Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003104

Physical Location & Seq

Genomic Coordinates Forward (+)

14288323 .. 14289313

Gene Structure 991 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G33775.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 333 bp

ATGGGTATCAAGATCTTGTTGATCCTTGGCCTCTTGACCCTGGCTGTAGTCGCCGAGTCAGCCAATGCCACATGGACCCTTACCAAATCTTGTGTCAATGGCCAAGGTTGCATTGGAGAGGATGGTGAGCTTGACTACTTGATGGATTCTGAGACCAACCGGCGCCAACTAGCCGCGAGGCGCAGCTACATCAGTTACGGTGCTCTCAGGAAGAACAATGTGCCGTGCAGCCGACGTGGCCGGTCTTACTACGATTGCAAGAAGAGGAAAAGGGCTAACCCTTACAGACGTGGCTGCAGCGTCATCACGCATTGCTACAGGCAAACTTCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005623 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0010769 GO:0019722 GO:0019932 GO:0022603 GO:0022604 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0040008 GO:0042995 GO:0043900 GO:0044464 GO:0045595 GO:0048018 GO:0048046 GO:0048583 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0055044 GO:0060284 GO:0065007 GO:0065009 GO:0080092 GO:0090406 GO:0098772 GO:0120025 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

110

Amino Acids

12.4

Weight (kDa)

9.77

Isoelectric Point (pI)

53.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	46 - 107	2.3e-27	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28270 AT2G33775 AT3G05490
fragaria_vesca	FvH4_3g09010 FvH4_3g09020
malus_domestica	MD00G1116200.v1.1 MD10G1292800.v1.1 MD10G1292900.v1.1
prunus_persica	Prupe.4G049000_v2.0.a1
pyrus_communis	pycom05g28940 pycom10g24490
rosa_chinensis	RchiOBHm_Chr5g0008651 RchiOBHm_Chr5g0008661 RchiOBHm_Chr5g0008671
rosa_laevigata	RLG00000031590 RLG00000031591 RLG00000031592
rosa_multiflora	Rmu_sc0001665.1_g000030 Rmu_sc0001665.1_g000031 Rmu_sc0001665.1_g000040
rosa_roxburghii	Rroxscaffold_1G00067890 Rroxscaffold_1G00067900 Rroxscaffold_1G00067910
rosa_rugosa	Rorug04G0437700 Rorug04G0437700 Rorug04G0437700
rosa_samantha	Rh5AG066300 Rh5BG063600 Rh5BG063700 Rh5CG073800 Rh5CG073900 Rh5CG074100 Rh5DG062500 Rh5DG062600
rosa_wichuraiana	Rw5G006230 Rw5G006260 Rw5G006270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	162
AccII	CGCG	1 cut(s)	176
AciI	CCGC	1 cut(s)	174
AclWI	GGATC	1 cut(s)	16
AcoI	YGGCCR	2 cut(s)	100, 238
AcyI	GRCGYC	1 cut(s)	163
AjiI	CACGTC	2 cut(s)	236, 290
AjnI	CCWGG	1 cut(s)	39
AluBI	AGCT	2 cut(s)	130, 186
AluI	AGCT	2 cut(s)	130, 186
Alw21I	GWGCWC	1 cut(s)	205
Alw26I	GTCTC	1 cut(s)	146
AlwI	GGATC	1 cut(s)	16
AoxI	GGCC	3 cut(s)	28, 100, 238
ApeKI	GCWGC	4 cut(s)	183, 228, 294, 297
ArsI	GACNNNNNNTTYG	2 cut(s)	78, 110
AspLEI	GCGC	2 cut(s)	165, 183
AspS9I	GGNCC	1 cut(s)	75
AsuHPI	GGTGA	1 cut(s)	137
AvaII	GGWCC	1 cut(s)	75
BalI	TGGCCA	1 cut(s)	102
BanI	GGYRCC	1 cut(s)	162
Bbv12I	GWGCWC	1 cut(s)	205
BbvI	GCAGC	4 cut(s)	195, 240, 281, 309
BccI	CCATC	2 cut(s)	116, 136
BceAI	ACGGC	1 cut(s)	208
BciT130I	CCWGG	1 cut(s)	41
BcoDI	GTCTC	1 cut(s)	146
BfaI	CTAG	1 cut(s)	170
BfmI	CTRYAG	3 cut(s)	45, 295, 316
BfoI	RGCGCY	1 cut(s)	166
BglI	GCCNNNNNGGC	1 cut(s)	237
BglII	AGATCT	1 cut(s)	12
BisI	GCNGC	5 cut(s)	174, 184, 229, 295, 298
BlsI	GCNGC	5 cut(s)	175, 185, 230, 296, 299
Bme1390I	CCNGG	1 cut(s)	41
Bme18I	GGWCC	1 cut(s)	75
BmgBI	CACGTC	2 cut(s)	236, 290
BmgT120I	GGNCC	1 cut(s)	75
BmiI	GGNNCC	2 cut(s)	77, 164
BmrFI	CCNGG	1 cut(s)	41
BsaHI	GRCGYC	1 cut(s)	163
BsaI	GGTCTC	1 cut(s)	146
BsaJI	CCNNGG	3 cut(s)	25, 39, 103
Bse118I	RCCGGY	2 cut(s)	159, 240
Bse3DI	GCAATG	1 cut(s)	310
BseBI	CCWGG	1 cut(s)	41
BseDI	CCNNGG	3 cut(s)	25, 39, 103
BseGI	GGATG	1 cut(s)	127
BseMI	GCAATG	1 cut(s)	310
BseMII	CTCAG	2 cut(s)	141, 220
BseXI	GCAGC	4 cut(s)	195, 240, 281, 309
BsgI	GTGCAG	1 cut(s)	247
Bsh1236I	CGCG	1 cut(s)	176
BshFI	GGCC	3 cut(s)	30, 102, 240
BshNI	GGYRCC	1 cut(s)	162
BsiHKAI	GWGCWC	1 cut(s)	205
BsiSI	CCGG	2 cut(s)	160, 241
BsmAI	GTCTC	1 cut(s)	146
BsnI	GGCC	3 cut(s)	30, 102, 240
Bso31I	GGTCTC	1 cut(s)	146
Bsp1286I	GDGCHC	1 cut(s)	205
Bsp143I	GATC	2 cut(s)	12, 21
BspACI	CCGC	1 cut(s)	174
BspANI	GGCC	3 cut(s)	30, 102, 240
BspCNI	CTCAG	2 cut(s)	142, 219
BspFNI	CGCG	1 cut(s)	176
BspLI	GGNNCC	2 cut(s)	77, 164
BspMAI	CTGCAG	1 cut(s)	299
BspPI	GGATC	1 cut(s)	16
BspT107I	GGYRCC	1 cut(s)	162
BspTNI	GGTCTC	1 cut(s)	146
BsrDI	GCAATG	1 cut(s)	310
BsrFI	RCCGGY	2 cut(s)	159, 240
BssAI	RCCGGY	2 cut(s)	159, 240
BssECI	CCNNGG	3 cut(s)	25, 39, 103
BssMI	GATC	2 cut(s)	12, 21
BssNI	GRCGYC	1 cut(s)	163
BssT1I	CCWWGG	2 cut(s)	25, 103
Bst2UI	CCWGG	1 cut(s)	41
Bst4CI	ACNGT	1 cut(s)	200
Bst6I	CTCTTC	1 cut(s)	257
BstACI	GRCGYC	1 cut(s)	163
BstDEI	CTNAG	2 cut(s)	150, 206
BstF5I	GGATG	1 cut(s)	127
BstFNI	CGCG	1 cut(s)	176
BstH2I	RGCGCY	1 cut(s)	166
BstHHI	GCGC	2 cut(s)	165, 183
BstKTI	GATC	2 cut(s)	15, 24
BstMAI	GTCTC	1 cut(s)	146
BstMBI	GATC	2 cut(s)	12, 21
BstMWI	GCNNNNNNNGC	4 cut(s)	50, 59, 108, 237
BstNI	CCWGG	1 cut(s)	41
BstSCI	CCNGG	1 cut(s)	39
BstSFI	CTRYAG	3 cut(s)	45, 295, 316
BstUI	CGCG	1 cut(s)	176
BstV1I	GCAGC	4 cut(s)	195, 240, 281, 309
BstX2I	RGATCY	1 cut(s)	12
BstYI	RGATCY	1 cut(s)	12
BsuRI	GGCC	3 cut(s)	30, 102, 240
BtrI	CACGTC	2 cut(s)	236, 290
BtsCI	GGATG	1 cut(s)	127
CfoI	GCGC	2 cut(s)	165, 183
Cfr10I	RCCGGY	2 cut(s)	159, 240
Cfr13I	GGNCC	1 cut(s)	75
CseI	GACGC	1 cut(s)	289
CviAII	CATG	1 cut(s)	72
DdeI	CTNAG	2 cut(s)	150, 206
DinI	GGCGCC	1 cut(s)	164
DpnI	GATC	2 cut(s)	14, 23
DpnII	GATC	2 cut(s)	12, 21
EaeI	YGGCCR	2 cut(s)	100, 238
Eam1104I	CTCTTC	1 cut(s)	257
EarI	CTCTTC	1 cut(s)	257
Eco130I	CCWWGG	2 cut(s)	25, 103
Eco31I	GGTCTC	1 cut(s)	146
Eco47I	GGWCC	1 cut(s)	75
EcoRII	CCWGG	1 cut(s)	39
EcoT14I	CCWWGG	2 cut(s)	25, 103
EgeI	GGCGCC	1 cut(s)	164
EheI	GGCGCC	1 cut(s)	164
ErhI	CCWWGG	2 cut(s)	25, 103
FaeI	CATG	1 cut(s)	75
FaiI	YATR	1 cut(s)	73
FatI	CATG	1 cut(s)	71
Fnu4HI	GCNGC	5 cut(s)	174, 184, 229, 295, 298
FokI	GGATG	1 cut(s)	134
Fsp4HI	GCNGC	5 cut(s)	174, 184, 229, 295, 298
FspBI	CTAG	1 cut(s)	170
GlaI	GCGC	2 cut(s)	164, 182
GluI	GCNGC	5 cut(s)	174, 184, 229, 295, 298
HaeII	RGCGCY	1 cut(s)	166
HaeIII	GGCC	3 cut(s)	30, 102, 240
HapII	CCGG	2 cut(s)	160, 241
HgaI	GACGC	1 cut(s)	289
HhaI	GCGC	2 cut(s)	165, 183
Hin1I	GRCGYC	1 cut(s)	163
Hin1II	CATG	1 cut(s)	75
Hin6I	GCGC	2 cut(s)	163, 181
HinP1I	GCGC	2 cut(s)	163, 181
HinfI	GANTC	2 cut(s)	56, 146
HpaII	CCGG	2 cut(s)	160, 241
HphI	GGTGA	1 cut(s)	137
Hpy188I	TCNGA	1 cut(s)	151
Hpy188III	TCNNGA	3 cut(s)	10, 34, 208
Hpy99I	CGWCG	1 cut(s)	237
HpyCH4III	ACNGT	1 cut(s)	200
HpyCH4IV	ACGT	2 cut(s)	235, 289
HpyCH4V	TGCA	4 cut(s)	111, 228, 258, 297
HpyF10VI	GCNNNNNNNGC	4 cut(s)	50, 59, 108, 237
HpyF3I	CTNAG	2 cut(s)	150, 206
HpySE526I	ACGT	2 cut(s)	235, 289
Hsp92I	GRCGYC	1 cut(s)	163
Hsp92II	CATG	1 cut(s)	75
HspAI	GCGC	2 cut(s)	163, 181
KasI	GGCGCC	1 cut(s)	162
Kzo9I	GATC	2 cut(s)	12, 21
LpnPI	CCDG	6 cut(s)	26, 53, 173, 193, 254, 304
Lsp1109I	GCAGC	4 cut(s)	195, 240, 281, 309
MaeI	CTAG	1 cut(s)	170
MaeII	ACGT	2 cut(s)	235, 289
MaeIII	GTNAC	1 cut(s)	194
MalI	GATC	2 cut(s)	14, 23
MboI	GATC	2 cut(s)	12, 21
MboII	GAAGA	2 cut(s)	223, 274
MflI	RGATCY	1 cut(s)	12
MhlI	GDGCHC	1 cut(s)	205
MlsI	TGGCCA	1 cut(s)	102
MluNI	TGGCCA	1 cut(s)	102
Mly113I	GGCGCC	1 cut(s)	163
MlyI	GAGTC	1 cut(s)	65
MnlI	CCTC	4 cut(s)	41, 112, 171, 258
Mox20I	TGGCCA	1 cut(s)	102
MscI	TGGCCA	1 cut(s)	102
MseI	TTAA	1 cut(s)	331
Msp20I	TGGCCA	1 cut(s)	102
MspI	CCGG	2 cut(s)	160, 241
MspR9I	CCNGG	1 cut(s)	41
MvaI	CCWGG	1 cut(s)	41
MvnI	CGCG	1 cut(s)	176
MwoI	GCNNNNNNNGC	4 cut(s)	50, 59, 108, 237
NarI	GGCGCC	1 cut(s)	163
NdeII	GATC	2 cut(s)	12, 21
NlaIII	CATG	1 cut(s)	75
NlaIV	GGNNCC	2 cut(s)	77, 164
NmeAIII	GCCGAG	1 cut(s)	79
PfeI	GAWTC	1 cut(s)	146
PkrI	GCNGC	5 cut(s)	175, 185, 230, 296, 299
PleI	GAGTC	1 cut(s)	64
PluTI	GGCGCC	1 cut(s)	166
PpsI	GAGTC	1 cut(s)	64
Psp6I	CCWGG	1 cut(s)	39
PspGI	CCWGG	1 cut(s)	39
PspN4I	GGNNCC	2 cut(s)	77, 164
PspPI	GGNCC	1 cut(s)	75
PstI	CTGCAG	1 cut(s)	299
PsuI	RGATCY	1 cut(s)	12
SaqAI	TTAA	1 cut(s)	331
SatI	GCNGC	5 cut(s)	174, 184, 229, 295, 298
Sau3AI	GATC	2 cut(s)	12, 21
Sau96I	GGNCC	1 cut(s)	75
SchI	GAGTC	1 cut(s)	65
ScrFI	CCNGG	1 cut(s)	41
SduI	GDGCHC	1 cut(s)	205
SetI	ASST	5 cut(s)	109, 132, 188, 238, 292
SfcI	CTRYAG	3 cut(s)	45, 295, 316
SfoI	GGCGCC	1 cut(s)	164
SinI	GGWCC	1 cut(s)	75
SsiI	CCGC	1 cut(s)	174
SspDI	GGCGCC	1 cut(s)	162
SspMI	CTAG	1 cut(s)	170
StyD4I	CCNGG	1 cut(s)	39
StyI	CCWWGG	2 cut(s)	25, 103
TaaI	ACNGT	1 cut(s)	200
TaiI	ACGT	2 cut(s)	238, 292
TauI	GCSGC	1 cut(s)	176
TfiI	GAWTC	1 cut(s)	146
Tru1I	TTAA	1 cut(s)	331
Tru9I	TTAA	1 cut(s)	331
TseI	GCWGC	4 cut(s)	183, 228, 294, 297
VpaK11BI	GGWCC	1 cut(s)	75
XcmI	CCANNNNNNNNNTGG	1 cut(s)	110
XspI	CTAG	1 cut(s)	170

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.