Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0008502

Physical Location & Seq

Genomic Coordinates Forward (+)

12349000 .. 12354968

Gene Structure 5969 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00090640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

ATGGCTGAACCCAAAACCAAATACGATCGTCAGCTCAGAATCTGGGGCGAGCAGGGCCAAACAGCGCTCGAGAAAGCCAGCGTTTGCTTACTCAATTGCGGCCCTACTGGCTCCGAAACCCTCAAGAACCTCGTTCTCGGCGGCGTCGGAAGCATCACCGTCATTGATGGATCTAAAGTCGAATACGGCGACCTTGGAAACAACTTTATGGTTGATGAATCGAGCGTCGGGCAATCCAAGGCGAAATGTGTGTGTCAATTTCTTCAGGAGCTCAATGATGCTGTTAAGGCTAAGTTTATAGAGGAGTATCCAGAGGCTTTGATTCAGACCAACCCCTCCTTCTTTTCTCAGTTTACCTTGGTTGTGGCCACTCAGCTGGTGGAAGATTCAATGGTGAAGCTCGACAGAATATGTAGGGATGCAAATGTGCTATTGATCTTTGCTCGCTCTTATGGCCTCACTGGGCTTGTCCGTATCAGTTTGAAGCAGGAACATACTGTGATTGAGTCCAAGCCTGAACATTTCCTGGATGACCTTCGTTTGAATAATCCATGGCCTGAGCTTAGAGGGTTTGCGGAGACCATTGATCTAGATGTGTCAGATCCTGTTGCCCACAAACACATGCCATATGTTGTCATTCTTGTCAAGATGGCACAGGAGTGGGCTAAAAGTCACGGTGGTAAACTTCCAACAACTAGGGAAGAGAAAAAAGAGTTCAAGGAGCTTATCAAAGCTAGGATGACTGCAATTGATGAAGACAACTACAGAGAAGCTATTGAGAACTCCTTTAAAGTCTTCACTCCTCGAGGAATCAGTCCAGAGTTGCAACAGATAATTAGTGACAGCCGTTCTGAAATTGATTCCAGTTCATCAGACTTTTGGGTGATGGTGGCGGCATTGAAGGTTTCTTTAGAGACAAGTGTCAGGCATGTCATTTTGACTGTGAAACTGCAATATGTTGCCTTACAGAAAATCTACCAAGCCAAGGCTGAGGCTGATTTTCTAGTTATTGACCAAAGGGTTAGGAGTCTTCTAAAGAAAATTGGTAGAGATCCTAGTAGCATCTCAAAGGCAACTGTTAAAAGTTTCTGCAAAAATGCAAGAAAACTCAAAGTATCTCTCTCCAAGAAGTATCTTTTAGCTAAGAATGCTGTTTTAGTGTATAGCTACAAATTGAAATTAGGGCTCAATATTGTGGTTTGCCGGTACCGCCCCATTGAAGATGAGTTCAGTTCTCCAATCCTACCAGACTTACAGAAGTATCTAACAGATGAGGATTACAGTGTAGCTGGTGGGTTTTACATTTTGCTTAGAGCTGTTGATCGGTTTGCTGCTAATTATAACAGTTTCCCCGGAAAATTTGAAGGTGTGATGGATGAGGACATATCTAGGTTGAAGACAACAGCTGTCAGCCTTCTTAGTGATCTGGGTTGCAATGGCGTAACATTGACAGAAGACCTTATCAATGAGATGTGCCGATTTGGTGCTGCAGAGCTCCATGCAGTTGCTGCTTTCATTGGAGGAATTGCATCTCAGGAAGTGATTAAGCTTATAACGAGACAATTTGTACCCTTGTCTGGGACTTTCATCTTCAATGGCATCGACCACAAGTCTCAATTGTTGGCACTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006310 GO:0006464 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007127 GO:0007131 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008641 GO:0009414 GO:0009415 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009735 GO:0009755 GO:0009791 GO:0009965 GO:0009987 GO:0010016 GO:0010033 GO:0010035 GO:0010252 GO:0016043 GO:0016874 GO:0016877 GO:0019538 GO:0019781 GO:0022402 GO:0022414 GO:0023052 GO:0032446 GO:0032501 GO:0032502 GO:0032870 GO:0033554 GO:0034641 GO:0035825 GO:0036211 GO:0042221 GO:0042579 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045116 GO:0046483 GO:0048285 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0048878 GO:0050789 GO:0050794 GO:0050896 GO:0051321 GO:0051716 GO:0061982 GO:0065007 GO:0065008 GO:0070647 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0071840 GO:0090304 GO:0099402 GO:0140013 GO:1901360 GO:1901564 GO:1901700 GO:1903046 GO:1905392

KEGG Pathways

Metabolic & Signaling

ko05010 map05010

Pfam Domains

Protein Families

ThiF

Protein Analysis

543

Amino Acids

60.67

Weight (kDa)

6.74

Isoelectric Point (pI)

34.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ThiF	PF00899	8 - 522	9.2e-26	ThiF family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008502)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G05180 AT1G05180 AT1G05180 AT2G32410
fragaria_vesca	FvH4_6g43170 FvH4_6g43170 FvH4_6g43170
malus_domestica	MD09G1109000.v1.1 MD17G1097000.v1.1
prunus_persica	Prupe.3G217100_v2.0.a1
pyrus_communis	pycom09g03150 pycom17g09280
rosa_chinensis	RchiOBHm_Chr2g0159271
rosa_laevigata	RLG00000021149
rosa_multiflora	Rmu_sc0016200.1_g000007
rosa_roxburghii	Rroxscaffold_2G00090640
rosa_rugosa	Rorug02G0475500 Rorug02G0475500 Rorug02G0475600
rosa_samantha	Rh2AG541900 Rh2CG524600 Rh2DG564300
rosa_wichuraiana	Rw2G044820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1341, 1553
AbsI	CCTCGAGG	1 cut(s)	804
Acc65I	GGTACC	1 cut(s)	1206
AccB1I	GGYRCC	1 cut(s)	1206
AciI	CCGC	5 cut(s)	99, 141, 575, 893, 1210
AclWI	GGATC	3 cut(s)	178, 596, 1046
AcoI	YGGCCR	1 cut(s)	366
AcsI	RAATTY	1 cut(s)	1358
AcuI	CTGAAG	1 cut(s)	248
AcyI	GRCGYC	1 cut(s)	144
AfaI	GTAC	2 cut(s)	1208, 1569
AfeI	AGCGCT	1 cut(s)	66
AfiI	CCNNNNNNNGG	3 cut(s)	985, 1577, 1578
AhdI	GACNNNNNGTC	1 cut(s)	1609
AjnI	CCWGG	1 cut(s)	525
AjuI	GAANNNNNNNTTGG	2 cut(s)	323, 355
AleI	CACNNNNGTG	1 cut(s)	658
Alw21I	GWGCWC	2 cut(s)	273, 1497
Alw26I	GTCTC	4 cut(s)	572, 908, 1552, 1617
AlwI	GGATC	3 cut(s)	178, 596, 1046
AlwNI	CAGNNNCTG	3 cut(s)	42, 605, 1508
Ama87I	CYCGRG	2 cut(s)	68, 804
Aor51HI	AGCGCT	1 cut(s)	66
AoxI	GGCC	5 cut(s)	55, 100, 366, 454, 554
ApeKI	GCWGC	3 cut(s)	1331, 1487, 1508
ApoI	RAATTY	1 cut(s)	1358
Asp718I	GGTACC	1 cut(s)	1206
AspLEI	GCGC	1 cut(s)	67
AspS9I	GGNCC	2 cut(s)	55, 101
AsuC2I	CCSGG	1 cut(s)	1353
AsuHPI	GGTGA	3 cut(s)	148, 406, 895
AvaI	CYCGRG	2 cut(s)	68, 804
BaeI	ACNNNNGTAYC	2 cut(s)	1244, 1277
BalI	TGGCCA	1 cut(s)	368
BanI	GGYRCC	1 cut(s)	1206
BanII	GRGCYC	3 cut(s)	273, 1188, 1497
BbsI	GAAGAC	5 cut(s)	762, 787, 1022, 1403, 1461
Bbv12I	GWGCWC	2 cut(s)	273, 1497
BbvCI	CCTCAGC	1 cut(s)	990
BbvI	GCAGC	3 cut(s)	1318, 1474, 1495
BccI	CCATC	4 cut(s)	161, 643, 880, 1366
BceAI	ACGGC	2 cut(s)	202, 831
BciT130I	CCWGG	1 cut(s)	527
BciVI	GTATCC	1 cut(s)	318
BcnI	CCSGG	1 cut(s)	1353
BcoDI	GTCTC	4 cut(s)	572, 908, 1552, 1617
BfaI	CTAG	6 cut(s)	590, 696, 735, 1004, 1056, 1389
BfmI	CTRYAG	3 cut(s)	763, 1488, 1628
BfoI	RGCGCY	1 cut(s)	68
BfuI	GTATCC	1 cut(s)	318
BglI	GCCNNNNNGGC	1 cut(s)	108
BisI	GCNGC	6 cut(s)	100, 142, 894, 1332, 1488, 1509
BlsI	GCNGC	6 cut(s)	101, 143, 895, 1333, 1489, 1510
Bme1390I	CCNGG	2 cut(s)	527, 1353
BmeRI	GACNNNNNGTC	1 cut(s)	1609
BmeT110I	CYCGRG	2 cut(s)	68, 804
BmgT120I	GGNCC	2 cut(s)	55, 101
BmiI	GGNNCC	2 cut(s)	112, 1208
BmrFI	CCNGG	2 cut(s)	527, 1353
BmrI	ACTGGG	1 cut(s)	471
BmsI	GCATC	6 cut(s)	162, 268, 409, 1071, 1538, 1608
BmuI	ACTGGG	1 cut(s)	471
BoxI	GACNNNNGTC	1 cut(s)	920
BpiI	GAAGAC	5 cut(s)	762, 787, 1022, 1403, 1461
Bpu10I	CCTNAGC	2 cut(s)	558, 990
BpuEI	CTTGAG	1 cut(s)	107
BpuMI	CCSGG	1 cut(s)	1353
BsaHI	GRCGYC	1 cut(s)	144
BsaI	GGTCTC	1 cut(s)	572
BsaJI	CCNNGG	6 cut(s)	193, 237, 357, 551, 984, 1351
Bsc4I	CCNNNNNNNGG	3 cut(s)	985, 1577, 1578
Bse118I	RCCGGY	1 cut(s)	1203
Bse1I	ACTGG	3 cut(s)	112, 466, 864
Bse3DI	GCAATG	1 cut(s)	1441
BseBI	CCWGG	1 cut(s)	527
BseDI	CCNNGG	6 cut(s)	193, 237, 357, 551, 984, 1351
BseGI	GGATG	4 cut(s)	424, 535, 744, 1381
BseLI	CCNNNNNNNGG	3 cut(s)	985, 1577, 1578
BseMI	GCAATG	1 cut(s)	1441
BseMII	CTCAG	6 cut(s)	49, 362, 386, 549, 981, 1547
BseNI	ACTGG	3 cut(s)	112, 466, 864
BseRI	GAGGAG	2 cut(s)	317, 792
BseXI	GCAGC	3 cut(s)	1318, 1474, 1495
Bsh1285I	CGRYCG	1 cut(s)	28
BshFI	GGCC	5 cut(s)	57, 102, 368, 456, 556
BshNI	GGYRCC	1 cut(s)	1206
BsiEI	CGRYCG	1 cut(s)	28
BsiHKAI	GWGCWC	2 cut(s)	273, 1497
BsiHKCI	CYCGRG	2 cut(s)	68, 804
BsiSI	CCGG	2 cut(s)	1204, 1353
BslFI	GGGAC	1 cut(s)	1594
BslI	CCNNNNNNNGG	3 cut(s)	985, 1577, 1578
BsmAI	GTCTC	4 cut(s)	572, 908, 1552, 1617
BsmFI	GGGAC	1 cut(s)	1594
BsmI	GAATGC	1 cut(s)	1153
BsnI	GGCC	5 cut(s)	57, 102, 368, 456, 556
Bso31I	GGTCTC	1 cut(s)	572
BsoBI	CYCGRG	2 cut(s)	68, 804
Bsp1286I	GDGCHC	3 cut(s)	273, 1188, 1497
Bsp143I	GATC	8 cut(s)	25, 170, 435, 586, 601, 1051, 1321, 1423
Bsp19I	CCATGG	1 cut(s)	551
BspACI	CCGC	5 cut(s)	99, 141, 575, 893, 1210
BspANI	GGCC	5 cut(s)	57, 102, 368, 456, 556
BspCNI	CTCAG	6 cut(s)	48, 361, 385, 550, 982, 1546
BspLI	GGNNCC	2 cut(s)	112, 1208
BspMAI	CTGCAG	1 cut(s)	1492
BspPI	GGATC	3 cut(s)	178, 596, 1046
BspT107I	GGYRCC	1 cut(s)	1206
BspTNI	GGTCTC	1 cut(s)	572
BsrDI	GCAATG	1 cut(s)	1441
BsrFI	RCCGGY	1 cut(s)	1203
BsrI	ACTGG	3 cut(s)	112, 466, 864
BssAI	RCCGGY	1 cut(s)	1203
BssECI	CCNNGG	6 cut(s)	193, 237, 357, 551, 984, 1351
BssMI	GATC	8 cut(s)	25, 170, 435, 586, 601, 1051, 1321, 1423
BssNI	GRCGYC	1 cut(s)	144
BssT1I	CCWWGG	5 cut(s)	193, 237, 357, 551, 984
Bst2UI	CCWGG	1 cut(s)	527
Bst4CI	ACNGT	8 cut(s)	160, 499, 677, 943, 1078, 1283, 1346, 1629
Bst6I	CTCTTC	1 cut(s)	696
BstACI	GRCGYC	1 cut(s)	144
BstAPI	GCANNNNNTGC	1 cut(s)	1508
BstC8I	GCNNGC	3 cut(s)	50, 79, 445
BstDSI	CCRYGG	1 cut(s)	551
BstF5I	GGATG	4 cut(s)	424, 535, 744, 1381
BstH2I	RGCGCY	1 cut(s)	68
BstHHI	GCGC	1 cut(s)	67
BstKTI	GATC	8 cut(s)	28, 173, 438, 589, 604, 1054, 1324, 1426
BstMAI	GTCTC	4 cut(s)	572, 908, 1552, 1617
BstMBI	GATC	8 cut(s)	25, 170, 435, 586, 601, 1051, 1321, 1423
BstMCI	CGRYCG	1 cut(s)	28
BstMWI	GCNNNNNNNGC	8 cut(s)	54, 108, 150, 287, 453, 1148, 1209, 1508
BstNI	CCWGG	1 cut(s)	527
BstNSI	RCATGY	2 cut(s)	625, 932
BstPAI	GACNNNNGTC	1 cut(s)	920
BstSCI	CCNGG	2 cut(s)	525, 1351
BstSFI	CTRYAG	3 cut(s)	763, 1488, 1628
BstV1I	GCAGC	3 cut(s)	1318, 1474, 1495
BstV2I	GAAGAC	5 cut(s)	762, 787, 1022, 1403, 1461
BstX2I	RGATCY	3 cut(s)	170, 601, 1051
BstXI	CCANNNNNNTGG	1 cut(s)	376
BstYI	RGATCY	3 cut(s)	170, 601, 1051
BsuI	GTATCC	1 cut(s)	318
BsuRI	GGCC	5 cut(s)	57, 102, 368, 456, 556
BtgI	CCRYGG	1 cut(s)	551
BtsCI	GGATG	4 cut(s)	424, 535, 744, 1381
BtsIMutI	CAGTG	3 cut(s)	459, 1288, 1625
Cac8I	GCNNGC	3 cut(s)	50, 79, 445
CaiI	CAGNNNCTG	3 cut(s)	42, 605, 1508
CfoI	GCGC	1 cut(s)	67
Cfr10I	RCCGGY	1 cut(s)	1203
Cfr13I	GGNCC	2 cut(s)	55, 101
CseI	GACGC	2 cut(s)	133, 214
Csp6I	GTAC	2 cut(s)	1207, 1568
CviAII	CATG	4 cut(s)	552, 622, 929, 1499
CviQI	GTAC	2 cut(s)	1207, 1568
DpnI	GATC	8 cut(s)	27, 172, 437, 588, 603, 1053, 1323, 1425
DpnII	GATC	8 cut(s)	25, 170, 435, 586, 601, 1051, 1321, 1423
DraI	TTTAAA	1 cut(s)	790
DriI	GACNNNNNGTC	1 cut(s)	1609
EaeI	YGGCCR	1 cut(s)	366
Eam1104I	CTCTTC	1 cut(s)	696
Eam1105I	GACNNNNNGTC	1 cut(s)	1609
EarI	CTCTTC	1 cut(s)	696
Ecl136II	GAGCTC	2 cut(s)	271, 1495
Eco130I	CCWWGG	5 cut(s)	193, 237, 357, 551, 984
Eco24I	GRGCYC	3 cut(s)	273, 1188, 1497
Eco31I	GGTCTC	1 cut(s)	572
Eco47III	AGCGCT	1 cut(s)	66
Eco53kI	GAGCTC	2 cut(s)	271, 1495
Eco57I	CTGAAG	1 cut(s)	248
Eco88I	CYCGRG	2 cut(s)	68, 804
EcoICRI	GAGCTC	2 cut(s)	271, 1495
EcoRII	CCWGG	1 cut(s)	525
EcoT14I	CCWWGG	5 cut(s)	193, 237, 357, 551, 984
EcoT38I	GRGCYC	3 cut(s)	273, 1188, 1497
ErhI	CCWWGG	5 cut(s)	193, 237, 357, 551, 984
FaeI	CATG	4 cut(s)	555, 625, 932, 1502
FaqI	GGGAC	1 cut(s)	1594
FatI	CATG	4 cut(s)	551, 621, 928, 1498
FauNDI	CATATG	1 cut(s)	628
Fnu4HI	GCNGC	6 cut(s)	100, 142, 894, 1332, 1488, 1509
FokI	GGATG	4 cut(s)	431, 542, 751, 1388
FriOI	GRGCYC	3 cut(s)	273, 1188, 1497
Fsp4HI	GCNGC	6 cut(s)	100, 142, 894, 1332, 1488, 1509
FspBI	CTAG	6 cut(s)	590, 696, 735, 1004, 1056, 1389
GlaI	GCGC	1 cut(s)	66
GluI	GCNGC	6 cut(s)	100, 142, 894, 1332, 1488, 1509
HaeII	RGCGCY	1 cut(s)	68
HaeIII	GGCC	5 cut(s)	57, 102, 368, 456, 556
HapII	CCGG	2 cut(s)	1204, 1353
HgaI	GACGC	2 cut(s)	133, 214
HhaI	GCGC	1 cut(s)	67
Hin1I	GRCGYC	1 cut(s)	144
Hin1II	CATG	4 cut(s)	555, 625, 932, 1502
Hin6I	GCGC	1 cut(s)	65
HinP1I	GCGC	1 cut(s)	65
HindIII	AAGCTT	1 cut(s)	1547
HinfI	GANTC	8 cut(s)	39, 218, 322, 386, 506, 810, 860, 1027
HpaII	CCGG	2 cut(s)	1204, 1353
HphI	GGTGA	3 cut(s)	148, 406, 895
Hpy166II	GTNNAC	2 cut(s)	354, 683
Hpy188I	TCNGA	7 cut(s)	38, 115, 149, 327, 601, 853, 874
Hpy188III	TCNNGA	8 cut(s)	70, 124, 266, 311, 590, 646, 818, 1535
Hpy8I	GTNNAC	2 cut(s)	354, 683
Hpy99I	CGWCG	2 cut(s)	149, 230
HpyAV	CCTTC	5 cut(s)	349, 545, 895, 1358, 1424
HpyCH4III	ACNGT	8 cut(s)	160, 499, 677, 943, 1078, 1283, 1346, 1629
HpyF10VI	GCNNNNNNNGC	8 cut(s)	54, 108, 150, 287, 453, 1148, 1209, 1508
Hsp92I	GRCGYC	1 cut(s)	144
Hsp92II	CATG	4 cut(s)	555, 625, 932, 1502
HspAI	GCGC	1 cut(s)	65
KpnI	GGTACC	1 cut(s)	1210
Kzo9I	GATC	8 cut(s)	25, 170, 435, 586, 601, 1051, 1321, 1423
LmnI	GCTCC	4 cut(s)	116, 268, 721, 1500
Lsp1109I	GCAGC	3 cut(s)	1318, 1474, 1495
LweI	GCATC	6 cut(s)	162, 268, 409, 1071, 1538, 1608
MaeI	CTAG	6 cut(s)	590, 696, 735, 1004, 1056, 1389
MaeIII	GTNAC	3 cut(s)	671, 839, 1441
MalI	GATC	8 cut(s)	27, 172, 437, 588, 603, 1053, 1323, 1425
MboI	GATC	8 cut(s)	25, 170, 435, 586, 601, 1051, 1321, 1423
MfeI	CAATTG	3 cut(s)	94, 747, 1616
MflI	RGATCY	3 cut(s)	170, 601, 1051
MhlI	GDGCHC	3 cut(s)	273, 1188, 1497
MlsI	TGGCCA	1 cut(s)	368
MluNI	TGGCCA	1 cut(s)	368
MlyI	GAGTC	2 cut(s)	515, 1036
MmeI	TCCRAC	2 cut(s)	127, 713
Mox20I	TGGCCA	1 cut(s)	368
MscI	TGGCCA	1 cut(s)	368
MseI	TTAA	4 cut(s)	285, 789, 1080, 1545
MslI	CAYNNNNRTG	1 cut(s)	658
Msp20I	TGGCCA	1 cut(s)	368
MspA1I	CMGCKG	2 cut(s)	376, 1406
MspI	CCGG	2 cut(s)	1204, 1353
MspR9I	CCNGG	2 cut(s)	527, 1353
MunI	CAATTG	3 cut(s)	94, 747, 1616
Mva1269I	GAATGC	1 cut(s)	1153
MvaI	CCWGG	1 cut(s)	527
MwoI	GCNNNNNNNGC	8 cut(s)	54, 108, 150, 287, 453, 1148, 1209, 1508
NciI	CCSGG	1 cut(s)	1353
NcoI	CCATGG	1 cut(s)	551
NdeI	CATATG	1 cut(s)	628
NdeII	GATC	8 cut(s)	25, 170, 435, 586, 601, 1051, 1321, 1423
NlaIII	CATG	4 cut(s)	555, 625, 932, 1502
NlaIV	GGNNCC	2 cut(s)	112, 1208
NmeAIII	GCCGAG	1 cut(s)	117
NmuCI	GTSAC	2 cut(s)	671, 839
NspI	RCATGY	2 cut(s)	625, 932
OliI	CACNNNNGTG	1 cut(s)	658
PaeR7I	CTCGAG	2 cut(s)	68, 804
PcsI	WCGNNNNNNNCGW	1 cut(s)	186
PctI	GAATGC	1 cut(s)	1153
PfeI	GAWTC	6 cut(s)	39, 218, 322, 386, 810, 860
PfoI	TCCNGGA	1 cut(s)	525
PkrI	GCNGC	6 cut(s)	101, 143, 895, 1333, 1489, 1510
Ple19I	CGATCG	1 cut(s)	28
PleI	GAGTC	2 cut(s)	514, 1035
PpsI	GAGTC	2 cut(s)	514, 1035
PshAI	GACNNNNGTC	1 cut(s)	920
PsiI	TTATAA	2 cut(s)	1341, 1553
Psp124BI	GAGCTC	2 cut(s)	273, 1497
Psp6I	CCWGG	1 cut(s)	525
PspGI	CCWGG	1 cut(s)	525
PspN4I	GGNNCC	2 cut(s)	112, 1208
PspPI	GGNCC	2 cut(s)	55, 101
PspXI	VCTCGAGB	1 cut(s)	804
PstI	CTGCAG	1 cut(s)	1492
PstNI	CAGNNNCTG	3 cut(s)	42, 605, 1508
PsuI	RGATCY	3 cut(s)	170, 601, 1051
PvuI	CGATCG	1 cut(s)	28
PvuII	CAGCTG	2 cut(s)	376, 1406
RsaI	GTAC	2 cut(s)	1208, 1569
RsaNI	GTAC	2 cut(s)	1207, 1568
RseI	CAYNNNNRTG	1 cut(s)	658
SacI	GAGCTC	2 cut(s)	273, 1497
SaqAI	TTAA	4 cut(s)	285, 789, 1080, 1545
SatI	GCNGC	6 cut(s)	100, 142, 894, 1332, 1488, 1509
Sau3AI	GATC	8 cut(s)	25, 170, 435, 586, 601, 1051, 1321, 1423
Sau96I	GGNCC	2 cut(s)	55, 101
SchI	GAGTC	2 cut(s)	515, 1036
ScrFI	CCNGG	2 cut(s)	527, 1353
SduI	GDGCHC	3 cut(s)	273, 1188, 1497
SfaNI	GCATC	6 cut(s)	162, 268, 409, 1071, 1538, 1608
SfcI	CTRYAG	3 cut(s)	763, 1488, 1628
Sfr274I	CTCGAG	2 cut(s)	68, 804
SlaI	CTCGAG	2 cut(s)	68, 804
SmiMI	CAYNNNNRTG	1 cut(s)	658
SmlI	CTYRAG	3 cut(s)	68, 122, 804
SmoI	CTYRAG	3 cut(s)	68, 122, 804
SsiI	CCGC	5 cut(s)	99, 141, 575, 893, 1210
SspI	AATATT	1 cut(s)	1192
SspMI	CTAG	6 cut(s)	590, 696, 735, 1004, 1056, 1389
SstI	GAGCTC	2 cut(s)	273, 1497
StyD4I	CCNGG	2 cut(s)	525, 1351
StyI	CCWWGG	5 cut(s)	193, 237, 357, 551, 984
TaaI	ACNGT	8 cut(s)	160, 499, 677, 943, 1078, 1283, 1346, 1629
TaqI	TCGA	6 cut(s)	69, 180, 221, 402, 805, 1602
TauI	GCSGC	3 cut(s)	102, 144, 896
TfiI	GAWTC	6 cut(s)	39, 218, 322, 386, 810, 860
Tru1I	TTAA	4 cut(s)	285, 789, 1080, 1545
Tru9I	TTAA	4 cut(s)	285, 789, 1080, 1545
TscAI	CASTG	3 cut(s)	466, 1288, 1632
TseFI	GTSAC	2 cut(s)	671, 839
TseI	GCWGC	3 cut(s)	1331, 1487, 1508
Tsp45I	GTSAC	2 cut(s)	671, 839
TspDTI	ATGAA	5 cut(s)	231, 768, 858, 1504, 1576
TspGWI	ACGGA	1 cut(s)	461
TspRI	CASTG	3 cut(s)	466, 1288, 1632
XapI	RAATTY	1 cut(s)	1358
XbaI	TCTAGA	1 cut(s)	589
XceI	RCATGY	2 cut(s)	625, 932
XcmI	CCANNNNNNNNNTGG	1 cut(s)	376
XhoI	CTCGAG	2 cut(s)	68, 804
XspI	CTAG	6 cut(s)	590, 696, 735, 1004, 1056, 1389

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.