FvH4_5g26130

Short-chain dehydrogenase reductase family 42E member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0012481
Physical Location & Seq
Reverse (-)
17578389 .. 17581273
2885 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26130.t1

Sequence Viewer

Length: 1584 bp
ATGACGGAGCTTCACACGTACTCCACTCCAGACAGCCAAAGTACTTTCGCTTGGATCGCATCTCCTTATAATCACACTGGCACTTGTTCTGACTCGTGCAAGTCTGATTCAAGTAAACCCATCATCCCAAAACAAATCATGCACCTCACCGAAAACGAAGGCATCGACGGCACCACTTTCGTCGTCACCGGCGGCCTGGGCTTCGTCGGCTCCGCACTCTGCTTGGAGCTCGTCCGACGTGGAGCCCGTCAAGTCCGAGCCTTCGACCGCAGAACCTCCTCCCCTTGGTCCCACCTCCTCTTCGACCAAGGCGTCAACTGCATCCAAGGGGATGTTGTTAACAAAAAGGATGTGGAGAAGGCACTACGAGGGGCGGGTTGTGTGTTCCACCTCGCCTCGTACGGAATGTCGGGGAAGGAAATGCTGCAGTTTGGGCGTGTTGACGAGGTTAATATCAATGGAACTTGTCATATATTGGACACTTGTCTCGATTTCGGGGTCGAAAGGCTTGTCTATGTAAGCACATACAATGTTGTGTTTGGGGGAAAGGAGATTGTGAGTGGGAATGAGGCCCTGAGCTATTTTCCTATTGATAGTCATGAGGATCCGTACGGCAGAAGTAAATCCATTGCTGAGCAGTTGGTTCTTAAGTACAATGCTCGTCCCTTCAGGCAAAACAATAATGGGAAGTGTCTTTATACCTGTGCGATTCGTCCTGCTGCTATCTATGGACCTGGTGAAGAAAGGCACCTTCCGAGGATAGTGTCCCTTGCAAAGTTGGGCTTAATTCCGTTCAAAATTGGTGAAGCAAATGTGAAAACAGACTGGATTTATGTGGATAACTTAGTACTTGCACTAGTGTTGGCGAGCATGGGACTGTTGCATGACATTCCTGGGAGAGAGAAAGAAAAACAACCCATAGCTGCTGGCCAACCTTATTTTGTATCAGACGGGTCTCCGGTCAACACCTTTGAGTTCATCCGACCACTGCTCAGAAGCTTGGATTATGATCTACCAAAGTCTTCATTATCTGTACCTCAAGCTCTTCGTTTAGGAAGGATTTTTTGGGGAGTTTATACAGTATTATATCCATGGTTGGACCGATGGTGGATTCCTCAGCCGCTTATCCTTCCTGCTGAAGTATACAAGGTTGGTGTTACCCACTACTTCTCTTACCTTAAAGCCAAGGAAGAGCTTGGCTATGTTCCAATGGTGACTCCTCGGGAGGGCATGGCTACTACAATCTCATACTGGCAGGAGAGGAAAAGGAAAACTCTGGATGGGCCTACAATATATGCATGGCTATTTTGTGTGATTGGCATGGGTATGGTATTTTGTGCCGCTTATTTGCCAGACATCGGACCTGTACCGCTCTTTAGAGCTATTAGCCTCTTCTTTTTCCGGTCAATGCGGATAGTGAGGATAGTATTCCTTCTAGCTACTGCATTGCATGTTGGAGAGGCTGTATATGCTTGGAGCCTGGCAAAAAAGGTAGATCCTGCTAACACAAGAGGATGGTTCTGGCAGACCTTTGCTCTCGGGGTGTTTTCACTACGTCTTTTGTTGAAGAGAGCAAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

528

Amino Acids

59.13

Weight (kDa)

9.0

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 60 - 221 8.8e-09 RmlD substrate binding domain
3Beta_HSD PF01073 61 - 342 4.2e-82 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 61 - 289 3.7e-32 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 61 - 176 7.3e-08 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 62 - 288 1.3e-17 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 62 - 280 4.3e-06 Male sterility protein
NAD_binding_10 PF13460 64 - 178 2.6e-10 NAD(P)H-binding
TMEM254 PF14934 436 - 522 1.6e-29 Transmembrane protein 254
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33630
fragaria_vesca FvH4_5g26130 FvH4_5g26130
malus_domestica MD06G1004300.v1.1
prunus_persica Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1
pyrus_communis pycom06g00410
rosa_chinensis RchiOBHm_Chr7g0216661
rosa_laevigata RLG00000002552
rosa_multiflora Rmu_sc0029435.1_g000001 Rmu_ssc0000117.1_g000009
rosa_roxburghii Rroxscaffold_3G00243030
rosa_rugosa Rorug07G0161500
rosa_samantha Rh7AG301300 Rh7BG292800 Rh7CG319900 Rh7DG301900
rosa_wichuraiana Rw7G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 69
AasI GACNNNNNNGTC 1 cut(s) 311
AccB1I GGYRCC 2 cut(s) 170, 747
AccBSI CCGCTC 1 cut(s) 1372
AccI GTMKAC 1 cut(s) 1143
AciI CCGC 8 cut(s) 192, 213, 268, 374, 1121, 1341, 1370, 1411
AclWI GGATC 4 cut(s) 62, 599, 612, 1490
AcoI YGGCCR 1 cut(s) 928
AcuI CTGAAG 2 cut(s) 652, 1158
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 8 cut(s) 20, 43, 401, 611, 653, 849, 1035, 1368
AfiI CCNNNNNNNGG 3 cut(s) 285, 1226, 1358
AflII CTTAAG 1 cut(s) 647
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 3 cut(s) 111, 796, 1567
AhlI ACTAGT 1 cut(s) 856
AjiI CACGTC 1 cut(s) 239
AjnI CCWGG 4 cut(s) 195, 733, 892, 1479
AjuI GAANNNNNNNTTGG 2 cut(s) 1048, 1080
AluBI AGCT 9 cut(s) 10, 229, 579, 923, 999, 1043, 1195, 1382, 1439
AluI AGCT 9 cut(s) 10, 229, 579, 923, 999, 1043, 1195, 1382, 1439
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 2 cut(s) 491, 960
AlwI GGATC 4 cut(s) 62, 599, 612, 1490
Ama87I CYCGRG 2 cut(s) 1221, 1538
AoxI GGCC 4 cut(s) 193, 570, 928, 1283
ApeKI GCWGC 3 cut(s) 424, 719, 923
ArsI GACNNNNNNTTYG 2 cut(s) 495, 527
AspS9I GGNCC 6 cut(s) 288, 571, 731, 1099, 1283, 1361
AsuHPI GGTGA 5 cut(s) 139, 178, 749, 815, 1225
AvaI CYCGRG 2 cut(s) 1221, 1538
AvaII GGWCC 4 cut(s) 288, 731, 1099, 1361
BalI TGGCCA 1 cut(s) 930
BamHI GGATCC 1 cut(s) 604
BanI GGYRCC 2 cut(s) 170, 747
BanII GRGCYC 2 cut(s) 231, 247
BauI CACGAG 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 1014
Bbv12I GWGCWC 1 cut(s) 231
BbvCI CCTCAGC 1 cut(s) 1116
BbvI GCAGC 3 cut(s) 411, 706, 910
BccI CCATC 4 cut(s) 128, 1098, 1274, 1509
BceAI ACGGC 2 cut(s) 184, 628
BcgI CGANNNNNNTGC 2 cut(s) 160, 194
BciT130I CCWGG 4 cut(s) 197, 735, 894, 1481
BcoDI GTCTC 2 cut(s) 491, 960
BcuI ACTAGT 1 cut(s) 856
BfaI CTAG 2 cut(s) 857, 1436
BfmI CTRYAG 1 cut(s) 425
BfrI CTTAAG 1 cut(s) 647
BisI GCNGC 6 cut(s) 193, 425, 720, 924, 1121, 1341
BlpI GCTNAGC 1 cut(s) 633
BlsI GCNGC 6 cut(s) 194, 426, 721, 925, 1122, 1342
BmcAI AGTACT 2 cut(s) 43, 849
Bme1390I CCNGG 4 cut(s) 197, 735, 894, 1481
Bme18I GGWCC 4 cut(s) 288, 731, 1099, 1361
BmeT110I CYCGRG 2 cut(s) 1221, 1538
BmgBI CACGTC 1 cut(s) 239
BmgT120I GGNCC 6 cut(s) 288, 571, 731, 1099, 1283, 1361
BmiI GGNNCC 7 cut(s) 172, 211, 244, 290, 606, 749, 1478
BmrFI CCNGG 4 cut(s) 197, 735, 894, 1481
BmsI GCATC 3 cut(s) 68, 171, 330
BoxI GACNNNNGTC 1 cut(s) 483
BpiI GAAGAC 1 cut(s) 1014
BpmI CTGGAG 1 cut(s) 12
Bpu10I CCTNAGC 2 cut(s) 575, 1116
Bpu1102I GCTNAGC 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 1023
BsaAI YACGTR 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 1008
BsaHI GRCGYC 1 cut(s) 312
BsaI GGTCTC 1 cut(s) 960
BsaJI CCNNGG 9 cut(s) 196, 284, 307, 325, 755, 893, 1091, 1185, 1220
BsaWI WCCGGW 2 cut(s) 958, 1401
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 1449, 1479
Bsc4I CCNNNNNNNGG 3 cut(s) 285, 1226, 1358
Bse118I RCCGGY 1 cut(s) 188
Bse1I ACTGG 3 cut(s) 82, 830, 1256
Bse3DI GCAATG 2 cut(s) 627, 1445
Bse8I GATNNNNATC 1 cut(s) 1008
BseBI CCWGG 4 cut(s) 197, 735, 894, 1481
BseDI CCNNGG 9 cut(s) 196, 284, 307, 325, 755, 893, 1091, 1185, 1220
BseGI GGATG 7 cut(s) 123, 321, 337, 355, 978, 1285, 1520
BseJI GATNNNNATC 1 cut(s) 1008
BseLI CCNNNNNNNGG 3 cut(s) 285, 1226, 1358
BseMI GCAATG 2 cut(s) 627, 1445
BseMII CTCAG 4 cut(s) 566, 624, 1006, 1130
BseNI ACTGG 3 cut(s) 82, 830, 1256
BseRI GAGGAG 3 cut(s) 268, 287, 1209
BseXI GCAGC 3 cut(s) 411, 706, 910
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 4 cut(s) 195, 572, 930, 1285
BshNI GGYRCC 2 cut(s) 170, 747
BsiEI CGRYCG 1 cut(s) 268
BsiHKAI GWGCWC 1 cut(s) 231
BsiHKCI CYCGRG 2 cut(s) 1221, 1538
BsiSI CCGG 3 cut(s) 189, 959, 1402
BsiWI CGTACG 2 cut(s) 399, 609
BslFI GGGAC 4 cut(s) 274, 648, 751, 888
BslI CCNNNNNNNGG 3 cut(s) 285, 1226, 1358
BsmAI GTCTC 2 cut(s) 491, 960
BsmFI GGGAC 4 cut(s) 274, 648, 751, 888
BsnI GGCC 4 cut(s) 195, 572, 930, 1285
Bso31I GGTCTC 1 cut(s) 960
BsoBI CYCGRG 2 cut(s) 1221, 1538
Bsp1286I GDGCHC 2 cut(s) 231, 247
Bsp143I GATC 4 cut(s) 54, 604, 1009, 1495
Bsp1720I GCTNAGC 1 cut(s) 633
Bsp19I CCATGG 1 cut(s) 1091
BspACI CCGC 8 cut(s) 192, 213, 268, 374, 1121, 1341, 1370, 1411
BspANI GGCC 4 cut(s) 195, 572, 930, 1285
BspCNI CTCAG 4 cut(s) 567, 625, 1005, 1129
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 7 cut(s) 172, 211, 244, 290, 606, 749, 1478
BspMAI CTGCAG 1 cut(s) 429
BspPI GGATC 4 cut(s) 62, 599, 612, 1490
BspQI GCTCTTC 2 cut(s) 1050, 1185
BspT107I GGYRCC 2 cut(s) 170, 747
BspTI CTTAAG 1 cut(s) 647
BspTNI GGTCTC 1 cut(s) 960
BsrBI CCGCTC 1 cut(s) 1372
BsrDI GCAATG 2 cut(s) 627, 1445
BsrFI RCCGGY 1 cut(s) 188
BsrI ACTGG 3 cut(s) 82, 830, 1256
BssAI RCCGGY 1 cut(s) 188
BssECI CCNNGG 9 cut(s) 196, 284, 307, 325, 755, 893, 1091, 1185, 1220
BssMI GATC 4 cut(s) 54, 604, 1009, 1495
BssNAI GTATAC 1 cut(s) 1144
BssNI GRCGYC 1 cut(s) 312
BssSI CACGAG 1 cut(s) 94
BssT1I CCWWGG 5 cut(s) 284, 307, 325, 1091, 1185
Bst1107I GTATAC 1 cut(s) 1144
Bst2BI CACGAG 1 cut(s) 94
Bst2UI CCWGG 4 cut(s) 197, 735, 894, 1481
Bst4CI ACNGT 2 cut(s) 879, 1081
Bst6I CTCTTC 5 cut(s) 305, 1050, 1185, 1397, 1562
BstACI GRCGYC 1 cut(s) 312
BstAFI CTTAAG 1 cut(s) 647
BstBAI YACGTR 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 868, 928
BstDEI CTNAG 5 cut(s) 575, 633, 844, 992, 1116
BstDSI CCRYGG 1 cut(s) 1091
BstF5I GGATG 7 cut(s) 123, 321, 337, 355, 978, 1285, 1520
BstKTI GATC 4 cut(s) 57, 607, 1012, 1498
BstMAI GTCTC 2 cut(s) 491, 960
BstMBI GATC 4 cut(s) 54, 604, 1009, 1495
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 7 cut(s) 56, 168, 198, 207, 318, 433, 1469
BstNI CCWGG 4 cut(s) 197, 735, 894, 1481
BstNSI RCATGY 1 cut(s) 1454
BstPAI GACNNNNGTC 1 cut(s) 483
BstSCI CCNGG 4 cut(s) 195, 733, 892, 1479
BstSFI CTRYAG 1 cut(s) 425
BstV1I GCAGC 3 cut(s) 411, 706, 910
BstV2I GAAGAC 1 cut(s) 1014
BstX2I RGATCY 2 cut(s) 604, 1495
BstYI RGATCY 2 cut(s) 604, 1495
BstZ17I GTATAC 1 cut(s) 1144
BsuRI GGCC 4 cut(s) 195, 572, 930, 1285
BtgI CCRYGG 1 cut(s) 1091
BtrI CACGTC 1 cut(s) 239
BtsCI GGATG 7 cut(s) 123, 321, 337, 355, 978, 1285, 1520
BtsI GCAGTG 1 cut(s) 986
BtsIMutI CAGTG 2 cut(s) 75, 986
Cac8I GCNNGC 2 cut(s) 868, 928
CciI TCATGA 1 cut(s) 598
Cfr10I RCCGGY 1 cut(s) 188
Cfr13I GGNCC 6 cut(s) 288, 571, 731, 1099, 1283, 1361
CseI GACGC 1 cut(s) 301
CsiI ACCWGGT 1 cut(s) 733
Csp6I GTAC 8 cut(s) 19, 42, 400, 610, 652, 848, 1034, 1367
CviAII CATG 9 cut(s) 139, 599, 871, 884, 1092, 1231, 1299, 1321, 1451
CviQI GTAC 8 cut(s) 19, 42, 400, 610, 652, 848, 1034, 1367
DdeI CTNAG 5 cut(s) 575, 633, 844, 992, 1116
DpnI GATC 4 cut(s) 56, 606, 1011, 1497
DpnII GATC 4 cut(s) 54, 604, 1009, 1495
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 1 cut(s) 928
Eam1104I CTCTTC 5 cut(s) 305, 1050, 1185, 1397, 1562
EarI CTCTTC 5 cut(s) 305, 1050, 1185, 1397, 1562
Ecl136II GAGCTC 1 cut(s) 229
Eco130I CCWWGG 5 cut(s) 284, 307, 325, 1091, 1185
Eco24I GRGCYC 2 cut(s) 231, 247
Eco31I GGTCTC 1 cut(s) 960
Eco47I GGWCC 4 cut(s) 288, 731, 1099, 1361
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 2 cut(s) 652, 1158
Eco88I CYCGRG 2 cut(s) 1221, 1538
EcoICRI GAGCTC 1 cut(s) 229
EcoO109I RGGNCCY 1 cut(s) 571
EcoRII CCWGG 4 cut(s) 195, 733, 892, 1479
EcoT14I CCWWGG 5 cut(s) 284, 307, 325, 1091, 1185
EcoT22I ATGCAT 1 cut(s) 1300
EcoT38I GRGCYC 2 cut(s) 231, 247
ErhI CCWWGG 5 cut(s) 284, 307, 325, 1091, 1185
FaeI CATG 9 cut(s) 142, 602, 874, 887, 1095, 1234, 1302, 1324, 1454
FalI AAGNNNNNCTT 2 cut(s) 767, 799
FaqI GGGAC 4 cut(s) 274, 648, 751, 888
FatI CATG 9 cut(s) 138, 598, 870, 883, 1091, 1230, 1298, 1320, 1450
FauI CCCGC 1 cut(s) 367
FblI GTMKAC 1 cut(s) 1143
Fnu4HI GCNGC 6 cut(s) 193, 425, 720, 924, 1121, 1341
FokI GGATG 7 cut(s) 110, 308, 344, 362, 965, 1292, 1527
FriOI GRGCYC 2 cut(s) 231, 247
Fsp4HI GCNGC 6 cut(s) 193, 425, 720, 924, 1121, 1341
FspBI CTAG 2 cut(s) 857, 1436
GluI GCNGC 6 cut(s) 193, 425, 720, 924, 1121, 1341
GsuI CTGGAG 1 cut(s) 12
HaeIII GGCC 4 cut(s) 195, 572, 930, 1285
HapII CCGG 3 cut(s) 189, 959, 1402
HgaI GACGC 1 cut(s) 301
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 9 cut(s) 142, 602, 874, 887, 1095, 1234, 1302, 1324, 1454
HincII GTYRAC 4 cut(s) 316, 340, 442, 964
HindII GTYRAC 4 cut(s) 316, 340, 442, 964
HindIII AAGCTT 1 cut(s) 997
HinfI GANTC 5 cut(s) 92, 107, 709, 1111, 1216
HpaI GTTAAC 1 cut(s) 340
HpaII CCGG 3 cut(s) 189, 959, 1402
HphI GGTGA 5 cut(s) 139, 178, 749, 815, 1225
Hpy166II GTNNAC 6 cut(s) 116, 316, 340, 442, 964, 1144
Hpy188I TCNGA 9 cut(s) 91, 106, 236, 257, 756, 949, 983, 995, 1361
Hpy188III TCNNGA 5 cut(s) 29, 488, 599, 1223, 1277
Hpy8I GTNNAC 6 cut(s) 116, 316, 340, 442, 964, 1144
Hpy99I CGWCG 4 cut(s) 170, 185, 209, 240
HpyAV CCTTC 9 cut(s) 152, 271, 352, 409, 676, 761, 1050, 1139, 1442
HpyCH4III ACNGT 2 cut(s) 879, 1081
HpyCH4IV ACGT 3 cut(s) 17, 238, 1555
HpyF10VI GCNNNNNNNGC 7 cut(s) 56, 168, 198, 207, 318, 433, 1469
HpyF3I CTNAG 5 cut(s) 575, 633, 844, 992, 1116
HpySE526I ACGT 3 cut(s) 17, 238, 1555
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 9 cut(s) 142, 602, 874, 887, 1095, 1234, 1302, 1324, 1454
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 4 cut(s) 54, 604, 1009, 1495
LguI GCTCTTC 2 cut(s) 1050, 1185
LmnI GCTCC 5 cut(s) 7, 215, 226, 242, 1476
Lsp1109I GCAGC 3 cut(s) 411, 706, 910
LweI GCATC 3 cut(s) 68, 171, 330
MabI ACCWGGT 1 cut(s) 733
MaeI CTAG 2 cut(s) 857, 1436
MaeII ACGT 3 cut(s) 17, 238, 1555
MaeIII GTNAC 3 cut(s) 184, 1156, 1213
MalI GATC 4 cut(s) 56, 606, 1011, 1497
MbiI CCGCTC 1 cut(s) 1372
MboI GATC 4 cut(s) 54, 604, 1009, 1495
MboII GAAGA 7 cut(s) 292, 752, 1014, 1037, 1202, 1384, 1579
MflI RGATCY 2 cut(s) 604, 1495
MhlI GDGCHC 2 cut(s) 231, 247
MlsI TGGCCA 1 cut(s) 930
MluCI AATT 2 cut(s) 786, 798
MluNI TGGCCA 1 cut(s) 930
MlyI GAGTC 2 cut(s) 86, 1210
MmeI TCCRAC 4 cut(s) 259, 1006, 1077, 1435
Mox20I TGGCCA 1 cut(s) 930
Mph1103I ATGCAT 1 cut(s) 1300
MscI TGGCCA 1 cut(s) 930
MseI TTAA 5 cut(s) 339, 450, 648, 785, 1179
MslI CAYNNNNRTG 1 cut(s) 1325
Msp20I TGGCCA 1 cut(s) 930
MspCI CTTAAG 1 cut(s) 647
MspI CCGG 3 cut(s) 189, 959, 1402
MspR9I CCNGG 4 cut(s) 197, 735, 894, 1481
MvaI CCWGG 4 cut(s) 197, 735, 894, 1481
MwoI GCNNNNNNNGC 7 cut(s) 56, 168, 198, 207, 318, 433, 1469
NcoI CCATGG 1 cut(s) 1091
NdeII GATC 4 cut(s) 54, 604, 1009, 1495
NlaIII CATG 9 cut(s) 142, 602, 874, 887, 1095, 1234, 1302, 1324, 1454
NlaIV GGNNCC 7 cut(s) 172, 211, 244, 290, 606, 749, 1478
NmuCI GTSAC 2 cut(s) 184, 1213
NsiI ATGCAT 1 cut(s) 1300
NspI RCATGY 1 cut(s) 1454
PagI TCATGA 1 cut(s) 598
PciSI GCTCTTC 2 cut(s) 1050, 1185
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 244, 309
PfeI GAWTC 3 cut(s) 107, 709, 1111
Pfl23II CGTACG 2 cut(s) 399, 609
PkrI GCNGC 6 cut(s) 194, 426, 721, 925, 1122, 1342
PleI GAGTC 2 cut(s) 86, 1210
PpsI GAGTC 2 cut(s) 86, 1210
Ppu21I YACGTR 1 cut(s) 18
PshAI GACNNNNGTC 1 cut(s) 483
PsiI TTATAA 1 cut(s) 69
Psp124BI GAGCTC 1 cut(s) 231
Psp6I CCWGG 4 cut(s) 195, 733, 892, 1479
PspGI CCWGG 4 cut(s) 195, 733, 892, 1479
PspLI CGTACG 2 cut(s) 399, 609
PspN4I GGNNCC 7 cut(s) 172, 211, 244, 290, 606, 749, 1478
PspPI GGNCC 6 cut(s) 288, 571, 731, 1099, 1283, 1361
PstI CTGCAG 1 cut(s) 429
PsuI RGATCY 2 cut(s) 604, 1495
RsaI GTAC 8 cut(s) 20, 43, 401, 611, 653, 849, 1035, 1368
RsaNI GTAC 8 cut(s) 19, 42, 400, 610, 652, 848, 1034, 1367
RseI CAYNNNNRTG 1 cut(s) 1325
SacI GAGCTC 1 cut(s) 231
SapI GCTCTTC 2 cut(s) 1050, 1185
SaqAI TTAA 5 cut(s) 339, 450, 648, 785, 1179
SatI GCNGC 6 cut(s) 193, 425, 720, 924, 1121, 1341
Sau3AI GATC 4 cut(s) 54, 604, 1009, 1495
Sau96I GGNCC 6 cut(s) 288, 571, 731, 1099, 1283, 1361
ScaI AGTACT 2 cut(s) 43, 849
SchI GAGTC 2 cut(s) 86, 1210
ScrFI CCNGG 4 cut(s) 197, 735, 894, 1481
SduI GDGCHC 2 cut(s) 231, 247
SexAI ACCWGGT 1 cut(s) 733
SfaNI GCATC 3 cut(s) 68, 171, 330
SfcI CTRYAG 1 cut(s) 425
SgrAI CRCCGGYG 1 cut(s) 188
SinI GGWCC 4 cut(s) 288, 731, 1099, 1361
SmiMI CAYNNNNRTG 1 cut(s) 1325
SmlI CTYRAG 2 cut(s) 647, 1038
SmoI CTYRAG 2 cut(s) 647, 1038
SpeI ACTAGT 1 cut(s) 856
Sse9I AATT 2 cut(s) 786, 798
SsiI CCGC 8 cut(s) 192, 213, 268, 374, 1121, 1341, 1370, 1411
SspMI CTAG 2 cut(s) 857, 1436
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 4 cut(s) 195, 733, 892, 1479
StyI CCWWGG 5 cut(s) 284, 307, 325, 1091, 1185
TaaI ACNGT 2 cut(s) 879, 1081
TaiI ACGT 3 cut(s) 20, 241, 1558
TaqI TCGA 5 cut(s) 165, 264, 303, 489, 501
TaqII GACCGA 1 cut(s) 1116
TasI AATT 2 cut(s) 786, 798
TatI WGTACW 3 cut(s) 41, 651, 847
TauI GCSGC 3 cut(s) 195, 1123, 1343
TfiI GAWTC 3 cut(s) 107, 709, 1111
Tru1I TTAA 5 cut(s) 339, 450, 648, 785, 1179
Tru9I TTAA 5 cut(s) 339, 450, 648, 785, 1179
TscAI CASTG 2 cut(s) 82, 993
TseFI GTSAC 2 cut(s) 184, 1213
TseI GCWGC 3 cut(s) 424, 719, 923
Tsp45I GTSAC 2 cut(s) 184, 1213
TspDTI ATGAA 2 cut(s) 967, 1014
TspGWI ACGGA 4 cut(s) 20, 417, 597, 780
TspRI CASTG 2 cut(s) 82, 993
Vha464I CTTAAG 1 cut(s) 647
VpaK11BI GGWCC 4 cut(s) 288, 731, 1099, 1361
XceI RCATGY 1 cut(s) 1454
XmiI GTMKAC 1 cut(s) 1143
XspI CTAG 2 cut(s) 857, 1436
ZrmI AGTACT 2 cut(s) 43, 849
Zsp2I ATGCAT 1 cut(s) 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.