Rroxscaffold_2G00136090

Belongs to the NPH3 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003992
Physical Location & Seq
Forward (+)
73656790 .. 73661867
5078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136090.1

Sequence Viewer

Length: 960 bp
ATGGAAAAAAGTGAATTGATGAGGAGAATTAGCCAGCAGCTTGAGGAGGCTACAGTTTCTGATCTTTTAATTCGAGCCCCAGCTGAGGAAGAAATGATATATGATGTTGATATAGTGCACAGGCTAATTGAAGAGTTCGTGTCACGTGATCAAAGTGCTCAGACAGATCCTTCAATGGAAACTGAATTCCAGGAGATCAAAACCCCCAGGTTCCTATCAGATACTTCAAAGGTGAAGGTGGCAAAGCTGGTTGATGGCTACCTTGCCGAAACTGGACGTGATCCCAAGTTACCGGTGTCAGAGTTTGTCAGTCTTGCTGAAATTGTATCAAGCTTCCCGCGATCATCTCATGATGGAGTTTACCGTGCCATTGACATGTTTCTAAAGGAACACCCAGGAATCAGCAAGAGCGAAAAGAAGAGAATATGCAAATTAATGGACTGTAGGAAGTTATCAGCAGCAGCCTGCACACATGCCGTGCAAAATGAGCGGCTGCCCCTGAGAGTCGTTGTGCAGGTCCTTTTCTTCGAACAGGCTAGGGCTTCAGTAGGTGGAAACAGTACACCTGATCTACATGGATCCATCAGAGCCTTACTCCCTGGTGGGTCTCATGGAAGCTCAAGATCCACTACAACCAACACAGAAGAGGACTGGGATGCTGTGCCAACCGCCGAGGAGATCAAGGCTTTAAAAGGGGAGATATCTACACTACGGTTAGGTGATGGTAATGAGGGAGTTGGCAATGATAAGAATGATCATCATGGTGCTAAAAACCATTCTGAAAAAGTTGCTGCCAGCAAAATGAAAGGGTTACTGATGTCAAAGAAGATATTCTCTAAGCTCTGGTCAAACAAGGAAAGGAATGGTGAAAGCAGCTCGGATACATCGGAGAGTCATAGTTCTATCAATGCTGAAGAAACAAAATCCCATTCCAGGAGTAGGAGGCGCTCATTATCTTAG

Protein Analysis

319

Amino Acids

35.38

Weight (kDa)

6.44

Isoelectric Point (pI)

49.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NPH3 PF03000 3 - 156 1.5e-55 NPH3 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 505
AccBSI CCGCTC 1 cut(s) 490
AccII CGCG 1 cut(s) 340
AciI CCGC 3 cut(s) 338, 490, 669
AclWI GGATC 5 cut(s) 161, 275, 573, 586, 618
AcsI RAATTY 1 cut(s) 185
AcuI CTGAAG 2 cut(s) 528, 933
AcvI CACGTG 1 cut(s) 146
AfaI GTAC 1 cut(s) 562
AfiI CCNNNNNNNGG 3 cut(s) 85, 933, 939
AflIII ACRYGT 1 cut(s) 375
AgeI ACCGGT 1 cut(s) 292
AgsI TTSAA 3 cut(s) 131, 174, 228
AjiI CACGTC 1 cut(s) 278
AjnI CCWGG 5 cut(s) 189, 206, 394, 598, 932
AluBI AGCT 7 cut(s) 40, 83, 247, 333, 618, 841, 876
AluI AGCT 7 cut(s) 40, 83, 247, 333, 618, 841, 876
Alw21I GWGCWC 2 cut(s) 120, 160
Alw26I GTCTC 1 cut(s) 612
Alw44I GTGCAC 1 cut(s) 116
AlwI GGATC 5 cut(s) 161, 275, 573, 586, 618
AlwNI CAGNNNCTG 1 cut(s) 59
ApaLI GTGCAC 1 cut(s) 116
ApeKI GCWGC 6 cut(s) 37, 458, 461, 493, 791, 873
ApoI RAATTY 1 cut(s) 185
AseI ATTAAT 1 cut(s) 434
AsiGI ACCGGT 1 cut(s) 292
Asp700I GAANNNNTTC 1 cut(s) 830
AspLEI GCGC 1 cut(s) 948
AspS9I GGNCC 1 cut(s) 517
AsuHPI GGTGA 3 cut(s) 244, 731, 878
AsuII TTCGAA 1 cut(s) 528
AvaII GGWCC 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 120
BamHI GGATCC 1 cut(s) 578
BanII GRGCYC 1 cut(s) 79
BbrPI CACGTG 1 cut(s) 146
Bbv12I GWGCWC 2 cut(s) 120, 160
BbvCI CCTCAGC 1 cut(s) 84
BbvI GCAGC 6 cut(s) 49, 470, 473, 480, 778, 885
BccI CCATC 4 cut(s) 248, 347, 590, 716
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 5 cut(s) 191, 208, 396, 600, 934
BciVI GTATCC 1 cut(s) 874
BclI TGATCA 2 cut(s) 148, 754
BcoDI GTCTC 1 cut(s) 612
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 2 cut(s) 51, 442
BfoI RGCGCY 1 cut(s) 949
BfuAI ACCTGC 1 cut(s) 505
BfuI GTATCC 1 cut(s) 874
BisI GCNGC 7 cut(s) 38, 459, 462, 491, 494, 792, 874
BlsI GCNGC 7 cut(s) 39, 460, 463, 492, 495, 793, 875
Bme1390I CCNGG 5 cut(s) 191, 208, 396, 600, 934
Bme18I GGWCC 1 cut(s) 517
BmgBI CACGTC 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 517
BmiI GGNNCC 2 cut(s) 212, 580
BmrFI CCNGG 5 cut(s) 191, 208, 396, 600, 934
BmrI ACTGGG 1 cut(s) 661
BmsI GCATC 1 cut(s) 646
BmuI ACTGGG 1 cut(s) 661
BplI GAGNNNNNCTC 2 cut(s) 579, 611
Bpu10I CCTNAGC 1 cut(s) 84
Bpu14I TTCGAA 1 cut(s) 528
BpuEI CTTGAG 2 cut(s) 62, 604
BsaAI YACGTR 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 612
BsaJI CCNNGG 4 cut(s) 206, 394, 598, 672
BsaWI WCCGGW 1 cut(s) 292
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 933, 939
Bse118I RCCGGY 1 cut(s) 292
Bse1I ACTGG 2 cut(s) 277, 656
Bse3DI GCAATG 1 cut(s) 748
BseBI CCWGG 5 cut(s) 191, 208, 396, 600, 934
BseDI CCNNGG 4 cut(s) 206, 394, 598, 672
BseGI GGATG 1 cut(s) 661
BseLI CCNNNNNNNGG 3 cut(s) 85, 933, 939
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 3 cut(s) 75, 173, 491
BseNI ACTGG 2 cut(s) 277, 656
BseRI GAGGAG 3 cut(s) 37, 59, 689
BseSI GKGCMC 1 cut(s) 120
BseXI GCAGC 6 cut(s) 49, 470, 473, 480, 778, 885
BseYI CCCAGC 1 cut(s) 79
BsgI GTGCAG 2 cut(s) 451, 533
Bsh1236I CGCG 1 cut(s) 340
BshTI ACCGGT 1 cut(s) 292
BsiHKAI GWGCWC 2 cut(s) 120, 160
BsiSI CCGG 1 cut(s) 293
BslI CCNNNNNNNGG 3 cut(s) 85, 933, 939
BsmAI GTCTC 1 cut(s) 612
Bso31I GGTCTC 1 cut(s) 612
Bsp119I TTCGAA 1 cut(s) 528
Bsp1286I GDGCHC 3 cut(s) 79, 120, 160
BspACI CCGC 3 cut(s) 338, 490, 669
BspCNI CTCAG 3 cut(s) 76, 172, 492
BspFNI CGCG 1 cut(s) 340
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 2 cut(s) 212, 580
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 5 cut(s) 161, 275, 573, 586, 618
BspT104I TTCGAA 1 cut(s) 528
BspTNI GGTCTC 1 cut(s) 612
BsrBI CCGCTC 1 cut(s) 490
BsrDI GCAATG 1 cut(s) 748
BsrFI RCCGGY 1 cut(s) 292
BsrI ACTGG 2 cut(s) 277, 656
BssAI RCCGGY 1 cut(s) 292
BssECI CCNNGG 4 cut(s) 206, 394, 598, 672
Bst2UI CCWGG 5 cut(s) 191, 208, 396, 600, 934
Bst4CI ACNGT 5 cut(s) 55, 365, 443, 560, 714
Bst6I CTCTTC 3 cut(s) 126, 413, 639
BstBAI YACGTR 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 528
BstC8I GCNNGC 3 cut(s) 35, 466, 796
BstDEI CTNAG 5 cut(s) 84, 159, 500, 837, 957
BstF5I GGATG 1 cut(s) 661
BstFNI CGCG 1 cut(s) 340
BstH2I RGCGCY 1 cut(s) 949
BstHHI GCGC 1 cut(s) 948
BstMAI GTCTC 1 cut(s) 612
BstMWI GCNNNNNNNGC 1 cut(s) 487
BstNI CCWGG 5 cut(s) 191, 208, 396, 600, 934
BstNSI RCATGY 2 cut(s) 379, 476
BstSCI CCNGG 5 cut(s) 189, 206, 394, 598, 932
BstSFI CTRYAG 2 cut(s) 51, 442
BstSLI GKGCMC 1 cut(s) 120
BstUI CGCG 1 cut(s) 340
BstV1I GCAGC 6 cut(s) 49, 470, 473, 480, 778, 885
BstX2I RGATCY 3 cut(s) 166, 578, 623
BstYI RGATCY 3 cut(s) 166, 578, 623
BsuI GTATCC 1 cut(s) 874
BtrI CACGTC 1 cut(s) 278
BtsCI GGATG 1 cut(s) 661
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 3 cut(s) 35, 466, 796
CaiI CAGNNNCTG 1 cut(s) 59
CciI TCATGA 1 cut(s) 349
CfoI GCGC 1 cut(s) 948
Cfr10I RCCGGY 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 517
Csp6I GTAC 1 cut(s) 561
CspAI ACCGGT 1 cut(s) 292
CviAII CATG 6 cut(s) 350, 376, 473, 575, 611, 761
CviQI GTAC 1 cut(s) 561
DdeI CTNAG 5 cut(s) 84, 159, 500, 837, 957
DraI TTTAAA 1 cut(s) 690
Eam1104I CTCTTC 3 cut(s) 126, 413, 639
EarI CTCTTC 3 cut(s) 126, 413, 639
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 612
Eco32I GATATC 1 cut(s) 702
Eco47I GGWCC 1 cut(s) 517
Eco57I CTGAAG 2 cut(s) 528, 933
Eco72I CACGTG 1 cut(s) 146
EcoO109I RGGNCCY 1 cut(s) 517
EcoRI GAATTC 1 cut(s) 185
EcoRII CCWGG 5 cut(s) 189, 206, 394, 598, 932
EcoRV GATATC 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 79
FaeI CATG 6 cut(s) 353, 379, 476, 578, 614, 764
FatI CATG 6 cut(s) 349, 375, 472, 574, 610, 760
FauI CCCGC 1 cut(s) 345
FbaI TGATCA 2 cut(s) 148, 754
Fnu4HI GCNGC 7 cut(s) 38, 459, 462, 491, 494, 792, 874
FokI GGATG 1 cut(s) 668
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 7 cut(s) 38, 459, 462, 491, 494, 792, 874
FspBI CTAG 1 cut(s) 537
GlaI GCGC 1 cut(s) 947
GluI GCNGC 7 cut(s) 38, 459, 462, 491, 494, 792, 874
GsaI CCCAGC 1 cut(s) 83
HaeII RGCGCY 1 cut(s) 949
HapII CCGG 1 cut(s) 293
HhaI GCGC 1 cut(s) 948
Hin1II CATG 6 cut(s) 353, 379, 476, 578, 614, 764
Hin6I GCGC 1 cut(s) 946
HinP1I GCGC 1 cut(s) 946
HindIII AAGCTT 1 cut(s) 331
HinfI GANTC 3 cut(s) 399, 504, 892
HpaII CCGG 1 cut(s) 293
HphI GGTGA 3 cut(s) 244, 731, 878
Hpy166II GTNNAC 3 cut(s) 118, 361, 563
Hpy188I TCNGA 8 cut(s) 61, 162, 220, 301, 587, 781, 880, 889
Hpy188III TCNNGA 2 cut(s) 350, 621
Hpy8I GTNNAC 3 cut(s) 118, 361, 563
HpyAV CCTTC 2 cut(s) 180, 229
HpyCH4III ACNGT 5 cut(s) 55, 365, 443, 560, 714
HpyCH4IV ACGT 2 cut(s) 145, 277
HpyCH4V TGCA 5 cut(s) 118, 429, 468, 481, 514
HpyF10VI GCNNNNNNNGC 1 cut(s) 487
HpyF3I CTNAG 5 cut(s) 84, 159, 500, 837, 957
HpySE526I ACGT 2 cut(s) 145, 277
Hsp92II CATG 6 cut(s) 353, 379, 476, 578, 614, 764
HspAI GCGC 1 cut(s) 946
Ksp22I TGATCA 2 cut(s) 148, 754
Lsp1109I GCAGC 6 cut(s) 49, 470, 473, 480, 778, 885
LweI GCATC 1 cut(s) 646
MaeI CTAG 1 cut(s) 537
MaeII ACGT 2 cut(s) 145, 277
MaeIII GTNAC 3 cut(s) 141, 288, 810
MbiI CCGCTC 1 cut(s) 490
MboII GAAGA 7 cut(s) 101, 143, 430, 517, 656, 838, 926
MflI RGATCY 3 cut(s) 166, 578, 623
MhlI GDGCHC 3 cut(s) 79, 120, 160
MluCI AATT 7 cut(s) 14, 27, 69, 126, 185, 321, 431
MlyI GAGTC 2 cut(s) 513, 901
MnlI CCTC 8 cut(s) 15, 37, 40, 79, 640, 667, 724, 936
MroXI GAANNNNTTC 1 cut(s) 830
MseI TTAA 3 cut(s) 68, 434, 689
MslI CAYNNNNRTG 2 cut(s) 374, 762
MspA1I CMGCKG 1 cut(s) 83
MspI CCGG 1 cut(s) 293
MspR9I CCNGG 5 cut(s) 191, 208, 396, 600, 934
MvaI CCWGG 5 cut(s) 191, 208, 396, 600, 934
MvnI CGCG 1 cut(s) 340
MwoI GCNNNNNNNGC 1 cut(s) 487
NlaIII CATG 6 cut(s) 353, 379, 476, 578, 614, 764
NlaIV GGNNCC 2 cut(s) 212, 580
NmeAIII GCCGAG 1 cut(s) 697
NmuCI GTSAC 1 cut(s) 141
NspI RCATGY 2 cut(s) 379, 476
NspV TTCGAA 1 cut(s) 528
PagI TCATGA 1 cut(s) 349
PciI ACATGT 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 830
PfeI GAWTC 1 cut(s) 399
PfoI TCCNGGA 2 cut(s) 189, 932
PinAI ACCGGT 1 cut(s) 292
PkrI GCNGC 7 cut(s) 39, 460, 463, 492, 495, 793, 875
PleI GAGTC 2 cut(s) 512, 900
PmaCI CACGTG 1 cut(s) 146
PmlI CACGTG 1 cut(s) 146
PpsI GAGTC 2 cut(s) 512, 900
Ppu21I YACGTR 1 cut(s) 146
PpuMI RGGWCCY 1 cut(s) 517
PscI ACATGT 1 cut(s) 375
PshBI ATTAAT 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 517
Psp6I CCWGG 5 cut(s) 189, 206, 394, 598, 932
PspCI CACGTG 1 cut(s) 146
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 5 cut(s) 189, 206, 394, 598, 932
PspN4I GGNNCC 2 cut(s) 212, 580
PspPI GGNCC 1 cut(s) 517
PspPPI RGGWCCY 1 cut(s) 517
PstNI CAGNNNCTG 1 cut(s) 59
PsuI RGATCY 3 cut(s) 166, 578, 623
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 1 cut(s) 562
RsaNI GTAC 1 cut(s) 561
RseI CAYNNNNRTG 2 cut(s) 374, 762
SaqAI TTAA 3 cut(s) 68, 434, 689
SatI GCNGC 7 cut(s) 38, 459, 462, 491, 494, 792, 874
Sau96I GGNCC 1 cut(s) 517
SchI GAGTC 2 cut(s) 513, 901
ScrFI CCNGG 5 cut(s) 191, 208, 396, 600, 934
SduI GDGCHC 3 cut(s) 79, 120, 160
SfaNI GCATC 1 cut(s) 646
SfcI CTRYAG 2 cut(s) 51, 442
SfuI TTCGAA 1 cut(s) 528
SinI GGWCC 1 cut(s) 517
SmiMI CAYNNNNRTG 2 cut(s) 374, 762
SmlI CTYRAG 2 cut(s) 41, 619
SmoI CTYRAG 2 cut(s) 41, 619
Sse9I AATT 7 cut(s) 14, 27, 69, 126, 185, 321, 431
SsiI CCGC 3 cut(s) 338, 490, 669
SspMI CTAG 1 cut(s) 537
StyD4I CCNGG 5 cut(s) 189, 206, 394, 598, 932
TaaI ACNGT 5 cut(s) 55, 365, 443, 560, 714
TaiI ACGT 2 cut(s) 148, 280
TaqI TCGA 2 cut(s) 73, 528
TasI AATT 7 cut(s) 14, 27, 69, 126, 185, 321, 431
TatI WGTACW 1 cut(s) 560
TauI GCSGC 1 cut(s) 493
TfiI GAWTC 1 cut(s) 399
Tru1I TTAA 3 cut(s) 68, 434, 689
Tru9I TTAA 3 cut(s) 68, 434, 689
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 6 cut(s) 37, 458, 461, 493, 791, 873
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 1 cut(s) 818
VneI GTGCAC 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 517
VspI ATTAAT 1 cut(s) 434
XapI RAATTY 1 cut(s) 185
XceI RCATGY 2 cut(s) 379, 476
XmnI GAANNNNTTC 1 cut(s) 830
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.