AT2G27430

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013290
Physical Location & Seq
Reverse (-)
11729759 .. 11733371
3613 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27430.1

Sequence Viewer

Length: 1317 bp
ATGTCTTCCTCTCGAACACAATCCTCTTCTTCTTCAGTTTGGCAACTTCAATACATGAAGCTCAACTTCTTCACAAAGATTCGATGCCTTTTGAAATCCAAAGCATCCTTCCGCAAAAGTAATCTTCAAGCGTTACCAGAGAAATCAAGAACCTATCAAGATTTCGAGAAGGTGGCGGCGCTTCCGGAAGAAATTGTATCAAAGCCACCAGAAGATGAGAATGAGGAGGTTGTGTTACAAAAAACTGTAAAGAAGATCCACTTCGGAAGCTGGGAAGAGAAGGAGAAAGCTGCCATAGAGATCGAGAAACTTGCCCGAGAAGATAAGAAGACTCGGAAGCTCATGGCTGAGCTAGGTGTTATTCAAATTCTTGTTTCTATGGTCGCTTCCGATGTTTCCGGCCATCAGAAAGCTGCCGTCAATGCTCTTATTCAGCTTTCTCATGGAACCTACACGAACAAGGCGCTGATGGTGAACGCCGATATATGCTCAAAGCTTCCCAAAAATGTTGAAGTTCTCGACCAATCAACAAGACATGCCTTTGCCGAGCTTCTGCTTTCATTGTCATCTCTAACCAACACACAATTACCAGTAGCTTCATCACAAATTCTACCGTTCTTAATGGACACGATGAATTCAGATTCAACAGATATGAAGACCAAAGAGATATGTCTAGCCACCATAAGCAATCTCTGCCTTGTGTTGGAAAACGCAGGACCTTTGGTCTTGAACGGGGCAGTGGAGACACTCCTAAGCCTAATGTCAACTAAAGACTTGTCTGAGAAGGCACTAGCGAGTCTAGGGAAATTGGTGGTGACTCAAATGGGTAAAAAAGCAATGGAGGATTGTTTGCTTGTGTCTAAAGGTTTAATAGAGATTCTAACATGGGAAGATATACCGAAATGCCAAGAATACGCGGCGTATATCTTGATGGTATTAGCTCATCAGAGTTGGAGCCAGCGTGAGAAAATGGCCAAGGCAGGGATTGTGCCGGTTCTTCTTGAAGTGAGCTTACTTGGGAGTCCTCTGGTTCAAAAGAGGGCAGTGAAACTTTTGCAATGGTTCAAGGATGAGAGAAATGTACGGATGGGTCCTCATTCGGGTCCACAAACGGGTTGGGTGAGTCCTGGGATGGGATCTCCAATGAGCCCGAGGTCAGGGGAAGAAGGTAGGAAGATGATGAAGAATCTGGTGAAACAGAGTTTATACAAGAACATGGAGATGATAACTCGACGAGGGAATTTGGATATGGAGAGTGAAAGTTGTAGGCTTAAGTCTTTGATCATCAGCACAAGCTCTAAAAGTTTGACTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

438

Amino Acids

48.73

Weight (kDa)

9.08

Isoelectric Point (pI)

51.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ARM_PUB PF25598 93 - 358 2.9e-09 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013290)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27430
fragaria_vesca FvH4_3g07900
malus_domestica MD05G1302200.v1.1 MD10G1280900.v1.1
prunus_persica Prupe.4G060200_v2.0.a1
pyrus_communis pycom05g27980
rosa_chinensis RchiOBHm_Chr5g0010651
rosa_laevigata RLG00000031769
rosa_multiflora Rmu_co8124782.1_g000001 Rmu_sc0006103.1_g000004 Rmu_sc0014635.1_g000002
rosa_roxburghii Rroxscaffold_1G00065720
rosa_rugosa Rorug04G0451500
rosa_samantha Rh5AG083100 Rh5BG078400 Rh5CG091100 Rh5DG078500
rosa_wichuraiana Rw0G000670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 917
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 3 cut(s) 112, 176, 917
AclWI GGATC 2 cut(s) 250, 1144
AcoI YGGCCR 2 cut(s) 400, 972
AcsI RAATTY 4 cut(s) 366, 606, 634, 1240
AcuI CTGAAG 1 cut(s) 18
AfaI GTAC 1 cut(s) 1083
AfiI CCNNNNNNNGG 6 cut(s) 703, 981, 1100, 1112, 1133, 1157
AflII CTTAAG 1 cut(s) 1271
AhdI GACNNNNNGTC 1 cut(s) 722
AjnI CCWGG 1 cut(s) 1126
AjuI GAANNNNNNNTTGG 2 cut(s) 92, 124
Alw26I GTCTC 1 cut(s) 737
AlwI GGATC 2 cut(s) 250, 1144
Ama87I CYCGRG 2 cut(s) 315, 1150
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 2 cut(s) 400, 972
ApeKI GCWGC 2 cut(s) 290, 413
ApoI RAATTY 4 cut(s) 366, 606, 634, 1240
AspLEI GCGC 2 cut(s) 181, 466
AspS9I GGNCC 3 cut(s) 716, 1091, 1103
AsuHPI GGTGA 4 cut(s) 484, 826, 1132, 1204
AvaI CYCGRG 2 cut(s) 315, 1150
AvaII GGWCC 3 cut(s) 716, 1091, 1103
BalI TGGCCA 1 cut(s) 974
BanII GRGCYC 1 cut(s) 1151
BarI GAAGNNNNNNTAC 2 cut(s) 996, 1028
BbsI GAAGAC 2 cut(s) 335, 662
BbvI GCAGC 2 cut(s) 277, 400
BccI CCATC 5 cut(s) 411, 463, 925, 1081, 1126
BceAI ACGGC 1 cut(s) 401
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 1128
BclI TGATCA 1 cut(s) 1281
BcoDI GTCTC 1 cut(s) 737
BfaI CTAG 4 cut(s) 353, 674, 791, 800
BfoI RGCGCY 2 cut(s) 182, 467
BfrI CTTAAG 1 cut(s) 1271
BisI GCNGC 4 cut(s) 177, 291, 414, 918
BlpI GCTNAGC 1 cut(s) 348
BlsI GCNGC 4 cut(s) 178, 292, 415, 919
Bme1390I CCNGG 1 cut(s) 1128
Bme18I GGWCC 3 cut(s) 716, 1091, 1103
BmeRI GACNNNNNGTC 1 cut(s) 722
BmeT110I CYCGRG 2 cut(s) 315, 1150
BmgT120I GGNCC 3 cut(s) 716, 1091, 1103
BmiI GGNNCC 4 cut(s) 448, 956, 1092, 1104
BmrFI CCNGG 1 cut(s) 1128
BmsI GCATC 2 cut(s) 74, 113
BpiI GAAGAC 2 cut(s) 335, 662
Bpu10I CCTNAGC 1 cut(s) 752
Bpu1102I GCTNAGC 1 cut(s) 348
BsaJI CCNNGG 3 cut(s) 975, 1127, 1151
BsaWI WCCGGW 1 cut(s) 184
BsaXI ACNNNNNCTCC 4 cut(s) 218, 248, 946, 976
Bsc4I CCNNNNNNNGG 6 cut(s) 703, 981, 1100, 1112, 1133, 1157
Bse118I RCCGGY 1 cut(s) 991
Bse1I ACTGG 1 cut(s) 590
Bse3DI GCAATG 2 cut(s) 843, 1064
BseAI TCCGGA 1 cut(s) 184
BseBI CCWGG 1 cut(s) 1128
BseDI CCNNGG 3 cut(s) 975, 1127, 1151
BseGI GGATG 4 cut(s) 104, 1075, 1092, 1137
BseLI CCNNNNNNNGG 6 cut(s) 703, 981, 1100, 1112, 1133, 1157
BseMI GCAATG 2 cut(s) 843, 1064
BseMII CTCAG 2 cut(s) 339, 771
BseNI ACTGG 1 cut(s) 590
BseRI GAGGAG 1 cut(s) 239
BseXI GCAGC 2 cut(s) 277, 400
BseYI CCCAGC 1 cut(s) 270
Bsh1236I CGCG 1 cut(s) 917
BshFI GGCC 2 cut(s) 402, 974
BsiHKCI CYCGRG 2 cut(s) 315, 1150
BsiSI CCGG 3 cut(s) 185, 399, 992
BslI CCNNNNNNNGG 6 cut(s) 703, 981, 1100, 1112, 1133, 1157
BsmAI GTCTC 1 cut(s) 737
BsnI GGCC 2 cut(s) 402, 974
BsoBI CYCGRG 2 cut(s) 315, 1150
Bsp1286I GDGCHC 1 cut(s) 1151
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 4 cut(s) 255, 300, 1136, 1281
Bsp1720I GCTNAGC 1 cut(s) 348
BspACI CCGC 3 cut(s) 112, 176, 917
BspANI GGCC 2 cut(s) 402, 974
BspCNI CTCAG 2 cut(s) 340, 772
BspEI TCCGGA 1 cut(s) 184
BspFNI CGCG 1 cut(s) 917
BspLI GGNNCC 4 cut(s) 448, 956, 1092, 1104
BspPI GGATC 2 cut(s) 250, 1144
BspTI CTTAAG 1 cut(s) 1271
BsrDI GCAATG 2 cut(s) 843, 1064
BsrFI RCCGGY 1 cut(s) 991
BsrI ACTGG 1 cut(s) 590
BssAI RCCGGY 1 cut(s) 991
BssECI CCNNGG 3 cut(s) 975, 1127, 1151
BssMI GATC 4 cut(s) 255, 300, 1136, 1281
BssT1I CCWWGG 1 cut(s) 975
Bst2UI CCWGG 1 cut(s) 1128
Bst4CI ACNGT 2 cut(s) 247, 615
Bst6I CTCTTC 2 cut(s) 31, 270
BstAFI CTTAAG 1 cut(s) 1271
BstAPI GCANNNNNTGC 1 cut(s) 693
BstC8I GCNNGC 1 cut(s) 959
BstDEI CTNAG 3 cut(s) 348, 752, 780
BstF5I GGATG 4 cut(s) 104, 1075, 1092, 1137
BstFNI CGCG 1 cut(s) 917
BstH2I RGCGCY 2 cut(s) 182, 467
BstHHI GCGC 2 cut(s) 181, 466
BstKTI GATC 4 cut(s) 258, 303, 1139, 1284
BstMAI GTCTC 1 cut(s) 737
BstMBI GATC 4 cut(s) 255, 300, 1136, 1281
BstMWI GCNNNNNNNGC 2 cut(s) 422, 693
BstNI CCWGG 1 cut(s) 1128
BstNSI RCATGY 1 cut(s) 539
BstSCI CCNGG 1 cut(s) 1126
BstUI CGCG 1 cut(s) 917
BstV1I GCAGC 2 cut(s) 277, 400
BstV2I GAAGAC 2 cut(s) 335, 662
BstX2I RGATCY 2 cut(s) 255, 1136
BstYI RGATCY 2 cut(s) 255, 1136
BsuRI GGCC 2 cut(s) 402, 974
BtsCI GGATG 4 cut(s) 104, 1075, 1092, 1137
BtsI GCAGTG 2 cut(s) 744, 1050
BtsIMutI CAGTG 2 cut(s) 744, 1050
Cac8I GCNNGC 1 cut(s) 959
CfoI GCGC 2 cut(s) 181, 466
Cfr10I RCCGGY 1 cut(s) 991
Cfr13I GGNCC 3 cut(s) 716, 1091, 1103
Csp6I GTAC 1 cut(s) 1082
CviAII CATG 6 cut(s) 55, 343, 443, 536, 885, 1216
CviQI GTAC 1 cut(s) 1082
DdeI CTNAG 3 cut(s) 348, 752, 780
DpnI GATC 4 cut(s) 257, 302, 1138, 1283
DpnII GATC 4 cut(s) 255, 300, 1136, 1281
DriI GACNNNNNGTC 1 cut(s) 722
EaeI YGGCCR 2 cut(s) 400, 972
Eam1104I CTCTTC 2 cut(s) 31, 270
Eam1105I GACNNNNNGTC 1 cut(s) 722
EarI CTCTTC 2 cut(s) 31, 270
Eco130I CCWWGG 1 cut(s) 975
Eco24I GRGCYC 1 cut(s) 1151
Eco47I GGWCC 3 cut(s) 716, 1091, 1103
Eco57I CTGAAG 1 cut(s) 18
Eco88I CYCGRG 2 cut(s) 315, 1150
EcoO109I RGGNCCY 2 cut(s) 716, 1091
EcoRI GAATTC 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 1126
EcoT14I CCWWGG 1 cut(s) 975
EcoT38I GRGCYC 1 cut(s) 1151
ErhI CCWWGG 1 cut(s) 975
FaeI CATG 6 cut(s) 58, 346, 446, 539, 888, 1219
FalI AAGNNNNNCTT 5 cut(s) 50, 82, 245, 277, 1294
FatI CATG 6 cut(s) 54, 342, 442, 535, 884, 1215
FbaI TGATCA 1 cut(s) 1281
Fnu4HI GCNGC 4 cut(s) 177, 291, 414, 918
FokI GGATG 4 cut(s) 91, 1082, 1099, 1144
FriOI GRGCYC 1 cut(s) 1151
Fsp4HI GCNGC 4 cut(s) 177, 291, 414, 918
FspBI CTAG 4 cut(s) 353, 674, 791, 800
GlaI GCGC 2 cut(s) 180, 465
GluI GCNGC 4 cut(s) 177, 291, 414, 918
GsaI CCCAGC 1 cut(s) 274
HaeII RGCGCY 2 cut(s) 182, 467
HaeIII GGCC 2 cut(s) 402, 974
HapII CCGG 3 cut(s) 185, 399, 992
HhaI GCGC 2 cut(s) 181, 466
Hin1II CATG 6 cut(s) 58, 346, 446, 539, 888, 1219
Hin6I GCGC 2 cut(s) 179, 464
HinP1I GCGC 2 cut(s) 179, 464
HincII GTYRAC 1 cut(s) 765
HindII GTYRAC 1 cut(s) 765
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 9 cut(s) 79, 331, 641, 796, 817, 877, 1021, 1123, 1186
HpaII CCGG 3 cut(s) 185, 399, 992
HphI GGTGA 4 cut(s) 484, 826, 1132, 1204
Hpy166II GTNNAC 3 cut(s) 475, 765, 1106
Hpy188I TCNGA 7 cut(s) 266, 336, 391, 408, 640, 781, 948
Hpy8I GTNNAC 3 cut(s) 475, 765, 1106
Hpy99I CGWCG 1 cut(s) 1236
HpyAV CCTTC 5 cut(s) 118, 163, 274, 778, 1160
HpyCH4III ACNGT 2 cut(s) 247, 615
HpyCH4V TGCA 1 cut(s) 1057
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 693
HpyF3I CTNAG 3 cut(s) 348, 752, 780
Hsp92II CATG 6 cut(s) 58, 346, 446, 539, 888, 1219
HspAI GCGC 2 cut(s) 179, 464
Kpn2I TCCGGA 1 cut(s) 184
Ksp22I TGATCA 1 cut(s) 1281
Kzo9I GATC 4 cut(s) 255, 300, 1136, 1281
LmnI GCTCC 1 cut(s) 954
Lsp1109I GCAGC 2 cut(s) 277, 400
LweI GCATC 2 cut(s) 74, 113
MaeI CTAG 4 cut(s) 353, 674, 791, 800
MaeIII GTNAC 3 cut(s) 132, 234, 814
MalI GATC 4 cut(s) 257, 302, 1138, 1283
MboI GATC 4 cut(s) 255, 300, 1136, 1281
MflI RGATCY 2 cut(s) 255, 1136
MhlI GDGCHC 1 cut(s) 1151
MlsI TGGCCA 1 cut(s) 974
MluCI AATT 7 cut(s) 192, 366, 584, 606, 634, 806, 1240
MluNI TGGCCA 1 cut(s) 974
MlyI GAGTC 5 cut(s) 325, 805, 811, 1030, 1132
MmeI TCCRAC 2 cut(s) 684, 932
Mox20I TGGCCA 1 cut(s) 974
MroI TCCGGA 1 cut(s) 184
MscI TGGCCA 1 cut(s) 974
MseI TTAA 3 cut(s) 620, 869, 1272
MslI CAYNNNNRTG 1 cut(s) 1220
Msp20I TGGCCA 1 cut(s) 974
MspCI CTTAAG 1 cut(s) 1271
MspI CCGG 3 cut(s) 185, 399, 992
MspR9I CCNGG 1 cut(s) 1128
MvaI CCWGG 1 cut(s) 1128
MvnI CGCG 1 cut(s) 917
MwoI GCNNNNNNNGC 2 cut(s) 422, 693
NdeII GATC 4 cut(s) 255, 300, 1136, 1281
NlaIII CATG 6 cut(s) 58, 346, 446, 539, 888, 1219
NlaIV GGNNCC 4 cut(s) 448, 956, 1092, 1104
NmeAIII GCCGAG 1 cut(s) 571
NmuCI GTSAC 1 cut(s) 814
NspI RCATGY 1 cut(s) 539
PfeI GAWTC 4 cut(s) 79, 641, 877, 1186
PkrI GCNGC 4 cut(s) 178, 292, 415, 919
PleI GAGTC 5 cut(s) 325, 804, 811, 1029, 1131
PpsI GAGTC 5 cut(s) 325, 804, 811, 1029, 1131
PpuMI RGGWCCY 2 cut(s) 716, 1091
Psp5II RGGWCCY 2 cut(s) 716, 1091
Psp6I CCWGG 1 cut(s) 1126
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 1 cut(s) 1126
PspN4I GGNNCC 4 cut(s) 448, 956, 1092, 1104
PspPI GGNCC 3 cut(s) 716, 1091, 1103
PspPPI RGGWCCY 2 cut(s) 716, 1091
PsuI RGATCY 2 cut(s) 255, 1136
RsaI GTAC 1 cut(s) 1083
RsaNI GTAC 1 cut(s) 1082
RseI CAYNNNNRTG 1 cut(s) 1220
SaqAI TTAA 3 cut(s) 620, 869, 1272
SatI GCNGC 4 cut(s) 177, 291, 414, 918
Sau3AI GATC 4 cut(s) 255, 300, 1136, 1281
Sau96I GGNCC 3 cut(s) 716, 1091, 1103
SchI GAGTC 5 cut(s) 325, 805, 811, 1030, 1132
ScrFI CCNGG 1 cut(s) 1128
SduI GDGCHC 1 cut(s) 1151
SfaNI GCATC 2 cut(s) 74, 113
SinI GGWCC 3 cut(s) 716, 1091, 1103
SmiMI CAYNNNNRTG 1 cut(s) 1220
SmlI CTYRAG 1 cut(s) 1271
SmoI CTYRAG 1 cut(s) 1271
Sse9I AATT 7 cut(s) 192, 366, 584, 606, 634, 806, 1240
SsiI CCGC 3 cut(s) 112, 176, 917
SspMI CTAG 4 cut(s) 353, 674, 791, 800
StyD4I CCNGG 1 cut(s) 1126
StyI CCWWGG 1 cut(s) 975
TaaI ACNGT 2 cut(s) 247, 615
TaqI TCGA 6 cut(s) 13, 82, 165, 303, 519, 1231
TasI AATT 7 cut(s) 192, 366, 584, 606, 634, 806, 1240
TauI GCSGC 2 cut(s) 179, 920
TfiI GAWTC 4 cut(s) 79, 641, 877, 1186
Tru1I TTAA 3 cut(s) 620, 869, 1272
Tru9I TTAA 3 cut(s) 620, 869, 1272
TscAI CASTG 2 cut(s) 744, 1050
TseFI GTSAC 1 cut(s) 814
TseI GCWGC 2 cut(s) 290, 413
Tsp45I GTSAC 1 cut(s) 814
TspDTI ATGAA 6 cut(s) 71, 549, 588, 647, 668, 1196
TspGWI ACGGA 1 cut(s) 1099
TspRI CASTG 2 cut(s) 744, 1050
Vha464I CTTAAG 1 cut(s) 1271
VpaK11BI GGWCC 3 cut(s) 716, 1091, 1103
XapI RAATTY 4 cut(s) 366, 606, 634, 1240
XceI RCATGY 1 cut(s) 539
XcmI CCANNNNNNNNNTGG 1 cut(s) 1113
XspI CTAG 4 cut(s) 353, 674, 791, 800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.