Prupe.5G005700_v2.0.a1

Short-chain dehydrogenase reductase family 42E member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0012481
Physical Location & Seq
Reverse (-)
673356 .. 677659
4304 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G005700.1

Sequence Viewer

Length: 1485 bp
ATGCACTTAAGCGAAAATGAAGGCATAGAAGGCAACACTTTTGTGGTGACGGGTGGGTTGGGGTTTGTGGGCTCCGCACTCTGCTTGGAGCTGCTTCGAAGAGGGGCTCACCAAGTCCGAGCCTTTGACCTCCGAACGACCTCCCCTTGGTCCGCCAATCTCCTCCAACACGGCGTCCACTGCGTCCAAGGGGATGTTACCCATAAAAAAGATGTTGAAAAGGCTCTGCGCGGAGCAGACTGTGTGTTCCACCTTGCTTCATATGGCATGTCAGGGAAAGAAATGCTGCAGTTTGGTCGTGTTGACGAGGTCAATATAAATGGAACCTGTCATATATTAGACGCTTGTCTCGAGTTTGGGATCCAAAGGCTTGTTTATGTGAGCACGTACAATGTTGTGTTTGGGGGAGAGGAGATTGTCAATGGGAATGAGGCCTTGCGCTACTTCCCCATTGATAGCCATGTCGACCCGTATGGCCGTAGTAAATCAATTGCCGAGCAGTTGGTCCTCAAGTACAATGCTCGTCCCTTTAGGAAGAAGAGTGGGAAGTGCCTTTACACCTGTGCAGTTCGTCCAGCTGCTATCTATGGTCCAGGTGAAGAAAGGCACCTTCCGAGGATAGTGTCTCTGGCAAAGTTAGGCCTAGTTCCATTCAAAATTGGCGAAGCAAATGTGAAAACAGATTGGATTTATGTCGATAACCTAGTACTTGCACTGGTAATGGCAAGCATGGGACTTTTGGATGACATTCCCGGGAGAGATAAAGAAAGACATCCCATAGCTGCTGGTCAGCCATATTTTGTATCCGATGGGTCTCCAGTCAACACCTTTGAGTTCATCCGACCTCTGCTGAGAAGCTTGGATTATGACCTACCAAAGGCCTCCTTGTCTGTACCTCGTGCTCTTTCTTTAGGAAAGGTTTTTTGGGCAGTCTATACTATCTTGTATCCATGGTTAAATCGATGGTGGCTTCCTCAGCCATTGATCCTTCCTGCTGAAGTATACAAGGTTGGTGTTACCCATTACTTCTCTTTCCTTAAAGCAAAGGAGGAGCTTGGCTATGTCCCAATGGTGACTCCTCGAGAGGGAATGGCTGCTACGATCTCATACTGGCAGGAGAGGAAAAGGAAAACTCTGGATGGGCCTACAATATATGCATGGCTATTTTGTGTGCTTGGAATGGCCACGCTATTCTGTGCTGCTTATCTGCCAGACATTGGACCTGTGCCACTCTTCAGAGCTATTAGCCTCTTCTTTCTCCGGTCAATGAGAGTAGTTAGGACAACGTTTCTCCTAGCTGCAGCAGCACATATTGGAGAGGCTGTATACGCGTGGAAGCTGGCAAAAAGGGTGGACCCTGCTAACTCAAGAGGATGGTTTTGGCAGACCTTGGCTCTTGGGTTTTTTTCATTACGTTTTCTGTTGAAGAGAGCACGGAAGGCAAGAGCTCCTCTGCATGCCTGTAGTGTTCGTAGAATTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

495

Amino Acids

55.45

Weight (kDa)

9.4

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33630
fragaria_vesca FvH4_5g26130 FvH4_5g26130
malus_domestica MD06G1004300.v1.1
prunus_persica Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1
pyrus_communis pycom06g00410
rosa_chinensis RchiOBHm_Chr7g0216661
rosa_laevigata RLG00000002552
rosa_multiflora Rmu_sc0029435.1_g000001 Rmu_ssc0000117.1_g000009
rosa_roxburghii Rroxscaffold_3G00243030
rosa_rugosa Rorug07G0161500
rosa_samantha Rh7AG301300 Rh7BG292800 Rh7CG319900 Rh7DG301900
rosa_wichuraiana Rw7G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1483
AccB1I GGYRCC 1 cut(s) 606
AccB7I CCANNNNNTGG 1 cut(s) 1217
AccI GTMKAC 3 cut(s) 465, 1002, 1326
AccII CGCG 2 cut(s) 231, 1331
AciI CCGC 3 cut(s) 75, 153, 231
AclI AACGTT 1 cut(s) 1286
AclWI GGATC 3 cut(s) 355, 368, 979
AcoI YGGCCR 2 cut(s) 475, 1182
AcsI RAATTY 1 cut(s) 1476
AcuI CTGAAG 2 cut(s) 1017, 1219
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 4 cut(s) 389, 515, 708, 894
AfiI CCNNNNNNNGG 4 cut(s) 147, 877, 1085, 1217
AflII CTTAAG 1 cut(s) 7
AflIII ACRYGT 1 cut(s) 1329
AgsI TTSAA 3 cut(s) 218, 655, 1426
AjnI CCWGG 1 cut(s) 592
AjuI GAANNNNNNNTTGG 2 cut(s) 907, 939
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 9 cut(s) 91, 578, 782, 858, 1054, 1241, 1298, 1339, 1448
AluI AGCT 9 cut(s) 91, 578, 782, 858, 1054, 1241, 1298, 1339, 1448
Alw21I GWGCWC 4 cut(s) 386, 904, 1435, 1450
Alw26I GTCTC 3 cut(s) 353, 630, 819
AlwI GGATC 3 cut(s) 355, 368, 979
Ama87I CYCGRG 3 cut(s) 350, 752, 1080
AoxI GGCC 6 cut(s) 432, 475, 640, 879, 1142, 1182
ApeKI GCWGC 9 cut(s) 91, 286, 578, 782, 1094, 1199, 1298, 1301, 1304
ApoI RAATTY 1 cut(s) 1476
AspLEI GCGC 2 cut(s) 231, 441
AspS9I GGNCC 6 cut(s) 150, 505, 590, 1142, 1220, 1354
AsuC2I CCSGG 2 cut(s) 753, 754
AsuHPI GGTGA 4 cut(s) 58, 101, 608, 1084
AsuII TTCGAA 1 cut(s) 97
AvaI CYCGRG 3 cut(s) 350, 752, 1080
AvaII GGWCC 5 cut(s) 150, 505, 590, 1220, 1354
BalI TGGCCA 1 cut(s) 1184
BamHI GGATCC 1 cut(s) 360
BanI GGYRCC 1 cut(s) 606
BanII GRGCYC 3 cut(s) 74, 109, 1450
BarI GAAGNNNNNNTAC 2 cut(s) 539, 571
BauI CACGAG 1 cut(s) 897
Bbv12I GWGCWC 4 cut(s) 386, 904, 1435, 1450
BbvCI CCTCAGC 1 cut(s) 975
BbvI GCAGC 9 cut(s) 78, 273, 565, 769, 1081, 1186, 1285, 1313, 1316
BccI CCATC 4 cut(s) 803, 957, 1133, 1368
BceAI ACGGC 2 cut(s) 187, 462
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 1 cut(s) 594
BciVI GTATCC 2 cut(s) 814, 957
BcnI CCSGG 2 cut(s) 753, 754
BcoDI GTCTC 3 cut(s) 353, 630, 819
BfaI CTAG 3 cut(s) 644, 704, 1295
BfmI CTRYAG 3 cut(s) 287, 1299, 1462
BfrI CTTAAG 1 cut(s) 7
BfuI GTATCC 2 cut(s) 814, 957
BisI GCNGC 9 cut(s) 92, 287, 579, 783, 1095, 1200, 1299, 1302, 1305
BlsI GCNGC 9 cut(s) 93, 288, 580, 784, 1096, 1201, 1300, 1303, 1306
BmcAI AGTACT 1 cut(s) 708
Bme1390I CCNGG 3 cut(s) 594, 753, 754
Bme18I GGWCC 5 cut(s) 150, 505, 590, 1220, 1354
BmeT110I CYCGRG 3 cut(s) 350, 752, 1080
BmgT120I GGNCC 6 cut(s) 150, 505, 590, 1142, 1220, 1354
BmiI GGNNCC 5 cut(s) 73, 325, 362, 608, 1356
BmrFI CCNGG 3 cut(s) 594, 753, 754
BoxI GACNNNNGTC 1 cut(s) 345
BpmI CTGGAG 1 cut(s) 801
Bpu10I CCTNAGC 1 cut(s) 975
Bpu14I TTCGAA 1 cut(s) 97
BpuEI CTTGAG 2 cut(s) 494, 1351
BpuMI CCSGG 2 cut(s) 753, 754
Bsa29I ATCGAT 1 cut(s) 961
BsaAI YACGTR 1 cut(s) 387
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 819
BsaJI CCNNGG 6 cut(s) 146, 187, 614, 752, 950, 1389
BsaWI WCCGGW 1 cut(s) 1260
BsaXI ACNNNNNCTCC 2 cut(s) 1308, 1338
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 877, 1085, 1217
Bse1I ACTGG 3 cut(s) 720, 818, 1115
BseBI CCWGG 1 cut(s) 594
BseCI ATCGAT 1 cut(s) 961
BseDI CCNNGG 6 cut(s) 146, 187, 614, 752, 950, 1389
BseGI GGATG 6 cut(s) 199, 748, 772, 837, 1144, 1379
BseLI CCNNNNNNNGG 4 cut(s) 147, 877, 1085, 1217
BseMII CTCAG 2 cut(s) 842, 989
BseNI ACTGG 3 cut(s) 720, 818, 1115
BseRI GAGGAG 5 cut(s) 152, 425, 1064, 1068, 1440
BseXI GCAGC 9 cut(s) 78, 273, 565, 769, 1081, 1186, 1285, 1313, 1316
BsgI GTGCAG 1 cut(s) 585
Bsh1236I CGCG 2 cut(s) 231, 1331
BshFI GGCC 6 cut(s) 434, 477, 642, 881, 1144, 1184
BshNI GGYRCC 1 cut(s) 606
BshVI ATCGAT 1 cut(s) 961
BsiHKAI GWGCWC 4 cut(s) 386, 904, 1435, 1450
BsiHKCI CYCGRG 3 cut(s) 350, 752, 1080
BsiSI CCGG 2 cut(s) 753, 1261
BslFI GGGAC 3 cut(s) 510, 747, 1049
BslI CCNNNNNNNGG 4 cut(s) 147, 877, 1085, 1217
BsmAI GTCTC 3 cut(s) 353, 630, 819
BsmFI GGGAC 3 cut(s) 510, 747, 1049
BsnI GGCC 6 cut(s) 434, 477, 642, 881, 1144, 1184
Bso31I GGTCTC 1 cut(s) 819
BsoBI CYCGRG 3 cut(s) 350, 752, 1080
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 6 cut(s) 74, 109, 386, 904, 1435, 1450
Bsp143I GATC 3 cut(s) 360, 984, 1101
Bsp19I CCATGG 1 cut(s) 950
BspACI CCGC 3 cut(s) 75, 153, 231
BspANI GGCC 6 cut(s) 434, 477, 642, 881, 1144, 1184
BspCNI CTCAG 2 cut(s) 843, 988
BspDI ATCGAT 1 cut(s) 961
BspFNI CGCG 2 cut(s) 231, 1331
BspLI GGNNCC 5 cut(s) 73, 325, 362, 608, 1356
BspMAI CTGCAG 2 cut(s) 291, 1303
BspPI GGATC 3 cut(s) 355, 368, 979
BspT104I TTCGAA 1 cut(s) 97
BspT107I GGYRCC 1 cut(s) 606
BspTI CTTAAG 1 cut(s) 7
BspTNI GGTCTC 1 cut(s) 819
BsrI ACTGG 3 cut(s) 720, 818, 1115
BssECI CCNNGG 6 cut(s) 146, 187, 614, 752, 950, 1389
BssMI GATC 3 cut(s) 360, 984, 1101
BssNAI GTATAC 2 cut(s) 1003, 1327
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 897
BssT1I CCWWGG 4 cut(s) 146, 187, 950, 1389
Bst1107I GTATAC 2 cut(s) 1003, 1327
Bst2BI CACGAG 1 cut(s) 897
Bst2UI CCWGG 1 cut(s) 594
Bst4CI ACNGT 1 cut(s) 242
Bst6I CTCTTC 5 cut(s) 94, 533, 1238, 1256, 1421
BstACI GRCGYC 1 cut(s) 174
BstAFI CTTAAG 1 cut(s) 7
BstBAI YACGTR 1 cut(s) 387
BstBI TTCGAA 1 cut(s) 97
BstC8I GCNNGC 3 cut(s) 727, 1341, 1458
BstDEI CTNAG 2 cut(s) 851, 975
BstDSI CCRYGG 1 cut(s) 950
BstENI CCTNNNNNAGG 1 cut(s) 875
BstF5I GGATG 6 cut(s) 199, 748, 772, 837, 1144, 1379
BstFNI CGCG 2 cut(s) 231, 1331
BstHHI GCGC 2 cut(s) 231, 441
BstKTI GATC 3 cut(s) 363, 987, 1104
BstMAI GTCTC 3 cut(s) 353, 630, 819
BstMBI GATC 3 cut(s) 360, 984, 1101
BstMWI GCNNNNNNNGC 6 cut(s) 30, 180, 976, 1304, 1328, 1439
BstNI CCWGG 1 cut(s) 594
BstNSI RCATGY 2 cut(s) 271, 1460
BstPAI GACNNNNGTC 1 cut(s) 345
BstSCI CCNGG 3 cut(s) 592, 751, 752
BstSFI CTRYAG 3 cut(s) 287, 1299, 1462
BstUI CGCG 2 cut(s) 231, 1331
BstV1I GCAGC 9 cut(s) 78, 273, 565, 769, 1081, 1186, 1285, 1313, 1316
BstX2I RGATCY 1 cut(s) 360
BstYI RGATCY 1 cut(s) 360
BstZ17I GTATAC 2 cut(s) 1003, 1327
Bsu15I ATCGAT 1 cut(s) 961
BsuI GTATCC 2 cut(s) 814, 957
BsuRI GGCC 6 cut(s) 434, 477, 642, 881, 1144, 1184
BsuTUI ATCGAT 1 cut(s) 961
BtgI CCRYGG 1 cut(s) 950
BtsCI GGATG 6 cut(s) 199, 748, 772, 837, 1144, 1379
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 2 cut(s) 178, 713
Cac8I GCNNGC 3 cut(s) 727, 1341, 1458
CfoI GCGC 2 cut(s) 231, 441
Cfr13I GGNCC 6 cut(s) 150, 505, 590, 1142, 1220, 1354
Cfr9I CCCGGG 1 cut(s) 752
ClaI ATCGAT 1 cut(s) 961
CseI GACGC 3 cut(s) 163, 172, 350
Csp6I GTAC 4 cut(s) 388, 514, 707, 893
CspCI CAANNNNNGTGG 2 cut(s) 1332, 1367
CviAII CATG 6 cut(s) 268, 461, 730, 951, 1158, 1457
CviQI GTAC 4 cut(s) 388, 514, 707, 893
DdeI CTNAG 2 cut(s) 851, 975
DpnI GATC 3 cut(s) 362, 986, 1103
DpnII GATC 3 cut(s) 360, 984, 1101
EaeI YGGCCR 2 cut(s) 475, 1182
Eam1104I CTCTTC 5 cut(s) 94, 533, 1238, 1256, 1421
EarI CTCTTC 5 cut(s) 94, 533, 1238, 1256, 1421
EciI GGCGGA 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 1448
Eco130I CCWWGG 4 cut(s) 146, 187, 950, 1389
Eco147I AGGCCT 3 cut(s) 434, 642, 881
Eco24I GRGCYC 3 cut(s) 74, 109, 1450
Eco31I GGTCTC 1 cut(s) 819
Eco47I GGWCC 5 cut(s) 150, 505, 590, 1220, 1354
Eco53kI GAGCTC 1 cut(s) 1448
Eco57I CTGAAG 2 cut(s) 1017, 1219
Eco88I CYCGRG 3 cut(s) 350, 752, 1080
EcoICRI GAGCTC 1 cut(s) 1448
EcoNI CCTNNNNNAGG 1 cut(s) 875
EcoRII CCWGG 1 cut(s) 592
EcoT14I CCWWGG 4 cut(s) 146, 187, 950, 1389
EcoT22I ATGCAT 1 cut(s) 1159
EcoT38I GRGCYC 3 cut(s) 74, 109, 1450
ErhI CCWWGG 4 cut(s) 146, 187, 950, 1389
FaeI CATG 6 cut(s) 271, 464, 733, 954, 1161, 1460
FalI AAGNNNNNCTT 2 cut(s) 869, 901
FaqI GGGAC 3 cut(s) 510, 747, 1049
FatI CATG 6 cut(s) 267, 460, 729, 950, 1157, 1456
FauNDI CATATG 1 cut(s) 262
FblI GTMKAC 3 cut(s) 465, 1002, 1326
Fnu4HI GCNGC 9 cut(s) 92, 287, 579, 783, 1095, 1200, 1299, 1302, 1305
FokI GGATG 6 cut(s) 206, 755, 759, 824, 1151, 1386
FriOI GRGCYC 3 cut(s) 74, 109, 1450
Fsp4HI GCNGC 9 cut(s) 92, 287, 579, 783, 1095, 1200, 1299, 1302, 1305
FspBI CTAG 3 cut(s) 644, 704, 1295
GlaI GCGC 2 cut(s) 230, 440
GluI GCNGC 9 cut(s) 92, 287, 579, 783, 1095, 1200, 1299, 1302, 1305
GsuI CTGGAG 1 cut(s) 801
HaeIII GGCC 6 cut(s) 434, 477, 642, 881, 1144, 1184
HapII CCGG 2 cut(s) 753, 1261
HgaI GACGC 3 cut(s) 163, 172, 350
HhaI GCGC 2 cut(s) 231, 441
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 6 cut(s) 271, 464, 733, 954, 1161, 1460
Hin6I GCGC 2 cut(s) 229, 439
HinP1I GCGC 2 cut(s) 229, 439
HincII GTYRAC 3 cut(s) 304, 466, 823
HindII GTYRAC 3 cut(s) 304, 466, 823
HindIII AAGCTT 1 cut(s) 856
HinfI GANTC 1 cut(s) 1075
HpaII CCGG 2 cut(s) 753, 1261
HphI GGTGA 4 cut(s) 58, 101, 608, 1084
Hpy166II GTNNAC 7 cut(s) 178, 304, 466, 823, 1003, 1327, 1354
Hpy188I TCNGA 6 cut(s) 119, 134, 615, 808, 842, 1238
Hpy188III TCNNGA 4 cut(s) 350, 1082, 1136, 1368
Hpy8I GTNNAC 7 cut(s) 178, 304, 466, 823, 1003, 1327, 1354
HpyAV CCTTC 5 cut(s) 14, 23, 620, 998, 1432
HpyCH4III ACNGT 1 cut(s) 242
HpyCH4IV ACGT 3 cut(s) 386, 1286, 1414
HpyCH4V TGCA 7 cut(s) 4, 289, 566, 713, 1157, 1301, 1456
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 180, 976, 1304, 1328, 1439
HpyF3I CTNAG 2 cut(s) 851, 975
HpySE526I ACGT 3 cut(s) 386, 1286, 1414
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 6 cut(s) 271, 464, 733, 954, 1161, 1460
HspAI GCGC 2 cut(s) 229, 439
Kzo9I GATC 3 cut(s) 360, 984, 1101
LmnI GCTCC 5 cut(s) 77, 88, 233, 1051, 1453
Lsp1109I GCAGC 9 cut(s) 78, 273, 565, 769, 1081, 1186, 1285, 1313, 1316
MaeI CTAG 3 cut(s) 644, 704, 1295
MaeII ACGT 3 cut(s) 386, 1286, 1414
MaeIII GTNAC 4 cut(s) 46, 196, 1015, 1072
MalI GATC 3 cut(s) 362, 986, 1103
MboI GATC 3 cut(s) 360, 984, 1101
MboII GAAGA 7 cut(s) 111, 547, 550, 611, 1225, 1243, 1438
MfeI CAATTG 1 cut(s) 489
MflI RGATCY 1 cut(s) 360
MhlI GDGCHC 6 cut(s) 74, 109, 386, 904, 1435, 1450
MlsI TGGCCA 1 cut(s) 1184
MluCI AATT 3 cut(s) 489, 657, 1476
MluI ACGCGT 1 cut(s) 1329
MluNI TGGCCA 1 cut(s) 1184
MlyI GAGTC 1 cut(s) 1069
MmeI TCCRAC 2 cut(s) 190, 865
Mox20I TGGCCA 1 cut(s) 1184
Mph1103I ATGCAT 1 cut(s) 1159
MscI TGGCCA 1 cut(s) 1184
MseI TTAA 3 cut(s) 8, 956, 1038
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 1184
MspA1I CMGCKG 1 cut(s) 578
MspCI CTTAAG 1 cut(s) 7
MspI CCGG 2 cut(s) 753, 1261
MspR9I CCNGG 3 cut(s) 594, 753, 754
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 594
MvnI CGCG 2 cut(s) 231, 1331
MwoI GCNNNNNNNGC 6 cut(s) 30, 180, 976, 1304, 1328, 1439
NciI CCSGG 2 cut(s) 753, 754
NcoI CCATGG 1 cut(s) 950
NdeI CATATG 1 cut(s) 262
NdeII GATC 3 cut(s) 360, 984, 1101
NlaIII CATG 6 cut(s) 271, 464, 733, 954, 1161, 1460
NlaIV GGNNCC 5 cut(s) 73, 325, 362, 608, 1356
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 2 cut(s) 46, 1072
NsiI ATGCAT 1 cut(s) 1159
NspI RCATGY 2 cut(s) 271, 1460
NspV TTCGAA 1 cut(s) 97
OliI CACNNNNGTG 1 cut(s) 41
PaeI GCATGC 1 cut(s) 1460
PaeR7I CTCGAG 2 cut(s) 350, 1080
PceI AGGCCT 3 cut(s) 434, 642, 881
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PflFI GACNNNGTC 1 cut(s) 308
PflMI CCANNNNNTGG 1 cut(s) 1217
PkrI GCNGC 9 cut(s) 93, 288, 580, 784, 1096, 1201, 1300, 1303, 1306
PleI GAGTC 1 cut(s) 1069
PpsI GAGTC 1 cut(s) 1069
Ppu21I YACGTR 1 cut(s) 387
PshAI GACNNNNGTC 1 cut(s) 345
PsiI TTATAA 1 cut(s) 1483
Psp124BI GAGCTC 1 cut(s) 1450
Psp1406I AACGTT 1 cut(s) 1286
Psp6I CCWGG 1 cut(s) 592
PspGI CCWGG 1 cut(s) 592
PspN4I GGNNCC 5 cut(s) 73, 325, 362, 608, 1356
PspPI GGNCC 6 cut(s) 150, 505, 590, 1142, 1220, 1354
PstI CTGCAG 2 cut(s) 291, 1303
PsuI RGATCY 1 cut(s) 360
PsyI GACNNNGTC 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 4 cut(s) 389, 515, 708, 894
RsaNI GTAC 4 cut(s) 388, 514, 707, 893
RseI CAYNNNNRTG 1 cut(s) 41
SacI GAGCTC 1 cut(s) 1450
SalI GTCGAC 1 cut(s) 464
SaqAI TTAA 3 cut(s) 8, 956, 1038
SatI GCNGC 9 cut(s) 92, 287, 579, 783, 1095, 1200, 1299, 1302, 1305
Sau3AI GATC 3 cut(s) 360, 984, 1101
Sau96I GGNCC 6 cut(s) 150, 505, 590, 1142, 1220, 1354
ScaI AGTACT 1 cut(s) 708
SchI GAGTC 1 cut(s) 1069
ScrFI CCNGG 3 cut(s) 594, 753, 754
SduI GDGCHC 6 cut(s) 74, 109, 386, 904, 1435, 1450
SfcI CTRYAG 3 cut(s) 287, 1299, 1462
Sfr274I CTCGAG 2 cut(s) 350, 1080
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 5 cut(s) 150, 505, 590, 1220, 1354
SlaI CTCGAG 2 cut(s) 350, 1080
SmaI CCCGGG 1 cut(s) 754
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 5 cut(s) 7, 350, 509, 1080, 1366
SmoI CTYRAG 5 cut(s) 7, 350, 509, 1080, 1366
SphI GCATGC 1 cut(s) 1460
Sse9I AATT 3 cut(s) 489, 657, 1476
SseBI AGGCCT 3 cut(s) 434, 642, 881
SsiI CCGC 3 cut(s) 75, 153, 231
SspMI CTAG 3 cut(s) 644, 704, 1295
SstI GAGCTC 1 cut(s) 1450
StuI AGGCCT 3 cut(s) 434, 642, 881
StyD4I CCNGG 3 cut(s) 592, 751, 752
StyI CCWWGG 4 cut(s) 146, 187, 950, 1389
TaaI ACNGT 1 cut(s) 242
TaiI ACGT 3 cut(s) 389, 1289, 1417
TaqI TCGA 6 cut(s) 97, 351, 465, 696, 961, 1081
TasI AATT 3 cut(s) 489, 657, 1476
TatI WGTACW 2 cut(s) 513, 706
Tru1I TTAA 3 cut(s) 8, 956, 1038
Tru9I TTAA 3 cut(s) 8, 956, 1038
TscAI CASTG 2 cut(s) 185, 720
TseFI GTSAC 2 cut(s) 46, 1072
TseI GCWGC 9 cut(s) 91, 286, 578, 782, 1094, 1199, 1298, 1301, 1304
Tsp45I GTSAC 2 cut(s) 46, 1072
TspDTI ATGAA 4 cut(s) 33, 249, 826, 1398
TspGWI ACGGA 1 cut(s) 1450
TspMI CCCGGG 1 cut(s) 752
TspRI CASTG 2 cut(s) 185, 720
Tth111I GACNNNGTC 1 cut(s) 308
Van91I CCANNNNNTGG 1 cut(s) 1217
Vha464I CTTAAG 1 cut(s) 7
VpaK11BI GGWCC 5 cut(s) 150, 505, 590, 1220, 1354
XagI CCTNNNNNAGG 1 cut(s) 875
XapI RAATTY 1 cut(s) 1476
XceI RCATGY 2 cut(s) 271, 1460
XhoI CTCGAG 2 cut(s) 350, 1080
XmaI CCCGGG 1 cut(s) 752
XmiI GTMKAC 3 cut(s) 465, 1002, 1326
XspI CTAG 3 cut(s) 644, 704, 1295
ZrmI AGTACT 1 cut(s) 708
Zsp2I ATGCAT 1 cut(s) 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.