Rroxscaffold_3G00243030

Short-chain dehydrogenase reductase family 42E member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0012481
Physical Location & Seq
Reverse (-)
35566231 .. 35568887
2657 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00243030.1

Sequence Viewer

Length: 1446 bp
ATGCACCTGAGCGAAAATGAAGGCATCGACGGCAGCACTTTCGTGGTCACCGGCGGTTTGGGCTTCGTGGGCTCCGCACTCTGCCTGGAGCTTATCCGACGAGGAGCTCGTCAAGTCCGAGCCTTTGACCGCCGAACCTCCTCCCCTTGGTCCCCCCATCTCCTCAACCAAGGGGTCAACTGCATCCAAGGGGATGTTGTTAACAAAAAGGATGTGGAGAAGGCTCTGCGGGGGGCAGATTGTGTGTTCCACCTAGCCTCGTACGGAATGTCGGGAAAGGAAATGCTGCAATTCGGGCGTGTTGATGAGGTTAATATCAATGGAACTTGTCATATATTGGATACTTGTCTCGATTTCGGGGTCGAAAGGCTTGTGTATGTGAGCACATATAATGTTGTGTTTGGGGGAAAGGAGATTGTGAGTGGGAATGAGGCCCTAAGCTATTTTCCTATTGATAGTCATGAGGATCCATACGGCAGAAGTAAATCCATTGCTGAGCAGTTGGTTCTTAAGTACAATGCTCGTCCTTTCAGGACGAAGAATGGGAAGTGTCTGTATACCTGTGCGGTTCGTCCAGCTGCTATCTATGGACCTGGTGAAGAAAGGCACCTTCCGAGGATAGTGTCCCTAGCAAAGTTAGGCTTAATTCCGTTCAAAATTGGCGAAGCAAATGTGAAAACAGACTGGATTTATGTGGATAATCTAGTGCTCGCTCTCATATTGGCGAGCATGGGACTTTTGCATGACATTCCTGGGAGAGAGAAAGAAAGACAACCCATAGCTGCTGGCCAACCATATTTTGTATCGGACGGGTCTCCGGTCAACACCTTTGAGTTCATCCGACCACTGCTCAGAAGCTTGGATTATGATCTACCAAAGGCTTCATTGTCTGTACCTCAAGCTCTTCGTTTAGGAAGGATTTTTTGGGCAGTCTATACAATATTATATCCATGGTTGGACCGATGGTGGATTCCTCAGCCGCTTATCCTTCCTGCTGAAGTATACAAGGTTGGTGTTACACACTACTGCTCTTATCTTAAAGCCAAAGAAGAGCTTGGCTATGCCCCAATGGTGACTCCTCGGGAGGGCATGGCTACTACAATCTCATACTGGCAGGAGAGGAAAAGGAAAACTCTGGACGGGCCTACAATATACGCATGGCTATTTTGTGTGATTGGCATGGGTACAGTATTTTGTGCTGCTTATTTGCCAGACGTAGGACCTGTACCACTCTTTAGAGCTGTTAGCCTCTTCTTTTTCCGGTCAATGTGGATAGTGCGGATGGTATTCCTACTAGCCACTGCATTGCATGTTGGAGAGGCTGTGTACGCGTGGAAACTGGCAAAACAGGTCGATCCTGCTAACGCGAGAGGATGGTTCTGGCAGACCTTTGCTCTCGGGGTGTTTTCACTACGTCTTTTGTTGAAGAAAGCAAAGAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

481

Amino Acids

53.96

Weight (kDa)

9.12

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 12 - 127 1.6e-06 NmrA-like family
3Beta_HSD PF01073 15 - 295 2.8e-83 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 15 - 242 1.5e-33 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 15 - 130 4.5e-09 Polysaccharide biosynthesis protein
RmlD_sub_bind PF04321 15 - 175 2.1e-09 RmlD substrate binding domain
GDP_Man_Dehyd PF16363 16 - 241 1.1e-18 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 16 - 236 6e-07 Male sterility protein
NAD_binding_10 PF13460 18 - 132 9.2e-12 NAD(P)H-binding
TMEM254 PF14934 389 - 475 1.6e-29 Transmembrane protein 254
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33630
fragaria_vesca FvH4_5g26130 FvH4_5g26130
malus_domestica MD06G1004300.v1.1
prunus_persica Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1
pyrus_communis pycom06g00410
rosa_chinensis RchiOBHm_Chr7g0216661
rosa_laevigata RLG00000002552
rosa_multiflora Rmu_sc0029435.1_g000001 Rmu_ssc0000117.1_g000009
rosa_roxburghii Rroxscaffold_3G00243030
rosa_rugosa Rorug07G0161500
rosa_samantha Rh7AG301300 Rh7BG292800 Rh7CG319900 Rh7DG301900
rosa_wichuraiana Rw7G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 606
AccI GTMKAC 2 cut(s) 557, 1002
AccII CGCG 2 cut(s) 1331, 1367
AciI CCGC 7 cut(s) 54, 75, 130, 229, 566, 980, 1279
AclWI GGATC 3 cut(s) 461, 474, 1349
AcoI YGGCCR 1 cut(s) 787
AcuI CTGAAG 1 cut(s) 1017
AfaI GTAC 6 cut(s) 263, 515, 894, 1186, 1227, 1328
AfiI CCNNNNNNNGG 3 cut(s) 147, 1085, 1217
AflII CTTAAG 1 cut(s) 509
AflIII ACRYGT 1 cut(s) 1329
AgsI TTSAA 2 cut(s) 655, 1426
AjnI CCWGG 3 cut(s) 84, 592, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 907, 939
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 9 cut(s) 91, 107, 441, 578, 782, 858, 902, 1054, 1241
AluI AGCT 9 cut(s) 91, 107, 441, 578, 782, 858, 902, 1054, 1241
Alw21I GWGCWC 3 cut(s) 109, 386, 711
Alw26I GTCTC 2 cut(s) 353, 819
AlwI GGATC 3 cut(s) 461, 474, 1349
Ama87I CYCGRG 2 cut(s) 1080, 1397
AoxI GGCC 3 cut(s) 432, 787, 1142
ApeKI GCWGC 5 cut(s) 33, 286, 578, 782, 1199
AspS9I GGNCC 6 cut(s) 150, 433, 590, 958, 1142, 1220
AsuHPI GGTGA 3 cut(s) 40, 608, 1084
AvaI CYCGRG 2 cut(s) 1080, 1397
AvaII GGWCC 4 cut(s) 150, 590, 958, 1220
BalI TGGCCA 1 cut(s) 789
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 606
BanII GRGCYC 2 cut(s) 74, 109
Bbv12I GWGCWC 3 cut(s) 109, 386, 711
BbvCI CCTCAGC 1 cut(s) 975
BbvI GCAGC 5 cut(s) 45, 273, 565, 769, 1186
BccI CCATC 4 cut(s) 165, 957, 1276, 1368
BceAI ACGGC 2 cut(s) 46, 490
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 3 cut(s) 86, 594, 753
BciVI GTATCC 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 353, 819
BfaI CTAG 4 cut(s) 254, 629, 704, 1295
BfrI CTTAAG 1 cut(s) 509
BfuI GTATCC 1 cut(s) 334
BisI GCNGC 6 cut(s) 34, 287, 579, 783, 980, 1200
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 6 cut(s) 35, 288, 580, 784, 981, 1201
Bme1390I CCNGG 3 cut(s) 86, 594, 753
Bme18I GGWCC 4 cut(s) 150, 590, 958, 1220
BmeT110I CYCGRG 2 cut(s) 1080, 1397
BmgT120I GGNCC 6 cut(s) 150, 433, 590, 958, 1142, 1220
BmiI GGNNCC 4 cut(s) 73, 152, 468, 608
BmrFI CCNGG 3 cut(s) 86, 594, 753
BmsI GCATC 2 cut(s) 33, 192
BpmI CTGGAG 1 cut(s) 107
Bpu10I CCTNAGC 3 cut(s) 8, 437, 975
Bpu1102I GCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 882
BsaBI GATNNNNATC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 819
BsaJI CCNNGG 7 cut(s) 146, 169, 187, 614, 752, 950, 1079
BsaWI WCCGGW 2 cut(s) 817, 1260
BsaXI ACNNNNNCTCC 2 cut(s) 1308, 1338
Bsc4I CCNNNNNNNGG 3 cut(s) 147, 1085, 1217
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 3 cut(s) 689, 1115, 1344
Bse3DI GCAATG 2 cut(s) 489, 1304
Bse8I GATNNNNATC 1 cut(s) 867
BseBI CCWGG 3 cut(s) 86, 594, 753
BseDI CCNNGG 7 cut(s) 146, 169, 187, 614, 752, 950, 1079
BseGI GGATG 6 cut(s) 183, 199, 217, 837, 1287, 1379
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 3 cut(s) 147, 1085, 1217
BseMI GCAATG 2 cut(s) 489, 1304
BseMII CTCAG 3 cut(s) 486, 865, 989
BseNI ACTGG 3 cut(s) 689, 1115, 1344
BseRI GAGGAG 4 cut(s) 117, 130, 152, 1068
BseXI GCAGC 5 cut(s) 45, 273, 565, 769, 1186
Bsh1236I CGCG 2 cut(s) 1331, 1367
BshFI GGCC 3 cut(s) 434, 789, 1144
BshNI GGYRCC 1 cut(s) 606
BsiHKAI GWGCWC 3 cut(s) 109, 386, 711
BsiHKCI CYCGRG 2 cut(s) 1080, 1397
BsiSI CCGG 3 cut(s) 51, 818, 1261
BsiWI CGTACG 1 cut(s) 261
BslFI GGGAC 3 cut(s) 136, 610, 747
BslI CCNNNNNNNGG 3 cut(s) 147, 1085, 1217
BsmAI GTCTC 2 cut(s) 353, 819
BsmFI GGGAC 3 cut(s) 136, 610, 747
BsnI GGCC 3 cut(s) 434, 789, 1144
Bso31I GGTCTC 1 cut(s) 819
BsoBI CYCGRG 2 cut(s) 1080, 1397
Bsp1286I GDGCHC 4 cut(s) 74, 109, 386, 711
Bsp143I GATC 3 cut(s) 466, 868, 1354
Bsp1720I GCTNAGC 1 cut(s) 495
Bsp19I CCATGG 1 cut(s) 950
BspACI CCGC 7 cut(s) 54, 75, 130, 229, 566, 980, 1279
BspANI GGCC 3 cut(s) 434, 789, 1144
BspCNI CTCAG 3 cut(s) 487, 864, 988
BspFNI CGCG 2 cut(s) 1331, 1367
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 4 cut(s) 73, 152, 468, 608
BspPI GGATC 3 cut(s) 461, 474, 1349
BspQI GCTCTTC 2 cut(s) 909, 1044
BspT107I GGYRCC 1 cut(s) 606
BspTI CTTAAG 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 819
BsrDI GCAATG 2 cut(s) 489, 1304
BsrFI RCCGGY 1 cut(s) 50
BsrI ACTGG 3 cut(s) 689, 1115, 1344
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 7 cut(s) 146, 169, 187, 614, 752, 950, 1079
BssMI GATC 3 cut(s) 466, 868, 1354
BssNAI GTATAC 2 cut(s) 558, 1003
BssT1I CCWWGG 4 cut(s) 146, 169, 187, 950
Bst1107I GTATAC 2 cut(s) 558, 1003
Bst2UI CCWGG 3 cut(s) 86, 594, 753
Bst4CI ACNGT 1 cut(s) 1189
Bst6I CTCTTC 3 cut(s) 909, 1044, 1256
BstAFI CTTAAG 1 cut(s) 509
BstC8I GCNNGC 3 cut(s) 711, 727, 787
BstDEI CTNAG 5 cut(s) 8, 437, 495, 851, 975
BstDSI CCRYGG 1 cut(s) 950
BstEII GGTNACC 1 cut(s) 46
BstF5I GGATG 6 cut(s) 183, 199, 217, 837, 1287, 1379
BstFNI CGCG 2 cut(s) 1331, 1367
BstKTI GATC 3 cut(s) 469, 871, 1357
BstMAI GTCTC 2 cut(s) 353, 819
BstMBI GATC 3 cut(s) 466, 868, 1354
BstMWI GCNNNNNNNGC 6 cut(s) 30, 60, 69, 295, 1328, 1439
BstNI CCWGG 3 cut(s) 86, 594, 753
BstNSI RCATGY 1 cut(s) 1313
BstPI GGTNACC 1 cut(s) 46
BstSCI CCNGG 3 cut(s) 84, 592, 751
BstUI CGCG 2 cut(s) 1331, 1367
BstV1I GCAGC 5 cut(s) 45, 273, 565, 769, 1186
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BstZ17I GTATAC 2 cut(s) 558, 1003
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 3 cut(s) 434, 789, 1144
BtgI CCRYGG 1 cut(s) 950
BtsCI GGATG 6 cut(s) 183, 199, 217, 837, 1287, 1379
BtsI GCAGTG 2 cut(s) 845, 1299
BtsIMutI CAGTG 2 cut(s) 845, 1299
Cac8I GCNNGC 3 cut(s) 711, 727, 787
CciI TCATGA 1 cut(s) 460
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 6 cut(s) 150, 433, 590, 958, 1142, 1220
CsiI ACCWGGT 1 cut(s) 592
Csp6I GTAC 6 cut(s) 262, 514, 893, 1185, 1226, 1327
CviAII CATG 9 cut(s) 461, 730, 743, 951, 1090, 1158, 1180, 1310, 1443
CviQI GTAC 6 cut(s) 262, 514, 893, 1185, 1226, 1327
DdeI CTNAG 5 cut(s) 8, 437, 495, 851, 975
DpnI GATC 3 cut(s) 468, 870, 1356
DpnII GATC 3 cut(s) 466, 868, 1354
EaeI YGGCCR 1 cut(s) 787
Eam1104I CTCTTC 3 cut(s) 909, 1044, 1256
EarI CTCTTC 3 cut(s) 909, 1044, 1256
Ecl136II GAGCTC 1 cut(s) 107
Eco130I CCWWGG 4 cut(s) 146, 169, 187, 950
Eco24I GRGCYC 2 cut(s) 74, 109
Eco31I GGTCTC 1 cut(s) 819
Eco47I GGWCC 4 cut(s) 150, 590, 958, 1220
Eco53kI GAGCTC 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 1017
Eco88I CYCGRG 2 cut(s) 1080, 1397
Eco91I GGTNACC 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 107
EcoO109I RGGNCCY 2 cut(s) 433, 1220
EcoO65I GGTNACC 1 cut(s) 46
EcoRII CCWGG 3 cut(s) 84, 592, 751
EcoT14I CCWWGG 4 cut(s) 146, 169, 187, 950
EcoT38I GRGCYC 2 cut(s) 74, 109
ErhI CCWWGG 4 cut(s) 146, 169, 187, 950
FaeI CATG 9 cut(s) 464, 733, 746, 954, 1093, 1161, 1183, 1313, 1446
FalI AAGNNNNNCTT 4 cut(s) 626, 658, 1038, 1070
FaqI GGGAC 3 cut(s) 136, 610, 747
FatI CATG 9 cut(s) 460, 729, 742, 950, 1089, 1157, 1179, 1309, 1442
FauI CCCGC 1 cut(s) 222
FblI GTMKAC 2 cut(s) 557, 1002
Fnu4HI GCNGC 6 cut(s) 34, 287, 579, 783, 980, 1200
FokI GGATG 6 cut(s) 170, 206, 224, 824, 1294, 1386
FriOI GRGCYC 2 cut(s) 74, 109
Fsp4HI GCNGC 6 cut(s) 34, 287, 579, 783, 980, 1200
FspBI CTAG 4 cut(s) 254, 629, 704, 1295
GluI GCNGC 6 cut(s) 34, 287, 579, 783, 980, 1200
GsuI CTGGAG 1 cut(s) 107
HaeIII GGCC 3 cut(s) 434, 789, 1144
HapII CCGG 3 cut(s) 51, 818, 1261
Hin1II CATG 9 cut(s) 464, 733, 746, 954, 1093, 1161, 1183, 1313, 1446
HincII GTYRAC 3 cut(s) 178, 202, 823
HindII GTYRAC 3 cut(s) 178, 202, 823
HindIII AAGCTT 1 cut(s) 856
HinfI GANTC 2 cut(s) 970, 1075
HpaI GTTAAC 1 cut(s) 202
HpaII CCGG 3 cut(s) 51, 818, 1261
HphI GGTGA 3 cut(s) 40, 608, 1084
Hpy166II GTNNAC 6 cut(s) 178, 202, 558, 823, 1003, 1327
Hpy188I TCNGA 6 cut(s) 98, 119, 615, 808, 842, 854
Hpy188III TCNNGA 6 cut(s) 273, 350, 461, 532, 1082, 1136
Hpy8I GTNNAC 6 cut(s) 178, 202, 558, 823, 1003, 1327
Hpy99I CGWCG 2 cut(s) 32, 102
HpyAV CCTTC 6 cut(s) 14, 214, 620, 909, 998, 1432
HpyCH4III ACNGT 1 cut(s) 1189
HpyCH4IV ACGT 2 cut(s) 1215, 1414
HpyCH4V TGCA 6 cut(s) 4, 183, 289, 742, 1304, 1309
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 60, 69, 295, 1328, 1439
HpyF3I CTNAG 5 cut(s) 8, 437, 495, 851, 975
HpySE526I ACGT 2 cut(s) 1215, 1414
Hsp92II CATG 9 cut(s) 464, 733, 746, 954, 1093, 1161, 1183, 1313, 1446
KspAI GTTAAC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 466, 868, 1354
LguI GCTCTTC 2 cut(s) 909, 1044
LmnI GCTCC 3 cut(s) 77, 88, 104
Lsp1109I GCAGC 5 cut(s) 45, 273, 565, 769, 1186
LweI GCATC 2 cut(s) 33, 192
MabI ACCWGGT 1 cut(s) 592
MaeI CTAG 4 cut(s) 254, 629, 704, 1295
MaeII ACGT 2 cut(s) 1215, 1414
MaeIII GTNAC 3 cut(s) 46, 1015, 1072
MalI GATC 3 cut(s) 468, 870, 1356
MboI GATC 3 cut(s) 466, 868, 1354
MboII GAAGA 6 cut(s) 550, 611, 896, 1061, 1243, 1438
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 4 cut(s) 74, 109, 386, 711
MlsI TGGCCA 1 cut(s) 789
MluCI AATT 3 cut(s) 290, 645, 657
MluI ACGCGT 1 cut(s) 1329
MluNI TGGCCA 1 cut(s) 789
MlyI GAGTC 1 cut(s) 1069
MmeI TCCRAC 4 cut(s) 121, 865, 936, 1294
Mox20I TGGCCA 1 cut(s) 789
MscI TGGCCA 1 cut(s) 789
MseI TTAA 5 cut(s) 201, 312, 510, 644, 1038
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 789
MspA1I CMGCKG 1 cut(s) 578
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 3 cut(s) 51, 818, 1261
MspR9I CCNGG 3 cut(s) 86, 594, 753
MvaI CCWGG 3 cut(s) 86, 594, 753
MvnI CGCG 2 cut(s) 1331, 1367
MwoI GCNNNNNNNGC 6 cut(s) 30, 60, 69, 295, 1328, 1439
NcoI CCATGG 1 cut(s) 950
NdeII GATC 3 cut(s) 466, 868, 1354
NlaIII CATG 9 cut(s) 464, 733, 746, 954, 1093, 1161, 1183, 1313, 1446
NlaIV GGNNCC 4 cut(s) 73, 152, 468, 608
NmuCI GTSAC 2 cut(s) 46, 1072
NspI RCATGY 1 cut(s) 1313
OliI CACNNNNGTG 1 cut(s) 41
PagI TCATGA 1 cut(s) 460
PciSI GCTCTTC 2 cut(s) 909, 1044
PcsI WCGNNNNNNNCGW 2 cut(s) 106, 115
PfeI GAWTC 1 cut(s) 970
Pfl23II CGTACG 1 cut(s) 261
PkrI GCNGC 6 cut(s) 35, 288, 580, 784, 981, 1201
PleI GAGTC 1 cut(s) 1069
PpsI GAGTC 1 cut(s) 1069
PpuMI RGGWCCY 1 cut(s) 1220
Psp124BI GAGCTC 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 1220
Psp6I CCWGG 3 cut(s) 84, 592, 751
PspEI GGTNACC 1 cut(s) 46
PspGI CCWGG 3 cut(s) 84, 592, 751
PspLI CGTACG 1 cut(s) 261
PspN4I GGNNCC 4 cut(s) 73, 152, 468, 608
PspPI GGNCC 6 cut(s) 150, 433, 590, 958, 1142, 1220
PspPPI RGGWCCY 1 cut(s) 1220
PsuI RGATCY 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 6 cut(s) 263, 515, 894, 1186, 1227, 1328
RsaNI GTAC 6 cut(s) 262, 514, 893, 1185, 1226, 1327
RseI CAYNNNNRTG 1 cut(s) 41
SacI GAGCTC 1 cut(s) 109
SapI GCTCTTC 2 cut(s) 909, 1044
SaqAI TTAA 5 cut(s) 201, 312, 510, 644, 1038
SatI GCNGC 6 cut(s) 34, 287, 579, 783, 980, 1200
Sau3AI GATC 3 cut(s) 466, 868, 1354
Sau96I GGNCC 6 cut(s) 150, 433, 590, 958, 1142, 1220
SchI GAGTC 1 cut(s) 1069
ScrFI CCNGG 3 cut(s) 86, 594, 753
SduI GDGCHC 4 cut(s) 74, 109, 386, 711
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 2 cut(s) 33, 192
SgrAI CRCCGGYG 1 cut(s) 50
SinI GGWCC 4 cut(s) 150, 590, 958, 1220
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 2 cut(s) 509, 897
SmoI CTYRAG 2 cut(s) 509, 897
Sse9I AATT 3 cut(s) 290, 645, 657
SsiI CCGC 7 cut(s) 54, 75, 130, 229, 566, 980, 1279
SspI AATATT 1 cut(s) 942
SspMI CTAG 4 cut(s) 254, 629, 704, 1295
SstI GAGCTC 1 cut(s) 109
StyD4I CCNGG 3 cut(s) 84, 592, 751
StyI CCWWGG 4 cut(s) 146, 169, 187, 950
TaaI ACNGT 1 cut(s) 1189
TaiI ACGT 2 cut(s) 1218, 1417
TaqI TCGA 4 cut(s) 27, 351, 363, 1353
TaqII GACCGA 1 cut(s) 975
TasI AATT 3 cut(s) 290, 645, 657
TatI WGTACW 1 cut(s) 513
TauI GCSGC 1 cut(s) 982
TfiI GAWTC 1 cut(s) 970
Tru1I TTAA 5 cut(s) 201, 312, 510, 644, 1038
Tru9I TTAA 5 cut(s) 201, 312, 510, 644, 1038
TscAI CASTG 2 cut(s) 852, 1306
TseFI GTSAC 2 cut(s) 46, 1072
TseI GCWGC 5 cut(s) 33, 286, 578, 782, 1199
Tsp45I GTSAC 2 cut(s) 46, 1072
TspDTI ATGAA 3 cut(s) 33, 826, 873
TspGWI ACGGA 2 cut(s) 279, 639
TspRI CASTG 2 cut(s) 852, 1306
Vha464I CTTAAG 1 cut(s) 509
VpaK11BI GGWCC 4 cut(s) 150, 590, 958, 1220
XceI RCATGY 1 cut(s) 1313
XmiI GTMKAC 2 cut(s) 557, 1002
XspI CTAG 4 cut(s) 254, 629, 704, 1295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.