Rh4CG216200

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010926
Physical Location & Seq
Forward (+)
44745720 .. 44748164
2445 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG216200.1

Sequence Viewer

Length: 807 bp
ATGGATTCCTGCAATTCCTCTCTTCGCGGTTCCTCCTCTCCTTTCGATTGCCTCATCTTCGATTTAGATGACACTTTGTACTCTTCAAGCATTGGAATCGGTGAAGCTCTCAAGAAGAACATCGATGATTTTCTTGTTGAAAAATGCGGGTTCGCCGAGAGCAAAGCCTCGAGTCTCCGAGTTGAGCTTTTCAAAAAGTACGGCAGCTCCCTCGCCGGATTACGGGCATTAGGCTATGACATCGATGCTGACGATTACCACAGTTTCGTCCACGGAAGGTTGCCTTACGATTTAATCAAACCCGATCCTCAGCTACGAAACCTCATTCGCAGCATTTCGCAGAGAAAAATCATTTTTACGAATTCGGACAGGAACCATGCGGTGACGGTGTTGGATCGTCTGGGGTTGAAGGGCTGCTTCGAACAGATCATATGTTTTGAAACGATGAACCCGAAGCTAGCCGAGTCGACTCGGCCGGACGAGTTCCCGGTGGTTCTGAAGCCGTCCATGGAGGCCATGAGGATTGCGATCGAAGCTGCTGAGGTCGATCCTCGTCGGACGCTTTTTCTCGATGACAATGTCCGAAACGTCGCTGCCGGGAGAGCCGTGGGTCTACGCACCGTTTTGGTTGGAAAAACCGTCAAGAGCAAGGAAGCAGATTATGTGTCAGAGAACGTGAAGAGCATGGCACAATCAATCTCTGAAGTGTGGGTAGGCGGATCGGACGGTAGTAATCAACGAGTCAATGGTACCAGAAGCGACATCGAGGTGGATTCCATTCGGACAACCACCGCAGTTGTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase Hydrolase_like

Protein Analysis

268

Amino Acids

29.51

Weight (kDa)

5.54

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 16 - 204 1.5e-14 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 19 - 210 1.6e-08 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 167 - 219 2.4e-06 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32150 AT2G32150 AT2G32150
fragaria_vesca FvH4_4g15270 FvH4_4g15270
malus_domestica MD13G1191400.v1.1 MD16G1191500.v1.1
prunus_persica Prupe.1G011600_v2.0.a1
pyrus_communis pycom13g16420 pycom16g16110
rosa_chinensis RchiOBHm_Chr4g0416551
rosa_laevigata RLG00000007974
rosa_multiflora Rmu_sc0003722.1_g000037
rosa_roxburghii Rroxscaffold_5G00360440
rosa_rugosa Rorug04G0142800
rosa_samantha Rh4AG205300 Rh4BG202900 Rh4CG216200 Rh4DG202400
rosa_wichuraiana Rw4G017380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 749
AccB1I GGYRCC 1 cut(s) 749
AccI GTMKAC 2 cut(s) 467, 613
AccII CGCG 1 cut(s) 27
AciI CCGC 5 cut(s) 27, 147, 380, 717, 792
AclWI GGATC 4 cut(s) 299, 402, 542, 727
AcoI YGGCCR 1 cut(s) 473
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 2 cut(s) 518, 723
AfaI GTAC 3 cut(s) 80, 200, 751
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 5 cut(s) 87, 140, 193, 409, 440
AluBI AGCT 7 cut(s) 107, 187, 207, 313, 457, 536, 803
AluI AGCT 7 cut(s) 107, 187, 207, 313, 457, 536, 803
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 4 cut(s) 299, 402, 542, 727
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 2 cut(s) 473, 513
ApeKI GCWGC 5 cut(s) 204, 330, 414, 536, 593
ApoI RAATTY 1 cut(s) 361
Asp718I GGTACC 1 cut(s) 749
AsuC2I CCSGG 2 cut(s) 488, 598
AsuHPI GGTGA 2 cut(s) 113, 394
AsuII TTCGAA 1 cut(s) 420
AsuNHI GCTAGC 1 cut(s) 457
AvaI CYCGRG 1 cut(s) 169
BanI GGYRCC 1 cut(s) 749
BbvCI CCTCAGC 2 cut(s) 309, 540
BbvI GCAGC 5 cut(s) 216, 342, 401, 523, 580
BceAI ACGGC 3 cut(s) 217, 487, 590
BcgI CGANNNNNNTGC 4 cut(s) 79, 113, 193, 227
BcnI CCSGG 2 cut(s) 488, 598
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 1 cut(s) 458
BisI GCNGC 5 cut(s) 205, 331, 415, 537, 594
BlsI GCNGC 5 cut(s) 206, 332, 416, 538, 595
Bme1390I CCNGG 2 cut(s) 488, 598
BmeT110I CYCGRG 1 cut(s) 169
BmiI GGNNCC 3 cut(s) 31, 374, 751
BmrFI CCNGG 2 cut(s) 488, 598
BmsI GCATC 1 cut(s) 235
BmtI GCTAGC 1 cut(s) 461
Bpu10I CCTNAGC 2 cut(s) 309, 540
Bpu14I TTCGAA 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 2 cut(s) 488, 598
Bsa29I ATCGAT 2 cut(s) 123, 243
BsaBI GATNNNNATC 1 cut(s) 527
BsaJI CCNNGG 3 cut(s) 271, 507, 606
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 527
BseCI ATCGAT 2 cut(s) 123, 243
BseDI CCNNGG 3 cut(s) 271, 507, 606
BseJI GATNNNNATC 1 cut(s) 527
BseLI CCNNNNNNNGG 1 cut(s) 222
BseMII CTCAG 2 cut(s) 323, 531
BseRI GAGGAG 1 cut(s) 25
BseX3I CGGCCG 1 cut(s) 473
BseXI GCAGC 5 cut(s) 216, 342, 401, 523, 580
Bsh1236I CGCG 1 cut(s) 27
Bsh1285I CGRYCG 2 cut(s) 476, 531
BshFI GGCC 2 cut(s) 475, 515
BshNI GGYRCC 1 cut(s) 749
BshVI ATCGAT 2 cut(s) 123, 243
BsiEI CGRYCG 2 cut(s) 476, 531
BsiHKCI CYCGRG 1 cut(s) 169
BsiSI CCGG 4 cut(s) 216, 476, 488, 597
BslI CCNNNNNNNGG 1 cut(s) 222
BsmAI GTCTC 1 cut(s) 179
BsnI GGCC 2 cut(s) 475, 515
BsoBI CYCGRG 1 cut(s) 169
Bsp119I TTCGAA 1 cut(s) 420
Bsp143I GATC 6 cut(s) 304, 394, 426, 528, 547, 719
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 5 cut(s) 27, 147, 380, 717, 792
BspANI GGCC 2 cut(s) 475, 515
BspCNI CTCAG 2 cut(s) 322, 532
BspDI ATCGAT 2 cut(s) 123, 243
BspFNI CGCG 1 cut(s) 27
BspLI GGNNCC 3 cut(s) 31, 374, 751
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 4 cut(s) 299, 402, 542, 727
BspQI GCTCTTC 1 cut(s) 674
BspT104I TTCGAA 1 cut(s) 420
BspT107I GGYRCC 1 cut(s) 749
BssECI CCNNGG 3 cut(s) 271, 507, 606
BssMI GATC 6 cut(s) 304, 394, 426, 528, 547, 719
BssT1I CCWWGG 1 cut(s) 507
Bst4CI ACNGT 5 cut(s) 263, 388, 622, 640, 728
Bst6I CTCTTC 3 cut(s) 27, 88, 674
BstBI TTCGAA 1 cut(s) 420
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 2 cut(s) 309, 540
BstDSI CCRYGG 3 cut(s) 271, 507, 606
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 6 cut(s) 307, 397, 429, 531, 550, 722
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 6 cut(s) 304, 394, 426, 528, 547, 719
BstMCI CGRYCG 2 cut(s) 476, 531
BstMWI GCNNNNNNNGC 3 cut(s) 533, 602, 800
BstSCI CCNGG 2 cut(s) 486, 596
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 5 cut(s) 216, 342, 401, 523, 580
BstZI CGGCCG 1 cut(s) 473
Bsu15I ATCGAT 2 cut(s) 123, 243
BsuRI GGCC 2 cut(s) 475, 515
BsuTUI ATCGAT 2 cut(s) 123, 243
BtgI CCRYGG 3 cut(s) 271, 507, 606
Cac8I GCNNGC 1 cut(s) 459
ClaI ATCGAT 2 cut(s) 123, 243
CseI GACGC 1 cut(s) 568
Csp6I GTAC 3 cut(s) 79, 199, 750
CspCI CAANNNNNGTGG 1 cut(s) 778
CviAII CATG 4 cut(s) 377, 508, 517, 685
CviQI GTAC 3 cut(s) 79, 199, 750
DdeI CTNAG 2 cut(s) 309, 540
DpnI GATC 6 cut(s) 306, 396, 428, 530, 549, 721
DpnII GATC 6 cut(s) 304, 394, 426, 528, 547, 719
EaeI YGGCCR 1 cut(s) 473
EagI CGGCCG 1 cut(s) 473
Eam1104I CTCTTC 3 cut(s) 27, 88, 674
EarI CTCTTC 3 cut(s) 27, 88, 674
EciI GGCGGA 1 cut(s) 732
EclXI CGGCCG 1 cut(s) 473
Eco130I CCWWGG 1 cut(s) 507
Eco52I CGGCCG 1 cut(s) 473
Eco57I CTGAAG 2 cut(s) 518, 723
Eco88I CYCGRG 1 cut(s) 169
EcoRI GAATTC 1 cut(s) 361
EcoT14I CCWWGG 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 4 cut(s) 380, 511, 520, 688
FaiI YATR 8 cut(s) 237, 378, 431, 433, 509, 518, 663, 686
FalI AAGNNNNNCTT 4 cut(s) 268, 300, 401, 433
FatI CATG 4 cut(s) 376, 507, 516, 684
FauI CCCGC 1 cut(s) 140
FauNDI CATATG 1 cut(s) 431
FblI GTMKAC 2 cut(s) 467, 613
Fnu4HI GCNGC 5 cut(s) 205, 331, 415, 537, 594
Fsp4HI GCNGC 5 cut(s) 205, 331, 415, 537, 594
FspBI CTAG 1 cut(s) 458
GluI GCNGC 5 cut(s) 205, 331, 415, 537, 594
HaeIII GGCC 2 cut(s) 475, 515
HapII CCGG 4 cut(s) 216, 476, 488, 597
HgaI GACGC 1 cut(s) 568
Hin1II CATG 4 cut(s) 380, 511, 520, 688
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HinfI GANTC 7 cut(s) 5, 96, 172, 464, 469, 741, 773
HpaII CCGG 4 cut(s) 216, 476, 488, 597
HphI GGTGA 2 cut(s) 113, 394
Hpy166II GTNNAC 3 cut(s) 271, 468, 614
Hpy188I TCNGA 9 cut(s) 179, 367, 498, 558, 584, 670, 703, 724, 783
Hpy188III TCNNGA 3 cut(s) 112, 569, 643
Hpy8I GTNNAC 3 cut(s) 271, 468, 614
Hpy99I CGWCG 2 cut(s) 558, 593
HpyAV CCTTC 2 cut(s) 270, 403
HpyCH4III ACNGT 5 cut(s) 263, 388, 622, 640, 728
HpyCH4IV ACGT 2 cut(s) 588, 675
HpyCH4V TGCA 1 cut(s) 12
HpyF10VI GCNNNNNNNGC 3 cut(s) 533, 602, 800
HpyF3I CTNAG 2 cut(s) 309, 540
HpySE526I ACGT 2 cut(s) 588, 675
Hsp92II CATG 4 cut(s) 380, 511, 520, 688
KpnI GGTACC 1 cut(s) 753
Kzo9I GATC 6 cut(s) 304, 394, 426, 528, 547, 719
LguI GCTCTTC 1 cut(s) 674
LmnI GCTCC 1 cut(s) 212
LpnPI CCDG 8 cut(s) 22, 229, 355, 386, 489, 501, 610, 766
Lsp1109I GCAGC 5 cut(s) 216, 342, 401, 523, 580
LweI GCATC 1 cut(s) 235
MaeI CTAG 1 cut(s) 458
MaeII ACGT 2 cut(s) 588, 675
MaeIII GTNAC 1 cut(s) 382
MalI GATC 6 cut(s) 306, 396, 428, 530, 549, 721
MboI GATC 6 cut(s) 304, 394, 426, 528, 547, 719
MboII GAAGA 5 cut(s) 14, 49, 75, 127, 691
MluCI AATT 2 cut(s) 13, 361
MlyI GAGTC 4 cut(s) 181, 463, 473, 750
MmeI TCCRAC 3 cut(s) 372, 536, 610
MseI TTAA 2 cut(s) 293, 805
MslI CAYNNNNRTG 1 cut(s) 767
MspI CCGG 4 cut(s) 216, 476, 488, 597
MspR9I CCNGG 2 cut(s) 488, 598
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 3 cut(s) 533, 602, 800
NciI CCSGG 2 cut(s) 488, 598
NcoI CCATGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 431
NdeII GATC 6 cut(s) 304, 394, 426, 528, 547, 719
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 4 cut(s) 380, 511, 520, 688
NlaIV GGNNCC 3 cut(s) 31, 374, 751
NmeAIII GCCGAG 3 cut(s) 181, 451, 487
NmuCI GTSAC 1 cut(s) 382
NspV TTCGAA 1 cut(s) 420
PaeR7I CTCGAG 1 cut(s) 169
PciSI GCTCTTC 1 cut(s) 674
PcsI WCGNNNNNNNCGW 2 cut(s) 249, 449
PfeI GAWTC 3 cut(s) 5, 96, 773
PflFI GACNNNGTC 1 cut(s) 578
PkrI GCNGC 5 cut(s) 206, 332, 416, 538, 595
Ple19I CGATCG 1 cut(s) 531
PleI GAGTC 4 cut(s) 180, 463, 472, 749
PpsI GAGTC 4 cut(s) 180, 463, 472, 749
PspN4I GGNNCC 3 cut(s) 31, 374, 751
PspXI VCTCGAGB 1 cut(s) 169
PsyI GACNNNGTC 1 cut(s) 578
PvuI CGATCG 1 cut(s) 531
RsaI GTAC 3 cut(s) 80, 200, 751
RsaNI GTAC 3 cut(s) 79, 199, 750
RseI CAYNNNNRTG 1 cut(s) 767
SalI GTCGAC 1 cut(s) 466
SapI GCTCTTC 1 cut(s) 674
SaqAI TTAA 2 cut(s) 293, 805
SatI GCNGC 5 cut(s) 205, 331, 415, 537, 594
Sau3AI GATC 6 cut(s) 304, 394, 426, 528, 547, 719
SchI GAGTC 4 cut(s) 181, 463, 473, 750
ScrFI CCNGG 2 cut(s) 488, 598
SfaNI GCATC 1 cut(s) 235
Sfr274I CTCGAG 1 cut(s) 169
SfuI TTCGAA 1 cut(s) 420
SlaI CTCGAG 1 cut(s) 169
SmiMI CAYNNNNRTG 1 cut(s) 767
SmlI CTYRAG 2 cut(s) 110, 169
SmoI CTYRAG 2 cut(s) 110, 169
Sse9I AATT 2 cut(s) 13, 361
SsiI CCGC 5 cut(s) 27, 147, 380, 717, 792
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 2 cut(s) 486, 596
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 5 cut(s) 263, 388, 622, 640, 728
TaiI ACGT 2 cut(s) 591, 678
TasI AATT 2 cut(s) 13, 361
TatI WGTACW 1 cut(s) 78
TfiI GAWTC 3 cut(s) 5, 96, 773
Tru1I TTAA 2 cut(s) 293, 805
Tru9I TTAA 2 cut(s) 293, 805
TseFI GTSAC 1 cut(s) 382
TseI GCWGC 5 cut(s) 204, 330, 414, 536, 593
Tsp45I GTSAC 1 cut(s) 382
TspDTI ATGAA 1 cut(s) 461
TspGWI ACGGA 1 cut(s) 288
Tth111I GACNNNGTC 1 cut(s) 578
XapI RAATTY 1 cut(s) 361
XhoI CTCGAG 1 cut(s) 169
XmiI GTMKAC 2 cut(s) 467, 613
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.