Rh7DG301900

Short-chain dehydrogenase reductase family 42E member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0012481
Physical Location & Seq
Forward (+)
34695604 .. 34698420
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG301900.1

Sequence Viewer

Length: 1155 bp
ATGCATCTGAGCGAAAATGAAGGCATCGACGGCAGCACTTTCGTCGTGACCGGCGGTTTGGGCTTCGTGGGCTCCGCACTCTGCTTGGAGCTTATGCGACGAGGAGCCCGTCAAGTCCGAGCCTTTGACCGCCGAACCTCCTCCCCTTGGTCCGCCCATCTCCTCAACCAAGGGGTCAACTGCATCCAAGGGGATGTTGTTAACAAAAAGGATGTGGAGAAGGCTCTGCGGGGGGCCGATTGTGTGTTCCACCTAGCGTCGTACGGAATGTCGGGAAAGGAAATGCTGCAATTCGGGCGTGTTGATGATGTTAATATCAATGGAACTTGTCGTATATTGGATACTTGTCTCGATTTCGGGGTCGAAAGGCTTGTGTATGTGAGCACATACAATGTTGTGTTTGGGGGAAAGGAGATTGTGAGTGGGAATGAGGCCCTAAGCTATTTTCCTATTGATAGTCATGAGGATCCATACGGCAGAAGTAAATCCATTGCTGAGCAGTTGGTTCTTAAGTACAATGCTCGTCCTTTCAGGACGAAGAATGGGAAGTGTCTGTATACCTGTGCGGTTCGTCCAGCTGCTATCTATGGACCTGGTGAAGAAAGGCACCTTCCGAGGATTGTGTCCCTAGCAAAGTTAGGCTTAATTCCATTCAAAATTGGCGAAGCAAATGTGAAAACAGACTGGATTTATGTGGATAACCTAGTACTTGCTCTCATATTGGCGAGCATGGGCCTTTTGCTTGACATTCCTGGGAGAGAGAAAGAAAGACAACCCATAGCTGCTGGCCAACCATATTTTGTATCGGACGGGTCTCCGGTCAACACCTTTGAGTTCATCCGACCACTGCTCAGAAGCTTGGATTATGATCTACCAAAGGCTTCATTGTCTGTACCTCAAGCTCTTCGTTTAGGAAGGATTTTTTGGGCAGTCTATACAATATTATATCCATGGTTGGACCGATGGTGGATTCCTCAGCCGCTTATCCTTCCTGCTGAAGTATACAAGGTTTGCTTGTACCCCTCTCCTTCTCTTCTAACTTTTTGTTTTTGGCTCAATATACTTGGTTTTGTTGCATCGGGCAGTTCTATCTTCTTCCACCAATTTTTTCTCCTTGCAATTAAAACAGAAATGTCTGCAATCAAGTGTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

384

Amino Acids

42.89

Weight (kDa)

8.25

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 12 - 127 6.6e-07 NmrA-like family
KR PF08659 13 - 80 7.4e-06 KR domain
3Beta_HSD PF01073 15 - 295 5.5e-83 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 15 - 242 9.3e-35 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 15 - 130 2e-09 Polysaccharide biosynthesis protein
RmlD_sub_bind PF04321 15 - 175 1.2e-09 RmlD substrate binding domain
GDP_Man_Dehyd PF16363 16 - 241 1.5e-19 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 16 - 236 2.1e-07 Male sterility protein
NAD_binding_10 PF13460 18 - 132 4.2e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33630
fragaria_vesca FvH4_5g26130 FvH4_5g26130
malus_domestica MD06G1004300.v1.1
prunus_persica Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1
pyrus_communis pycom06g00410
rosa_chinensis RchiOBHm_Chr7g0216661
rosa_laevigata RLG00000002552
rosa_multiflora Rmu_sc0029435.1_g000001 Rmu_ssc0000117.1_g000009
rosa_roxburghii Rroxscaffold_3G00243030
rosa_rugosa Rorug07G0161500
rosa_samantha Rh7AG301300 Rh7BG292800 Rh7CG319900 Rh7DG301900
rosa_wichuraiana Rw7G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 606
AccI GTMKAC 2 cut(s) 557, 1002
AciI CCGC 7 cut(s) 54, 75, 130, 153, 229, 566, 980
AclWI GGATC 2 cut(s) 461, 474
AcoI YGGCCR 1 cut(s) 787
AcuI CTGAAG 1 cut(s) 1017
AfaI GTAC 5 cut(s) 263, 515, 708, 894, 1019
AfiI CCNNNNNNNGG 1 cut(s) 147
AflII CTTAAG 1 cut(s) 509
AgsI TTSAA 1 cut(s) 655
AjnI CCWGG 2 cut(s) 592, 751
AjuI GAANNNNNNNTTGG 2 cut(s) 907, 939
AluBI AGCT 6 cut(s) 91, 441, 578, 782, 858, 902
AluI AGCT 6 cut(s) 91, 441, 578, 782, 858, 902
Alw21I GWGCWC 1 cut(s) 386
Alw26I GTCTC 2 cut(s) 353, 819
AlwI GGATC 2 cut(s) 461, 474
AoxI GGCC 4 cut(s) 234, 432, 733, 787
ApeKI GCWGC 4 cut(s) 33, 286, 578, 782
ArsI GACNNNNNNTTYG 2 cut(s) 40, 72
AspS9I GGNCC 6 cut(s) 150, 234, 433, 590, 733, 958
AsuHPI GGTGA 1 cut(s) 608
AvaII GGWCC 3 cut(s) 150, 590, 958
BalI TGGCCA 1 cut(s) 789
BamHI GGATCC 1 cut(s) 466
BanI GGYRCC 1 cut(s) 606
BanII GRGCYC 2 cut(s) 74, 109
Bbv12I GWGCWC 1 cut(s) 386
BbvCI CCTCAGC 1 cut(s) 975
BbvI GCAGC 4 cut(s) 45, 273, 565, 769
BccI CCATC 2 cut(s) 165, 957
BceAI ACGGC 2 cut(s) 46, 490
BcgI CGANNNNNNTGC 4 cut(s) 22, 25, 56, 59
BciT130I CCWGG 2 cut(s) 594, 753
BciVI GTATCC 1 cut(s) 334
BcoDI GTCTC 2 cut(s) 353, 819
BfaI CTAG 3 cut(s) 254, 629, 704
BfrI CTTAAG 1 cut(s) 509
BfuI GTATCC 1 cut(s) 334
BisI GCNGC 5 cut(s) 34, 287, 579, 783, 980
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 5 cut(s) 35, 288, 580, 784, 981
BmcAI AGTACT 1 cut(s) 708
Bme1390I CCNGG 2 cut(s) 594, 753
Bme18I GGWCC 3 cut(s) 150, 590, 958
BmgT120I GGNCC 6 cut(s) 150, 234, 433, 590, 733, 958
BmiI GGNNCC 5 cut(s) 73, 106, 235, 468, 608
BmrFI CCNGG 2 cut(s) 594, 753
BmsI GCATC 4 cut(s) 13, 33, 192, 1085
Bpu10I CCTNAGC 2 cut(s) 437, 975
Bpu1102I GCTNAGC 1 cut(s) 495
BpuEI CTTGAG 1 cut(s) 882
BsaBI GATNNNNATC 1 cut(s) 867
BsaI GGTCTC 1 cut(s) 819
BsaJI CCNNGG 6 cut(s) 146, 169, 187, 614, 752, 950
BsaWI WCCGGW 1 cut(s) 817
Bsc4I CCNNNNNNNGG 1 cut(s) 147
Bse118I RCCGGY 1 cut(s) 50
Bse1I ACTGG 1 cut(s) 689
Bse3DI GCAATG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 867
BseBI CCWGG 2 cut(s) 594, 753
BseDI CCNNGG 6 cut(s) 146, 169, 187, 614, 752, 950
BseGI GGATG 4 cut(s) 183, 199, 217, 837
BseJI GATNNNNATC 1 cut(s) 867
BseLI CCNNNNNNNGG 1 cut(s) 147
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 3 cut(s) 486, 865, 989
BseNI ACTGG 1 cut(s) 689
BseRI GAGGAG 3 cut(s) 117, 130, 152
BseXI GCAGC 4 cut(s) 45, 273, 565, 769
BshFI GGCC 4 cut(s) 236, 434, 735, 789
BshNI GGYRCC 1 cut(s) 606
BsiHKAI GWGCWC 1 cut(s) 386
BsiSI CCGG 2 cut(s) 51, 818
BsiWI CGTACG 1 cut(s) 261
BslFI GGGAC 1 cut(s) 610
BslI CCNNNNNNNGG 1 cut(s) 147
BsmAI GTCTC 2 cut(s) 353, 819
BsmFI GGGAC 1 cut(s) 610
BsnI GGCC 4 cut(s) 236, 434, 735, 789
Bso31I GGTCTC 1 cut(s) 819
Bsp1286I GDGCHC 3 cut(s) 74, 109, 386
Bsp143I GATC 2 cut(s) 466, 868
Bsp1720I GCTNAGC 1 cut(s) 495
Bsp19I CCATGG 1 cut(s) 950
BspACI CCGC 7 cut(s) 54, 75, 130, 153, 229, 566, 980
BspANI GGCC 4 cut(s) 236, 434, 735, 789
BspCNI CTCAG 3 cut(s) 487, 864, 988
BspHI TCATGA 1 cut(s) 460
BspLI GGNNCC 5 cut(s) 73, 106, 235, 468, 608
BspPI GGATC 2 cut(s) 461, 474
BspQI GCTCTTC 1 cut(s) 909
BspT107I GGYRCC 1 cut(s) 606
BspTI CTTAAG 1 cut(s) 509
BspTNI GGTCTC 1 cut(s) 819
BsrDI GCAATG 1 cut(s) 489
BsrFI RCCGGY 1 cut(s) 50
BsrI ACTGG 1 cut(s) 689
BssAI RCCGGY 1 cut(s) 50
BssECI CCNNGG 6 cut(s) 146, 169, 187, 614, 752, 950
BssMI GATC 2 cut(s) 466, 868
BssNAI GTATAC 2 cut(s) 558, 1003
BssT1I CCWWGG 4 cut(s) 146, 169, 187, 950
Bst1107I GTATAC 2 cut(s) 558, 1003
Bst2UI CCWGG 2 cut(s) 594, 753
Bst6I CTCTTC 2 cut(s) 909, 1038
BstAFI CTTAAG 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 727, 787
BstDEI CTNAG 5 cut(s) 8, 437, 495, 851, 975
BstDSI CCRYGG 1 cut(s) 950
BstF5I GGATG 4 cut(s) 183, 199, 217, 837
BstKTI GATC 2 cut(s) 469, 871
BstMAI GTCTC 2 cut(s) 353, 819
BstMBI GATC 2 cut(s) 466, 868
BstMWI GCNNNNNNNGC 4 cut(s) 30, 60, 69, 295
BstNI CCWGG 2 cut(s) 594, 753
BstSCI CCNGG 2 cut(s) 592, 751
BstV1I GCAGC 4 cut(s) 45, 273, 565, 769
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BstZ17I GTATAC 2 cut(s) 558, 1003
BsuI GTATCC 1 cut(s) 334
BsuRI GGCC 4 cut(s) 236, 434, 735, 789
BtgI CCRYGG 1 cut(s) 950
BtsCI GGATG 4 cut(s) 183, 199, 217, 837
BtsI GCAGTG 1 cut(s) 845
BtsIMutI CAGTG 1 cut(s) 845
Cac8I GCNNGC 2 cut(s) 727, 787
CciI TCATGA 1 cut(s) 460
Cfr10I RCCGGY 1 cut(s) 50
Cfr13I GGNCC 6 cut(s) 150, 234, 433, 590, 733, 958
CseI GACGC 1 cut(s) 246
CsiI ACCWGGT 1 cut(s) 592
Csp6I GTAC 5 cut(s) 262, 514, 707, 893, 1018
CviAII CATG 3 cut(s) 461, 730, 951
CviQI GTAC 5 cut(s) 262, 514, 707, 893, 1018
DdeI CTNAG 5 cut(s) 8, 437, 495, 851, 975
DpnI GATC 2 cut(s) 468, 870
DpnII GATC 2 cut(s) 466, 868
EaeI YGGCCR 1 cut(s) 787
Eam1104I CTCTTC 2 cut(s) 909, 1038
EarI CTCTTC 2 cut(s) 909, 1038
EciI GGCGGA 1 cut(s) 142
Eco130I CCWWGG 4 cut(s) 146, 169, 187, 950
Eco24I GRGCYC 2 cut(s) 74, 109
Eco31I GGTCTC 1 cut(s) 819
Eco47I GGWCC 3 cut(s) 150, 590, 958
Eco57I CTGAAG 1 cut(s) 1017
EcoO109I RGGNCCY 1 cut(s) 433
EcoRII CCWGG 2 cut(s) 592, 751
EcoT14I CCWWGG 4 cut(s) 146, 169, 187, 950
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 74, 109
ErhI CCWWGG 4 cut(s) 146, 169, 187, 950
FaeI CATG 3 cut(s) 464, 733, 954
FalI AAGNNNNNCTT 4 cut(s) 626, 658, 998, 1030
FaqI GGGAC 1 cut(s) 610
FatI CATG 3 cut(s) 460, 729, 950
FauI CCCGC 1 cut(s) 222
FblI GTMKAC 2 cut(s) 557, 1002
Fnu4HI GCNGC 5 cut(s) 34, 287, 579, 783, 980
FokI GGATG 4 cut(s) 170, 206, 224, 824
FriOI GRGCYC 2 cut(s) 74, 109
Fsp4HI GCNGC 5 cut(s) 34, 287, 579, 783, 980
FspBI CTAG 3 cut(s) 254, 629, 704
GluI GCNGC 5 cut(s) 34, 287, 579, 783, 980
HaeIII GGCC 4 cut(s) 236, 434, 735, 789
HapII CCGG 2 cut(s) 51, 818
HgaI GACGC 1 cut(s) 246
Hin1II CATG 3 cut(s) 464, 733, 954
HincII GTYRAC 3 cut(s) 178, 202, 823
HindII GTYRAC 3 cut(s) 178, 202, 823
HindIII AAGCTT 1 cut(s) 856
HinfI GANTC 1 cut(s) 970
HpaI GTTAAC 1 cut(s) 202
HpaII CCGG 2 cut(s) 51, 818
HphI GGTGA 1 cut(s) 608
Hpy166II GTNNAC 5 cut(s) 178, 202, 558, 823, 1003
Hpy188I TCNGA 6 cut(s) 9, 119, 615, 808, 842, 854
Hpy188III TCNNGA 5 cut(s) 46, 273, 350, 461, 532
Hpy8I GTNNAC 5 cut(s) 178, 202, 558, 823, 1003
Hpy99I CGWCG 4 cut(s) 32, 47, 102, 262
HpyAV CCTTC 6 cut(s) 14, 214, 620, 909, 998, 1038
HpyCH4V TGCA 6 cut(s) 4, 183, 289, 1076, 1118, 1139
HpyF10VI GCNNNNNNNGC 4 cut(s) 30, 60, 69, 295
HpyF3I CTNAG 5 cut(s) 8, 437, 495, 851, 975
Hsp92II CATG 3 cut(s) 464, 733, 954
KspAI GTTAAC 1 cut(s) 202
Kzo9I GATC 2 cut(s) 466, 868
LguI GCTCTTC 1 cut(s) 909
LmnI GCTCC 3 cut(s) 77, 88, 104
Lsp1109I GCAGC 4 cut(s) 45, 273, 565, 769
LweI GCATC 4 cut(s) 13, 33, 192, 1085
MabI ACCWGGT 1 cut(s) 592
MaeI CTAG 3 cut(s) 254, 629, 704
MaeIII GTNAC 1 cut(s) 46
MalI GATC 2 cut(s) 468, 870
MboI GATC 2 cut(s) 466, 868
MboII GAAGA 6 cut(s) 550, 611, 896, 1025, 1084, 1087
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 3 cut(s) 74, 109, 386
MlsI TGGCCA 1 cut(s) 789
MluCI AATT 5 cut(s) 290, 645, 657, 1103, 1119
MluNI TGGCCA 1 cut(s) 789
MmeI TCCRAC 2 cut(s) 865, 936
Mox20I TGGCCA 1 cut(s) 789
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 789
MseI TTAA 5 cut(s) 201, 312, 510, 644, 1122
Msp20I TGGCCA 1 cut(s) 789
MspA1I CMGCKG 1 cut(s) 578
MspCI CTTAAG 1 cut(s) 509
MspI CCGG 2 cut(s) 51, 818
MspR9I CCNGG 2 cut(s) 594, 753
MvaI CCWGG 2 cut(s) 594, 753
MwoI GCNNNNNNNGC 4 cut(s) 30, 60, 69, 295
NcoI CCATGG 1 cut(s) 950
NdeII GATC 2 cut(s) 466, 868
NlaIII CATG 3 cut(s) 464, 733, 954
NlaIV GGNNCC 5 cut(s) 73, 106, 235, 468, 608
NmuCI GTSAC 1 cut(s) 46
NsiI ATGCAT 1 cut(s) 6
PagI TCATGA 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 909
PcsI WCGNNNNNNNCGW 1 cut(s) 106
PfeI GAWTC 1 cut(s) 970
Pfl23II CGTACG 1 cut(s) 261
PkrI GCNGC 5 cut(s) 35, 288, 580, 784, 981
Psp6I CCWGG 2 cut(s) 592, 751
PspGI CCWGG 2 cut(s) 592, 751
PspLI CGTACG 1 cut(s) 261
PspN4I GGNNCC 5 cut(s) 73, 106, 235, 468, 608
PspPI GGNCC 6 cut(s) 150, 234, 433, 590, 733, 958
PsuI RGATCY 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 5 cut(s) 263, 515, 708, 894, 1019
RsaNI GTAC 5 cut(s) 262, 514, 707, 893, 1018
SapI GCTCTTC 1 cut(s) 909
SaqAI TTAA 5 cut(s) 201, 312, 510, 644, 1122
SatI GCNGC 5 cut(s) 34, 287, 579, 783, 980
Sau3AI GATC 2 cut(s) 466, 868
Sau96I GGNCC 6 cut(s) 150, 234, 433, 590, 733, 958
ScaI AGTACT 1 cut(s) 708
ScrFI CCNGG 2 cut(s) 594, 753
SduI GDGCHC 3 cut(s) 74, 109, 386
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 4 cut(s) 13, 33, 192, 1085
SinI GGWCC 3 cut(s) 150, 590, 958
SmlI CTYRAG 2 cut(s) 509, 897
SmoI CTYRAG 2 cut(s) 509, 897
Sse9I AATT 5 cut(s) 290, 645, 657, 1103, 1119
SsiI CCGC 7 cut(s) 54, 75, 130, 153, 229, 566, 980
SspI AATATT 1 cut(s) 942
SspMI CTAG 3 cut(s) 254, 629, 704
StyD4I CCNGG 2 cut(s) 592, 751
StyI CCWWGG 4 cut(s) 146, 169, 187, 950
TaqI TCGA 3 cut(s) 27, 351, 363
TaqII GACCGA 1 cut(s) 975
TasI AATT 5 cut(s) 290, 645, 657, 1103, 1119
TatI WGTACW 2 cut(s) 513, 706
TauI GCSGC 1 cut(s) 982
TfiI GAWTC 1 cut(s) 970
Tru1I TTAA 5 cut(s) 201, 312, 510, 644, 1122
Tru9I TTAA 5 cut(s) 201, 312, 510, 644, 1122
TscAI CASTG 1 cut(s) 852
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 4 cut(s) 33, 286, 578, 782
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 3 cut(s) 33, 826, 873
TspGWI ACGGA 1 cut(s) 279
TspRI CASTG 1 cut(s) 852
Vha464I CTTAAG 1 cut(s) 509
VpaK11BI GGWCC 3 cut(s) 150, 590, 958
XmiI GTMKAC 2 cut(s) 557, 1002
XspI CTAG 3 cut(s) 254, 629, 704
ZrmI AGTACT 1 cut(s) 708
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.