pycom13g16420

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0010926
Physical Location & Seq
Reverse (-)
12193865 .. 12196031
2167 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16420.7

Sequence Viewer

Length: 807 bp
ATGGATTCCCTCAGTTCCTCTTCTCCTTTTGATTCTATCATTTTCGATTTGGACGACACTCTGTACTCTTCCAACCTTGGACTCGGTGAAGCTTGCAAGAAGAACATCGATGATTTTCTCGTTGAAAAATGTGGATTCCCGGTGAGCAAGGCCTCCAGCCTCCGCGTTGAGCTTTTCAAAAAATACGGCAGCTCCCTCGCTGGCTTACGAGCGTTAGGATACGACATCGACGCCGACGATTACCACGGTGTCGTGCACGGAAGGCTGCCGTACGATCGGATCAAACCCGACCCTCAGCTCCGAAACCTGCTCCGCAGCATCGCCCAGAGAAAAATTATATTTACTAATTCCGACCGGAAGCACGCAGTGAAGGTGTTGGAACGTCTGGGAGTGGTGGAGTGCTTCGATCAGATCATATGTTTCGAGACGATGAACCCGAACCTGCCGAGTTCGACGCGGCCAGACGAGTTCCCGGTGGTCCTGAAGCCGTCCATAGAGGCCATGGAGATCGCACTTCGGGCCGCGGATGTCGATCCTCGCCGCACGCTGTTTCTCGATGACAATGTGCGTAACGTCGCTGCGGGAAAGACTGTTGGTCTTCGCACCGTTTTGGTTGGAAAGACCTTGAAAAGCAAGGAAGCAGATTACTTATTAGAGAACGTGAACAACATGGCACAAGTAATAACGGAGGTATGGGTAGGAGAAGGTGGCGGAAAGGATGGTACTGGTAGTAACCAACGGATCAGCCGCACCAGAAGCGACATCGATCGCGATTCTGTCCTCACTGCCACAGCTGTCGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase Hydrolase_like

Protein Analysis

269

Amino Acids

29.48

Weight (kDa)

5.74

Isoelectric Point (pI)

37.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32150 AT2G32150 AT2G32150
fragaria_vesca FvH4_4g15270 FvH4_4g15270
malus_domestica MD13G1191400.v1.1 MD16G1191500.v1.1
prunus_persica Prupe.1G011600_v2.0.a1
pyrus_communis pycom13g16420 pycom16g16110
rosa_chinensis RchiOBHm_Chr4g0416551
rosa_laevigata RLG00000007974
rosa_multiflora Rmu_sc0003722.1_g000037
rosa_roxburghii Rroxscaffold_5G00360440
rosa_rugosa Rorug04G0142800
rosa_samantha Rh4AG205300 Rh4BG202900 Rh4CG216200 Rh4DG202400
rosa_wichuraiana Rw4G017380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 315, 450
AccII CGCG 4 cut(s) 165, 457, 524, 771
AciI CCGC 9 cut(s) 163, 313, 457, 522, 524, 541, 581, 711, 748
AclWI GGATC 3 cut(s) 287, 527, 749
AcoI YGGCCR 1 cut(s) 458
AcuI CTGAAG 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 231
AdeI CACNNNGTG 1 cut(s) 367
AfaI GTAC 3 cut(s) 65, 272, 724
AgsI TTSAA 3 cut(s) 125, 178, 628
AhdI GACNNNNNGTC 1 cut(s) 594
AluBI AGCT 6 cut(s) 92, 172, 192, 298, 794, 803
AluI AGCT 6 cut(s) 92, 172, 192, 298, 794, 803
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 419
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 3 cut(s) 287, 527, 749
AoxI GGCC 4 cut(s) 150, 458, 498, 519
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 4 cut(s) 189, 265, 315, 578
AspS9I GGNCC 2 cut(s) 478, 519
AsuC2I CCSGG 2 cut(s) 140, 473
AsuHPI GGTGA 2 cut(s) 98, 154
AvaII GGWCC 1 cut(s) 478
BaeGI GKGCMC 1 cut(s) 258
BbsI GAAGAC 1 cut(s) 590
Bbv12I GWGCWC 1 cut(s) 258
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 4 cut(s) 201, 252, 327, 565
BccI CCATC 1 cut(s) 713
BceAI ACGGC 3 cut(s) 202, 253, 472
BcgI CGANNNNNNTGC 2 cut(s) 178, 212
BciVI GTATCC 1 cut(s) 212
BcnI CCSGG 2 cut(s) 140, 473
BcoDI GTCTC 1 cut(s) 419
BfuAI ACCTGC 2 cut(s) 315, 450
BfuI GTATCC 1 cut(s) 212
BisI GCNGC 8 cut(s) 190, 266, 316, 458, 522, 541, 579, 748
BlsI GCNGC 8 cut(s) 191, 267, 317, 459, 523, 542, 580, 749
Bme1390I CCNGG 2 cut(s) 140, 473
Bme18I GGWCC 1 cut(s) 478
BmeRI GACNNNNNGTC 1 cut(s) 594
BmgT120I GGNCC 2 cut(s) 478, 519
BmrFI CCNGG 2 cut(s) 140, 473
BmsI GCATC 1 cut(s) 327
BpiI GAAGAC 1 cut(s) 590
BpmI CTGGAG 1 cut(s) 139
Bpu10I CCTNAGC 1 cut(s) 294
BpuMI CCSGG 2 cut(s) 140, 473
Bsa29I ATCGAT 2 cut(s) 108, 765
BsaBI GATNNNNATC 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 4 cut(s) 76, 244, 501, 522
BsaWI WCCGGW 1 cut(s) 354
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bse1I ACTGG 1 cut(s) 730
Bse8I GATNNNNATC 1 cut(s) 531
BseCI ATCGAT 2 cut(s) 108, 765
BseDI CCNNGG 4 cut(s) 76, 244, 501, 522
BseGI GGATG 2 cut(s) 532, 724
BseJI GATNNNNATC 1 cut(s) 531
BseMII CTCAG 2 cut(s) 25, 308
BseNI ACTGG 1 cut(s) 730
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 4 cut(s) 201, 252, 327, 565
Bsh1236I CGCG 4 cut(s) 165, 457, 524, 771
Bsh1285I CGRYCG 3 cut(s) 277, 355, 769
BshFI GGCC 4 cut(s) 152, 460, 500, 521
BshVI ATCGAT 2 cut(s) 108, 765
BsiEI CGRYCG 3 cut(s) 277, 355, 769
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 3 cut(s) 140, 355, 473
BsiWI CGTACG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 419
BsmBI CGTCTC 1 cut(s) 419
BsnI GGCC 4 cut(s) 152, 460, 500, 521
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 741, 766
Bsp19I CCATGG 1 cut(s) 501
Bsp68I TCGCGA 1 cut(s) 771
BspACI CCGC 9 cut(s) 163, 313, 457, 522, 524, 541, 581, 711, 748
BspANI GGCC 4 cut(s) 152, 460, 500, 521
BspCNI CTCAG 2 cut(s) 24, 307
BspDI ATCGAT 2 cut(s) 108, 765
BspFNI CGCG 4 cut(s) 165, 457, 524, 771
BspMI ACCTGC 2 cut(s) 315, 450
BspPI GGATC 3 cut(s) 287, 527, 749
BsrI ACTGG 1 cut(s) 730
BssECI CCNNGG 4 cut(s) 76, 244, 501, 522
BssMI GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 741, 766
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 2 cut(s) 76, 501
Bst4CI ACNGT 3 cut(s) 248, 592, 607
Bst6I CTCTTC 2 cut(s) 25, 73
BstACI GRCGYC 1 cut(s) 231
BstC8I GCNNGC 4 cut(s) 94, 202, 363, 545
BstDEI CTNAG 2 cut(s) 11, 294
BstDSI CCRYGG 3 cut(s) 244, 501, 522
BstF5I GGATG 2 cut(s) 532, 724
BstFNI CGCG 4 cut(s) 165, 457, 524, 771
BstKTI GATC 8 cut(s) 277, 282, 409, 414, 510, 535, 744, 769
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 741, 766
BstMCI CGRYCG 3 cut(s) 277, 355, 769
BstMWI GCNNNNNNNGC 4 cut(s) 262, 518, 756, 800
BstSCI CCNGG 2 cut(s) 138, 471
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 4 cut(s) 165, 457, 524, 771
BstV1I GCAGC 4 cut(s) 201, 252, 327, 565
BstV2I GAAGAC 1 cut(s) 590
Bsu15I ATCGAT 2 cut(s) 108, 765
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 4 cut(s) 152, 460, 500, 521
BsuTUI ATCGAT 2 cut(s) 108, 765
BtgI CCRYGG 3 cut(s) 244, 501, 522
BtgZI GCGATG 1 cut(s) 304
BtsCI GGATG 2 cut(s) 532, 724
BtsI GCAGTG 2 cut(s) 372, 783
BtsIMutI CAGTG 2 cut(s) 372, 783
BtuMI TCGCGA 1 cut(s) 771
BveI ACCTGC 2 cut(s) 315, 450
Cac8I GCNNGC 4 cut(s) 94, 202, 363, 545
Cfr13I GGNCC 2 cut(s) 478, 519
Cfr42I CCGCGG 1 cut(s) 525
ClaI ATCGAT 2 cut(s) 108, 765
CseI GACGC 2 cut(s) 239, 463
Csp6I GTAC 3 cut(s) 64, 271, 723
CviAII CATG 2 cut(s) 502, 670
CviQI GTAC 3 cut(s) 64, 271, 723
DdeI CTNAG 2 cut(s) 11, 294
DpnI GATC 8 cut(s) 276, 281, 408, 413, 509, 534, 743, 768
DpnII GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 741, 766
DraIII CACNNNGTG 1 cut(s) 367
DriI GACNNNNNGTC 1 cut(s) 594
EaeI YGGCCR 1 cut(s) 458
Eam1104I CTCTTC 2 cut(s) 25, 73
Eam1105I GACNNNNNGTC 1 cut(s) 594
EarI CTCTTC 2 cut(s) 25, 73
EciI GGCGGA 1 cut(s) 726
Eco130I CCWWGG 2 cut(s) 76, 501
Eco147I AGGCCT 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 503
EcoT14I CCWWGG 2 cut(s) 76, 501
ErhI CCWWGG 2 cut(s) 76, 501
Esp3I CGTCTC 1 cut(s) 419
FaeI CATG 2 cut(s) 505, 673
FaiI YATR 7 cut(s) 338, 416, 418, 494, 503, 671, 694
FatI CATG 2 cut(s) 501, 669
FauI CCCGC 1 cut(s) 574
FauNDI CATATG 1 cut(s) 416
Fnu4HI GCNGC 8 cut(s) 190, 266, 316, 458, 522, 541, 579, 748
FokI GGATG 2 cut(s) 539, 731
Fsp4HI GCNGC 8 cut(s) 190, 266, 316, 458, 522, 541, 579, 748
GluI GCNGC 8 cut(s) 190, 266, 316, 458, 522, 541, 579, 748
GsuI CTGGAG 1 cut(s) 139
HaeIII GGCC 4 cut(s) 152, 460, 500, 521
HapII CCGG 3 cut(s) 140, 355, 473
HgaI GACGC 2 cut(s) 239, 463
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 2 cut(s) 505, 673
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 5 cut(s) 5, 32, 81, 135, 773
HpaII CCGG 3 cut(s) 140, 355, 473
HphI GGTGA 2 cut(s) 98, 154
Hpy166II GTNNAC 2 cut(s) 256, 664
Hpy188I TCNGA 5 cut(s) 279, 302, 352, 411, 800
Hpy188III TCNNGA 4 cut(s) 424, 481, 554, 770
Hpy8I GTNNAC 2 cut(s) 256, 664
Hpy99I CGWCG 4 cut(s) 233, 239, 457, 578
HpyAV CCTTC 3 cut(s) 255, 364, 698
HpyCH4III ACNGT 3 cut(s) 248, 592, 607
HpyCH4IV ACGT 3 cut(s) 382, 573, 660
HpyCH4V TGCA 2 cut(s) 96, 256
HpyF10VI GCNNNNNNNGC 4 cut(s) 262, 518, 756, 800
HpyF3I CTNAG 2 cut(s) 11, 294
HpySE526I ACGT 3 cut(s) 382, 573, 660
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 2 cut(s) 505, 673
KspI CCGCGG 1 cut(s) 525
Kzo9I GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 741, 766
LmnI GCTCC 4 cut(s) 197, 303, 315, 800
Lsp1109I GCAGC 4 cut(s) 201, 252, 327, 565
LweI GCATC 1 cut(s) 327
MaeII ACGT 3 cut(s) 382, 573, 660
MaeIII GTNAC 2 cut(s) 569, 731
MalI GATC 8 cut(s) 276, 281, 408, 413, 509, 534, 743, 768
MboI GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 741, 766
MboII GAAGA 4 cut(s) 12, 60, 112, 590
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 2 cut(s) 333, 346
MlyI GAGTC 1 cut(s) 75
MmeI TCCRAC 5 cut(s) 96, 357, 375, 595, 778
MspA1I CMGCKG 2 cut(s) 524, 794
MspI CCGG 3 cut(s) 140, 355, 473
MspR9I CCNGG 2 cut(s) 140, 473
MvnI CGCG 4 cut(s) 165, 457, 524, 771
MwoI GCNNNNNNNGC 4 cut(s) 262, 518, 756, 800
NciI CCSGG 2 cut(s) 140, 473
NcoI CCATGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 416
NdeII GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 741, 766
NlaIII CATG 2 cut(s) 505, 673
NmeAIII GCCGAG 1 cut(s) 471
NruI TCGCGA 1 cut(s) 771
PceI AGGCCT 1 cut(s) 152
PcsI WCGNNNNNNNCGW 3 cut(s) 51, 234, 434
PfeI GAWTC 4 cut(s) 5, 32, 135, 773
Pfl23II CGTACG 1 cut(s) 270
PkrI GCNGC 8 cut(s) 191, 267, 317, 459, 523, 542, 580, 749
Ple19I CGATCG 2 cut(s) 277, 769
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
PspLI CGTACG 1 cut(s) 270
PspPI GGNCC 2 cut(s) 478, 519
PvuI CGATCG 2 cut(s) 277, 769
PvuII CAGCTG 1 cut(s) 794
RruI TCGCGA 1 cut(s) 771
RsaI GTAC 3 cut(s) 65, 272, 724
RsaNI GTAC 3 cut(s) 64, 271, 723
SacII CCGCGG 1 cut(s) 525
SatI GCNGC 8 cut(s) 190, 266, 316, 458, 522, 541, 579, 748
Sau3AI GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 741, 766
Sau96I GGNCC 2 cut(s) 478, 519
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 2 cut(s) 140, 473
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 1 cut(s) 327
Sfr303I CCGCGG 1 cut(s) 525
SgrBI CCGCGG 1 cut(s) 525
SinI GGWCC 1 cut(s) 478
Sse9I AATT 2 cut(s) 333, 346
SseBI AGGCCT 1 cut(s) 152
SsiI CCGC 9 cut(s) 163, 313, 457, 522, 524, 541, 581, 711, 748
StuI AGGCCT 1 cut(s) 152
StyD4I CCNGG 2 cut(s) 138, 471
StyI CCWWGG 2 cut(s) 76, 501
TaaI ACNGT 3 cut(s) 248, 592, 607
TaiI ACGT 3 cut(s) 385, 576, 663
TaqI TCGA 9 cut(s) 45, 108, 228, 405, 423, 452, 531, 555, 765
TasI AATT 2 cut(s) 333, 346
TatI WGTACW 1 cut(s) 63
TauI GCSGC 4 cut(s) 460, 524, 543, 750
TfiI GAWTC 4 cut(s) 5, 32, 135, 773
TscAI CASTG 2 cut(s) 372, 790
TseI GCWGC 4 cut(s) 189, 265, 315, 578
TspDTI ATGAA 1 cut(s) 446
TspGWI ACGGA 3 cut(s) 273, 701, 754
TspRI CASTG 2 cut(s) 372, 790
VneI GTGCAC 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.