FvH4_4g15270

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010926
Physical Location & Seq
Reverse (-)
18888855 .. 18891158
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g15270.t2

Sequence Viewer

Length: 807 bp
ATGGATTCCTGCAACTCCTTTCTTCGTGCTTCCTCCTCTCCTTTCGATTGCCTCATCTTCGATTTAGATGACACTTTGTACTCTTCAAGCATCGGACTCGGTGAAGCTCTCAAGAAGAACATCGATGATTTTCTTGTTGAAAAATGCGGGTTCCCGGAGAGCAAAGCTTCAAGCCTACGAGTTGAGCTTTTCAAAAAGTACGGCAGCTCCCTCGCCGGATTACGAGCGTTAGGCTACGACATTGATGCGGACGATTACCACAGTTTCGTGCACGGAAGGTTGCCTTATGATCGAATCAAACCCGATCATCAGCTACGGAACCTCATTCGCAGCATCCCACAGAGAAAAATCATTTTTACGAACTCGGACAGGAACCACGCGTTGACATGTTTGGATCGTCTGGGGTTGAACGGCTGCTTCGAGCAGATCATATGTTTCGAGACGATGAACCCGAAGCTAGCCGAGTCGACTCGGCCGGACGAGTTCCCGGTGGTTCTGAAGCCGTCCATGGAAGCCATGAGGATTGCCATCGAAGCTGCCGAGGTTGATCCTCGTCGAACGCTTTTTCTCGATGACAATGTCCGAAACATCACCGCCGGGAGAGCAGTGGGTCTACGCACCGTTCTGGTTGGAAAAACCGTGAAGAGCAAGGAAGCAGATTATGTGTTAGAGAACGTGAAGAACTTGGCGCAATCAATCTCGGAAGTGTGGGTAGGCGGATCGGAGGGCAGTAATCAACAAGTGAATGGCACCAGAAGCGATATCGAGGTGGATTCCATTCGGACAACCACCGCGGTTGTAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase Hydrolase_like

Protein Analysis

269

Amino Acids

29.77

Weight (kDa)

5.51

Isoelectric Point (pI)

39.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 16 - 204 4.1e-13 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 19 - 210 1.1e-07 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32150 AT2G32150 AT2G32150
fragaria_vesca FvH4_4g15270 FvH4_4g15270
malus_domestica MD13G1191400.v1.1 MD16G1191500.v1.1
prunus_persica Prupe.1G011600_v2.0.a1
pyrus_communis pycom13g16420 pycom16g16110
rosa_chinensis RchiOBHm_Chr4g0416551
rosa_laevigata RLG00000007974
rosa_multiflora Rmu_sc0003722.1_g000037
rosa_roxburghii Rroxscaffold_5G00360440
rosa_rugosa Rorug04G0142800
rosa_samantha Rh4AG205300 Rh4BG202900 Rh4CG216200 Rh4DG202400
rosa_wichuraiana Rw4G017380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 749
AccI GTMKAC 2 cut(s) 467, 613
AccII CGCG 2 cut(s) 380, 794
AciI CCGC 6 cut(s) 147, 248, 594, 717, 792, 794
AclWI GGATC 3 cut(s) 402, 542, 727
AcoI YGGCCR 1 cut(s) 473
AcuI CTGAAG 1 cut(s) 518
AfaI GTAC 2 cut(s) 80, 200
AflIII ACRYGT 2 cut(s) 378, 386
AgsI TTSAA 5 cut(s) 87, 140, 171, 193, 409
AluBI AGCT 8 cut(s) 107, 167, 187, 207, 313, 457, 536, 803
AluI AGCT 8 cut(s) 107, 167, 187, 207, 313, 457, 536, 803
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 1 cut(s) 434
Alw44I GTGCAC 1 cut(s) 269
AlwI GGATC 3 cut(s) 402, 542, 727
AoxI GGCC 1 cut(s) 473
ApaLI GTGCAC 1 cut(s) 269
ApeKI GCWGC 4 cut(s) 204, 330, 414, 536
AspLEI GCGC 1 cut(s) 691
AsuC2I CCSGG 3 cut(s) 155, 488, 598
AsuHPI GGTGA 2 cut(s) 113, 583
AsuNHI GCTAGC 1 cut(s) 457
BaeGI GKGCMC 1 cut(s) 273
BanI GGYRCC 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 4 cut(s) 216, 342, 401, 523
BccI CCATC 1 cut(s) 536
BceAI ACGGC 3 cut(s) 217, 427, 487
BcgI CGANNNNNNTGC 6 cut(s) 79, 113, 193, 227, 227, 261
BcnI CCSGG 3 cut(s) 155, 488, 598
BcoDI GTCTC 1 cut(s) 434
BfaI CTAG 1 cut(s) 458
BisI GCNGC 4 cut(s) 205, 331, 415, 537
BlsI GCNGC 4 cut(s) 206, 332, 416, 538
Bme1390I CCNGG 3 cut(s) 155, 488, 598
BmiI GGNNCC 4 cut(s) 152, 320, 374, 751
BmrFI CCNGG 3 cut(s) 155, 488, 598
BmsI GCATC 3 cut(s) 99, 235, 342
BmtI GCTAGC 1 cut(s) 461
BpuEI CTTGAG 1 cut(s) 95
BpuMI CCSGG 3 cut(s) 155, 488, 598
Bsa29I ATCGAT 1 cut(s) 123
BsaBI GATNNNNATC 1 cut(s) 527
BsaJI CCNNGG 3 cut(s) 507, 540, 792
BsaXI ACNNNNNCTCC 2 cut(s) 191, 221
Bse8I GATNNNNATC 1 cut(s) 527
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 3 cut(s) 507, 540, 792
BseGI GGATG 1 cut(s) 333
BseJI GATNNNNATC 1 cut(s) 527
BseRI GAGGAG 1 cut(s) 25
BseSI GKGCMC 1 cut(s) 273
BseX3I CGGCCG 1 cut(s) 473
BseXI GCAGC 4 cut(s) 216, 342, 401, 523
Bsh1236I CGCG 2 cut(s) 380, 794
Bsh1285I CGRYCG 1 cut(s) 476
BshFI GGCC 1 cut(s) 475
BshNI GGYRCC 1 cut(s) 749
BshVI ATCGAT 1 cut(s) 123
BsiEI CGRYCG 1 cut(s) 476
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 5 cut(s) 155, 216, 476, 488, 597
BsmAI GTCTC 1 cut(s) 434
BsmBI CGTCTC 1 cut(s) 434
BsnI GGCC 1 cut(s) 475
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 6 cut(s) 289, 304, 394, 426, 547, 719
Bsp19I CCATGG 1 cut(s) 507
BspACI CCGC 6 cut(s) 147, 248, 594, 717, 792, 794
BspANI GGCC 1 cut(s) 475
BspDI ATCGAT 1 cut(s) 123
BspFNI CGCG 2 cut(s) 380, 794
BspLI GGNNCC 4 cut(s) 152, 320, 374, 751
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 3 cut(s) 402, 542, 727
BspQI GCTCTTC 1 cut(s) 638
BspT107I GGYRCC 1 cut(s) 749
BssECI CCNNGG 3 cut(s) 507, 540, 792
BssMI GATC 6 cut(s) 289, 304, 394, 426, 547, 719
BssT1I CCWWGG 1 cut(s) 507
Bst4CI ACNGT 3 cut(s) 263, 622, 640
Bst6I CTCTTC 2 cut(s) 88, 638
BstC8I GCNNGC 1 cut(s) 459
BstDSI CCRYGG 2 cut(s) 507, 792
BstF5I GGATG 1 cut(s) 333
BstFNI CGCG 2 cut(s) 380, 794
BstHHI GCGC 1 cut(s) 691
BstKTI GATC 6 cut(s) 292, 307, 397, 429, 550, 722
BstMAI GTCTC 1 cut(s) 434
BstMBI GATC 6 cut(s) 289, 304, 394, 426, 547, 719
BstMCI CGRYCG 1 cut(s) 476
BstMWI GCNNNNNNNGC 4 cut(s) 533, 602, 756, 800
BstNSI RCATGY 1 cut(s) 390
BstSCI CCNGG 3 cut(s) 153, 486, 596
BstSLI GKGCMC 1 cut(s) 273
BstUI CGCG 2 cut(s) 380, 794
BstV1I GCAGC 4 cut(s) 216, 342, 401, 523
BstZI CGGCCG 1 cut(s) 473
Bsu15I ATCGAT 1 cut(s) 123
BsuRI GGCC 1 cut(s) 475
BsuTUI ATCGAT 1 cut(s) 123
BtgI CCRYGG 2 cut(s) 507, 792
BtsCI GGATG 1 cut(s) 333
BtsI GCAGTG 1 cut(s) 612
BtsIMutI CAGTG 1 cut(s) 612
Cac8I GCNNGC 1 cut(s) 459
CfoI GCGC 1 cut(s) 691
Cfr42I CCGCGG 1 cut(s) 795
ClaI ATCGAT 1 cut(s) 123
Csp6I GTAC 2 cut(s) 79, 199
CspCI CAANNNNNGTGG 1 cut(s) 778
CviAII CATG 3 cut(s) 387, 508, 517
CviQI GTAC 2 cut(s) 79, 199
DpnI GATC 6 cut(s) 291, 306, 396, 428, 549, 721
DpnII GATC 6 cut(s) 289, 304, 394, 426, 547, 719
EaeI YGGCCR 1 cut(s) 473
EagI CGGCCG 1 cut(s) 473
Eam1104I CTCTTC 2 cut(s) 88, 638
EarI CTCTTC 2 cut(s) 88, 638
EciI GGCGGA 1 cut(s) 732
EclXI CGGCCG 1 cut(s) 473
Eco130I CCWWGG 1 cut(s) 507
Eco32I GATATC 1 cut(s) 763
Eco52I CGGCCG 1 cut(s) 473
Eco57I CTGAAG 1 cut(s) 518
EcoRV GATATC 1 cut(s) 763
EcoT14I CCWWGG 1 cut(s) 507
ErhI CCWWGG 1 cut(s) 507
Esp3I CGTCTC 1 cut(s) 434
FaeI CATG 3 cut(s) 390, 511, 520
FaiI YATR 7 cut(s) 288, 388, 431, 433, 509, 518, 663
FalI AAGNNNNNCTT 2 cut(s) 268, 300
FatI CATG 3 cut(s) 386, 507, 516
FauI CCCGC 1 cut(s) 140
FauNDI CATATG 1 cut(s) 431
FblI GTMKAC 2 cut(s) 467, 613
Fnu4HI GCNGC 4 cut(s) 205, 331, 415, 537
FokI GGATG 1 cut(s) 320
Fsp4HI GCNGC 4 cut(s) 205, 331, 415, 537
FspBI CTAG 1 cut(s) 458
GlaI GCGC 1 cut(s) 690
GluI GCNGC 4 cut(s) 205, 331, 415, 537
HaeIII GGCC 1 cut(s) 475
HapII CCGG 5 cut(s) 155, 216, 476, 488, 597
HhaI GCGC 1 cut(s) 691
Hin1II CATG 3 cut(s) 390, 511, 520
Hin6I GCGC 1 cut(s) 689
HinP1I GCGC 1 cut(s) 689
HincII GTYRAC 2 cut(s) 384, 468
HindII GTYRAC 2 cut(s) 384, 468
HindIII AAGCTT 1 cut(s) 165
HinfI GANTC 6 cut(s) 5, 96, 294, 464, 469, 773
HpaII CCGG 5 cut(s) 155, 216, 476, 488, 597
HphI GGTGA 2 cut(s) 113, 583
Hpy166II GTNNAC 4 cut(s) 271, 384, 468, 614
Hpy188I TCNGA 7 cut(s) 95, 367, 498, 584, 703, 724, 783
Hpy188III TCNNGA 3 cut(s) 112, 439, 569
Hpy8I GTNNAC 4 cut(s) 271, 384, 468, 614
Hpy99I CGWCG 1 cut(s) 558
HpyAV CCTTC 1 cut(s) 270
HpyCH4III ACNGT 3 cut(s) 263, 622, 640
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 2 cut(s) 12, 271
HpyF10VI GCNNNNNNNGC 4 cut(s) 533, 602, 756, 800
HpySE526I ACGT 1 cut(s) 675
Hsp92II CATG 3 cut(s) 390, 511, 520
HspAI GCGC 1 cut(s) 689
KspI CCGCGG 1 cut(s) 795
Kzo9I GATC 6 cut(s) 289, 304, 394, 426, 547, 719
LguI GCTCTTC 1 cut(s) 638
LmnI GCTCC 1 cut(s) 212
Lsp1109I GCAGC 4 cut(s) 216, 342, 401, 523
LweI GCATC 3 cut(s) 99, 235, 342
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 675
MalI GATC 6 cut(s) 291, 306, 396, 428, 549, 721
MboI GATC 6 cut(s) 289, 304, 394, 426, 547, 719
MboII GAAGA 6 cut(s) 14, 49, 75, 127, 655, 691
MhlI GDGCHC 1 cut(s) 273
MluI ACGCGT 1 cut(s) 378
MlyI GAGTC 3 cut(s) 90, 463, 473
MmeI TCCRAC 1 cut(s) 610
MseI TTAA 1 cut(s) 805
MspA1I CMGCKG 1 cut(s) 794
MspI CCGG 5 cut(s) 155, 216, 476, 488, 597
MspR9I CCNGG 3 cut(s) 155, 488, 598
MvnI CGCG 2 cut(s) 380, 794
MwoI GCNNNNNNNGC 4 cut(s) 533, 602, 756, 800
NciI CCSGG 3 cut(s) 155, 488, 598
NcoI CCATGG 1 cut(s) 507
NdeI CATATG 1 cut(s) 431
NdeII GATC 6 cut(s) 289, 304, 394, 426, 547, 719
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 3 cut(s) 390, 511, 520
NlaIV GGNNCC 4 cut(s) 152, 320, 374, 751
NmeAIII GCCGAG 3 cut(s) 451, 487, 565
NspI RCATGY 1 cut(s) 390
PciI ACATGT 1 cut(s) 386
PciSI GCTCTTC 1 cut(s) 638
PcsI WCGNNNNNNNCGW 3 cut(s) 417, 449, 537
PfeI GAWTC 3 cut(s) 5, 294, 773
PflFI GACNNNGTC 1 cut(s) 578
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 4 cut(s) 206, 332, 416, 538
PleI GAGTC 3 cut(s) 90, 463, 472
PpsI GAGTC 3 cut(s) 90, 463, 472
PscI ACATGT 1 cut(s) 386
PspN4I GGNNCC 4 cut(s) 152, 320, 374, 751
PsyI GACNNNGTC 1 cut(s) 578
RsaI GTAC 2 cut(s) 80, 200
RsaNI GTAC 2 cut(s) 79, 199
SacII CCGCGG 1 cut(s) 795
SalI GTCGAC 1 cut(s) 466
SapI GCTCTTC 1 cut(s) 638
SaqAI TTAA 1 cut(s) 805
SatI GCNGC 4 cut(s) 205, 331, 415, 537
Sau3AI GATC 6 cut(s) 289, 304, 394, 426, 547, 719
SchI GAGTC 3 cut(s) 90, 463, 473
ScrFI CCNGG 3 cut(s) 155, 488, 598
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 3 cut(s) 99, 235, 342
Sfr303I CCGCGG 1 cut(s) 795
SgrBI CCGCGG 1 cut(s) 795
SmlI CTYRAG 1 cut(s) 110
SmoI CTYRAG 1 cut(s) 110
SsiI CCGC 6 cut(s) 147, 248, 594, 717, 792, 794
SspMI CTAG 1 cut(s) 458
StyD4I CCNGG 3 cut(s) 153, 486, 596
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 3 cut(s) 263, 622, 640
TaiI ACGT 1 cut(s) 678
TatI WGTACW 1 cut(s) 78
TfiI GAWTC 3 cut(s) 5, 294, 773
Tru1I TTAA 1 cut(s) 805
Tru9I TTAA 1 cut(s) 805
TscAI CASTG 1 cut(s) 612
TseI GCWGC 4 cut(s) 204, 330, 414, 536
TspDTI ATGAA 1 cut(s) 461
TspGWI ACGGA 2 cut(s) 288, 331
TspRI CASTG 1 cut(s) 612
Tth111I GACNNNGTC 1 cut(s) 578
VneI GTGCAC 1 cut(s) 269
XceI RCATGY 1 cut(s) 390
XmiI GTMKAC 2 cut(s) 467, 613
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.