Rh5AG300400

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0007295
Physical Location & Seq
Forward (+)
42128831 .. 42131462
2632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG300400.1

Sequence Viewer

Length: 1176 bp
ATGTTTGATCTGTTTATGCATAAAATTTCTGACTCTGTTTTTAACAATGTTTCAGATTCAGCTCTGTTCCATGTGCAGTGCTTAAGCTTCGGCATCAACTACGGCCAAATCGCCAACAACCTCCCTCCTCCTTCCCAAGTAGCGGGGCTCCTCCAGTCCCTCAACGTCAGCAGAGTCAAACTCTACGACGCCGATCCCAATGTCCTCCAAGCCTTCTCCAACTCCGAGGTCGACTTCATCATCGCACTCGGAAACGAGCTCCTACCCAACATGACCGAACCGCTCAAGGCCCAGAATTGGATCCAACAACACGTCACCCCTCACCTCCCGCAGACCAAAATCTCCTCCATCCTCGTCGGCAACGAGATCCTCGGCGGAACCGACACCCGTCTCATGTCGTACCTCCTCATCGCAATGCAATCCGTCTACGCCGCCCTCGTCAACCTCGGACTTTCCAAAACCATCGTCGTCACCAGCGCGCATTCCCTTAACATTTTGGGAAACTCCTACCCGCCGTCGGCCGGGACTTTCCGGGAAGACCTCGCCCAATACATCAAGCCAATTCTAGACTTCCACTCGCAGACCAATGCCCCGTTCCTCGTAAACGTGTACCCTTACTTTGCTTACAAGGGCAGTCCTGATGAAGTTTCCTTGGAGTACGTGCTTTTTCAGCCAAACTCCGGCATGAATGATTCCGGCACCAATCTTCACTACGATAACATGCTCGATGCTCAGATTGACGCTGTCTATGCCGCAATGAAAGTGATGGGCCACACTGATATCGACGTTCGGATATCTGAAACCGGCTGGCCGTCCAAGGGAGACGAGGATGAAGCCGGAGCCACGCCGGAGAATGCCGGGATTTACAATGGGAATTTGCTCAGGAAAATTGTGGAGAAGAAAGGCACTCCGGCCAAGCCTGACGTTCCTATTGATACGTATGTGTTTGCTCTGTTTAATGAGGACATGAAGCCCGGTCCGGCGTCGGAGAGGAACTACGGGCTGTTTTATCCCAATGGGACTCGGGTGTATGATATGGGAGATGTCGGAGCTGCCGGACCTGACGGAGCAGATGGTTATCTTCCCCAGTTTACTTTTTCTTCAGCTCATGATATTAATGCTTTGTCGATCTTCAATTTTCTGATATTTCTTATAGTGTACTTATTATGGGAATAG

Protein Analysis

391

Amino Acids

42.97

Weight (kDa)

4.66

Isoelectric Point (pI)

33.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 31 - 346 1.1e-85 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 698
AccBSI CCGCTC 1 cut(s) 283
AccI GTMKAC 2 cut(s) 231, 426
AccII CGCG 1 cut(s) 479
AciI CCGC 7 cut(s) 143, 281, 329, 375, 432, 512, 753
AclWI GGATC 4 cut(s) 188, 295, 308, 361
AcoI YGGCCR 4 cut(s) 103, 519, 809, 912
AcsI RAATTY 2 cut(s) 24, 874
AcuI CTGAAG 1 cut(s) 1086
AcyI GRCGYC 2 cut(s) 189, 983
AfaI GTAC 4 cut(s) 401, 611, 659, 1160
AfiI CCNNNNNNNGG 6 cut(s) 142, 297, 517, 521, 680, 818
AflII CTTAAG 1 cut(s) 82
AflIII ACRYGT 2 cut(s) 310, 606
AgsI TTSAA 1 cut(s) 1135
AjiI CACGTC 1 cut(s) 313
AjuI GAANNNNNNNTTGG 2 cut(s) 578, 610
AluBI AGCT 5 cut(s) 62, 87, 259, 1052, 1106
AluI AGCT 5 cut(s) 62, 87, 259, 1052, 1106
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 395, 816
AlwI GGATC 4 cut(s) 188, 295, 308, 361
Ama87I CYCGRG 1 cut(s) 1023
AoxI GGCC 6 cut(s) 103, 288, 519, 769, 809, 912
ApeKI GCWGC 1 cut(s) 1052
ApoI RAATTY 2 cut(s) 24, 874
ArsI GACNNNNNNTTYG 2 cut(s) 450, 482
AseI ATTAAT 1 cut(s) 1116
AspLEI GCGC 2 cut(s) 479, 481
AspS9I GGNCC 4 cut(s) 289, 769, 977, 1058
AsuC2I CCSGG 4 cut(s) 523, 533, 859, 975
AsuHPI GGTGA 3 cut(s) 307, 314, 463
AvaI CYCGRG 1 cut(s) 1023
AvaII GGWCC 2 cut(s) 977, 1058
BaeI ACNNNNGTAYC 2 cut(s) 927, 960
BamHI GGATCC 1 cut(s) 300
BanI GGYRCC 1 cut(s) 698
BanII GRGCYC 2 cut(s) 150, 261
BbsI GAAGAC 1 cut(s) 543
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 1039
BccI CCATC 4 cut(s) 356, 470, 760, 1067
BceAI ACGGC 3 cut(s) 118, 499, 796
BcnI CCSGG 4 cut(s) 523, 533, 859, 975
BcoDI GTCTC 2 cut(s) 395, 816
BfaI CTAG 1 cut(s) 566
BfrI CTTAAG 1 cut(s) 82
BisI GCNGC 3 cut(s) 432, 753, 1053
BlsI GCNGC 3 cut(s) 433, 754, 1054
Bme1390I CCNGG 4 cut(s) 523, 533, 859, 975
Bme18I GGWCC 2 cut(s) 977, 1058
BmeT110I CYCGRG 1 cut(s) 1023
BmgBI CACGTC 1 cut(s) 313
BmgT120I GGNCC 4 cut(s) 289, 769, 977, 1058
BmiI GGNNCC 5 cut(s) 149, 302, 379, 700, 841
BmrFI CCNGG 4 cut(s) 523, 533, 859, 975
BmrI ACTGGG 1 cut(s) 1081
BmsI GCATC 2 cut(s) 102, 718
BmuI ACTGGG 1 cut(s) 1081
BoxI GACNNNNGTC 1 cut(s) 387
BpiI GAAGAC 1 cut(s) 543
BplI GAGNNNNNCTC 2 cut(s) 165, 197
BpmI CTGGAG 1 cut(s) 137
Bpu10I CCTNAGC 1 cut(s) 881
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 4 cut(s) 523, 533, 859, 975
BsaAI YACGTR 2 cut(s) 661, 939
BsaBI GATNNNNATC 1 cut(s) 1077
BsaHI GRCGYC 2 cut(s) 189, 983
BsaJI CCNNGG 5 cut(s) 225, 370, 445, 651, 816
Bsc4I CCNNNNNNNGG 6 cut(s) 142, 297, 517, 521, 680, 818
Bse118I RCCGGY 1 cut(s) 803
Bse1I ACTGG 2 cut(s) 154, 1087
Bse3DI GCAATG 2 cut(s) 420, 762
Bse8I GATNNNNATC 1 cut(s) 1077
BseDI CCNNGG 5 cut(s) 225, 370, 445, 651, 816
BseGI GGATG 2 cut(s) 348, 835
BseJI GATNNNNATC 1 cut(s) 1077
BseLI CCNNNNNNNGG 6 cut(s) 142, 297, 517, 521, 680, 818
BseMI GCAATG 2 cut(s) 420, 762
BseMII CTCAG 2 cut(s) 746, 895
BseNI ACTGG 2 cut(s) 154, 1087
BsePI GCGCGC 1 cut(s) 477
BseRI GAGGAG 4 cut(s) 117, 140, 334, 395
BseX3I CGGCCG 1 cut(s) 519
BseXI GCAGC 1 cut(s) 1039
BsgI GTGCAG 1 cut(s) 95
Bsh1236I CGCG 1 cut(s) 479
Bsh1285I CGRYCG 1 cut(s) 522
BshFI GGCC 6 cut(s) 105, 290, 521, 771, 811, 914
BshNI GGYRCC 1 cut(s) 698
BsiEI CGRYCG 1 cut(s) 522
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 1023
BslFI GGGAC 3 cut(s) 142, 538, 1033
BslI CCNNNNNNNGG 6 cut(s) 142, 297, 517, 521, 680, 818
BsmAI GTCTC 2 cut(s) 395, 816
BsmBI CGTCTC 2 cut(s) 395, 816
BsmFI GGGAC 3 cut(s) 142, 538, 1033
BsmI GAATGC 2 cut(s) 481, 859
BsnI GGCC 6 cut(s) 105, 290, 521, 771, 811, 914
BsoBI CYCGRG 1 cut(s) 1023
Bsp1286I GDGCHC 2 cut(s) 150, 261
Bsp143I GATC 5 cut(s) 7, 193, 300, 366, 1128
BspACI CCGC 7 cut(s) 143, 281, 329, 375, 432, 512, 753
BspANI GGCC 6 cut(s) 105, 290, 521, 771, 811, 914
BspCNI CTCAG 2 cut(s) 745, 894
BspFNI CGCG 1 cut(s) 479
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 5 cut(s) 149, 302, 379, 700, 841
BspPI GGATC 4 cut(s) 188, 295, 308, 361
BspT107I GGYRCC 1 cut(s) 698
BspTI CTTAAG 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 283
BsrDI GCAATG 2 cut(s) 420, 762
BsrFI RCCGGY 1 cut(s) 803
BsrI ACTGG 2 cut(s) 154, 1087
BssAI RCCGGY 1 cut(s) 803
BssECI CCNNGG 5 cut(s) 225, 370, 445, 651, 816
BssHII GCGCGC 1 cut(s) 477
BssMI GATC 5 cut(s) 7, 193, 300, 366, 1128
BssNI GRCGYC 2 cut(s) 189, 983
BssT1I CCWWGG 2 cut(s) 651, 816
BstACI GRCGYC 2 cut(s) 189, 983
BstAFI CTTAAG 1 cut(s) 82
BstBAI YACGTR 2 cut(s) 661, 939
BstC8I GCNNGC 2 cut(s) 479, 809
BstDEI CTNAG 2 cut(s) 732, 881
BstF5I GGATG 2 cut(s) 348, 835
BstFNI CGCG 1 cut(s) 479
BstHHI GCGC 2 cut(s) 479, 481
BstKTI GATC 5 cut(s) 10, 196, 303, 369, 1131
BstMAI GTCTC 2 cut(s) 395, 816
BstMBI GATC 5 cut(s) 7, 193, 300, 366, 1128
BstMCI CGRYCG 1 cut(s) 522
BstMWI GCNNNNNNNGC 2 cut(s) 670, 749
BstNSI RCATGY 1 cut(s) 724
BstPAI GACNNNNGTC 1 cut(s) 387
BstSCI CCNGG 4 cut(s) 521, 531, 857, 973
BstSNI TACGTA 1 cut(s) 939
BstUI CGCG 1 cut(s) 479
BstV1I GCAGC 1 cut(s) 1039
BstV2I GAAGAC 1 cut(s) 543
BstX2I RGATCY 2 cut(s) 300, 366
BstYI RGATCY 2 cut(s) 300, 366
BstZI CGGCCG 1 cut(s) 519
BsuRI GGCC 6 cut(s) 105, 290, 521, 771, 811, 914
BtgZI GCGATG 2 cut(s) 226, 394
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 2 cut(s) 348, 835
BtsI GCAGTG 1 cut(s) 83
BtsIMutI CAGTG 2 cut(s) 83, 774
Cac8I GCNNGC 2 cut(s) 479, 809
CciI TCATGA 1 cut(s) 1108
CfoI GCGC 2 cut(s) 479, 481
Cfr10I RCCGGY 1 cut(s) 803
Cfr13I GGNCC 4 cut(s) 289, 769, 977, 1058
CpoI CGGWCCG 1 cut(s) 977
CseI GACGC 3 cut(s) 197, 749, 972
Csp6I GTAC 4 cut(s) 400, 610, 658, 1159
CspI CGGWCCG 1 cut(s) 977
CviAII CATG 7 cut(s) 71, 271, 394, 685, 721, 967, 1109
CviQI GTAC 4 cut(s) 400, 610, 658, 1159
DdeI CTNAG 2 cut(s) 732, 881
DpnI GATC 5 cut(s) 9, 195, 302, 368, 1130
DpnII GATC 5 cut(s) 7, 193, 300, 366, 1128
EaeI YGGCCR 4 cut(s) 103, 519, 809, 912
EagI CGGCCG 1 cut(s) 519
EciI GGCGGA 1 cut(s) 390
Ecl136II GAGCTC 1 cut(s) 259
EclXI CGGCCG 1 cut(s) 519
Eco105I TACGTA 1 cut(s) 939
Eco130I CCWWGG 2 cut(s) 651, 816
Eco24I GRGCYC 2 cut(s) 150, 261
Eco32I GATATC 2 cut(s) 781, 795
Eco47I GGWCC 2 cut(s) 977, 1058
Eco52I CGGCCG 1 cut(s) 519
Eco53kI GAGCTC 1 cut(s) 259
Eco57I CTGAAG 1 cut(s) 1086
Eco88I CYCGRG 1 cut(s) 1023
EcoICRI GAGCTC 1 cut(s) 259
EcoRV GATATC 2 cut(s) 781, 795
EcoT14I CCWWGG 2 cut(s) 651, 816
EcoT22I ATGCAT 1 cut(s) 21
EcoT38I GRGCYC 2 cut(s) 150, 261
ErhI CCWWGG 2 cut(s) 651, 816
Esp3I CGTCTC 2 cut(s) 395, 816
FaeI CATG 7 cut(s) 74, 274, 397, 688, 724, 970, 1112
FaqI GGGAC 3 cut(s) 142, 538, 1033
FatI CATG 7 cut(s) 70, 270, 393, 684, 720, 966, 1108
FauI CCCGC 3 cut(s) 136, 336, 519
FblI GTMKAC 2 cut(s) 231, 426
Fnu4HI GCNGC 3 cut(s) 432, 753, 1053
FokI GGATG 2 cut(s) 335, 842
FriOI GRGCYC 2 cut(s) 150, 261
Fsp4HI GCNGC 3 cut(s) 432, 753, 1053
FspBI CTAG 1 cut(s) 566
GlaI GCGC 2 cut(s) 478, 480
GluI GCNGC 3 cut(s) 432, 753, 1053
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 6 cut(s) 105, 290, 521, 771, 811, 914
HgaI GACGC 3 cut(s) 197, 749, 972
HhaI GCGC 2 cut(s) 479, 481
Hin1I GRCGYC 2 cut(s) 189, 983
Hin1II CATG 7 cut(s) 74, 274, 397, 688, 724, 970, 1112
Hin6I GCGC 2 cut(s) 477, 479
HinP1I GCGC 2 cut(s) 477, 479
HincII GTYRAC 2 cut(s) 232, 442
HindII GTYRAC 2 cut(s) 232, 442
HindIII AAGCTT 1 cut(s) 85
HinfI GANTC 5 cut(s) 32, 56, 174, 692, 1021
HphI GGTGA 3 cut(s) 307, 314, 463
Hpy166II GTNNAC 7 cut(s) 232, 427, 442, 604, 610, 1092, 1159
Hpy188III TCNNGA 4 cut(s) 566, 638, 883, 1109
Hpy8I GTNNAC 7 cut(s) 232, 427, 442, 604, 610, 1092, 1159
Hpy99I CGWCG 6 cut(s) 191, 359, 470, 520, 788, 988
HpyAV CCTTC 2 cut(s) 141, 223
HpyCH4IV ACGT 7 cut(s) 165, 312, 606, 660, 786, 924, 938
HpyCH4V TGCA 3 cut(s) 19, 76, 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 670, 749
HpyF3I CTNAG 2 cut(s) 732, 881
HpySE526I ACGT 7 cut(s) 165, 312, 606, 660, 786, 924, 938
Hsp92I GRCGYC 2 cut(s) 189, 983
Hsp92II CATG 7 cut(s) 74, 274, 397, 688, 724, 970, 1112
HspAI GCGC 2 cut(s) 477, 479
Kzo9I GATC 5 cut(s) 7, 193, 300, 366, 1128
LmnI GCTCC 5 cut(s) 153, 264, 839, 1049, 1067
Lsp1109I GCAGC 1 cut(s) 1039
LweI GCATC 2 cut(s) 102, 718
MaeI CTAG 1 cut(s) 566
MaeII ACGT 7 cut(s) 165, 312, 606, 660, 786, 924, 938
MaeIII GTNAC 2 cut(s) 313, 469
MalI GATC 5 cut(s) 9, 195, 302, 368, 1130
MbiI CCGCTC 1 cut(s) 283
MboI GATC 5 cut(s) 7, 193, 300, 366, 1128
MboII GAAGA 6 cut(s) 548, 698, 910, 1073, 1092, 1123
MflI RGATCY 2 cut(s) 300, 366
MhlI GDGCHC 2 cut(s) 150, 261
MluCI AATT 6 cut(s) 24, 295, 561, 874, 888, 1135
MlyI GAGTC 3 cut(s) 26, 183, 1015
MmeI TCCRAC 4 cut(s) 243, 328, 966, 1027
Mph1103I ATGCAT 1 cut(s) 21
MseI TTAA 5 cut(s) 42, 83, 489, 957, 1116
MslI CAYNNNNRTG 1 cut(s) 413
MspCI CTTAAG 1 cut(s) 82
MspR9I CCNGG 4 cut(s) 523, 533, 859, 975
Mva1269I GAATGC 2 cut(s) 481, 859
MvnI CGCG 1 cut(s) 479
MwoI GCNNNNNNNGC 2 cut(s) 670, 749
NciI CCSGG 4 cut(s) 523, 533, 859, 975
NdeII GATC 5 cut(s) 7, 193, 300, 366, 1128
NlaIII CATG 7 cut(s) 74, 274, 397, 688, 724, 970, 1112
NlaIV GGNNCC 5 cut(s) 149, 302, 379, 700, 841
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 2 cut(s) 313, 469
NsiI ATGCAT 1 cut(s) 21
NspI RCATGY 1 cut(s) 724
PagI TCATGA 1 cut(s) 1108
PauI GCGCGC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 3 cut(s) 360, 378, 435
PctI GAATGC 2 cut(s) 481, 859
PfeI GAWTC 2 cut(s) 56, 692
PflFI GACNNNGTC 1 cut(s) 743
PfoI TCCNGGA 1 cut(s) 531
PkrI GCNGC 3 cut(s) 433, 754, 1054
PleI GAGTC 3 cut(s) 26, 182, 1015
PpsI GAGTC 3 cut(s) 26, 182, 1015
Ppu21I YACGTR 2 cut(s) 661, 939
PshAI GACNNNNGTC 1 cut(s) 387
PshBI ATTAAT 1 cut(s) 1116
Psp124BI GAGCTC 1 cut(s) 261
PspN4I GGNNCC 5 cut(s) 149, 302, 379, 700, 841
PspPI GGNCC 4 cut(s) 289, 769, 977, 1058
PsuI RGATCY 2 cut(s) 300, 366
PsyI GACNNNGTC 1 cut(s) 743
PteI GCGCGC 1 cut(s) 477
RsaI GTAC 4 cut(s) 401, 611, 659, 1160
RsaNI GTAC 4 cut(s) 400, 610, 658, 1159
RseI CAYNNNNRTG 1 cut(s) 413
Rsr2I CGGWCCG 1 cut(s) 977
RsrII CGGWCCG 1 cut(s) 977
SacI GAGCTC 1 cut(s) 261
SalI GTCGAC 1 cut(s) 230
SaqAI TTAA 5 cut(s) 42, 83, 489, 957, 1116
SatI GCNGC 3 cut(s) 432, 753, 1053
Sau3AI GATC 5 cut(s) 7, 193, 300, 366, 1128
Sau96I GGNCC 4 cut(s) 289, 769, 977, 1058
SchI GAGTC 3 cut(s) 26, 183, 1015
ScrFI CCNGG 4 cut(s) 523, 533, 859, 975
SduI GDGCHC 2 cut(s) 150, 261
SfaNI GCATC 2 cut(s) 102, 718
SinI GGWCC 2 cut(s) 977, 1058
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 2 cut(s) 82, 284
SmoI CTYRAG 2 cut(s) 82, 284
SnaBI TACGTA 1 cut(s) 939
Sse9I AATT 6 cut(s) 24, 295, 561, 874, 888, 1135
SsiI CCGC 7 cut(s) 143, 281, 329, 375, 432, 512, 753
SspMI CTAG 1 cut(s) 566
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 4 cut(s) 521, 531, 857, 973
StyI CCWWGG 2 cut(s) 651, 816
TaiI ACGT 7 cut(s) 168, 315, 609, 663, 789, 927, 941
TaqI TCGA 4 cut(s) 231, 726, 783, 1127
TaqII GACCGA 1 cut(s) 290
TasI AATT 6 cut(s) 24, 295, 561, 874, 888, 1135
TatI WGTACW 1 cut(s) 1158
TauI GCSGC 2 cut(s) 434, 755
TfiI GAWTC 2 cut(s) 56, 692
Tru1I TTAA 5 cut(s) 42, 83, 489, 957, 1116
Tru9I TTAA 5 cut(s) 42, 83, 489, 957, 1116
TscAI CASTG 2 cut(s) 83, 781
TseFI GTSAC 2 cut(s) 313, 469
TseI GCWGC 1 cut(s) 1052
Tsp45I GTSAC 2 cut(s) 313, 469
TspDTI ATGAA 6 cut(s) 226, 657, 701, 773, 846, 983
TspGWI ACGGA 2 cut(s) 412, 1080
TspRI CASTG 2 cut(s) 83, 781
Tth111I GACNNNGTC 1 cut(s) 743
Vha464I CTTAAG 1 cut(s) 82
VpaK11BI GGWCC 2 cut(s) 977, 1058
VspI ATTAAT 1 cut(s) 1116
XapI RAATTY 2 cut(s) 24, 874
XbaI TCTAGA 1 cut(s) 565
XceI RCATGY 1 cut(s) 724
XmiI GTMKAC 2 cut(s) 231, 426
XspI CTAG 1 cut(s) 566
Zsp2I ATGCAT 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.