AT2G27500

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007295
Physical Location & Seq
Reverse (-)
11752064 .. 11754066
2003 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G27500.1

Sequence Viewer

Length: 1179 bp
ATGGCCACACATAGTCTCTCCTTCTTCTTCAGAGTTCTTCTCCTCCTCTTTCTTACTCTCTCAGAGAGAATTAAAGGGCAAGGTGTGGGAATCAACTACGGCCAAATAGCAAACAACCTCCCATCTCCGGCAAGAGTCGCCGTGCTCCTTCGATCACTAAACATCACAAGAGTAAAACTCTACGACGCAGATCCAAACGTCCTCTTCTCCTTCTCAAACTCACAAGTAGATTTCATGATCGGTTTAGGCAACGAGTATCTTCAAAACATGTCAACAGATCCAACCAAAGCTCAAGATTGGTTACAACAAAGACTTGAACCACATATCTCAAAAACACGAATAACCTCAATAGTTGTCGGCAACGAAATCTTCAAAACCAATGATCATGTCCTCATCCAAAGCCTATTACCGGCTATGAAATCGGTTTACGCTGCTTTAACCAATCTTGGTTTAGAGAAACAAGTAACCGTAACTTCAGCTCATTCTTTAGACATTCTTTCAACATCTTATCCTCCTTCTTCAGGATCATTCAAAGAAGAGTTTATTCAATATCTTCAACCACTTCTTGATTTTCATTCTCAAATCGAATCACCTTTCTTGATAAATGCTTATCCCTTTTTTGCTTATAAAGATAGTCCTAAAGAAGTTCCATTAGAGTATGTTTTGTTTCAACCTAATCAAGGTATGGTTGATCCAAACACTAATCTTCATTATGACAACATGTTGTTTGCTCAAGTTGATGCGCTTTACTCTGCCATTAAGACTTTGGGACATACCGATATCGAGGTTCGGATATCGGAAACCGGTTGGCCTTCTAAAGGAGATGAGAATGAGATTGGAGCTTCGCCGGAGAACGCGGCTTTGTATAATGGGAATTTGTTGAAGTTGATTCAGCAGAGGAAAGGAACTCCGGCGAAGCAATCTGTTCCTATTGATGTTTATGTGTTTGCTCTGTTTAATGAGAATCTTAAACCGGGTCCGGTTTCTGAGAGGAATTATGGACTTTTTTATCCGGATGGTAAACCGGTTTACAATGTCGGTATGCAAGGTTATTTACCTGATATTATCTATACTTCAAGGGCAACTACAATTAAGATTTTGAATTTGTGGAGAGTCGTGATGGGTTTGGCTGTGGCTTGGTTCATACTCGATATGGGCGACAAGATGAGGATGAGATAA

Protein Analysis

392

Amino Acids

44.07

Weight (kDa)

6.18

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 29 - 346 1e-82 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 627
AccII CGCG 1 cut(s) 857
AccIII TCCGGA 1 cut(s) 1012
AciI CCGC 1 cut(s) 857
AclWI GGATC 4 cut(s) 185, 272, 532, 686
AcoI YGGCCR 2 cut(s) 3, 100
AcsI RAATTY 2 cut(s) 874, 1102
AcuI CTGAAG 3 cut(s) 13, 459, 504
AfiI CCNNNNNNNGG 5 cut(s) 127, 409, 521, 680, 818
AflIII ACRYGT 2 cut(s) 267, 720
AgeI ACCGGT 2 cut(s) 803, 1024
AluBI AGCT 3 cut(s) 290, 479, 842
AluI AGCT 3 cut(s) 290, 479, 842
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 4 cut(s) 185, 272, 532, 686
Aor13HI TCCGGA 1 cut(s) 1012
AoxI GGCC 3 cut(s) 3, 100, 809
ApeKI GCWGC 1 cut(s) 431
ApoI RAATTY 2 cut(s) 874, 1102
AsiGI ACCGGT 2 cut(s) 803, 1024
AspLEI GCGC 1 cut(s) 745
AspS9I GGNCC 1 cut(s) 977
AsuC2I CCSGG 1 cut(s) 975
AsuHPI GGTGA 1 cut(s) 582
AvaII GGWCC 1 cut(s) 977
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 1 cut(s) 418
BccI CCATC 3 cut(s) 130, 1010, 1114
BceAI ACGGC 2 cut(s) 115, 125
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 975
BcoDI GTCTC 1 cut(s) 20
BisI GCNGC 2 cut(s) 432, 858
BlsI GCNGC 2 cut(s) 433, 859
Bme1390I CCNGG 1 cut(s) 975
Bme18I GGWCC 1 cut(s) 977
BmgT120I GGNCC 1 cut(s) 977
BmiI GGNNCC 1 cut(s) 978
BmrFI CCNGG 1 cut(s) 975
BmsI GCATC 1 cut(s) 730
BplI GAGNNNNNCTC 4 cut(s) 24, 56, 162, 194
BpuEI CTTGAG 2 cut(s) 276, 717
BpuMI CCSGG 1 cut(s) 975
BsaWI WCCGGW 4 cut(s) 803, 979, 1012, 1024
Bsc4I CCNNNNNNNGG 5 cut(s) 127, 409, 521, 680, 818
Bse118I RCCGGY 3 cut(s) 409, 803, 1024
BseAI TCCGGA 1 cut(s) 1012
BseGI GGATG 3 cut(s) 393, 1021, 1176
BseLI CCNNNNNNNGG 5 cut(s) 127, 409, 521, 680, 818
BseMII CTCAG 2 cut(s) 75, 978
BseRI GAGGAG 2 cut(s) 32, 35
BseXI GCAGC 1 cut(s) 418
Bsh1236I CGCG 1 cut(s) 857
BshFI GGCC 3 cut(s) 5, 102, 811
BshTI ACCGGT 2 cut(s) 803, 1024
BsiHKAI GWGCWC 1 cut(s) 147
BsiSI CCGG 9 cut(s) 128, 410, 804, 848, 911, 974, 980, 1013, 1025
BslFI GGGAC 1 cut(s) 783
BslI CCNNNNNNNGG 5 cut(s) 127, 409, 521, 680, 818
BsmAI GTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 783
BsnI GGCC 3 cut(s) 5, 102, 811
Bsp1286I GDGCHC 1 cut(s) 147
Bsp13I TCCGGA 1 cut(s) 1012
Bsp143I GATC 7 cut(s) 152, 190, 237, 277, 382, 524, 691
BspACI CCGC 1 cut(s) 857
BspANI GGCC 3 cut(s) 5, 102, 811
BspCNI CTCAG 2 cut(s) 74, 979
BspEI TCCGGA 1 cut(s) 1012
BspFNI CGCG 1 cut(s) 857
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 978
BspPI GGATC 4 cut(s) 185, 272, 532, 686
BsrFI RCCGGY 3 cut(s) 409, 803, 1024
BssAI RCCGGY 3 cut(s) 409, 803, 1024
BssMI GATC 7 cut(s) 152, 190, 237, 277, 382, 524, 691
Bst4CI ACNGT 1 cut(s) 469
Bst6I CTCTTC 2 cut(s) 209, 531
BstDEI CTNAG 2 cut(s) 61, 987
BstENI CCTNNNNNAGG 3 cut(s) 519, 678, 816
BstF5I GGATG 3 cut(s) 393, 1021, 1176
BstFNI CGCG 1 cut(s) 857
BstHHI GCGC 1 cut(s) 745
BstKTI GATC 7 cut(s) 155, 193, 240, 280, 385, 527, 694
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 7 cut(s) 152, 190, 237, 277, 382, 524, 691
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstNSI RCATGY 2 cut(s) 271, 724
BstSCI CCNGG 1 cut(s) 973
BstUI CGCG 1 cut(s) 857
BstV1I GCAGC 1 cut(s) 418
BstX2I RGATCY 2 cut(s) 190, 277
BstYI RGATCY 2 cut(s) 190, 277
BsuRI GGCC 3 cut(s) 5, 102, 811
BtsCI GGATG 3 cut(s) 393, 1021, 1176
CciI TCATGA 1 cut(s) 234
CfoI GCGC 1 cut(s) 745
Cfr10I RCCGGY 3 cut(s) 409, 803, 1024
Cfr13I GGNCC 1 cut(s) 977
CseI GACGC 1 cut(s) 194
CspAI ACCGGT 2 cut(s) 803, 1024
CviAII CATG 4 cut(s) 235, 268, 386, 721
DdeI CTNAG 2 cut(s) 61, 987
DpnI GATC 7 cut(s) 154, 192, 239, 279, 384, 526, 693
DpnII GATC 7 cut(s) 152, 190, 237, 277, 382, 524, 691
EaeI YGGCCR 2 cut(s) 3, 100
Eam1104I CTCTTC 2 cut(s) 209, 531
EarI CTCTTC 2 cut(s) 209, 531
Eco32I GATATC 2 cut(s) 781, 795
Eco47I GGWCC 1 cut(s) 977
Eco57I CTGAAG 3 cut(s) 13, 459, 504
EcoNI CCTNNNNNAGG 3 cut(s) 519, 678, 816
EcoRV GATATC 2 cut(s) 781, 795
FaeI CATG 4 cut(s) 238, 271, 389, 724
FaqI GGGAC 1 cut(s) 783
FatI CATG 4 cut(s) 234, 267, 385, 720
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 2 cut(s) 432, 858
FokI GGATG 2 cut(s) 380, 1028
Fsp4HI GCNGC 2 cut(s) 432, 858
GlaI GCGC 1 cut(s) 744
GluI GCNGC 2 cut(s) 432, 858
HaeIII GGCC 3 cut(s) 5, 102, 811
HapII CCGG 9 cut(s) 128, 410, 804, 848, 911, 974, 980, 1013, 1025
HgaI GACGC 1 cut(s) 194
HhaI GCGC 1 cut(s) 745
Hin1II CATG 4 cut(s) 238, 271, 389, 724
Hin6I GCGC 1 cut(s) 743
HinP1I GCGC 1 cut(s) 743
HincII GTYRAC 1 cut(s) 273
HindII GTYRAC 1 cut(s) 273
HinfI GANTC 6 cut(s) 90, 135, 587, 889, 964, 1113
HpaII CCGG 9 cut(s) 128, 410, 804, 848, 911, 974, 980, 1013, 1025
HphI GGTGA 1 cut(s) 582
Hpy166II GTNNAC 4 cut(s) 273, 427, 1022, 1030
Hpy188I TCNGA 5 cut(s) 32, 64, 792, 799, 988
Hpy188III TCNNGA 7 cut(s) 235, 293, 522, 566, 598, 1013, 1117
Hpy8I GTNNAC 4 cut(s) 273, 427, 1022, 1030
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 5 cut(s) 31, 158, 220, 525, 822
HpyCH4III ACNGT 1 cut(s) 469
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 1 cut(s) 1045
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 2 cut(s) 61, 987
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 4 cut(s) 238, 271, 389, 724
HspAI GCGC 1 cut(s) 743
Kpn2I TCCGGA 1 cut(s) 1012
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 7 cut(s) 152, 190, 237, 277, 382, 524, 691
LmnI GCTCC 2 cut(s) 150, 839
Lsp1109I GCAGC 1 cut(s) 418
LweI GCATC 1 cut(s) 730
MaeII ACGT 1 cut(s) 198
MaeIII GTNAC 3 cut(s) 300, 463, 469
MalI GATC 7 cut(s) 154, 192, 239, 279, 384, 526, 693
MboI GATC 7 cut(s) 152, 190, 237, 277, 382, 524, 691
MflI RGATCY 2 cut(s) 190, 277
MhlI GDGCHC 1 cut(s) 147
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 69, 874, 994, 1089, 1102
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 144, 1122
MmeI TCCRAC 1 cut(s) 305
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 1012
MscI TGGCCA 1 cut(s) 5
MseI TTAA 6 cut(s) 72, 437, 759, 957, 969, 1092
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 9 cut(s) 128, 410, 804, 848, 911, 974, 980, 1013, 1025
MspR9I CCNGG 1 cut(s) 975
MvnI CGCG 1 cut(s) 857
MwoI GCNNNNNNNGC 1 cut(s) 137
NciI CCSGG 1 cut(s) 975
NdeII GATC 7 cut(s) 152, 190, 237, 277, 382, 524, 691
NlaIII CATG 4 cut(s) 238, 271, 389, 724
NlaIV GGNNCC 1 cut(s) 978
NspI RCATGY 2 cut(s) 271, 724
PagI TCATGA 1 cut(s) 234
PciI ACATGT 2 cut(s) 267, 720
PcsI WCGNNNNNNNCGW 1 cut(s) 1155
PfeI GAWTC 4 cut(s) 90, 587, 889, 964
PinAI ACCGGT 2 cut(s) 803, 1024
PkrI GCNGC 2 cut(s) 433, 859
PleI GAGTC 2 cut(s) 143, 1121
PpsI GAGTC 2 cut(s) 143, 1121
PscI ACATGT 2 cut(s) 267, 720
PsiI TTATAA 1 cut(s) 627
PspN4I GGNNCC 1 cut(s) 978
PspPI GGNCC 1 cut(s) 977
PsuI RGATCY 2 cut(s) 190, 277
SaqAI TTAA 6 cut(s) 72, 437, 759, 957, 969, 1092
SatI GCNGC 2 cut(s) 432, 858
Sau3AI GATC 7 cut(s) 152, 190, 237, 277, 382, 524, 691
Sau96I GGNCC 1 cut(s) 977
SchI GAGTC 2 cut(s) 144, 1122
ScrFI CCNGG 1 cut(s) 975
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 1 cut(s) 730
SinI GGWCC 1 cut(s) 977
SmlI CTYRAG 2 cut(s) 291, 732
SmoI CTYRAG 2 cut(s) 291, 732
Sse9I AATT 5 cut(s) 69, 874, 994, 1089, 1102
SsiI CCGC 1 cut(s) 857
StyD4I CCNGG 1 cut(s) 973
TaaI ACNGT 1 cut(s) 469
TaiI ACGT 1 cut(s) 201
TaqI TCGA 4 cut(s) 151, 585, 783, 1149
TasI AATT 5 cut(s) 69, 874, 994, 1089, 1102
TauI GCSGC 1 cut(s) 860
TfiI GAWTC 4 cut(s) 90, 587, 889, 964
Tru1I TTAA 6 cut(s) 72, 437, 759, 957, 969, 1092
Tru9I TTAA 6 cut(s) 72, 437, 759, 957, 969, 1092
TseI GCWGC 1 cut(s) 431
TspDTI ATGAA 5 cut(s) 223, 431, 563, 698, 1132
VpaK11BI GGWCC 1 cut(s) 977
XagI CCTNNNNNAGG 3 cut(s) 519, 678, 816
XapI RAATTY 2 cut(s) 874, 1102
XceI RCATGY 2 cut(s) 271, 724
XcmI CCANNNNNNNNNTGG 1 cut(s) 763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.