AT2G31985

Protein of unknown function (DUF1264)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015191
Physical Location & Seq
Reverse (-)
13613928 .. 13615692
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31985.1

Sequence Viewer

Length: 726 bp
ATGGAGAAAGCAGTTCACTCGTCGACCACGTCTGGACCGGCGGTTCCAGGGGAGACAACAAAGACCGGAACCTCCATAATTGACACTGCAGCCTCCGCTGTTCAAAGTTTTGCACCGGTTAACCAAATCCACCAACATCTCTGCGCGTTCCATTTCTATGCTGACGACATGGCACGACAAGTAGAGGCTCACCATTTCTGCAGTCACGTGAACGAAGAGATGCGTCAGTGTTTGATCTACGACGGTCCGGATGCAAACGCTCGTCTAATTGGATTGGAGTACATAGTGTCAGAGAAGCTGTTCATGACTTTGCCTGACGAAGAGAAGAAGCTTTGGCACTCGCACGAGTGGGAAGTGAAAGGAGGGTTCTTGTTCATGCCTGGTGTTCCAGGTGCCATTCAGCGTAAAGATCTTGATAAAGTCGCTAAGACTTATGGGAAAGTTTTCCATTTTTGGCAAGTTGATTTGGGCCATGAGCTTCCCATTGGTTTACCTAATGTCATGATGGCTGTTACACGCGATGGCCAGCTTTTCCACGAGATGATTCAGGAGGCAGAGAAGCGGTTTGGGGTATCGGTAGAGGGGGAGAGGGACTCACGGGCGTATATGTCTGGACCTGAGCTCGGGATCCATCCATTGGCGAATGGAGGAGGCAAAGGGATGAAACTAGAGCTACGAGAGGTCGATATCAAGCCGGTTGAGTCTGTCGGCAGCGTCTTTGTCTGA

Protein Analysis

241

Amino Acids

26.76

Weight (kDa)

5.69

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1264 PF06884 37 - 203 1.2e-73 Protein of unknown function (DUF1264)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05510 AT2G31985
fragaria_vesca FvH4_2g06270 FvH4_2g06270
malus_domestica MD05G1004300.v1.1
prunus_persica Prupe.8G007500_v2.0.a1
pyrus_communis pycom05g00380
rosa_chinensis RchiOBHm_Chr6g0256911
rosa_laevigata RLG00000014656
rosa_multiflora Rmu_sc0003076.1_g000030
rosa_roxburghii Rroxscaffold_7G00208290
rosa_rugosa Rorug05G0577000
rosa_samantha Rh6AG095700 Rh6BG086500 Rh6DG078500
rosa_wichuraiana Rw6G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 392
AccB7I CCANNNNNTGG 1 cut(s) 637
AccI GTMKAC 1 cut(s) 23
AccII CGCG 2 cut(s) 146, 519
AccIII TCCGGA 1 cut(s) 247
AciI CCGC 3 cut(s) 41, 96, 562
AclWI GGATC 2 cut(s) 622, 635
AcoI YGGCCR 1 cut(s) 523
AcvI CACGTG 1 cut(s) 208
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 2 cut(s) 623, 637
AgeI ACCGGT 1 cut(s) 115
AgsI TTSAA 1 cut(s) 104
AjiI CACGTC 1 cut(s) 30
AjnI CCWGG 3 cut(s) 46, 379, 388
AloI GAACNNNNNNTCC 2 cut(s) 27, 59
AluBI AGCT 6 cut(s) 298, 331, 478, 529, 622, 673
AluI AGCT 6 cut(s) 298, 331, 478, 529, 622, 673
Alw21I GWGCWC 1 cut(s) 624
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 2 cut(s) 622, 635
Ama87I CYCGRG 1 cut(s) 623
Aor13HI TCCGGA 1 cut(s) 247
AoxI GGCC 2 cut(s) 469, 523
ApeKI GCWGC 2 cut(s) 89, 711
AsiGI ACCGGT 1 cut(s) 115
Asp700I GAANNNNTTC 2 cut(s) 299, 443
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 4 cut(s) 35, 245, 469, 614
AsuHPI GGTGA 1 cut(s) 182
AvaI CYCGRG 1 cut(s) 623
AvaII GGWCC 3 cut(s) 35, 245, 614
BalI TGGCCA 1 cut(s) 525
BamHI GGATCC 1 cut(s) 627
BanI GGYRCC 1 cut(s) 392
BanII GRGCYC 1 cut(s) 624
BauI CACGAG 2 cut(s) 344, 536
BbrPI CACGTG 1 cut(s) 208
Bbv12I GWGCWC 1 cut(s) 624
BbvI GCAGC 1 cut(s) 101
BccI CCATC 3 cut(s) 499, 515, 639
BcgI CGANNNNNNTGC 1 cut(s) 34
BciT130I CCWGG 3 cut(s) 48, 381, 390
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 1 cut(s) 668
BfmI CTRYAG 2 cut(s) 87, 199
BglII AGATCT 1 cut(s) 409
BisI GCNGC 2 cut(s) 90, 712
BlsI GCNGC 2 cut(s) 91, 713
Bme1390I CCNGG 3 cut(s) 48, 381, 390
Bme18I GGWCC 3 cut(s) 35, 245, 614
BmeT110I CYCGRG 1 cut(s) 623
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 4 cut(s) 35, 245, 469, 614
BmiI GGNNCC 4 cut(s) 45, 70, 394, 629
BmrFI CCNGG 3 cut(s) 48, 381, 390
BmsI GCATC 2 cut(s) 210, 241
BplI GAGNNNNNCTC 2 cut(s) 578, 610
Bpu10I CCTNAGC 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 208
BsaJI CCNNGG 1 cut(s) 47
BsaWI WCCGGW 3 cut(s) 65, 115, 247
Bsc4I CCNNNNNNNGG 2 cut(s) 623, 637
Bse118I RCCGGY 3 cut(s) 37, 115, 694
BseAI TCCGGA 1 cut(s) 247
BseBI CCWGG 3 cut(s) 48, 381, 390
BseDI CCNNGG 1 cut(s) 47
BseGI GGATG 3 cut(s) 256, 631, 666
BseLI CCNNNNNNNGG 2 cut(s) 623, 637
BseMII CTCAG 1 cut(s) 609
BseRI GAGGAG 1 cut(s) 663
BseXI GCAGC 1 cut(s) 101
Bsh1236I CGCG 2 cut(s) 146, 519
BshFI GGCC 2 cut(s) 471, 525
BshNI GGYRCC 1 cut(s) 392
BshTI ACCGGT 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 624
BsiHKCI CYCGRG 1 cut(s) 623
BsiSI CCGG 5 cut(s) 38, 66, 116, 248, 695
BslFI GGGAC 1 cut(s) 605
BslI CCNNNNNNNGG 2 cut(s) 623, 637
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 605
BsnI GGCC 2 cut(s) 471, 525
BsoBI CYCGRG 1 cut(s) 623
Bsp1286I GDGCHC 1 cut(s) 624
Bsp13I TCCGGA 1 cut(s) 247
Bsp143I GATC 3 cut(s) 234, 409, 627
BspACI CCGC 3 cut(s) 41, 96, 562
BspANI GGCC 2 cut(s) 471, 525
BspCNI CTCAG 1 cut(s) 610
BspEI TCCGGA 1 cut(s) 247
BspFNI CGCG 2 cut(s) 146, 519
BspHI TCATGA 2 cut(s) 303, 501
BspLI GGNNCC 4 cut(s) 45, 70, 394, 629
BspMAI CTGCAG 2 cut(s) 91, 203
BspPI GGATC 2 cut(s) 622, 635
BspT107I GGYRCC 1 cut(s) 392
BsrFI RCCGGY 3 cut(s) 37, 115, 694
BssAI RCCGGY 3 cut(s) 37, 115, 694
BssECI CCNNGG 1 cut(s) 47
BssMI GATC 3 cut(s) 234, 409, 627
BssSI CACGAG 2 cut(s) 344, 536
Bst2BI CACGAG 2 cut(s) 344, 536
Bst2UI CCWGG 3 cut(s) 48, 381, 390
Bst4CI ACNGT 1 cut(s) 245
Bst6I CTCTTC 2 cut(s) 210, 315
BstBAI YACGTR 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 527
BstDEI CTNAG 2 cut(s) 426, 618
BstF5I GGATG 3 cut(s) 256, 631, 666
BstFNI CGCG 2 cut(s) 146, 519
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 3 cut(s) 237, 412, 630
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 3 cut(s) 234, 409, 627
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstNI CCWGG 3 cut(s) 48, 381, 390
BstSCI CCNGG 3 cut(s) 46, 379, 388
BstSFI CTRYAG 2 cut(s) 87, 199
BstUI CGCG 2 cut(s) 146, 519
BstV1I GCAGC 1 cut(s) 101
BstX2I RGATCY 2 cut(s) 409, 627
BstYI RGATCY 2 cut(s) 409, 627
BsuRI GGCC 2 cut(s) 471, 525
BtgZI GCGATG 1 cut(s) 534
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 3 cut(s) 256, 631, 666
BtsI GCAGTG 1 cut(s) 84
BtsIMutI CAGTG 2 cut(s) 84, 233
Cac8I GCNNGC 1 cut(s) 527
CciI TCATGA 2 cut(s) 303, 501
CfoI GCGC 1 cut(s) 146
Cfr10I RCCGGY 3 cut(s) 37, 115, 694
Cfr13I GGNCC 4 cut(s) 35, 245, 469, 614
CpoI CGGWCCG 1 cut(s) 245
CseI GACGC 2 cut(s) 212, 703
Csp6I GTAC 1 cut(s) 280
CspAI ACCGGT 1 cut(s) 115
CspI CGGWCCG 1 cut(s) 245
CviAII CATG 5 cut(s) 169, 304, 376, 473, 502
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 2 cut(s) 426, 618
DpnI GATC 3 cut(s) 236, 411, 629
DpnII GATC 3 cut(s) 234, 409, 627
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 2 cut(s) 210, 315
EarI CTCTTC 2 cut(s) 210, 315
Ecl136II GAGCTC 1 cut(s) 622
Eco24I GRGCYC 1 cut(s) 624
Eco32I GATATC 1 cut(s) 688
Eco47I GGWCC 3 cut(s) 35, 245, 614
Eco53kI GAGCTC 1 cut(s) 622
Eco72I CACGTG 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 623
EcoICRI GAGCTC 1 cut(s) 622
EcoRII CCWGG 3 cut(s) 46, 379, 388
EcoRV GATATC 1 cut(s) 688
EcoT38I GRGCYC 1 cut(s) 624
FaeI CATG 5 cut(s) 172, 307, 379, 476, 505
FaqI GGGAC 1 cut(s) 605
FatI CATG 5 cut(s) 168, 303, 375, 472, 501
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 2 cut(s) 90, 712
FokI GGATG 3 cut(s) 263, 618, 673
FriOI GRGCYC 1 cut(s) 624
Fsp4HI GCNGC 2 cut(s) 90, 712
FspBI CTAG 1 cut(s) 668
GlaI GCGC 1 cut(s) 145
GluI GCNGC 2 cut(s) 90, 712
HaeIII GGCC 2 cut(s) 471, 525
HapII CCGG 5 cut(s) 38, 66, 116, 248, 695
HgaI GACGC 2 cut(s) 212, 703
HhaI GCGC 1 cut(s) 146
Hin1II CATG 5 cut(s) 172, 307, 379, 476, 505
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HincII GTYRAC 2 cut(s) 24, 121
HindII GTYRAC 2 cut(s) 24, 121
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 3 cut(s) 544, 593, 701
HpaI GTTAAC 1 cut(s) 121
HpaII CCGG 5 cut(s) 38, 66, 116, 248, 695
HphI GGTGA 1 cut(s) 182
Hpy166II GTNNAC 5 cut(s) 16, 24, 121, 211, 491
Hpy188I TCNGA 2 cut(s) 292, 725
Hpy188III TCNNGA 8 cut(s) 33, 248, 304, 413, 502, 548, 612, 625
Hpy8I GTNNAC 5 cut(s) 16, 24, 121, 211, 491
Hpy99I CGWCG 2 cut(s) 25, 245
HpyCH4III ACNGT 1 cut(s) 245
HpyCH4IV ACGT 2 cut(s) 29, 207
HpyCH4V TGCA 4 cut(s) 89, 113, 201, 254
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
HpyF3I CTNAG 2 cut(s) 426, 618
HpySE526I ACGT 2 cut(s) 29, 207
Hsp92II CATG 5 cut(s) 172, 307, 379, 476, 505
HspAI GCGC 1 cut(s) 144
Kpn2I TCCGGA 1 cut(s) 247
KspAI GTTAAC 1 cut(s) 121
Kzo9I GATC 3 cut(s) 234, 409, 627
Lsp1109I GCAGC 1 cut(s) 101
LweI GCATC 2 cut(s) 210, 241
MaeI CTAG 1 cut(s) 668
MaeII ACGT 2 cut(s) 29, 207
MaeIII GTNAC 2 cut(s) 203, 511
MalI GATC 3 cut(s) 236, 411, 629
MboI GATC 3 cut(s) 234, 409, 627
MboII GAAGA 3 cut(s) 227, 332, 337
MflI RGATCY 2 cut(s) 409, 627
MhlI GDGCHC 1 cut(s) 624
MlsI TGGCCA 1 cut(s) 525
MluCI AATT 2 cut(s) 78, 267
MluNI TGGCCA 1 cut(s) 525
MlyI GAGTC 2 cut(s) 587, 710
Mox20I TGGCCA 1 cut(s) 525
MroI TCCGGA 1 cut(s) 247
MroXI GAANNNNTTC 2 cut(s) 299, 443
MscI TGGCCA 1 cut(s) 525
MseI TTAA 1 cut(s) 120
MslI CAYNNNNRTG 1 cut(s) 156
Msp20I TGGCCA 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 5 cut(s) 38, 66, 116, 248, 695
MspR9I CCNGG 3 cut(s) 48, 381, 390
MvaI CCWGG 3 cut(s) 48, 381, 390
MvnI CGCG 2 cut(s) 146, 519
MwoI GCNNNNNNNGC 1 cut(s) 95
NdeII GATC 3 cut(s) 234, 409, 627
NlaIII CATG 5 cut(s) 172, 307, 379, 476, 505
NlaIV GGNNCC 4 cut(s) 45, 70, 394, 629
NmuCI GTSAC 1 cut(s) 203
PagI TCATGA 2 cut(s) 303, 501
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PdmI GAANNNNTTC 2 cut(s) 299, 443
PfeI GAWTC 1 cut(s) 544
PflFI GACNNNGTC 1 cut(s) 28
PflMI CCANNNNNTGG 1 cut(s) 637
PinAI ACCGGT 1 cut(s) 115
PkrI GCNGC 2 cut(s) 91, 713
PleI GAGTC 2 cut(s) 587, 709
PmaCI CACGTG 1 cut(s) 208
PmlI CACGTG 1 cut(s) 208
PpsI GAGTC 2 cut(s) 587, 709
Ppu21I YACGTR 1 cut(s) 208
Psp124BI GAGCTC 1 cut(s) 624
Psp6I CCWGG 3 cut(s) 46, 379, 388
PspCI CACGTG 1 cut(s) 208
PspGI CCWGG 3 cut(s) 46, 379, 388
PspN4I GGNNCC 4 cut(s) 45, 70, 394, 629
PspPI GGNCC 4 cut(s) 35, 245, 469, 614
PstI CTGCAG 2 cut(s) 91, 203
PsuI RGATCY 2 cut(s) 409, 627
PsyI GACNNNGTC 1 cut(s) 28
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
RseI CAYNNNNRTG 1 cut(s) 156
Rsr2I CGGWCCG 1 cut(s) 245
RsrII CGGWCCG 1 cut(s) 245
SacI GAGCTC 1 cut(s) 624
SalI GTCGAC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 2 cut(s) 90, 712
Sau3AI GATC 3 cut(s) 234, 409, 627
Sau96I GGNCC 4 cut(s) 35, 245, 469, 614
SchI GAGTC 2 cut(s) 587, 710
ScrFI CCNGG 3 cut(s) 48, 381, 390
SduI GDGCHC 1 cut(s) 624
SfaNI GCATC 2 cut(s) 210, 241
SfcI CTRYAG 2 cut(s) 87, 199
SinI GGWCC 3 cut(s) 35, 245, 614
SmiMI CAYNNNNRTG 1 cut(s) 156
Sse9I AATT 2 cut(s) 78, 267
SsiI CCGC 3 cut(s) 41, 96, 562
SspMI CTAG 1 cut(s) 668
SstI GAGCTC 1 cut(s) 624
StyD4I CCNGG 3 cut(s) 46, 379, 388
TaaI ACNGT 1 cut(s) 245
TaiI ACGT 2 cut(s) 32, 210
TaqI TCGA 2 cut(s) 23, 684
TasI AATT 2 cut(s) 78, 267
TatI WGTACW 1 cut(s) 279
TfiI GAWTC 1 cut(s) 544
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 2 cut(s) 91, 233
TseFI GTSAC 1 cut(s) 203
TseI GCWGC 2 cut(s) 89, 711
Tsp45I GTSAC 1 cut(s) 203
TspDTI ATGAA 3 cut(s) 292, 364, 677
TspRI CASTG 2 cut(s) 91, 233
Tth111I GACNNNGTC 1 cut(s) 28
Van91I CCANNNNNTGG 1 cut(s) 637
VpaK11BI GGWCC 3 cut(s) 35, 245, 614
XmiI GTMKAC 1 cut(s) 23
XmnI GAANNNNTTC 2 cut(s) 299, 443
XspI CTAG 1 cut(s) 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.