pycom16g16110

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010926
Physical Location & Seq
Reverse (-)
12052141 .. 12055029
2889 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g16110.8

Sequence Viewer

Length: 1032 bp
ATGTTTTGTGTTGTGTCACTTGGGCGAGTGTTGTTGCCTCGCTATTCCCTCTGCGGTGTGATGTGGCCTCGCTATTTAGCAGTGACTAATCGCTCTCACTTCTCCCCATTGTATTTCGTGATTTGTAGAAGCAAAATAAACCTTAATTTTCACGGCCTTCTTAACCGGTTGAGTTACAAATTTTTTACTTTTTTGAAAATCGATTTCAGCTCTCGTCTCTCTAAGCTTCCCATGGATTCCCGCAGTTCCTCTTCTCCTTTTGATTCCATCATTTTCGATTTGGATGACACTCTGTACTCTTCCAACCTTGGACTCGGTGAAGCTTGCAAGAAGAACATCGATGATTTTCTCGTTGAAAAATGCGGATTCCCAGAGAGCAAGGCGTCCGGCCTTCGCGTTGAGCTTTTCAAAAAATACGGCAGCTCCCTCGCTGGCTTACGAGCGTTGGGGTACGATATCGACGCCGAAGATTACCACGACGTCGTGCATGGAAGGCTGCCGTACGATCGGATCAAACCCGACTCTCAGCTCCGAGACCTCCTCCGCAGCATCTCGCAAAGAAAAATCATATTTACGAATTCGGACCGGAAGCACGCGGTGAAGGTGTTGGAACGTCTGGGAGTGGAGGAGTGCTTCGATCGGATCATATGCTTCGAGACAATGAACCCAAACCTTCCAAGTTCGACTCAGCCGGACGAGTTCCCGGTGGTCCTGAAGCCGTCCATAGAGGCCATGGAGATCGCGCTCCGGGCTGCGGAGGTCGATCCTCGCCGCACGCTGTTTCTCGATGACAACGTGCGTAACGTCGCCGCGGGGAAAGCTGTTGGTCTGCGCACCGTTTTGGTTGGAAAAACCGTGAAAAGCAAGGAAGCAGATTACGTGTTAGAGAATGTGAACAACATGGCACAAGCAATATCAGAAGTATGGGTGGGAGGAAGCGGCCCAAAGGATGGTAGCAACCAAAGGATCAGCCGCACCCGAAGCGACATCGAGCTCGATTCCGTCCTCACCGCCACAGCTGTCGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase Hydrolase_like

Protein Analysis

344

Amino Acids

38.42

Weight (kDa)

8.58

Isoelectric Point (pI)

40.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32150 AT2G32150 AT2G32150
fragaria_vesca FvH4_4g15270 FvH4_4g15270
malus_domestica MD13G1191400.v1.1 MD16G1191500.v1.1
prunus_persica Prupe.1G011600_v2.0.a1
pyrus_communis pycom13g16420 pycom16g16110
rosa_chinensis RchiOBHm_Chr4g0416551
rosa_laevigata RLG00000007974
rosa_multiflora Rmu_sc0003722.1_g000037
rosa_roxburghii Rroxscaffold_5G00360440
rosa_rugosa Rorug04G0142800
rosa_samantha Rh4AG205300 Rh4BG202900 Rh4CG216200 Rh4DG202400
rosa_wichuraiana Rw4G017380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 483
Acc16I TGCGCA 1 cut(s) 833
AccB7I CCANNNNNTGG 1 cut(s) 950
AccII CGCG 4 cut(s) 396, 596, 743, 812
AclWI GGATC 4 cut(s) 518, 650, 758, 974
AcsI RAATTY 2 cut(s) 179, 577
AcuI CTGAAG 1 cut(s) 734
AcyI GRCGYC 3 cut(s) 383, 462, 480
AdeI CACNNNGTG 1 cut(s) 598
AfaI GTAC 3 cut(s) 296, 452, 503
AfiI CCNNNNNNNGG 2 cut(s) 754, 950
AflIII ACRYGT 1 cut(s) 879
AgeI ACCGGT 1 cut(s) 165
AgsI TTSAA 3 cut(s) 196, 356, 409
Alw21I GWGCWC 1 cut(s) 996
Alw26I GTCTC 3 cut(s) 221, 528, 650
AlwI GGATC 4 cut(s) 518, 650, 758, 974
AoxI GGCC 5 cut(s) 65, 154, 388, 729, 940
ApeKI GCWGC 4 cut(s) 420, 496, 546, 752
ApoI RAATTY 2 cut(s) 179, 577
AsiGI ACCGGT 1 cut(s) 165
AspLEI GCGC 2 cut(s) 745, 834
AspS9I GGNCC 3 cut(s) 583, 709, 941
AsuC2I CCSGG 2 cut(s) 704, 749
AsuHPI GGTGA 3 cut(s) 329, 610, 1000
AvaII GGWCC 2 cut(s) 583, 709
BanII GRGCYC 1 cut(s) 996
Bbv12I GWGCWC 1 cut(s) 996
BbvI GCAGC 4 cut(s) 432, 483, 558, 739
BccI CCATC 2 cut(s) 275, 944
BceAI ACGGC 4 cut(s) 169, 433, 484, 703
BcgI CGANNNNNNTGC 2 cut(s) 409, 443
BcnI CCSGG 2 cut(s) 704, 749
BcoDI GTCTC 3 cut(s) 221, 528, 650
BisI GCNGC 8 cut(s) 421, 497, 547, 753, 772, 810, 940, 973
BlsI GCNGC 8 cut(s) 422, 498, 548, 754, 773, 811, 941, 974
Bme1390I CCNGG 2 cut(s) 704, 749
Bme18I GGWCC 2 cut(s) 583, 709
BmgT120I GGNCC 3 cut(s) 583, 709, 941
BmrFI CCNGG 2 cut(s) 704, 749
BmsI GCATC 1 cut(s) 558
BplI GAGNNNNNCTC 2 cut(s) 525, 557
BpuMI CCSGG 2 cut(s) 704, 749
Bsa29I ATCGAT 2 cut(s) 201, 339
BsaAI YACGTR 1 cut(s) 880
BsaHI GRCGYC 3 cut(s) 383, 462, 480
BsaI GGTCTC 1 cut(s) 528
BsaJI CCNNGG 4 cut(s) 231, 307, 732, 810
BsaWI WCCGGW 2 cut(s) 165, 585
BsaXI ACNNNNNCTCC 2 cut(s) 407, 437
Bsc4I CCNNNNNNNGG 2 cut(s) 754, 950
Bse118I RCCGGY 1 cut(s) 165
BseCI ATCGAT 2 cut(s) 201, 339
BseDI CCNNGG 4 cut(s) 231, 307, 732, 810
BseGI GGATG 2 cut(s) 289, 955
BseLI CCNNNNNNNGG 2 cut(s) 754, 950
BseMII CTCAG 2 cut(s) 539, 701
BseRI GAGGAG 2 cut(s) 530, 641
BseXI GCAGC 4 cut(s) 432, 483, 558, 739
Bsh1236I CGCG 4 cut(s) 396, 596, 743, 812
Bsh1285I CGRYCG 2 cut(s) 508, 640
BshFI GGCC 5 cut(s) 67, 156, 390, 731, 942
BshTI ACCGGT 1 cut(s) 165
BshVI ATCGAT 2 cut(s) 201, 339
BsiEI CGRYCG 2 cut(s) 508, 640
BsiHKAI GWGCWC 1 cut(s) 996
BsiSI CCGG 6 cut(s) 166, 387, 586, 692, 704, 748
BsiWI CGTACG 1 cut(s) 501
BslI CCNNNNNNNGG 2 cut(s) 754, 950
BsmAI GTCTC 3 cut(s) 221, 528, 650
BsmBI CGTCTC 1 cut(s) 221
BsnI GGCC 5 cut(s) 67, 156, 390, 731, 942
Bso31I GGTCTC 1 cut(s) 528
Bsp1286I GDGCHC 1 cut(s) 996
Bsp143I GATC 7 cut(s) 505, 510, 637, 642, 738, 763, 966
Bsp19I CCATGG 2 cut(s) 231, 732
BspANI GGCC 5 cut(s) 67, 156, 390, 731, 942
BspCNI CTCAG 2 cut(s) 538, 700
BspDI ATCGAT 2 cut(s) 201, 339
BspFNI CGCG 4 cut(s) 396, 596, 743, 812
BspPI GGATC 4 cut(s) 518, 650, 758, 974
BspTNI GGTCTC 1 cut(s) 528
BsrFI RCCGGY 1 cut(s) 165
BssAI RCCGGY 1 cut(s) 165
BssECI CCNNGG 4 cut(s) 231, 307, 732, 810
BssMI GATC 7 cut(s) 505, 510, 637, 642, 738, 763, 966
BssNI GRCGYC 3 cut(s) 383, 462, 480
BssT1I CCWWGG 3 cut(s) 231, 307, 732
Bst4CI ACNGT 2 cut(s) 838, 856
Bst6I CTCTTC 2 cut(s) 256, 304
BstACI GRCGYC 3 cut(s) 383, 462, 480
BstBAI YACGTR 1 cut(s) 880
BstC8I GCNNGC 4 cut(s) 325, 433, 594, 776
BstDEI CTNAG 3 cut(s) 222, 525, 687
BstDSI CCRYGG 3 cut(s) 231, 732, 810
BstF5I GGATG 2 cut(s) 289, 955
BstFNI CGCG 4 cut(s) 396, 596, 743, 812
BstHHI GCGC 2 cut(s) 745, 834
BstKTI GATC 7 cut(s) 508, 513, 640, 645, 741, 766, 969
BstMAI GTCTC 3 cut(s) 221, 528, 650
BstMBI GATC 7 cut(s) 505, 510, 637, 642, 738, 763, 966
BstMCI CGRYCG 2 cut(s) 508, 640
BstMWI GCNNNNNNNGC 5 cut(s) 493, 749, 818, 981, 1025
BstSCI CCNGG 2 cut(s) 702, 747
BstUI CGCG 4 cut(s) 396, 596, 743, 812
BstV1I GCAGC 4 cut(s) 432, 483, 558, 739
Bsu15I ATCGAT 2 cut(s) 201, 339
BsuRI GGCC 5 cut(s) 67, 156, 390, 731, 942
BsuTUI ATCGAT 2 cut(s) 201, 339
BtgI CCRYGG 3 cut(s) 231, 732, 810
BtsCI GGATG 2 cut(s) 289, 955
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 87
Cac8I GCNNGC 4 cut(s) 325, 433, 594, 776
CfoI GCGC 2 cut(s) 745, 834
Cfr10I RCCGGY 1 cut(s) 165
Cfr13I GGNCC 3 cut(s) 583, 709, 941
Cfr42I CCGCGG 1 cut(s) 813
ClaI ATCGAT 2 cut(s) 201, 339
CpoI CGGWCCG 1 cut(s) 583
CseI GACGC 2 cut(s) 372, 470
Csp6I GTAC 3 cut(s) 295, 451, 502
CspAI ACCGGT 1 cut(s) 165
CspI CGGWCCG 1 cut(s) 583
CviAII CATG 4 cut(s) 232, 488, 733, 901
CviQI GTAC 3 cut(s) 295, 451, 502
DdeI CTNAG 3 cut(s) 222, 525, 687
DpnI GATC 7 cut(s) 507, 512, 639, 644, 740, 765, 968
DpnII GATC 7 cut(s) 505, 510, 637, 642, 738, 763, 966
DraIII CACNNNGTG 1 cut(s) 598
Eam1104I CTCTTC 2 cut(s) 256, 304
EarI CTCTTC 2 cut(s) 256, 304
Ecl136II GAGCTC 1 cut(s) 994
Eco130I CCWWGG 3 cut(s) 231, 307, 732
Eco24I GRGCYC 1 cut(s) 996
Eco31I GGTCTC 1 cut(s) 528
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 2 cut(s) 583, 709
Eco53kI GAGCTC 1 cut(s) 994
Eco57I CTGAAG 1 cut(s) 734
EcoICRI GAGCTC 1 cut(s) 994
EcoRI GAATTC 1 cut(s) 577
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 3 cut(s) 231, 307, 732
EcoT38I GRGCYC 1 cut(s) 996
ErhI CCWWGG 3 cut(s) 231, 307, 732
Esp3I CGTCTC 1 cut(s) 221
FaeI CATG 4 cut(s) 235, 491, 736, 904
FaiI YATR 9 cut(s) 233, 489, 569, 647, 649, 725, 734, 902, 925
FatI CATG 4 cut(s) 231, 487, 732, 900
FauI CCCGC 2 cut(s) 248, 805
FauNDI CATATG 1 cut(s) 647
Fnu4HI GCNGC 8 cut(s) 421, 497, 547, 753, 772, 810, 940, 973
FokI GGATG 2 cut(s) 296, 962
FriOI GRGCYC 1 cut(s) 996
Fsp4HI GCNGC 8 cut(s) 421, 497, 547, 753, 772, 810, 940, 973
FspI TGCGCA 1 cut(s) 833
GlaI GCGC 2 cut(s) 744, 833
GluI GCNGC 8 cut(s) 421, 497, 547, 753, 772, 810, 940, 973
HaeIII GGCC 5 cut(s) 67, 156, 390, 731, 942
HapII CCGG 6 cut(s) 166, 387, 586, 692, 704, 748
HgaI GACGC 2 cut(s) 372, 470
HhaI GCGC 2 cut(s) 745, 834
Hin1I GRCGYC 3 cut(s) 383, 462, 480
Hin1II CATG 4 cut(s) 235, 491, 736, 904
Hin6I GCGC 2 cut(s) 743, 832
HinP1I GCGC 2 cut(s) 743, 832
HindIII AAGCTT 2 cut(s) 224, 321
HinfI GANTC 7 cut(s) 236, 263, 312, 366, 521, 685, 998
HpaII CCGG 6 cut(s) 166, 387, 586, 692, 704, 748
HphI GGTGA 3 cut(s) 329, 610, 1000
Hpy166II GTNNAC 1 cut(s) 895
Hpy188I TCNGA 6 cut(s) 510, 533, 583, 642, 919, 1025
Hpy188III TCNNGA 4 cut(s) 118, 655, 712, 785
Hpy8I GTNNAC 1 cut(s) 895
Hpy99I CGWCG 4 cut(s) 464, 482, 485, 809
HpyAV CCTTC 5 cut(s) 167, 401, 486, 595, 683
HpyCH4III ACNGT 2 cut(s) 838, 856
HpyCH4IV ACGT 5 cut(s) 480, 613, 795, 804, 879
HpyCH4V TGCA 2 cut(s) 327, 487
HpyF10VI GCNNNNNNNGC 5 cut(s) 493, 749, 818, 981, 1025
HpyF3I CTNAG 3 cut(s) 222, 525, 687
HpySE526I ACGT 5 cut(s) 480, 613, 795, 804, 879
Hsp92I GRCGYC 3 cut(s) 383, 462, 480
Hsp92II CATG 4 cut(s) 235, 491, 736, 904
HspAI GCGC 2 cut(s) 743, 832
KspI CCGCGG 1 cut(s) 813
Kzo9I GATC 7 cut(s) 505, 510, 637, 642, 738, 763, 966
LmnI GCTCC 4 cut(s) 428, 534, 750, 1025
Lsp1109I GCAGC 4 cut(s) 432, 483, 558, 739
LweI GCATC 1 cut(s) 558
MaeII ACGT 5 cut(s) 480, 613, 795, 804, 879
MaeIII GTNAC 4 cut(s) 15, 82, 173, 800
MalI GATC 7 cut(s) 507, 512, 639, 644, 740, 765, 968
MboI GATC 7 cut(s) 505, 510, 637, 642, 738, 763, 966
MboII GAAGA 4 cut(s) 243, 291, 343, 479
MhlI GDGCHC 1 cut(s) 996
MluCI AATT 3 cut(s) 145, 179, 577
MlyI GAGTC 3 cut(s) 306, 515, 679
MmeI TCCRAC 4 cut(s) 327, 588, 826, 1003
MseI TTAA 2 cut(s) 144, 162
MspA1I CMGCKG 2 cut(s) 812, 1019
MspI CCGG 6 cut(s) 166, 387, 586, 692, 704, 748
MspR9I CCNGG 2 cut(s) 704, 749
MvnI CGCG 4 cut(s) 396, 596, 743, 812
MwoI GCNNNNNNNGC 5 cut(s) 493, 749, 818, 981, 1025
NciI CCSGG 2 cut(s) 704, 749
NcoI CCATGG 2 cut(s) 231, 732
NdeI CATATG 1 cut(s) 647
NdeII GATC 7 cut(s) 505, 510, 637, 642, 738, 763, 966
NlaIII CATG 4 cut(s) 235, 491, 736, 904
NmuCI GTSAC 2 cut(s) 15, 82
NsbI TGCGCA 1 cut(s) 833
PcsI WCGNNNNNNNCGW 2 cut(s) 792, 801
PfeI GAWTC 4 cut(s) 236, 263, 366, 998
Pfl23II CGTACG 1 cut(s) 501
PflMI CCANNNNNTGG 1 cut(s) 950
PinAI ACCGGT 1 cut(s) 165
PkrI GCNGC 8 cut(s) 422, 498, 548, 754, 773, 811, 941, 974
Ple19I CGATCG 2 cut(s) 508, 640
PleI GAGTC 3 cut(s) 306, 515, 679
PpsI GAGTC 3 cut(s) 306, 515, 679
Ppu21I YACGTR 1 cut(s) 880
Psp124BI GAGCTC 1 cut(s) 996
PspLI CGTACG 1 cut(s) 501
PspPI GGNCC 3 cut(s) 583, 709, 941
PvuI CGATCG 2 cut(s) 508, 640
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 3 cut(s) 296, 452, 503
RsaNI GTAC 3 cut(s) 295, 451, 502
Rsr2I CGGWCCG 1 cut(s) 583
RsrII CGGWCCG 1 cut(s) 583
SacI GAGCTC 1 cut(s) 996
SacII CCGCGG 1 cut(s) 813
SaqAI TTAA 2 cut(s) 144, 162
SatI GCNGC 8 cut(s) 421, 497, 547, 753, 772, 810, 940, 973
Sau3AI GATC 7 cut(s) 505, 510, 637, 642, 738, 763, 966
Sau96I GGNCC 3 cut(s) 583, 709, 941
SchI GAGTC 3 cut(s) 306, 515, 679
ScrFI CCNGG 2 cut(s) 704, 749
SduI GDGCHC 1 cut(s) 996
SfaNI GCATC 1 cut(s) 558
Sfr303I CCGCGG 1 cut(s) 813
SgrBI CCGCGG 1 cut(s) 813
SinI GGWCC 2 cut(s) 583, 709
Sse9I AATT 3 cut(s) 145, 179, 577
SstI GAGCTC 1 cut(s) 996
StyD4I CCNGG 2 cut(s) 702, 747
StyI CCWWGG 3 cut(s) 231, 307, 732
TaaI ACNGT 2 cut(s) 838, 856
TaiI ACGT 5 cut(s) 483, 616, 798, 807, 882
TasI AATT 3 cut(s) 145, 179, 577
TatI WGTACW 1 cut(s) 294
TauI GCSGC 4 cut(s) 774, 812, 942, 975
TfiI GAWTC 4 cut(s) 236, 263, 366, 998
Tru1I TTAA 2 cut(s) 144, 162
Tru9I TTAA 2 cut(s) 144, 162
TscAI CASTG 1 cut(s) 87
TseFI GTSAC 2 cut(s) 15, 82
TseI GCWGC 4 cut(s) 420, 496, 546, 752
Tsp45I GTSAC 2 cut(s) 15, 82
TspDTI ATGAA 1 cut(s) 677
TspGWI ACGGA 1 cut(s) 991
TspRI CASTG 1 cut(s) 87
Van91I CCANNNNNTGG 1 cut(s) 950
VpaK11BI GGWCC 2 cut(s) 583, 709
XapI RAATTY 2 cut(s) 179, 577
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
ZraI GACGTC 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.