AT2G30040
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015235
Physical Location & Seq
Forward (+)
12821569 .. 12823169
1601 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G30040.1

Sequence Viewer

Length: 1392 bp
ATGGAGAAACAGAACATTATCTCAAACACTTCTTCTTCTTCGTCTTCTTGGATTCGTGGTTCTTGTGTTGGAAGAGGTTGTTTTGGTACAGTAAGCAAAGCTTTGAGTAAAATCGACGGTGGACTTTTCGCCGTGAAGTCGATAGATCTCGCCACGTGTCTTCCTTCTCAAGCAGAGTCGTTAGAGAACGAAATCGTTATTCTACGGTCGATGAAGTCTCATCCAAACATAGTGAGGTTTCTCGGTGATGATGTGTCTAAAGAAGGAACGGCGTCGTTTCGAAATCTTCATTTAGAGTATTCACCGGAAGGTGACGTTGCTAACGGTGGAATCGTTAACGAAACGCTTCTCAGGCGTTACGTGTGGTGTCTTGTCTCTGCTTTGAGTCACGTTCATTCTAACGGAATTGTTCACTGCGACGTTAAATCTAAGAACGTTCTTGTTTTTAACGGTGGTAGCTCCGTTAAGCTAGCGGATTTTGGATCGGCGGTTGAGTTTGAGAAATCTACGATTCATGTTTCGCCACGTGGAAGTCCGCTTTGGATGGCTCCGGAGGTGGTTAGAAGAGAGTACCAAGGACCGGAGAGTGACGTGTGGTCTTTAGGGTGTACCGTCATCGAGATGCTCACCGGAAAACCAGCTTGGGAAGATCACGGTTTCGACTCGCTGAGTCGGATCGGGTTCTCTAACGATTTGCCTTTTATTCCGGTGGGGTTATCGGAACTCGGGAGAGATTTCTTAGAGAAATGCTTGAAACGTGATCGGAGTCAGCGTTGGAGCTGTGATCAGCTCTTGCAGCATCCGTTTCTGTGTCAAGATCATCATGACTCGTTCTTCACTGAGTCGTCTCCGCGTTGTGTTCTTGACTGGGTCAACTCGGAGTTTGACGAAGAAGAAGAGAGCGACGAATGGAGACCAGAATCCATGGTTTCGGCGATGGCAAGGATAAGTAAATTAGCTATAACCGGAGGAGCAAATTGGGAATCTAATGGTTGGACTGAGGTTAGAGACACTTCCGAAGAGTCAGAGGCAAAAAAGGAAGTTCTTGTTTCTCCAAGGGTAGAATTGGAATCTTACATATCATTGGAGTCGTCAAGCGATGATTCCGTAAGACAACCGAGGAATGAAGAGTCGGCGACGGAGTTGGCGTCGGCAGTGACGTGTGAAGCAATATTGTTGGTGATGATATTGGTGGTAGAGAATATTCAAATATATGCCACGTTTTACACCAGTAGTATTATTATGCATATTCTATATTGTTGTAGTTGTTGTTGTTATTATCATTATCAAAACAATAATAAGAAGAATAATTTTTCGAAATCTACATCTTTCATTCTCAGCCTTAACTTTTTGTTTGGTATTGCATGTGATTCGGATCGGTCTATCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000186 GO:0000746 GO:0000747 GO:0000749 GO:0000750 GO:0000935 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007186 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010514 GO:0010562 GO:0010564 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019236 GO:0019538 GO:0019953 GO:0022414 GO:0022603 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030428 GO:0031098 GO:0031136 GO:0031137 GO:0031139 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032005 GO:0032147 GO:0032153 GO:0032155 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0034306 GO:0034307 GO:0035556 GO:0036211 GO:0042173 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043067 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0043902 GO:0043937 GO:0043940 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0044764 GO:0045595 GO:0045859 GO:0045860 GO:0045937 GO:0046999 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051286 GO:0051338 GO:0051347 GO:0051445 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071444 GO:0071507 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902533 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

463

Amino Acids

51.67

Weight (kDa)

5.04

Isoelectric Point (pI)

60.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 21 - 270 5.1e-54 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 22 - 266 1.6e-24 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07150 AT1G07150 AT2G30040
fragaria_vesca FvH4_3g39010
malus_domestica MD03G1065400.v1.1 MD11G1070200.v1.1
prunus_persica Prupe.6G054400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0070321
rosa_laevigata RLG00000036124
rosa_multiflora Rmu_co8495105.1_g000001 Rmu_sc0009590.1_g000003
rosa_rugosa Rorug05G0404900
rosa_samantha Rh5AG460500 Rh5BG478800 Rh5CG503000
rosa_wichuraiana Rw5G042920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 853
AccIII TCCGGA 1 cut(s) 550
AciI CCGC 4 cut(s) 473, 488, 536, 851
AclI AACGTT 1 cut(s) 435
AclWI GGATC 3 cut(s) 490, 683, 1383
AcvI CACGTG 2 cut(s) 156, 527
AcyI GRCGYC 2 cut(s) 272, 1148
AfaI GTAC 3 cut(s) 88, 572, 610
AfiI CCNNNNNNNGG 1 cut(s) 580
AflIII ACRYGT 4 cut(s) 155, 360, 591, 1160
AgsI TTSAA 2 cut(s) 754, 1208
AhdI GACNNNNNGTC 1 cut(s) 595
AjiI CACGTC 2 cut(s) 592, 1161
AjuI GAANNNNNNNTTGG 4 cut(s) 523, 555, 625, 657
AloI GAACNNNNNNTCC 2 cut(s) 43, 75
AluBI AGCT 7 cut(s) 101, 459, 469, 641, 780, 790, 959
AluI AGCT 7 cut(s) 101, 459, 469, 641, 780, 790, 959
Alw26I GTCTC 5 cut(s) 222, 379, 852, 907, 1002
AlwI GGATC 3 cut(s) 490, 683, 1383
Ama87I CYCGRG 1 cut(s) 725
Aor13HI TCCGGA 1 cut(s) 550
ApeKI GCWGC 1 cut(s) 796
Asp700I GAANNNNTTC 1 cut(s) 345
AspS9I GGNCC 1 cut(s) 578
AsuHPI GGTGA 5 cut(s) 257, 294, 323, 619, 1192
AsuII TTCGAA 2 cut(s) 280, 1316
AsuNHI GCTAGC 1 cut(s) 469
AvaI CYCGRG 1 cut(s) 725
AvaII GGWCC 1 cut(s) 578
BbrPI CACGTG 2 cut(s) 156, 527
BbsI GAAGAC 2 cut(s) 36, 152
BbvI GCAGC 1 cut(s) 808
BccI CCATC 2 cut(s) 538, 931
BceAI ACGGC 2 cut(s) 116, 285
BcgI CGANNNNNNTGC 2 cut(s) 1353, 1387
BclI TGATCA 1 cut(s) 784
BcoDI GTCTC 5 cut(s) 222, 379, 852, 907, 1002
BfaI CTAG 1 cut(s) 470
BglII AGATCT 1 cut(s) 145
BisI GCNGC 1 cut(s) 797
BlsI GCNGC 1 cut(s) 798
Bme18I GGWCC 1 cut(s) 578
BmeRI GACNNNNNGTC 1 cut(s) 595
BmeT110I CYCGRG 1 cut(s) 725
BmgBI CACGTC 2 cut(s) 592, 1161
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 1 cut(s) 549
BmrI ACTGGG 1 cut(s) 877
BmsI GCATC 2 cut(s) 612, 808
BmtI GCTAGC 1 cut(s) 473
BmuI ACTGGG 1 cut(s) 877
BpiI GAAGAC 2 cut(s) 36, 152
Bpu14I TTCGAA 2 cut(s) 280, 1316
BpuEI CTTGAG 1 cut(s) 153
BsaAI YACGTR 3 cut(s) 156, 361, 527
BsaBI GATNNNNATC 1 cut(s) 1374
BsaHI GRCGYC 2 cut(s) 272, 1148
BsaI GGTCTC 1 cut(s) 907
BsaJI CCNNGG 4 cut(s) 574, 924, 1055, 1118
BsaWI WCCGGW 6 cut(s) 304, 550, 580, 629, 706, 965
BsaXI ACNNNNNCTCC 4 cut(s) 757, 787, 1133, 1163
Bsc4I CCNNNNNNNGG 1 cut(s) 580
Bse1I ACTGG 2 cut(s) 872, 1230
Bse8I GATNNNNATC 1 cut(s) 1374
BseAI TCCGGA 1 cut(s) 550
BseDI CCNNGG 4 cut(s) 574, 924, 1055, 1118
BseGI GGATG 3 cut(s) 220, 549, 799
BseJI GATNNNNATC 1 cut(s) 1374
BseLI CCNNNNNNNGG 1 cut(s) 580
BseMII CTCAG 5 cut(s) 364, 659, 831, 990, 1351
BseNI ACTGG 2 cut(s) 872, 1230
BseRI GAGGAG 1 cut(s) 984
BseXI GCAGC 1 cut(s) 808
Bsh1236I CGCG 1 cut(s) 853
Bsh1285I CGRYCG 1 cut(s) 209
BsiEI CGRYCG 1 cut(s) 209
BsiHKCI CYCGRG 1 cut(s) 725
BsiSI CCGG 6 cut(s) 305, 551, 581, 630, 707, 966
BslI CCNNNNNNNGG 1 cut(s) 580
BsmAI GTCTC 5 cut(s) 222, 379, 852, 907, 1002
BsmBI CGTCTC 1 cut(s) 852
Bso31I GGTCTC 1 cut(s) 907
BsoBI CYCGRG 1 cut(s) 725
Bsp119I TTCGAA 2 cut(s) 280, 1316
Bsp13I TCCGGA 1 cut(s) 550
Bsp143I GATC 8 cut(s) 145, 482, 649, 675, 760, 784, 817, 1375
Bsp19I CCATGG 1 cut(s) 924
BspACI CCGC 4 cut(s) 473, 488, 536, 851
BspCNI CTCAG 5 cut(s) 363, 660, 832, 991, 1350
BspEI TCCGGA 1 cut(s) 550
BspFNI CGCG 1 cut(s) 853
BspHI TCATGA 1 cut(s) 823
BspLI GGNNCC 1 cut(s) 549
BspOI GCTAGC 1 cut(s) 473
BspPI GGATC 3 cut(s) 490, 683, 1383
BspT104I TTCGAA 2 cut(s) 280, 1316
BspTNI GGTCTC 1 cut(s) 907
BsrI ACTGG 2 cut(s) 872, 1230
BssECI CCNNGG 4 cut(s) 574, 924, 1055, 1118
BssMI GATC 8 cut(s) 145, 482, 649, 675, 760, 784, 817, 1375
BssNI GRCGYC 2 cut(s) 272, 1148
BssT1I CCWWGG 3 cut(s) 574, 924, 1055
Bst4CI ACNGT 7 cut(s) 91, 119, 207, 326, 452, 613, 656
Bst6I CTCTTC 5 cut(s) 67, 559, 891, 1014, 1122
BstACI GRCGYC 2 cut(s) 272, 1148
BstBAI YACGTR 3 cut(s) 156, 361, 527
BstBI TTCGAA 2 cut(s) 280, 1316
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 7 cut(s) 350, 429, 668, 739, 840, 999, 1337
BstDSI CCRYGG 1 cut(s) 924
BstF5I GGATG 3 cut(s) 220, 549, 799
BstFNI CGCG 1 cut(s) 853
BstKTI GATC 8 cut(s) 148, 485, 652, 678, 763, 787, 820, 1378
BstMAI GTCTC 5 cut(s) 222, 379, 852, 907, 1002
BstMBI GATC 8 cut(s) 145, 482, 649, 675, 760, 784, 817, 1375
BstMCI CGRYCG 1 cut(s) 209
BstMWI GCNNNNNNNGC 2 cut(s) 352, 796
BstNSI RCATGY 1 cut(s) 1368
BstUI CGCG 1 cut(s) 853
BstV1I GCAGC 1 cut(s) 808
BstV2I GAAGAC 2 cut(s) 36, 152
BstX2I RGATCY 1 cut(s) 145
BstYI RGATCY 1 cut(s) 145
BtgI CCRYGG 1 cut(s) 924
BtgZI GCGATG 2 cut(s) 950, 1113
BtrI CACGTC 2 cut(s) 592, 1161
BtsCI GGATG 3 cut(s) 220, 549, 799
BtsI GCAGTG 2 cut(s) 412, 1161
BtsIMutI CAGTG 3 cut(s) 412, 837, 1161
Cac8I GCNNGC 1 cut(s) 471
CciI TCATGA 1 cut(s) 823
Cfr13I GGNCC 1 cut(s) 578
CseI GACGC 2 cut(s) 261, 1137
Csp6I GTAC 3 cut(s) 87, 571, 609
CviAII CATG 4 cut(s) 515, 824, 925, 1365
CviJI RGCY 9 cut(s) 101, 459, 469, 548, 641, 780, 790, 959, 1341
CviKI_1 RGCY 9 cut(s) 101, 459, 469, 548, 641, 780, 790, 959, 1341
CviQI GTAC 3 cut(s) 87, 571, 609
DdeI CTNAG 7 cut(s) 350, 429, 668, 739, 840, 999, 1337
DpnI GATC 8 cut(s) 147, 484, 651, 677, 762, 786, 819, 1377
DpnII GATC 8 cut(s) 145, 482, 649, 675, 760, 784, 817, 1375
DriI GACNNNNNGTC 1 cut(s) 595
Eam1104I CTCTTC 5 cut(s) 67, 559, 891, 1014, 1122
Eam1105I GACNNNNNGTC 1 cut(s) 595
EarI CTCTTC 5 cut(s) 67, 559, 891, 1014, 1122
Eco130I CCWWGG 3 cut(s) 574, 924, 1055
Eco31I GGTCTC 1 cut(s) 907
Eco47I GGWCC 1 cut(s) 578
Eco72I CACGTG 2 cut(s) 156, 527
Eco88I CYCGRG 1 cut(s) 725
EcoT14I CCWWGG 3 cut(s) 574, 924, 1055
EcoT22I ATGCAT 1 cut(s) 1248
ErhI CCWWGG 3 cut(s) 574, 924, 1055
Esp3I CGTCTC 1 cut(s) 852
FaeI CATG 4 cut(s) 518, 827, 928, 1368
FalI AAGNNNNNCTT 2 cut(s) 85, 117
FatI CATG 4 cut(s) 514, 823, 924, 1364
FbaI TGATCA 1 cut(s) 784
Fnu4HI GCNGC 1 cut(s) 797
FokI GGATG 3 cut(s) 207, 556, 786
Fsp4HI GCNGC 1 cut(s) 797
FspBI CTAG 1 cut(s) 470
GluI GCNGC 1 cut(s) 797
HapII CCGG 6 cut(s) 305, 551, 581, 630, 707, 966
HgaI GACGC 2 cut(s) 261, 1137
Hin1I GRCGYC 2 cut(s) 272, 1148
Hin1II CATG 4 cut(s) 518, 827, 928, 1368
HincII GTYRAC 2 cut(s) 337, 874
HindII GTYRAC 2 cut(s) 337, 874
HindIII AAGCTT 1 cut(s) 99
HpaI GTTAAC 1 cut(s) 337
HpaII CCGG 6 cut(s) 305, 551, 581, 630, 707, 966
HphI GGTGA 5 cut(s) 257, 294, 323, 619, 1192
Hpy166II GTNNAC 5 cut(s) 122, 337, 412, 609, 874
Hpy188I TCNGA 7 cut(s) 675, 721, 765, 880, 1018, 1027, 1375
Hpy188III TCNNGA 6 cut(s) 551, 619, 727, 815, 824, 863
Hpy8I GTNNAC 5 cut(s) 122, 337, 412, 609, 874
Hpy99I CGWCG 6 cut(s) 119, 277, 422, 908, 1141, 1153
HpyAV CCTTC 3 cut(s) 174, 257, 302
HpyCH4III ACNGT 7 cut(s) 91, 119, 207, 326, 452, 613, 656
HpyCH4V TGCA 3 cut(s) 796, 1246, 1364
HpyF10VI GCNNNNNNNGC 2 cut(s) 352, 796
HpyF3I CTNAG 7 cut(s) 350, 429, 668, 739, 840, 999, 1337
Hsp92I GRCGYC 2 cut(s) 272, 1148
Hsp92II CATG 4 cut(s) 518, 827, 928, 1368
Kpn2I TCCGGA 1 cut(s) 550
Ksp22I TGATCA 1 cut(s) 784
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 8 cut(s) 145, 482, 649, 675, 760, 784, 817, 1375
LmnI GCTCC 4 cut(s) 464, 553, 777, 971
Lsp1109I GCAGC 1 cut(s) 808
LweI GCATC 2 cut(s) 612, 808
MaeI CTAG 1 cut(s) 470
MaeIII GTNAC 5 cut(s) 311, 356, 386, 587, 1156
MalI GATC 8 cut(s) 147, 484, 651, 677, 762, 786, 819, 1377
MboI GATC 8 cut(s) 145, 482, 649, 675, 760, 784, 817, 1375
MflI RGATCY 1 cut(s) 145
MluCI AATT 5 cut(s) 405, 953, 976, 1064, 1309
MmeI TCCRAC 4 cut(s) 49, 653, 755, 974
MnlI CCTC 7 cut(s) 68, 228, 547, 962, 994, 1021, 1113
Mph1103I ATGCAT 1 cut(s) 1248
MroI TCCGGA 1 cut(s) 550
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 6 cut(s) 336, 423, 447, 465, 1344, 1390
MslI CAYNNNNRTG 1 cut(s) 620
MspI CCGG 6 cut(s) 305, 551, 581, 630, 707, 966
MvnI CGCG 1 cut(s) 853
MwoI GCNNNNNNNGC 2 cut(s) 352, 796
NcoI CCATGG 1 cut(s) 924
NdeII GATC 8 cut(s) 145, 482, 649, 675, 760, 784, 817, 1375
NheI GCTAGC 1 cut(s) 469
NlaIII CATG 4 cut(s) 518, 827, 928, 1368
NlaIV GGNNCC 1 cut(s) 549
NmuCI GTSAC 4 cut(s) 311, 386, 587, 1156
NsiI ATGCAT 1 cut(s) 1248
NspI RCATGY 1 cut(s) 1368
NspV TTCGAA 2 cut(s) 280, 1316
PagI TCATGA 1 cut(s) 823
PcsI WCGNNNNNNNCGW 4 cut(s) 330, 769, 1145, 1157
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 8 cut(s) 52, 330, 511, 920, 983, 1070, 1103, 1370
PflFI GACNNNGTC 1 cut(s) 869
PkrI GCNGC 1 cut(s) 798
PmaCI CACGTG 2 cut(s) 156, 527
PmlI CACGTG 2 cut(s) 156, 527
Ppu21I YACGTR 3 cut(s) 156, 361, 527
Psp1406I AACGTT 1 cut(s) 435
PspCI CACGTG 2 cut(s) 156, 527
PspN4I GGNNCC 1 cut(s) 549
PspPI GGNCC 1 cut(s) 578
PsuI RGATCY 1 cut(s) 145
PsyI GACNNNGTC 1 cut(s) 869
RsaI GTAC 3 cut(s) 88, 572, 610
RsaNI GTAC 3 cut(s) 87, 571, 609
RseI CAYNNNNRTG 1 cut(s) 620
SaqAI TTAA 6 cut(s) 336, 423, 447, 465, 1344, 1390
SatI GCNGC 1 cut(s) 797
Sau3AI GATC 8 cut(s) 145, 482, 649, 675, 760, 784, 817, 1375
Sau96I GGNCC 1 cut(s) 578
SfaNI GCATC 2 cut(s) 612, 808
SfuI TTCGAA 2 cut(s) 280, 1316
SinI GGWCC 1 cut(s) 578
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
Sse9I AATT 5 cut(s) 405, 953, 976, 1064, 1309
SsiI CCGC 4 cut(s) 473, 488, 536, 851
SspI AATATT 2 cut(s) 1173, 1204
SspMI CTAG 1 cut(s) 470
StyI CCWWGG 3 cut(s) 574, 924, 1055
TaaI ACNGT 7 cut(s) 91, 119, 207, 326, 452, 613, 656
TaqI TCGA 7 cut(s) 114, 140, 209, 280, 618, 660, 1316
TaqII GACCGA 1 cut(s) 1368
TasI AATT 5 cut(s) 405, 953, 976, 1064, 1309
TfiI GAWTC 8 cut(s) 52, 330, 511, 920, 983, 1070, 1103, 1370
Tru1I TTAA 6 cut(s) 336, 423, 447, 465, 1344, 1390
Tru9I TTAA 6 cut(s) 336, 423, 447, 465, 1344, 1390
TscAI CASTG 3 cut(s) 419, 844, 1161
TseFI GTSAC 4 cut(s) 311, 386, 587, 1156
TseI GCWGC 1 cut(s) 796
Tsp45I GTSAC 4 cut(s) 311, 386, 587, 1156
TspDTI ATGAA 6 cut(s) 227, 278, 383, 503, 1140, 1321
TspGWI ACGGA 5 cut(s) 417, 451, 792, 1096, 1154
TspRI CASTG 3 cut(s) 419, 844, 1161
Tth111I GACNNNGTC 1 cut(s) 869
VpaK11BI GGWCC 1 cut(s) 578
XceI RCATGY 1 cut(s) 1368
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 1 cut(s) 470
Zsp2I ATGCAT 1 cut(s) 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.