AT2G33630

3-beta hydroxysteroid dehydrogenase isomerase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012481
Physical Location & Seq
Reverse (-)
14236833 .. 14238877
2045 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33630.1

Sequence Viewer

Length: 1443 bp
ATGCATTTGAGTGAGAATGAAGGTGTCGAAGGTAACACCTTCGTGGTCACTGGAGGTCTTGGCTTCGTCGGTGCAGCTCTCTGCTTAGAGCTCGTTCGTCGTGGTGCTCGCCAAGTTCGCTCTTTTGACCTCCGTCACTCTTCTCCTTGGTCCGATGATCTCAAAAACAGTGGCGTTCGCTGCATTCAAGGTGATGTGACTAAGAAACAAGATGTGGATAACGCTTTAGATGGAGCAGACTGTGTTTTGCATCTTGCTTCTTATGGTATGTCTGGGAAGGAGATGCTTCGGTTTGGTCGTTGTGATGAGGTTAACATCAACGGGACTTGTAATGTCTTGGAAGCTGCGTTTAAACATGAGATTACAAGAATTGTGTATGTTAGCACTTACAATGTTGTGTTTGGTGGTAAAGAGATTCTCAATGGTAATGAAGGTTTGCCTTATTTCCCTCTTGATGATCATGTTGATGCATATAGTCGAACTAAATCGATTGCAGAACAGTTGGTTCTCAAGAGTAATGGTCGACCTTTTAAGAATGGAGGCAAACGGATGTATACATGTGCGATTCGTCCAGCGGCTATATATGGACCTGGCGAAGATAGGCATCTTCCTAGGATTGTTACTCTAACAAAGTTGGGTTTGGCTCTCTTCAAGATCGGTGAACCGAGTGTCAAATCAGACTGGATTTATGTCGAAAACCTTGTCCTAGCAATCATCTTGGCAAGTATGGGACTCTTGGATGACATTCCTGGCAGAGAAGGACAACCTGTTGCTGCTGGTCAACCATATTTTGTCTCTGACGGTTACCCGGTGAATACCTTTGAGTTTCTGCGTCCTTTACTAAAAAGTTTAGACTATGACCTGCCCAAGTGTACAATCTCTGTACCTTTTGCGCTGTCCTTGGGTAAGATCTTCCAAGGTTTCTACACTGTGCTATATCCATGGCTATCAAAGAGCTGGTTACCACAGCCCCTTGTTCTTCCTGCTGAAGTCTACAAGGTCGGTGTTACCCATTATTTCTCATATCTTAAAGCCAAGGAGGAGCTTGGATATGTTCCATTCAAGAGCTCCAAGGAAGGTATGGCTGCAACTATCTCATACTGGCAGGAAAGGAAGCGGAGATCTTTAGACGGTCCAACCATGTTCACTTGGATAGCTGTTACAATAGGAATGTCTGCTCTTTTTGCTGCCGGATGGTTACCCGACATAGGACCTGTGCCTTTCTTAAGAGCCATACACCTCTTCTTTTTCCGGACAATCACGATTGTAAAGGCTGTCTTCATTGTAGCAGTGGTACTACATGTTGCAGAAGGAATCTATGCGTGGTTTCTGGCTAAACGAGTCGACCCAGGAAATGCAATGGGGTGGTTCTTGCAAACCAGCGCTCTCGGGTTTTTCTCAATGCGGTTTTTATTGAAGAGAGCGAAAGAGCACCAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

480

Amino Acids

53.43

Weight (kDa)

8.86

Isoelectric Point (pI)

37.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 15 - 292 3.3e-98 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 15 - 241 6e-29 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 15 - 130 2.5e-08 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 16 - 240 1.8e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 16 - 175 5.3e-06 Male sterility protein
NAD_binding_10 PF13460 18 - 133 2e-12 NAD(P)H-binding
TMEM254 PF14934 387 - 472 2.8e-26 Transmembrane protein 254
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33630
fragaria_vesca FvH4_5g26130 FvH4_5g26130
malus_domestica MD06G1004300.v1.1
prunus_persica Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1
pyrus_communis pycom06g00410
rosa_chinensis RchiOBHm_Chr7g0216661
rosa_laevigata RLG00000002552
rosa_multiflora Rmu_sc0029435.1_g000001 Rmu_ssc0000117.1_g000009
rosa_roxburghii Rroxscaffold_3G00243030
rosa_rugosa Rorug07G0161500
rosa_samantha Rh7AG301300 Rh7BG292800 Rh7CG319900 Rh7DG301900
rosa_wichuraiana Rw7G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 870
AccI GTMKAC 4 cut(s) 523, 554, 993, 1344
AccIII TCCGGA 1 cut(s) 1251
AciI CCGC 3 cut(s) 575, 1117, 1405
AcuI CTGAAG 1 cut(s) 1008
AfaI GTAC 3 cut(s) 874, 885, 1296
AfeI AGCGCT 1 cut(s) 1384
AfiI CCNNNNNNNGG 1 cut(s) 1208
AflII CTTAAG 1 cut(s) 1225
AflIII ACRYGT 2 cut(s) 557, 1300
AgsI TTSAA 4 cut(s) 188, 652, 1063, 1417
AjnI CCWGG 3 cut(s) 589, 748, 1348
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 7 cut(s) 77, 91, 344, 957, 1045, 1068, 1157
AluI AGCT 7 cut(s) 77, 91, 344, 957, 1045, 1068, 1157
Alw21I GWGCWC 4 cut(s) 93, 109, 1070, 1434
Alw26I GTCTC 1 cut(s) 799
Ama87I CYCGRG 1 cut(s) 1388
Aor13HI TCCGGA 1 cut(s) 1251
Aor51HI AGCGCT 1 cut(s) 1384
ApeKI GCWGC 6 cut(s) 74, 180, 344, 773, 1085, 1187
ArsI GACNNNNNNTTYG 2 cut(s) 687, 719
AspA2I CCTAGG 1 cut(s) 611
AspLEI GCGC 2 cut(s) 895, 1385
AspS9I GGNCC 4 cut(s) 150, 587, 1133, 1211
AsuC2I CCSGG 1 cut(s) 809
AsuHPI GGTGA 3 cut(s) 203, 671, 823
AvaI CYCGRG 1 cut(s) 1388
AvaII GGWCC 4 cut(s) 150, 587, 1133, 1211
AvrII CCTAGG 1 cut(s) 611
BanII GRGCYC 2 cut(s) 93, 1070
BbsI GAAGAC 1 cut(s) 1270
Bbv12I GWGCWC 4 cut(s) 93, 109, 1070, 1434
BbvI GCAGC 6 cut(s) 86, 167, 331, 760, 1072, 1174
BccI CCATC 2 cut(s) 224, 1188
BciT130I CCWGG 3 cut(s) 591, 750, 1350
BclI TGATCA 1 cut(s) 457
BcnI CCSGG 1 cut(s) 809
BcoDI GTCTC 1 cut(s) 799
BfaI CTAG 2 cut(s) 612, 707
BfoI RGCGCY 1 cut(s) 1386
BfrI CTTAAG 1 cut(s) 1225
BfuAI ACCTGC 1 cut(s) 870
BglII AGATCT 2 cut(s) 909, 1121
BisI GCNGC 7 cut(s) 75, 181, 345, 576, 774, 1086, 1188
BlnI CCTAGG 1 cut(s) 611
BlsI GCNGC 7 cut(s) 76, 182, 346, 577, 775, 1087, 1189
Bme1390I CCNGG 4 cut(s) 591, 750, 809, 1350
Bme18I GGWCC 4 cut(s) 150, 587, 1133, 1211
BmeT110I CYCGRG 1 cut(s) 1388
BmgT120I GGNCC 4 cut(s) 150, 587, 1133, 1211
BmrFI CCNGG 4 cut(s) 591, 750, 809, 1350
BmsI GCATC 4 cut(s) 259, 273, 457, 613
BoxI GACNNNNGTC 1 cut(s) 132
BpiI GAAGAC 1 cut(s) 1270
BpmI CTGGAG 1 cut(s) 72
BpuEI CTTGAG 1 cut(s) 494
BpuMI CCSGG 1 cut(s) 809
Bsa29I ATCGAT 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 603
BsaJI CCNNGG 8 cut(s) 146, 611, 900, 916, 941, 1035, 1071, 1348
BsaWI WCCGGW 1 cut(s) 1251
Bsc4I CCNNNNNNNGG 1 cut(s) 1208
Bse1I ACTGG 3 cut(s) 55, 686, 1106
Bse3DI GCAATG 1 cut(s) 1365
Bse8I GATNNNNATC 1 cut(s) 603
BseAI TCCGGA 1 cut(s) 1251
BseBI CCWGG 3 cut(s) 591, 750, 1350
BseCI ATCGAT 1 cut(s) 488
BseDI CCNNGG 8 cut(s) 146, 611, 900, 916, 941, 1035, 1071, 1348
BseGI GGATG 3 cut(s) 555, 745, 1199
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 1 cut(s) 1208
BseMI GCAATG 1 cut(s) 1365
BseNI ACTGG 3 cut(s) 55, 686, 1106
BseRI GAGGAG 1 cut(s) 1055
BseXI GCAGC 6 cut(s) 86, 167, 331, 760, 1072, 1174
BsgI GTGCAG 1 cut(s) 93
BshVI ATCGAT 1 cut(s) 488
BsiHKAI GWGCWC 4 cut(s) 93, 109, 1070, 1434
BsiHKCI CYCGRG 1 cut(s) 1388
BsiSI CCGG 3 cut(s) 809, 1191, 1252
BslFI GGGAC 2 cut(s) 337, 744
BslI CCNNNNNNNGG 1 cut(s) 1208
BsmAI GTCTC 1 cut(s) 799
BsmFI GGGAC 2 cut(s) 337, 744
BsmI GAATGC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 1388
Bsp1286I GDGCHC 4 cut(s) 93, 109, 1070, 1434
Bsp13I TCCGGA 1 cut(s) 1251
Bsp1407I TGTACA 1 cut(s) 872
Bsp143I GATC 5 cut(s) 157, 457, 654, 909, 1121
Bsp19I CCATGG 1 cut(s) 941
BspACI CCGC 3 cut(s) 575, 1117, 1405
BspDI ATCGAT 1 cut(s) 488
BspEI TCCGGA 1 cut(s) 1251
BspMI ACCTGC 1 cut(s) 870
BspTI CTTAAG 1 cut(s) 1225
BsrDI GCAATG 1 cut(s) 1365
BsrGI TGTACA 1 cut(s) 872
BsrI ACTGG 3 cut(s) 55, 686, 1106
BssECI CCNNGG 8 cut(s) 146, 611, 900, 916, 941, 1035, 1071, 1348
BssMI GATC 5 cut(s) 157, 457, 654, 909, 1121
BssNAI GTATAC 1 cut(s) 555
BssT1I CCWWGG 7 cut(s) 146, 611, 900, 916, 941, 1035, 1071
Bst1107I GTATAC 1 cut(s) 555
Bst2UI CCWGG 3 cut(s) 591, 750, 1350
Bst4CI ACNGT 6 cut(s) 170, 242, 501, 803, 931, 1133
Bst6I CTCTTC 4 cut(s) 145, 653, 1247, 1412
BstAFI CTTAAG 1 cut(s) 1225
BstAUI TGTACA 1 cut(s) 872
BstC8I GCNNGC 1 cut(s) 109
BstDEI CTNAG 2 cut(s) 85, 201
BstDSI CCRYGG 1 cut(s) 941
BstEII GGTNACC 3 cut(s) 803, 960, 1197
BstF5I GGATG 3 cut(s) 555, 745, 1199
BstH2I RGCGCY 1 cut(s) 1386
BstHHI GCGC 2 cut(s) 895, 1385
BstKTI GATC 5 cut(s) 160, 460, 657, 912, 1124
BstMAI GTCTC 1 cut(s) 799
BstMBI GATC 5 cut(s) 157, 457, 654, 909, 1121
BstMWI GCNNNNNNNGC 3 cut(s) 117, 180, 1184
BstNI CCWGG 3 cut(s) 591, 750, 1350
BstNSI RCATGY 2 cut(s) 561, 1304
BstPAI GACNNNNGTC 1 cut(s) 132
BstPI GGTNACC 3 cut(s) 803, 960, 1197
BstSCI CCNGG 4 cut(s) 589, 748, 807, 1348
BstV1I GCAGC 6 cut(s) 86, 167, 331, 760, 1072, 1174
BstV2I GAAGAC 1 cut(s) 1270
BstX2I RGATCY 2 cut(s) 909, 1121
BstYI RGATCY 2 cut(s) 909, 1121
BstZ17I GTATAC 1 cut(s) 555
Bsu15I ATCGAT 1 cut(s) 488
BsuTUI ATCGAT 1 cut(s) 488
BtgI CCRYGG 1 cut(s) 941
BtsCI GGATG 3 cut(s) 555, 745, 1199
BtsI GCAGTG 1 cut(s) 1296
BtsIMutI CAGTG 4 cut(s) 48, 175, 927, 1296
BveI ACCTGC 1 cut(s) 870
Cac8I GCNNGC 1 cut(s) 109
CfoI GCGC 2 cut(s) 895, 1385
Cfr13I GGNCC 4 cut(s) 150, 587, 1133, 1211
ClaI ATCGAT 1 cut(s) 488
CseI GACGC 1 cut(s) 821
Csp6I GTAC 3 cut(s) 873, 884, 1295
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 6 cut(s) 356, 461, 558, 942, 1141, 1301
CviQI GTAC 3 cut(s) 873, 884, 1295
DdeI CTNAG 2 cut(s) 85, 201
DpnI GATC 5 cut(s) 159, 459, 656, 911, 1123
DpnII GATC 5 cut(s) 157, 457, 654, 909, 1121
DraI TTTAAA 1 cut(s) 352
Eam1104I CTCTTC 4 cut(s) 145, 653, 1247, 1412
EarI CTCTTC 4 cut(s) 145, 653, 1247, 1412
Ecl136II GAGCTC 2 cut(s) 91, 1068
Eco130I CCWWGG 7 cut(s) 146, 611, 900, 916, 941, 1035, 1071
Eco24I GRGCYC 2 cut(s) 93, 1070
Eco47I GGWCC 4 cut(s) 150, 587, 1133, 1211
Eco47III AGCGCT 1 cut(s) 1384
Eco53kI GAGCTC 2 cut(s) 91, 1068
Eco57I CTGAAG 1 cut(s) 1008
Eco88I CYCGRG 1 cut(s) 1388
Eco91I GGTNACC 3 cut(s) 803, 960, 1197
EcoICRI GAGCTC 2 cut(s) 91, 1068
EcoO109I RGGNCCY 1 cut(s) 1211
EcoO65I GGTNACC 3 cut(s) 803, 960, 1197
EcoRII CCWGG 3 cut(s) 589, 748, 1348
EcoT14I CCWWGG 7 cut(s) 146, 611, 900, 916, 941, 1035, 1071
EcoT22I ATGCAT 2 cut(s) 6, 472
EcoT38I GRGCYC 2 cut(s) 93, 1070
ErhI CCWWGG 7 cut(s) 146, 611, 900, 916, 941, 1035, 1071
FaeI CATG 6 cut(s) 359, 464, 561, 945, 1144, 1304
FalI AAGNNNNNCTT 2 cut(s) 1262, 1294
FaqI GGGAC 2 cut(s) 337, 744
FatI CATG 6 cut(s) 355, 460, 557, 941, 1140, 1300
FbaI TGATCA 1 cut(s) 457
FblI GTMKAC 4 cut(s) 523, 554, 993, 1344
Fnu4HI GCNGC 7 cut(s) 75, 181, 345, 576, 774, 1086, 1188
FokI GGATG 3 cut(s) 562, 752, 1206
FriOI GRGCYC 2 cut(s) 93, 1070
Fsp4HI GCNGC 7 cut(s) 75, 181, 345, 576, 774, 1086, 1188
FspBI CTAG 2 cut(s) 612, 707
GlaI GCGC 2 cut(s) 894, 1384
GluI GCNGC 7 cut(s) 75, 181, 345, 576, 774, 1086, 1188
GsuI CTGGAG 1 cut(s) 72
HaeII RGCGCY 1 cut(s) 1386
HapII CCGG 3 cut(s) 809, 1191, 1252
HgaI GACGC 1 cut(s) 821
HhaI GCGC 2 cut(s) 895, 1385
Hin1II CATG 6 cut(s) 359, 464, 561, 945, 1144, 1304
Hin6I GCGC 2 cut(s) 893, 1383
HinP1I GCGC 2 cut(s) 893, 1383
HincII GTYRAC 4 cut(s) 313, 524, 782, 1345
HindII GTYRAC 4 cut(s) 313, 524, 782, 1345
HinfI GANTC 5 cut(s) 415, 565, 732, 1314, 1341
HpaI GTTAAC 1 cut(s) 313
HpaII CCGG 3 cut(s) 809, 1191, 1252
HphI GGTGA 3 cut(s) 203, 671, 823
Hpy166II GTNNAC 9 cut(s) 313, 524, 555, 662, 782, 873, 994, 1146, 1345
Hpy188I TCNGA 3 cut(s) 154, 679, 799
Hpy188III TCNNGA 6 cut(s) 452, 511, 652, 1063, 1252, 1261
Hpy8I GTNNAC 9 cut(s) 313, 524, 555, 662, 782, 873, 994, 1146, 1345
Hpy99I CGWCG 2 cut(s) 71, 102
HpyAV CCTTC 8 cut(s) 14, 23, 49, 271, 425, 752, 1070, 1304
HpyCH4III ACNGT 6 cut(s) 170, 242, 501, 803, 931, 1133
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 180, 1184
HpyF3I CTNAG 2 cut(s) 85, 201
Hsp92II CATG 6 cut(s) 359, 464, 561, 945, 1144, 1304
HspAI GCGC 2 cut(s) 893, 1383
Kpn2I TCCGGA 1 cut(s) 1251
Ksp22I TGATCA 1 cut(s) 457
KspAI GTTAAC 1 cut(s) 313
Kzo9I GATC 5 cut(s) 157, 457, 654, 909, 1121
LmnI GCTCC 3 cut(s) 233, 1042, 1073
Lsp1109I GCAGC 6 cut(s) 86, 167, 331, 760, 1072, 1174
LweI GCATC 4 cut(s) 259, 273, 457, 613
MaeI CTAG 2 cut(s) 612, 707
MalI GATC 5 cut(s) 159, 459, 656, 911, 1123
MboI GATC 5 cut(s) 157, 457, 654, 909, 1121
MboII GAAGA 9 cut(s) 132, 599, 608, 640, 904, 971, 1234, 1270, 1429
MflI RGATCY 2 cut(s) 909, 1121
MhlI GDGCHC 4 cut(s) 93, 109, 1070, 1434
MluCI AATT 1 cut(s) 369
MlyI GAGTC 2 cut(s) 726, 1350
MmeI TCCRAC 1 cut(s) 1160
MnlI CCTC 7 cut(s) 47, 140, 301, 459, 533, 1033, 1250
Mph1103I ATGCAT 2 cut(s) 6, 472
MroI TCCGGA 1 cut(s) 1251
MseI TTAA 6 cut(s) 312, 351, 531, 1029, 1226, 1441
MslI CAYNNNNRTG 3 cut(s) 9, 41, 465
MspA1I CMGCKG 1 cut(s) 575
MspCI CTTAAG 1 cut(s) 1225
MspI CCGG 3 cut(s) 809, 1191, 1252
MspR9I CCNGG 4 cut(s) 591, 750, 809, 1350
MssI GTTTAAAC 1 cut(s) 352
Mva1269I GAATGC 1 cut(s) 183
MvaI CCWGG 3 cut(s) 591, 750, 1350
MwoI GCNNNNNNNGC 3 cut(s) 117, 180, 1184
NciI CCSGG 1 cut(s) 809
NcoI CCATGG 1 cut(s) 941
NdeII GATC 5 cut(s) 157, 457, 654, 909, 1121
NlaIII CATG 6 cut(s) 359, 464, 561, 945, 1144, 1304
NmuCI GTSAC 3 cut(s) 46, 134, 196
NsiI ATGCAT 2 cut(s) 6, 472
NspI RCATGY 2 cut(s) 561, 1304
OliI CACNNNNGTG 1 cut(s) 41
PciI ACATGT 2 cut(s) 557, 1300
PcsI WCGNNNNNNNCGW 1 cut(s) 295
PctI GAATGC 1 cut(s) 183
PfeI GAWTC 3 cut(s) 415, 565, 1314
PkrI GCNGC 7 cut(s) 76, 182, 346, 577, 775, 1087, 1189
PleI GAGTC 2 cut(s) 726, 1349
PmeI GTTTAAAC 1 cut(s) 352
PpsI GAGTC 2 cut(s) 726, 1349
PpuMI RGGWCCY 1 cut(s) 1211
PscI ACATGT 2 cut(s) 557, 1300
PshAI GACNNNNGTC 1 cut(s) 132
Psp124BI GAGCTC 2 cut(s) 93, 1070
Psp5II RGGWCCY 1 cut(s) 1211
Psp6I CCWGG 3 cut(s) 589, 748, 1348
PspEI GGTNACC 3 cut(s) 803, 960, 1197
PspGI CCWGG 3 cut(s) 589, 748, 1348
PspPI GGNCC 4 cut(s) 150, 587, 1133, 1211
PspPPI RGGWCCY 1 cut(s) 1211
PsuI RGATCY 2 cut(s) 909, 1121
RsaI GTAC 3 cut(s) 874, 885, 1296
RsaNI GTAC 3 cut(s) 873, 884, 1295
RseI CAYNNNNRTG 3 cut(s) 9, 41, 465
SacI GAGCTC 2 cut(s) 93, 1070
SalI GTCGAC 2 cut(s) 522, 1343
SaqAI TTAA 6 cut(s) 312, 351, 531, 1029, 1226, 1441
SatI GCNGC 7 cut(s) 75, 181, 345, 576, 774, 1086, 1188
Sau3AI GATC 5 cut(s) 157, 457, 654, 909, 1121
Sau96I GGNCC 4 cut(s) 150, 587, 1133, 1211
SchI GAGTC 2 cut(s) 726, 1350
ScrFI CCNGG 4 cut(s) 591, 750, 809, 1350
SduI GDGCHC 4 cut(s) 93, 109, 1070, 1434
SfaNI GCATC 4 cut(s) 259, 273, 457, 613
SinI GGWCC 4 cut(s) 150, 587, 1133, 1211
SmiMI CAYNNNNRTG 3 cut(s) 9, 41, 465
SmlI CTYRAG 2 cut(s) 509, 1225
SmoI CTYRAG 2 cut(s) 509, 1225
Sse9I AATT 1 cut(s) 369
SsiI CCGC 3 cut(s) 575, 1117, 1405
SspMI CTAG 2 cut(s) 612, 707
SstI GAGCTC 2 cut(s) 93, 1070
StyD4I CCNGG 4 cut(s) 589, 748, 807, 1348
StyI CCWWGG 7 cut(s) 146, 611, 900, 916, 941, 1035, 1071
TaaI ACNGT 6 cut(s) 170, 242, 501, 803, 931, 1133
TaqI TCGA 6 cut(s) 27, 478, 488, 523, 693, 1344
TasI AATT 1 cut(s) 369
TatI WGTACW 1 cut(s) 872
TauI GCSGC 1 cut(s) 578
TfiI GAWTC 3 cut(s) 415, 565, 1314
Tru1I TTAA 6 cut(s) 312, 351, 531, 1029, 1226, 1441
Tru9I TTAA 6 cut(s) 312, 351, 531, 1029, 1226, 1441
TscAI CASTG 4 cut(s) 55, 175, 934, 1296
TseFI GTSAC 3 cut(s) 46, 134, 196
TseI GCWGC 6 cut(s) 74, 180, 344, 773, 1085, 1187
Tsp45I GTSAC 3 cut(s) 46, 134, 196
TspDTI ATGAA 3 cut(s) 33, 444, 1270
TspGWI ACGGA 2 cut(s) 122, 562
TspRI CASTG 4 cut(s) 55, 175, 934, 1296
Vha464I CTTAAG 1 cut(s) 1225
VpaK11BI GGWCC 4 cut(s) 150, 587, 1133, 1211
XceI RCATGY 2 cut(s) 561, 1304
XcmI CCANNNNNNNNNTGG 1 cut(s) 1078
XmaJI CCTAGG 1 cut(s) 611
XmiI GTMKAC 4 cut(s) 523, 554, 993, 1344
XspI CTAG 2 cut(s) 612, 707
Zsp2I ATGCAT 2 cut(s) 6, 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.