Rw5G042920
ERF Family

Belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0015235
Physical Location & Seq
Forward (+)
75066522 .. 75067915
1394 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G042920.1

Sequence Viewer

Length: 1362 bp
ATGGAGAATTCTCACTATAGTCAATCCCACACGGCTTCATGGATCAGAGGCAAGTGCGTAGGCAGAGGCGCGTTCGGCACGGTCAATATCGGAGTCCGCAAATCGGACGGTGAAGTGTTCGCCGTCAAGTCCGTGGATCTCGCCGCGTGTCTCCCCAGCCAGGCGGAGGCGCTCGAGAACGAAATCCGGATTCTCCGGTCGCTCTCCTCGCCTTACGTCGTGAAGTACCTGAACGACGATCACACGGCGTCGTTTCGGAACTTGCATATGGAGTACTTGCCGGGCGGCACCGCCGGGGACTCTGCTGACGTGGACGAGAGTGTTGTACAGTCGCGGACGTGGTGCGTGGTGTCGGCTCTCAAGTATGTACACTCCCGGGGGATTGTTCACTGCGACGTGAAAGGGAGAAATGTTCTGGTGGGGCCCGGTTCGGACCAGGCGAAGCTCGCGGATTTCGGTTCGGCCGTAGATTATCTGTCGGGGGACGGGTGTAGGGGGAGAATCTCGCCACGTGGAAGCCCGCTGTGGATGGCGCCGGAGGTGATTAGCGGGGAGTATCAGGGGCCCGAGTCCGACGTGTGGTCGTTGGGTTGCACGGTGATCGAGATGGTCACGGGAAAACCGGCGTGGGAGGACGAGGGAATGGAGACGCTGAGCCGGATCGGGTTGTCGGGCGGGTTGCCGGAGTTTCCGACCCGGTTGTCCGAAACGGACCCGGAGGAGAGGTGGAGCTGCGATCAGCTACTGCAGCACCCATTTCTGGCGTCAGTTTCGCCCAATGCGGCGGCGGATTCTTCGCCGCGGTGCGTGCTCGACTGGGTCAACTCGGAGTTCGAAGACGACGAGTACGAAAACGACGAAGAGAGTTGTTCTATTCACGAGGTTTCCGCTAAGGAGCGGATTGGTAAATTGGCCTCGAACTCAGGGGTAGATTGGGAGTTTTCGGACGGGTGGACGGTGGTGCGGGGCGCAGTAGAAGTAGTGGAATCCGGCGCGTTTTCGCGGGAAGAGACGAGTTCGGGATATTCAGATTGTGAAGGGGCATTTTCGGAAGGTGAAAGTTGCGGTGGCGGTGATTTGGTAGAGTGGGCACGTAATGAGTGTGGTACGGTTGGCTCAAGTGGTCGTTGGTATGGGCCGTCGGTGATGGAATATTCGGCGGTGGAGAGGAGCGTCGGCAAAGGAAGAAAGTCTTATAATTATTATAGCCGGTGTAGTAATTTGTGTAGAAGCAGATTTTTACTTGCATATTCTTTTGGGGTGAAAATGAATAAATTATTGATTTTTATGAGTTGTTATACTTGTATTATCTTTATTTGGAGATATAATAAAGTCAAAATATTACTTGATTTTTCTTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000165 GO:0000186 GO:0000746 GO:0000747 GO:0000749 GO:0000750 GO:0000935 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007186 GO:0008150 GO:0008152 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010514 GO:0010562 GO:0010564 GO:0010604 GO:0010646 GO:0010647 GO:0010941 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019236 GO:0019538 GO:0019953 GO:0022414 GO:0022603 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030428 GO:0031098 GO:0031136 GO:0031137 GO:0031139 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032005 GO:0032147 GO:0032153 GO:0032155 GO:0032268 GO:0032270 GO:0033554 GO:0033674 GO:0034306 GO:0034307 GO:0035556 GO:0036211 GO:0042173 GO:0042221 GO:0042325 GO:0042327 GO:0042981 GO:0043067 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0043902 GO:0043937 GO:0043940 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044703 GO:0044764 GO:0045595 GO:0045859 GO:0045860 GO:0045937 GO:0046999 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051286 GO:0051338 GO:0051347 GO:0051445 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071444 GO:0071507 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0140096 GO:1901564 GO:1902531 GO:1902533 GO:2000241 GO:2000243
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

453

Amino Acids

49.71

Weight (kDa)

4.88

Isoelectric Point (pI)

46.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 17 - 254 5.9e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 18 - 211 6.4e-23 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015235)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07150 AT1G07150 AT2G30040
fragaria_vesca FvH4_3g39010
malus_domestica MD03G1065400.v1.1 MD11G1070200.v1.1
prunus_persica Prupe.6G054400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0070321
rosa_laevigata RLG00000036124
rosa_multiflora Rmu_co8495105.1_g000001 Rmu_sc0009590.1_g000003
rosa_rugosa Rorug05G0404900
rosa_samantha Rh5AG460500 Rh5BG478800 Rh5CG503000
rosa_wichuraiana Rw5G042920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1197
AasI GACNNNNNNGTC 1 cut(s) 700
AccB1I GGYRCC 2 cut(s) 287, 532
AccBSI CCGCTC 1 cut(s) 898
AccII CGCG 7 cut(s) 71, 146, 334, 449, 802, 995, 1003
AccIII TCCGGA 1 cut(s) 186
AclWI GGATC 3 cut(s) 50, 144, 668
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 1 cut(s) 7
AcvI CACGTG 1 cut(s) 512
AcyI GRCGYC 3 cut(s) 248, 533, 764
AfaI GTAC 6 cut(s) 227, 275, 327, 369, 848, 1108
AfiI CCNNNNNNNGG 5 cut(s) 103, 160, 166, 579, 760
AflIII ACRYGT 1 cut(s) 576
AhdI GACNNNNNGTC 1 cut(s) 580
AjiI CACGTC 4 cut(s) 310, 339, 397, 577
AjnI CCWGG 2 cut(s) 159, 435
AluBI AGCT 3 cut(s) 445, 732, 742
AluI AGCT 3 cut(s) 445, 732, 742
Alw21I GWGCWC 1 cut(s) 813
Alw26I GTCTC 3 cut(s) 155, 641, 1004
AlwI GGATC 3 cut(s) 50, 144, 668
AlwNI CAGNNNCTG 1 cut(s) 745
Ama87I CYCGRG 3 cut(s) 173, 375, 566
Aor13HI TCCGGA 1 cut(s) 186
AoxI GGCC 5 cut(s) 422, 462, 563, 912, 1136
ApaI GGGCCC 2 cut(s) 426, 567
ApeKI GCWGC 2 cut(s) 732, 748
ApoI RAATTY 1 cut(s) 7
AspLEI GCGC 5 cut(s) 71, 172, 535, 971, 995
AspS9I GGNCC 7 cut(s) 422, 423, 433, 563, 564, 712, 1136
AsuC2I CCSGG 7 cut(s) 282, 295, 376, 377, 426, 697, 716
AsuHPI GGTGA 7 cut(s) 122, 553, 610, 1067, 1085, 1156, 1273
AsuII TTCGAA 1 cut(s) 834
AvaI CYCGRG 3 cut(s) 173, 375, 566
AvaII GGWCC 2 cut(s) 433, 712
BaeGI GKGCMC 3 cut(s) 426, 567, 1093
BanI GGYRCC 2 cut(s) 287, 532
BanII GRGCYC 2 cut(s) 426, 567
BauI CACGAG 1 cut(s) 878
BbrPI CACGTG 1 cut(s) 512
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 813
BbvI GCAGC 2 cut(s) 719, 760
BccI CCATC 3 cut(s) 523, 601, 1141
BceAI ACGGC 5 cut(s) 48, 107, 261, 449, 1123
BcgI CGANNNNNNTGC 2 cut(s) 583, 617
BciT130I CCWGG 2 cut(s) 161, 437
BcnI CCSGG 7 cut(s) 282, 295, 376, 377, 426, 697, 716
BcoDI GTCTC 3 cut(s) 155, 641, 1004
BfmI CTRYAG 2 cut(s) 16, 746
BfoI RGCGCY 2 cut(s) 173, 536
BisI GCNGC 7 cut(s) 144, 286, 733, 749, 783, 786, 800
BlpI GCTNAGC 1 cut(s) 653
BlsI GCNGC 7 cut(s) 145, 287, 734, 750, 784, 787, 801
BmcAI AGTACT 1 cut(s) 275
Bme1390I CCNGG 9 cut(s) 161, 282, 295, 376, 377, 426, 437, 697, 716
Bme18I GGWCC 2 cut(s) 433, 712
BmeRI GACNNNNNGTC 1 cut(s) 580
BmeT110I CYCGRG 3 cut(s) 173, 375, 566
BmgBI CACGTC 4 cut(s) 310, 339, 397, 577
BmgT120I GGNCC 7 cut(s) 422, 423, 433, 563, 564, 712, 1136
BmiI GGNNCC 7 cut(s) 289, 423, 424, 534, 564, 565, 714
BmrFI CCNGG 9 cut(s) 161, 282, 295, 376, 377, 426, 437, 697, 716
BmrI ACTGGG 1 cut(s) 826
BmuI ACTGGG 1 cut(s) 826
BpiI GAAGAC 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 891
Bpu1102I GCTNAGC 1 cut(s) 653
Bpu14I TTCGAA 1 cut(s) 834
BpuEI CTTGAG 2 cut(s) 344, 1102
BpuMI CCSGG 7 cut(s) 282, 295, 376, 377, 426, 697, 716
BsaAI YACGTR 2 cut(s) 512, 1094
BsaHI GRCGYC 3 cut(s) 248, 533, 764
BsaJI CCNNGG 5 cut(s) 132, 294, 375, 376, 800
BsaWI WCCGGW 2 cut(s) 186, 195
BsaXI ACNNNNNCTCC 2 cut(s) 710, 740
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 160, 166, 579, 760
Bse118I RCCGGY 2 cut(s) 622, 1209
Bse1I ACTGG 1 cut(s) 821
BseAI TCCGGA 1 cut(s) 186
BseBI CCWGG 2 cut(s) 161, 437
BseDI CCNNGG 5 cut(s) 132, 294, 375, 376, 800
BseGI GGATG 1 cut(s) 534
BseLI CCNNNNNNNGG 5 cut(s) 103, 160, 166, 579, 760
BseMII CTCAG 2 cut(s) 644, 936
BseNI ACTGG 1 cut(s) 821
BseRI GAGGAG 3 cut(s) 196, 734, 1183
BseSI GKGCMC 3 cut(s) 426, 567, 1093
BseX3I CGGCCG 1 cut(s) 462
BseXI GCAGC 2 cut(s) 719, 760
BseYI CCCAGC 1 cut(s) 155
Bsh1236I CGCG 7 cut(s) 71, 146, 334, 449, 802, 995, 1003
Bsh1285I CGRYCG 2 cut(s) 200, 465
BshFI GGCC 5 cut(s) 424, 464, 565, 914, 1138
BshNI GGYRCC 2 cut(s) 287, 532
BsiEI CGRYCG 2 cut(s) 200, 465
BsiHKAI GWGCWC 1 cut(s) 813
BsiHKCI CYCGRG 3 cut(s) 173, 375, 566
BslFI GGGAC 2 cut(s) 311, 497
BslI CCNNNNNNNGG 5 cut(s) 103, 160, 166, 579, 760
BsmAI GTCTC 3 cut(s) 155, 641, 1004
BsmBI CGTCTC 2 cut(s) 641, 1004
BsmFI GGGAC 2 cut(s) 311, 497
BsnI GGCC 5 cut(s) 424, 464, 565, 914, 1138
BsoBI CYCGRG 3 cut(s) 173, 375, 566
Bsp119I TTCGAA 1 cut(s) 834
Bsp120I GGGCCC 2 cut(s) 422, 563
Bsp1286I GDGCHC 4 cut(s) 426, 567, 813, 1093
Bsp13I TCCGGA 1 cut(s) 186
Bsp1407I TGTACA 2 cut(s) 325, 367
Bsp143I GATC 6 cut(s) 42, 136, 238, 600, 660, 736
Bsp1720I GCTNAGC 1 cut(s) 653
BspANI GGCC 5 cut(s) 424, 464, 565, 914, 1138
BspCNI CTCAG 2 cut(s) 645, 935
BspEI TCCGGA 1 cut(s) 186
BspFNI CGCG 7 cut(s) 71, 146, 334, 449, 802, 995, 1003
BspLI GGNNCC 7 cut(s) 289, 423, 424, 534, 564, 565, 714
BspMAI CTGCAG 1 cut(s) 750
BspPI GGATC 3 cut(s) 50, 144, 668
BspT104I TTCGAA 1 cut(s) 834
BspT107I GGYRCC 2 cut(s) 287, 532
BsrBI CCGCTC 1 cut(s) 898
BsrFI RCCGGY 2 cut(s) 622, 1209
BsrGI TGTACA 2 cut(s) 325, 367
BsrI ACTGG 1 cut(s) 821
BssAI RCCGGY 2 cut(s) 622, 1209
BssECI CCNNGG 5 cut(s) 132, 294, 375, 376, 800
BssMI GATC 6 cut(s) 42, 136, 238, 600, 660, 736
BssNI GRCGYC 3 cut(s) 248, 533, 764
BssSI CACGAG 1 cut(s) 878
Bst2BI CACGAG 1 cut(s) 878
Bst2UI CCWGG 2 cut(s) 161, 437
Bst4CI ACNGT 6 cut(s) 82, 110, 330, 598, 958, 1111
Bst6I CTCTTC 2 cut(s) 855, 1002
BstACI GRCGYC 3 cut(s) 248, 533, 764
BstAUI TGTACA 2 cut(s) 325, 367
BstBAI YACGTR 2 cut(s) 512, 1094
BstBI TTCGAA 1 cut(s) 834
BstC8I GCNNGC 3 cut(s) 447, 521, 809
BstDEI CTNAG 3 cut(s) 653, 891, 922
BstDSI CCRYGG 2 cut(s) 132, 800
BstF5I GGATG 1 cut(s) 534
BstFNI CGCG 7 cut(s) 71, 146, 334, 449, 802, 995, 1003
BstH2I RGCGCY 2 cut(s) 173, 536
BstHHI GCGC 5 cut(s) 71, 172, 535, 971, 995
BstKTI GATC 6 cut(s) 45, 139, 241, 603, 663, 739
BstMAI GTCTC 3 cut(s) 155, 641, 1004
BstMBI GATC 6 cut(s) 42, 136, 238, 600, 660, 736
BstMCI CGRYCG 2 cut(s) 200, 465
BstMWI GCNNNNNNNGC 5 cut(s) 75, 208, 446, 748, 808
BstNI CCWGG 2 cut(s) 161, 437
BstSCI CCNGG 9 cut(s) 159, 280, 293, 374, 375, 424, 435, 695, 714
BstSFI CTRYAG 2 cut(s) 16, 746
BstSLI GKGCMC 3 cut(s) 426, 567, 1093
BstUI CGCG 7 cut(s) 71, 146, 334, 449, 802, 995, 1003
BstV1I GCAGC 2 cut(s) 719, 760
BstV2I GAAGAC 1 cut(s) 843
BstX2I RGATCY 1 cut(s) 136
BstYI RGATCY 1 cut(s) 136
BstZI CGGCCG 1 cut(s) 462
BsuRI GGCC 5 cut(s) 424, 464, 565, 914, 1138
BtgI CCRYGG 2 cut(s) 132, 800
BtrI CACGTC 4 cut(s) 310, 339, 397, 577
BtsCI GGATG 1 cut(s) 534
BtsI GCAGTG 1 cut(s) 388
BtsIMutI CAGTG 1 cut(s) 388
Cac8I GCNNGC 3 cut(s) 447, 521, 809
CaiI CAGNNNCTG 1 cut(s) 745
CfoI GCGC 5 cut(s) 71, 172, 535, 971, 995
Cfr10I RCCGGY 2 cut(s) 622, 1209
Cfr13I GGNCC 7 cut(s) 422, 423, 433, 563, 564, 712, 1136
Cfr42I CCGCGG 1 cut(s) 803
Cfr9I CCCGGG 1 cut(s) 375
CseI GACGC 4 cut(s) 237, 658, 753, 1162
Csp6I GTAC 6 cut(s) 226, 274, 326, 368, 847, 1107
CviAII CATG 1 cut(s) 39
CviQI GTAC 6 cut(s) 226, 274, 326, 368, 847, 1107
DdeI CTNAG 3 cut(s) 653, 891, 922
DinI GGCGCC 1 cut(s) 534
DpnI GATC 6 cut(s) 44, 138, 240, 602, 662, 738
DpnII GATC 6 cut(s) 42, 136, 238, 600, 660, 736
DrdI GACNNNNNNGTC 1 cut(s) 700
DriI GACNNNNNGTC 1 cut(s) 580
DseDI GACNNNNNNGTC 1 cut(s) 700
EaeI YGGCCR 1 cut(s) 462
EagI CGGCCG 1 cut(s) 462
Eam1104I CTCTTC 2 cut(s) 855, 1002
Eam1105I GACNNNNNGTC 1 cut(s) 580
EarI CTCTTC 2 cut(s) 855, 1002
EciI GGCGGA 2 cut(s) 179, 803
EclXI CGGCCG 1 cut(s) 462
Eco24I GRGCYC 2 cut(s) 426, 567
Eco47I GGWCC 2 cut(s) 433, 712
Eco52I CGGCCG 1 cut(s) 462
Eco72I CACGTG 1 cut(s) 512
Eco88I CYCGRG 3 cut(s) 173, 375, 566
EcoO109I RGGNCCY 2 cut(s) 422, 563
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 2 cut(s) 159, 435
EcoT38I GRGCYC 2 cut(s) 426, 567
EgeI GGCGCC 1 cut(s) 534
EheI GGCGCC 1 cut(s) 534
Esp3I CGTCTC 2 cut(s) 641, 1004
FaeI CATG 1 cut(s) 42
FalI AAGNNNNNCTT 2 cut(s) 1177, 1209
FaqI GGGAC 2 cut(s) 311, 497
FatI CATG 1 cut(s) 38
FauI CCCGC 5 cut(s) 528, 542, 668, 957, 996
FauNDI CATATG 1 cut(s) 267
Fnu4HI GCNGC 7 cut(s) 144, 286, 733, 749, 783, 786, 800
FokI GGATG 1 cut(s) 541
FriOI GRGCYC 2 cut(s) 426, 567
Fsp4HI GCNGC 7 cut(s) 144, 286, 733, 749, 783, 786, 800
GlaI GCGC 5 cut(s) 70, 171, 534, 970, 994
GluI GCNGC 7 cut(s) 144, 286, 733, 749, 783, 786, 800
GsaI CCCAGC 1 cut(s) 159
HaeII RGCGCY 2 cut(s) 173, 536
HaeIII GGCC 5 cut(s) 424, 464, 565, 914, 1138
HgaI GACGC 4 cut(s) 237, 658, 753, 1162
HhaI GCGC 5 cut(s) 71, 172, 535, 971, 995
Hin1I GRCGYC 3 cut(s) 248, 533, 764
Hin1II CATG 1 cut(s) 42
Hin6I GCGC 5 cut(s) 69, 170, 533, 969, 993
HinP1I GCGC 5 cut(s) 69, 170, 533, 969, 993
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HinfI GANTC 7 cut(s) 93, 190, 299, 501, 569, 791, 986
HphI GGTGA 7 cut(s) 122, 553, 610, 1067, 1085, 1156, 1273
Hpy166II GTNNAC 5 cut(s) 313, 370, 388, 823, 954
Hpy188III TCNNGA 6 cut(s) 175, 187, 220, 604, 878, 1020
Hpy8I GTNNAC 5 cut(s) 313, 370, 388, 823, 954
Hpy99I CGWCG 9 cut(s) 221, 239, 253, 398, 578, 845, 860, 1144, 1178
HpyAV CCTTC 2 cut(s) 1031, 1046
HpyCH4III ACNGT 6 cut(s) 82, 110, 330, 598, 958, 1111
HpyCH4IV ACGT 7 cut(s) 216, 309, 338, 396, 511, 576, 1093
HpyCH4V TGCA 4 cut(s) 265, 594, 748, 1247
HpyF10VI GCNNNNNNNGC 5 cut(s) 75, 208, 446, 748, 808
HpyF3I CTNAG 3 cut(s) 653, 891, 922
HpySE526I ACGT 7 cut(s) 216, 309, 338, 396, 511, 576, 1093
Hsp92I GRCGYC 3 cut(s) 248, 533, 764
Hsp92II CATG 1 cut(s) 42
HspAI GCGC 5 cut(s) 69, 170, 533, 969, 993
KasI GGCGCC 1 cut(s) 532
Kpn2I TCCGGA 1 cut(s) 186
KspI CCGCGG 1 cut(s) 803
Kzo9I GATC 6 cut(s) 42, 136, 238, 600, 660, 736
LmnI GCTCC 3 cut(s) 729, 895, 1170
Lsp1109I GCAGC 2 cut(s) 719, 760
MaeII ACGT 7 cut(s) 216, 309, 338, 396, 511, 576, 1093
MaeIII GTNAC 1 cut(s) 610
MalI GATC 6 cut(s) 44, 138, 240, 602, 662, 738
MbiI CCGCTC 1 cut(s) 898
MboI GATC 6 cut(s) 42, 136, 238, 600, 660, 736
MboII GAAGA 5 cut(s) 786, 848, 872, 1019, 1197
MflI RGATCY 1 cut(s) 136
MhlI GDGCHC 4 cut(s) 426, 567, 813, 1093
MluCI AATT 5 cut(s) 7, 908, 1198, 1219, 1274
Mly113I GGCGCC 1 cut(s) 533
MlyI GAGTC 3 cut(s) 102, 293, 578
MmeI TCCRAC 2 cut(s) 597, 716
MroI TCCGGA 1 cut(s) 186
MspA1I CMGCKG 2 cut(s) 523, 802
MspR9I CCNGG 9 cut(s) 161, 282, 295, 376, 377, 426, 437, 697, 716
MvaI CCWGG 2 cut(s) 161, 437
MvnI CGCG 7 cut(s) 71, 146, 334, 449, 802, 995, 1003
MwoI GCNNNNNNNGC 5 cut(s) 75, 208, 446, 748, 808
NarI GGCGCC 1 cut(s) 533
NciI CCSGG 7 cut(s) 282, 295, 376, 377, 426, 697, 716
NdeI CATATG 1 cut(s) 267
NdeII GATC 6 cut(s) 42, 136, 238, 600, 660, 736
NlaIII CATG 1 cut(s) 42
NlaIV GGNNCC 7 cut(s) 289, 423, 424, 534, 564, 565, 714
NmuCI GTSAC 1 cut(s) 610
NspV TTCGAA 1 cut(s) 834
PaeR7I CTCGAG 1 cut(s) 173
PcsI WCGNNNNNNNCGW 5 cut(s) 437, 462, 840, 846, 855
PfeI GAWTC 4 cut(s) 190, 501, 791, 986
PflFI GACNNNGTC 1 cut(s) 818
PkrI GCNGC 7 cut(s) 145, 287, 734, 750, 784, 787, 801
PleI GAGTC 3 cut(s) 101, 293, 577
PluTI GGCGCC 1 cut(s) 536
PmaCI CACGTG 1 cut(s) 512
PmlI CACGTG 1 cut(s) 512
PpsI GAGTC 3 cut(s) 101, 293, 577
Ppu21I YACGTR 2 cut(s) 512, 1094
PsiI TTATAA 1 cut(s) 1197
Psp6I CCWGG 2 cut(s) 159, 435
PspCI CACGTG 1 cut(s) 512
PspFI CCCAGC 1 cut(s) 155
PspGI CCWGG 2 cut(s) 159, 435
PspN4I GGNNCC 7 cut(s) 289, 423, 424, 534, 564, 565, 714
PspOMI GGGCCC 2 cut(s) 422, 563
PspPI GGNCC 7 cut(s) 422, 423, 433, 563, 564, 712, 1136
PstI CTGCAG 1 cut(s) 750
PstNI CAGNNNCTG 1 cut(s) 745
PsuI RGATCY 1 cut(s) 136
PsyI GACNNNGTC 1 cut(s) 818
RsaI GTAC 6 cut(s) 227, 275, 327, 369, 848, 1108
RsaNI GTAC 6 cut(s) 226, 274, 326, 368, 847, 1107
SacII CCGCGG 1 cut(s) 803
SatI GCNGC 7 cut(s) 144, 286, 733, 749, 783, 786, 800
Sau3AI GATC 6 cut(s) 42, 136, 238, 600, 660, 736
Sau96I GGNCC 7 cut(s) 422, 423, 433, 563, 564, 712, 1136
ScaI AGTACT 1 cut(s) 275
SchI GAGTC 3 cut(s) 102, 293, 578
ScrFI CCNGG 9 cut(s) 161, 282, 295, 376, 377, 426, 437, 697, 716
SduI GDGCHC 4 cut(s) 426, 567, 813, 1093
SfcI CTRYAG 2 cut(s) 16, 746
SfoI GGCGCC 1 cut(s) 534
Sfr274I CTCGAG 1 cut(s) 173
Sfr303I CCGCGG 1 cut(s) 803
SfuI TTCGAA 1 cut(s) 834
SgrBI CCGCGG 1 cut(s) 803
SinI GGWCC 2 cut(s) 433, 712
SlaI CTCGAG 1 cut(s) 173
SmaI CCCGGG 1 cut(s) 377
SmlI CTYRAG 3 cut(s) 173, 359, 1117
SmoI CTYRAG 3 cut(s) 173, 359, 1117
Sse9I AATT 5 cut(s) 7, 908, 1198, 1219, 1274
SspDI GGCGCC 1 cut(s) 532
SspI AATATT 2 cut(s) 1154, 1341
StyD4I CCNGG 9 cut(s) 159, 280, 293, 374, 375, 424, 435, 695, 714
TaaI ACNGT 6 cut(s) 82, 110, 330, 598, 958, 1111
TaiI ACGT 7 cut(s) 219, 312, 341, 399, 514, 579, 1096
TaqI TCGA 5 cut(s) 174, 603, 813, 834, 917
TasI AATT 5 cut(s) 7, 908, 1198, 1219, 1274
TatI WGTACW 3 cut(s) 273, 325, 367
TauI GCSGC 5 cut(s) 146, 288, 785, 788, 802
TfiI GAWTC 4 cut(s) 190, 501, 791, 986
TscAI CASTG 1 cut(s) 395
TseFI GTSAC 1 cut(s) 610
TseI GCWGC 2 cut(s) 732, 748
Tsp45I GTSAC 1 cut(s) 610
TspDTI ATGAA 2 cut(s) 27, 1283
TspGWI ACGGA 2 cut(s) 121, 725
TspMI CCCGGG 1 cut(s) 375
TspRI CASTG 1 cut(s) 395
Tth111I GACNNNGTC 1 cut(s) 818
VpaK11BI GGWCC 2 cut(s) 433, 712
XapI RAATTY 1 cut(s) 7
XhoI CTCGAG 1 cut(s) 173
XmaI CCCGGG 1 cut(s) 375
ZrmI AGTACT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.