AT2G32150

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0010926
Physical Location & Seq
Forward (+)
13658888 .. 13660948
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32150.1

Sequence Viewer

Length: 792 bp
ATGGATTTCTCTCCGATCAACTGCCTCATTTTCGATTTGGATGACACATTATACCCTCTAAAAACTGGAATAGCGCCAGCTGTCAAGAAGAATATTGACGATTTTCTAGTGGAGAAATTTGGATTCTCAGAAAGTAAAGCTTCAAGCTTAAGAGTTGAACTCTTCAAGACTTATGGAAGTACTCTCGCTGGTCTTAGGGCGTTAGGTCATGATGTTCATCCTGATGAATATCACAGTTTCGTGCACGGAAGGTTACCGTATGGGTCGATCGAACCAAACAACAAGCTACGTAACCTTCTCAACAAAATCAAACAGAGAAAAATTATATTTACGAATTCGGACAAGAATCATGCGGTGAAGGTCCTCAAGAAACTTGGTTTAGAAGATTGTTTCGAAGAGATGATTTGTTTTGAGACAATGAACCCAAATTTATTCGGGTCAACAACCCGACCCGATGAATACCCGGTTGTTCTCAAGCCTTCGTTGACCGCAATGGATATTTGCATTCGTGTAGCCAACGTTGATCCTCGCCGAACAGTATTTTTGGATGATAATATCCACAATATTACCGCGGGTAAATCTGTCGGGCTTCGCACAATCCTGGTGGGAAGAGCAGAAAAGACGAAAGATGCGGATTATGCGGTGGAGACGGTTACTGAGATAGCTACGGCGGTGCCAGAGATTTGGGCTACGGCGACGGCGACAGGCGGATTTGACGTTGGTGGTGAGAGGATCAGACGGAGCAAGAGTGAGCTGGAGGGTATGGCTTCAATAGCAGCCGTCGGTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase Hydrolase_like

Protein Analysis

263

Amino Acids

29.04

Weight (kDa)

6.32

Isoelectric Point (pI)

38.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 6 - 196 7.1e-13 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 10 - 202 1.3e-08 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32150 AT2G32150 AT2G32150
fragaria_vesca FvH4_4g15270 FvH4_4g15270
malus_domestica MD13G1191400.v1.1 MD16G1191500.v1.1
prunus_persica Prupe.1G011600_v2.0.a1
pyrus_communis pycom13g16420 pycom16g16110
rosa_chinensis RchiOBHm_Chr4g0416551
rosa_laevigata RLG00000007974
rosa_multiflora Rmu_sc0003722.1_g000037
rosa_roxburghii Rroxscaffold_5G00360440
rosa_rugosa Rorug04G0142800
rosa_samantha Rh4AG205300 Rh4BG202900 Rh4CG216200 Rh4DG202400
rosa_wichuraiana Rw4G017380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 673
AccII CGCG 1 cut(s) 572
AciI CCGC 8 cut(s) 353, 489, 570, 572, 632, 641, 671, 708
AclI AACGTT 1 cut(s) 519
AclWI GGATC 2 cut(s) 518, 740
AcsI RAATTY 3 cut(s) 116, 334, 427
AfaI GTAC 1 cut(s) 181
AflII CTTAAG 1 cut(s) 148
AgsI TTSAA 4 cut(s) 144, 158, 166, 771
AjnI CCWGG 1 cut(s) 600
AluBI AGCT 6 cut(s) 80, 140, 147, 286, 665, 754
AluI AGCT 6 cut(s) 80, 140, 147, 286, 665, 754
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 2 cut(s) 407, 641
Alw44I GTGCAC 1 cut(s) 242
AlwI GGATC 2 cut(s) 518, 740
ApaLI GTGCAC 1 cut(s) 242
ApeKI GCWGC 1 cut(s) 776
ApoI RAATTY 3 cut(s) 116, 334, 427
AspLEI GCGC 1 cut(s) 76
AspS9I GGNCC 1 cut(s) 361
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 2 cut(s) 367, 737
AsuII TTCGAA 1 cut(s) 393
AvaII GGWCC 1 cut(s) 361
BaeGI GKGCMC 1 cut(s) 246
BanI GGYRCC 1 cut(s) 673
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 788
BceAI ACGGC 4 cut(s) 684, 708, 714, 764
BciT130I CCWGG 1 cut(s) 602
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 2 cut(s) 407, 641
BfaI CTAG 1 cut(s) 107
BfoI RGCGCY 1 cut(s) 77
BfrI CTTAAG 1 cut(s) 148
BisI GCNGC 1 cut(s) 777
BlsI GCNGC 1 cut(s) 778
BmcAI AGTACT 1 cut(s) 181
Bme1390I CCNGG 2 cut(s) 464, 602
Bme18I GGWCC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 675
BmrFI CCNGG 2 cut(s) 464, 602
BmsI GCATC 1 cut(s) 619
BplI GAGNNNNNCTC 2 cut(s) 144, 176
BpmI CTGGAG 1 cut(s) 776
Bpu14I TTCGAA 1 cut(s) 393
BpuEI CTTGAG 2 cut(s) 350, 458
BpuMI CCSGG 1 cut(s) 464
BsaAI YACGTR 1 cut(s) 290
BsaBI GATNNNNATC 2 cut(s) 216, 228
BsaJI CCNNGG 1 cut(s) 570
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bse1I ACTGG 1 cut(s) 70
Bse3DI GCAATG 1 cut(s) 498
Bse8I GATNNNNATC 2 cut(s) 216, 228
BseBI CCWGG 1 cut(s) 602
BseDI CCNNGG 1 cut(s) 570
BseGI GGATG 3 cut(s) 46, 217, 553
BseJI GATNNNNATC 2 cut(s) 216, 228
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 2 cut(s) 141, 648
BseNI ACTGG 1 cut(s) 70
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 1 cut(s) 788
Bsh1236I CGCG 1 cut(s) 572
Bsh1285I CGRYCG 1 cut(s) 270
BshNI GGYRCC 1 cut(s) 673
BsiEI CGRYCG 1 cut(s) 270
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 1 cut(s) 464
BsmAI GTCTC 2 cut(s) 407, 641
BsmBI CGTCTC 1 cut(s) 641
BsmI GAATGC 1 cut(s) 504
Bsp119I TTCGAA 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 246
Bsp143I GATC 4 cut(s) 15, 267, 523, 732
BspACI CCGC 8 cut(s) 353, 489, 570, 572, 632, 641, 671, 708
BspCNI CTCAG 2 cut(s) 140, 649
BspFNI CGCG 1 cut(s) 572
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 1 cut(s) 675
BspPI GGATC 2 cut(s) 518, 740
BspQI GCTCTTC 1 cut(s) 604
BspT104I TTCGAA 1 cut(s) 393
BspT107I GGYRCC 1 cut(s) 673
BspTI CTTAAG 1 cut(s) 148
BsrDI GCAATG 1 cut(s) 498
BsrI ACTGG 1 cut(s) 70
BssECI CCNNGG 1 cut(s) 570
BssMI GATC 4 cut(s) 15, 267, 523, 732
Bst2UI CCWGG 1 cut(s) 602
Bst4CI ACNGT 4 cut(s) 236, 258, 538, 652
Bst6I CTCTTC 3 cut(s) 167, 390, 604
BstAFI CTTAAG 1 cut(s) 148
BstBAI YACGTR 1 cut(s) 290
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 78
BstDEI CTNAG 3 cut(s) 127, 194, 657
BstDSI CCRYGG 1 cut(s) 570
BstEII GGTNACC 1 cut(s) 252
BstF5I GGATG 3 cut(s) 46, 217, 553
BstFNI CGCG 1 cut(s) 572
BstH2I RGCGCY 1 cut(s) 77
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 4 cut(s) 18, 270, 526, 735
BstMAI GTCTC 2 cut(s) 407, 641
BstMBI GATC 4 cut(s) 15, 267, 523, 732
BstMCI CGRYCG 1 cut(s) 270
BstMWI GCNNNNNNNGC 3 cut(s) 638, 773, 785
BstNI CCWGG 1 cut(s) 602
BstPI GGTNACC 1 cut(s) 252
BstSCI CCNGG 2 cut(s) 462, 600
BstSLI GKGCMC 1 cut(s) 246
BstSNI TACGTA 1 cut(s) 290
BstUI CGCG 1 cut(s) 572
BstV1I GCAGC 1 cut(s) 788
BstXI CCANNNNNNTGG 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 570
BtsCI GGATG 3 cut(s) 46, 217, 553
Cac8I GCNNGC 1 cut(s) 78
CciI TCATGA 1 cut(s) 208
CfoI GCGC 1 cut(s) 76
Cfr13I GGNCC 1 cut(s) 361
Cfr42I CCGCGG 1 cut(s) 573
Csp6I GTAC 1 cut(s) 180
CspCI CAANNNNNGTGG 2 cut(s) 585, 620
CviAII CATG 2 cut(s) 209, 350
CviQI GTAC 1 cut(s) 180
DdeI CTNAG 3 cut(s) 127, 194, 657
DpnI GATC 4 cut(s) 17, 269, 525, 734
DpnII GATC 4 cut(s) 15, 267, 523, 732
Eam1104I CTCTTC 3 cut(s) 167, 390, 604
EarI CTCTTC 3 cut(s) 167, 390, 604
EciI GGCGGA 1 cut(s) 723
Eco105I TACGTA 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 361
Eco91I GGTNACC 1 cut(s) 252
EcoO109I RGGNCCY 1 cut(s) 361
EcoO65I GGTNACC 1 cut(s) 252
EcoRI GAATTC 1 cut(s) 334
EcoRII CCWGG 1 cut(s) 600
Esp3I CGTCTC 1 cut(s) 641
FaeI CATG 2 cut(s) 212, 353
FaiI YATR 8 cut(s) 52, 174, 210, 261, 326, 351, 639, 764
FalI AAGNNNNNCTT 2 cut(s) 124, 156
FatI CATG 2 cut(s) 208, 349
FauI CCCGC 1 cut(s) 565
Fnu4HI GCNGC 1 cut(s) 777
FokI GGATG 3 cut(s) 53, 204, 560
Fsp4HI GCNGC 1 cut(s) 777
FspBI CTAG 1 cut(s) 107
GlaI GCGC 1 cut(s) 75
GluI GCNGC 1 cut(s) 777
GsuI CTGGAG 1 cut(s) 776
HaeII RGCGCY 1 cut(s) 77
HapII CCGG 1 cut(s) 464
HhaI GCGC 1 cut(s) 76
Hin1II CATG 2 cut(s) 212, 353
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HincII GTYRAC 2 cut(s) 441, 486
HindII GTYRAC 2 cut(s) 441, 486
HindIII AAGCTT 2 cut(s) 138, 145
HinfI GANTC 2 cut(s) 123, 346
HpaII CCGG 1 cut(s) 464
HphI GGTGA 2 cut(s) 367, 737
Hpy166II GTNNAC 3 cut(s) 244, 441, 486
Hpy188I TCNGA 4 cut(s) 15, 130, 340, 737
Hpy188III TCNNGA 5 cut(s) 85, 166, 209, 221, 367
Hpy8I GTNNAC 3 cut(s) 244, 441, 486
Hpy99I CGWCG 2 cut(s) 700, 785
HpyAV CCTTC 4 cut(s) 243, 305, 352, 489
HpyCH4III ACNGT 4 cut(s) 236, 258, 538, 652
HpyCH4IV ACGT 3 cut(s) 289, 519, 717
HpyCH4V TGCA 2 cut(s) 244, 504
HpyF10VI GCNNNNNNNGC 3 cut(s) 638, 773, 785
HpyF3I CTNAG 3 cut(s) 127, 194, 657
HpySE526I ACGT 3 cut(s) 289, 519, 717
Hsp92II CATG 2 cut(s) 212, 353
HspAI GCGC 1 cut(s) 74
KspI CCGCGG 1 cut(s) 573
Kzo9I GATC 4 cut(s) 15, 267, 523, 732
LguI GCTCTTC 1 cut(s) 604
LmnI GCTCC 1 cut(s) 741
Lsp1109I GCAGC 1 cut(s) 788
LweI GCATC 1 cut(s) 619
MaeI CTAG 1 cut(s) 107
MaeII ACGT 3 cut(s) 289, 519, 717
MaeIII GTNAC 3 cut(s) 252, 290, 652
MalI GATC 4 cut(s) 17, 269, 525, 734
MboI GATC 4 cut(s) 15, 267, 523, 732
MboII GAAGA 5 cut(s) 100, 154, 395, 407, 621
MhlI GDGCHC 1 cut(s) 246
MluCI AATT 4 cut(s) 116, 321, 334, 427
MnlI CCTC 6 cut(s) 35, 66, 374, 537, 723, 751
MseI TTAA 1 cut(s) 149
MslI CAYNNNNRTG 1 cut(s) 222
MspA1I CMGCKG 2 cut(s) 80, 572
MspCI CTTAAG 1 cut(s) 148
MspI CCGG 1 cut(s) 464
MspR9I CCNGG 2 cut(s) 464, 602
Mva1269I GAATGC 1 cut(s) 504
MvaI CCWGG 1 cut(s) 602
MvnI CGCG 1 cut(s) 572
MwoI GCNNNNNNNGC 3 cut(s) 638, 773, 785
NciI CCSGG 1 cut(s) 464
NdeII GATC 4 cut(s) 15, 267, 523, 732
NlaIII CATG 2 cut(s) 212, 353
NlaIV GGNNCC 1 cut(s) 675
NspV TTCGAA 1 cut(s) 393
PagI TCATGA 1 cut(s) 208
PciSI GCTCTTC 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 698
PctI GAATGC 1 cut(s) 504
PfeI GAWTC 2 cut(s) 123, 346
PkrI GCNGC 1 cut(s) 778
Ple19I CGATCG 1 cut(s) 270
Ppu21I YACGTR 1 cut(s) 290
PpuMI RGGWCCY 1 cut(s) 361
Psp1406I AACGTT 1 cut(s) 519
Psp5II RGGWCCY 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 600
PspEI GGTNACC 1 cut(s) 252
PspGI CCWGG 1 cut(s) 600
PspN4I GGNNCC 1 cut(s) 675
PspPI GGNCC 1 cut(s) 361
PspPPI RGGWCCY 1 cut(s) 361
PvuI CGATCG 1 cut(s) 270
PvuII CAGCTG 1 cut(s) 80
RsaI GTAC 1 cut(s) 181
RsaNI GTAC 1 cut(s) 180
RseI CAYNNNNRTG 1 cut(s) 222
SacII CCGCGG 1 cut(s) 573
SapI GCTCTTC 1 cut(s) 604
SaqAI TTAA 1 cut(s) 149
SatI GCNGC 1 cut(s) 777
Sau3AI GATC 4 cut(s) 15, 267, 523, 732
Sau96I GGNCC 1 cut(s) 361
ScaI AGTACT 1 cut(s) 181
ScrFI CCNGG 2 cut(s) 464, 602
SduI GDGCHC 1 cut(s) 246
SfaNI GCATC 1 cut(s) 619
Sfr303I CCGCGG 1 cut(s) 573
SfuI TTCGAA 1 cut(s) 393
SgrBI CCGCGG 1 cut(s) 573
SinI GGWCC 1 cut(s) 361
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 3 cut(s) 148, 365, 473
SmoI CTYRAG 3 cut(s) 148, 365, 473
SnaBI TACGTA 1 cut(s) 290
Sse9I AATT 4 cut(s) 116, 321, 334, 427
SsiI CCGC 8 cut(s) 353, 489, 570, 572, 632, 641, 671, 708
SspI AATATT 2 cut(s) 94, 565
SspMI CTAG 1 cut(s) 107
StyD4I CCNGG 2 cut(s) 462, 600
TaaI ACNGT 4 cut(s) 236, 258, 538, 652
TaiI ACGT 3 cut(s) 292, 522, 720
TaqI TCGA 4 cut(s) 33, 266, 270, 393
TasI AATT 4 cut(s) 116, 321, 334, 427
TatI WGTACW 1 cut(s) 179
TfiI GAWTC 2 cut(s) 123, 346
Tru1I TTAA 1 cut(s) 149
Tru9I TTAA 1 cut(s) 149
TseI GCWGC 1 cut(s) 776
TspDTI ATGAA 4 cut(s) 206, 240, 434, 471
TspGWI ACGGA 2 cut(s) 261, 754
Vha464I CTTAAG 1 cut(s) 148
VneI GTGCAC 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 361
XapI RAATTY 3 cut(s) 116, 334, 427
XspI CTAG 1 cut(s) 107
ZrmI AGTACT 1 cut(s) 181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.