Rh2BG208300

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0003992
Physical Location & Seq
Reverse (-)
19478333 .. 19483599
5267 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG208300.1

Sequence Viewer

Length: 1608 bp
ATGACAGTAACTCTCAATGCATACAATGTGGTTGCAACTCGATGCGCAGCAGAGTACCTTGAGATGTATGAAACTGTTGAGAAAGGAAACCTTGTCTACAAGGTTGAAGTTTTCCTAAGCTCTAGCATCTTCCGAAGCTGGAAAGACTCGATAATTGTTCTTCAGACAACAAGGTCTCTTCTTCCCTGGGCTGAGGAACTTAAGGTTGTCAGCCATTGCCTAGACTCCATAGCATCCAAGGCTACCATTGATACTTCCAAGGTGGAATGGTCATATTCGTATAACCATAAAAAGCTGCCATCTGAGAATGGGAATGATCCACTCTGGAATGGTGTAGGAAAACAGCAGATGGTTCCAAAAGACTGGTGGGTAGAAGACCTCTGTGGGCTTCAACTTGATTTATACAAGCGGGTGATAACAATAATCAAGACCAAAGGGAAAATCTCTGCTGATGTAATTGGGGAAGCCTTAAATGCATACACTGTCAGAAGGTTATCAGGTATTAGTAAGGGTATGATTCAGGGAGGTGATGTCTTAAAGAATAGATCATTGGTGGAGACTGTCATCCACCTGCTTCCTACAGGGAGTGTCCCCTGCAGTTTCTTACTTAAGTTGTTAAAAGCAGCTATCTTGTTAGAATGTGGAGAAATGGAAAAAAGTGAATTGATGAGGAGAATTAGCCAGCAGCTTGAGGAGGCCACAGTTTCTGATCTTTTAATTCGAGCCCCAGCTGAGGAAGAAATGATATATGATGTTGATATAGTGCACAGGCTAATTGAAGAGTTCGTGGCACGTGATCAAAGTGCTCAGATAGATCCTTCAATGGAAACTGAATTCCAGGAGATCAAAACCCCCAGGTTCCTATCAGATACTTCAAAGGTGAAGGTGGCAAAGCTGGTTGATGGCTACCTTGCCGAAACTGGACGTGATCCCAAGTTACTGGTGTCAGAGTTTGTCAGTCTTGCTGAAATTGTATCAAGCTTCCCGCGATCATCTCATGATGGAGTTTACCGTGCCATTGACATGTTTCTAAAGGAACACCCGGGAATCAGCAAGAGTGAAAAGAAGAGAATATGCAAATTAATGGACTGTAGGAAGTTATCAGCAGCAGCCTGCACACATGCTGTGCAAAATGAGCGGCTGCCCCTGAGAGTCGTTGTGCAGGTCCTTTTCTTTGAACAGGCTAGGGCTTCCGTTGGTGGAAACAGTACACCTGATCTACATGGATCCATCAGAGCCTTACTCCCTGGTGGGTCTCATGGAAGCTCAAGATCCACTATAACCAACACAGAAGAGGACTGGGATGCTGTGCCAACCGCCGAGGAGATCAAGGCTTTAAAAGGGGAGATATCTACACTACGGTTAGATGATGGTAATGAGGGAGTTGGCAATGATAAGAATGATCATCATGGTGCTAAAAACCATTCTGAAAAAGTTGCTGCCAGCAAAATGAAAGGGTTACTGATGTCAAAGAAGATATTCTCTAAGCTCTGGTCAAACAAGGAAAGGAATGGTGAAAGCAGCTCGGATACATCGGAGAGTCCTAGTTCTATCAATGCTGAAGAAACAAAATCCCATTCCAGGAGTAGGAGGCGCTCATTATCTTAG

Protein Analysis

535

Amino Acids

59.52

Weight (kDa)

6.7

Isoelectric Point (pI)

41.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 121 - 372 1.4e-84 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 579
AasI GACNNNNNNGTC 1 cut(s) 172
Acc16I TGCGCA 1 cut(s) 46
Acc36I ACCTGC 2 cut(s) 579, 1153
AccBSI CCGCTC 1 cut(s) 1138
AccI GTMKAC 1 cut(s) 96
AccII CGCG 1 cut(s) 988
AciI CCGC 4 cut(s) 409, 986, 1138, 1317
AclWI GGATC 6 cut(s) 311, 809, 923, 1221, 1234, 1266
AcsI RAATTY 1 cut(s) 833
AcuI CTGAAG 2 cut(s) 146, 1581
AcvI CACGTG 1 cut(s) 794
AfaI GTAC 2 cut(s) 56, 1210
AfiI CCNNNNNNNGG 3 cut(s) 733, 1581, 1587
AflII CTTAAG 2 cut(s) 200, 608
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 6 cut(s) 107, 392, 779, 822, 876, 1178
AjiI CACGTC 1 cut(s) 926
AjnI CCWGG 5 cut(s) 185, 837, 854, 1246, 1580
AjuI GAANNNNNNNTTGG 2 cut(s) 533, 565
Alw21I GWGCWC 2 cut(s) 768, 808
Alw26I GTCTC 3 cut(s) 180, 551, 1260
Alw44I GTGCAC 1 cut(s) 764
AlwI GGATC 6 cut(s) 311, 809, 923, 1221, 1234, 1266
AlwNI CAGNNNCTG 1 cut(s) 707
Ama87I CYCGRG 1 cut(s) 1042
AoxI GGCC 1 cut(s) 696
ApaLI GTGCAC 1 cut(s) 764
ApeKI GCWGC 9 cut(s) 47, 295, 623, 685, 1106, 1109, 1141, 1439, 1521
ApoI RAATTY 1 cut(s) 833
AseI ATTAAT 1 cut(s) 1082
Asp700I GAANNNNTTC 1 cut(s) 1478
AspLEI GCGC 2 cut(s) 47, 1596
AspS9I GGNCC 1 cut(s) 1165
AsuC2I CCSGG 2 cut(s) 1043, 1044
AsuHPI GGTGA 4 cut(s) 424, 539, 892, 1526
AvaI CYCGRG 1 cut(s) 1042
AvaII GGWCC 1 cut(s) 1165
BaeGI GKGCMC 1 cut(s) 768
BamHI GGATCC 1 cut(s) 1226
BanII GRGCYC 1 cut(s) 727
BbrPI CACGTG 1 cut(s) 794
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 2 cut(s) 768, 808
BbvCI CCTCAGC 2 cut(s) 192, 732
BbvI GCAGC 9 cut(s) 59, 282, 635, 697, 1118, 1121, 1128, 1426, 1533
BccI CCATC 6 cut(s) 307, 343, 896, 995, 1238, 1364
BciT130I CCWGG 5 cut(s) 187, 839, 856, 1248, 1582
BciVI GTATCC 1 cut(s) 1522
BclI TGATCA 2 cut(s) 796, 1402
BcnI CCSGG 2 cut(s) 1043, 1044
BcoDI GTCTC 3 cut(s) 180, 551, 1260
BfaI CTAG 4 cut(s) 123, 221, 1185, 1545
BfmI CTRYAG 3 cut(s) 579, 595, 1090
BfoI RGCGCY 1 cut(s) 1597
BfrI CTTAAG 2 cut(s) 200, 608
BfuAI ACCTGC 2 cut(s) 579, 1153
BfuI GTATCC 1 cut(s) 1522
Bme1390I CCNGG 7 cut(s) 187, 839, 856, 1043, 1044, 1248, 1582
Bme18I GGWCC 1 cut(s) 1165
BmeT110I CYCGRG 1 cut(s) 1042
BmgBI CACGTC 1 cut(s) 926
BmgT120I GGNCC 1 cut(s) 1165
BmiI GGNNCC 3 cut(s) 354, 860, 1228
BmrFI CCNGG 7 cut(s) 187, 839, 856, 1043, 1044, 1248, 1582
BmrI ACTGGG 1 cut(s) 1309
BmsI GCATC 4 cut(s) 32, 135, 242, 1294
BmuI ACTGGG 1 cut(s) 1309
BpiI GAAGAC 1 cut(s) 381
BplI GAGNNNNNCTC 2 cut(s) 1227, 1259
Bpu10I CCTNAGC 3 cut(s) 116, 192, 732
BpuEI CTTGAG 3 cut(s) 80, 710, 1252
BpuMI CCSGG 2 cut(s) 1043, 1044
BsaAI YACGTR 1 cut(s) 794
BsaI GGTCTC 2 cut(s) 180, 1260
BsaJI CCNNGG 8 cut(s) 185, 186, 237, 258, 854, 1042, 1246, 1320
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 3 cut(s) 733, 1581, 1587
Bse1I ACTGG 4 cut(s) 368, 925, 945, 1304
Bse3DI GCAATG 2 cut(s) 214, 1396
BseBI CCWGG 5 cut(s) 187, 839, 856, 1248, 1582
BseDI CCNNGG 8 cut(s) 185, 186, 237, 258, 854, 1042, 1246, 1320
BseGI GGATG 3 cut(s) 233, 564, 1309
BseLI CCNNNNNNNGG 3 cut(s) 733, 1581, 1587
BseMI GCAATG 2 cut(s) 214, 1396
BseMII CTCAG 5 cut(s) 183, 294, 723, 821, 1139
BseNI ACTGG 4 cut(s) 368, 925, 945, 1304
BseRI GAGGAG 3 cut(s) 685, 707, 1337
BseSI GKGCMC 1 cut(s) 768
BseXI GCAGC 9 cut(s) 59, 282, 635, 697, 1118, 1121, 1128, 1426, 1533
BseYI CCCAGC 1 cut(s) 727
BsgI GTGCAG 2 cut(s) 1099, 1181
Bsh1236I CGCG 1 cut(s) 988
BshFI GGCC 1 cut(s) 698
BsiHKAI GWGCWC 2 cut(s) 768, 808
BsiHKCI CYCGRG 1 cut(s) 1042
BsiSI CCGG 1 cut(s) 1043
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 3 cut(s) 733, 1581, 1587
BsmAI GTCTC 3 cut(s) 180, 551, 1260
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 1 cut(s) 698
Bso31I GGTCTC 2 cut(s) 180, 1260
BsoBI CYCGRG 1 cut(s) 1042
Bsp1286I GDGCHC 3 cut(s) 727, 768, 808
BspACI CCGC 4 cut(s) 409, 986, 1138, 1317
BspANI GGCC 1 cut(s) 698
BspCNI CTCAG 5 cut(s) 184, 295, 724, 820, 1140
BspFNI CGCG 1 cut(s) 988
BspHI TCATGA 1 cut(s) 997
BspLI GGNNCC 3 cut(s) 354, 860, 1228
BspMAI CTGCAG 1 cut(s) 599
BspMI ACCTGC 2 cut(s) 579, 1153
BspPI GGATC 6 cut(s) 311, 809, 923, 1221, 1234, 1266
BspTI CTTAAG 2 cut(s) 200, 608
BspTNI GGTCTC 2 cut(s) 180, 1260
BsrBI CCGCTC 1 cut(s) 1138
BsrDI GCAATG 2 cut(s) 214, 1396
BsrI ACTGG 4 cut(s) 368, 925, 945, 1304
BssECI CCNNGG 8 cut(s) 185, 186, 237, 258, 854, 1042, 1246, 1320
BssT1I CCWWGG 2 cut(s) 237, 258
Bst2UI CCWGG 5 cut(s) 187, 839, 856, 1248, 1582
Bst4CI ACNGT 9 cut(s) 7, 76, 484, 562, 703, 1013, 1091, 1208, 1362
Bst6I CTCTTC 4 cut(s) 183, 774, 1061, 1287
BstAFI CTTAAG 2 cut(s) 200, 608
BstBAI YACGTR 1 cut(s) 794
BstC8I GCNNGC 3 cut(s) 683, 1114, 1444
BstDEI CTNAG 8 cut(s) 116, 192, 303, 732, 807, 1148, 1485, 1605
BstF5I GGATG 3 cut(s) 233, 564, 1309
BstFNI CGCG 1 cut(s) 988
BstH2I RGCGCY 1 cut(s) 1597
BstHHI GCGC 2 cut(s) 47, 1596
BstMAI GTCTC 3 cut(s) 180, 551, 1260
BstMWI GCNNNNNNNGC 3 cut(s) 239, 473, 1135
BstNI CCWGG 5 cut(s) 187, 839, 856, 1248, 1582
BstNSI RCATGY 2 cut(s) 1027, 1124
BstSCI CCNGG 7 cut(s) 185, 837, 854, 1041, 1042, 1246, 1580
BstSFI CTRYAG 3 cut(s) 579, 595, 1090
BstSLI GKGCMC 1 cut(s) 768
BstUI CGCG 1 cut(s) 988
BstV1I GCAGC 9 cut(s) 59, 282, 635, 697, 1118, 1121, 1128, 1426, 1533
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 3 cut(s) 814, 1226, 1271
BstXI CCANNNNNNTGG 2 cut(s) 363, 940
BstYI RGATCY 3 cut(s) 814, 1226, 1271
BsuI GTATCC 1 cut(s) 1522
BsuRI GGCC 1 cut(s) 698
BtrI CACGTC 1 cut(s) 926
BtsCI GGATG 3 cut(s) 233, 564, 1309
BtsIMutI CAGTG 1 cut(s) 480
BveI ACCTGC 2 cut(s) 579, 1153
Cac8I GCNNGC 3 cut(s) 683, 1114, 1444
CaiI CAGNNNCTG 1 cut(s) 707
CciI TCATGA 1 cut(s) 997
CfoI GCGC 2 cut(s) 47, 1596
Cfr13I GGNCC 1 cut(s) 1165
Cfr9I CCCGGG 1 cut(s) 1042
Csp6I GTAC 2 cut(s) 55, 1209
CviAII CATG 6 cut(s) 998, 1024, 1121, 1223, 1259, 1409
CviQI GTAC 2 cut(s) 55, 1209
DdeI CTNAG 8 cut(s) 116, 192, 303, 732, 807, 1148, 1485, 1605
DraI TTTAAA 1 cut(s) 1338
DrdI GACNNNNNNGTC 1 cut(s) 172
DseDI GACNNNNNNGTC 1 cut(s) 172
Eam1104I CTCTTC 4 cut(s) 183, 774, 1061, 1287
EarI CTCTTC 4 cut(s) 183, 774, 1061, 1287
Eco130I CCWWGG 2 cut(s) 237, 258
Eco24I GRGCYC 1 cut(s) 727
Eco31I GGTCTC 2 cut(s) 180, 1260
Eco32I GATATC 1 cut(s) 1350
Eco47I GGWCC 1 cut(s) 1165
Eco57I CTGAAG 2 cut(s) 146, 1581
Eco72I CACGTG 1 cut(s) 794
Eco88I CYCGRG 1 cut(s) 1042
EcoO109I RGGNCCY 1 cut(s) 1165
EcoRI GAATTC 1 cut(s) 833
EcoRII CCWGG 5 cut(s) 185, 837, 854, 1246, 1580
EcoRV GATATC 1 cut(s) 1350
EcoT14I CCWWGG 2 cut(s) 237, 258
EcoT22I ATGCAT 2 cut(s) 22, 478
EcoT38I GRGCYC 1 cut(s) 727
ErhI CCWWGG 2 cut(s) 237, 258
FaeI CATG 6 cut(s) 1001, 1027, 1124, 1226, 1262, 1412
FalI AAGNNNNNCTT 2 cut(s) 75, 107
FaqI GGGAC 1 cut(s) 575
FatI CATG 6 cut(s) 997, 1023, 1120, 1222, 1258, 1408
FauI CCCGC 2 cut(s) 402, 993
FbaI TGATCA 2 cut(s) 796, 1402
FblI GTMKAC 1 cut(s) 96
FokI GGATG 3 cut(s) 220, 551, 1316
FriOI GRGCYC 1 cut(s) 727
FspBI CTAG 4 cut(s) 123, 221, 1185, 1545
FspI TGCGCA 1 cut(s) 46
GlaI GCGC 2 cut(s) 46, 1595
GsaI CCCAGC 1 cut(s) 731
HaeII RGCGCY 1 cut(s) 1597
HaeIII GGCC 1 cut(s) 698
HapII CCGG 1 cut(s) 1043
HhaI GCGC 2 cut(s) 47, 1596
Hin1II CATG 6 cut(s) 1001, 1027, 1124, 1226, 1262, 1412
Hin6I GCGC 2 cut(s) 45, 1594
HinP1I GCGC 2 cut(s) 45, 1594
HindIII AAGCTT 1 cut(s) 979
HinfI GANTC 6 cut(s) 146, 224, 517, 1047, 1152, 1540
HpaII CCGG 1 cut(s) 1043
HphI GGTGA 4 cut(s) 424, 539, 892, 1526
Hpy166II GTNNAC 4 cut(s) 97, 766, 1009, 1211
Hpy188III TCNNGA 4 cut(s) 325, 427, 998, 1269
Hpy8I GTNNAC 4 cut(s) 97, 766, 1009, 1211
HpyAV CCTTC 3 cut(s) 483, 828, 877
HpyCH4III ACNGT 9 cut(s) 7, 76, 484, 562, 703, 1013, 1091, 1208, 1362
HpyCH4IV ACGT 2 cut(s) 793, 925
HpyCH4V TGCA 9 cut(s) 20, 35, 476, 597, 766, 1077, 1116, 1129, 1162
HpyF10VI GCNNNNNNNGC 3 cut(s) 239, 473, 1135
HpyF3I CTNAG 8 cut(s) 116, 192, 303, 732, 807, 1148, 1485, 1605
HpySE526I ACGT 2 cut(s) 793, 925
Hsp92II CATG 6 cut(s) 1001, 1027, 1124, 1226, 1262, 1412
HspAI GCGC 2 cut(s) 45, 1594
Ksp22I TGATCA 2 cut(s) 796, 1402
Lsp1109I GCAGC 9 cut(s) 59, 282, 635, 697, 1118, 1121, 1128, 1426, 1533
LweI GCATC 4 cut(s) 32, 135, 242, 1294
MaeI CTAG 4 cut(s) 123, 221, 1185, 1545
MaeII ACGT 2 cut(s) 793, 925
MaeIII GTNAC 3 cut(s) 7, 936, 1458
MbiI CCGCTC 1 cut(s) 1138
MflI RGATCY 3 cut(s) 814, 1226, 1271
MhlI GDGCHC 3 cut(s) 727, 768, 808
MluCI AATT 9 cut(s) 153, 456, 662, 675, 717, 774, 833, 969, 1079
MlyI GAGTC 4 cut(s) 140, 218, 1161, 1549
Mph1103I ATGCAT 2 cut(s) 22, 478
MroXI GAANNNNTTC 1 cut(s) 1478
MseI TTAA 8 cut(s) 201, 470, 536, 609, 617, 716, 1082, 1337
MslI CAYNNNNRTG 2 cut(s) 1022, 1410
MspA1I CMGCKG 1 cut(s) 731
MspCI CTTAAG 2 cut(s) 200, 608
MspI CCGG 1 cut(s) 1043
MspR9I CCNGG 7 cut(s) 187, 839, 856, 1043, 1044, 1248, 1582
MvaI CCWGG 5 cut(s) 187, 839, 856, 1248, 1582
MvnI CGCG 1 cut(s) 988
MwoI GCNNNNNNNGC 3 cut(s) 239, 473, 1135
NciI CCSGG 2 cut(s) 1043, 1044
NlaIII CATG 6 cut(s) 1001, 1027, 1124, 1226, 1262, 1412
NlaIV GGNNCC 3 cut(s) 354, 860, 1228
NmeAIII GCCGAG 1 cut(s) 1345
NsbI TGCGCA 1 cut(s) 46
NsiI ATGCAT 2 cut(s) 22, 478
NspI RCATGY 2 cut(s) 1027, 1124
PagI TCATGA 1 cut(s) 997
PaqCI CACCTGC 1 cut(s) 579
PasI CCCWGGG 1 cut(s) 186
PciI ACATGT 1 cut(s) 1023
PdmI GAANNNNTTC 1 cut(s) 1478
PfeI GAWTC 2 cut(s) 517, 1047
PfoI TCCNGGA 2 cut(s) 837, 1580
PleI GAGTC 4 cut(s) 140, 218, 1160, 1548
PmaCI CACGTG 1 cut(s) 794
PmlI CACGTG 1 cut(s) 794
PpsI GAGTC 4 cut(s) 140, 218, 1160, 1548
Ppu21I YACGTR 1 cut(s) 794
PpuMI RGGWCCY 1 cut(s) 1165
PscI ACATGT 1 cut(s) 1023
PshBI ATTAAT 1 cut(s) 1082
Psp5II RGGWCCY 1 cut(s) 1165
Psp6I CCWGG 5 cut(s) 185, 837, 854, 1246, 1580
PspCI CACGTG 1 cut(s) 794
PspFI CCCAGC 1 cut(s) 727
PspGI CCWGG 5 cut(s) 185, 837, 854, 1246, 1580
PspN4I GGNNCC 3 cut(s) 354, 860, 1228
PspPI GGNCC 1 cut(s) 1165
PspPPI RGGWCCY 1 cut(s) 1165
PstI CTGCAG 1 cut(s) 599
PstNI CAGNNNCTG 1 cut(s) 707
PsuI RGATCY 3 cut(s) 814, 1226, 1271
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 2 cut(s) 56, 1210
RsaNI GTAC 2 cut(s) 55, 1209
RseI CAYNNNNRTG 2 cut(s) 1022, 1410
SaqAI TTAA 8 cut(s) 201, 470, 536, 609, 617, 716, 1082, 1337
Sau96I GGNCC 1 cut(s) 1165
SchI GAGTC 4 cut(s) 140, 218, 1161, 1549
ScrFI CCNGG 7 cut(s) 187, 839, 856, 1043, 1044, 1248, 1582
SduI GDGCHC 3 cut(s) 727, 768, 808
SfaNI GCATC 4 cut(s) 32, 135, 242, 1294
SfcI CTRYAG 3 cut(s) 579, 595, 1090
SinI GGWCC 1 cut(s) 1165
SmaI CCCGGG 1 cut(s) 1044
SmiMI CAYNNNNRTG 2 cut(s) 1022, 1410
SmlI CTYRAG 5 cut(s) 59, 200, 608, 689, 1267
SmoI CTYRAG 5 cut(s) 59, 200, 608, 689, 1267
Sse9I AATT 9 cut(s) 153, 456, 662, 675, 717, 774, 833, 969, 1079
SsiI CCGC 4 cut(s) 409, 986, 1138, 1317
SspMI CTAG 4 cut(s) 123, 221, 1185, 1545
StyD4I CCNGG 7 cut(s) 185, 837, 854, 1041, 1042, 1246, 1580
StyI CCWWGG 2 cut(s) 237, 258
TaaI ACNGT 9 cut(s) 7, 76, 484, 562, 703, 1013, 1091, 1208, 1362
TaiI ACGT 2 cut(s) 796, 928
TaqI TCGA 3 cut(s) 40, 149, 721
TasI AATT 9 cut(s) 153, 456, 662, 675, 717, 774, 833, 969, 1079
TatI WGTACW 1 cut(s) 1208
TauI GCSGC 1 cut(s) 1141
TfiI GAWTC 2 cut(s) 517, 1047
Tru1I TTAA 8 cut(s) 201, 470, 536, 609, 617, 716, 1082, 1337
Tru9I TTAA 8 cut(s) 201, 470, 536, 609, 617, 716, 1082, 1337
TscAI CASTG 1 cut(s) 487
TseI GCWGC 9 cut(s) 47, 295, 623, 685, 1106, 1109, 1141, 1439, 1521
TspDTI ATGAA 2 cut(s) 84, 1466
TspGWI ACGGA 1 cut(s) 1183
TspMI CCCGGG 1 cut(s) 1042
TspRI CASTG 1 cut(s) 487
Vha464I CTTAAG 2 cut(s) 200, 608
VneI GTGCAC 1 cut(s) 764
VpaK11BI GGWCC 1 cut(s) 1165
VspI ATTAAT 1 cut(s) 1082
XapI RAATTY 1 cut(s) 833
XceI RCATGY 2 cut(s) 1027, 1124
XcmI CCANNNNNNNNNTGG 1 cut(s) 363
XmaI CCCGGG 1 cut(s) 1042
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 1478
XspI CTAG 4 cut(s) 123, 221, 1185, 1545
Zsp2I ATGCAT 2 cut(s) 22, 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.