Rorug04G0142800

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010926
Physical Location & Seq
Reverse (-)
23788690 .. 23789349
660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0142800.1

Sequence Viewer

Length: 660 bp
ATGTGGAACGATGCCTGGATACCCAAGTTACATGCTCATAAGCCTCTTGGTGCTTTAGTGAGTCAACATAATGTGAATATGGTGGATGAACTGATTACTCCTCCAAGTAATTGGAATGAGAGCAAGGTGCGTTCCATTTTTACTCCTGAGGATGCAGAAGCAATACTTGCTATTCCTTTGAGCTCTAGACAACCAGAAGACAGGTTAACATGGCATTTTGAGAGAAAAGGTATGTTCACTGTCAAATCAGCTTATCGTTTTGCCTTCTCAAAAAATGCTTTTACTCTTCCTCCTTCTGTTGTGGCTAATCAGCGAGTTTGGAAAAAAATTTGGAATGCTAAGATGCCTAGTTCTGCTAAGGTTACTGCATGGAAAATTTGTCATAATATCTTGCCTACTAGGGACCGATTAGCTTCTAGACATGTGGAGTTGGCGTCTCAGATTTGCGTTCTATGCAATGCTGATGCAGAGACAGTTATTCATATGTGTCATAATTGTACTTTTACAAGGGATGTGTTTAGTACCAATGTTAGCATCAAGGGTTGTTGTCTAGGGACTGATTCTGATGATTTGGATGCCTTGAGTTGGTTGACGTTTTGCTCTGAGAAACTGTCAAAGACTGCCTTTGCTCAGCTTTTATTTTTGATTTGGGGAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase Hydrolase_like

Protein Analysis

219

Amino Acids

24.82

Weight (kDa)

8.64

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RVT PF13966 79 - 172 2.8e-17 zinc-binding in reverse transcriptase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32150 AT2G32150 AT2G32150
fragaria_vesca FvH4_4g15270 FvH4_4g15270
malus_domestica MD13G1191400.v1.1 MD16G1191500.v1.1
prunus_persica Prupe.1G011600_v2.0.a1
pyrus_communis pycom13g16420 pycom16g16110
rosa_chinensis RchiOBHm_Chr4g0416551
rosa_laevigata RLG00000007974
rosa_multiflora Rmu_sc0003722.1_g000037
rosa_roxburghii Rroxscaffold_5G00360440
rosa_rugosa Rorug04G0142800
rosa_samantha Rh4AG205300 Rh4BG202900 Rh4CG216200 Rh4DG202400
rosa_wichuraiana Rw4G017380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 327, 375
AcyI GRCGYC 1 cut(s) 434
AfaI GTAC 2 cut(s) 499, 523
AfiI CCNNNNNNNGG 1 cut(s) 585
AflIII ACRYGT 1 cut(s) 421
AjnI CCWGG 1 cut(s) 14
AluBI AGCT 4 cut(s) 183, 251, 413, 634
AluI AGCT 4 cut(s) 183, 251, 413, 634
Alw21I GWGCWC 1 cut(s) 185
Alw26I GTCTC 2 cut(s) 441, 464
ApoI RAATTY 2 cut(s) 327, 375
AspS9I GGNCC 1 cut(s) 403
AvaII GGWCC 1 cut(s) 403
AxyI CCTNAGG 1 cut(s) 147
BaeI ACNNNNGTAYC 2 cut(s) 11, 44
BanII GRGCYC 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 16
BciVI GTATCC 1 cut(s) 12
BcoDI GTCTC 2 cut(s) 441, 464
BfaI CTAG 5 cut(s) 186, 348, 399, 417, 551
BfuI GTATCC 1 cut(s) 12
BlpI GCTNAGC 1 cut(s) 630
Bme1390I CCNGG 1 cut(s) 16
Bme18I GGWCC 1 cut(s) 403
BmgT120I GGNCC 1 cut(s) 403
BmiI GGNNCC 1 cut(s) 404
BmrFI CCNGG 1 cut(s) 16
BmsI GCATC 5 cut(s) 142, 333, 454, 543, 565
BpiI GAAGAC 1 cut(s) 204
Bpu10I CCTNAGC 1 cut(s) 357
Bpu1102I GCTNAGC 1 cut(s) 630
BpuEI CTTGAG 1 cut(s) 601
BsaHI GRCGYC 1 cut(s) 434
BsaXI ACNNNNNCTCC 4 cut(s) 274, 304, 419, 449
Bsc4I CCNNNNNNNGG 1 cut(s) 585
Bse21I CCTNAGG 1 cut(s) 147
Bse3DI GCAATG 1 cut(s) 463
BseBI CCWGG 1 cut(s) 16
BseGI GGATG 4 cut(s) 91, 157, 517, 580
BseLI CCNNNNNNNGG 1 cut(s) 585
BseMI GCAATG 1 cut(s) 463
BseMII CTCAG 4 cut(s) 138, 452, 594, 644
BseRI GAGGAG 1 cut(s) 90
BsiHKAI GWGCWC 1 cut(s) 185
BslFI GGGAC 2 cut(s) 416, 568
BslI CCNNNNNNNGG 1 cut(s) 585
BsmAI GTCTC 2 cut(s) 441, 464
BsmBI CGTCTC 1 cut(s) 441
BsmFI GGGAC 2 cut(s) 416, 568
BsmI GAATGC 1 cut(s) 340
Bsp1286I GDGCHC 1 cut(s) 185
Bsp1720I GCTNAGC 1 cut(s) 630
BspCNI CTCAG 4 cut(s) 139, 451, 595, 643
BspLI GGNNCC 1 cut(s) 404
BsrDI GCAATG 1 cut(s) 463
BssNI GRCGYC 1 cut(s) 434
Bst2UI CCWGG 1 cut(s) 16
Bst4CI ACNGT 3 cut(s) 241, 475, 612
Bst6I CTCTTC 1 cut(s) 291
BstACI GRCGYC 1 cut(s) 434
BstAPI GCANNNNNTGC 1 cut(s) 167
BstDEI CTNAG 6 cut(s) 147, 339, 357, 438, 603, 630
BstF5I GGATG 4 cut(s) 91, 157, 517, 580
BstMAI GTCTC 2 cut(s) 441, 464
BstMWI GCNNNNNNNGC 2 cut(s) 167, 453
BstNI CCWGG 1 cut(s) 16
BstNSI RCATGY 2 cut(s) 35, 425
BstSCI CCNGG 1 cut(s) 14
BstV2I GAAGAC 1 cut(s) 204
BstXI CCANNNNNNTGG 1 cut(s) 111
Bsu36I CCTNAGG 1 cut(s) 147
BsuI GTATCC 1 cut(s) 12
BtsCI GGATG 4 cut(s) 91, 157, 517, 580
BtsIMutI CAGTG 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 403
CseI GACGC 1 cut(s) 423
Csp6I GTAC 2 cut(s) 498, 522
CviAII CATG 4 cut(s) 32, 210, 369, 422
CviJI RGCY 6 cut(s) 43, 183, 251, 305, 413, 634
CviKI_1 RGCY 6 cut(s) 43, 183, 251, 305, 413, 634
CviQI GTAC 2 cut(s) 498, 522
DdeI CTNAG 6 cut(s) 147, 339, 357, 438, 603, 630
Eam1104I CTCTTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 291
Ecl136II GAGCTC 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 185
Eco47I GGWCC 1 cut(s) 403
Eco53kI GAGCTC 1 cut(s) 183
Eco81I CCTNAGG 1 cut(s) 147
EcoICRI GAGCTC 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 14
EcoT38I GRGCYC 1 cut(s) 185
Esp3I CGTCTC 1 cut(s) 441
FaeI CATG 4 cut(s) 35, 213, 372, 425
FalI AAGNNNNNCTT 4 cut(s) 150, 182, 608, 640
FaqI GGGAC 2 cut(s) 416, 568
FatI CATG 4 cut(s) 31, 209, 368, 421
FauNDI CATATG 1 cut(s) 483
FokI GGATG 4 cut(s) 98, 164, 524, 587
FriOI GRGCYC 1 cut(s) 185
FspBI CTAG 5 cut(s) 186, 348, 399, 417, 551
HgaI GACGC 1 cut(s) 423
Hin1I GRCGYC 1 cut(s) 434
Hin1II CATG 4 cut(s) 35, 213, 372, 425
HincII GTYRAC 3 cut(s) 65, 207, 591
HindII GTYRAC 3 cut(s) 65, 207, 591
HinfI GANTC 3 cut(s) 61, 560, 654
HpaI GTTAAC 1 cut(s) 207
Hpy166II GTNNAC 4 cut(s) 65, 207, 237, 591
Hpy188I TCNGA 4 cut(s) 441, 565, 604, 659
Hpy188III TCNNGA 3 cut(s) 146, 186, 417
Hpy8I GTNNAC 4 cut(s) 65, 207, 237, 591
HpyAV CCTTC 2 cut(s) 274, 303
HpyCH4III ACNGT 3 cut(s) 241, 475, 612
HpyCH4IV ACGT 1 cut(s) 593
HpyCH4V TGCA 4 cut(s) 155, 368, 456, 467
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 453
HpyF3I CTNAG 6 cut(s) 147, 339, 357, 438, 603, 630
HpySE526I ACGT 1 cut(s) 593
Hsp92I GRCGYC 1 cut(s) 434
Hsp92II CATG 4 cut(s) 35, 213, 372, 425
KspAI GTTAAC 1 cut(s) 207
LpnPI CCDG 4 cut(s) 28, 159, 187, 207
LweI GCATC 5 cut(s) 142, 333, 454, 543, 565
MaeI CTAG 5 cut(s) 186, 348, 399, 417, 551
MaeII ACGT 1 cut(s) 593
MaeIII GTNAC 2 cut(s) 27, 361
MboII GAAGA 2 cut(s) 209, 278
MhlI GDGCHC 1 cut(s) 185
MluCI AATT 4 cut(s) 109, 327, 375, 493
MlyI GAGTC 1 cut(s) 70
MnlI CCTC 4 cut(s) 54, 111, 142, 300
MseI TTAA 1 cut(s) 206
MspR9I CCNGG 1 cut(s) 16
Mva1269I GAATGC 1 cut(s) 340
MvaI CCWGG 1 cut(s) 16
MwoI GCNNNNNNNGC 2 cut(s) 167, 453
NdeI CATATG 1 cut(s) 483
NlaIII CATG 4 cut(s) 35, 213, 372, 425
NlaIV GGNNCC 1 cut(s) 404
NspI RCATGY 2 cut(s) 35, 425
PciI ACATGT 1 cut(s) 421
PctI GAATGC 1 cut(s) 340
PfeI GAWTC 2 cut(s) 560, 654
PleI GAGTC 1 cut(s) 69
PpsI GAGTC 1 cut(s) 69
PscI ACATGT 1 cut(s) 421
Psp124BI GAGCTC 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 14
PspGI CCWGG 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 404
PspPI GGNCC 1 cut(s) 403
RsaI GTAC 2 cut(s) 499, 523
RsaNI GTAC 2 cut(s) 498, 522
SacI GAGCTC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 206
Sau96I GGNCC 1 cut(s) 403
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 1 cut(s) 16
SduI GDGCHC 1 cut(s) 185
SetI ASST 9 cut(s) 129, 185, 206, 232, 253, 363, 415, 596, 636
SfaNI GCATC 5 cut(s) 142, 333, 454, 543, 565
SinI GGWCC 1 cut(s) 403
SmlI CTYRAG 1 cut(s) 580
SmoI CTYRAG 1 cut(s) 580
Sse9I AATT 4 cut(s) 109, 327, 375, 493
SspMI CTAG 5 cut(s) 186, 348, 399, 417, 551
SstI GAGCTC 1 cut(s) 185
StyD4I CCNGG 1 cut(s) 14
TaaI ACNGT 3 cut(s) 241, 475, 612
TaiI ACGT 1 cut(s) 596
TaqII GACCGA 1 cut(s) 420
TasI AATT 4 cut(s) 109, 327, 375, 493
TatI WGTACW 1 cut(s) 497
TfiI GAWTC 2 cut(s) 560, 654
Tru1I TTAA 1 cut(s) 206
Tru9I TTAA 1 cut(s) 206
TscAI CASTG 1 cut(s) 244
TspDTI ATGAA 2 cut(s) 102, 470
TspRI CASTG 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 403
XapI RAATTY 2 cut(s) 327, 375
XbaI TCTAGA 2 cut(s) 185, 416
XceI RCATGY 2 cut(s) 35, 425
XspI CTAG 5 cut(s) 186, 348, 399, 417, 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.