MD13G1191400.v1.1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010926
Physical Location & Seq
Reverse (-)
16398050 .. 16401705
3656 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1191400.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGATTCCCTCAGTTCCTCTTCTCCCCTTGATTCTATCATCTTCGATTTGGACGACACTCTGTACCCTTCCAACCTTGGACTCGGTGAAGCTTGCAAGAAGAACATCGATGATTTTCTCGTTGAAAAATGTGGATTCCCGGTGAGCAAGGCCTCCAGCCTCCGCGTTGAGCTTTTCAAAAAATACGGCAGTTCCCTCGCTGGCTTACGAGCGTTAGGATACGACATCGACGCCGACGATTACCACGGTGTCGTGCACGGGAGGCTGCCGTACGATCGGATCAAACCCGACCCTCAGCTCCGAAACCTGCTCCGCAGCATCGCCCAGAGAAAAATTATATTTACTAATTCCGACAGGAAGCACGCAGTGAAGGTGTTGGAACGTCTGGGAGTGGTGGAGTGCTTCGATCAGATCATATGTTTCGAGACGATGAACCCGAACCTGCCGAGTTCGACGAGGCCGGACGAGTTCCCGGTGGTCCTGAAGCCGTCCATAGAGGCCATGGAGATCGCGCTTCGGGCCGCGGATGTCGATCCTCGCCGCACGCTGTTTCTCGATGACAACGTGCGTAACGTCGCTGCGGGAAAGGCTGTTGGTTTTCGCACCGTTTTGGTTGGAATGACCGTGAAAAGCAAGGAAGCAGATTACTTATTAGAGAACATGAACAACATGGCACAAGTAATAACGGAAGTATGGGTAGGAGGAGGTGGCGGAAAGGATGGTAGTAACCAACGGATCAGCCGCACCAGGAGCGAAATCGATCGCGATTCCGTCCTCACCGCCACAGCTGTCGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase Hydrolase_like

Protein Analysis

267

Amino Acids

29.27

Weight (kDa)

5.73

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 11 - 199 2.8e-15 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 13 - 205 1.1e-08 Haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32150 AT2G32150 AT2G32150
fragaria_vesca FvH4_4g15270 FvH4_4g15270
malus_domestica MD13G1191400.v1.1 MD16G1191500.v1.1
prunus_persica Prupe.1G011600_v2.0.a1
pyrus_communis pycom13g16420 pycom16g16110
rosa_chinensis RchiOBHm_Chr4g0416551
rosa_laevigata RLG00000007974
rosa_multiflora Rmu_sc0003722.1_g000037
rosa_roxburghii Rroxscaffold_5G00360440
rosa_rugosa Rorug04G0142800
rosa_samantha Rh4AG205300 Rh4BG202900 Rh4CG216200 Rh4DG202400
rosa_wichuraiana Rw4G017380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 315, 450
AccII CGCG 4 cut(s) 165, 512, 524, 765
AciI CCGC 9 cut(s) 163, 313, 522, 524, 541, 581, 711, 742, 780
AclWI GGATC 3 cut(s) 287, 527, 743
AcuI CTGAAG 1 cut(s) 503
AcyI GRCGYC 1 cut(s) 231
AdeI CACNNNGTG 1 cut(s) 367
AfaI GTAC 2 cut(s) 65, 272
AgsI TTSAA 2 cut(s) 125, 178
AjnI CCWGG 1 cut(s) 746
AluBI AGCT 5 cut(s) 92, 172, 298, 788, 797
AluI AGCT 5 cut(s) 92, 172, 298, 788, 797
Alw21I GWGCWC 1 cut(s) 258
Alw26I GTCTC 1 cut(s) 419
Alw44I GTGCAC 1 cut(s) 254
AlwI GGATC 3 cut(s) 287, 527, 743
AoxI GGCC 4 cut(s) 150, 458, 498, 519
ApaLI GTGCAC 1 cut(s) 254
ApeKI GCWGC 3 cut(s) 265, 315, 578
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 2 cut(s) 478, 519
AsuC2I CCSGG 2 cut(s) 140, 473
AsuHPI GGTGA 3 cut(s) 98, 154, 769
AvaII GGWCC 1 cut(s) 478
BaeGI GKGCMC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 258
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 3 cut(s) 252, 327, 565
BccI CCATC 1 cut(s) 713
BceAI ACGGC 3 cut(s) 202, 253, 472
BcgI CGANNNNNNTGC 2 cut(s) 178, 212
BciT130I CCWGG 1 cut(s) 748
BciVI GTATCC 1 cut(s) 212
BcnI CCSGG 2 cut(s) 140, 473
BcoDI GTCTC 1 cut(s) 419
BfuAI ACCTGC 2 cut(s) 315, 450
BfuI GTATCC 1 cut(s) 212
BisI GCNGC 6 cut(s) 266, 316, 522, 541, 579, 742
BlsI GCNGC 6 cut(s) 267, 317, 523, 542, 580, 743
Bme1390I CCNGG 3 cut(s) 140, 473, 748
Bme18I GGWCC 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 478, 519
BmrFI CCNGG 3 cut(s) 140, 473, 748
BmsI GCATC 1 cut(s) 327
BpmI CTGGAG 1 cut(s) 139
Bpu10I CCTNAGC 1 cut(s) 294
BpuMI CCSGG 2 cut(s) 140, 473
Bsa29I ATCGAT 2 cut(s) 108, 759
BsaBI GATNNNNATC 1 cut(s) 531
BsaHI GRCGYC 1 cut(s) 231
BsaJI CCNNGG 4 cut(s) 76, 244, 501, 522
Bse8I GATNNNNATC 1 cut(s) 531
BseBI CCWGG 1 cut(s) 748
BseCI ATCGAT 2 cut(s) 108, 759
BseDI CCNNGG 4 cut(s) 76, 244, 501, 522
BseGI GGATG 2 cut(s) 532, 724
BseJI GATNNNNATC 1 cut(s) 531
BseMII CTCAG 2 cut(s) 25, 308
BseRI GAGGAG 1 cut(s) 717
BseSI GKGCMC 1 cut(s) 258
BseXI GCAGC 3 cut(s) 252, 327, 565
Bsh1236I CGCG 4 cut(s) 165, 512, 524, 765
Bsh1285I CGRYCG 2 cut(s) 277, 763
BshFI GGCC 4 cut(s) 152, 460, 500, 521
BshVI ATCGAT 2 cut(s) 108, 759
BsiEI CGRYCG 2 cut(s) 277, 763
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 3 cut(s) 140, 461, 473
BsiWI CGTACG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 419
BsmBI CGTCTC 1 cut(s) 419
BsnI GGCC 4 cut(s) 152, 460, 500, 521
Bsp1286I GDGCHC 1 cut(s) 258
Bsp143I GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 735, 760
Bsp19I CCATGG 1 cut(s) 501
Bsp68I TCGCGA 1 cut(s) 765
BspACI CCGC 9 cut(s) 163, 313, 522, 524, 541, 581, 711, 742, 780
BspANI GGCC 4 cut(s) 152, 460, 500, 521
BspCNI CTCAG 2 cut(s) 24, 307
BspDI ATCGAT 2 cut(s) 108, 759
BspFNI CGCG 4 cut(s) 165, 512, 524, 765
BspMI ACCTGC 2 cut(s) 315, 450
BspPI GGATC 3 cut(s) 287, 527, 743
BssECI CCNNGG 4 cut(s) 76, 244, 501, 522
BssMI GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 735, 760
BssNI GRCGYC 1 cut(s) 231
BssT1I CCWWGG 2 cut(s) 76, 501
Bst2UI CCWGG 1 cut(s) 748
Bst4CI ACNGT 3 cut(s) 248, 607, 625
Bst6I CTCTTC 1 cut(s) 25
BstACI GRCGYC 1 cut(s) 231
BstC8I GCNNGC 4 cut(s) 94, 202, 363, 545
BstDEI CTNAG 2 cut(s) 11, 294
BstDSI CCRYGG 3 cut(s) 244, 501, 522
BstF5I GGATG 2 cut(s) 532, 724
BstFNI CGCG 4 cut(s) 165, 512, 524, 765
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 8 cut(s) 277, 282, 409, 414, 510, 535, 738, 763
BstMAI GTCTC 1 cut(s) 419
BstMBI GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 735, 760
BstMCI CGRYCG 2 cut(s) 277, 763
BstMWI GCNNNNNNNGC 5 cut(s) 262, 518, 587, 750, 794
BstNI CCWGG 1 cut(s) 748
BstSCI CCNGG 3 cut(s) 138, 471, 746
BstSLI GKGCMC 1 cut(s) 258
BstUI CGCG 4 cut(s) 165, 512, 524, 765
BstV1I GCAGC 3 cut(s) 252, 327, 565
Bsu15I ATCGAT 2 cut(s) 108, 759
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 4 cut(s) 152, 460, 500, 521
BsuTUI ATCGAT 2 cut(s) 108, 759
BtgI CCRYGG 3 cut(s) 244, 501, 522
BtgZI GCGATG 1 cut(s) 304
BtsCI GGATG 2 cut(s) 532, 724
BtsI GCAGTG 1 cut(s) 372
BtsIMutI CAGTG 1 cut(s) 372
BtuMI TCGCGA 1 cut(s) 765
BveI ACCTGC 2 cut(s) 315, 450
Cac8I GCNNGC 4 cut(s) 94, 202, 363, 545
CfoI GCGC 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 478, 519
Cfr42I CCGCGG 1 cut(s) 525
ClaI ATCGAT 2 cut(s) 108, 759
CseI GACGC 1 cut(s) 239
Csp6I GTAC 2 cut(s) 64, 271
CviAII CATG 3 cut(s) 502, 661, 670
CviQI GTAC 2 cut(s) 64, 271
DdeI CTNAG 2 cut(s) 11, 294
DpnI GATC 8 cut(s) 276, 281, 408, 413, 509, 534, 737, 762
DpnII GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 735, 760
DraIII CACNNNGTG 1 cut(s) 367
Eam1104I CTCTTC 1 cut(s) 25
EarI CTCTTC 1 cut(s) 25
EciI GGCGGA 1 cut(s) 726
Eco130I CCWWGG 2 cut(s) 76, 501
Eco147I AGGCCT 1 cut(s) 152
Eco47I GGWCC 1 cut(s) 478
Eco57I CTGAAG 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 746
EcoT14I CCWWGG 2 cut(s) 76, 501
ErhI CCWWGG 2 cut(s) 76, 501
Esp3I CGTCTC 1 cut(s) 419
FaeI CATG 3 cut(s) 505, 664, 673
FaiI YATR 8 cut(s) 338, 416, 418, 494, 503, 662, 671, 694
FatI CATG 3 cut(s) 501, 660, 669
FauI CCCGC 1 cut(s) 574
FauNDI CATATG 1 cut(s) 416
Fnu4HI GCNGC 6 cut(s) 266, 316, 522, 541, 579, 742
FokI GGATG 2 cut(s) 539, 731
Fsp4HI GCNGC 6 cut(s) 266, 316, 522, 541, 579, 742
GlaI GCGC 1 cut(s) 513
GluI GCNGC 6 cut(s) 266, 316, 522, 541, 579, 742
GsuI CTGGAG 1 cut(s) 139
HaeIII GGCC 4 cut(s) 152, 460, 500, 521
HapII CCGG 3 cut(s) 140, 461, 473
HgaI GACGC 1 cut(s) 239
HhaI GCGC 1 cut(s) 514
Hin1I GRCGYC 1 cut(s) 231
Hin1II CATG 3 cut(s) 505, 664, 673
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 5 cut(s) 5, 32, 81, 135, 767
HpaII CCGG 3 cut(s) 140, 461, 473
HphI GGTGA 3 cut(s) 98, 154, 769
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 5 cut(s) 279, 302, 352, 411, 794
Hpy188III TCNNGA 4 cut(s) 424, 481, 554, 764
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 4 cut(s) 233, 239, 457, 578
HpyAV CCTTC 2 cut(s) 78, 364
HpyCH4III ACNGT 3 cut(s) 248, 607, 625
HpyCH4IV ACGT 3 cut(s) 382, 564, 573
HpyCH4V TGCA 2 cut(s) 96, 256
HpyF10VI GCNNNNNNNGC 5 cut(s) 262, 518, 587, 750, 794
HpyF3I CTNAG 2 cut(s) 11, 294
HpySE526I ACGT 3 cut(s) 382, 564, 573
Hsp92I GRCGYC 1 cut(s) 231
Hsp92II CATG 3 cut(s) 505, 664, 673
HspAI GCGC 1 cut(s) 512
KspI CCGCGG 1 cut(s) 525
Kzo9I GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 735, 760
LmnI GCTCC 4 cut(s) 303, 315, 750, 794
Lsp1109I GCAGC 3 cut(s) 252, 327, 565
LweI GCATC 1 cut(s) 327
MaeII ACGT 3 cut(s) 382, 564, 573
MaeIII GTNAC 2 cut(s) 569, 725
MalI GATC 8 cut(s) 276, 281, 408, 413, 509, 534, 737, 762
MboI GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 735, 760
MboII GAAGA 3 cut(s) 12, 34, 112
MhlI GDGCHC 1 cut(s) 258
MluCI AATT 2 cut(s) 333, 346
MlyI GAGTC 1 cut(s) 75
MmeI TCCRAC 5 cut(s) 96, 357, 375, 595, 772
MspA1I CMGCKG 2 cut(s) 524, 788
MspI CCGG 3 cut(s) 140, 461, 473
MspR9I CCNGG 3 cut(s) 140, 473, 748
MvaI CCWGG 1 cut(s) 748
MvnI CGCG 4 cut(s) 165, 512, 524, 765
MwoI GCNNNNNNNGC 5 cut(s) 262, 518, 587, 750, 794
NciI CCSGG 2 cut(s) 140, 473
NcoI CCATGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 416
NdeII GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 735, 760
NlaIII CATG 3 cut(s) 505, 664, 673
NmeAIII GCCGAG 1 cut(s) 471
NruI TCGCGA 1 cut(s) 765
PceI AGGCCT 1 cut(s) 152
PcsI WCGNNNNNNNCGW 5 cut(s) 51, 234, 434, 561, 570
PfeI GAWTC 4 cut(s) 5, 32, 135, 767
Pfl23II CGTACG 1 cut(s) 270
PkrI GCNGC 6 cut(s) 267, 317, 523, 542, 580, 743
Ple19I CGATCG 2 cut(s) 277, 763
PleI GAGTC 1 cut(s) 75
PpsI GAGTC 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 746
PspGI CCWGG 1 cut(s) 746
PspLI CGTACG 1 cut(s) 270
PspPI GGNCC 2 cut(s) 478, 519
PvuI CGATCG 2 cut(s) 277, 763
PvuII CAGCTG 1 cut(s) 788
RruI TCGCGA 1 cut(s) 765
RsaI GTAC 2 cut(s) 65, 272
RsaNI GTAC 2 cut(s) 64, 271
SacII CCGCGG 1 cut(s) 525
SatI GCNGC 6 cut(s) 266, 316, 522, 541, 579, 742
Sau3AI GATC 8 cut(s) 274, 279, 406, 411, 507, 532, 735, 760
Sau96I GGNCC 2 cut(s) 478, 519
SchI GAGTC 1 cut(s) 75
ScrFI CCNGG 3 cut(s) 140, 473, 748
SduI GDGCHC 1 cut(s) 258
SfaNI GCATC 1 cut(s) 327
Sfr303I CCGCGG 1 cut(s) 525
SgrBI CCGCGG 1 cut(s) 525
SinI GGWCC 1 cut(s) 478
Sse9I AATT 2 cut(s) 333, 346
SseBI AGGCCT 1 cut(s) 152
SsiI CCGC 9 cut(s) 163, 313, 522, 524, 541, 581, 711, 742, 780
StuI AGGCCT 1 cut(s) 152
StyD4I CCNGG 3 cut(s) 138, 471, 746
StyI CCWWGG 2 cut(s) 76, 501
TaaI ACNGT 3 cut(s) 248, 607, 625
TaiI ACGT 3 cut(s) 385, 567, 576
TaqI TCGA 9 cut(s) 45, 108, 228, 405, 423, 452, 531, 555, 759
TasI AATT 2 cut(s) 333, 346
TauI GCSGC 3 cut(s) 524, 543, 744
TfiI GAWTC 4 cut(s) 5, 32, 135, 767
TscAI CASTG 1 cut(s) 372
TseI GCWGC 3 cut(s) 265, 315, 578
TspDTI ATGAA 2 cut(s) 446, 677
TspGWI ACGGA 3 cut(s) 701, 748, 760
TspRI CASTG 1 cut(s) 372
VneI GTGCAC 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.