MD16G1191500.v1.1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010926
Physical Location & Seq
Reverse (-)
16911374 .. 16913990
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1191500.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGATTCCCGCAGTTCCTCTTCTCCTTTTGATTCCATCATTTTCGATTTGGATGACACTCTGTACTCTTCCAACCTTGGACTCGGTGAAGCTTGCAAGAAGAACATCGATGATTTTCTTGTTGAAAAATGCGGATTCCCGGAGAGCAAGGCGTCCAGCCTCCGCGTTGAGCTTTTCAAAAAATACGGCAGCTCCCTCGCTGGCTTACGAGCGTTGGGGTACGACATCGACGCCGAAGATTACCACGACGTCGTGCATGGAAGGCTGCCGTACGATCGGATCAAACCCGACCCTCAGCTCCGAGACCTCCTCCGCAGCATCGCGCAAAGAAAAATCATATTTACGAATTCGGACCGGAAGCACGCGATGAAGGTGTTGGAACGTCTGGGAGTGGAGGAGTGCTTCGATCGGATCATATGCTTCGAGACAATGAACCCAAACCTGCAAAGTTCGACTCAGCCGGACGAGTTCCCGGTGGTCCTGAAGCCGTCCATAGAGGCCATGGAGATCGCGCTCCGGGCTGCGGAGGTCGATCCTCGCCGCACGCTGTTTCTCGATGACAACGTGCGTAACGTCGCAGCGGGGAAAGCTGTTGGTCTACGCACCGTTTTGGTTGGAAAAACCGTGAAAAGCAAGGAAGCAGATTACGTGTTAGAGAATGTGAACAACATGGCACAAGCAATATCAGAAGTATGGGTGGGAGGAAGCGGCGCAAAGGATGGTAGCAACCAAAGGATCAGCCGCACCCGAAGCGACATCGAGCTTGATTCCGTCCTCACCGCCACAGCTGTCGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase Hydrolase_like

Protein Analysis

267

Amino Acids

29.42

Weight (kDa)

5.28

Isoelectric Point (pI)

47.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 11 - 199 9.9e-17 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 13 - 205 2e-11 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 162 - 213 4.4e-06 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32150 AT2G32150 AT2G32150
fragaria_vesca FvH4_4g15270 FvH4_4g15270
malus_domestica MD13G1191400.v1.1 MD16G1191500.v1.1
prunus_persica Prupe.1G011600_v2.0.a1
pyrus_communis pycom13g16420 pycom16g16110
rosa_chinensis RchiOBHm_Chr4g0416551
rosa_laevigata RLG00000007974
rosa_multiflora Rmu_sc0003722.1_g000037
rosa_roxburghii Rroxscaffold_5G00360440
rosa_rugosa Rorug04G0142800
rosa_samantha Rh4AG205300 Rh4BG202900 Rh4CG216200 Rh4DG202400
rosa_wichuraiana Rw4G017380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 252
Acc36I ACCTGC 1 cut(s) 450
AccI GTMKAC 1 cut(s) 598
AccII CGCG 4 cut(s) 165, 323, 365, 512
AclWI GGATC 4 cut(s) 287, 419, 527, 743
AcsI RAATTY 1 cut(s) 346
AcuI CTGAAG 1 cut(s) 503
AcyI GRCGYC 3 cut(s) 152, 231, 249
AfaI GTAC 3 cut(s) 65, 221, 272
AfiI CCNNNNNNNGG 1 cut(s) 523
AflIII ACRYGT 1 cut(s) 648
AgsI TTSAA 2 cut(s) 125, 178
AluBI AGCT 8 cut(s) 92, 172, 192, 298, 590, 763, 788, 797
AluI AGCT 8 cut(s) 92, 172, 192, 298, 590, 763, 788, 797
Alw26I GTCTC 2 cut(s) 297, 419
AlwI GGATC 4 cut(s) 287, 419, 527, 743
AoxI GGCC 1 cut(s) 498
ApeKI GCWGC 5 cut(s) 189, 265, 315, 521, 578
ApoI RAATTY 1 cut(s) 346
AspLEI GCGC 3 cut(s) 325, 514, 713
AspS9I GGNCC 2 cut(s) 352, 478
AsuC2I CCSGG 3 cut(s) 140, 473, 518
AsuHPI GGTGA 2 cut(s) 98, 769
AvaII GGWCC 2 cut(s) 352, 478
BbvCI CCTCAGC 1 cut(s) 294
BbvI GCAGC 5 cut(s) 201, 252, 327, 508, 590
BccI CCATC 2 cut(s) 44, 713
BceAI ACGGC 3 cut(s) 202, 253, 472
BcgI CGANNNNNNTGC 2 cut(s) 178, 212
BcnI CCSGG 3 cut(s) 140, 473, 518
BcoDI GTCTC 2 cut(s) 297, 419
BfuAI ACCTGC 1 cut(s) 450
BisI GCNGC 8 cut(s) 190, 266, 316, 522, 541, 579, 709, 742
BlsI GCNGC 8 cut(s) 191, 267, 317, 523, 542, 580, 710, 743
Bme1390I CCNGG 3 cut(s) 140, 473, 518
Bme18I GGWCC 2 cut(s) 352, 478
BmgT120I GGNCC 2 cut(s) 352, 478
BmrFI CCNGG 3 cut(s) 140, 473, 518
BmsI GCATC 1 cut(s) 327
BplI GAGNNNNNCTC 2 cut(s) 294, 326
Bpu10I CCTNAGC 1 cut(s) 294
BpuMI CCSGG 3 cut(s) 140, 473, 518
Bsa29I ATCGAT 1 cut(s) 108
BsaAI YACGTR 1 cut(s) 649
BsaHI GRCGYC 3 cut(s) 152, 231, 249
BsaI GGTCTC 1 cut(s) 297
BsaJI CCNNGG 2 cut(s) 76, 501
BsaWI WCCGGW 1 cut(s) 354
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 523
BseCI ATCGAT 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 76, 501
BseGI GGATG 2 cut(s) 58, 724
BseLI CCNNNNNNNGG 1 cut(s) 523
BseMII CTCAG 2 cut(s) 308, 470
BseRI GAGGAG 2 cut(s) 299, 410
BseXI GCAGC 5 cut(s) 201, 252, 327, 508, 590
Bsh1236I CGCG 4 cut(s) 165, 323, 365, 512
Bsh1285I CGRYCG 2 cut(s) 277, 409
BshFI GGCC 1 cut(s) 500
BshVI ATCGAT 1 cut(s) 108
BsiEI CGRYCG 2 cut(s) 277, 409
BsiSI CCGG 5 cut(s) 140, 355, 461, 473, 517
BsiWI CGTACG 1 cut(s) 270
BslI CCNNNNNNNGG 1 cut(s) 523
BsmAI GTCTC 2 cut(s) 297, 419
BsnI GGCC 1 cut(s) 500
Bso31I GGTCTC 1 cut(s) 297
Bsp143I GATC 7 cut(s) 274, 279, 406, 411, 507, 532, 735
Bsp19I CCATGG 1 cut(s) 501
BspANI GGCC 1 cut(s) 500
BspCNI CTCAG 2 cut(s) 307, 469
BspDI ATCGAT 1 cut(s) 108
BspFNI CGCG 4 cut(s) 165, 323, 365, 512
BspMI ACCTGC 1 cut(s) 450
BspPI GGATC 4 cut(s) 287, 419, 527, 743
BspTNI GGTCTC 1 cut(s) 297
BssECI CCNNGG 2 cut(s) 76, 501
BssMI GATC 7 cut(s) 274, 279, 406, 411, 507, 532, 735
BssNI GRCGYC 3 cut(s) 152, 231, 249
BssT1I CCWWGG 2 cut(s) 76, 501
Bst4CI ACNGT 2 cut(s) 607, 625
Bst6I CTCTTC 2 cut(s) 25, 73
BstACI GRCGYC 3 cut(s) 152, 231, 249
BstBAI YACGTR 1 cut(s) 649
BstC8I GCNNGC 4 cut(s) 94, 202, 363, 545
BstDEI CTNAG 2 cut(s) 294, 456
BstDSI CCRYGG 1 cut(s) 501
BstF5I GGATG 2 cut(s) 58, 724
BstFNI CGCG 4 cut(s) 165, 323, 365, 512
BstHHI GCGC 3 cut(s) 325, 514, 713
BstKTI GATC 7 cut(s) 277, 282, 409, 414, 510, 535, 738
BstMAI GTCTC 2 cut(s) 297, 419
BstMBI GATC 7 cut(s) 274, 279, 406, 411, 507, 532, 735
BstMCI CGRYCG 2 cut(s) 277, 409
BstMWI GCNNNNNNNGC 5 cut(s) 262, 518, 587, 750, 794
BstSCI CCNGG 3 cut(s) 138, 471, 516
BstUI CGCG 4 cut(s) 165, 323, 365, 512
BstV1I GCAGC 5 cut(s) 201, 252, 327, 508, 590
Bsu15I ATCGAT 1 cut(s) 108
BsuRI GGCC 1 cut(s) 500
BsuTUI ATCGAT 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 501
BtgZI GCGATG 2 cut(s) 304, 380
BtsCI GGATG 2 cut(s) 58, 724
BveI ACCTGC 1 cut(s) 450
Cac8I GCNNGC 4 cut(s) 94, 202, 363, 545
CfoI GCGC 3 cut(s) 325, 514, 713
Cfr13I GGNCC 2 cut(s) 352, 478
ClaI ATCGAT 1 cut(s) 108
CpoI CGGWCCG 1 cut(s) 352
CseI GACGC 2 cut(s) 141, 239
Csp6I GTAC 3 cut(s) 64, 220, 271
CspI CGGWCCG 1 cut(s) 352
CviAII CATG 3 cut(s) 257, 502, 670
CviQI GTAC 3 cut(s) 64, 220, 271
DdeI CTNAG 2 cut(s) 294, 456
DpnI GATC 7 cut(s) 276, 281, 408, 413, 509, 534, 737
DpnII GATC 7 cut(s) 274, 279, 406, 411, 507, 532, 735
Eam1104I CTCTTC 2 cut(s) 25, 73
EarI CTCTTC 2 cut(s) 25, 73
Eco130I CCWWGG 2 cut(s) 76, 501
Eco31I GGTCTC 1 cut(s) 297
Eco47I GGWCC 2 cut(s) 352, 478
Eco57I CTGAAG 1 cut(s) 503
EcoRI GAATTC 1 cut(s) 346
EcoT14I CCWWGG 2 cut(s) 76, 501
ErhI CCWWGG 2 cut(s) 76, 501
FaeI CATG 3 cut(s) 260, 505, 673
FaiI YATR 8 cut(s) 258, 338, 416, 418, 494, 503, 671, 694
FatI CATG 3 cut(s) 256, 501, 669
FauI CCCGC 2 cut(s) 17, 574
FauNDI CATATG 1 cut(s) 416
FblI GTMKAC 1 cut(s) 598
Fnu4HI GCNGC 8 cut(s) 190, 266, 316, 522, 541, 579, 709, 742
FokI GGATG 2 cut(s) 65, 731
Fsp4HI GCNGC 8 cut(s) 190, 266, 316, 522, 541, 579, 709, 742
GlaI GCGC 3 cut(s) 324, 513, 712
GluI GCNGC 8 cut(s) 190, 266, 316, 522, 541, 579, 709, 742
HaeIII GGCC 1 cut(s) 500
HapII CCGG 5 cut(s) 140, 355, 461, 473, 517
HgaI GACGC 2 cut(s) 141, 239
HhaI GCGC 3 cut(s) 325, 514, 713
Hin1I GRCGYC 3 cut(s) 152, 231, 249
Hin1II CATG 3 cut(s) 260, 505, 673
Hin6I GCGC 3 cut(s) 323, 512, 711
HinP1I GCGC 3 cut(s) 323, 512, 711
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 6 cut(s) 5, 32, 81, 135, 454, 767
HpaII CCGG 5 cut(s) 140, 355, 461, 473, 517
HphI GGTGA 2 cut(s) 98, 769
Hpy166II GTNNAC 2 cut(s) 599, 664
Hpy188I TCNGA 6 cut(s) 279, 302, 352, 411, 688, 794
Hpy188III TCNNGA 3 cut(s) 424, 481, 554
Hpy8I GTNNAC 2 cut(s) 599, 664
Hpy99I CGWCG 4 cut(s) 233, 251, 254, 578
HpyAV CCTTC 2 cut(s) 255, 364
HpyCH4III ACNGT 2 cut(s) 607, 625
HpyCH4IV ACGT 5 cut(s) 249, 382, 564, 573, 648
HpyCH4V TGCA 3 cut(s) 96, 256, 445
HpyF10VI GCNNNNNNNGC 5 cut(s) 262, 518, 587, 750, 794
HpyF3I CTNAG 2 cut(s) 294, 456
HpySE526I ACGT 5 cut(s) 249, 382, 564, 573, 648
Hsp92I GRCGYC 3 cut(s) 152, 231, 249
Hsp92II CATG 3 cut(s) 260, 505, 673
HspAI GCGC 3 cut(s) 323, 512, 711
Kzo9I GATC 7 cut(s) 274, 279, 406, 411, 507, 532, 735
LmnI GCTCC 4 cut(s) 197, 303, 519, 794
Lsp1109I GCAGC 5 cut(s) 201, 252, 327, 508, 590
LweI GCATC 1 cut(s) 327
MaeII ACGT 5 cut(s) 249, 382, 564, 573, 648
MaeIII GTNAC 1 cut(s) 569
MalI GATC 7 cut(s) 276, 281, 408, 413, 509, 534, 737
MboI GATC 7 cut(s) 274, 279, 406, 411, 507, 532, 735
MboII GAAGA 4 cut(s) 12, 60, 112, 248
MluCI AATT 1 cut(s) 346
MlyI GAGTC 2 cut(s) 75, 448
MmeI TCCRAC 4 cut(s) 96, 357, 595, 772
MspA1I CMGCKG 2 cut(s) 581, 788
MspI CCGG 5 cut(s) 140, 355, 461, 473, 517
MspR9I CCNGG 3 cut(s) 140, 473, 518
MvnI CGCG 4 cut(s) 165, 323, 365, 512
MwoI GCNNNNNNNGC 5 cut(s) 262, 518, 587, 750, 794
NciI CCSGG 3 cut(s) 140, 473, 518
NcoI CCATGG 1 cut(s) 501
NdeI CATATG 1 cut(s) 416
NdeII GATC 7 cut(s) 274, 279, 406, 411, 507, 532, 735
NlaIII CATG 3 cut(s) 260, 505, 673
PcsI WCGNNNNNNNCGW 2 cut(s) 561, 570
PfeI GAWTC 4 cut(s) 5, 32, 135, 767
Pfl23II CGTACG 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 8 cut(s) 191, 267, 317, 523, 542, 580, 710, 743
Ple19I CGATCG 2 cut(s) 277, 409
PleI GAGTC 2 cut(s) 75, 448
PpsI GAGTC 2 cut(s) 75, 448
Ppu21I YACGTR 1 cut(s) 649
PspLI CGTACG 1 cut(s) 270
PspPI GGNCC 2 cut(s) 352, 478
PvuI CGATCG 2 cut(s) 277, 409
PvuII CAGCTG 1 cut(s) 788
RsaI GTAC 3 cut(s) 65, 221, 272
RsaNI GTAC 3 cut(s) 64, 220, 271
Rsr2I CGGWCCG 1 cut(s) 352
RsrII CGGWCCG 1 cut(s) 352
SatI GCNGC 8 cut(s) 190, 266, 316, 522, 541, 579, 709, 742
Sau3AI GATC 7 cut(s) 274, 279, 406, 411, 507, 532, 735
Sau96I GGNCC 2 cut(s) 352, 478
SchI GAGTC 2 cut(s) 75, 448
ScrFI CCNGG 3 cut(s) 140, 473, 518
SfaNI GCATC 1 cut(s) 327
SinI GGWCC 2 cut(s) 352, 478
Sse9I AATT 1 cut(s) 346
StyD4I CCNGG 3 cut(s) 138, 471, 516
StyI CCWWGG 2 cut(s) 76, 501
TaaI ACNGT 2 cut(s) 607, 625
TaiI ACGT 5 cut(s) 252, 385, 567, 576, 651
TaqI TCGA 9 cut(s) 45, 108, 228, 405, 423, 452, 531, 555, 759
TasI AATT 1 cut(s) 346
TatI WGTACW 1 cut(s) 63
TauI GCSGC 3 cut(s) 543, 711, 744
TfiI GAWTC 4 cut(s) 5, 32, 135, 767
TseI GCWGC 5 cut(s) 189, 265, 315, 521, 578
TspDTI ATGAA 2 cut(s) 383, 446
TspGWI ACGGA 1 cut(s) 760
VpaK11BI GGWCC 2 cut(s) 352, 478
XapI RAATTY 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 598
ZraI GACGTC 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.