Prupe.1G011600_v2.0.a1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0010926
Physical Location & Seq
Reverse (-)
892267 .. 894542
2276 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G011600.1

Sequence Viewer

Length: 810 bp
ATGGATTCCTGCAGTTCCTCTGTTCGCGATTCTTCTTCTCCTTTCGATTGCCTCATCTTCGATTTGGACGACACTCTGTACCCTTCCAACACTGGATTAGGTGAAGCTTGCAAGACGAACATCGATGATTTTCTAGTTGAGAAATGCGGGTTCCCGGAGAGCAAAGCCTCCAGTCTTCGAGTTGAGCTTTTCAAAAGCTACGGCAGCACCCTCGCCGGCTTACGTGCGTTAGGCTACGACATCGACGCCGACGATTACCACAGTGTCGTGCACGGAAGGTTGCCGTACGATCGGATCAAGCCCGATCCTCAACTCCGAAACCTCCTCCGTAGCATCGCACAGAGAAAAATCATATTTACGAATTCGGATAGGAAACACGCGGTGAAGGCGTTGGATCGTCTGGGAGTGAGAGACTGCTTCGAGCAGATCATATGCTTCGAGACGATGAACCCGAACCTGCCGAGTTCGACTCGGCCTAACGAGTTTCCGGTGGTTCTGAAGCCGTCCATGGAGGCCATGGAGATAGCGCTCCGGGTCGCCGAGGTGGATCCTCGTCGTACGCTGTTTCTCGATGACAATATCCGTAACGTCGCTGCCGGGAAAGCCGTGGGTCTACGCACCGTTTTGGTTGGAAAAACCGTGAAAAGCAATGAAGCAGATTACGTGTTAGAGAACGTGAACAACCTGGCACAAGCAGTTTCAGAAGTATGGGTAGGCGGAAAAGATGGTGGTAGTAACAAAAGGATCAGCCGCACCAGAAGCGACATTGAGGTCGATTCCATTCTCACAACCACAGCTGTTGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase Hydrolase_like

Protein Analysis

270

Amino Acids

29.64

Weight (kDa)

5.54

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32150 AT2G32150 AT2G32150
fragaria_vesca FvH4_4g15270 FvH4_4g15270
malus_domestica MD13G1191400.v1.1 MD16G1191500.v1.1
prunus_persica Prupe.1G011600_v2.0.a1
pyrus_communis pycom13g16420 pycom16g16110
rosa_chinensis RchiOBHm_Chr4g0416551
rosa_laevigata RLG00000007974
rosa_multiflora Rmu_sc0003722.1_g000037
rosa_roxburghii Rroxscaffold_5G00360440
rosa_rugosa Rorug04G0142800
rosa_samantha Rh4AG205300 Rh4BG202900 Rh4CG216200 Rh4DG202400
rosa_wichuraiana Rw4G017380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 770
Acc36I ACCTGC 1 cut(s) 465
AccI GTMKAC 1 cut(s) 613
AccII CGCG 2 cut(s) 27, 380
AciI CCGC 4 cut(s) 147, 380, 717, 751
AclWI GGATC 6 cut(s) 299, 302, 402, 542, 555, 752
AcsI RAATTY 1 cut(s) 361
AcuI CTGAAG 1 cut(s) 518
AcyI GRCGYC 1 cut(s) 246
AdeI CACNNNGTG 1 cut(s) 382
AfaI GTAC 3 cut(s) 80, 287, 559
AfeI AGCGCT 1 cut(s) 528
AflIII ACRYGT 1 cut(s) 663
AgsI TTSAA 1 cut(s) 193
AjnI CCWGG 1 cut(s) 684
AluBI AGCT 5 cut(s) 107, 187, 198, 797, 806
AluI AGCT 5 cut(s) 107, 187, 198, 797, 806
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 2 cut(s) 405, 434
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 6 cut(s) 299, 302, 402, 542, 555, 752
Aor51HI AGCGCT 1 cut(s) 528
AoxI GGCC 2 cut(s) 473, 513
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 2 cut(s) 204, 593
ApoI RAATTY 1 cut(s) 361
AspLEI GCGC 1 cut(s) 529
AsuC2I CCSGG 3 cut(s) 155, 533, 598
AsuHPI GGTGA 2 cut(s) 113, 394
BaeGI GKGCMC 1 cut(s) 273
BamHI GGATCC 1 cut(s) 547
BbsI GAAGAC 1 cut(s) 167
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 216, 580
BccI CCATC 1 cut(s) 719
BceAI ACGGC 4 cut(s) 217, 268, 487, 590
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BciT130I CCWGG 1 cut(s) 686
BcnI CCSGG 3 cut(s) 155, 533, 598
BcoDI GTCTC 2 cut(s) 405, 434
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 10
BfoI RGCGCY 1 cut(s) 530
BfuAI ACCTGC 1 cut(s) 465
BisI GCNGC 3 cut(s) 205, 594, 751
BlsI GCNGC 3 cut(s) 206, 595, 752
Bme1390I CCNGG 4 cut(s) 155, 533, 598, 686
BmiI GGNNCC 2 cut(s) 152, 549
BmrFI CCNGG 4 cut(s) 155, 533, 598, 686
BmsI GCATC 1 cut(s) 342
BpiI GAAGAC 1 cut(s) 167
BplI GAGNNNNNCTC 2 cut(s) 454, 486
BpmI CTGGAG 1 cut(s) 154
BpuMI CCSGG 3 cut(s) 155, 533, 598
Bsa29I ATCGAT 1 cut(s) 123
BsaAI YACGTR 2 cut(s) 224, 664
BsaHI GRCGYC 1 cut(s) 246
BsaJI CCNNGG 4 cut(s) 507, 516, 540, 606
BsaWI WCCGGW 1 cut(s) 487
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 97, 171
Bse3DI GCAATG 1 cut(s) 655
BseBI CCWGG 1 cut(s) 686
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 4 cut(s) 507, 516, 540, 606
BseMI GCAATG 1 cut(s) 655
BseNI ACTGG 2 cut(s) 97, 171
BseRI GAGGAG 1 cut(s) 314
BseSI GKGCMC 1 cut(s) 273
BseXI GCAGC 2 cut(s) 216, 580
Bsh1236I CGCG 2 cut(s) 27, 380
Bsh1285I CGRYCG 1 cut(s) 292
BshFI GGCC 2 cut(s) 475, 515
BshVI ATCGAT 1 cut(s) 123
BsiEI CGRYCG 1 cut(s) 292
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 5 cut(s) 155, 216, 488, 532, 597
BsiWI CGTACG 2 cut(s) 285, 557
BsmAI GTCTC 2 cut(s) 405, 434
BsmBI CGTCTC 1 cut(s) 434
BsnI GGCC 2 cut(s) 475, 515
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 7 cut(s) 289, 294, 304, 394, 426, 547, 744
Bsp19I CCATGG 2 cut(s) 507, 516
Bsp68I TCGCGA 1 cut(s) 27
BspACI CCGC 4 cut(s) 147, 380, 717, 751
BspANI GGCC 2 cut(s) 475, 515
BspDI ATCGAT 1 cut(s) 123
BspFNI CGCG 2 cut(s) 27, 380
BspLI GGNNCC 2 cut(s) 152, 549
BspMAI CTGCAG 1 cut(s) 14
BspMI ACCTGC 1 cut(s) 465
BspPI GGATC 6 cut(s) 299, 302, 402, 542, 555, 752
BsrDI GCAATG 1 cut(s) 655
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 2 cut(s) 97, 171
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 4 cut(s) 507, 516, 540, 606
BssMI GATC 7 cut(s) 289, 294, 304, 394, 426, 547, 744
BssNI GRCGYC 1 cut(s) 246
BssT1I CCWWGG 2 cut(s) 507, 516
Bst2UI CCWGG 1 cut(s) 686
Bst4CI ACNGT 3 cut(s) 263, 622, 640
BstACI GRCGYC 1 cut(s) 246
BstBAI YACGTR 2 cut(s) 224, 664
BstC8I GCNNGC 2 cut(s) 109, 217
BstDSI CCRYGG 3 cut(s) 507, 516, 606
BstFNI CGCG 2 cut(s) 27, 380
BstH2I RGCGCY 1 cut(s) 530
BstHHI GCGC 1 cut(s) 529
BstKTI GATC 7 cut(s) 292, 297, 307, 397, 429, 550, 747
BstMAI GTCTC 2 cut(s) 405, 434
BstMBI GATC 7 cut(s) 289, 294, 304, 394, 426, 547, 744
BstMCI CGRYCG 1 cut(s) 292
BstMWI GCNNNNNNNGC 5 cut(s) 204, 386, 602, 759, 803
BstNI CCWGG 1 cut(s) 686
BstSCI CCNGG 4 cut(s) 153, 531, 596, 684
BstSFI CTRYAG 1 cut(s) 10
BstSLI GKGCMC 1 cut(s) 273
BstUI CGCG 2 cut(s) 27, 380
BstV1I GCAGC 2 cut(s) 216, 580
BstV2I GAAGAC 1 cut(s) 167
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 2 cut(s) 475, 515
BsuTUI ATCGAT 1 cut(s) 123
BtgI CCRYGG 3 cut(s) 507, 516, 606
BtgZI GCGATG 1 cut(s) 319
BtsIMutI CAGTG 2 cut(s) 90, 268
BtuMI TCGCGA 1 cut(s) 27
BveI ACCTGC 1 cut(s) 465
Cac8I GCNNGC 2 cut(s) 109, 217
CfoI GCGC 1 cut(s) 529
Cfr10I RCCGGY 1 cut(s) 215
ClaI ATCGAT 1 cut(s) 123
CseI GACGC 1 cut(s) 254
Csp6I GTAC 3 cut(s) 79, 286, 558
CspCI CAANNNNNGTGG 1 cut(s) 781
CviAII CATG 2 cut(s) 508, 517
CviQI GTAC 3 cut(s) 79, 286, 558
DpnI GATC 7 cut(s) 291, 296, 306, 396, 428, 549, 746
DpnII GATC 7 cut(s) 289, 294, 304, 394, 426, 547, 744
DraIII CACNNNGTG 1 cut(s) 382
DrdI GACNNNNNNGTC 1 cut(s) 770
DseDI GACNNNNNNGTC 1 cut(s) 770
EciI GGCGGA 1 cut(s) 732
Eco130I CCWWGG 2 cut(s) 507, 516
Eco47III AGCGCT 1 cut(s) 528
Eco57I CTGAAG 1 cut(s) 518
EcoRI GAATTC 1 cut(s) 361
EcoRII CCWGG 1 cut(s) 684
EcoT14I CCWWGG 2 cut(s) 507, 516
ErhI CCWWGG 2 cut(s) 507, 516
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 2 cut(s) 511, 520
FaiI YATR 6 cut(s) 353, 431, 433, 509, 518, 709
FatI CATG 2 cut(s) 507, 516
FauI CCCGC 1 cut(s) 140
FauNDI CATATG 1 cut(s) 431
FblI GTMKAC 1 cut(s) 613
Fnu4HI GCNGC 3 cut(s) 205, 594, 751
Fsp4HI GCNGC 3 cut(s) 205, 594, 751
FspBI CTAG 1 cut(s) 134
GlaI GCGC 1 cut(s) 528
GluI GCNGC 3 cut(s) 205, 594, 751
GsuI CTGGAG 1 cut(s) 154
HaeII RGCGCY 1 cut(s) 530
HaeIII GGCC 2 cut(s) 475, 515
HapII CCGG 5 cut(s) 155, 216, 488, 532, 597
HgaI GACGC 1 cut(s) 254
HhaI GCGC 1 cut(s) 529
Hin1I GRCGYC 1 cut(s) 246
Hin1II CATG 2 cut(s) 511, 520
Hin6I GCGC 1 cut(s) 527
HinP1I GCGC 1 cut(s) 527
HindIII AAGCTT 1 cut(s) 105
HinfI GANTC 4 cut(s) 5, 29, 469, 776
HpaII CCGG 5 cut(s) 155, 216, 488, 532, 597
HphI GGTGA 2 cut(s) 113, 394
Hpy166II GTNNAC 3 cut(s) 271, 614, 679
Hpy188I TCNGA 5 cut(s) 294, 317, 367, 498, 703
Hpy188III TCNNGA 3 cut(s) 26, 439, 569
Hpy8I GTNNAC 3 cut(s) 271, 614, 679
Hpy99I CGWCG 4 cut(s) 248, 254, 558, 593
HpyAV CCTTC 3 cut(s) 93, 270, 379
HpyCH4III ACNGT 3 cut(s) 263, 622, 640
HpyCH4IV ACGT 4 cut(s) 223, 588, 663, 675
HpyCH4V TGCA 3 cut(s) 12, 111, 271
HpyF10VI GCNNNNNNNGC 5 cut(s) 204, 386, 602, 759, 803
HpySE526I ACGT 4 cut(s) 223, 588, 663, 675
Hsp92I GRCGYC 1 cut(s) 246
Hsp92II CATG 2 cut(s) 511, 520
HspAI GCGC 1 cut(s) 527
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Kzo9I GATC 7 cut(s) 289, 294, 304, 394, 426, 547, 744
LmnI GCTCC 2 cut(s) 534, 803
Lsp1109I GCAGC 2 cut(s) 216, 580
LweI GCATC 1 cut(s) 342
MaeI CTAG 1 cut(s) 134
MaeII ACGT 4 cut(s) 223, 588, 663, 675
MaeIII GTNAC 2 cut(s) 584, 734
MalI GATC 7 cut(s) 291, 296, 306, 396, 428, 549, 746
MboI GATC 7 cut(s) 289, 294, 304, 394, 426, 547, 744
MboII GAAGA 4 cut(s) 24, 27, 49, 167
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 1 cut(s) 361
MlyI GAGTC 1 cut(s) 463
MmeI TCCRAC 4 cut(s) 111, 372, 610, 781
MroNI GCCGGC 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 797
MspI CCGG 5 cut(s) 155, 216, 488, 532, 597
MspR9I CCNGG 4 cut(s) 155, 533, 598, 686
MvaI CCWGG 1 cut(s) 686
MvnI CGCG 2 cut(s) 27, 380
MwoI GCNNNNNNNGC 5 cut(s) 204, 386, 602, 759, 803
NaeI GCCGGC 1 cut(s) 217
NciI CCSGG 3 cut(s) 155, 533, 598
NcoI CCATGG 2 cut(s) 507, 516
NdeI CATATG 1 cut(s) 431
NdeII GATC 7 cut(s) 289, 294, 304, 394, 426, 547, 744
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 2 cut(s) 511, 520
NlaIV GGNNCC 2 cut(s) 152, 549
NmeAIII GCCGAG 3 cut(s) 451, 486, 565
NruI TCGCGA 1 cut(s) 27
PcsI WCGNNNNNNNCGW 3 cut(s) 66, 249, 449
PdiI GCCGGC 1 cut(s) 217
PfeI GAWTC 3 cut(s) 5, 29, 776
Pfl23II CGTACG 2 cut(s) 285, 557
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 3 cut(s) 206, 595, 752
Ple19I CGATCG 1 cut(s) 292
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
Ppu21I YACGTR 2 cut(s) 224, 664
Psp6I CCWGG 1 cut(s) 684
PspGI CCWGG 1 cut(s) 684
PspLI CGTACG 2 cut(s) 285, 557
PspN4I GGNNCC 2 cut(s) 152, 549
PstI CTGCAG 1 cut(s) 14
PsuI RGATCY 1 cut(s) 547
PvuI CGATCG 1 cut(s) 292
PvuII CAGCTG 1 cut(s) 797
RruI TCGCGA 1 cut(s) 27
RsaI GTAC 3 cut(s) 80, 287, 559
RsaNI GTAC 3 cut(s) 79, 286, 558
SatI GCNGC 3 cut(s) 205, 594, 751
Sau3AI GATC 7 cut(s) 289, 294, 304, 394, 426, 547, 744
SchI GAGTC 1 cut(s) 463
ScrFI CCNGG 4 cut(s) 155, 533, 598, 686
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 1 cut(s) 342
SfcI CTRYAG 1 cut(s) 10
Sse9I AATT 1 cut(s) 361
SsiI CCGC 4 cut(s) 147, 380, 717, 751
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 4 cut(s) 153, 531, 596, 684
StyI CCWWGG 2 cut(s) 507, 516
TaaI ACNGT 3 cut(s) 263, 622, 640
TaiI ACGT 4 cut(s) 226, 591, 666, 678
TasI AATT 1 cut(s) 361
TauI GCSGC 1 cut(s) 753
TfiI GAWTC 3 cut(s) 5, 29, 776
TscAI CASTG 2 cut(s) 97, 268
TseI GCWGC 2 cut(s) 204, 593
TspDTI ATGAA 2 cut(s) 461, 666
TspGWI ACGGA 3 cut(s) 288, 317, 572
TspRI CASTG 2 cut(s) 97, 268
VneI GTGCAC 1 cut(s) 269
XapI RAATTY 1 cut(s) 361
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 613
XspI CTAG 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.