Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0003992

Physical Location & Seq

Genomic Coordinates Reverse (-)

27661371 .. 27665081

Gene Structure 3711 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1295200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1881 bp

ATGAAGTTCATGAAACTTGGATCGAAACCGGATTTCTTTCAGACCGATGGAGACACTATAAGGTACGTCACAACTGAGTTGGCAACTGACATAGCTGTTAATGTCGGAGATGTGAAGTTCTATTTGCATAAGTTTCCTCTTTTGTCCAAGAGTGCACGCCTGCAGAAACTGGTTGCAACCACAAATGAAAACAGCGATGAAATCCACATCCATGATATTCCTGGTGGACCTGCTGCTTTTGAAATATGTGCCAAGTTCTGTTACGGCATGACTGTCACTCTCAATGCATACAATGTGGTTGCAACTCGATGCGCAGCAGAGTATCTGGAGATGTACGAAACTATTGAGAAAGGAAACCTAGTCTACAAGGTTGAAGTGTTCCTAAACTCTAGCGTCTTCCGGAGCTGGAAAGACTCGATTATTGTTCTTCAGACTACGAGGTCTCTTGTACCATGGTCCGAGGAAGTTAAGGTGGTGAGCCACTGCCTTGATTCCATAGCTTCCAAGGCTACCATTGATACTTCCAAGGTTGAATGGTCGTATATGTATAACCACAAGAAACTACCATCTAATGGGAATGATTCACACTGGAATGGAGTGGCAAAACAACAGATGGTTCCGAAAGACTGGTGGGTGGAAGACCTCTGTGGGCTTCAACTTGATTTATACAAGCGGGTGATAACAATAATCAAGACCAAAGGAAAAATCTCTGGTGATGTAATAGGGGAAGCGTTAAAGGCATATATTACCAGAAGGTTGTCAGATTTTAGCAAGGGCATGATTCAGGGCGATGTTGTAAAGAATAAATCATTGGTGGAGACTGTAATCCACTTACTCCCTGCACGCAGTGTCCCTTGCAGTTTCTTACTTAAATTGTTAAAAGCGGCAAGACTGTTAGAATGTGGAGAAGTCGAAAAATGTGAGTTAATTCGCAAAATTGGCCAGCAGCTTGACGAGGCTACAGTTTCTGATCTTTTGATTCAACCCCCAACTGATGAAACAATAAAATATGAAGTTGACATGGTGCAAAGCCTAATTGAAGAGTTTGTGACACACAATCGAAGTGCTCAGACTGATCCTTCAATGGAAACCGAATTCCAGGAGATTAGAAGTCCCAGGTTATCAGATACTTCCAAGGTGAAGGTGGCCAAGCTGGTTGACAGCTACCTTACTGAGATTTCATGTGATCCCAACTTACCTGCGTCAAAGTTTGTCCATCTTGCTGAAATGGTATCAAGCTTCTCAAGACCAACTCATGATGGACTTTACCGTGCCATTGACATGTATCTAAAGGAACACCCAGGGATCAGCAAGGGTGAGAAGAAGAGAATGTGCAAATTAATGGACTGCAGGAAGTTGTCAGTAGATGCCTGCATGCATGCTGTGCAAAATGAGCGGCTACCCTTAAGAGTCGTTGCGCAGGTACTCTTCTTTGAACAGATTAGGGCTTCAGCGTCCGGTGGCAGCACCAGTATACCTGATCTGCAAGGCTCCATCTGGGCCTTACTCCCAGGTGGGTCTCATGGGAGCTCAAGGTCAACCACAACCAACACAGAAGACGACTGGGATGATGTGCCGACAGCAGAAGAAATCAAGGCTTTAAAATGTGAGATTTTCACTCCTAGGCTAGGCGAAGTGAATGGAGGAGTTGGCAGTGATAGAAATGGGAACCCCGGTGCTACAAAAAATCTTGAAAAAGTTCCATCTGGTAAAGTGAAAGGTATGATCATGTCGAAAAAGATATTTTCTAAGCTGTGGTCAAGCAAAGAAAGCAGTGGTGAAATCAGCAGTTGTAATACATCAGAGAGCCCTGGTTCTTCTCGCAATGCAGAAGAAAAAAAGTCCACCCGTTCGAGGAGTAGGAGGAATTCGCTGTCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005623 GO:0007275 GO:0008150 GO:0009605 GO:0009606 GO:0009628 GO:0009629 GO:0009630 GO:0009791 GO:0009908 GO:0009958 GO:0022414 GO:0032501 GO:0032502 GO:0044464 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0061458 GO:0071944 GO:0090567

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BTB NPH3

Protein Analysis

627

Amino Acids

69.71

Weight (kDa)

8.06

Isoelectric Point (pI)

43.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	27 - 117	9.4e-07	BTB/POZ domain
NPH3	PF03000	209 - 458	5.5e-85	NPH3 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003992)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G23050 AT2G23050 AT4G37590 AT5G67440 AT5G67440 AT5G67440
fragaria_vesca	FvH4_1g17440 FvH4_1g17440 FvH4_1g17440 FvH4_1g17440 FvH4_1g17440 FvH4_1g17440 FvH4_1g17440
malus_domestica	MD02G1183500.v1.1 MD15G1295200.v1.1
prunus_persica	Prupe.6G222500_v2.0.a1 Prupe.6G222500_v2.0.a1 Prupe.6G222500_v2.0.a1 Prupe.6G222500_v2.0.a1 Prupe.6G222500_v2.0.a1
pyrus_communis	pycom02g14920 pycom15g25820
rosa_chinensis	RchiOBHm_Chr2g0107651
rosa_laevigata	RLG00000017605
rosa_roxburghii	Rroxscaffold_2G00136090
rosa_rugosa	Rorug02G0146100 Rorug02G0146100
rosa_samantha	Rh2AG195900 Rh2BG208300 Rh2CG201000 Rh2DG203400 Rh6AG399700
rosa_wichuraiana	Rw2G015500

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	439
Acc16I	TGCGCA	2 cut(s)	313, 1419
Acc36I	ACCTGC	3 cut(s)	238, 1207, 1411
AccB7I	CCANNNNNTGG	1 cut(s)	572
AccBSI	CCGCTC	1 cut(s)	1396
AccI	GTMKAC	2 cut(s)	363, 1474
AccIII	TCCGGA	1 cut(s)	399
AciI	CCGC	3 cut(s)	673, 884, 1396
AclWI	GGATC	4 cut(s)	28, 1070, 1181, 1313
AcoI	YGGCCR	2 cut(s)	940, 1146
AcsI	RAATTY	2 cut(s)	1094, 1867
AcuI	CTGAAG	2 cut(s)	413, 1434
AdeI	CACNNNGTG	1 cut(s)	848
AfaI	GTAC	4 cut(s)	65, 335, 450, 1425
AfiI	CCNNNNNNNGG	3 cut(s)	572, 1628, 1854
AflII	CTTAAG	1 cut(s)	1405
AflIII	ACRYGT	1 cut(s)	1281
AgsI	TTSAA	9 cut(s)	242, 374, 533, 656, 983, 1040, 1083, 1436, 1694
AjnI	CCWGG	6 cut(s)	220, 1098, 1115, 1300, 1510, 1810
AjuI	GAANNNNNNNTTGG	2 cut(s)	794, 826
AluBI	AGCT	9 cut(s)	95, 405, 500, 949, 1153, 1164, 1239, 1530, 1753
AluI	AGCT	9 cut(s)	95, 405, 500, 949, 1153, 1164, 1239, 1530, 1753
Alw21I	GWGCWC	3 cut(s)	157, 1069, 1532
Alw26I	GTCTC	4 cut(s)	45, 447, 812, 1524
Alw44I	GTGCAC	1 cut(s)	153
AlwI	GGATC	4 cut(s)	28, 1070, 1181, 1313
AlwNI	CAGNNNCTG	2 cut(s)	169, 968
Aor13HI	TCCGGA	1 cut(s)	399
AoxI	GGCC	3 cut(s)	940, 1146, 1500
ApaLI	GTGCAC	1 cut(s)	153
ApeKI	GCWGC	4 cut(s)	233, 314, 946, 1464
ApoI	RAATTY	2 cut(s)	1094, 1867
AseI	ATTAAT	1 cut(s)	1340
Asp700I	GAANNNNTTC	1 cut(s)	1698
AspA2I	CCTAGG	1 cut(s)	1622
AspLEI	GCGC	2 cut(s)	314, 1420
AspS9I	GGNCC	3 cut(s)	227, 456, 1500
AsuC2I	CCSGG	1 cut(s)	1674
AsuHPI	GGTGA	6 cut(s)	487, 688, 725, 1150, 1328, 1790
AvaII	GGWCC	2 cut(s)	227, 456
AvrII	CCTAGG	1 cut(s)	1622
BaeGI	GKGCMC	1 cut(s)	157
BalI	TGGCCA	2 cut(s)	942, 1148
BanII	GRGCYC	2 cut(s)	1532, 1811
BbsI	GAAGAC	3 cut(s)	388, 645, 1563
Bbv12I	GWGCWC	3 cut(s)	157, 1069, 1532
BbvI	GCAGC	4 cut(s)	220, 326, 958, 1476
BccI	CCATC	7 cut(s)	41, 574, 607, 1224, 1253, 1502, 1711
BceAI	ACGGC	1 cut(s)	280
BciT130I	CCWGG	6 cut(s)	222, 1100, 1117, 1302, 1512, 1812
BclI	TGATCA	1 cut(s)	1725
BcnI	CCSGG	1 cut(s)	1674
BcoDI	GTCTC	4 cut(s)	45, 447, 812, 1524
BfaI	CTAG	4 cut(s)	359, 390, 1623, 1628
BfmI	CTRYAG	3 cut(s)	161, 960, 1348
BfrI	CTTAAG	1 cut(s)	1405
BfuAI	ACCTGC	3 cut(s)	238, 1207, 1411
BisI	GCNGC	6 cut(s)	234, 315, 885, 947, 1397, 1465
BlnI	CCTAGG	1 cut(s)	1622
BlsI	GCNGC	6 cut(s)	235, 316, 886, 948, 1398, 1466
Bme1390I	CCNGG	7 cut(s)	222, 1100, 1117, 1302, 1512, 1674, 1812
Bme18I	GGWCC	2 cut(s)	227, 456
BmgT120I	GGNCC	3 cut(s)	227, 456, 1500
BmiI	GGNNCC	3 cut(s)	618, 1492, 1670
BmrFI	CCNGG	7 cut(s)	222, 1100, 1117, 1302, 1512, 1674, 1812
BmrI	ACTGGG	1 cut(s)	1573
BmsI	GCATC	2 cut(s)	299, 1357
BmuI	ACTGGG	1 cut(s)	1573
BpiI	GAAGAC	3 cut(s)	388, 645, 1563
BpmI	CTGGAG	1 cut(s)	347
BpuEI	CTTGAG	2 cut(s)	1228, 1516
BpuMI	CCSGG	1 cut(s)	1674
BsaI	GGTCTC	2 cut(s)	447, 1524
BsaWI	WCCGGW	3 cut(s)	28, 399, 1457
Bsc4I	CCNNNNNNNGG	3 cut(s)	572, 1628, 1854
Bse1I	ACTGG	5 cut(s)	174, 593, 632, 1470, 1568
Bse3DI	GCAATG	1 cut(s)	1831
BseAI	TCCGGA	1 cut(s)	399
BseBI	CCWGG	6 cut(s)	222, 1100, 1117, 1302, 1512, 1812
BseGI	GGATG	2 cut(s)	207, 1573
BseLI	CCNNNNNNNGG	3 cut(s)	572, 1628, 1854
BseMI	GCAATG	1 cut(s)	1831
BseMII	CTCAG	3 cut(s)	66, 1082, 1164
BseNI	ACTGG	5 cut(s)	174, 593, 632, 1470, 1568
BseRI	GAGGAG	2 cut(s)	1659, 1870
BseSI	GKGCMC	1 cut(s)	157
BseXI	GCAGC	4 cut(s)	220, 326, 958, 1476
BsgI	GTGCAG	1 cut(s)	825
BshFI	GGCC	3 cut(s)	942, 1148, 1502
BsiHKAI	GWGCWC	3 cut(s)	157, 1069, 1532
BsiSI	CCGG	4 cut(s)	29, 400, 1458, 1674
BslFI	GGGAC	2 cut(s)	836, 1098
BslI	CCNNNNNNNGG	3 cut(s)	572, 1628, 1854
BsmAI	GTCTC	4 cut(s)	45, 447, 812, 1524
BsmFI	GGGAC	2 cut(s)	836, 1098
BsnI	GGCC	3 cut(s)	942, 1148, 1502
Bso31I	GGTCTC	2 cut(s)	447, 1524
Bsp1286I	GDGCHC	4 cut(s)	157, 1069, 1532, 1811
Bsp13I	TCCGGA	1 cut(s)	399
Bsp143I	GATC	7 cut(s)	20, 970, 1075, 1186, 1305, 1480, 1725
Bsp19I	CCATGG	1 cut(s)	452
BspACI	CCGC	3 cut(s)	673, 884, 1396
BspANI	GGCC	3 cut(s)	942, 1148, 1502
BspCNI	CTCAG	3 cut(s)	67, 1081, 1165
BspEI	TCCGGA	1 cut(s)	399
BspHI	TCATGA	2 cut(s)	9, 1255
BspLI	GGNNCC	3 cut(s)	618, 1492, 1670
BspMAI	CTGCAG	2 cut(s)	165, 1352
BspMI	ACCTGC	3 cut(s)	238, 1207, 1411
BspPI	GGATC	4 cut(s)	28, 1070, 1181, 1313
BspTI	CTTAAG	1 cut(s)	1405
BspTNI	GGTCTC	2 cut(s)	447, 1524
BsrBI	CCGCTC	1 cut(s)	1396
BsrDI	GCAATG	1 cut(s)	1831
BsrI	ACTGG	5 cut(s)	174, 593, 632, 1470, 1568
BssMI	GATC	7 cut(s)	20, 970, 1075, 1186, 1305, 1480, 1725
BssNAI	GTATAC	1 cut(s)	1475
BssT1I	CCWWGG	5 cut(s)	452, 504, 525, 1134, 1622
Bst1107I	GTATAC	1 cut(s)	1475
Bst2UI	CCWGG	6 cut(s)	222, 1100, 1117, 1302, 1512, 1812
Bst4CI	ACNGT	5 cut(s)	274, 823, 894, 964, 1271
Bst6I	CTCTTC	3 cut(s)	1035, 1319, 1433
BstAFI	CTTAAG	1 cut(s)	1405
BstAPI	GCANNNNNTGC	1 cut(s)	1384
BstC8I	GCNNGC	7 cut(s)	157, 161, 844, 944, 1372, 1376, 1380
BstDEI	CTNAG	5 cut(s)	75, 1068, 1173, 1749, 1878
BstDSI	CCRYGG	1 cut(s)	452
BstENI	CCTNNNNNAGG	1 cut(s)	1626
BstF5I	GGATG	2 cut(s)	207, 1573
BstHHI	GCGC	2 cut(s)	314, 1420
BstKTI	GATC	7 cut(s)	23, 973, 1078, 1189, 1308, 1483, 1728
BstMAI	GTCTC	4 cut(s)	45, 447, 812, 1524
BstMBI	GATC	7 cut(s)	20, 970, 1075, 1186, 1305, 1480, 1725
BstMWI	GCNNNNNNNGC	6 cut(s)	506, 737, 939, 1384, 1393, 1770
BstNI	CCWGG	6 cut(s)	222, 1100, 1117, 1302, 1512, 1812
BstNSI	RCATGY	3 cut(s)	1285, 1378, 1382
BstSCI	CCNGG	7 cut(s)	220, 1098, 1115, 1300, 1510, 1672, 1810
BstSFI	CTRYAG	3 cut(s)	161, 960, 1348
BstSLI	GKGCMC	1 cut(s)	157
BstV1I	GCAGC	4 cut(s)	220, 326, 958, 1476
BstV2I	GAAGAC	3 cut(s)	388, 645, 1563
BstZ17I	GTATAC	1 cut(s)	1475
BsuRI	GGCC	3 cut(s)	942, 1148, 1502
BtgI	CCRYGG	1 cut(s)	452
BtgZI	GCGATG	2 cut(s)	210, 804
BtsCI	GGATG	2 cut(s)	207, 1573
BtsI	GCAGTG	4 cut(s)	481, 853, 1660, 1780
BtsIMutI	CAGTG	5 cut(s)	481, 586, 853, 1660, 1780
BveI	ACCTGC	3 cut(s)	238, 1207, 1411
Cac8I	GCNNGC	7 cut(s)	157, 161, 844, 944, 1372, 1376, 1380
CaiI	CAGNNNCTG	2 cut(s)	169, 968
CciI	TCATGA	2 cut(s)	9, 1255
CfoI	GCGC	2 cut(s)	314, 1420
Cfr13I	GGNCC	3 cut(s)	227, 456, 1500
CseI	GACGC	3 cut(s)	382, 1191, 1443
Csp6I	GTAC	4 cut(s)	64, 334, 449, 1424
CviQI	GTAC	4 cut(s)	64, 334, 449, 1424
DdeI	CTNAG	5 cut(s)	75, 1068, 1173, 1749, 1878
DpnI	GATC	7 cut(s)	22, 972, 1077, 1188, 1307, 1482, 1727
DpnII	GATC	7 cut(s)	20, 970, 1075, 1186, 1305, 1480, 1725
DraI	TTTAAA	1 cut(s)	1602
DraIII	CACNNNGTG	1 cut(s)	848
DrdI	GACNNNNNNGTC	1 cut(s)	439
DseDI	GACNNNNNNGTC	1 cut(s)	439
EaeI	YGGCCR	2 cut(s)	940, 1146
Eam1104I	CTCTTC	3 cut(s)	1035, 1319, 1433
EarI	CTCTTC	3 cut(s)	1035, 1319, 1433
Ecl136II	GAGCTC	1 cut(s)	1530
Eco130I	CCWWGG	5 cut(s)	452, 504, 525, 1134, 1622
Eco24I	GRGCYC	2 cut(s)	1532, 1811
Eco31I	GGTCTC	2 cut(s)	447, 1524
Eco47I	GGWCC	2 cut(s)	227, 456
Eco53kI	GAGCTC	1 cut(s)	1530
Eco57I	CTGAAG	2 cut(s)	413, 1434
EcoICRI	GAGCTC	1 cut(s)	1530
EcoNI	CCTNNNNNAGG	1 cut(s)	1626
EcoRI	GAATTC	2 cut(s)	1094, 1867
EcoRII	CCWGG	6 cut(s)	220, 1098, 1115, 1300, 1510, 1810
EcoT14I	CCWWGG	5 cut(s)	452, 504, 525, 1134, 1622
EcoT22I	ATGCAT	2 cut(s)	289, 1380
EcoT38I	GRGCYC	2 cut(s)	1532, 1811
ErhI	CCWWGG	5 cut(s)	452, 504, 525, 1134, 1622
FaqI	GGGAC	2 cut(s)	836, 1098
FauI	CCCGC	1 cut(s)	666
FbaI	TGATCA	1 cut(s)	1725
FblI	GTMKAC	2 cut(s)	363, 1474
Fnu4HI	GCNGC	6 cut(s)	234, 315, 885, 947, 1397, 1465
FokI	GGATG	2 cut(s)	194, 1580
FriOI	GRGCYC	2 cut(s)	1532, 1811
Fsp4HI	GCNGC	6 cut(s)	234, 315, 885, 947, 1397, 1465
FspBI	CTAG	4 cut(s)	359, 390, 1623, 1628
FspI	TGCGCA	2 cut(s)	313, 1419
GlaI	GCGC	2 cut(s)	313, 1419
GluI	GCNGC	6 cut(s)	234, 315, 885, 947, 1397, 1465
GsuI	CTGGAG	1 cut(s)	347
HaeIII	GGCC	3 cut(s)	942, 1148, 1502
HapII	CCGG	4 cut(s)	29, 400, 1458, 1674
HgaI	GACGC	3 cut(s)	382, 1191, 1443
HhaI	GCGC	2 cut(s)	314, 1420
Hin6I	GCGC	2 cut(s)	312, 1418
HinP1I	GCGC	2 cut(s)	312, 1418
HincII	GTYRAC	3 cut(s)	1018, 1159, 1539
HindII	GTYRAC	3 cut(s)	1018, 1159, 1539
HindIII	AAGCTT	1 cut(s)	1237
HinfI	GANTC	6 cut(s)	413, 491, 581, 781, 979, 1410
HpaII	CCGG	4 cut(s)	29, 400, 1458, 1674
HphI	GGTGA	6 cut(s)	487, 688, 725, 1150, 1328, 1790
Hpy166II	GTNNAC	8 cut(s)	155, 227, 364, 1018, 1159, 1475, 1539, 1845
Hpy188III	TCNNGA	7 cut(s)	10, 326, 400, 691, 1245, 1256, 1691
Hpy8I	GTNNAC	8 cut(s)	155, 227, 364, 1018, 1159, 1475, 1539, 1845
HpyAV	CCTTC	3 cut(s)	747, 1089, 1135
HpyCH4III	ACNGT	5 cut(s)	274, 823, 894, 964, 1271
HpyCH4IV	ACGT	1 cut(s)	66
HpyF10VI	GCNNNNNNNGC	6 cut(s)	506, 737, 939, 1384, 1393, 1770
HpyF3I	CTNAG	5 cut(s)	75, 1068, 1173, 1749, 1878
HpySE526I	ACGT	1 cut(s)	66
HspAI	GCGC	2 cut(s)	312, 1418
Kpn2I	TCCGGA	1 cut(s)	399
Ksp22I	TGATCA	1 cut(s)	1725
Kzo9I	GATC	7 cut(s)	20, 970, 1075, 1186, 1305, 1480, 1725
LmnI	GCTCC	3 cut(s)	402, 1496, 1527
Lsp1109I	GCAGC	4 cut(s)	220, 326, 958, 1476
LweI	GCATC	2 cut(s)	299, 1357
MaeI	CTAG	4 cut(s)	359, 390, 1623, 1628
MaeII	ACGT	1 cut(s)	66
MaeIII	GTNAC	4 cut(s)	67, 260, 274, 1048
MalI	GATC	7 cut(s)	22, 972, 1077, 1188, 1307, 1482, 1727
MbiI	CCGCTC	1 cut(s)	1396
MboI	GATC	7 cut(s)	20, 970, 1075, 1186, 1305, 1480, 1725
MhlI	GDGCHC	4 cut(s)	157, 1069, 1532, 1811
MlsI	TGGCCA	2 cut(s)	942, 1148
MluCI	AATT	7 cut(s)	872, 927, 936, 1035, 1094, 1337, 1867
MluNI	TGGCCA	2 cut(s)	942, 1148
MlyI	GAGTC	2 cut(s)	407, 1419
MmeI	TCCRAC	1 cut(s)	85
MnlI	CCTC	8 cut(s)	147, 432, 454, 653, 949, 1637, 1848, 1857
Mox20I	TGGCCA	2 cut(s)	942, 1148
Mph1103I	ATGCAT	2 cut(s)	289, 1380
MroI	TCCGGA	1 cut(s)	399
MroXI	GAANNNNTTC	1 cut(s)	1698
MscI	TGGCCA	2 cut(s)	942, 1148
MseI	TTAA	9 cut(s)	99, 468, 734, 870, 878, 926, 1340, 1406, 1601
MslI	CAYNNNNRTG	3 cut(s)	210, 591, 1280
Msp20I	TGGCCA	2 cut(s)	942, 1148
MspCI	CTTAAG	1 cut(s)	1405
MspI	CCGG	4 cut(s)	29, 400, 1458, 1674
MspR9I	CCNGG	7 cut(s)	222, 1100, 1117, 1302, 1512, 1674, 1812
MvaI	CCWGG	6 cut(s)	222, 1100, 1117, 1302, 1512, 1812
MwoI	GCNNNNNNNGC	6 cut(s)	506, 737, 939, 1384, 1393, 1770
NciI	CCSGG	1 cut(s)	1674
NcoI	CCATGG	1 cut(s)	452
NdeII	GATC	7 cut(s)	20, 970, 1075, 1186, 1305, 1480, 1725
NlaIV	GGNNCC	3 cut(s)	618, 1492, 1670
NmuCI	GTSAC	3 cut(s)	67, 274, 1048
NsbI	TGCGCA	2 cut(s)	313, 1419
NsiI	ATGCAT	2 cut(s)	289, 1380
NspI	RCATGY	3 cut(s)	1285, 1378, 1382
PaeI	GCATGC	2 cut(s)	1378, 1382
PagI	TCATGA	2 cut(s)	9, 1255
PasI	CCCWGGG	1 cut(s)	1301
PciI	ACATGT	1 cut(s)	1281
PdmI	GAANNNNTTC	1 cut(s)	1698
PfeI	GAWTC	4 cut(s)	491, 581, 781, 979
PflMI	CCANNNNNTGG	1 cut(s)	572
PfoI	TCCNGGA	1 cut(s)	1098
PkrI	GCNGC	6 cut(s)	235, 316, 886, 948, 1398, 1466
PleI	GAGTC	2 cut(s)	407, 1418
PpsI	GAGTC	2 cut(s)	407, 1418
PscI	ACATGT	1 cut(s)	1281
PshBI	ATTAAT	1 cut(s)	1340
Psp124BI	GAGCTC	1 cut(s)	1532
Psp6I	CCWGG	6 cut(s)	220, 1098, 1115, 1300, 1510, 1810
PspGI	CCWGG	6 cut(s)	220, 1098, 1115, 1300, 1510, 1810
PspN4I	GGNNCC	3 cut(s)	618, 1492, 1670
PspPI	GGNCC	3 cut(s)	227, 456, 1500
PstI	CTGCAG	2 cut(s)	165, 1352
PstNI	CAGNNNCTG	2 cut(s)	169, 968
RsaI	GTAC	4 cut(s)	65, 335, 450, 1425
RsaNI	GTAC	4 cut(s)	64, 334, 449, 1424
RseI	CAYNNNNRTG	3 cut(s)	210, 591, 1280
SacI	GAGCTC	1 cut(s)	1532
SaqAI	TTAA	9 cut(s)	99, 468, 734, 870, 878, 926, 1340, 1406, 1601
SatI	GCNGC	6 cut(s)	234, 315, 885, 947, 1397, 1465
Sau3AI	GATC	7 cut(s)	20, 970, 1075, 1186, 1305, 1480, 1725
Sau96I	GGNCC	3 cut(s)	227, 456, 1500
SchI	GAGTC	2 cut(s)	407, 1419
ScrFI	CCNGG	7 cut(s)	222, 1100, 1117, 1302, 1512, 1674, 1812
SduI	GDGCHC	4 cut(s)	157, 1069, 1532, 1811
SfaNI	GCATC	2 cut(s)	299, 1357
SfcI	CTRYAG	3 cut(s)	161, 960, 1348
SinI	GGWCC	2 cut(s)	227, 456
SmiMI	CAYNNNNRTG	3 cut(s)	210, 591, 1280
SmlI	CTYRAG	3 cut(s)	1243, 1405, 1531
SmoI	CTYRAG	3 cut(s)	1243, 1405, 1531
SphI	GCATGC	2 cut(s)	1378, 1382
Sse9I	AATT	7 cut(s)	872, 927, 936, 1035, 1094, 1337, 1867
SsiI	CCGC	3 cut(s)	673, 884, 1396
SspMI	CTAG	4 cut(s)	359, 390, 1623, 1628
SstI	GAGCTC	1 cut(s)	1532
StyD4I	CCNGG	7 cut(s)	220, 1098, 1115, 1300, 1510, 1672, 1810
StyI	CCWWGG	5 cut(s)	452, 504, 525, 1134, 1622
TaaI	ACNGT	5 cut(s)	274, 823, 894, 964, 1271
TaiI	ACGT	1 cut(s)	69
TaqI	TCGA	7 cut(s)	23, 307, 416, 912, 1060, 1733, 1853
TaqII	GACCGA	1 cut(s)	59
TasI	AATT	7 cut(s)	872, 927, 936, 1035, 1094, 1337, 1867
TauI	GCSGC	2 cut(s)	887, 1399
TfiI	GAWTC	4 cut(s)	491, 581, 781, 979
Tru1I	TTAA	9 cut(s)	99, 468, 734, 870, 878, 926, 1340, 1406, 1601
Tru9I	TTAA	9 cut(s)	99, 468, 734, 870, 878, 926, 1340, 1406, 1601
TscAI	CASTG	5 cut(s)	488, 593, 853, 1660, 1780
TseFI	GTSAC	3 cut(s)	67, 274, 1048
TseI	GCWGC	4 cut(s)	233, 314, 946, 1464
Tsp45I	GTSAC	3 cut(s)	67, 274, 1048
TspDTI	ATGAA	7 cut(s)	17, 26, 201, 213, 1011, 1026, 1170
TspRI	CASTG	5 cut(s)	488, 593, 853, 1660, 1780
Van91I	CCANNNNNTGG	1 cut(s)	572
Vha464I	CTTAAG	1 cut(s)	1405
VneI	GTGCAC	1 cut(s)	153
VpaK11BI	GGWCC	2 cut(s)	227, 456
VspI	ATTAAT	1 cut(s)	1340
XagI	CCTNNNNNAGG	1 cut(s)	1626
XapI	RAATTY	2 cut(s)	1094, 1867
XceI	RCATGY	3 cut(s)	1285, 1378, 1382
XcmI	CCANNNNNNNNNTGG	2 cut(s)	218, 1141
XmaJI	CCTAGG	1 cut(s)	1622
XmiI	GTMKAC	2 cut(s)	363, 1474
XmnI	GAANNNNTTC	1 cut(s)	1698
XspI	CTAG	4 cut(s)	359, 390, 1623, 1628
Zsp2I	ATGCAT	2 cut(s)	289, 1380

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.