Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0003992

Physical Location & Seq

Genomic Coordinates Reverse (-)

16658430 .. 16663298

Gene Structure 4869 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1183500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1884 bp

ATGAAGTTCATGAAACTTGGATCGAAACCGGATTTCTTTCAGACCGACGGAGACAATATAAGATATGTCGCAACTGAGTTAGCAACTGATGTAGCTGTTAATGTCGGAGATGTGAAGTTTTATTTGCACAAGTTTCCTCTTTTGTCCAAGAGTGCACGCCTGCAGAAACTGGTCGCAACCACAAATGAAAACAGCGATGAAATCCACATCCATGATATTCCTGGCGGACCTGCTGCTTTTGAAATATGTGCCAAGTTCTGTTACGGCATGACTGTCACTCTCAATGCATACAATGTGGTTGCAACTCGATGCGCAGCAGAGTACCTGGAGATGTACGAAACTATTGATAAAGGAAACCTAGTCTACAAGGTTGAAGTGTTCCTAAACTCTAGCATCTTCCGGAGCTGGAAAGACTCGATTATTGTTCTTCAGACTACGAAGTCTCTTCTTCCATGGTCTGAGGAAGTTAAGGTGGTGAGCCACTGCCTCGATTCCATAGCTTCCAAGGCTACCATTGATACTTCCAAGGTGGAGTGGTCATATACATATAACCACAAAAAGCTACCGTCTAATAATGGGGATGATTCACACTGGAATGGTGTGGCAAAACAACAGATGGTTCCGAAAGACTGGTGGGTGGAAGACCTCTGTGGGCTTCAACTTGATTTATACAAGCGGGTGATAACAATAATTAAGACCAAAGGAAGCATTTCTGGTGATGTAATAGGGGAAGCCTTAAAGGCATATACTACCAGAAGGTTGTCAGGTTTTAGCAAGGGTATGATTCAGGGGGATGTCGTAAAGAATAAATCATTGGTGGAGACTGTAATCCACTTACTTCCTGCAGGCAGTGTCCCTTGCAGTTTCTTACTTAAATTGTTAAAAGCGGCAAGACTGTTGGAATGTGGAGAAGTCGAAAAATGCGAGTTAATTCGCAAAATTGGCCAGCAGCTTGACGAGGCTACAGTTTCTGATCTTTTGATTCAAGCCCCAACTGATGACGCAATAAAATATGAAGTTGATATAATGCAGAGCTTAATTGAAGAGTTTGTGACACACGATCGAAGTGCTCAGACTGATCCTTCAATGGAAAGTGAATTCCAGGAGATTAGAAGTCCCAGGTTATCGGATACTTCCAAGGTGAAGGTGGCAAAGCTGGTTGACAGCTATCTTACTGAGATTTCACGTGATCCCAACTTACCCGCGTCAAAGTTTGTCAATCTTGCTGAAATGGTATCAAGCTTCTCGAGACCAACTCATGATGGTCTTTACCGCGCCATTGACATGTATCTAAAGGAACACCCGGGGATCAGCAAGGGTGAGAGGAAGAGAATGTGCAAATTAATGGACTGCAGGAAGTTGTCAGTAGATGCCTGCATGCACGCTGTGCAAAATGAGCGGCTGCCCTTAAGAGTTGTTGTGCAGGTCCTTTTCTTTGAACAGATTAGGGCTTCAGCATCCGGTGGCAACAGTACACCTGATCTACAAGGCTCCATCAGGGCCTTGCTCCCTGGTGGGTCTCATGGGAGCTCAAGGTCTACAACCAACACAGCAGACGACTGGGATGCTGTGCCGACAGCAGAAGAAATCAAGGGTTTAAAATGTGAGATTTCTACTCTAAGGTTAGGCGAAGTGAATGGAGGAGTTGGAGTTGGAAGCGATAGAAATGGGAACCCCGGTGCTACAAAAAATCTTGAAAAAGTTCCATCTAGTAAAGTGAAAGGTATGTTCATGTCGAAAAAGATATTTTCTAAGCTGTGGTCAAGCAAAGAAAGGAGTGGTGAAATCAGCAGTTCTAATACATCAGAGAGCCCTGGGTCTTCTAGCAATGCAGAAGAAACAAAATCCACCTGTTCGAGGAGTAGGAGGAATTCGGTGTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005623 GO:0007275 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009791 GO:0009908 GO:0009958 GO:0022414 GO:0032501 GO:0032502 GO:0044464 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071944 GO:0090567

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

628

Amino Acids

69.29

Weight (kDa)

7.84

Isoelectric Point (pI)

39.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	27 - 117	3e-07	BTB/POZ domain
NPH3	PF03000	210 - 459	3.2e-85	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23050 AT2G23050 AT4G37590 AT5G67440 AT5G67440 AT5G67440
fragaria_vesca	FvH4_1g17440 FvH4_1g17440 FvH4_1g17440 FvH4_1g17440 FvH4_1g17440 FvH4_1g17440 FvH4_1g17440
malus_domestica	MD02G1183500.v1.1 MD15G1295200.v1.1
prunus_persica	Prupe.6G222500_v2.0.a1 Prupe.6G222500_v2.0.a1 Prupe.6G222500_v2.0.a1 Prupe.6G222500_v2.0.a1 Prupe.6G222500_v2.0.a1
pyrus_communis	pycom02g14920 pycom15g25820
rosa_chinensis	RchiOBHm_Chr2g0107651
rosa_laevigata	RLG00000017605
rosa_roxburghii	Rroxscaffold_2G00136090
rosa_rugosa	Rorug02G0146100 Rorug02G0146100
rosa_samantha	Rh2AG195900 Rh2BG208300 Rh2CG201000 Rh2DG203400 Rh6AG399700
rosa_wichuraiana	Rw2G015500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	439
Acc16I	TGCGCA	1 cut(s)	313
Acc36I	ACCTGC	2 cut(s)	238, 1414
AccBSI	CCGCTC	1 cut(s)	1399
AccI	GTMKAC	2 cut(s)	363, 1538
AccII	CGCG	2 cut(s)	1205, 1275
AccIII	TCCGGA	1 cut(s)	399
AciI	CCGC	6 cut(s)	225, 676, 887, 1203, 1273, 1399
AclWI	GGATC	4 cut(s)	28, 1073, 1184, 1316
AcoI	YGGCCR	1 cut(s)	943
AcsI	RAATTY	2 cut(s)	1097, 1870
AcuI	CTGAAG	2 cut(s)	413, 1437
AcvI	CACGTG	1 cut(s)	1187
AdeI	CACNNNGTG	1 cut(s)	1387
AfaI	GTAC	3 cut(s)	323, 335, 1474
AfiI	CCNNNNNNNGG	1 cut(s)	1857
AflII	CTTAAG	1 cut(s)	1408
AflIII	ACRYGT	1 cut(s)	1284
AgsI	TTSAA	8 cut(s)	242, 374, 659, 986, 1043, 1086, 1439, 1697
AjnI	CCWGG	6 cut(s)	220, 324, 1101, 1118, 1510, 1813
AjuI	GAANNNNNNNTTGG	2 cut(s)	797, 829
Alw21I	GWGCWC	3 cut(s)	157, 1072, 1532
Alw26I	GTCTC	5 cut(s)	45, 447, 815, 1243, 1524
Alw44I	GTGCAC	1 cut(s)	153
AlwI	GGATC	4 cut(s)	28, 1073, 1184, 1316
AlwNI	CAGNNNCTG	2 cut(s)	169, 971
Ama87I	CYCGRG	2 cut(s)	1246, 1303
Aor13HI	TCCGGA	1 cut(s)	399
AoxI	GGCC	2 cut(s)	943, 1500
ApaLI	GTGCAC	1 cut(s)	153
ApeKI	GCWGC	4 cut(s)	233, 314, 949, 1402
ApoI	RAATTY	2 cut(s)	1097, 1870
AseI	ATTAAT	1 cut(s)	1343
Asp700I	GAANNNNTTC	2 cut(s)	709, 1701
AspLEI	GCGC	2 cut(s)	314, 1277
AspS9I	GGNCC	3 cut(s)	227, 1426, 1500
AsuC2I	CCSGG	3 cut(s)	1304, 1305, 1677
AsuHPI	GGTGA	6 cut(s)	487, 691, 728, 1153, 1331, 1793
AvaI	CYCGRG	2 cut(s)	1246, 1303
AvaII	GGWCC	2 cut(s)	227, 1426
BaeGI	GKGCMC	1 cut(s)	157
BalI	TGGCCA	1 cut(s)	945
BanII	GRGCYC	2 cut(s)	1532, 1814
BbrPI	CACGTG	1 cut(s)	1187
BbsI	GAAGAC	2 cut(s)	648, 1812
Bbv12I	GWGCWC	3 cut(s)	157, 1072, 1532
BbvI	GCAGC	4 cut(s)	220, 326, 961, 1389
BccI	CCATC	4 cut(s)	610, 1256, 1502, 1714
BceAI	ACGGC	1 cut(s)	280
BcgI	CGANNNNNNTGC	4 cut(s)	1049, 1083, 1547, 1581
BciT130I	CCWGG	6 cut(s)	222, 326, 1103, 1120, 1512, 1815
BciVI	GTATCC	1 cut(s)	1123
BcnI	CCSGG	3 cut(s)	1304, 1305, 1677
BcoDI	GTCTC	5 cut(s)	45, 447, 815, 1243, 1524
BfaI	CTAG	4 cut(s)	359, 390, 1710, 1824
BfmI	CTRYAG	4 cut(s)	161, 843, 963, 1351
BfrI	CTTAAG	1 cut(s)	1408
BfuAI	ACCTGC	2 cut(s)	238, 1414
BfuI	GTATCC	1 cut(s)	1123
BglI	GCCNNNNNGGC	1 cut(s)	740
BisI	GCNGC	6 cut(s)	234, 315, 888, 950, 1400, 1403
BlsI	GCNGC	6 cut(s)	235, 316, 889, 951, 1401, 1404
Bme1390I	CCNGG	9 cut(s)	222, 326, 1103, 1120, 1304, 1305, 1512, 1677, 1815
Bme18I	GGWCC	2 cut(s)	227, 1426
BmeT110I	CYCGRG	2 cut(s)	1246, 1303
BmgT120I	GGNCC	3 cut(s)	227, 1426, 1500
BmiI	GGNNCC	3 cut(s)	621, 1492, 1673
BmrFI	CCNGG	9 cut(s)	222, 326, 1103, 1120, 1304, 1305, 1512, 1677, 1815
BmrI	ACTGGG	1 cut(s)	1570
BmsI	GCATC	5 cut(s)	299, 402, 1360, 1466, 1555
BmuI	ACTGGG	1 cut(s)	1570
BpiI	GAAGAC	2 cut(s)	648, 1812
BplI	GAGNNNNNCTC	2 cut(s)	1240, 1272
BpmI	CTGGAG	1 cut(s)	347
BpuEI	CTTGAG	1 cut(s)	1516
BpuMI	CCSGG	3 cut(s)	1304, 1305, 1677
BsaAI	YACGTR	1 cut(s)	1187
BsaI	GGTCTC	2 cut(s)	1243, 1524
BsaWI	WCCGGW	3 cut(s)	28, 399, 1460
BsaXI	ACNNNNNCTCC	2 cut(s)	1635, 1665
Bsc4I	CCNNNNNNNGG	1 cut(s)	1857
Bse1I	ACTGG	4 cut(s)	174, 596, 635, 1565
Bse3DI	GCAATG	1 cut(s)	1834
BseAI	TCCGGA	1 cut(s)	399
BseBI	CCWGG	6 cut(s)	222, 326, 1103, 1120, 1512, 1815
BseGI	GGATG	5 cut(s)	207, 586, 799, 1457, 1570
BseLI	CCNNNNNNNGG	1 cut(s)	1857
BseMI	GCAATG	1 cut(s)	1834
BseMII	CTCAG	4 cut(s)	66, 450, 1085, 1167
BseNI	ACTGG	4 cut(s)	174, 596, 635, 1565
BseRI	GAGGAG	2 cut(s)	1656, 1873
BseSI	GKGCMC	1 cut(s)	157
BseXI	GCAGC	4 cut(s)	220, 326, 961, 1389
BsgI	GTGCAG	1 cut(s)	1442
Bsh1236I	CGCG	2 cut(s)	1205, 1275
Bsh1285I	CGRYCG	1 cut(s)	1063
BshFI	GGCC	2 cut(s)	945, 1502
BsiEI	CGRYCG	1 cut(s)	1063
BsiHKAI	GWGCWC	3 cut(s)	157, 1072, 1532
BsiHKCI	CYCGRG	2 cut(s)	1246, 1303
BsiSI	CCGG	5 cut(s)	29, 400, 1304, 1461, 1677
BslFI	GGGAC	2 cut(s)	839, 1101
BslI	CCNNNNNNNGG	1 cut(s)	1857
BsmAI	GTCTC	5 cut(s)	45, 447, 815, 1243, 1524
BsmFI	GGGAC	2 cut(s)	839, 1101
BsnI	GGCC	2 cut(s)	945, 1502
Bso31I	GGTCTC	2 cut(s)	1243, 1524
BsoBI	CYCGRG	2 cut(s)	1246, 1303
Bsp1286I	GDGCHC	4 cut(s)	157, 1072, 1532, 1814
Bsp13I	TCCGGA	1 cut(s)	399
Bsp143I	GATC	7 cut(s)	20, 973, 1060, 1078, 1189, 1308, 1480
Bsp19I	CCATGG	1 cut(s)	452
BspACI	CCGC	6 cut(s)	225, 676, 887, 1203, 1273, 1399
BspANI	GGCC	2 cut(s)	945, 1502
BspCNI	CTCAG	4 cut(s)	67, 451, 1084, 1168
BspEI	TCCGGA	1 cut(s)	399
BspFNI	CGCG	2 cut(s)	1205, 1275
BspHI	TCATGA	2 cut(s)	9, 1258
BspLI	GGNNCC	3 cut(s)	621, 1492, 1673
BspMAI	CTGCAG	3 cut(s)	165, 847, 1355
BspMI	ACCTGC	2 cut(s)	238, 1414
BspPI	GGATC	4 cut(s)	28, 1073, 1184, 1316
BspTI	CTTAAG	1 cut(s)	1408
BspTNI	GGTCTC	2 cut(s)	1243, 1524
BsrBI	CCGCTC	1 cut(s)	1399
BsrDI	GCAATG	1 cut(s)	1834
BsrI	ACTGG	4 cut(s)	174, 596, 635, 1565
BssMI	GATC	7 cut(s)	20, 973, 1060, 1078, 1189, 1308, 1480
BssT1I	CCWWGG	4 cut(s)	452, 504, 525, 1137
Bst2UI	CCWGG	6 cut(s)	222, 326, 1103, 1120, 1512, 1815
Bst4CI	ACNGT	6 cut(s)	274, 567, 826, 897, 967, 1472
Bst6I	CTCTTC	3 cut(s)	450, 1038, 1322
BstAFI	CTTAAG	1 cut(s)	1408
BstAPI	GCANNNNNTGC	1 cut(s)	1387
BstBAI	YACGTR	1 cut(s)	1187
BstC8I	GCNNGC	7 cut(s)	157, 161, 847, 947, 1375, 1379, 1383
BstDEI	CTNAG	7 cut(s)	75, 459, 1071, 1176, 1619, 1752, 1881
BstDSI	CCRYGG	1 cut(s)	452
BstENI	CCTNNNNNAGG	1 cut(s)	1855
BstF5I	GGATG	5 cut(s)	207, 586, 799, 1457, 1570
BstFNI	CGCG	2 cut(s)	1205, 1275
BstHHI	GCGC	2 cut(s)	314, 1277
BstKTI	GATC	7 cut(s)	23, 976, 1063, 1081, 1192, 1311, 1483
BstMAI	GTCTC	5 cut(s)	45, 447, 815, 1243, 1524
BstMBI	GATC	7 cut(s)	20, 973, 1060, 1078, 1189, 1308, 1480
BstMCI	CGRYCG	1 cut(s)	1063
BstMWI	GCNNNNNNNGC	5 cut(s)	506, 740, 942, 1387, 1396
BstNI	CCWGG	6 cut(s)	222, 326, 1103, 1120, 1512, 1815
BstNSI	RCATGY	2 cut(s)	1288, 1381
BstSCI	CCNGG	9 cut(s)	220, 324, 1101, 1118, 1302, 1303, 1510, 1675, 1813
BstSFI	CTRYAG	4 cut(s)	161, 843, 963, 1351
BstSLI	GKGCMC	1 cut(s)	157
BstUI	CGCG	2 cut(s)	1205, 1275
BstV1I	GCAGC	4 cut(s)	220, 326, 961, 1389
BstV2I	GAAGAC	2 cut(s)	648, 1812
BsuI	GTATCC	1 cut(s)	1123
BsuRI	GGCC	2 cut(s)	945, 1502
BtgI	CCRYGG	1 cut(s)	452
BtgZI	GCGATG	1 cut(s)	210
BtsCI	GGATG	5 cut(s)	207, 586, 799, 1457, 1570
BtsI	GCAGTG	2 cut(s)	481, 856
BtsIMutI	CAGTG	3 cut(s)	481, 589, 856
BveI	ACCTGC	2 cut(s)	238, 1414
Cac8I	GCNNGC	7 cut(s)	157, 161, 847, 947, 1375, 1379, 1383
CaiI	CAGNNNCTG	2 cut(s)	169, 971
CciI	TCATGA	2 cut(s)	9, 1258
CfoI	GCGC	2 cut(s)	314, 1277
Cfr13I	GGNCC	3 cut(s)	227, 1426, 1500
Cfr9I	CCCGGG	1 cut(s)	1303
CseI	GACGC	2 cut(s)	1010, 1194
Csp6I	GTAC	3 cut(s)	322, 334, 1473
CviAII	CATG	9 cut(s)	10, 212, 268, 453, 1259, 1285, 1378, 1523, 1732
CviQI	GTAC	3 cut(s)	322, 334, 1473
DdeI	CTNAG	7 cut(s)	75, 459, 1071, 1176, 1619, 1752, 1881
DpnI	GATC	7 cut(s)	22, 975, 1062, 1080, 1191, 1310, 1482
DpnII	GATC	7 cut(s)	20, 973, 1060, 1078, 1189, 1308, 1480
DraI	TTTAAA	1 cut(s)	1599
DraIII	CACNNNGTG	1 cut(s)	1387
DrdI	GACNNNNNNGTC	1 cut(s)	439
DseDI	GACNNNNNNGTC	1 cut(s)	439
EaeI	YGGCCR	1 cut(s)	943
Eam1104I	CTCTTC	3 cut(s)	450, 1038, 1322
EarI	CTCTTC	3 cut(s)	450, 1038, 1322
EciI	GGCGGA	1 cut(s)	240
Ecl136II	GAGCTC	1 cut(s)	1530
Eco130I	CCWWGG	4 cut(s)	452, 504, 525, 1137
Eco24I	GRGCYC	2 cut(s)	1532, 1814
Eco31I	GGTCTC	2 cut(s)	1243, 1524
Eco47I	GGWCC	2 cut(s)	227, 1426
Eco53kI	GAGCTC	1 cut(s)	1530
Eco57I	CTGAAG	2 cut(s)	413, 1437
Eco72I	CACGTG	1 cut(s)	1187
Eco88I	CYCGRG	2 cut(s)	1246, 1303
EcoICRI	GAGCTC	1 cut(s)	1530
EcoNI	CCTNNNNNAGG	1 cut(s)	1855
EcoO109I	RGGNCCY	2 cut(s)	1426, 1500
EcoRI	GAATTC	2 cut(s)	1097, 1870
EcoRII	CCWGG	6 cut(s)	220, 324, 1101, 1118, 1510, 1813
EcoT14I	CCWWGG	4 cut(s)	452, 504, 525, 1137
EcoT22I	ATGCAT	1 cut(s)	289
EcoT38I	GRGCYC	2 cut(s)	1532, 1814
ErhI	CCWWGG	4 cut(s)	452, 504, 525, 1137
FaeI	CATG	9 cut(s)	13, 215, 271, 456, 1262, 1288, 1381, 1526, 1735
FaqI	GGGAC	2 cut(s)	839, 1101
FatI	CATG	9 cut(s)	9, 211, 267, 452, 1258, 1284, 1377, 1522, 1731
FauI	CCCGC	2 cut(s)	669, 1210
FblI	GTMKAC	2 cut(s)	363, 1538
Fnu4HI	GCNGC	6 cut(s)	234, 315, 888, 950, 1400, 1403
FokI	GGATG	5 cut(s)	194, 593, 806, 1444, 1577
FriOI	GRGCYC	2 cut(s)	1532, 1814
Fsp4HI	GCNGC	6 cut(s)	234, 315, 888, 950, 1400, 1403
FspBI	CTAG	4 cut(s)	359, 390, 1710, 1824
FspI	TGCGCA	1 cut(s)	313
GlaI	GCGC	2 cut(s)	313, 1276
GluI	GCNGC	6 cut(s)	234, 315, 888, 950, 1400, 1403
GsuI	CTGGAG	1 cut(s)	347
HaeIII	GGCC	2 cut(s)	945, 1502
HapII	CCGG	5 cut(s)	29, 400, 1304, 1461, 1677
HgaI	GACGC	2 cut(s)	1010, 1194
HhaI	GCGC	2 cut(s)	314, 1277
Hin1II	CATG	9 cut(s)	13, 215, 271, 456, 1262, 1288, 1381, 1526, 1735
Hin6I	GCGC	2 cut(s)	312, 1275
HinP1I	GCGC	2 cut(s)	312, 1275
HincII	GTYRAC	1 cut(s)	1162
HindII	GTYRAC	1 cut(s)	1162
HindIII	AAGCTT	1 cut(s)	1240
HinfI	GANTC	5 cut(s)	413, 491, 584, 784, 982
HpaII	CCGG	5 cut(s)	29, 400, 1304, 1461, 1677
HphI	GGTGA	6 cut(s)	487, 691, 728, 1153, 1331, 1793
Hpy166II	GTNNAC	5 cut(s)	155, 364, 1162, 1475, 1539
Hpy188I	TCNGA	9 cut(s)	42, 107, 432, 460, 624, 973, 1074, 1129, 1807
Hpy188III	TCNNGA	6 cut(s)	10, 400, 1246, 1248, 1259, 1694
Hpy8I	GTNNAC	5 cut(s)	155, 364, 1162, 1475, 1539
Hpy99I	CGWCG	1 cut(s)	50
HpyAV	CCTTC	3 cut(s)	750, 1092, 1138
HpyCH4III	ACNGT	6 cut(s)	274, 567, 826, 897, 967, 1472
HpyCH4IV	ACGT	1 cut(s)	1186
HpyF10VI	GCNNNNNNNGC	5 cut(s)	506, 740, 942, 1387, 1396
HpyF3I	CTNAG	7 cut(s)	75, 459, 1071, 1176, 1619, 1752, 1881
HpySE526I	ACGT	1 cut(s)	1186
Hsp92II	CATG	9 cut(s)	13, 215, 271, 456, 1262, 1288, 1381, 1526, 1735
HspAI	GCGC	2 cut(s)	312, 1275
Kpn2I	TCCGGA	1 cut(s)	399
Kzo9I	GATC	7 cut(s)	20, 973, 1060, 1078, 1189, 1308, 1480
LmnI	GCTCC	4 cut(s)	402, 1496, 1512, 1527
Lsp1109I	GCAGC	4 cut(s)	220, 326, 961, 1389
LweI	GCATC	5 cut(s)	299, 402, 1360, 1466, 1555
MaeI	CTAG	4 cut(s)	359, 390, 1710, 1824
MaeII	ACGT	1 cut(s)	1186
MaeIII	GTNAC	3 cut(s)	260, 274, 1051
MalI	GATC	7 cut(s)	22, 975, 1062, 1080, 1191, 1310, 1482
MbiI	CCGCTC	1 cut(s)	1399
MboI	GATC	7 cut(s)	20, 973, 1060, 1078, 1189, 1308, 1480
MhlI	GDGCHC	4 cut(s)	157, 1072, 1532, 1814
MlsI	TGGCCA	1 cut(s)	945
MluCI	AATT	8 cut(s)	690, 875, 930, 939, 1038, 1097, 1340, 1870
MluNI	TGGCCA	1 cut(s)	945
MlyI	GAGTC	1 cut(s)	407
MmeI	TCCRAC	4 cut(s)	85, 879, 1627, 1633
MnlI	CCTC	9 cut(s)	147, 454, 497, 656, 952, 1317, 1634, 1851, 1860
Mox20I	TGGCCA	1 cut(s)	945
Mph1103I	ATGCAT	1 cut(s)	289
MroI	TCCGGA	1 cut(s)	399
MroXI	GAANNNNTTC	2 cut(s)	709, 1701
MscI	TGGCCA	1 cut(s)	945
MslI	CAYNNNNRTG	3 cut(s)	210, 594, 1283
Msp20I	TGGCCA	1 cut(s)	945
MspCI	CTTAAG	1 cut(s)	1408
MspI	CCGG	5 cut(s)	29, 400, 1304, 1461, 1677
MspR9I	CCNGG	9 cut(s)	222, 326, 1103, 1120, 1304, 1305, 1512, 1677, 1815
MvaI	CCWGG	6 cut(s)	222, 326, 1103, 1120, 1512, 1815
MvnI	CGCG	2 cut(s)	1205, 1275
MwoI	GCNNNNNNNGC	5 cut(s)	506, 740, 942, 1387, 1396
NciI	CCSGG	3 cut(s)	1304, 1305, 1677
NcoI	CCATGG	1 cut(s)	452
NdeII	GATC	7 cut(s)	20, 973, 1060, 1078, 1189, 1308, 1480
NlaIII	CATG	9 cut(s)	13, 215, 271, 456, 1262, 1288, 1381, 1526, 1735
NlaIV	GGNNCC	3 cut(s)	621, 1492, 1673
NmuCI	GTSAC	2 cut(s)	274, 1051
NsbI	TGCGCA	1 cut(s)	313
NsiI	ATGCAT	1 cut(s)	289
NspI	RCATGY	2 cut(s)	1288, 1381
PaeI	GCATGC	1 cut(s)	1381
PaeR7I	CTCGAG	1 cut(s)	1246
PagI	TCATGA	2 cut(s)	9, 1258
PasI	CCCWGGG	1 cut(s)	1814
PciI	ACATGT	1 cut(s)	1284
PcsI	WCGNNNNNNNCGW	1 cut(s)	921
PdmI	GAANNNNTTC	2 cut(s)	709, 1701
PfeI	GAWTC	4 cut(s)	491, 584, 784, 982
PfoI	TCCNGGA	1 cut(s)	1101
PkrI	GCNGC	6 cut(s)	235, 316, 889, 951, 1401, 1404
Ple19I	CGATCG	1 cut(s)	1063
PleI	GAGTC	1 cut(s)	407
PmaCI	CACGTG	1 cut(s)	1187
PmlI	CACGTG	1 cut(s)	1187
PpsI	GAGTC	1 cut(s)	407
Ppu21I	YACGTR	1 cut(s)	1187
PpuMI	RGGWCCY	1 cut(s)	1426
PscI	ACATGT	1 cut(s)	1284
PshBI	ATTAAT	1 cut(s)	1343
Psp124BI	GAGCTC	1 cut(s)	1532
Psp5II	RGGWCCY	1 cut(s)	1426
Psp6I	CCWGG	6 cut(s)	220, 324, 1101, 1118, 1510, 1813
PspCI	CACGTG	1 cut(s)	1187
PspGI	CCWGG	6 cut(s)	220, 324, 1101, 1118, 1510, 1813
PspN4I	GGNNCC	3 cut(s)	621, 1492, 1673
PspPI	GGNCC	3 cut(s)	227, 1426, 1500
PspPPI	RGGWCCY	1 cut(s)	1426
PstI	CTGCAG	3 cut(s)	165, 847, 1355
PstNI	CAGNNNCTG	2 cut(s)	169, 971
PvuI	CGATCG	1 cut(s)	1063
RsaI	GTAC	3 cut(s)	323, 335, 1474
RsaNI	GTAC	3 cut(s)	322, 334, 1473
RseI	CAYNNNNRTG	3 cut(s)	210, 594, 1283
SacI	GAGCTC	1 cut(s)	1532
SatI	GCNGC	6 cut(s)	234, 315, 888, 950, 1400, 1403
Sau3AI	GATC	7 cut(s)	20, 973, 1060, 1078, 1189, 1308, 1480
Sau96I	GGNCC	3 cut(s)	227, 1426, 1500
SbfI	CCTGCAGG	1 cut(s)	847
SchI	GAGTC	1 cut(s)	407
ScrFI	CCNGG	9 cut(s)	222, 326, 1103, 1120, 1304, 1305, 1512, 1677, 1815
SdaI	CCTGCAGG	1 cut(s)	847
SduI	GDGCHC	4 cut(s)	157, 1072, 1532, 1814
SfaNI	GCATC	5 cut(s)	299, 402, 1360, 1466, 1555
SfcI	CTRYAG	4 cut(s)	161, 843, 963, 1351
Sfr274I	CTCGAG	1 cut(s)	1246
SinI	GGWCC	2 cut(s)	227, 1426
SlaI	CTCGAG	1 cut(s)	1246
SmaI	CCCGGG	1 cut(s)	1305
SmiMI	CAYNNNNRTG	3 cut(s)	210, 594, 1283
SmlI	CTYRAG	3 cut(s)	1246, 1408, 1531
SmoI	CTYRAG	3 cut(s)	1246, 1408, 1531
SphI	GCATGC	1 cut(s)	1381
Sse8387I	CCTGCAGG	1 cut(s)	847
Sse9I	AATT	8 cut(s)	690, 875, 930, 939, 1038, 1097, 1340, 1870
SsiI	CCGC	6 cut(s)	225, 676, 887, 1203, 1273, 1399
SspMI	CTAG	4 cut(s)	359, 390, 1710, 1824
SstI	GAGCTC	1 cut(s)	1532
StyD4I	CCNGG	9 cut(s)	220, 324, 1101, 1118, 1302, 1303, 1510, 1675, 1813
StyI	CCWWGG	4 cut(s)	452, 504, 525, 1137
TaaI	ACNGT	6 cut(s)	274, 567, 826, 897, 967, 1472
TaiI	ACGT	1 cut(s)	1189
TaqI	TCGA	9 cut(s)	23, 307, 416, 489, 915, 1063, 1247, 1736, 1856
TaqII	GACCGA	1 cut(s)	59
TasI	AATT	8 cut(s)	690, 875, 930, 939, 1038, 1097, 1340, 1870
TatI	WGTACW	1 cut(s)	1472
TauI	GCSGC	2 cut(s)	890, 1402
TfiI	GAWTC	4 cut(s)	491, 584, 784, 982
TscAI	CASTG	3 cut(s)	488, 596, 856
TseFI	GTSAC	2 cut(s)	274, 1051
TseI	GCWGC	4 cut(s)	233, 314, 949, 1402
Tsp45I	GTSAC	2 cut(s)	274, 1051
TspDTI	ATGAA	6 cut(s)	17, 26, 201, 213, 1029, 1720
TspGWI	ACGGA	1 cut(s)	63
TspMI	CCCGGG	1 cut(s)	1303
TspRI	CASTG	3 cut(s)	488, 596, 856
Vha464I	CTTAAG	1 cut(s)	1408
VneI	GTGCAC	1 cut(s)	153
VpaK11BI	GGWCC	2 cut(s)	227, 1426
VspI	ATTAAT	1 cut(s)	1343
XagI	CCTNNNNNAGG	1 cut(s)	1855
XapI	RAATTY	2 cut(s)	1097, 1870
XceI	RCATGY	2 cut(s)	1288, 1381
XcmI	CCANNNNNNNNNTGG	2 cut(s)	218, 1144
XhoI	CTCGAG	1 cut(s)	1246
XmaI	CCCGGG	1 cut(s)	1303
XmiI	GTMKAC	2 cut(s)	363, 1538
XmnI	GAANNNNTTC	2 cut(s)	709, 1701
XspI	CTAG	4 cut(s)	359, 390, 1710, 1824
Zsp2I	ATGCAT	1 cut(s)	289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.