Bsh1236I CGCG 3 cut(s) 332, 1176, 1268
BshVI ATCGAT 2 cut(s) 654, 878
BsiHKAI GWGCWC 1 cut(s) 489
BsiHKCI CYCGRG 1 cut(s) 703
BsiSI CCGG 1 cut(s) 824
BslFI GGGAC 2 cut(s) 686, 1439
BslI CCNNNNNNNGG 2 cut(s) 605, 1003
BsmAI GTCTC 4 cut(s) 45, 506, 941, 1124
BsmFI GGGAC 2 cut(s) 686, 1439
BsoBI CYCGRG 1 cut(s) 703
Bsp1286I GDGCHC 1 cut(s) 489
Bsp143I GATC 6 cut(s) 90, 460, 791, 879, 1021, 1090
BspACI CCGC 8 cut(s) 153, 320, 332, 1239, 1266, 1268, 1286, 1312
BspCNI CTCAG 6 cut(s) 328, 491, 1069, 1120, 1375, 1402
BspDI ATCGAT 2 cut(s) 654, 878
BspFNI CGCG 3 cut(s) 332, 1176, 1268
BspLI GGNNCC 2 cut(s) 22, 1427
BspPI GGATC 2 cut(s) 1016, 1085
BsrDI GCAATG 2 cut(s) 115, 137
BsrI ACTGG 1 cut(s) 626
BssECI CCNNGG 3 cut(s) 691, 1047, 1266
BssMI GATC 6 cut(s) 90, 460, 791, 879, 1021, 1090
BssSI CACGAG 1 cut(s) 488
Bst2BI CACGAG 1 cut(s) 488
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 4 cut(s) 292, 904, 955, 1019
Bst6I CTCTTC 5 cut(s) 186, 939, 1110, 1223, 1337
BstAPI GCANNNNNTGC 1 cut(s) 731
BstC8I GCNNGC 3 cut(s) 644, 935, 1241
BstDEI CTNAG 7 cut(s) 336, 478, 1072, 1077, 1128, 1383, 1410
BstDSI CCRYGG 2 cut(s) 1047, 1266
BstENI CCTNNNNNAGG 1 cut(s) 1001
BstF5I GGATG 3 cut(s) 832, 896, 1420
BstFNI CGCG 3 cut(s) 332, 1176, 1268
BstHHI GCGC 2 cut(s) 332, 1178
BstKTI GATC 6 cut(s) 93, 463, 794, 882, 1024, 1093
BstMAI GTCTC 4 cut(s) 45, 506, 941, 1124
BstMBI GATC 6 cut(s) 90, 460, 791, 879, 1021, 1090
BstMWI GCNNNNNNNGC 4 cut(s) 493, 521, 677, 731
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 3 cut(s) 734, 1189, 1286
BstSCI CCNGG 2 cut(s) 238, 822
BstUI CGCG 3 cut(s) 332, 1176, 1268
BstV1I GCAGC 1 cut(s) 949
BstX2I RGATCY 3 cut(s) 460, 791, 1090
BstXI CCANNNNNNTGG 1 cut(s) 621
BstYI RGATCY 3 cut(s) 460, 791, 1090
Bsu15I ATCGAT 2 cut(s) 654, 878
BsuI GTATCC 2 cut(s) 1054, 1061
BsuTUI ATCGAT 2 cut(s) 654, 878
BtgI CCRYGG 2 cut(s) 1047, 1266
BtrI CACGTC 1 cut(s) 1165
BtsCI GGATG 3 cut(s) 832, 896, 1420
BtsIMutI CAGTG 2 cut(s) 960, 1407
Cac8I GCNNGC 3 cut(s) 644, 935, 1241
CfoI GCGC 2 cut(s) 332, 1178
Cfr13I GGNCC 1 cut(s) 1426
Cfr42I CCGCGG 1 cut(s) 1269
ClaI ATCGAT 2 cut(s) 654, 878
Csp6I GTAC 1 cut(s) 1289
CviAII CATG 5 cut(s) 731, 775, 1186, 1283, 1430
CviQI GTAC 1 cut(s) 1289
DdeI CTNAG 7 cut(s) 336, 478, 1072, 1077, 1128, 1383, 1410
DpnI GATC 6 cut(s) 92, 462, 793, 881, 1023, 1092
DpnII GATC 6 cut(s) 90, 460, 791, 879, 1021, 1090
DraI TTTAAA 1 cut(s) 300
DraIII CACNNNGTG 1 cut(s) 1165
Eam1104I CTCTTC 5 cut(s) 186, 939, 1110, 1223, 1337
EarI CTCTTC 5 cut(s) 186, 939, 1110, 1223, 1337
Ecl136II GAGCTC 1 cut(s) 487
Eco24I GRGCYC 1 cut(s) 489
Eco32I GATATC 1 cut(s) 964
Eco47I GGWCC 1 cut(s) 1426
Eco53kI GAGCTC 1 cut(s) 487
Eco57I CTGAAG 2 cut(s) 431, 1091
Eco88I CYCGRG 1 cut(s) 703
EcoICRI GAGCTC 1 cut(s) 487
EcoNI CCTNNNNNAGG 1 cut(s) 1001
EcoRII CCWGG 1 cut(s) 238
EcoRV GATATC 1 cut(s) 964
EcoT38I GRGCYC 1 cut(s) 489
FaeI CATG 5 cut(s) 734, 778, 1189, 1286, 1433
FaqI GGGAC 2 cut(s) 686, 1439
FatI CATG 5 cut(s) 730, 774, 1185, 1282, 1429
FauI CCCGC 2 cut(s) 1232, 1305
Fnu4HI GCNGC 2 cut(s) 333, 938
FokI GGATG 3 cut(s) 839, 903, 1427
FriOI GRGCYC 1 cut(s) 489
Fsp4HI GCNGC 2 cut(s) 333, 938
FspBI CTAG 3 cut(s) 849, 998, 1140
GlaI GCGC 2 cut(s) 331, 1177
GluI GCNGC 2 cut(s) 333, 938
HapII CCGG 1 cut(s) 824
HhaI GCGC 2 cut(s) 332, 1178
Hin1II CATG 5 cut(s) 734, 778, 1189, 1286, 1433
Hin6I GCGC 2 cut(s) 330, 1176
HinP1I GCGC 2 cut(s) 330, 1176
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 7 cut(s) 32, 156, 235, 431, 476, 770, 927
HpaII CCGG 1 cut(s) 824
HphI GGTGA 1 cut(s) 1327
Hpy166II GTNNAC 2 cut(s) 382, 1280
Hpy188I TCNGA 6 cut(s) 232, 450, 472, 481, 1026, 1110
Hpy188III TCNNGA 8 cut(s) 400, 490, 685, 703, 856, 1086, 1160, 1382
Hpy8I GTNNAC 2 cut(s) 382, 1280
Hpy99I CGWCG 1 cut(s) 1166
HpyAV CCTTC 1 cut(s) 997
HpyCH4III ACNGT 4 cut(s) 292, 904, 955, 1019
HpyCH4IV ACGT 4 cut(s) 359, 711, 1164, 1300
HpyCH4V TGCA 6 cut(s) 127, 163, 740, 778, 991, 1324
HpyF10VI GCNNNNNNNGC 4 cut(s) 493, 521, 677, 731
HpyF3I CTNAG 7 cut(s) 336, 478, 1072, 1077, 1128, 1383, 1410
HpySE526I ACGT 4 cut(s) 359, 711, 1164, 1300
Hsp92II CATG 5 cut(s) 734, 778, 1189, 1286, 1433
HspAI GCGC 2 cut(s) 330, 1176
KspI CCGCGG 1 cut(s) 1269
Kzo9I GATC 6 cut(s) 90, 460, 791, 879, 1021, 1090
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 1 cut(s) 949
LweI GCATC 2 cut(s) 294, 884
MaeI CTAG 3 cut(s) 849, 998, 1140
MaeII ACGT 4 cut(s) 359, 711, 1164, 1300
MaeIII GTNAC 4 cut(s) 67, 278, 292, 707
MalI GATC 6 cut(s) 92, 462, 793, 881, 1023, 1092
MboI GATC 6 cut(s) 90, 460, 791, 879, 1021, 1090
MfeI CAATTG 1 cut(s) 1179
MflI RGATCY 3 cut(s) 460, 791, 1090
MhlI GDGCHC 1 cut(s) 489
MluCI AATT 2 cut(s) 210, 1179
MlyI GAGTC 3 cut(s) 470, 779, 936
MmeI TCCRAC 1 cut(s) 404
MnlI CCTC 8 cut(s) 187, 475, 547, 686, 856, 1113, 1127, 1405
MroXI GAANNNNTTC 1 cut(s) 841
MseI TTAA 4 cut(s) 299, 801, 917, 968
MslI CAYNNNNRTG 1 cut(s) 735
MspA1I CMGCKG 1 cut(s) 1268
MspI CCGG 1 cut(s) 824
MspR9I CCNGG 2 cut(s) 240, 824
MunI CAATTG 1 cut(s) 1179
MvaI CCWGG 1 cut(s) 240
MvnI CGCG 3 cut(s) 332, 1176, 1268
MwoI GCNNNNNNNGC 4 cut(s) 493, 521, 677, 731
NciI CCSGG 1 cut(s) 824
NdeII GATC 6 cut(s) 90, 460, 791, 879, 1021, 1090
NlaIII CATG 5 cut(s) 734, 778, 1189, 1286, 1433
NlaIV GGNNCC 2 cut(s) 22, 1427
NmeAIII GCCGAG 1 cut(s) 483
NspI RCATGY 3 cut(s) 734, 1189, 1286
PciI ACATGT 1 cut(s) 1185
PdmI GAANNNNTTC 1 cut(s) 841
PfeI GAWTC 4 cut(s) 32, 156, 235, 431
PflMI CCANNNNNTGG 1 cut(s) 605
PfoI TCCNGGA 1 cut(s) 822
PkrI GCNGC 2 cut(s) 334, 939
PleI GAGTC 3 cut(s) 470, 778, 935
PpsI GAGTC 3 cut(s) 470, 778, 935
PscI ACATGT 1 cut(s) 1185
PshBI ATTAAT 1 cut(s) 801
Psp124BI GAGCTC 1 cut(s) 489
Psp1406I AACGTT 3 cut(s) 359, 711, 1300
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 2 cut(s) 22, 1427
PspPI GGNCC 1 cut(s) 1426
PsrI GAACNNNNNNTAC 2 cut(s) 1272, 1304
PsuI RGATCY 3 cut(s) 460, 791, 1090
RsaI GTAC 1 cut(s) 1290
RsaNI GTAC 1 cut(s) 1289
RseI CAYNNNNRTG 1 cut(s) 735
SacI GAGCTC 1 cut(s) 489
SacII CCGCGG 1 cut(s) 1269
SaqAI TTAA 4 cut(s) 299, 801, 917, 968
SatI GCNGC 2 cut(s) 333, 938
Sau3AI GATC 6 cut(s) 90, 460, 791, 879, 1021, 1090
Sau96I GGNCC 1 cut(s) 1426
SchI GAGTC 3 cut(s) 470, 779, 936
ScrFI CCNGG 2 cut(s) 240, 824
SduI GDGCHC 1 cut(s) 489
SfaNI GCATC 2 cut(s) 294, 884
Sfr303I CCGCGG 1 cut(s) 1269
SgrBI CCGCGG 1 cut(s) 1269
SinI GGWCC 1 cut(s) 1426
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 2 cut(s) 210, 1179
SsiI CCGC 8 cut(s) 153, 320, 332, 1239, 1266, 1268, 1286, 1312
SspMI CTAG 3 cut(s) 849, 998, 1140
SstI GAGCTC 1 cut(s) 489
StyD4I CCNGG 2 cut(s) 238, 822
TaaI ACNGT 4 cut(s) 292, 904, 955, 1019
TaiI ACGT 4 cut(s) 362, 714, 1167, 1303
TaqI TCGA 7 cut(s) 146, 527, 539, 654, 871, 878, 1037
TasI AATT 2 cut(s) 210, 1179
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 4 cut(s) 32, 156, 235, 431
Tru1I TTAA 4 cut(s) 299, 801, 917, 968
Tru9I TTAA 4 cut(s) 299, 801, 917, 968
TscAI CASTG 2 cut(s) 960, 1414
TseI GCWGC 1 cut(s) 937
TspDTI ATGAA 9 cut(s) 17, 24, 26, 204, 365, 369, 1028, 1350, 1434
TspGWI ACGGA 2 cut(s) 407, 1036
TspRI CASTG 2 cut(s) 960, 1414
Van91I CCANNNNNTGG 1 cut(s) 605
VpaK11BI GGWCC 1 cut(s) 1426
VspI ATTAAT 1 cut(s) 801
XagI CCTNNNNNAGG 1 cut(s) 1001
XceI RCATGY 3 cut(s) 734, 1189, 1286
XcmI CCANNNNNNNNNTGG 1 cut(s) 621
XmnI GAANNNNTTC 1 cut(s) 841
XspI CTAG 3 cut(s) 849, 998, 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.