AT2G32410

E1 enzyme. E1 activates RUB1 NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a RUB1-ECR1 thioester and free AMP. E1 finally transfers RUB1 to the catalytic cysteine of RCE1

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
Physical Location & Seq
Forward (+)
13757593 .. 13761152
3560 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32410.3

Sequence Viewer

Length: 1572 bp
ATGGCGGAACCCAAAACCAAGTATGATCGTCAGCTAAGGATTTGGGGAGAATTAGGTCAATCAGCTCTTGAAACCGCGAGCATATGTTTGCTCAATTGTGGTCCAACTGGCTCTGAGGCTCTAAAGAATCTTGTTATTGGTGGAATCGGAAGTATCACCATTGTCGACGGATCTAAAGTAGAAATTGGCGACCTTGGAAACAATTTCATGGTGGATGCGAAGAGCGTTGGTCAGTCAAGAGCCAAAACGGTCTGTGGCTTTCTTCAGGAGCTTAACGATTCTGTTAAAGCAAATTTTGTTGAGGAGAATCCAGACACTTTGATATCGACAGACCCATCTTTCTTCTCTCAGTTCACTCTAGTTATCGCCACTCAGCTAGTAGAAGATTCAATGGTGAAATTAGATAGAATCTGCCGGGAAGCAAACGTCATGTTGGTTTTGGCTCGCTCCTATGGACTTACTGGGTTTGTTCGTATCAGTGTAAAGGAACACACCGCTATTGAGACAAAGCCTGATCATTCTCTTGATGATCTCCGCTTGAATAGTCCATGGCCTGAGCTGAAGAGCTATGTGGAGTCCATTGATCTAAATGTAGAAGAACCCGCTGCCCATAAGCACATTCCTTACGTCGTCATTCTAGTCAAGGTGGCTGAAGAATGGGCTCAACACCATAGCGGCAACCTTCCATCAACCAGGGAAGAGAAAAACGAGTTTAAGGACTTAGTTAAGTCCAAGATGGTATCAGCGGATGAAGAAAATTACAAAGAAGCCCTTCTTGCAGCTTTCAAAGTCTTTGCTCCTACAGGAATCAGCCAAGAGATTCAAGACATTAATCATGATAGTTGTGCTGAAGTTGGCTCAAATTCCTCAGATTTTTGGGTAATGGTAGCAGCACTCAAGGAGTTTATTTCAAATGAAGGTGGTGGGGAGGTACCACTTGAAGGTTCCATGCCTGATATGATATCTTCCACAGAGCACTACATCAATTTGCAGAAAATCTACCATTCCAAAGCGGAAGCTGATTTTCTTTCCATGGAACAGAGAGTAAAAAGTATTCTAGTTAAAGTTGGTCAGGATCCAAGTAGCATCTCAAAGCCAACTATCAAGAGCTTCTGCAAGAATGCACGAAAACTTAAAGTCTGCAGATATAGAACGATAGAAGACGAGTTCAAAAGTCCTTCTACAACAGAACTACATAAGTACTTGGCTGATGAGAATTACAGTGGCGCAATTGGATTTTATATTCTTCTTAGAGCTGTTGATAGATTTGCTGGTACCTATAAGAAGTTTCCTGGACAGTTTGATGGATCAACTGATGAGGACGCATCCCAGTTAAAGACTATTGCCCTGAGTCTTCTTAGTGAAATGGGTTGTGACGGCTATGAACTTCAGGAAGAACTGTACAATGAGATGTGTAGATTTGGTGCTGCAGAGATTCACGTGGTTGCTGCTTTAATAGGAGGAATCACATCTCAAGAAGTGATCAAGCTCATCACAAAGCAGTTTGTTCCCAAGCGAGGAACTTTCATCTTCAACGGCATTGATCACAAGTCTCAGTCTTTGACATTATAG

Protein Analysis

523

Amino Acids

57.94

Weight (kDa)

5.2

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ThiF PF00899 8 - 166 1.2e-20 ThiF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 931, 1274
AccB1I GGYRCC 2 cut(s) 931, 1274
AccI GTMKAC 1 cut(s) 165
AccII CGCG 1 cut(s) 77
AciI CCGC 8 cut(s) 5, 75, 495, 535, 603, 675, 746, 1013
AclWI GGATC 4 cut(s) 178, 1070, 1083, 1315
AcsI RAATTY 2 cut(s) 292, 862
AcuI CTGAAG 5 cut(s) 248, 581, 672, 870, 1373
AcvI CACGTG 1 cut(s) 1441
AfaI GTAC 4 cut(s) 933, 1202, 1276, 1403
AfiI CCNNNNNNNGG 2 cut(s) 941, 1517
AgsI TTSAA 9 cut(s) 71, 390, 541, 787, 824, 912, 941, 1171, 1534
AjnI CCWGG 2 cut(s) 692, 1291
Alw21I GWGCWC 1 cut(s) 978
Alw26I GTCTC 2 cut(s) 497, 1557
AlwI GGATC 4 cut(s) 178, 1070, 1083, 1315
AoxI GGCC 1 cut(s) 551
ApeKI GCWGC 5 cut(s) 605, 779, 890, 1427, 1448
ApoI RAATTY 2 cut(s) 292, 862
AseI ATTAAT 1 cut(s) 831
Asp700I GAANNNNTTC 1 cut(s) 771
Asp718I GGTACC 2 cut(s) 931, 1274
AspLEI GCGC 1 cut(s) 1229
AspS9I GGNCC 1 cut(s) 101
AsuC2I CCSGG 1 cut(s) 416
AsuHPI GGTGA 2 cut(s) 148, 406
AvaII GGWCC 1 cut(s) 101
BamHI GGATCC 1 cut(s) 1075
BanI GGYRCC 2 cut(s) 931, 1274
BanII GRGCYC 1 cut(s) 664
BbrPI CACGTG 1 cut(s) 1441
BbsI GAAGAC 2 cut(s) 1167, 1346
Bbv12I GWGCWC 1 cut(s) 978
BbvI GCAGC 5 cut(s) 592, 791, 902, 1414, 1435
BccI CCATC 4 cut(s) 343, 694, 730, 1298
BceAI ACGGC 2 cut(s) 1393, 1552
BciT130I CCWGG 2 cut(s) 694, 1293
BclI TGATCA 3 cut(s) 514, 1482, 1543
BcnI CCSGG 1 cut(s) 416
BcoDI GTCTC 2 cut(s) 497, 1557
BfaI CTAG 4 cut(s) 359, 377, 638, 1058
BfmI CTRYAG 3 cut(s) 801, 1141, 1428
BisI GCNGC 6 cut(s) 606, 676, 780, 891, 1428, 1449
BlsI GCNGC 6 cut(s) 607, 677, 781, 892, 1429, 1450
BmcAI AGTACT 1 cut(s) 1202
Bme1390I CCNGG 3 cut(s) 416, 694, 1293
Bme18I GGWCC 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 5 cut(s) 9, 933, 946, 1077, 1276
BmrFI CCNGG 3 cut(s) 416, 694, 1293
BmrI ACTGGG 2 cut(s) 471, 1324
BmsI GCATC 3 cut(s) 205, 1095, 1334
BmuI ACTGGG 2 cut(s) 471, 1324
BpiI GAAGAC 2 cut(s) 1167, 1346
Bpu10I CCTNAGC 2 cut(s) 35, 555
BpuEI CTTGAG 2 cut(s) 881, 1458
BpuMI CCSGG 1 cut(s) 416
BsaAI YACGTR 1 cut(s) 1441
BsaJI CCNNGG 4 cut(s) 193, 548, 693, 1032
Bsc4I CCNNNNNNNGG 2 cut(s) 941, 1517
Bse1I ACTGG 3 cut(s) 112, 466, 1330
BseBI CCWGG 2 cut(s) 694, 1293
BseDI CCNNGG 4 cut(s) 193, 548, 693, 1032
BseGI GGATG 3 cut(s) 220, 754, 1325
BseLI CCNNNNNNNGG 2 cut(s) 941, 1517
BseMII CTCAG 7 cut(s) 105, 362, 386, 546, 882, 1340, 1568
BseNI ACTGG 3 cut(s) 112, 466, 1330
BseRI GAGGAG 1 cut(s) 317
BseXI GCAGC 5 cut(s) 592, 791, 902, 1414, 1435
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 1 cut(s) 553
BshNI GGYRCC 2 cut(s) 931, 1274
BsiHKAI GWGCWC 1 cut(s) 978
BsiSI CCGG 1 cut(s) 415
BslI CCNNNNNNNGG 2 cut(s) 941, 1517
BsmAI GTCTC 2 cut(s) 497, 1557
BsmI GAATGC 1 cut(s) 1126
BsnI GGCC 1 cut(s) 553
Bsp1286I GDGCHC 2 cut(s) 664, 978
Bsp1407I TGTACA 1 cut(s) 1401
Bsp143I GATC 9 cut(s) 25, 170, 514, 529, 583, 1075, 1307, 1482, 1543
Bsp19I CCATGG 2 cut(s) 548, 1032
BspACI CCGC 8 cut(s) 5, 75, 495, 535, 603, 675, 746, 1013
BspANI GGCC 1 cut(s) 553
BspCNI CTCAG 7 cut(s) 106, 361, 385, 547, 881, 1341, 1567
BspFNI CGCG 1 cut(s) 77
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 5 cut(s) 9, 933, 946, 1077, 1276
BspMAI CTGCAG 2 cut(s) 1145, 1432
BspPI GGATC 4 cut(s) 178, 1070, 1083, 1315
BspQI GCTCTTC 2 cut(s) 215, 557
BspT107I GGYRCC 2 cut(s) 931, 1274
BsrGI TGTACA 1 cut(s) 1401
BsrI ACTGG 3 cut(s) 112, 466, 1330
BssECI CCNNGG 4 cut(s) 193, 548, 693, 1032
BssMI GATC 9 cut(s) 25, 170, 514, 529, 583, 1075, 1307, 1482, 1543
BssT1I CCWWGG 3 cut(s) 193, 548, 1032
Bst2UI CCWGG 2 cut(s) 694, 1293
Bst4CI ACNGT 4 cut(s) 250, 1223, 1299, 1401
Bst6I CTCTTC 3 cut(s) 215, 557, 693
BstAUI TGTACA 1 cut(s) 1401
BstBAI YACGTR 1 cut(s) 1441
BstC8I GCNNGC 2 cut(s) 79, 445
BstDSI CCRYGG 2 cut(s) 548, 1032
BstF5I GGATG 3 cut(s) 220, 754, 1325
BstFNI CGCG 1 cut(s) 77
BstHHI GCGC 1 cut(s) 1229
BstKTI GATC 9 cut(s) 28, 173, 517, 532, 586, 1078, 1310, 1485, 1546
BstMAI GTCTC 2 cut(s) 497, 1557
BstMBI GATC 9 cut(s) 25, 170, 514, 529, 583, 1075, 1307, 1482, 1543
BstMWI GCNNNNNNNGC 1 cut(s) 776
BstNI CCWGG 2 cut(s) 694, 1293
BstSCI CCNGG 3 cut(s) 414, 692, 1291
BstSFI CTRYAG 3 cut(s) 801, 1141, 1428
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 5 cut(s) 592, 791, 902, 1414, 1435
BstV2I GAAGAC 2 cut(s) 1167, 1346
BstX2I RGATCY 2 cut(s) 170, 1075
BstYI RGATCY 2 cut(s) 170, 1075
BsuRI GGCC 1 cut(s) 553
BtgI CCRYGG 2 cut(s) 548, 1032
BtsCI GGATG 3 cut(s) 220, 754, 1325
BtsIMutI CAGTG 2 cut(s) 484, 1228
Cac8I GCNNGC 2 cut(s) 79, 445
CciI TCATGA 1 cut(s) 835
CfoI GCGC 1 cut(s) 1229
Cfr13I GGNCC 1 cut(s) 101
CseI GACGC 1 cut(s) 1331
Csp6I GTAC 4 cut(s) 932, 1201, 1275, 1402
CviAII CATG 6 cut(s) 208, 430, 549, 836, 949, 1033
CviQI GTAC 4 cut(s) 932, 1201, 1275, 1402
DpnI GATC 9 cut(s) 27, 172, 516, 531, 585, 1077, 1309, 1484, 1545
DpnII GATC 9 cut(s) 25, 170, 514, 529, 583, 1075, 1307, 1482, 1543
Eam1104I CTCTTC 3 cut(s) 215, 557, 693
EarI CTCTTC 3 cut(s) 215, 557, 693
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 193, 548, 1032
Eco24I GRGCYC 1 cut(s) 664
Eco32I GATATC 2 cut(s) 324, 963
Eco47I GGWCC 1 cut(s) 101
Eco57I CTGAAG 5 cut(s) 248, 581, 672, 870, 1373
Eco72I CACGTG 1 cut(s) 1441
EcoRII CCWGG 2 cut(s) 692, 1291
EcoRV GATATC 2 cut(s) 324, 963
EcoT14I CCWWGG 3 cut(s) 193, 548, 1032
EcoT38I GRGCYC 1 cut(s) 664
ErhI CCWWGG 3 cut(s) 193, 548, 1032
FaeI CATG 6 cut(s) 211, 433, 552, 839, 952, 1036
FalI AAGNNNNNCTT 4 cut(s) 756, 788, 759, 791
FatI CATG 6 cut(s) 207, 429, 548, 835, 948, 1032
FauI CCCGC 1 cut(s) 610
FauNDI CATATG 1 cut(s) 83
FbaI TGATCA 3 cut(s) 514, 1482, 1543
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 6 cut(s) 606, 676, 780, 891, 1428, 1449
FokI GGATG 3 cut(s) 227, 761, 1312
FriOI GRGCYC 1 cut(s) 664
Fsp4HI GCNGC 6 cut(s) 606, 676, 780, 891, 1428, 1449
FspBI CTAG 4 cut(s) 359, 377, 638, 1058
GlaI GCGC 1 cut(s) 1228
GluI GCNGC 6 cut(s) 606, 676, 780, 891, 1428, 1449
HaeIII GGCC 1 cut(s) 553
HapII CCGG 1 cut(s) 415
HgaI GACGC 1 cut(s) 1331
HhaI GCGC 1 cut(s) 1229
Hin1II CATG 6 cut(s) 211, 433, 552, 839, 952, 1036
Hin6I GCGC 1 cut(s) 1227
HinP1I GCGC 1 cut(s) 1227
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HpaII CCGG 1 cut(s) 415
HphI GGTGA 2 cut(s) 148, 406
Hpy166II GTNNAC 2 cut(s) 166, 354
Hpy188I TCNGA 3 cut(s) 115, 149, 871
Hpy8I GTNNAC 2 cut(s) 166, 354
Hpy99I CGWCG 2 cut(s) 170, 632
HpyAV CCTTC 5 cut(s) 692, 782, 911, 935, 1188
HpyCH4III ACNGT 4 cut(s) 250, 1223, 1299, 1401
HpyCH4IV ACGT 3 cut(s) 426, 627, 1440
HpyCH4V TGCA 6 cut(s) 779, 991, 1116, 1124, 1143, 1430
HpyF10VI GCNNNNNNNGC 1 cut(s) 776
HpySE526I ACGT 3 cut(s) 426, 627, 1440
Hsp92II CATG 6 cut(s) 211, 433, 552, 839, 952, 1036
HspAI GCGC 1 cut(s) 1227
KpnI GGTACC 2 cut(s) 935, 1278
Ksp22I TGATCA 3 cut(s) 514, 1482, 1543
Kzo9I GATC 9 cut(s) 25, 170, 514, 529, 583, 1075, 1307, 1482, 1543
LguI GCTCTTC 2 cut(s) 215, 557
LmnI GCTCC 3 cut(s) 268, 452, 802
Lsp1109I GCAGC 5 cut(s) 592, 791, 902, 1414, 1435
LweI GCATC 3 cut(s) 205, 1095, 1334
MaeI CTAG 4 cut(s) 359, 377, 638, 1058
MaeII ACGT 3 cut(s) 426, 627, 1440
MaeIII GTNAC 1 cut(s) 1373
MalI GATC 9 cut(s) 27, 172, 516, 531, 585, 1077, 1309, 1484, 1545
MboI GATC 9 cut(s) 25, 170, 514, 529, 583, 1075, 1307, 1482, 1543
MfeI CAATTG 2 cut(s) 94, 1230
MflI RGATCY 2 cut(s) 170, 1075
MhlI GDGCHC 2 cut(s) 664, 978
MlyI GAGTC 2 cut(s) 584, 1360
MmeI TCCRAC 1 cut(s) 128
MnlI CCTC 7 cut(s) 109, 295, 877, 922, 1312, 1454, 1511
MroXI GAANNNNTTC 1 cut(s) 771
MseI TTAA 9 cut(s) 273, 285, 714, 726, 831, 1062, 1134, 1334, 1454
MspA1I CMGCKG 2 cut(s) 605, 746
MspI CCGG 1 cut(s) 415
MspR9I CCNGG 3 cut(s) 416, 694, 1293
MunI CAATTG 2 cut(s) 94, 1230
Mva1269I GAATGC 1 cut(s) 1126
MvaI CCWGG 2 cut(s) 694, 1293
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 1 cut(s) 776
NciI CCSGG 1 cut(s) 416
NcoI CCATGG 2 cut(s) 548, 1032
NdeI CATATG 1 cut(s) 83
NdeII GATC 9 cut(s) 25, 170, 514, 529, 583, 1075, 1307, 1482, 1543
NlaIII CATG 6 cut(s) 211, 433, 552, 839, 952, 1036
NlaIV GGNNCC 5 cut(s) 9, 933, 946, 1077, 1276
NmuCI GTSAC 1 cut(s) 1373
PagI TCATGA 1 cut(s) 835
PciSI GCTCTTC 2 cut(s) 215, 557
PctI GAATGC 1 cut(s) 1126
PdmI GAANNNNTTC 1 cut(s) 771
PfoI TCCNGGA 1 cut(s) 1291
PkrI GCNGC 6 cut(s) 607, 677, 781, 892, 1429, 1450
PleI GAGTC 2 cut(s) 583, 1359
PmaCI CACGTG 1 cut(s) 1441
PmlI CACGTG 1 cut(s) 1441
PpsI GAGTC 2 cut(s) 583, 1359
Ppu21I YACGTR 1 cut(s) 1441
PshBI ATTAAT 1 cut(s) 831
Psp6I CCWGG 2 cut(s) 692, 1291
PspCI CACGTG 1 cut(s) 1441
PspGI CCWGG 2 cut(s) 692, 1291
PspN4I GGNNCC 5 cut(s) 9, 933, 946, 1077, 1276
PspPI GGNCC 1 cut(s) 101
PstI CTGCAG 2 cut(s) 1145, 1432
PsuI RGATCY 2 cut(s) 170, 1075
RsaI GTAC 4 cut(s) 933, 1202, 1276, 1403
RsaNI GTAC 4 cut(s) 932, 1201, 1275, 1402
SalI GTCGAC 1 cut(s) 164
SapI GCTCTTC 2 cut(s) 215, 557
SaqAI TTAA 9 cut(s) 273, 285, 714, 726, 831, 1062, 1134, 1334, 1454
SatI GCNGC 6 cut(s) 606, 676, 780, 891, 1428, 1449
Sau3AI GATC 9 cut(s) 25, 170, 514, 529, 583, 1075, 1307, 1482, 1543
Sau96I GGNCC 1 cut(s) 101
ScaI AGTACT 1 cut(s) 1202
SchI GAGTC 2 cut(s) 584, 1360
ScrFI CCNGG 3 cut(s) 416, 694, 1293
SduI GDGCHC 2 cut(s) 664, 978
SfaNI GCATC 3 cut(s) 205, 1095, 1334
SfcI CTRYAG 3 cut(s) 801, 1141, 1428
SinI GGWCC 1 cut(s) 101
SmlI CTYRAG 2 cut(s) 896, 1473
SmoI CTYRAG 2 cut(s) 896, 1473
SsiI CCGC 8 cut(s) 5, 75, 495, 535, 603, 675, 746, 1013
SspMI CTAG 4 cut(s) 359, 377, 638, 1058
StyD4I CCNGG 3 cut(s) 414, 692, 1291
StyI CCWWGG 3 cut(s) 193, 548, 1032
TaaI ACNGT 4 cut(s) 250, 1223, 1299, 1401
TaiI ACGT 3 cut(s) 429, 630, 1443
TaqI TCGA 2 cut(s) 165, 326
TatI WGTACW 2 cut(s) 1200, 1401
TauI GCSGC 1 cut(s) 678
Tru1I TTAA 9 cut(s) 273, 285, 714, 726, 831, 1062, 1134, 1334, 1454
Tru9I TTAA 9 cut(s) 273, 285, 714, 726, 831, 1062, 1134, 1334, 1454
TscAI CASTG 2 cut(s) 484, 1228
TseFI GTSAC 1 cut(s) 1373
TseI GCWGC 5 cut(s) 605, 779, 890, 1427, 1448
Tsp45I GTSAC 1 cut(s) 1373
TspDTI ATGAA 5 cut(s) 196, 765, 930, 1398, 1516
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 2 cut(s) 484, 1228
VpaK11BI GGWCC 1 cut(s) 101
VspI ATTAAT 1 cut(s) 831
XapI RAATTY 2 cut(s) 292, 862
XmiI GTMKAC 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 4 cut(s) 359, 377, 638, 1058
ZrmI AGTACT 1 cut(s) 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.