MD03G1065400.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015235
Physical Location & Seq
Reverse (-)
5211194 .. 5212624
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1065400.v1.1.491

Sequence Viewer

Length: 1431 bp
ATGGGAAAAATGAACTCAAACACTCAACATTCTCACGACACGGTTTCTTGGGTACGAGGAAAATGCATAGGAAGAGGGTCGTTCGGCACCGTCAGCATCGGGGTCAGCAAATCGGACGGTCGTGTCTTCGCCGTCAAGTCGGTCGATCAAGCCGCGTGCCTTCCCGGCCAGCTGGAGGCGCTGGAGAACGAAATTCGAATTCTCCGGTCGCTCTCCTCGCCGCACGTCGTGAGTTACCTCCGCGACGACGTTTCGTGCGAATCTGCGACGTCGTTTCGTAATCTCCACTTGGAGTACTTGCCAGGTGGCACCGCCGTGGACGCCGACGTGGACGAAACGACGCTGCGGTCGCGCGTTTGGTGCGTCGTTTCGGCGCTGAAGCACGTTCACTCCAAGGGCATTGTTCACTGCGACGTCAAGGGGAGGAATGTCCTGGTGGGCCCCGCGATTAATCAAGCCAAGCTCGCCGATTTCGGCTCCGCGATTGTCTTGTCGGATGACATGTGGACGAATAGAATCACGCCGCGAGGAAGTCCGCTTTGGATGGCGCCGGAGGTCATTAACGGGGAATATCAGGGGCCGGAGTCCGACGTATGGTCGTTGGGCTGCACGATTATCGAGATGGTGACCGGAAAGCCCGCGTGGGAGGATCGCGGGATGGAGACGCTGGCTCGGATCGGGTTGTCGGGTGAATTGCCCCGGTTCCCGACCCAGTTGTCGGAAACGGGCCGCGATTTTCTCGGCAAGTGTCTGAGTAGGGACCCGAAGGAGCGGTGGAGCTGCGATCAGCTGCTGCAGCACCCATTTTTGGCTTCCGCGTCGCTTAATGCAGTCGCCTATTCGTCGCCACGGTCCGTGCTCGACTGGGTTAACTCCGAGTTCGGTGATCAAACCGACGACGTCGATGGCGAGGACGAGGAGGAGTGCGAGGTTTCTGCCAGGAACCGGATTGGTAAATTGGCGACGGAGGCAGGGGCGAATTGGGAATCGGACGGATGGACGGTGGTGAGGGAATATTCAGGTGGCGCGAACTCTTGTGAGGAAGAAGGGACGAGTGAGGAATATTGTAATTGTGGAGGGGTAGAATTGGAATTAGACAACCTAAGTGGCGGTGGTTCAGTGGAGAGAGGTGTTAACTACAGTGGGTGGTGCGCGGTTGGCGCCAGCGGTTCGAAAAGGGAACATTTGGCGGTGGAAAGGTTAGGAGTTGGAAAAAGGTCACGTCAAAGCTTGTGTAATTTGTGCGGAAGCAAAGTATTATTATTATTTTTATATTTTATTGGGATATTCTTGAATAAATTACGAATTCTATTTATTTGTTATATTGTTTATGTTTCATTATCTTGGCATGGAGATATGCATGTGAATGTGATGATTGAACAAAGTCTAATCGTTGTTAAACTTAATACGTTAAACGAATCAAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000186 GO:0000746 GO:0000747 GO:0000749 GO:0000750 GO:0000935 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007186 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010514 GO:0010562 GO:0010564 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019236 GO:0019538 GO:0019953 GO:0022414 GO:0022603 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030428 GO:0031098 GO:0031136 GO:0031137 GO:0031139 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032005 GO:0032147 GO:0032153 GO:0032155 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0034306 GO:0034307 GO:0035556 GO:0036211 GO:0042173 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043067 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0043902 GO:0043937 GO:0043940 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0044764 GO:0045595 GO:0045859 GO:0045860 GO:0045937 GO:0046999 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051286 GO:0051338 GO:0051347 GO:0051445 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071444 GO:0071507 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902533 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

477

Amino Acids

52.09

Weight (kDa)

5.23

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 19 - 270 8.7e-54 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 21 - 266 3.1e-27 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07150 AT1G07150 AT2G30040
fragaria_vesca FvH4_3g39010
malus_domestica MD03G1065400.v1.1 MD11G1070200.v1.1
prunus_persica Prupe.6G054400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0070321
rosa_laevigata RLG00000036124
rosa_multiflora Rmu_co8495105.1_g000001 Rmu_sc0009590.1_g000003
rosa_rugosa Rorug05G0404900
rosa_samantha Rh5AG460500 Rh5BG478800 Rh5CG503000
rosa_wichuraiana Rw5G042920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 3 cut(s) 122, 346, 715
AatII GACGTC 3 cut(s) 272, 417, 903
AccB1I GGYRCC 4 cut(s) 86, 308, 547, 1160
AccBSI CCGCTC 1 cut(s) 772
AclWI GGATC 2 cut(s) 657, 683
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 3 cut(s) 192, 198, 1305
AcuI CTGAAG 1 cut(s) 398
AcyI GRCGYC 6 cut(s) 269, 321, 414, 548, 900, 1161
AdeI CACNNNGTG 1 cut(s) 229
AfaI GTAC 2 cut(s) 54, 296
AfiI CCNNNNNNNGG 5 cut(s) 175, 594, 718, 808, 945
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 2 cut(s) 1294, 1379
AhdI GACNNNNNGTC 1 cut(s) 595
AjiI CACGTC 3 cut(s) 226, 328, 1223
AjnI CCWGG 3 cut(s) 301, 432, 938
AjuI GAANNNNNNNTTGG 2 cut(s) 523, 555
AleI CACNNNNGTG 1 cut(s) 314
AluBI AGCT 5 cut(s) 172, 463, 780, 790, 1230
AluI AGCT 5 cut(s) 172, 463, 780, 790, 1230
Alw21I GWGCWC 1 cut(s) 861
Alw26I GTCTC 1 cut(s) 656
AlwI GGATC 2 cut(s) 657, 683
AlwNI CAGNNNCTG 1 cut(s) 793
AoxI GGCC 4 cut(s) 166, 439, 578, 727
ApaI GGGCCC 1 cut(s) 443
ApeKI GCWGC 6 cut(s) 343, 606, 780, 790, 793, 796
ApoI RAATTY 3 cut(s) 192, 198, 1305
AseI ATTAAT 1 cut(s) 450
AspLEI GCGC 7 cut(s) 181, 354, 376, 550, 1028, 1154, 1163
AspS9I GGNCC 6 cut(s) 439, 440, 578, 727, 760, 852
AsuC2I CCSGG 2 cut(s) 165, 700
AsuHPI GGTGA 4 cut(s) 637, 701, 896, 1018
AsuII TTCGAA 2 cut(s) 196, 1172
AvaII GGWCC 2 cut(s) 760, 852
BaeGI GKGCMC 1 cut(s) 443
BanI GGYRCC 4 cut(s) 86, 308, 547, 1160
BanII GRGCYC 1 cut(s) 443
BbsI GAAGAC 1 cut(s) 118
Bbv12I GWGCWC 1 cut(s) 861
BbvI GCAGC 6 cut(s) 330, 593, 767, 777, 780, 808
BccI CCATC 5 cut(s) 538, 616, 652, 899, 990
BceAI ACGGC 2 cut(s) 116, 299
BcgI CGANNNNNNTGC 6 cut(s) 45, 79, 598, 632, 917, 951
BciT130I CCWGG 3 cut(s) 303, 434, 940
BclI TGATCA 1 cut(s) 886
BcnI CCSGG 2 cut(s) 165, 700
BcoDI GTCTC 1 cut(s) 656
BfmI CTRYAG 2 cut(s) 794, 1138
BfoI RGCGCY 4 cut(s) 182, 377, 551, 1164
BglI GCCNNNNNGGC 1 cut(s) 165
BmcAI AGTACT 1 cut(s) 296
Bme1390I CCNGG 5 cut(s) 165, 303, 434, 700, 940
Bme18I GGWCC 2 cut(s) 760, 852
BmeRI GACNNNNNGTC 1 cut(s) 595
BmgBI CACGTC 3 cut(s) 226, 328, 1223
BmgT120I GGNCC 6 cut(s) 439, 440, 578, 727, 760, 852
BmrFI CCNGG 5 cut(s) 165, 303, 434, 700, 940
BmrI ACTGGG 2 cut(s) 706, 874
BmsI GCATC 1 cut(s) 105
BmuI ACTGGG 2 cut(s) 706, 874
BpiI GAAGAC 1 cut(s) 118
BpmI CTGGAG 2 cut(s) 194, 203
Bpu14I TTCGAA 2 cut(s) 196, 1172
BpuMI CCSGG 2 cut(s) 165, 700
BsaHI GRCGYC 6 cut(s) 269, 321, 414, 548, 900, 1161
BsaJI CCNNGG 4 cut(s) 315, 393, 698, 848
BsaWI WCCGGW 3 cut(s) 204, 629, 945
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 5 cut(s) 175, 594, 718, 808, 945
Bse1I ACTGG 2 cut(s) 712, 869
BseBI CCWGG 3 cut(s) 303, 434, 940
BseDI CCNNGG 4 cut(s) 315, 393, 698, 848
BseGI GGATG 4 cut(s) 502, 549, 663, 1001
BseLI CCNNNNNNNGG 5 cut(s) 175, 594, 718, 808, 945
BseMII CTCAG 1 cut(s) 743
BseNI ACTGG 2 cut(s) 712, 869
BseRI GAGGAG 3 cut(s) 205, 932, 935
BseSI GKGCMC 1 cut(s) 443
BseXI GCAGC 6 cut(s) 330, 593, 767, 777, 780, 808
BsgI GTGCAG 1 cut(s) 592
Bsh1285I CGRYCG 4 cut(s) 121, 144, 209, 350
BshFI GGCC 4 cut(s) 168, 441, 580, 729
BshNI GGYRCC 4 cut(s) 86, 308, 547, 1160
BsiEI CGRYCG 4 cut(s) 121, 144, 209, 350
BsiHKAI GWGCWC 1 cut(s) 861
BsiSI CCGG 7 cut(s) 165, 205, 551, 581, 630, 700, 946
BslFI GGGAC 2 cut(s) 773, 1063
BslI CCNNNNNNNGG 5 cut(s) 175, 594, 718, 808, 945
BsmAI GTCTC 1 cut(s) 656
BsmBI CGTCTC 1 cut(s) 656
BsmFI GGGAC 2 cut(s) 773, 1063
BsnI GGCC 4 cut(s) 168, 441, 580, 729
Bsp119I TTCGAA 2 cut(s) 196, 1172
Bsp120I GGGCCC 1 cut(s) 439
Bsp1286I GDGCHC 2 cut(s) 443, 861
Bsp143I GATC 5 cut(s) 145, 649, 675, 784, 886
BspANI GGCC 4 cut(s) 168, 441, 580, 729
BspCNI CTCAG 1 cut(s) 744
BspMAI CTGCAG 1 cut(s) 798
BspPI GGATC 2 cut(s) 657, 683
BspT104I TTCGAA 2 cut(s) 196, 1172
BspT107I GGYRCC 4 cut(s) 86, 308, 547, 1160
BsrBI CCGCTC 1 cut(s) 772
BsrI ACTGG 2 cut(s) 712, 869
BssECI CCNNGG 4 cut(s) 315, 393, 698, 848
BssMI GATC 5 cut(s) 145, 649, 675, 784, 886
BssNI GRCGYC 6 cut(s) 269, 321, 414, 548, 900, 1161
BssT1I CCWWGG 1 cut(s) 393
Bst2UI CCWGG 3 cut(s) 303, 434, 940
Bst4CI ACNGT 6 cut(s) 43, 91, 119, 852, 1003, 1142
Bst6I CTCTTC 1 cut(s) 67
BstACI GRCGYC 6 cut(s) 269, 321, 414, 548, 900, 1161
BstBI TTCGAA 2 cut(s) 196, 1172
BstC8I GCNNGC 6 cut(s) 157, 170, 465, 639, 669, 1165
BstDEI CTNAG 2 cut(s) 752, 1103
BstDSI CCRYGG 2 cut(s) 315, 848
BstEII GGTNACC 1 cut(s) 625
BstF5I GGATG 4 cut(s) 502, 549, 663, 1001
BstH2I RGCGCY 4 cut(s) 182, 377, 551, 1164
BstHHI GCGC 7 cut(s) 181, 354, 376, 550, 1028, 1154, 1163
BstKTI GATC 5 cut(s) 148, 652, 678, 787, 889
BstMAI GTCTC 1 cut(s) 656
BstMBI GATC 5 cut(s) 145, 649, 675, 784, 886
BstMCI CGRYCG 4 cut(s) 121, 144, 209, 350
BstNI CCWGG 3 cut(s) 303, 434, 940
BstNSI RCATGY 2 cut(s) 505, 1364
BstPI GGTNACC 1 cut(s) 625
BstSCI CCNGG 5 cut(s) 163, 301, 432, 698, 938
BstSFI CTRYAG 2 cut(s) 794, 1138
BstSLI GKGCMC 1 cut(s) 443
BstV1I GCAGC 6 cut(s) 330, 593, 767, 777, 780, 808
BstV2I GAAGAC 1 cut(s) 118
BsuRI GGCC 4 cut(s) 168, 441, 580, 729
BtgI CCRYGG 2 cut(s) 315, 848
BtrI CACGTC 3 cut(s) 226, 328, 1223
BtsCI GGATG 4 cut(s) 502, 549, 663, 1001
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 3 cut(s) 406, 1125, 1147
Cac8I GCNNGC 6 cut(s) 157, 170, 465, 639, 669, 1165
CaiI CAGNNNCTG 1 cut(s) 793
CfoI GCGC 7 cut(s) 181, 354, 376, 550, 1028, 1154, 1163
Cfr13I GGNCC 6 cut(s) 439, 440, 578, 727, 760, 852
CpoI CGGWCCG 1 cut(s) 852
CseI GACGC 5 cut(s) 329, 349, 352, 673, 807
Csp6I GTAC 2 cut(s) 53, 295
CspCI CAANNNNNGTGG 2 cut(s) 1087, 1122
CspI CGGWCCG 1 cut(s) 852
CviAII CATG 3 cut(s) 502, 1349, 1361
CviQI GTAC 2 cut(s) 53, 295
DdeI CTNAG 2 cut(s) 752, 1103
DinI GGCGCC 2 cut(s) 549, 1162
DpnI GATC 5 cut(s) 147, 651, 677, 786, 888
DpnII GATC 5 cut(s) 145, 649, 675, 784, 886
DraIII CACNNNGTG 1 cut(s) 229
DrdI GACNNNNNNGTC 3 cut(s) 122, 346, 715
DriI GACNNNNNGTC 1 cut(s) 595
DseDI GACNNNNNNGTC 3 cut(s) 122, 346, 715
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 67
Eam1105I GACNNNNNGTC 1 cut(s) 595
EarI CTCTTC 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 443
Eco47I GGWCC 2 cut(s) 760, 852
Eco57I CTGAAG 1 cut(s) 398
Eco91I GGTNACC 1 cut(s) 625
EcoO109I RGGNCCY 2 cut(s) 440, 760
EcoO65I GGTNACC 1 cut(s) 625
EcoRI GAATTC 2 cut(s) 198, 1305
EcoRII CCWGG 3 cut(s) 301, 432, 938
EcoT14I CCWWGG 1 cut(s) 393
EcoT22I ATGCAT 2 cut(s) 68, 1362
EcoT38I GRGCYC 1 cut(s) 443
EgeI GGCGCC 2 cut(s) 549, 1162
EheI GGCGCC 2 cut(s) 549, 1162
ErhI CCWWGG 1 cut(s) 393
Esp3I CGTCTC 1 cut(s) 656
FaeI CATG 3 cut(s) 505, 1352, 1364
FaiI YATR 9 cut(s) 68, 503, 595, 1273, 1323, 1332, 1350, 1358, 1362
FaqI GGGAC 2 cut(s) 773, 1063
FatI CATG 3 cut(s) 501, 1348, 1360
FauI CCCGC 3 cut(s) 451, 646, 647
FbaI TGATCA 1 cut(s) 886
FokI GGATG 4 cut(s) 509, 556, 670, 1008
FriOI GRGCYC 1 cut(s) 443
GlaI GCGC 7 cut(s) 180, 353, 375, 549, 1027, 1153, 1162
GsuI CTGGAG 2 cut(s) 194, 203
HaeII RGCGCY 4 cut(s) 182, 377, 551, 1164
HaeIII GGCC 4 cut(s) 168, 441, 580, 729
HapII CCGG 7 cut(s) 165, 205, 551, 581, 630, 700, 946
HgaI GACGC 5 cut(s) 329, 349, 352, 673, 807
HhaI GCGC 7 cut(s) 181, 354, 376, 550, 1028, 1154, 1163
Hin1I GRCGYC 6 cut(s) 269, 321, 414, 548, 900, 1161
Hin1II CATG 3 cut(s) 505, 1352, 1364
Hin6I GCGC 7 cut(s) 179, 352, 374, 548, 1026, 1152, 1161
HinP1I GCGC 7 cut(s) 179, 352, 374, 548, 1026, 1152, 1161
HincII GTYRAC 2 cut(s) 871, 1135
HindII GTYRAC 2 cut(s) 871, 1135
HindIII AAGCTT 1 cut(s) 1228
HinfI GANTC 5 cut(s) 260, 516, 584, 986, 1418
HpaI GTTAAC 2 cut(s) 871, 1135
HpaII CCGG 7 cut(s) 165, 205, 551, 581, 630, 700, 946
HphI GGTGA 4 cut(s) 637, 701, 896, 1018
Hpy166II GTNNAC 7 cut(s) 319, 331, 388, 406, 507, 871, 1135
Hpy188I TCNGA 8 cut(s) 115, 496, 589, 675, 721, 753, 877, 991
Hpy188III TCNNGA 5 cut(s) 35, 229, 619, 706, 1291
Hpy8I GTNNAC 7 cut(s) 319, 331, 388, 406, 507, 871, 1135
HpyAV CCTTC 3 cut(s) 170, 760, 1040
HpyCH4III ACNGT 6 cut(s) 43, 91, 119, 852, 1003, 1142
HpyCH4V TGCA 5 cut(s) 66, 609, 796, 830, 1360
HpyF3I CTNAG 2 cut(s) 752, 1103
Hsp92I GRCGYC 6 cut(s) 269, 321, 414, 548, 900, 1161
Hsp92II CATG 3 cut(s) 505, 1352, 1364
HspAI GCGC 7 cut(s) 179, 352, 374, 548, 1026, 1152, 1161
KasI GGCGCC 2 cut(s) 547, 1160
KflI GGGWCCC 1 cut(s) 760
Ksp22I TGATCA 1 cut(s) 886
KspAI GTTAAC 2 cut(s) 871, 1135
Kzo9I GATC 5 cut(s) 145, 649, 675, 784, 886
LmnI GCTCC 3 cut(s) 482, 769, 777
Lsp1109I GCAGC 6 cut(s) 330, 593, 767, 777, 780, 808
LweI GCATC 1 cut(s) 105
MaeIII GTNAC 3 cut(s) 233, 625, 1218
MalI GATC 5 cut(s) 147, 651, 677, 786, 888
MbiI CCGCTC 1 cut(s) 772
MboI GATC 5 cut(s) 145, 649, 675, 784, 886
MboII GAAGA 3 cut(s) 84, 118, 1055
MhlI GDGCHC 2 cut(s) 443, 861
Mly113I GGCGCC 2 cut(s) 548, 1161
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 4 cut(s) 474, 612, 699, 1189
Mph1103I ATGCAT 2 cut(s) 68, 1362
MseI TTAA 8 cut(s) 450, 561, 825, 870, 1134, 1398, 1404, 1412
MslI CAYNNNNRTG 2 cut(s) 314, 1365
MspA1I CMGCKG 3 cut(s) 172, 790, 1167
MspI CCGG 7 cut(s) 165, 205, 551, 581, 630, 700, 946
MspR9I CCNGG 5 cut(s) 165, 303, 434, 700, 940
MvaI CCWGG 3 cut(s) 303, 434, 940
NarI GGCGCC 2 cut(s) 548, 1161
NciI CCSGG 2 cut(s) 165, 700
NdeII GATC 5 cut(s) 145, 649, 675, 784, 886
NlaIII CATG 3 cut(s) 505, 1352, 1364
NmeAIII GCCGAG 1 cut(s) 720
NmuCI GTSAC 2 cut(s) 625, 1218
NsiI ATGCAT 2 cut(s) 68, 1362
NspI RCATGY 2 cut(s) 505, 1364
NspV TTCGAA 2 cut(s) 196, 1172
OliI CACNNNNGTG 1 cut(s) 314
PciI ACATGT 1 cut(s) 501
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 906
PfeI GAWTC 4 cut(s) 260, 516, 986, 1418
PflFI GACNNNGTC 1 cut(s) 899
PleI GAGTC 1 cut(s) 592
PluTI GGCGCC 2 cut(s) 551, 1164
PpsI GAGTC 1 cut(s) 592
PpuMI RGGWCCY 1 cut(s) 760
PscI ACATGT 1 cut(s) 501
PshBI ATTAAT 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 760
Psp6I CCWGG 3 cut(s) 301, 432, 938
PspEI GGTNACC 1 cut(s) 625
PspGI CCWGG 3 cut(s) 301, 432, 938
PspOMI GGGCCC 1 cut(s) 439
PspPI GGNCC 6 cut(s) 439, 440, 578, 727, 760, 852
PspPPI RGGWCCY 1 cut(s) 760
PstI CTGCAG 1 cut(s) 798
PstNI CAGNNNCTG 1 cut(s) 793
PsyI GACNNNGTC 1 cut(s) 899
PvuII CAGCTG 2 cut(s) 172, 790
RsaI GTAC 2 cut(s) 54, 296
RsaNI GTAC 2 cut(s) 53, 295
RseI CAYNNNNRTG 2 cut(s) 314, 1365
Rsr2I CGGWCCG 1 cut(s) 852
RsrII CGGWCCG 1 cut(s) 852
SaqAI TTAA 8 cut(s) 450, 561, 825, 870, 1134, 1398, 1404, 1412
Sau3AI GATC 5 cut(s) 145, 649, 675, 784, 886
Sau96I GGNCC 6 cut(s) 439, 440, 578, 727, 760, 852
ScaI AGTACT 1 cut(s) 296
SchI GAGTC 1 cut(s) 593
ScrFI CCNGG 5 cut(s) 165, 303, 434, 700, 940
SduI GDGCHC 2 cut(s) 443, 861
SfaNI GCATC 1 cut(s) 105
SfcI CTRYAG 2 cut(s) 794, 1138
SfoI GGCGCC 2 cut(s) 549, 1162
SfuI TTCGAA 2 cut(s) 196, 1172
SinI GGWCC 2 cut(s) 760, 852
SmiMI CAYNNNNRTG 2 cut(s) 314, 1365
SspDI GGCGCC 2 cut(s) 547, 1160
SspI AATATT 2 cut(s) 1016, 1064
StyD4I CCNGG 5 cut(s) 163, 301, 432, 698, 938
StyI CCWWGG 1 cut(s) 393
TaaI ACNGT 6 cut(s) 43, 91, 119, 852, 1003, 1142
TaqI TCGA 6 cut(s) 144, 196, 618, 861, 903, 1172
TaqII GACCGA 1 cut(s) 130
TatI WGTACW 1 cut(s) 294
TauI GCSGC 4 cut(s) 155, 223, 526, 732
TfiI GAWTC 4 cut(s) 260, 516, 986, 1418
Tru1I TTAA 8 cut(s) 450, 561, 825, 870, 1134, 1398, 1404, 1412
Tru9I TTAA 8 cut(s) 450, 561, 825, 870, 1134, 1398, 1404, 1412
TscAI CASTG 3 cut(s) 413, 1125, 1147
TseFI GTSAC 2 cut(s) 625, 1218
TseI GCWGC 6 cut(s) 343, 606, 780, 790, 793, 796
Tsp45I GTSAC 2 cut(s) 625, 1218
TspDTI ATGAA 2 cut(s) 26, 1326
TspGWI ACGGA 3 cut(s) 844, 980, 1008
TspRI CASTG 3 cut(s) 413, 1125, 1147
Tth111I GACNNNGTC 1 cut(s) 899
VpaK11BI GGWCC 2 cut(s) 760, 852
VspI ATTAAT 1 cut(s) 450
XapI RAATTY 3 cut(s) 192, 198, 1305
XceI RCATGY 2 cut(s) 505, 1364
ZraI GACGTC 3 cut(s) 270, 415, 901
ZrmI AGTACT 1 cut(s) 296
Zsp2I ATGCAT 2 cut(s) 68, 1362
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.