MD11G1070200.v1.1
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015235
Physical Location & Seq
Reverse (-)
6003042 .. 6004451
1410 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1070200.v1.1.491

Sequence Viewer

Length: 1410 bp
ATGGGGAAAATGAACTCAAACTCTCAGCATTCTCACGACACGGTTTCTTGGGTACGAGGAAAATGCATAGGAAGGGGGTCGTTCGGCACCGTCAGCATCGGGGTCAGCAAACAGGACGGTCGTGTCTTCGCCGCCAAATCGGTCGATCAAGCCGCGTGTCTTCCCGGCCAGCTGGAGGCGCTGGAGAATGAAATTCGAATTGTCCGGTCGCTCTCCTCACCACACGTCGTGAGTTACCTCAGCGACGACGTGTCGTGCGAACCGGGGAGCGGTTCCGCGACGTCGTTTCGGAACCTCCACTTGGAGTACTTGCCAGGTGGCACCGCAGTGGATGCTGAAGTGGACGAAACGACGCTGCGGTCGCGCGTTTGGTGCGTCGTTGCGGCGCTGAGGGACGTTCACTCGAAGGGCATTGTTCACTGCGACGTCAAGGGTAAAAATGTCCTGGTGGGCCCCGCTATTAACCAAGCCAAGCTCGCCGATTTCGGTTCTGCGATTATCTTGTCGGATGACACGTGTACGGCCAGGATCATACCACGAGGAAGTCCGCTTTGGATGGCACCGGAGGTAATAAACGGGCAATATCAGGGACCGGAGTCCGACGTATGGTCGTTAGGCTGCACGGTTATCGAGATGGTGACCGGAAAGCCCGGGTGGGAGGATCGCGGTTTGGAGACGCTGGCTCGGATCGCGGTTTCGGACGGGTTGCCCCGGTTCCCGAGCCGGTTGTCGGAAACTGGTCGCGACTTTCTCGACAAGTGTCTGAGGAGGGACCCGAAGCAGCGGTGGAGCTGCGATCAGTTGCTGCAGCATCCATTTTTGGCTTCCCTGTCTCCAAATGCGGTCGCCGCCGACTCGTCACCTCGCTGCGTTCTCGACTGGGCTAACTCCGAGTTCGGCGATGAAAGCGACGTTAAGGATGACGAGGACGATGAGGAGTACGAGGTTTCTGCTAGGAATAGAATTGGTAAATTGGCGACGACGGGAGCGGCAAACTGGGAATCGGATGGATGGACGGTGGTGAGGAATTATTCAGGTGGCGGGAGGTCTTGCGAGGAAGAAGGGACAAGCGGGGAGAATTCGGATTGTGGAGGGGTAGAATTGGAAACTGAGAGCTCTAGTGGCGGTGGCTCAGTGGAGCGGGGTGTTAATAACTATGGGCGGTGCGCGGTTGGCGCCAGCGGTTCGAAAAGGGAATACTTGGCGGTGGAAAGGGTAGGAAATAGAGGAAGGGATTGTCAAACCTTGTCTATTTTGTGTAGAAGCAGATTATTATTATTATTATTGTTGTTGTTGTTTTTATATTCTTTGGGGATATTTAATAAATTACGAATTCTGTTTATTTGTTATATTGTTTATATTTCACAATCTTGGTGGAGCATGTTGTTAAAGTTTATTTTTGGGTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000186 GO:0000746 GO:0000747 GO:0000749 GO:0000750 GO:0000935 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007186 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010514 GO:0010562 GO:0010564 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019236 GO:0019538 GO:0019953 GO:0022414 GO:0022603 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030428 GO:0031098 GO:0031136 GO:0031137 GO:0031139 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032005 GO:0032147 GO:0032153 GO:0032155 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0034306 GO:0034307 GO:0035556 GO:0036211 GO:0042173 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043067 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0043902 GO:0043937 GO:0043940 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0044764 GO:0045595 GO:0045859 GO:0045860 GO:0045937 GO:0046999 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051286 GO:0051338 GO:0051347 GO:0051445 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071444 GO:0071507 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902533 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

470

Amino Acids

51.04

Weight (kDa)

5.31

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 19 - 274 2.9e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 21 - 270 1e-22 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07150 AT1G07150 AT2G30040
fragaria_vesca FvH4_3g39010
malus_domestica MD03G1065400.v1.1 MD11G1070200.v1.1
prunus_persica Prupe.6G054400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0070321
rosa_laevigata RLG00000036124
rosa_multiflora Rmu_co8495105.1_g000001 Rmu_sc0009590.1_g000003
rosa_rugosa Rorug05G0404900
rosa_samantha Rh5AG460500 Rh5BG478800 Rh5CG503000
rosa_wichuraiana Rw5G042920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 122, 358
AatII GACGTC 2 cut(s) 284, 429
AccB1I GGYRCC 4 cut(s) 86, 320, 559, 1175
AccBSI CCGCTC 3 cut(s) 270, 989, 1141
AccII CGCG 8 cut(s) 155, 278, 364, 366, 666, 692, 744, 1169
AclWI GGATC 3 cut(s) 536, 669, 695
AcoI YGGCCR 2 cut(s) 166, 522
AcsI RAATTY 3 cut(s) 192, 1078, 1332
AcuI CTGAAG 1 cut(s) 357
AcvI CACGTG 1 cut(s) 516
AcyI GRCGYC 3 cut(s) 281, 426, 1176
AdeI CACNNNGTG 1 cut(s) 229
AfaI GTAC 4 cut(s) 54, 308, 520, 941
AfiI CCNNNNNNNGG 5 cut(s) 175, 269, 301, 606, 730
AflIII ACRYGT 4 cut(s) 223, 249, 513, 515
AhdI GACNNNNNGTC 2 cut(s) 250, 607
AjiI CACGTC 2 cut(s) 226, 250
AjnI CCWGG 3 cut(s) 313, 444, 524
AjuI GAANNNNNNNTTGG 4 cut(s) 284, 316, 535, 567
AleI CACNNNNGTG 1 cut(s) 326
AluBI AGCT 4 cut(s) 172, 475, 792, 1116
AluI AGCT 4 cut(s) 172, 475, 792, 1116
Alw21I GWGCWC 1 cut(s) 1118
Alw26I GTCTC 2 cut(s) 668, 837
AlwI GGATC 3 cut(s) 536, 669, 695
AlwNI CAGNNNCTG 1 cut(s) 805
Ama87I CYCGRG 2 cut(s) 650, 718
AoxI GGCC 3 cut(s) 166, 451, 522
ApaI GGGCCC 1 cut(s) 455
ApeKI GCWGC 7 cut(s) 355, 618, 781, 792, 805, 808, 867
ApoI RAATTY 3 cut(s) 192, 1078, 1332
AspLEI GCGC 5 cut(s) 181, 366, 388, 1169, 1178
AspS9I GGNCC 4 cut(s) 451, 452, 590, 772
AsuC2I CCSGG 5 cut(s) 165, 264, 651, 652, 712
AsuHPI GGTGA 4 cut(s) 210, 649, 852, 1033
AsuII TTCGAA 2 cut(s) 196, 1187
AvaI CYCGRG 2 cut(s) 650, 718
AvaII GGWCC 2 cut(s) 590, 772
BaeGI GKGCMC 1 cut(s) 455
BanI GGYRCC 4 cut(s) 86, 320, 559, 1175
BanII GRGCYC 2 cut(s) 455, 1118
BauI CACGAG 1 cut(s) 537
BbrPI CACGTG 1 cut(s) 516
BbsI GAAGAC 2 cut(s) 118, 152
Bbv12I GWGCWC 1 cut(s) 1118
BbvCI CCTCAGC 2 cut(s) 239, 389
BbvI GCAGC 7 cut(s) 342, 605, 779, 792, 793, 820, 854
BccI CCATC 4 cut(s) 550, 628, 1001, 1005
BceAI ACGGC 1 cut(s) 537
BcgI CGANNNNNNTGC 6 cut(s) 45, 79, 610, 644, 932, 966
BciT130I CCWGG 3 cut(s) 315, 446, 526
BcnI CCSGG 5 cut(s) 165, 264, 651, 652, 712
BcoDI GTCTC 2 cut(s) 668, 837
BfaI CTAG 2 cut(s) 954, 1119
BfmI CTRYAG 1 cut(s) 806
BfoI RGCGCY 3 cut(s) 182, 389, 1179
BmcAI AGTACT 1 cut(s) 308
Bme1390I CCNGG 8 cut(s) 165, 264, 315, 446, 526, 651, 652, 712
Bme18I GGWCC 2 cut(s) 590, 772
BmeRI GACNNNNNGTC 2 cut(s) 250, 607
BmeT110I CYCGRG 2 cut(s) 650, 718
BmgBI CACGTC 2 cut(s) 226, 250
BmgT120I GGNCC 4 cut(s) 451, 452, 590, 772
BmrFI CCNGG 8 cut(s) 165, 264, 315, 446, 526, 651, 652, 712
BmrI ACTGGG 2 cut(s) 889, 1006
BmsI GCATC 3 cut(s) 105, 322, 820
BmuI ACTGGG 2 cut(s) 889, 1006
BoxI GACNNNNGTC 2 cut(s) 595, 759
BpiI GAAGAC 2 cut(s) 118, 152
BpmI CTGGAG 2 cut(s) 194, 203
Bpu10I CCTNAGC 2 cut(s) 239, 389
Bpu14I TTCGAA 2 cut(s) 196, 1187
BpuMI CCSGG 5 cut(s) 165, 264, 651, 652, 712
BsaAI YACGTR 1 cut(s) 516
BsaHI GRCGYC 3 cut(s) 281, 426, 1176
BsaJI CCNNGG 3 cut(s) 263, 650, 710
BsaWI WCCGGW 4 cut(s) 204, 562, 592, 641
Bsc4I CCNNNNNNNGG 5 cut(s) 175, 269, 301, 606, 730
Bse118I RCCGGY 1 cut(s) 723
Bse1I ACTGG 3 cut(s) 742, 884, 1001
BseBI CCWGG 3 cut(s) 315, 446, 526
BseDI CCNNGG 3 cut(s) 263, 650, 710
BseGI GGATG 7 cut(s) 337, 514, 561, 811, 925, 1012, 1016
BseLI CCNNNNNNNGG 5 cut(s) 175, 269, 301, 606, 730
BseMII CTCAG 6 cut(s) 38, 253, 380, 755, 1101, 1146
BseNI ACTGG 3 cut(s) 742, 884, 1001
BseRI GAGGAG 3 cut(s) 205, 781, 950
BseSI GKGCMC 1 cut(s) 455
BseXI GCAGC 7 cut(s) 342, 605, 779, 792, 793, 820, 854
BsgI GTGCAG 1 cut(s) 604
Bsh1236I CGCG 8 cut(s) 155, 278, 364, 366, 666, 692, 744, 1169
Bsh1285I CGRYCG 5 cut(s) 121, 144, 209, 362, 846
BshFI GGCC 3 cut(s) 168, 453, 524
BshNI GGYRCC 4 cut(s) 86, 320, 559, 1175
BsiEI CGRYCG 5 cut(s) 121, 144, 209, 362, 846
BsiHKAI GWGCWC 1 cut(s) 1118
BsiHKCI CYCGRG 2 cut(s) 650, 718
BsiSI CCGG 9 cut(s) 165, 205, 263, 563, 593, 642, 651, 712, 724
BslFI GGGAC 4 cut(s) 407, 603, 785, 1078
BslI CCNNNNNNNGG 5 cut(s) 175, 269, 301, 606, 730
BsmAI GTCTC 2 cut(s) 668, 837
BsmBI CGTCTC 1 cut(s) 668
BsmFI GGGAC 4 cut(s) 407, 603, 785, 1078
BsmI GAATGC 1 cut(s) 28
BsnI GGCC 3 cut(s) 168, 453, 524
BsoBI CYCGRG 2 cut(s) 650, 718
Bsp119I TTCGAA 2 cut(s) 196, 1187
Bsp120I GGGCCC 1 cut(s) 451
Bsp1286I GDGCHC 2 cut(s) 455, 1118
Bsp143I GATC 5 cut(s) 145, 528, 661, 687, 796
Bsp68I TCGCGA 1 cut(s) 744
BspANI GGCC 3 cut(s) 168, 453, 524
BspCNI CTCAG 6 cut(s) 37, 252, 381, 756, 1102, 1145
BspFNI CGCG 8 cut(s) 155, 278, 364, 366, 666, 692, 744, 1169
BspMAI CTGCAG 1 cut(s) 810
BspPI GGATC 3 cut(s) 536, 669, 695
BspT104I TTCGAA 2 cut(s) 196, 1187
BspT107I GGYRCC 4 cut(s) 86, 320, 559, 1175
BsrBI CCGCTC 3 cut(s) 270, 989, 1141
BsrFI RCCGGY 1 cut(s) 723
BsrI ACTGG 3 cut(s) 742, 884, 1001
BssAI RCCGGY 1 cut(s) 723
BssECI CCNNGG 3 cut(s) 263, 650, 710
BssMI GATC 5 cut(s) 145, 528, 661, 687, 796
BssNI GRCGYC 3 cut(s) 281, 426, 1176
BssSI CACGAG 1 cut(s) 537
Bst2BI CACGAG 1 cut(s) 537
Bst2UI CCWGG 3 cut(s) 315, 446, 526
Bst4CI ACNGT 5 cut(s) 43, 91, 119, 625, 1018
BstACI GRCGYC 3 cut(s) 281, 426, 1176
BstAPI GCANNNNNTGC 1 cut(s) 332
BstBAI YACGTR 1 cut(s) 516
BstBI TTCGAA 2 cut(s) 196, 1187
BstC8I GCNNGC 4 cut(s) 170, 477, 681, 1180
BstDEI CTNAG 6 cut(s) 24, 239, 389, 764, 1110, 1132
BstEII GGTNACC 1 cut(s) 637
BstF5I GGATG 7 cut(s) 337, 514, 561, 811, 925, 1012, 1016
BstFNI CGCG 8 cut(s) 155, 278, 364, 366, 666, 692, 744, 1169
BstH2I RGCGCY 3 cut(s) 182, 389, 1179
BstHHI GCGC 5 cut(s) 181, 366, 388, 1169, 1178
BstKTI GATC 5 cut(s) 148, 531, 664, 690, 799
BstMAI GTCTC 2 cut(s) 668, 837
BstMBI GATC 5 cut(s) 145, 528, 661, 687, 796
BstMCI CGRYCG 5 cut(s) 121, 144, 209, 362, 846
BstNI CCWGG 3 cut(s) 315, 446, 526
BstNSI RCATGY 1 cut(s) 1384
BstPAI GACNNNNGTC 2 cut(s) 595, 759
BstPI GGTNACC 1 cut(s) 637
BstSCI CCNGG 8 cut(s) 163, 262, 313, 444, 524, 649, 650, 710
BstSFI CTRYAG 1 cut(s) 806
BstSLI GKGCMC 1 cut(s) 455
BstUI CGCG 8 cut(s) 155, 278, 364, 366, 666, 692, 744, 1169
BstV1I GCAGC 7 cut(s) 342, 605, 779, 792, 793, 820, 854
BstV2I GAAGAC 2 cut(s) 118, 152
BsuRI GGCC 3 cut(s) 168, 453, 524
BtgZI GCGATG 1 cut(s) 915
BtrI CACGTC 2 cut(s) 226, 250
BtsCI GGATG 7 cut(s) 337, 514, 561, 811, 925, 1012, 1016
BtsI GCAGTG 2 cut(s) 333, 418
BtsIMutI CAGTG 3 cut(s) 333, 418, 1140
BtuMI TCGCGA 1 cut(s) 744
Cac8I GCNNGC 4 cut(s) 170, 477, 681, 1180
CaiI CAGNNNCTG 1 cut(s) 805
CfoI GCGC 5 cut(s) 181, 366, 388, 1169, 1178
Cfr10I RCCGGY 1 cut(s) 723
Cfr13I GGNCC 4 cut(s) 451, 452, 590, 772
Cfr9I CCCGGG 1 cut(s) 650
CseI GACGC 3 cut(s) 361, 364, 685
Csp6I GTAC 4 cut(s) 53, 307, 519, 940
CspCI CAANNNNNGTGG 2 cut(s) 1355, 1390
CviAII CATG 1 cut(s) 1381
CviQI GTAC 4 cut(s) 53, 307, 519, 940
DdeI CTNAG 6 cut(s) 24, 239, 389, 764, 1110, 1132
DinI GGCGCC 1 cut(s) 1177
DpnI GATC 5 cut(s) 147, 530, 663, 689, 798
DpnII GATC 5 cut(s) 145, 528, 661, 687, 796
DraIII CACNNNGTG 1 cut(s) 229
DrdI GACNNNNNNGTC 2 cut(s) 122, 358
DriI GACNNNNNGTC 2 cut(s) 250, 607
DseDI GACNNNNNNGTC 2 cut(s) 122, 358
EaeI YGGCCR 2 cut(s) 166, 522
Eam1105I GACNNNNNGTC 2 cut(s) 250, 607
Ecl136II GAGCTC 1 cut(s) 1116
Eco24I GRGCYC 2 cut(s) 455, 1118
Eco47I GGWCC 2 cut(s) 590, 772
Eco53kI GAGCTC 1 cut(s) 1116
Eco57I CTGAAG 1 cut(s) 357
Eco72I CACGTG 1 cut(s) 516
Eco88I CYCGRG 2 cut(s) 650, 718
Eco91I GGTNACC 1 cut(s) 637
EcoICRI GAGCTC 1 cut(s) 1116
EcoO109I RGGNCCY 2 cut(s) 452, 772
EcoO65I GGTNACC 1 cut(s) 637
EcoRI GAATTC 2 cut(s) 1078, 1332
EcoRII CCWGG 3 cut(s) 313, 444, 524
EcoT22I ATGCAT 1 cut(s) 68
EcoT38I GRGCYC 2 cut(s) 455, 1118
EgeI GGCGCC 1 cut(s) 1177
EheI GGCGCC 1 cut(s) 1177
Esp3I CGTCTC 1 cut(s) 668
FaeI CATG 1 cut(s) 1384
FaiI YATR 9 cut(s) 68, 533, 607, 1158, 1303, 1350, 1359, 1382, 1408
FaqI GGGAC 4 cut(s) 407, 603, 785, 1078
FatI CATG 1 cut(s) 1380
FauI CCCGC 4 cut(s) 463, 1034, 1064, 1134
FokI GGATG 7 cut(s) 344, 521, 568, 798, 932, 1019, 1023
FriOI GRGCYC 2 cut(s) 455, 1118
FspBI CTAG 2 cut(s) 954, 1119
GlaI GCGC 5 cut(s) 180, 365, 387, 1168, 1177
GsuI CTGGAG 2 cut(s) 194, 203
HaeII RGCGCY 3 cut(s) 182, 389, 1179
HaeIII GGCC 3 cut(s) 168, 453, 524
HapII CCGG 9 cut(s) 165, 205, 263, 563, 593, 642, 651, 712, 724
HgaI GACGC 3 cut(s) 361, 364, 685
HhaI GCGC 5 cut(s) 181, 366, 388, 1169, 1178
Hin1I GRCGYC 3 cut(s) 281, 426, 1176
Hin1II CATG 1 cut(s) 1384
Hin6I GCGC 5 cut(s) 179, 364, 386, 1167, 1176
HinP1I GCGC 5 cut(s) 179, 364, 386, 1167, 1176
HinfI GANTC 3 cut(s) 596, 854, 1001
HpaII CCGG 9 cut(s) 165, 205, 263, 563, 593, 642, 651, 712, 724
HphI GGTGA 4 cut(s) 210, 649, 852, 1033
Hpy166II GTNNAC 4 cut(s) 343, 400, 418, 519
Hpy188III TCNNGA 7 cut(s) 35, 229, 631, 718, 743, 752, 875
Hpy8I GTNNAC 4 cut(s) 343, 400, 418, 519
HpyAV CCTTC 4 cut(s) 66, 400, 1055, 1224
HpyCH4III ACNGT 5 cut(s) 43, 91, 119, 625, 1018
HpyCH4IV ACGT 8 cut(s) 225, 249, 281, 396, 426, 515, 603, 912
HpyCH4V TGCA 3 cut(s) 66, 621, 808
HpyF3I CTNAG 6 cut(s) 24, 239, 389, 764, 1110, 1132
HpySE526I ACGT 8 cut(s) 225, 249, 281, 396, 426, 515, 603, 912
Hsp92I GRCGYC 3 cut(s) 281, 426, 1176
Hsp92II CATG 1 cut(s) 1384
HspAI GCGC 5 cut(s) 179, 364, 386, 1167, 1176
KasI GGCGCC 1 cut(s) 1175
KflI GGGWCCC 1 cut(s) 772
Kzo9I GATC 5 cut(s) 145, 528, 661, 687, 796
LmnI GCTCC 5 cut(s) 267, 789, 986, 1138, 1377
Lsp1109I GCAGC 7 cut(s) 342, 605, 779, 792, 793, 820, 854
LweI GCATC 3 cut(s) 105, 322, 820
MaeI CTAG 2 cut(s) 954, 1119
MaeII ACGT 8 cut(s) 225, 249, 281, 396, 426, 515, 603, 912
MaeIII GTNAC 3 cut(s) 233, 637, 858
MalI GATC 5 cut(s) 147, 530, 663, 689, 798
MbiI CCGCTC 3 cut(s) 270, 989, 1141
MboI GATC 5 cut(s) 145, 528, 661, 687, 796
MboII GAAGA 3 cut(s) 118, 152, 1070
MhlI GDGCHC 2 cut(s) 455, 1118
MluCI AATT 9 cut(s) 192, 198, 963, 971, 1027, 1078, 1100, 1325, 1332
Mly113I GGCGCC 1 cut(s) 1176
MlyI GAGTC 2 cut(s) 605, 848
MmeI TCCRAC 3 cut(s) 486, 624, 711
Mph1103I ATGCAT 1 cut(s) 68
MseI TTAA 5 cut(s) 462, 915, 1149, 1320, 1388
MslI CAYNNNNRTG 1 cut(s) 326
MspA1I CMGCKG 3 cut(s) 172, 784, 1182
MspI CCGG 9 cut(s) 165, 205, 263, 563, 593, 642, 651, 712, 724
MspR9I CCNGG 8 cut(s) 165, 264, 315, 446, 526, 651, 652, 712
Mva1269I GAATGC 1 cut(s) 28
MvaI CCWGG 3 cut(s) 315, 446, 526
MvnI CGCG 8 cut(s) 155, 278, 364, 366, 666, 692, 744, 1169
NarI GGCGCC 1 cut(s) 1176
NciI CCSGG 5 cut(s) 165, 264, 651, 652, 712
NdeII GATC 5 cut(s) 145, 528, 661, 687, 796
NlaIII CATG 1 cut(s) 1384
NmuCI GTSAC 2 cut(s) 637, 858
NruI TCGCGA 1 cut(s) 744
NsiI ATGCAT 1 cut(s) 68
NspI RCATGY 1 cut(s) 1384
NspV TTCGAA 2 cut(s) 196, 1187
OliI CACNNNNGTG 1 cut(s) 326
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 512
PctI GAATGC 1 cut(s) 28
PfeI GAWTC 1 cut(s) 1001
PleI GAGTC 2 cut(s) 604, 848
PluTI GGCGCC 1 cut(s) 1179
PmaCI CACGTG 1 cut(s) 516
PmlI CACGTG 1 cut(s) 516
PpsI GAGTC 2 cut(s) 604, 848
Ppu21I YACGTR 1 cut(s) 516
PpuMI RGGWCCY 1 cut(s) 772
PshAI GACNNNNGTC 2 cut(s) 595, 759
Psp124BI GAGCTC 1 cut(s) 1118
Psp5II RGGWCCY 1 cut(s) 772
Psp6I CCWGG 3 cut(s) 313, 444, 524
PspCI CACGTG 1 cut(s) 516
PspEI GGTNACC 1 cut(s) 637
PspGI CCWGG 3 cut(s) 313, 444, 524
PspOMI GGGCCC 1 cut(s) 451
PspPI GGNCC 4 cut(s) 451, 452, 590, 772
PspPPI RGGWCCY 1 cut(s) 772
PstI CTGCAG 1 cut(s) 810
PstNI CAGNNNCTG 1 cut(s) 805
PvuII CAGCTG 1 cut(s) 172
RruI TCGCGA 1 cut(s) 744
RsaI GTAC 4 cut(s) 54, 308, 520, 941
RsaNI GTAC 4 cut(s) 53, 307, 519, 940
RseI CAYNNNNRTG 1 cut(s) 326
SacI GAGCTC 1 cut(s) 1118
SaqAI TTAA 5 cut(s) 462, 915, 1149, 1320, 1388
Sau3AI GATC 5 cut(s) 145, 528, 661, 687, 796
Sau96I GGNCC 4 cut(s) 451, 452, 590, 772
ScaI AGTACT 1 cut(s) 308
SchI GAGTC 2 cut(s) 605, 848
ScrFI CCNGG 8 cut(s) 165, 264, 315, 446, 526, 651, 652, 712
SduI GDGCHC 2 cut(s) 455, 1118
SfaNI GCATC 3 cut(s) 105, 322, 820
SfcI CTRYAG 1 cut(s) 806
SfoI GGCGCC 1 cut(s) 1177
SfuI TTCGAA 2 cut(s) 196, 1187
SinI GGWCC 2 cut(s) 590, 772
SmaI CCCGGG 1 cut(s) 652
SmiMI CAYNNNNRTG 1 cut(s) 326
Sse9I AATT 9 cut(s) 192, 198, 963, 971, 1027, 1078, 1100, 1325, 1332
SspDI GGCGCC 1 cut(s) 1175
SspMI CTAG 2 cut(s) 954, 1119
SstI GAGCTC 1 cut(s) 1118
StyD4I CCNGG 8 cut(s) 163, 262, 313, 444, 524, 649, 650, 710
TaaI ACNGT 5 cut(s) 43, 91, 119, 625, 1018
TaiI ACGT 8 cut(s) 228, 252, 284, 399, 429, 518, 606, 915
TaqI TCGA 7 cut(s) 144, 196, 404, 630, 753, 876, 1187
TaqII GACCGA 1 cut(s) 130
TasI AATT 9 cut(s) 192, 198, 963, 971, 1027, 1078, 1100, 1325, 1332
TatI WGTACW 1 cut(s) 306
TauI GCSGC 5 cut(s) 134, 155, 386, 851, 992
TfiI GAWTC 1 cut(s) 1001
Tru1I TTAA 5 cut(s) 462, 915, 1149, 1320, 1388
Tru9I TTAA 5 cut(s) 462, 915, 1149, 1320, 1388
TscAI CASTG 3 cut(s) 333, 425, 1140
TseFI GTSAC 2 cut(s) 637, 858
TseI GCWGC 7 cut(s) 355, 618, 781, 792, 805, 808, 867
Tsp45I GTSAC 2 cut(s) 637, 858
TspDTI ATGAA 3 cut(s) 26, 204, 918
TspMI CCCGGG 1 cut(s) 650
TspRI CASTG 3 cut(s) 333, 425, 1140
VpaK11BI GGWCC 2 cut(s) 590, 772
XapI RAATTY 3 cut(s) 192, 1078, 1332
XceI RCATGY 1 cut(s) 1384
XmaI CCCGGG 1 cut(s) 650
XspI CTAG 2 cut(s) 954, 1119
ZraI GACGTC 2 cut(s) 282, 427
ZrmI AGTACT 1 cut(s) 308
Zsp2I ATGCAT 1 cut(s) 68
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.