MD06G1004300.v1.1

Short-chain dehydrogenase reductase family 42E member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012481
Physical Location & Seq
Reverse (-)
489412 .. 492610
3199 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1004300.v1.1.491

Sequence Viewer

Length: 1455 bp
ATGCACCTGAGCGAAAACGAAGGCATAGAGGGCAACACTTTTGTGGTCAGCGGTGGGTTGGGCTTCGTAGGCTCCGCACTCTGCTTGGAGCTGCTCCGGAGAGGTGCTCACCAAGTCCGAGCCTTTGATCTCCGCACAGCCTCTCCTTGGTCCGATCGCCTCCGCCAGCACGGCGTCCACTGCGTCCAAGGGGATGTAACCCAGAAAAAGGATGTTGAAAAAGCTCTGCGGGGAGCAGACTGTGTGTTCCACCTTGCTTCGTATGGCATGTCAGGAAAAGAAATGCTGCAGTTCGGTCGTGTCGATGAGGTCAACATAAATGGAACCTGCCATATCTTAGACACGTGTCTTGAGTTCGGGATTCAAAGGCTTGTTTATGTGAGCACATATAATGTTGTGTTTGGGGGACAGGAGATTGTCAATGGGAACGAGGCCTTGCGCTATTACCCTATCGACAACCATGTCGATCCATATGGCCGGAGTAAATCAATTGCCGAGCAGTTGGTCTTAAAGTACAATGCTCGACCTTTCAGGAAGAAGACTGGGAAGTGTCTTTATACCTGTGCAGTTCGTCCAGCTGCTATCTATGGACCAGGTGAAGAAAGGCACTTTCCAAGGATAGTGTCTCTGGCAAAGTTAGGCCTGATTCCGTTCAAAATTGGCGAACCAAATGTGAAAACAGACTGGATTTATGTGGATAATCTAGTACTTGCGCTGATAATGGCAAGCATGGGACTTTTGGATGACATTCCTGGGAAAAATAAAGAAAGGCATCCCATAGCTGCTGGCCAGCCCTATTTTGTATCTGATGGGTCTCCAGTCAACAGCTTTGAGTTCATCCGACCTTTGCTGAGAAGCTTGAAATATGACCTACCTAAGGCTTCTTTGTCTGTACCTCGTGCGCTTCTTTTGGGAAGGGTTTTCTGGGCAGTCTATACTCTATTATATCCATGGTTGAATCGATGGTGGCTTCCGCAGCCTTTCATCCTTCCTGCTGAAGTTTACAAGGTTGGCGTTACACATTACTTCTCTTTCCTTAAAGCTAAGGAGGAACTTGGATATGTCCCAATGGTGACTCCTCAAGAGGGCATGGCTGCTACAATCTCGTACTGGCAAGAGAGGAAACGGAAAACTCTGGATGGCCCTACAATTTACACATGGCTATTTTGTGTGATCGGAATGGCCACACTGTTTTGTGCTGCTTATTTGCCAGACATAGGGCCAGTGCCACTCTTCAGAGCTATTAGCCTCTTCTTCTTCCGGTCGATGCGAGTATTAAGGATTGTATTCCTTGTAGCTGCAGGATTACACATCGTAGAGGGTGTGTACGCGTGGCACCTGGCCAAAAGGGTGGACCCTGCTAACTCAAGAGGATGGTTTTGGCAGACATTTGTGCTTGGGTTTTTCTCATTACGTTTTCTGTTGAAGAGAGCCCGGAAGGCAGGAGCTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD DUF4499

Protein Analysis

485

Amino Acids

54.68

Weight (kDa)

9.42

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 13 - 130 6.3e-07 NmrA-like family
3Beta_HSD PF01073 15 - 295 1.1e-82 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 15 - 242 2.6e-33 NAD dependent epimerase/dehydratase family
RmlD_sub_bind PF04321 15 - 175 3.4e-09 RmlD substrate binding domain
Polysacc_synt_2 PF02719 15 - 130 6.3e-08 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 16 - 241 1.2e-18 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 18 - 133 8.3e-15 NAD(P)H-binding
NAD_binding_4 PF07993 18 - 236 4.4e-08 Male sterility protein
TMEM254 PF14934 389 - 475 9.4e-30 Transmembrane protein 254
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33630
fragaria_vesca FvH4_5g26130 FvH4_5g26130
malus_domestica MD06G1004300.v1.1
prunus_persica Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1 Prupe.5G005700_v2.0.a1
pyrus_communis pycom06g00410
rosa_chinensis RchiOBHm_Chr7g0216661
rosa_laevigata RLG00000002552
rosa_multiflora Rmu_sc0029435.1_g000001 Rmu_ssc0000117.1_g000009
rosa_roxburghii Rroxscaffold_3G00243030
rosa_rugosa Rorug07G0161500
rosa_samantha Rh7AG301300 Rh7BG292800 Rh7CG319900 Rh7DG301900
rosa_wichuraiana Rw7G025570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
Acc36I ACCTGC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 1335
AccII CGCG 1 cut(s) 1331
AccIII TCCGGA 1 cut(s) 96
AciI CCGC 6 cut(s) 51, 75, 133, 163, 229, 974
AclWI GGATC 1 cut(s) 461
AcoI YGGCCR 4 cut(s) 475, 787, 1182, 1341
AcuI CTGAAG 2 cut(s) 1017, 1219
AcvI CACGTG 1 cut(s) 345
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 5 cut(s) 515, 708, 894, 1109, 1328
AfiI CCNNNNNNNGG 5 cut(s) 147, 208, 877, 1085, 1217
AflIII ACRYGT 3 cut(s) 342, 344, 1329
AgsI TTSAA 6 cut(s) 218, 365, 655, 862, 958, 1426
AjnI CCWGG 3 cut(s) 592, 751, 1338
AleI CACNNNNGTG 1 cut(s) 41
Alw21I GWGCWC 3 cut(s) 109, 386, 1450
Alw26I GTCTC 2 cut(s) 630, 819
AlwI GGATC 1 cut(s) 461
Aor13HI TCCGGA 1 cut(s) 96
AoxI GGCC 8 cut(s) 432, 475, 640, 787, 1141, 1182, 1220, 1341
ApeKI GCWGC 8 cut(s) 91, 286, 578, 782, 976, 1094, 1199, 1298
AspLEI GCGC 3 cut(s) 441, 715, 904
AspS9I GGNCC 5 cut(s) 150, 590, 1142, 1220, 1354
AsuC2I CCSGG 1 cut(s) 1435
AsuHPI GGTGA 3 cut(s) 101, 608, 1084
AvaII GGWCC 3 cut(s) 150, 590, 1354
AxyI CCTNAGG 1 cut(s) 876
BalI TGGCCA 3 cut(s) 789, 1184, 1343
BanI GGYRCC 1 cut(s) 1335
BanII GRGCYC 2 cut(s) 1435, 1450
BauI CACGAG 1 cut(s) 897
BbrPI CACGTG 1 cut(s) 345
BbsI GAAGAC 1 cut(s) 545
Bbv12I GWGCWC 3 cut(s) 109, 386, 1450
BbvI GCAGC 8 cut(s) 78, 273, 565, 769, 988, 1081, 1186, 1285
BccI CCATC 4 cut(s) 803, 957, 1133, 1368
BceAI ACGGC 1 cut(s) 187
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BciT130I CCWGG 3 cut(s) 594, 753, 1340
BcnI CCSGG 1 cut(s) 1435
BcoDI GTCTC 2 cut(s) 630, 819
BfaI CTAG 1 cut(s) 704
BfmI CTRYAG 2 cut(s) 287, 1299
BfuAI ACCTGC 1 cut(s) 335
BglI GCCNNNNNGGC 2 cut(s) 171, 1439
BisI GCNGC 8 cut(s) 92, 287, 579, 783, 977, 1095, 1200, 1299
BlsI GCNGC 8 cut(s) 93, 288, 580, 784, 978, 1096, 1201, 1300
BmcAI AGTACT 1 cut(s) 708
Bme1390I CCNGG 4 cut(s) 594, 753, 1340, 1435
Bme18I GGWCC 3 cut(s) 150, 590, 1354
BmgT120I GGNCC 5 cut(s) 150, 590, 1142, 1220, 1354
BmiI GGNNCC 4 cut(s) 73, 325, 1337, 1356
BmrFI CCNGG 4 cut(s) 594, 753, 1340, 1435
BmrI ACTGGG 1 cut(s) 552
BmsI GCATC 2 cut(s) 781, 1257
BmuI ACTGGG 1 cut(s) 552
BoxI GACNNNNGTC 1 cut(s) 345
BpiI GAAGAC 1 cut(s) 545
BplI GAGNNNNNCTC 2 cut(s) 91, 123
BpmI CTGGAG 1 cut(s) 801
Bpu10I CCTNAGC 2 cut(s) 8, 1044
BpuEI CTTGAG 3 cut(s) 371, 1065, 1351
BpuMI CCSGG 1 cut(s) 1435
Bsa29I ATCGAT 1 cut(s) 961
BsaAI YACGTR 1 cut(s) 345
BsaHI GRCGYC 1 cut(s) 174
BsaI GGTCTC 1 cut(s) 819
BsaJI CCNNGG 5 cut(s) 146, 187, 614, 752, 950
BsaWI WCCGGW 2 cut(s) 96, 1260
BsaXI ACNNNNNCTCC 2 cut(s) 127, 157
Bsc4I CCNNNNNNNGG 5 cut(s) 147, 208, 877, 1085, 1217
Bse1I ACTGG 5 cut(s) 547, 689, 818, 1115, 1223
Bse21I CCTNAGG 1 cut(s) 876
BseAI TCCGGA 1 cut(s) 96
BseBI CCWGG 3 cut(s) 594, 753, 1340
BseCI ATCGAT 1 cut(s) 961
BseDI CCNNGG 5 cut(s) 146, 187, 614, 752, 950
BseGI GGATG 8 cut(s) 199, 217, 748, 772, 837, 984, 1144, 1379
BseLI CCNNNNNNNGG 5 cut(s) 147, 208, 877, 1085, 1217
BseMII CTCAG 1 cut(s) 842
BseNI ACTGG 5 cut(s) 547, 689, 818, 1115, 1223
BseRI GAGGAG 1 cut(s) 1068
BseXI GCAGC 8 cut(s) 78, 273, 565, 769, 988, 1081, 1186, 1285
BsgI GTGCAG 1 cut(s) 585
Bsh1236I CGCG 1 cut(s) 1331
Bsh1285I CGRYCG 3 cut(s) 157, 298, 1265
BshFI GGCC 8 cut(s) 434, 477, 642, 789, 1143, 1184, 1222, 1343
BshNI GGYRCC 1 cut(s) 1335
BshVI ATCGAT 1 cut(s) 961
BsiEI CGRYCG 3 cut(s) 157, 298, 1265
BsiHKAI GWGCWC 3 cut(s) 109, 386, 1450
BsiSI CCGG 4 cut(s) 97, 478, 1261, 1435
BslFI GGGAC 3 cut(s) 420, 747, 1049
BslI CCNNNNNNNGG 5 cut(s) 147, 208, 877, 1085, 1217
BsmAI GTCTC 2 cut(s) 630, 819
BsmFI GGGAC 3 cut(s) 420, 747, 1049
BsnI GGCC 8 cut(s) 434, 477, 642, 789, 1143, 1184, 1222, 1343
Bso31I GGTCTC 1 cut(s) 819
Bsp1286I GDGCHC 4 cut(s) 109, 386, 1435, 1450
Bsp13I TCCGGA 1 cut(s) 96
Bsp143I GATC 4 cut(s) 127, 154, 466, 1173
Bsp19I CCATGG 1 cut(s) 950
BspACI CCGC 6 cut(s) 51, 75, 133, 163, 229, 974
BspANI GGCC 8 cut(s) 434, 477, 642, 789, 1143, 1184, 1222, 1343
BspCNI CTCAG 1 cut(s) 843
BspDI ATCGAT 1 cut(s) 961
BspEI TCCGGA 1 cut(s) 96
BspFNI CGCG 1 cut(s) 1331
BspLI GGNNCC 4 cut(s) 73, 325, 1337, 1356
BspMAI CTGCAG 2 cut(s) 291, 1303
BspMI ACCTGC 1 cut(s) 335
BspPI GGATC 1 cut(s) 461
BspT107I GGYRCC 1 cut(s) 1335
BspTNI GGTCTC 1 cut(s) 819
BsrI ACTGG 5 cut(s) 547, 689, 818, 1115, 1223
BssECI CCNNGG 5 cut(s) 146, 187, 614, 752, 950
BssMI GATC 4 cut(s) 127, 154, 466, 1173
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 897
BssT1I CCWWGG 4 cut(s) 146, 187, 614, 950
Bst2BI CACGAG 1 cut(s) 897
Bst2UI CCWGG 3 cut(s) 594, 753, 1340
Bst4CI ACNGT 2 cut(s) 242, 1191
Bst6I CTCTTC 3 cut(s) 1238, 1256, 1421
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 345
BstC8I GCNNGC 4 cut(s) 167, 727, 787, 791
BstDEI CTNAG 5 cut(s) 8, 337, 851, 876, 1044
BstDSI CCRYGG 1 cut(s) 950
BstENI CCTNNNNNAGG 1 cut(s) 875
BstF5I GGATG 8 cut(s) 199, 217, 748, 772, 837, 984, 1144, 1379
BstFNI CGCG 1 cut(s) 1331
BstHHI GCGC 3 cut(s) 441, 715, 904
BstKTI GATC 4 cut(s) 130, 157, 469, 1176
BstMAI GTCTC 2 cut(s) 630, 819
BstMBI GATC 4 cut(s) 127, 154, 466, 1173
BstMCI CGRYCG 3 cut(s) 157, 298, 1265
BstMWI GCNNNNNNNGC 6 cut(s) 30, 69, 171, 180, 976, 1439
BstNI CCWGG 3 cut(s) 594, 753, 1340
BstNSI RCATGY 1 cut(s) 271
BstPAI GACNNNNGTC 1 cut(s) 345
BstSCI CCNGG 4 cut(s) 592, 751, 1338, 1433
BstSFI CTRYAG 2 cut(s) 287, 1299
BstUI CGCG 1 cut(s) 1331
BstV1I GCAGC 8 cut(s) 78, 273, 565, 769, 988, 1081, 1186, 1285
BstV2I GAAGAC 1 cut(s) 545
BstXI CCANNNNNNTGG 1 cut(s) 1351
Bsu15I ATCGAT 1 cut(s) 961
Bsu36I CCTNAGG 1 cut(s) 876
BsuRI GGCC 8 cut(s) 434, 477, 642, 789, 1143, 1184, 1222, 1343
BsuTUI ATCGAT 1 cut(s) 961
BtgI CCRYGG 1 cut(s) 950
BtsCI GGATG 8 cut(s) 199, 217, 748, 772, 837, 984, 1144, 1379
BtsI GCAGTG 1 cut(s) 178
BtsIMutI CAGTG 3 cut(s) 178, 1187, 1230
BveI ACCTGC 1 cut(s) 335
Cac8I GCNNGC 4 cut(s) 167, 727, 787, 791
CfoI GCGC 3 cut(s) 441, 715, 904
Cfr13I GGNCC 5 cut(s) 150, 590, 1142, 1220, 1354
ClaI ATCGAT 1 cut(s) 961
CseI GACGC 2 cut(s) 163, 172
CsiI ACCWGGT 1 cut(s) 592
Csp6I GTAC 5 cut(s) 514, 707, 893, 1108, 1327
CviAII CATG 6 cut(s) 268, 461, 730, 951, 1090, 1158
CviQI GTAC 5 cut(s) 514, 707, 893, 1108, 1327
DdeI CTNAG 5 cut(s) 8, 337, 851, 876, 1044
DpnI GATC 4 cut(s) 129, 156, 468, 1175
DpnII GATC 4 cut(s) 127, 154, 466, 1173
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 4 cut(s) 475, 787, 1182, 1341
Eam1104I CTCTTC 3 cut(s) 1238, 1256, 1421
EarI CTCTTC 3 cut(s) 1238, 1256, 1421
EciI GGCGGA 1 cut(s) 152
Ecl136II GAGCTC 1 cut(s) 1448
Eco130I CCWWGG 4 cut(s) 146, 187, 614, 950
Eco147I AGGCCT 2 cut(s) 434, 642
Eco24I GRGCYC 2 cut(s) 1435, 1450
Eco31I GGTCTC 1 cut(s) 819
Eco47I GGWCC 3 cut(s) 150, 590, 1354
Eco53kI GAGCTC 1 cut(s) 1448
Eco57I CTGAAG 2 cut(s) 1017, 1219
Eco72I CACGTG 1 cut(s) 345
Eco81I CCTNAGG 1 cut(s) 876
EcoICRI GAGCTC 1 cut(s) 1448
EcoNI CCTNNNNNAGG 1 cut(s) 875
EcoRII CCWGG 3 cut(s) 592, 751, 1338
EcoT14I CCWWGG 4 cut(s) 146, 187, 614, 950
EcoT38I GRGCYC 2 cut(s) 1435, 1450
ErhI CCWWGG 4 cut(s) 146, 187, 614, 950
FaeI CATG 6 cut(s) 271, 464, 733, 954, 1093, 1161
FaqI GGGAC 3 cut(s) 420, 747, 1049
FatI CATG 6 cut(s) 267, 460, 729, 950, 1089, 1157
FauI CCCGC 1 cut(s) 222
FauNDI CATATG 1 cut(s) 472
Fnu4HI GCNGC 8 cut(s) 92, 287, 579, 783, 977, 1095, 1200, 1299
FokI GGATG 8 cut(s) 206, 224, 755, 759, 824, 971, 1151, 1386
FriOI GRGCYC 2 cut(s) 1435, 1450
Fsp4HI GCNGC 8 cut(s) 92, 287, 579, 783, 977, 1095, 1200, 1299
FspBI CTAG 1 cut(s) 704
GlaI GCGC 3 cut(s) 440, 714, 903
GluI GCNGC 8 cut(s) 92, 287, 579, 783, 977, 1095, 1200, 1299
GsuI CTGGAG 1 cut(s) 801
HaeIII GGCC 8 cut(s) 434, 477, 642, 789, 1143, 1184, 1222, 1343
HapII CCGG 4 cut(s) 97, 478, 1261, 1435
HgaI GACGC 2 cut(s) 163, 172
HhaI GCGC 3 cut(s) 441, 715, 904
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 6 cut(s) 271, 464, 733, 954, 1093, 1161
Hin6I GCGC 3 cut(s) 439, 713, 902
HinP1I GCGC 3 cut(s) 439, 713, 902
HincII GTYRAC 2 cut(s) 313, 823
HindII GTYRAC 2 cut(s) 313, 823
HindIII AAGCTT 1 cut(s) 856
HinfI GANTC 4 cut(s) 361, 646, 958, 1075
HpaII CCGG 4 cut(s) 97, 478, 1261, 1435
HphI GGTGA 3 cut(s) 101, 608, 1084
Hpy166II GTNNAC 6 cut(s) 178, 313, 823, 1003, 1327, 1354
Hpy188I TCNGA 6 cut(s) 119, 154, 808, 842, 1178, 1238
Hpy188III TCNNGA 8 cut(s) 97, 273, 350, 358, 532, 1082, 1136, 1368
Hpy8I GTNNAC 6 cut(s) 178, 313, 823, 1003, 1327, 1354
HpyAV CCTTC 4 cut(s) 14, 909, 998, 1432
HpyCH4III ACNGT 2 cut(s) 242, 1191
HpyCH4IV ACGT 2 cut(s) 344, 1414
HpyCH4V TGCA 4 cut(s) 4, 289, 566, 1301
HpyF10VI GCNNNNNNNGC 6 cut(s) 30, 69, 171, 180, 976, 1439
HpyF3I CTNAG 5 cut(s) 8, 337, 851, 876, 1044
HpySE526I ACGT 2 cut(s) 344, 1414
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 6 cut(s) 271, 464, 733, 954, 1093, 1161
HspAI GCGC 3 cut(s) 439, 713, 902
Kpn2I TCCGGA 1 cut(s) 96
Kzo9I GATC 4 cut(s) 127, 154, 466, 1173
LmnI GCTCC 6 cut(s) 77, 88, 99, 233, 1445, 1453
Lsp1109I GCAGC 8 cut(s) 78, 273, 565, 769, 988, 1081, 1186, 1285
LweI GCATC 2 cut(s) 781, 1257
MabI ACCWGGT 1 cut(s) 592
MaeI CTAG 1 cut(s) 704
MaeII ACGT 2 cut(s) 344, 1414
MaeIII GTNAC 3 cut(s) 196, 1015, 1072
MalI GATC 4 cut(s) 129, 156, 468, 1175
MboI GATC 4 cut(s) 127, 154, 466, 1173
MboII GAAGA 8 cut(s) 547, 550, 611, 1225, 1243, 1246, 1249, 1438
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 4 cut(s) 109, 386, 1435, 1450
MlsI TGGCCA 3 cut(s) 789, 1184, 1343
MluCI AATT 3 cut(s) 489, 657, 1149
MluI ACGCGT 1 cut(s) 1329
MluNI TGGCCA 3 cut(s) 789, 1184, 1343
MlyI GAGTC 1 cut(s) 1069
MmeI TCCRAC 1 cut(s) 865
Mox20I TGGCCA 3 cut(s) 789, 1184, 1343
MroI TCCGGA 1 cut(s) 96
MscI TGGCCA 3 cut(s) 789, 1184, 1343
MseI TTAA 4 cut(s) 509, 1038, 1277, 1453
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 3 cut(s) 789, 1184, 1343
MspA1I CMGCKG 2 cut(s) 51, 578
MspI CCGG 4 cut(s) 97, 478, 1261, 1435
MspR9I CCNGG 4 cut(s) 594, 753, 1340, 1435
MunI CAATTG 1 cut(s) 489
MvaI CCWGG 3 cut(s) 594, 753, 1340
MvnI CGCG 1 cut(s) 1331
MwoI GCNNNNNNNGC 6 cut(s) 30, 69, 171, 180, 976, 1439
NciI CCSGG 1 cut(s) 1435
NcoI CCATGG 1 cut(s) 950
NdeI CATATG 1 cut(s) 472
NdeII GATC 4 cut(s) 127, 154, 466, 1173
NlaIII CATG 6 cut(s) 271, 464, 733, 954, 1093, 1161
NlaIV GGNNCC 4 cut(s) 73, 325, 1337, 1356
NmeAIII GCCGAG 1 cut(s) 520
NmuCI GTSAC 1 cut(s) 1072
NspI RCATGY 1 cut(s) 271
OliI CACNNNNGTG 1 cut(s) 41
PceI AGGCCT 2 cut(s) 434, 642
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 3 cut(s) 361, 646, 958
PkrI GCNGC 8 cut(s) 93, 288, 580, 784, 978, 1096, 1201, 1300
Ple19I CGATCG 1 cut(s) 157
PleI GAGTC 1 cut(s) 1069
PmaCI CACGTG 1 cut(s) 345
PmlI CACGTG 1 cut(s) 345
PpsI GAGTC 1 cut(s) 1069
Ppu21I YACGTR 1 cut(s) 345
PshAI GACNNNNGTC 1 cut(s) 345
Psp124BI GAGCTC 1 cut(s) 1450
Psp6I CCWGG 3 cut(s) 592, 751, 1338
PspCI CACGTG 1 cut(s) 345
PspGI CCWGG 3 cut(s) 592, 751, 1338
PspN4I GGNNCC 4 cut(s) 73, 325, 1337, 1356
PspPI GGNCC 5 cut(s) 150, 590, 1142, 1220, 1354
PstI CTGCAG 2 cut(s) 291, 1303
PvuI CGATCG 1 cut(s) 157
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 5 cut(s) 515, 708, 894, 1109, 1328
RsaNI GTAC 5 cut(s) 514, 707, 893, 1108, 1327
RseI CAYNNNNRTG 1 cut(s) 41
SacI GAGCTC 1 cut(s) 1450
SaqAI TTAA 4 cut(s) 509, 1038, 1277, 1453
SatI GCNGC 8 cut(s) 92, 287, 579, 783, 977, 1095, 1200, 1299
Sau3AI GATC 4 cut(s) 127, 154, 466, 1173
Sau96I GGNCC 5 cut(s) 150, 590, 1142, 1220, 1354
ScaI AGTACT 1 cut(s) 708
SchI GAGTC 1 cut(s) 1069
ScrFI CCNGG 4 cut(s) 594, 753, 1340, 1435
SduI GDGCHC 4 cut(s) 109, 386, 1435, 1450
SexAI ACCWGGT 1 cut(s) 592
SfaNI GCATC 2 cut(s) 781, 1257
SfcI CTRYAG 2 cut(s) 287, 1299
SinI GGWCC 3 cut(s) 150, 590, 1354
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 3 cut(s) 350, 1080, 1366
SmoI CTYRAG 3 cut(s) 350, 1080, 1366
Sse9I AATT 3 cut(s) 489, 657, 1149
SseBI AGGCCT 2 cut(s) 434, 642
SsiI CCGC 6 cut(s) 51, 75, 133, 163, 229, 974
SspMI CTAG 1 cut(s) 704
SstI GAGCTC 1 cut(s) 1450
StuI AGGCCT 2 cut(s) 434, 642
StyD4I CCNGG 4 cut(s) 592, 751, 1338, 1433
StyI CCWWGG 4 cut(s) 146, 187, 614, 950
TaaI ACNGT 2 cut(s) 242, 1191
TaiI ACGT 2 cut(s) 347, 1417
TaqI TCGA 6 cut(s) 303, 453, 465, 523, 961, 1265
TaqII GACCGA 1 cut(s) 284
TasI AATT 3 cut(s) 489, 657, 1149
TatI WGTACW 2 cut(s) 513, 706
TfiI GAWTC 3 cut(s) 361, 646, 958
Tru1I TTAA 4 cut(s) 509, 1038, 1277, 1453
Tru9I TTAA 4 cut(s) 509, 1038, 1277, 1453
TscAI CASTG 3 cut(s) 185, 1194, 1230
TseFI GTSAC 1 cut(s) 1072
TseI GCWGC 8 cut(s) 91, 286, 578, 782, 976, 1094, 1199, 1298
Tsp45I GTSAC 1 cut(s) 1072
TspDTI ATGAA 2 cut(s) 826, 973
TspGWI ACGGA 2 cut(s) 639, 1141
TspRI CASTG 3 cut(s) 185, 1194, 1230
VpaK11BI GGWCC 3 cut(s) 150, 590, 1354
XagI CCTNNNNNAGG 1 cut(s) 875
XceI RCATGY 1 cut(s) 271
XspI CTAG 1 cut(s) 704
ZrmI AGTACT 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.