RLG00000002552

Short-chain dehydrogenase reductase family 42E member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0012481
Physical Location & Seq
Reverse (-)
36197208 .. 36200072
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002552

Sequence Viewer

Length: 1443 bp
ATGCACCTGAGCGAAAATGAAGGCATCGACGGCAGCACTTTCGTGGTCACCGGCGGTTTGGGCTTCGTGGGCTCCGCACTCTGCTTGGAGCTTATCCGACGAGGAGCTCGTCAAGTCCGAGCCTTTGACCGCCGAACATCCTCCCCTTGGTCCCCCCATCTCCTCAACCAAGGGGTCAACTGCATCCAAGGGGATGTTGTTAACAAAAAGGATGTGGAGAAGGCTCTGCGGGGGGCAGATTGTGTGTTCCACCTAGCCTCGTACGGAATGTCGGGAAAGGAAATGCTGCAATTCGGGCGTGTTGATGATGTTAATATCAATGGAACTTGTCATATATTGGATACTTGTCTCGATTTCGGGGTCCAAAGGCTTGTGTATGTGAGCACATACAATGTTGTGTTTGGGGGAAAGGAGATTGTGAGCGGGAATGAGGCCCTAAGCTATTTTCCTATTGATAGTCATGAGGATCCATACGGCAGAAGTAAATCCATTGCTGAGCAGTTGGTTCTTAAGTACAATGCTCGTCCCTTCAGGACGAAGAATGGGAAGCGTCTGTATACCTGTGCGGTTCGTCCGGCTGCCATCTATGGACCTGGTGAAGAAAGGCACCTTCCGAGGATAGTGTCCCTAGCAAAGCTAGGCTTAATTCCGTTCAAAATTGGCGAAGCAAATGTGAAAACAGACTGGATTTATGTGGATAACCTAGTACTTGCTCTCATATTGGCGAGCATGGGACTTTTGCATGACATTCCTGGGAGAGAGAAAGAAAGACAACCCATAGCTGCTGGCCAACCATATTTTGTATCAGACGGGTCTCCAGTCAACACCTTTGAGTTCATCCGACCACTGCTCGGAAGCTTGGATTATGATCTACCAAAGGCTTCATTGTCTGTACCTCAAGCTCTTTGTTTAGGAAGGATTTTTTGGGCAGTCTATACAATATTATATCCATGGTTGGACCGATGGTGGATTCCTCAGCCACTTATCCTTCCTGCTGAAGTATACAAGGTTGGTGTTACACACTACTTCTCTTATCTTAAAGCCAAGGAAGAGCTTGGCTATGCCCCGGTGGTGACTCCTCGGGAGGGCATGGCTACTACAATCTCATACTGGCAGGAGAGGAAAAGGAAAACTCTGGACGGGCCTACAATATACGCATGGCTATTTTGTGTGATTGGCATGGGTATAGTATTTTGTGCTGCTTATTTGCCAGACATCGGACCTGTACCACTCTTTAGAGCTGTTAGCCTCTTCTTTTTCCGGTCAATGCAGATAGTGCGGATGGTTTTCCTTCTAGCCACTGCATTGCATGTTGGAGAGGCTGTTTACGCGTGGAAACTGGCAAAACGGGTTGATCCTGCTAACGCAAGTGGATGGTTCTGGCAGACCTTTGCTCTCGGGGTGTTTTCACTACGTCTTTTGTTGAAGAAAGCAAAGAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

481

Amino Acids

53.72

Weight (kDa)

9.15

Isoelectric Point (pI)

37.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 12 - 129 5.2e-07 NmrA-like family
3Beta_HSD PF01073 15 - 295 1.7e-83 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 15 - 242 1.8e-33 NAD dependent epimerase/dehydratase family
RmlD_sub_bind PF04321 15 - 175 2.8e-09 RmlD substrate binding domain
Polysacc_synt_2 PF02719 15 - 130 7.5e-09 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 16 - 241 8.6e-19 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 16 - 236 3.2e-07 Male sterility protein
NAD_binding_10 PF13460 18 - 132 5.9e-12 NAD(P)H-binding
TMEM254 PF14934 389 - 475 1.4e-29 Transmembrane protein 254
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33630
fragaria_vesca FvH4_5g26130 FvH4_5g26130
malus_domestica MD06G1004300.v1.1
prunus_persica Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1
pyrus_communis pycom06g00410
rosa_chinensis RchiOBHm_Chr7g0216661
rosa_laevigata RLG00000002552
rosa_multiflora Rmu_sc0029435.1_g000001 Rmu_ssc0000117.1_g000009
rosa_roxburghii Rroxscaffold_3G00243030
rosa_rugosa Rorug07G0161500
rosa_samantha Rh7AG301300 Rh7BG292800 Rh7CG319900 Rh7DG301900
rosa_wichuraiana Rw7G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 606
AccBSI CCGCTC 1 cut(s) 423
AccI GTMKAC 2 cut(s) 557, 1002
AccII CGCG 1 cut(s) 1331
AciI CCGC 7 cut(s) 54, 75, 130, 229, 423, 566, 1279
AclWI GGATC 3 cut(s) 461, 474, 1349
AcoI YGGCCR 1 cut(s) 787
AcuI CTGAAG 2 cut(s) 514, 1017
AfaI GTAC 5 cut(s) 263, 515, 708, 894, 1227
AfiI CCNNNNNNNGG 4 cut(s) 147, 851, 1085, 1217
AflII CTTAAG 1 cut(s) 509
AflIII ACRYGT 1 cut(s) 1329
AgsI TTSAA 2 cut(s) 655, 1426
AjnI CCWGG 2 cut(s) 592, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 907, 939
AleI CACNNNNGTG 1 cut(s) 41
AluBI AGCT 9 cut(s) 91, 107, 441, 637, 782, 858, 902, 1054, 1241
AluI AGCT 9 cut(s) 91, 107, 441, 637, 782, 858, 902, 1054, 1241
Alw21I GWGCWC 2 cut(s) 109, 386
Alw26I GTCTC 2 cut(s) 353, 819
AlwI GGATC 3 cut(s) 461, 474, 1349
Ama87I CYCGRG 2 cut(s) 1080, 1397
AoxI GGCC 3 cut(s) 432, 787, 1142
ApeKI GCWGC 5 cut(s) 33, 286, 578, 782, 1199
AspS9I GGNCC 7 cut(s) 150, 361, 433, 590, 958, 1142, 1220
AsuC2I CCSGG 1 cut(s) 1067
AsuHPI GGTGA 3 cut(s) 40, 608, 1084
AvaI CYCGRG 2 cut(s) 1080, 1397
AvaII GGWCC 5 cut(s) 150, 361, 590, 958, 1220
BalI TGGCCA 1 cut(s) 789
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 606
BanII GRGCYC 2 cut(s) 74, 109
Bbv12I GWGCWC 2 cut(s) 109, 386
BbvCI CCTCAGC 1 cut(s) 975
BbvI GCAGC 5 cut(s) 45, 273, 565, 769, 1186
BccI CCATC 5 cut(s) 165, 590, 957, 1276, 1368
BceAI ACGGC 2 cut(s) 46, 490
BcgI CGANNNNNNTGC 2 cut(s) 22, 56
BciT130I CCWGG 2 cut(s) 594, 753
BciVI GTATCC 1 cut(s) 334
BcnI CCSGG 1 cut(s) 1067
BcoDI GTCTC 2 cut(s) 353, 819
BfaI CTAG 5 cut(s) 254, 629, 638, 704, 1295
BfrI CTTAAG 1 cut(s) 509
BfuI GTATCC 1 cut(s) 334
BisI GCNGC 5 cut(s) 34, 287, 579, 783, 1200
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 5 cut(s) 35, 288, 580, 784, 1201
BmcAI AGTACT 1 cut(s) 708
Bme1390I CCNGG 3 cut(s) 594, 753, 1067
Bme18I GGWCC 5 cut(s) 150, 361, 590, 958, 1220
BmeT110I CYCGRG 2 cut(s) 1080, 1397
BmgT120I GGNCC 7 cut(s) 150, 361, 433, 590, 958, 1142, 1220
BmiI GGNNCC 5 cut(s) 73, 152, 362, 468, 608
BmrFI CCNGG 3 cut(s) 594, 753, 1067
BmsI GCATC 2 cut(s) 33, 192
BpmI CTGGAG 1 cut(s) 801
Bpu10I CCTNAGC 3 cut(s) 8, 437, 975
Bpu1102I GCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 882
BpuMI CCSGG 1 cut(s) 1067
BsaBI GATNNNNATC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 819
BsaJI CCNNGG 9 cut(s) 146, 169, 187, 614, 752, 950, 1044, 1065, 1079
BsaWI WCCGGW 1 cut(s) 1260
BsaXI ACNNNNNCTCC 2 cut(s) 1308, 1338
Bsc4I CCNNNNNNNGG 4 cut(s) 147, 851, 1085, 1217
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 4 cut(s) 689, 818, 1115, 1344
Bse3DI GCAATG 2 cut(s) 489, 1304
Bse8I GATNNNNATC 1 cut(s) 867
BseBI CCWGG 2 cut(s) 594, 753
BseDI CCNNGG 9 cut(s) 146, 169, 187, 614, 752, 950, 1044, 1065, 1079
BseGI GGATG 7 cut(s) 137, 183, 199, 217, 837, 1287, 1379
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 4 cut(s) 147, 851, 1085, 1217
BseMI GCAATG 2 cut(s) 489, 1304
BseMII CTCAG 2 cut(s) 486, 989
BseNI ACTGG 4 cut(s) 689, 818, 1115, 1344
BseRI GAGGAG 3 cut(s) 117, 152, 1068
BseXI GCAGC 5 cut(s) 45, 273, 565, 769, 1186
Bsh1236I CGCG 1 cut(s) 1331
BshFI GGCC 3 cut(s) 434, 789, 1144
BshNI GGYRCC 1 cut(s) 606
BsiHKAI GWGCWC 2 cut(s) 109, 386
BsiHKCI CYCGRG 2 cut(s) 1080, 1397
BsiSI CCGG 4 cut(s) 51, 575, 1067, 1261
BsiWI CGTACG 1 cut(s) 261
BslFI GGGAC 4 cut(s) 136, 510, 610, 747
BslI CCNNNNNNNGG 4 cut(s) 147, 851, 1085, 1217
BsmAI GTCTC 2 cut(s) 353, 819
BsmFI GGGAC 4 cut(s) 136, 510, 610, 747
BsnI GGCC 3 cut(s) 434, 789, 1144
Bso31I GGTCTC 1 cut(s) 819
BsoBI CYCGRG 2 cut(s) 1080, 1397
Bsp1286I GDGCHC 3 cut(s) 74, 109, 386
Bsp143I GATC 3 cut(s) 466, 868, 1354
Bsp1720I GCTNAGC 1 cut(s) 495
Bsp19I CCATGG 1 cut(s) 950
BspACI CCGC 7 cut(s) 54, 75, 130, 229, 423, 566, 1279
BspANI GGCC 3 cut(s) 434, 789, 1144
BspCNI CTCAG 2 cut(s) 487, 988
BspFNI CGCG 1 cut(s) 1331
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 5 cut(s) 73, 152, 362, 468, 608
BspPI GGATC 3 cut(s) 461, 474, 1349
BspQI GCTCTTC 1 cut(s) 1044
BspT107I GGYRCC 1 cut(s) 606
BspTI CTTAAG 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 819
BsrBI CCGCTC 1 cut(s) 423
BsrDI GCAATG 2 cut(s) 489, 1304
BsrFI RCCGGY 1 cut(s) 50
BsrI ACTGG 4 cut(s) 689, 818, 1115, 1344
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 9 cut(s) 146, 169, 187, 614, 752, 950, 1044, 1065, 1079
BssMI GATC 3 cut(s) 466, 868, 1354
BssNAI GTATAC 2 cut(s) 558, 1003
BssT1I CCWWGG 5 cut(s) 146, 169, 187, 950, 1044
Bst1107I GTATAC 2 cut(s) 558, 1003
Bst2UI CCWGG 2 cut(s) 594, 753
Bst6I CTCTTC 2 cut(s) 1044, 1256
BstAFI CTTAAG 1 cut(s) 509
BstAPI GCANNNNNTGC 1 cut(s) 1276
BstC8I GCNNGC 2 cut(s) 727, 787
BstDEI CTNAG 4 cut(s) 8, 437, 495, 975
BstDSI CCRYGG 1 cut(s) 950
BstEII GGTNACC 1 cut(s) 46
BstF5I GGATG 7 cut(s) 137, 183, 199, 217, 837, 1287, 1379
BstFNI CGCG 1 cut(s) 1331
BstKTI GATC 3 cut(s) 469, 871, 1357
BstMAI GTCTC 2 cut(s) 353, 819
BstMBI GATC 3 cut(s) 466, 868, 1354
BstMWI GCNNNNNNNGC 6 cut(s) 30, 60, 69, 295, 1276, 1328
BstNI CCWGG 2 cut(s) 594, 753
BstNSI RCATGY 1 cut(s) 1313
BstPI GGTNACC 1 cut(s) 46
BstSCI CCNGG 3 cut(s) 592, 751, 1065
BstUI CGCG 1 cut(s) 1331
BstV1I GCAGC 5 cut(s) 45, 273, 565, 769, 1186
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BstZ17I GTATAC 2 cut(s) 558, 1003
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 3 cut(s) 434, 789, 1144
BtgI CCRYGG 1 cut(s) 950
BtsCI GGATG 7 cut(s) 137, 183, 199, 217, 837, 1287, 1379
BtsI GCAGTG 2 cut(s) 845, 1299
BtsIMutI CAGTG 2 cut(s) 845, 1299
Cac8I GCNNGC 2 cut(s) 727, 787
CciI TCATGA 1 cut(s) 460
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 7 cut(s) 150, 361, 433, 590, 958, 1142, 1220
CseI GACGC 1 cut(s) 539
CsiI ACCWGGT 1 cut(s) 592
Csp6I GTAC 5 cut(s) 262, 514, 707, 893, 1226
CviAII CATG 8 cut(s) 461, 730, 743, 951, 1090, 1158, 1180, 1310
CviQI GTAC 5 cut(s) 262, 514, 707, 893, 1226
DdeI CTNAG 4 cut(s) 8, 437, 495, 975
DpnI GATC 3 cut(s) 468, 870, 1356
DpnII GATC 3 cut(s) 466, 868, 1354
EaeI YGGCCR 1 cut(s) 787
Eam1104I CTCTTC 2 cut(s) 1044, 1256
EarI CTCTTC 2 cut(s) 1044, 1256
Ecl136II GAGCTC 1 cut(s) 107
Eco130I CCWWGG 5 cut(s) 146, 169, 187, 950, 1044
Eco24I GRGCYC 2 cut(s) 74, 109
Eco31I GGTCTC 1 cut(s) 819
Eco47I GGWCC 5 cut(s) 150, 361, 590, 958, 1220
Eco53kI GAGCTC 1 cut(s) 107
Eco57I CTGAAG 2 cut(s) 514, 1017
Eco88I CYCGRG 2 cut(s) 1080, 1397
Eco91I GGTNACC 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 433
EcoO65I GGTNACC 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 592, 751
EcoT14I CCWWGG 5 cut(s) 146, 169, 187, 950, 1044
EcoT38I GRGCYC 2 cut(s) 74, 109
ErhI CCWWGG 5 cut(s) 146, 169, 187, 950, 1044
FaeI CATG 8 cut(s) 464, 733, 746, 954, 1093, 1161, 1183, 1313
FalI AAGNNNNNCTT 2 cut(s) 626, 658
FaqI GGGAC 4 cut(s) 136, 510, 610, 747
FatI CATG 8 cut(s) 460, 729, 742, 950, 1089, 1157, 1179, 1309
FauI CCCGC 2 cut(s) 222, 416
FblI GTMKAC 2 cut(s) 557, 1002
Fnu4HI GCNGC 5 cut(s) 34, 287, 579, 783, 1200
FokI GGATG 7 cut(s) 124, 170, 206, 224, 824, 1294, 1386
FriOI GRGCYC 2 cut(s) 74, 109
Fsp4HI GCNGC 5 cut(s) 34, 287, 579, 783, 1200
FspBI CTAG 5 cut(s) 254, 629, 638, 704, 1295
GluI GCNGC 5 cut(s) 34, 287, 579, 783, 1200
GsuI CTGGAG 1 cut(s) 801
HaeIII GGCC 3 cut(s) 434, 789, 1144
HapII CCGG 4 cut(s) 51, 575, 1067, 1261
HgaI GACGC 1 cut(s) 539
Hin1II CATG 8 cut(s) 464, 733, 746, 954, 1093, 1161, 1183, 1313
HincII GTYRAC 3 cut(s) 178, 202, 823
HindII GTYRAC 3 cut(s) 178, 202, 823
HindIII AAGCTT 1 cut(s) 856
HinfI GANTC 2 cut(s) 970, 1075
HpaI GTTAAC 1 cut(s) 202
HpaII CCGG 4 cut(s) 51, 575, 1067, 1261
HphI GGTGA 3 cut(s) 40, 608, 1084
Hpy166II GTNNAC 6 cut(s) 178, 202, 558, 823, 1003, 1327
Hpy188I TCNGA 7 cut(s) 98, 119, 615, 808, 842, 854, 1220
Hpy188III TCNNGA 6 cut(s) 273, 350, 461, 532, 1082, 1136
Hpy8I GTNNAC 6 cut(s) 178, 202, 558, 823, 1003, 1327
Hpy99I CGWCG 2 cut(s) 32, 102
HpyAV CCTTC 7 cut(s) 14, 214, 538, 620, 909, 998, 1301
HpyCH4IV ACGT 1 cut(s) 1414
HpyCH4V TGCA 7 cut(s) 4, 183, 289, 742, 1270, 1304, 1309
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 60, 69, 295, 1276, 1328
HpyF3I CTNAG 4 cut(s) 8, 437, 495, 975
HpySE526I ACGT 1 cut(s) 1414
Hsp92II CATG 8 cut(s) 464, 733, 746, 954, 1093, 1161, 1183, 1313
KspAI GTTAAC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 466, 868, 1354
LguI GCTCTTC 1 cut(s) 1044
LmnI GCTCC 3 cut(s) 77, 88, 104
Lsp1109I GCAGC 5 cut(s) 45, 273, 565, 769, 1186
LweI GCATC 2 cut(s) 33, 192
MabI ACCWGGT 1 cut(s) 592
MaeI CTAG 5 cut(s) 254, 629, 638, 704, 1295
MaeII ACGT 1 cut(s) 1414
MaeIII GTNAC 3 cut(s) 46, 1015, 1072
MalI GATC 3 cut(s) 468, 870, 1356
MbiI CCGCTC 1 cut(s) 423
MboI GATC 3 cut(s) 466, 868, 1354
MboII GAAGA 5 cut(s) 550, 611, 1061, 1243, 1438
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 3 cut(s) 74, 109, 386
MlsI TGGCCA 1 cut(s) 789
MluCI AATT 3 cut(s) 290, 645, 657
MluI ACGCGT 1 cut(s) 1329
MluNI TGGCCA 1 cut(s) 789
MlyI GAGTC 1 cut(s) 1069
MmeI TCCRAC 4 cut(s) 121, 865, 936, 1294
Mox20I TGGCCA 1 cut(s) 789
MscI TGGCCA 1 cut(s) 789
MseI TTAA 5 cut(s) 201, 312, 510, 644, 1038
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 789
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 4 cut(s) 51, 575, 1067, 1261
MspR9I CCNGG 3 cut(s) 594, 753, 1067
MvaI CCWGG 2 cut(s) 594, 753
MvnI CGCG 1 cut(s) 1331
MwoI GCNNNNNNNGC 6 cut(s) 30, 60, 69, 295, 1276, 1328
NciI CCSGG 1 cut(s) 1067
NcoI CCATGG 1 cut(s) 950
NdeII GATC 3 cut(s) 466, 868, 1354
NlaIII CATG 8 cut(s) 464, 733, 746, 954, 1093, 1161, 1183, 1313
NlaIV GGNNCC 5 cut(s) 73, 152, 362, 468, 608
NmuCI GTSAC 2 cut(s) 46, 1072
NspI RCATGY 1 cut(s) 1313
OliI CACNNNNGTG 1 cut(s) 41
PagI TCATGA 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 1044
PcsI WCGNNNNNNNCGW 2 cut(s) 106, 115
PfeI GAWTC 1 cut(s) 970
Pfl23II CGTACG 1 cut(s) 261
PkrI GCNGC 5 cut(s) 35, 288, 580, 784, 1201
PleI GAGTC 1 cut(s) 1069
PpsI GAGTC 1 cut(s) 1069
Psp124BI GAGCTC 1 cut(s) 109
Psp6I CCWGG 2 cut(s) 592, 751
PspEI GGTNACC 1 cut(s) 46
PspGI CCWGG 2 cut(s) 592, 751
PspLI CGTACG 1 cut(s) 261
PspN4I GGNNCC 5 cut(s) 73, 152, 362, 468, 608
PspPI GGNCC 7 cut(s) 150, 361, 433, 590, 958, 1142, 1220
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 5 cut(s) 263, 515, 708, 894, 1227
RsaNI GTAC 5 cut(s) 262, 514, 707, 893, 1226
RseI CAYNNNNRTG 1 cut(s) 41
SacI GAGCTC 1 cut(s) 109
SapI GCTCTTC 1 cut(s) 1044
SaqAI TTAA 5 cut(s) 201, 312, 510, 644, 1038
SatI GCNGC 5 cut(s) 34, 287, 579, 783, 1200
Sau3AI GATC 3 cut(s) 466, 868, 1354
Sau96I GGNCC 7 cut(s) 150, 361, 433, 590, 958, 1142, 1220
ScaI AGTACT 1 cut(s) 708
SchI GAGTC 1 cut(s) 1069
ScrFI CCNGG 3 cut(s) 594, 753, 1067
SduI GDGCHC 3 cut(s) 74, 109, 386
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 2 cut(s) 33, 192
SgrAI CRCCGGYG 1 cut(s) 50
SinI GGWCC 5 cut(s) 150, 361, 590, 958, 1220
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 2 cut(s) 509, 897
SmoI CTYRAG 2 cut(s) 509, 897
Sse9I AATT 3 cut(s) 290, 645, 657
SsiI CCGC 7 cut(s) 54, 75, 130, 229, 423, 566, 1279
SspI AATATT 1 cut(s) 942
SspMI CTAG 5 cut(s) 254, 629, 638, 704, 1295
SstI GAGCTC 1 cut(s) 109
StyD4I CCNGG 3 cut(s) 592, 751, 1065
StyI CCWWGG 5 cut(s) 146, 169, 187, 950, 1044
TaiI ACGT 1 cut(s) 1417
TaqI TCGA 2 cut(s) 27, 351
TaqII GACCGA 1 cut(s) 975
TasI AATT 3 cut(s) 290, 645, 657
TatI WGTACW 2 cut(s) 513, 706
TfiI GAWTC 1 cut(s) 970
Tru1I TTAA 5 cut(s) 201, 312, 510, 644, 1038
Tru9I TTAA 5 cut(s) 201, 312, 510, 644, 1038
TscAI CASTG 2 cut(s) 852, 1306
TseFI GTSAC 2 cut(s) 46, 1072
TseI GCWGC 5 cut(s) 33, 286, 578, 782, 1199
Tsp45I GTSAC 2 cut(s) 46, 1072
TspDTI ATGAA 3 cut(s) 33, 826, 873
TspGWI ACGGA 2 cut(s) 279, 639
TspRI CASTG 2 cut(s) 852, 1306
Vha464I CTTAAG 1 cut(s) 509
VpaK11BI GGWCC 5 cut(s) 150, 361, 590, 958, 1220
XceI RCATGY 1 cut(s) 1313
XmiI GTMKAC 2 cut(s) 557, 1002
XspI CTAG 5 cut(s) 254, 629, 638, 704, 1295
ZrmI AGTACT 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.