FvH4_2g06270

Protein of unknown function (DUF1264)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0015191
Physical Location & Seq
Reverse (-)
5198638 .. 5199843
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06270.t2

Sequence Viewer

Length: 705 bp
ATGGCGACTACTTTCACAGGAGTCCCCGGTGAGCCAACCCAGACCGGAACAGCCATGCTCGAAACCGCAACCGCCGCCATCCAAGACTTCGGCCCCGTCAACAAGGTCCACCAGCACCTCTGCGCCTTCCACTTCTACGCGCACGACATGACACGTCAGGTCGAAGCTCACCATTACTGCGCGCACCAGAATGAGGAGGTGCGTCAGTGCCTAATCTACGACAGCCCCGAGGCCGATGCTAAGCTCATCGGCCTCGAGTACCTCGTCTCGGAGAGACTCTTCTTGACTCTCCCCGATGATGAGAAGAAGCTGTGGCACTCTCACGAGTATGAGGTCAAGAGTGGGGTTTTGTTCATGCCCGGTTTGCCGGGTCCGATCCAGCGTCAGGATCTGGACAAGGTTTGTAAGACCTATGGGAAGGTGTTCCATTTCTGGCAGGTTGATAGGGGCGACATTCTACCTCTTGGGGTACCACAGGTTATGATGGCACTGACTAGGGATGGTCAGCTGTATGAAGATCTCGCTCGAAATGTGGAGAAGAGGTTTGGGGTATCCTTTGAGAAGGAGAGGGAGCACCGAGCTTATATGACGGGGCCGAATCTCGGAGTGCATCCTTTAGCAAACGGAGGGGGGAAGGGGTTGAAGACGGAGTTGAGGGAGGTGGATGTTCGGCCTGTCGATTCTGTTCCGAGGGTCTTTGTTTGA

Protein Analysis

235

Amino Acids

26.46

Weight (kDa)

5.93

Isoelectric Point (pI)

34.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1264 PF06884 30 - 183 5.7e-59 Protein of unknown function (DUF1264)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05510 AT2G31985
fragaria_vesca FvH4_2g06270 FvH4_2g06270
malus_domestica MD05G1004300.v1.1
prunus_persica Prupe.8G007500_v2.0.a1
pyrus_communis pycom05g00380
rosa_chinensis RchiOBHm_Chr6g0256911
rosa_laevigata RLG00000014656
rosa_multiflora Rmu_sc0003076.1_g000030
rosa_roxburghii Rroxscaffold_7G00208290
rosa_rugosa Rorug05G0577000
rosa_samantha Rh6AG095700 Rh6BG086500 Rh6DG078500
rosa_wichuraiana Rw6G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 427
Acc65I GGTACC 1 cut(s) 469
AccB1I GGYRCC 1 cut(s) 469
AccII CGCG 2 cut(s) 140, 182
AciI CCGC 3 cut(s) 66, 72, 75
AclWI GGATC 2 cut(s) 370, 396
AfaI GTAC 2 cut(s) 260, 471
AfiI CCNNNNNNNGG 4 cut(s) 193, 268, 385, 602
AflIII ACRYGT 1 cut(s) 152
AgsI TTSAA 1 cut(s) 643
AjiI CACGTC 1 cut(s) 155
AluBI AGCT 5 cut(s) 167, 244, 310, 508, 581
AluI AGCT 5 cut(s) 167, 244, 310, 508, 581
Alw21I GWGCWC 1 cut(s) 576
Alw26I GTCTC 2 cut(s) 268, 271
AlwI GGATC 2 cut(s) 370, 396
AlwNI CAGNNNCTG 1 cut(s) 391
Ama87I CYCGRG 2 cut(s) 227, 254
AoxI GGCC 5 cut(s) 91, 231, 250, 593, 671
Asp700I GAANNNNTTC 1 cut(s) 422
Asp718I GGTACC 1 cut(s) 469
AspLEI GCGC 4 cut(s) 125, 142, 182, 184
AspS9I GGNCC 4 cut(s) 92, 106, 371, 593
AsuC2I CCSGG 3 cut(s) 27, 360, 369
AsuHPI GGTGA 2 cut(s) 41, 161
AvaI CYCGRG 2 cut(s) 227, 254
AvaII GGWCC 2 cut(s) 106, 371
BanI GGYRCC 1 cut(s) 469
BauI CACGAG 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 650
Bbv12I GWGCWC 1 cut(s) 576
BccI CCATC 3 cut(s) 86, 478, 494
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BciVI GTATCC 1 cut(s) 562
BcnI CCSGG 3 cut(s) 27, 360, 369
BcoDI GTCTC 2 cut(s) 268, 271
BfaI CTAG 1 cut(s) 495
BfuAI ACCTGC 1 cut(s) 427
BfuI GTATCC 1 cut(s) 562
BglII AGATCT 1 cut(s) 517
BisI GCNGC 1 cut(s) 75
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 3 cut(s) 27, 360, 369
Bme18I GGWCC 2 cut(s) 106, 371
BmeT110I CYCGRG 2 cut(s) 227, 254
BmgBI CACGTC 1 cut(s) 155
BmgT120I GGNCC 4 cut(s) 92, 106, 371, 593
BmiI GGNNCC 4 cut(s) 94, 372, 471, 594
BmrFI CCNGG 3 cut(s) 27, 360, 369
BmsI GCATC 2 cut(s) 226, 619
BpiI GAAGAC 1 cut(s) 650
Bpu1102I GCTNAGC 1 cut(s) 240
BpuMI CCSGG 3 cut(s) 27, 360, 369
BsaJI CCNNGG 3 cut(s) 25, 228, 689
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 268, 385, 602
BseDI CCNNGG 3 cut(s) 25, 228, 689
BseGI GGATG 4 cut(s) 78, 505, 610, 670
BseLI CCNNNNNNNGG 4 cut(s) 193, 268, 385, 602
BsePI GCGCGC 1 cut(s) 180
BseRI GAGGAG 1 cut(s) 209
Bsh1236I CGCG 2 cut(s) 140, 182
BshFI GGCC 5 cut(s) 93, 233, 252, 595, 673
BshNI GGYRCC 1 cut(s) 469
BsiHKAI GWGCWC 1 cut(s) 576
BsiHKCI CYCGRG 2 cut(s) 227, 254
BsiSI CCGG 4 cut(s) 27, 45, 360, 368
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 4 cut(s) 193, 268, 385, 602
BsmAI GTCTC 2 cut(s) 268, 271
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 5 cut(s) 93, 233, 252, 595, 673
BsoBI CYCGRG 2 cut(s) 227, 254
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 3 cut(s) 375, 388, 517
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 3 cut(s) 66, 72, 75
BspANI GGCC 5 cut(s) 93, 233, 252, 595, 673
BspFNI CGCG 2 cut(s) 140, 182
BspLI GGNNCC 4 cut(s) 94, 372, 471, 594
BspMI ACCTGC 1 cut(s) 427
BspPI GGATC 2 cut(s) 370, 396
BspT107I GGYRCC 1 cut(s) 469
BssECI CCNNGG 3 cut(s) 25, 228, 689
BssHII GCGCGC 1 cut(s) 180
BssMI GATC 3 cut(s) 375, 388, 517
BssSI CACGAG 1 cut(s) 323
Bst2BI CACGAG 1 cut(s) 323
Bst6I CTCTTC 2 cut(s) 284, 533
BstC8I GCNNGC 1 cut(s) 182
BstDEI CTNAG 1 cut(s) 240
BstF5I GGATG 4 cut(s) 78, 505, 610, 670
BstFNI CGCG 2 cut(s) 140, 182
BstHHI GCGC 4 cut(s) 125, 142, 182, 184
BstKTI GATC 3 cut(s) 378, 391, 520
BstMAI GTCTC 2 cut(s) 268, 271
BstMBI GATC 3 cut(s) 375, 388, 517
BstMWI GCNNNNNNNGC 2 cut(s) 74, 364
BstSCI CCNGG 3 cut(s) 25, 358, 367
BstUI CGCG 2 cut(s) 140, 182
BstV2I GAAGAC 1 cut(s) 650
BstX2I RGATCY 2 cut(s) 388, 517
BstYI RGATCY 2 cut(s) 388, 517
BsuI GTATCC 1 cut(s) 562
BsuRI GGCC 5 cut(s) 93, 233, 252, 595, 673
BtrI CACGTC 1 cut(s) 155
BtsCI GGATG 4 cut(s) 78, 505, 610, 670
BtsIMutI CAGTG 2 cut(s) 212, 488
BveI ACCTGC 1 cut(s) 427
Cac8I GCNNGC 1 cut(s) 182
CaiI CAGNNNCTG 1 cut(s) 391
CfoI GCGC 4 cut(s) 125, 142, 182, 184
Cfr13I GGNCC 4 cut(s) 92, 106, 371, 593
CseI GACGC 2 cut(s) 191, 371
Csp6I GTAC 2 cut(s) 259, 470
CviAII CATG 3 cut(s) 55, 148, 355
CviQI GTAC 2 cut(s) 259, 470
DdeI CTNAG 1 cut(s) 240
DpnI GATC 3 cut(s) 377, 390, 519
DpnII GATC 3 cut(s) 375, 388, 517
Eam1104I CTCTTC 2 cut(s) 284, 533
EarI CTCTTC 2 cut(s) 284, 533
Eco47I GGWCC 2 cut(s) 106, 371
Eco88I CYCGRG 2 cut(s) 227, 254
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 3 cut(s) 58, 151, 358
FaiI YATR 9 cut(s) 56, 149, 330, 356, 414, 482, 513, 585, 587
FaqI GGGAC 1 cut(s) 8
FatI CATG 3 cut(s) 54, 147, 354
Fnu4HI GCNGC 1 cut(s) 75
FokI GGATG 4 cut(s) 65, 512, 597, 677
Fsp4HI GCNGC 1 cut(s) 75
FspBI CTAG 1 cut(s) 495
GlaI GCGC 4 cut(s) 124, 141, 181, 183
GluI GCNGC 1 cut(s) 75
HaeIII GGCC 5 cut(s) 93, 233, 252, 595, 673
HapII CCGG 4 cut(s) 27, 45, 360, 368
HgaI GACGC 2 cut(s) 191, 371
HhaI GCGC 4 cut(s) 125, 142, 182, 184
Hin1II CATG 3 cut(s) 58, 151, 358
Hin6I GCGC 4 cut(s) 123, 140, 180, 182
HinP1I GCGC 4 cut(s) 123, 140, 180, 182
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 5 cut(s) 21, 276, 286, 598, 680
HpaII CCGG 4 cut(s) 27, 45, 360, 368
HphI GGTGA 2 cut(s) 41, 161
Hpy166II GTNNAC 2 cut(s) 100, 109
Hpy188I TCNGA 4 cut(s) 271, 375, 605, 690
Hpy188III TCNNGA 5 cut(s) 283, 323, 337, 386, 392
Hpy8I GTNNAC 2 cut(s) 100, 109
HpyAV CCTTC 4 cut(s) 136, 412, 556, 628
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 1 cut(s) 610
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 364
HpyF3I CTNAG 1 cut(s) 240
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 3 cut(s) 58, 151, 358
HspAI GCGC 4 cut(s) 123, 140, 180, 182
KpnI GGTACC 1 cut(s) 473
Kzo9I GATC 3 cut(s) 375, 388, 517
LmnI GCTCC 1 cut(s) 571
LweI GCATC 2 cut(s) 226, 619
MaeI CTAG 1 cut(s) 495
MaeII ACGT 1 cut(s) 154
MalI GATC 3 cut(s) 377, 390, 519
MboI GATC 3 cut(s) 375, 388, 517
MboII GAAGA 5 cut(s) 271, 316, 527, 550, 655
MflI RGATCY 2 cut(s) 388, 517
MhlI GDGCHC 1 cut(s) 576
MlyI GAGTC 3 cut(s) 30, 270, 280
MroXI GAANNNNTTC 1 cut(s) 422
MslI CAYNNNNRTG 2 cut(s) 189, 327
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 4 cut(s) 27, 45, 360, 368
MspR9I CCNGG 3 cut(s) 27, 360, 369
MvnI CGCG 2 cut(s) 140, 182
MwoI GCNNNNNNNGC 2 cut(s) 74, 364
NciI CCSGG 3 cut(s) 27, 360, 369
NdeII GATC 3 cut(s) 375, 388, 517
NlaIII CATG 3 cut(s) 58, 151, 358
NlaIV GGNNCC 4 cut(s) 94, 372, 471, 594
PaeR7I CTCGAG 1 cut(s) 254
PauI GCGCGC 1 cut(s) 180
PcsI WCGNNNNNNNCGW 2 cut(s) 225, 261
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 2 cut(s) 598, 680
PkrI GCNGC 1 cut(s) 76
PleI GAGTC 3 cut(s) 29, 270, 280
PpsI GAGTC 3 cut(s) 29, 270, 280
PspN4I GGNNCC 4 cut(s) 94, 372, 471, 594
PspPI GGNCC 4 cut(s) 92, 106, 371, 593
PspXI VCTCGAGB 1 cut(s) 254
PstNI CAGNNNCTG 1 cut(s) 391
PsuI RGATCY 2 cut(s) 388, 517
PteI GCGCGC 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 508
RsaI GTAC 2 cut(s) 260, 471
RsaNI GTAC 2 cut(s) 259, 470
RseI CAYNNNNRTG 2 cut(s) 189, 327
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 3 cut(s) 375, 388, 517
Sau96I GGNCC 4 cut(s) 92, 106, 371, 593
SchI GAGTC 3 cut(s) 30, 270, 280
ScrFI CCNGG 3 cut(s) 27, 360, 369
SduI GDGCHC 1 cut(s) 576
SfaNI GCATC 2 cut(s) 226, 619
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 2 cut(s) 106, 371
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 2 cut(s) 189, 327
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SsiI CCGC 3 cut(s) 66, 72, 75
SspMI CTAG 1 cut(s) 495
StyD4I CCNGG 3 cut(s) 25, 358, 367
TaiI ACGT 1 cut(s) 157
TaqI TCGA 5 cut(s) 60, 162, 255, 526, 678
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 2 cut(s) 598, 680
TscAI CASTG 2 cut(s) 212, 495
TspDTI ATGAA 2 cut(s) 343, 528
TspGWI ACGGA 2 cut(s) 639, 662
TspRI CASTG 2 cut(s) 212, 495
VpaK11BI GGWCC 2 cut(s) 106, 371
XhoI CTCGAG 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 422
XspI CTAG 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.