FvH4_3g09010

ralf-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003104
Physical Location & Seq
Forward (+)
5266873 .. 5268430
1558 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g09010.t1

Sequence Viewer

Length: 354 bp
ATGGATTTCAAATCCTGCCTTGTTGTGCTCCTCTTGGCCCTAGCCATGGTGGCTGCAGTAGAGTCGTCAACGATATCGATGCACGACGCCGCCTGGGGTCTCAAACACAGCCGCGACGACGTGGGTGACATGATCGGACAAGATAATGAGATGTTACTGGACTCGGAGGTCGGCCGGCGTCAACTGATGGCAAATAATGTCCGTTATATCAGCTACGGTGCCCTTAAGAAAAACAAGGTACCGTGTGGTCAGCCCGGAGCATCTTACTACAACTGCAACAGGCAGAACTCACAGCAGGCCAATGTTTACCGCCGTGGTTGCTCAGTCGCCACCCGTTGCGCCCGCTCCAACTGA

Protein Analysis

118

Amino Acids

12.91

Weight (kDa)

8.91

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 50 - 115 7.9e-24 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 167
Acc65I GGTACC 1 cut(s) 238
AccB1I GGYRCC 2 cut(s) 218, 238
AccBSI CCGCTC 1 cut(s) 345
AccII CGCG 1 cut(s) 114
AciI CCGC 4 cut(s) 90, 112, 310, 343
AcoI YGGCCR 1 cut(s) 172
AcyI GRCGYC 2 cut(s) 87, 178
AfaI GTAC 1 cut(s) 240
AfiI CCNNNNNNNGG 1 cut(s) 46
AflII CTTAAG 1 cut(s) 224
AgsI TTSAA 1 cut(s) 10
AjiI CACGTC 1 cut(s) 121
AjnI CCWGG 1 cut(s) 92
AluBI AGCT 1 cut(s) 213
AluI AGCT 1 cut(s) 213
Alw21I GWGCWC 1 cut(s) 30
Alw26I GTCTC 1 cut(s) 104
AoxI GGCC 3 cut(s) 36, 172, 297
ApeKI GCWGC 1 cut(s) 53
Asp718I GGTACC 1 cut(s) 238
AspLEI GCGC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 37
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 1 cut(s) 137
BaeGI GKGCMC 1 cut(s) 223
BanI GGYRCC 2 cut(s) 218, 238
Bbv12I GWGCWC 1 cut(s) 30
BbvI GCAGC 1 cut(s) 40
BccI CCATC 1 cut(s) 181
BceAI ACGGC 1 cut(s) 297
BcgI CGANNNNNNTGC 4 cut(s) 45, 61, 79, 95
BciT130I CCWGG 1 cut(s) 94
BcnI CCSGG 1 cut(s) 255
BcoDI GTCTC 1 cut(s) 104
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 54
BfrI CTTAAG 1 cut(s) 224
BglI GCCNNNNNGGC 1 cut(s) 50
BisI GCNGC 3 cut(s) 54, 90, 112
BlsI GCNGC 3 cut(s) 55, 91, 113
Bme1390I CCNGG 2 cut(s) 94, 255
BmgBI CACGTC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 2 cut(s) 220, 240
BmrFI CCNGG 2 cut(s) 94, 255
BmsI GCATC 2 cut(s) 69, 269
BpuMI CCSGG 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 77
BsaHI GRCGYC 2 cut(s) 87, 178
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 3 cut(s) 45, 93, 313
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse118I RCCGGY 1 cut(s) 174
Bse1I ACTGG 1 cut(s) 162
BseBI CCWGG 1 cut(s) 94
BseCI ATCGAT 1 cut(s) 77
BseDI CCNNGG 3 cut(s) 45, 93, 313
BseLI CCNNNNNNNGG 1 cut(s) 46
BseMII CTCAG 1 cut(s) 336
BseNI ACTGG 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 223
BseX3I CGGCCG 1 cut(s) 172
BseXI GCAGC 1 cut(s) 40
Bsh1236I CGCG 1 cut(s) 114
Bsh1285I CGRYCG 1 cut(s) 175
BshFI GGCC 3 cut(s) 38, 174, 299
BshNI GGYRCC 2 cut(s) 218, 238
BshVI ATCGAT 1 cut(s) 77
BsiEI CGRYCG 1 cut(s) 175
BsiHKAI GWGCWC 1 cut(s) 30
BsiSI CCGG 2 cut(s) 175, 255
BslI CCNNNNNNNGG 1 cut(s) 46
BsmAI GTCTC 1 cut(s) 104
BsnI GGCC 3 cut(s) 38, 174, 299
Bso31I GGTCTC 1 cut(s) 104
Bsp1286I GDGCHC 2 cut(s) 30, 223
Bsp143I GATC 1 cut(s) 132
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 4 cut(s) 90, 112, 310, 343
BspANI GGCC 3 cut(s) 38, 174, 299
BspCNI CTCAG 1 cut(s) 335
BspDI ATCGAT 1 cut(s) 77
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 2 cut(s) 220, 240
BspMAI CTGCAG 1 cut(s) 58
BspT107I GGYRCC 2 cut(s) 218, 238
BspTI CTTAAG 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 104
BsrBI CCGCTC 1 cut(s) 345
BsrFI RCCGGY 1 cut(s) 174
BsrI ACTGG 1 cut(s) 162
BssAI RCCGGY 1 cut(s) 174
BssECI CCNNGG 3 cut(s) 45, 93, 313
BssMI GATC 1 cut(s) 132
BssNI GRCGYC 2 cut(s) 87, 178
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 94
Bst4CI ACNGT 2 cut(s) 218, 243
BstACI GRCGYC 2 cut(s) 87, 178
BstAFI CTTAAG 1 cut(s) 224
BstC8I GCNNGC 3 cut(s) 176, 297, 343
BstDEI CTNAG 1 cut(s) 322
BstDSI CCRYGG 2 cut(s) 45, 313
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 341
BstKTI GATC 1 cut(s) 135
BstMAI GTCTC 1 cut(s) 104
BstMBI GATC 1 cut(s) 132
BstMCI CGRYCG 1 cut(s) 175
BstMWI GCNNNNNNNGC 2 cut(s) 50, 318
BstNI CCWGG 1 cut(s) 94
BstSCI CCNGG 2 cut(s) 92, 253
BstSFI CTRYAG 1 cut(s) 54
BstSLI GKGCMC 1 cut(s) 223
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 1 cut(s) 40
BstZI CGGCCG 1 cut(s) 172
Bsu15I ATCGAT 1 cut(s) 77
BsuRI GGCC 3 cut(s) 38, 174, 299
BsuTUI ATCGAT 1 cut(s) 77
BtgI CCRYGG 2 cut(s) 45, 313
BtrI CACGTC 1 cut(s) 121
Cac8I GCNNGC 3 cut(s) 176, 297, 343
CfoI GCGC 1 cut(s) 341
Cfr10I RCCGGY 1 cut(s) 174
Cfr13I GGNCC 1 cut(s) 37
ClaI ATCGAT 1 cut(s) 77
CseI GACGC 2 cut(s) 95, 167
Csp6I GTAC 1 cut(s) 239
CviAII CATG 2 cut(s) 46, 130
CviJI RGCY 8 cut(s) 38, 44, 53, 111, 174, 213, 253, 299
CviKI_1 RGCY 8 cut(s) 38, 44, 53, 111, 174, 213, 253, 299
CviQI GTAC 1 cut(s) 239
DdeI CTNAG 1 cut(s) 322
DpnI GATC 1 cut(s) 134
DpnII GATC 1 cut(s) 132
DrdI GACNNNNNNGTC 1 cut(s) 167
DseDI GACNNNNNNGTC 1 cut(s) 167
EaeI YGGCCR 1 cut(s) 172
EagI CGGCCG 1 cut(s) 172
EclXI CGGCCG 1 cut(s) 172
Eco130I CCWWGG 1 cut(s) 45
Eco31I GGTCTC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 75
Eco52I CGGCCG 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 92
EcoRV GATATC 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 2 cut(s) 49, 133
FaiI YATR 3 cut(s) 47, 131, 207
FatI CATG 2 cut(s) 45, 129
FauI CCCGC 1 cut(s) 350
Fnu4HI GCNGC 3 cut(s) 54, 90, 112
Fsp4HI GCNGC 3 cut(s) 54, 90, 112
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 340
GluI GCNGC 3 cut(s) 54, 90, 112
HaeIII GGCC 3 cut(s) 38, 174, 299
HapII CCGG 2 cut(s) 175, 255
HgaI GACGC 2 cut(s) 95, 167
HhaI GCGC 1 cut(s) 341
Hin1I GRCGYC 2 cut(s) 87, 178
Hin1II CATG 2 cut(s) 49, 133
Hin6I GCGC 1 cut(s) 339
HinP1I GCGC 1 cut(s) 339
HincII GTYRAC 2 cut(s) 69, 182
HindII GTYRAC 2 cut(s) 69, 182
HinfI GANTC 2 cut(s) 62, 161
HpaII CCGG 2 cut(s) 175, 255
HphI GGTGA 1 cut(s) 137
Hpy166II GTNNAC 3 cut(s) 69, 182, 307
Hpy188I TCNGA 2 cut(s) 137, 166
Hpy8I GTNNAC 3 cut(s) 69, 182, 307
Hpy99I CGWCG 3 cut(s) 89, 119, 122
HpyCH4III ACNGT 2 cut(s) 218, 243
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 3 cut(s) 56, 82, 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 50, 318
HpyF3I CTNAG 1 cut(s) 322
HpySE526I ACGT 1 cut(s) 120
Hsp92I GRCGYC 2 cut(s) 87, 178
Hsp92II CATG 2 cut(s) 49, 133
HspAI GCGC 1 cut(s) 339
KpnI GGTACC 1 cut(s) 242
KroI GCCGGC 1 cut(s) 174
KroNI GCCGGC 1 cut(s) 176
Kzo9I GATC 1 cut(s) 132
LmnI GCTCC 3 cut(s) 33, 257, 350
LpnPI CCDG 8 cut(s) 28, 79, 106, 143, 188, 265, 268, 281
Lsp1109I GCAGC 1 cut(s) 40
LweI GCATC 2 cut(s) 69, 269
MaeI CTAG 1 cut(s) 41
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 2 cut(s) 125, 153
MalI GATC 1 cut(s) 134
MbiI CCGCTC 1 cut(s) 345
MboI GATC 1 cut(s) 132
MhlI GDGCHC 2 cut(s) 30, 223
MlyI GAGTC 2 cut(s) 71, 155
MnlI CCTC 2 cut(s) 41, 160
MroNI GCCGGC 1 cut(s) 174
MseI TTAA 1 cut(s) 225
MspCI CTTAAG 1 cut(s) 224
MspI CCGG 2 cut(s) 175, 255
MspR9I CCNGG 2 cut(s) 94, 255
MvaI CCWGG 1 cut(s) 94
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 2 cut(s) 50, 318
NaeI GCCGGC 1 cut(s) 176
NciI CCSGG 1 cut(s) 255
NcoI CCATGG 1 cut(s) 45
NdeII GATC 1 cut(s) 132
NgoMIV GCCGGC 1 cut(s) 174
NlaIII CATG 2 cut(s) 49, 133
NlaIV GGNNCC 2 cut(s) 220, 240
NmuCI GTSAC 1 cut(s) 125
PdiI GCCGGC 1 cut(s) 176
PkrI GCNGC 3 cut(s) 55, 91, 113
PleI GAGTC 2 cut(s) 70, 155
PpsI GAGTC 2 cut(s) 70, 155
Psp6I CCWGG 1 cut(s) 92
PspGI CCWGG 1 cut(s) 92
PspN4I GGNNCC 2 cut(s) 220, 240
PspPI GGNCC 1 cut(s) 37
PstI CTGCAG 1 cut(s) 58
RsaI GTAC 1 cut(s) 240
RsaNI GTAC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 225
SatI GCNGC 3 cut(s) 54, 90, 112
Sau3AI GATC 1 cut(s) 132
Sau96I GGNCC 1 cut(s) 37
SchI GAGTC 2 cut(s) 71, 155
ScrFI CCNGG 2 cut(s) 94, 255
SduI GDGCHC 2 cut(s) 30, 223
SetI ASST 4 cut(s) 123, 171, 215, 240
SfaNI GCATC 2 cut(s) 69, 269
SfcI CTRYAG 1 cut(s) 54
SmlI CTYRAG 1 cut(s) 224
SmoI CTYRAG 1 cut(s) 224
SsiI CCGC 4 cut(s) 90, 112, 310, 343
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 92, 253
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 2 cut(s) 218, 243
TaiI ACGT 1 cut(s) 123
TaqI TCGA 1 cut(s) 77
TauI GCSGC 2 cut(s) 92, 114
Tru1I TTAA 1 cut(s) 225
Tru9I TTAA 1 cut(s) 225
TseFI GTSAC 1 cut(s) 125
TseI GCWGC 1 cut(s) 53
Tsp45I GTSAC 1 cut(s) 125
TspGWI ACGGA 1 cut(s) 191
Vha464I CTTAAG 1 cut(s) 224
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.