RLG00000014656

Protein of unknown function (DUF1264)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0015191
Physical Location & Seq
Forward (+)
55853996 .. 55854839
844 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014656

Sequence Viewer

Length: 705 bp
ATGGCGACTAGTCACGCAGATGTCCCCGGCGAGCCAACCCAGACCGGAACAGCCATCCTCGAAACCGCTACCGCCGCCATCCAAGGCTTCGGCCCCGTCAACAAGATCCACCAGCACCTCTGCGCCTTCCACTTCTACGGCCACGACATGACACGTCAGGTCGAAGCTCACCATTTCTGTGGGCACCAGAATGAGGAGGTGCGTCAATGCCTCATCTACGACAGCCCCGAGGCCGGTGCTAAGCTCATCGGCCTCGAGTACCTCGTCTCGGAGAGACTCTTCTTGACTCTCCCTGACGACGAGAAGAAGCTGTGGCACTCTCACGAGTATGAGGTGAAGAGTGGGGTTTTGTTCATGCCCGGGGTGCCGGGTCCGATCCAGCGTCAAGATCTGGACAAGGTTTGTAAGACCTATGGGAAGGTGTTTCATTTCTGGCAGATTGATAGGGGCGACAATCTACCTCTTGGGGTGCCACAGGTGATGTTGGCATTGACTAGGGATGGTCAGCTGTATGAGGATCTTGCTCGAAATGTGGAGAAGAGGTTTGGGATATCCTTTGAGAAGGAGAGGGAGAACCGAGCTTATATGACCGGGCCGACTCTCGGAGTGCATCCTTTGGCAAACGGAGGAGGAAAAGGGTTGAAGACAGAGCTGAGGGAGGTGGGCGTTCAGCCGGTCGATTCTGTTCCGAGGGTCTTTGTCTGA

Protein Analysis

235

Amino Acids

26.17

Weight (kDa)

6.05

Isoelectric Point (pI)

32.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1264 PF06884 30 - 196 6.3e-74 Protein of unknown function (DUF1264)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05510 AT2G31985
fragaria_vesca FvH4_2g06270 FvH4_2g06270
malus_domestica MD05G1004300.v1.1
prunus_persica Prupe.8G007500_v2.0.a1
pyrus_communis pycom05g00380
rosa_chinensis RchiOBHm_Chr6g0256911
rosa_laevigata RLG00000014656
rosa_multiflora Rmu_sc0003076.1_g000030
rosa_roxburghii Rroxscaffold_7G00208290
rosa_rugosa Rorug05G0577000
rosa_samantha Rh6AG095700 Rh6BG086500 Rh6DG078500
rosa_wichuraiana Rw6G008150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 183, 364, 469
AciI CCGC 3 cut(s) 66, 72, 75
AclWI GGATC 3 cut(s) 100, 370, 525
AcoI YGGCCR 1 cut(s) 139
AfaI GTAC 1 cut(s) 260
AfiI CCNNNNNNNGG 4 cut(s) 193, 233, 268, 602
AflIII ACRYGT 1 cut(s) 152
AgsI TTSAA 1 cut(s) 643
AhlI ACTAGT 1 cut(s) 8
AjiI CACGTC 1 cut(s) 155
AluBI AGCT 6 cut(s) 167, 244, 310, 508, 581, 652
AluI AGCT 6 cut(s) 167, 244, 310, 508, 581, 652
Alw26I GTCTC 2 cut(s) 268, 271
AlwI GGATC 3 cut(s) 100, 370, 525
Ama87I CYCGRG 3 cut(s) 227, 254, 359
AoxI GGCC 5 cut(s) 91, 139, 231, 250, 593
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 3 cut(s) 92, 371, 593
AsuC2I CCSGG 5 cut(s) 27, 360, 361, 369, 592
AsuHPI GGTGA 3 cut(s) 161, 346, 490
AvaI CYCGRG 3 cut(s) 227, 254, 359
AvaII GGWCC 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 186
BanI GGYRCC 3 cut(s) 183, 364, 469
BauI CACGAG 1 cut(s) 323
BbsI GAAGAC 1 cut(s) 650
BbvCI CCTCAGC 1 cut(s) 653
BccI CCATC 3 cut(s) 62, 86, 494
BceAI ACGGC 1 cut(s) 154
BcgI CGANNNNNNTGC 2 cut(s) 218, 252
BcnI CCSGG 5 cut(s) 27, 360, 361, 369, 592
BcoDI GTCTC 2 cut(s) 268, 271
BcuI ACTAGT 1 cut(s) 8
BfaI CTAG 2 cut(s) 9, 495
BglII AGATCT 1 cut(s) 388
BisI GCNGC 1 cut(s) 75
BlpI GCTNAGC 1 cut(s) 240
BlsI GCNGC 1 cut(s) 76
Bme1390I CCNGG 5 cut(s) 27, 360, 361, 369, 592
Bme18I GGWCC 1 cut(s) 371
BmeT110I CYCGRG 3 cut(s) 227, 254, 359
BmgBI CACGTC 1 cut(s) 155
BmgT120I GGNCC 3 cut(s) 92, 371, 593
BmiI GGNNCC 5 cut(s) 94, 185, 366, 372, 471
BmrFI CCNGG 5 cut(s) 27, 360, 361, 369, 592
BmsI GCATC 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 650
Bpu10I CCTNAGC 1 cut(s) 653
Bpu1102I GCTNAGC 1 cut(s) 240
BpuMI CCSGG 5 cut(s) 27, 360, 361, 369, 592
BsaJI CCNNGG 6 cut(s) 25, 82, 228, 359, 360, 689
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 2 cut(s) 527, 557
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 233, 268, 602
Bse118I RCCGGY 2 cut(s) 233, 673
BseDI CCNNGG 6 cut(s) 25, 82, 228, 359, 360, 689
BseGI GGATG 4 cut(s) 54, 78, 505, 610
BseLI CCNNNNNNNGG 4 cut(s) 193, 233, 268, 602
BseMII CTCAG 1 cut(s) 644
BseRI GAGGAG 2 cut(s) 209, 642
BseSI GKGCMC 1 cut(s) 186
Bsh1285I CGRYCG 1 cut(s) 678
BshFI GGCC 5 cut(s) 93, 141, 233, 252, 595
BshNI GGYRCC 3 cut(s) 183, 364, 469
BsiEI CGRYCG 1 cut(s) 678
BsiHKCI CYCGRG 3 cut(s) 227, 254, 359
BsiSI CCGG 7 cut(s) 27, 45, 234, 360, 368, 591, 674
BslFI GGGAC 1 cut(s) 8
BslI CCNNNNNNNGG 4 cut(s) 193, 233, 268, 602
BsmAI GTCTC 2 cut(s) 268, 271
BsmBI CGTCTC 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 8
BsnI GGCC 5 cut(s) 93, 141, 233, 252, 595
BsoBI CYCGRG 3 cut(s) 227, 254, 359
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 4 cut(s) 105, 375, 388, 517
Bsp1720I GCTNAGC 1 cut(s) 240
BspACI CCGC 3 cut(s) 66, 72, 75
BspANI GGCC 5 cut(s) 93, 141, 233, 252, 595
BspCNI CTCAG 1 cut(s) 645
BspLI GGNNCC 5 cut(s) 94, 185, 366, 372, 471
BspPI GGATC 3 cut(s) 100, 370, 525
BspT107I GGYRCC 3 cut(s) 183, 364, 469
BsrFI RCCGGY 2 cut(s) 233, 673
BssAI RCCGGY 2 cut(s) 233, 673
BssECI CCNNGG 6 cut(s) 25, 82, 228, 359, 360, 689
BssMI GATC 4 cut(s) 105, 375, 388, 517
BssSI CACGAG 1 cut(s) 323
BssT1I CCWWGG 1 cut(s) 82
Bst2BI CACGAG 1 cut(s) 323
Bst6I CTCTTC 3 cut(s) 284, 332, 533
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 2 cut(s) 240, 653
BstF5I GGATG 4 cut(s) 54, 78, 505, 610
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 4 cut(s) 108, 378, 391, 520
BstMAI GTCTC 2 cut(s) 268, 271
BstMBI GATC 4 cut(s) 105, 375, 388, 517
BstMCI CGRYCG 1 cut(s) 678
BstMWI GCNNNNNNNGC 2 cut(s) 74, 364
BstSCI CCNGG 5 cut(s) 25, 358, 359, 367, 590
BstSLI GKGCMC 1 cut(s) 186
BstV2I GAAGAC 1 cut(s) 650
BstX2I RGATCY 3 cut(s) 105, 388, 517
BstXI CCANNNNNNTGG 1 cut(s) 179
BstYI RGATCY 3 cut(s) 105, 388, 517
BsuRI GGCC 5 cut(s) 93, 141, 233, 252, 595
BtrI CACGTC 1 cut(s) 155
BtsCI GGATG 4 cut(s) 54, 78, 505, 610
Cac8I GCNNGC 1 cut(s) 32
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 2 cut(s) 233, 673
Cfr13I GGNCC 3 cut(s) 92, 371, 593
Cfr9I CCCGGG 1 cut(s) 359
CseI GACGC 2 cut(s) 191, 371
Csp6I GTAC 1 cut(s) 259
CviAII CATG 2 cut(s) 148, 355
CviQI GTAC 1 cut(s) 259
DdeI CTNAG 2 cut(s) 240, 653
DpnI GATC 4 cut(s) 107, 377, 390, 519
DpnII GATC 4 cut(s) 105, 375, 388, 517
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 3 cut(s) 284, 332, 533
EarI CTCTTC 3 cut(s) 284, 332, 533
Eco130I CCWWGG 1 cut(s) 82
Eco32I GATATC 1 cut(s) 552
Eco47I GGWCC 1 cut(s) 371
Eco88I CYCGRG 3 cut(s) 227, 254, 359
EcoRV GATATC 1 cut(s) 552
EcoT14I CCWWGG 1 cut(s) 82
ErhI CCWWGG 1 cut(s) 82
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 2 cut(s) 151, 358
FaiI YATR 7 cut(s) 149, 330, 356, 414, 513, 585, 587
FaqI GGGAC 1 cut(s) 8
FatI CATG 2 cut(s) 147, 354
Fnu4HI GCNGC 1 cut(s) 75
FokI GGATG 4 cut(s) 41, 65, 512, 597
Fsp4HI GCNGC 1 cut(s) 75
FspBI CTAG 2 cut(s) 9, 495
GlaI GCGC 1 cut(s) 124
GluI GCNGC 1 cut(s) 75
HaeIII GGCC 5 cut(s) 93, 141, 233, 252, 595
HapII CCGG 7 cut(s) 27, 45, 234, 360, 368, 591, 674
HgaI GACGC 2 cut(s) 191, 371
HhaI GCGC 1 cut(s) 125
Hin1II CATG 2 cut(s) 151, 358
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 4 cut(s) 276, 286, 598, 680
HpaII CCGG 7 cut(s) 27, 45, 234, 360, 368, 591, 674
HphI GGTGA 3 cut(s) 161, 346, 490
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 5 cut(s) 271, 375, 605, 690, 704
Hpy188III TCNNGA 4 cut(s) 283, 323, 386, 392
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 3 cut(s) 136, 412, 556
HpyCH4IV ACGT 1 cut(s) 154
HpyCH4V TGCA 1 cut(s) 610
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 364
HpyF3I CTNAG 2 cut(s) 240, 653
HpySE526I ACGT 1 cut(s) 154
Hsp92II CATG 2 cut(s) 151, 358
HspAI GCGC 1 cut(s) 123
Kzo9I GATC 4 cut(s) 105, 375, 388, 517
LweI GCATC 1 cut(s) 619
MaeI CTAG 2 cut(s) 9, 495
MaeII ACGT 1 cut(s) 154
MaeIII GTNAC 1 cut(s) 11
MalI GATC 4 cut(s) 107, 377, 390, 519
MboI GATC 4 cut(s) 105, 375, 388, 517
MboII GAAGA 5 cut(s) 271, 316, 349, 550, 655
MflI RGATCY 3 cut(s) 105, 388, 517
MhlI GDGCHC 1 cut(s) 186
MlyI GAGTC 3 cut(s) 270, 280, 592
MslI CAYNNNNRTG 4 cut(s) 18, 177, 189, 327
MspA1I CMGCKG 1 cut(s) 508
MspI CCGG 7 cut(s) 27, 45, 234, 360, 368, 591, 674
MspR9I CCNGG 5 cut(s) 27, 360, 361, 369, 592
MwoI GCNNNNNNNGC 2 cut(s) 74, 364
NciI CCSGG 5 cut(s) 27, 360, 361, 369, 592
NdeII GATC 4 cut(s) 105, 375, 388, 517
NlaIII CATG 2 cut(s) 151, 358
NlaIV GGNNCC 5 cut(s) 94, 185, 366, 372, 471
NmuCI GTSAC 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 254
PcsI WCGNNNNNNNCGW 2 cut(s) 225, 261
PfeI GAWTC 1 cut(s) 680
PkrI GCNGC 1 cut(s) 76
PleI GAGTC 3 cut(s) 270, 280, 592
PpsI GAGTC 3 cut(s) 270, 280, 592
PspN4I GGNNCC 5 cut(s) 94, 185, 366, 372, 471
PspPI GGNCC 3 cut(s) 92, 371, 593
PspXI VCTCGAGB 1 cut(s) 254
PsuI RGATCY 3 cut(s) 105, 388, 517
PvuII CAGCTG 1 cut(s) 508
RsaI GTAC 1 cut(s) 260
RsaNI GTAC 1 cut(s) 259
RseI CAYNNNNRTG 4 cut(s) 18, 177, 189, 327
SatI GCNGC 1 cut(s) 75
Sau3AI GATC 4 cut(s) 105, 375, 388, 517
Sau96I GGNCC 3 cut(s) 92, 371, 593
SchI GAGTC 3 cut(s) 270, 280, 592
ScrFI CCNGG 5 cut(s) 27, 360, 361, 369, 592
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 1 cut(s) 619
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 1 cut(s) 371
SlaI CTCGAG 1 cut(s) 254
SmaI CCCGGG 1 cut(s) 361
SmiMI CAYNNNNRTG 4 cut(s) 18, 177, 189, 327
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
SpeI ACTAGT 1 cut(s) 8
SsiI CCGC 3 cut(s) 66, 72, 75
SspMI CTAG 2 cut(s) 9, 495
StyD4I CCNGG 5 cut(s) 25, 358, 359, 367, 590
StyI CCWWGG 1 cut(s) 82
TaiI ACGT 1 cut(s) 157
TaqI TCGA 5 cut(s) 60, 162, 255, 526, 678
TauI GCSGC 1 cut(s) 77
TfiI GAWTC 1 cut(s) 680
TseFI GTSAC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 2 cut(s) 343, 416
TspGWI ACGGA 1 cut(s) 639
TspMI CCCGGG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 371
XhoI CTCGAG 1 cut(s) 254
XmaI CCCGGG 1 cut(s) 359
XspI CTAG 2 cut(s) 9, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.