Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0003104

Physical Location & Seq

Genomic Coordinates Forward (+)

38118842 .. 38119210

Gene Structure 369 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1292900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 369 bp

ATGGGATTCAAGCTCAACCTGATCGCCCTCATGGTGGTATTGGCCATGGTAGCTAGGACATCAGCCAAGGTCCACGATGCCTCGTGGGGTTTCAGCCGCTTCGGTGATCACGATGATGAGTCCTTGATCAATAGCGCCAACAGCAAAGTTGGCGACTTCATCGGAGACGAGAATGAGATGATGATGGAGTCAGAGAGCACTCGTAGGACACTCATAGGCAACGGCAGGTTTATTGGTTATTCTGCCCTAAAGCAAAATGCTGTTCCATGCGGACGCCGTGGCCAGTCCTACTACGACTGCCAAAAAAGGGATGGGGCTAACCCTTACAAACGGAGTTGTCAGTACATCACCCGCTGCGCCAGACGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0005102 GO:0005179 GO:0005488 GO:0005515 GO:0005575 GO:0005576 GO:0005623 GO:0005911 GO:0007154 GO:0007165 GO:0007267 GO:0008150 GO:0009506 GO:0009966 GO:0009987 GO:0010469 GO:0010646 GO:0010769 GO:0019722 GO:0019932 GO:0022603 GO:0022604 GO:0023051 GO:0023052 GO:0030054 GO:0030545 GO:0035556 GO:0040008 GO:0042995 GO:0043900 GO:0044464 GO:0045595 GO:0048018 GO:0048046 GO:0048583 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0055044 GO:0060284 GO:0065007 GO:0065009 GO:0080092 GO:0090406 GO:0098772 GO:0120025 GO:2000241

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

RALF

Protein Analysis

123

Amino Acids

13.71

Weight (kDa)

9.21

Isoelectric Point (pI)

40.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RALF	PF05498	59 - 121	1.5e-23	Rapid ALkalinization Factor (RALF)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0003104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G28270 AT2G33775 AT3G05490
fragaria_vesca	FvH4_3g09010 FvH4_3g09020
malus_domestica	MD00G1116200.v1.1 MD10G1292800.v1.1 MD10G1292900.v1.1
prunus_persica	Prupe.4G049000_v2.0.a1
pyrus_communis	pycom05g28940 pycom10g24490
rosa_chinensis	RchiOBHm_Chr5g0008651 RchiOBHm_Chr5g0008661 RchiOBHm_Chr5g0008671
rosa_laevigata	RLG00000031590 RLG00000031591 RLG00000031592
rosa_multiflora	Rmu_sc0001665.1_g000030 Rmu_sc0001665.1_g000031 Rmu_sc0001665.1_g000040
rosa_roxburghii	Rroxscaffold_1G00067890 Rroxscaffold_1G00067900 Rroxscaffold_1G00067910
rosa_rugosa	Rorug04G0437700 Rorug04G0437700 Rorug04G0437700
rosa_samantha	Rh5AG066300 Rh5BG063600 Rh5BG063700 Rh5CG073800 Rh5CG073900 Rh5CG074100 Rh5DG062500 Rh5DG062600
rosa_wichuraiana	Rw5G006230 Rw5G006260 Rw5G006270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	216
AciI	CCGC	3 cut(s)	97, 270, 352
AcoI	YGGCCR	2 cut(s)	42, 280
AcyI	GRCGYC	1 cut(s)	274
AfaI	GTAC	1 cut(s)	344
AfiI	CCNNNNNNNGG	2 cut(s)	34, 307
AgsI	TTSAA	1 cut(s)	10
AluBI	AGCT	2 cut(s)	13, 53
AluI	AGCT	2 cut(s)	13, 53
Alw21I	GWGCWC	1 cut(s)	200
Alw26I	GTCTC	1 cut(s)	159
AoxI	GGCC	2 cut(s)	42, 280
ApeKI	GCWGC	1 cut(s)	354
AspLEI	GCGC	2 cut(s)	137, 359
AspS9I	GGNCC	1 cut(s)	70
AsuHPI	GGTGA	2 cut(s)	116, 340
AvaII	GGWCC	1 cut(s)	70
BalI	TGGCCA	2 cut(s)	44, 282
BauI	CACGAG	1 cut(s)	82
Bbv12I	GWGCWC	1 cut(s)	200
BbvI	GCAGC	1 cut(s)	341
BccI	CCATC	2 cut(s)	178, 305
BceAI	ACGGC	2 cut(s)	238, 261
BclI	TGATCA	2 cut(s)	106, 126
BcoDI	GTCTC	1 cut(s)	159
BfaI	CTAG	1 cut(s)	54
BfoI	RGCGCY	1 cut(s)	138
BfuAI	ACCTGC	1 cut(s)	216
BisI	GCNGC	2 cut(s)	97, 355
BlsI	GCNGC	2 cut(s)	98, 356
Bme18I	GGWCC	1 cut(s)	70
BmgT120I	GGNCC	1 cut(s)	70
BmsI	GCATC	1 cut(s)	67
BsaHI	GRCGYC	1 cut(s)	274
BsaJI	CCNNGG	3 cut(s)	45, 66, 277
Bsc4I	CCNNNNNNNGG	2 cut(s)	34, 307
Bse1I	ACTGG	1 cut(s)	283
BseDI	CCNNGG	3 cut(s)	45, 66, 277
BseGI	GGATG	1 cut(s)	316
BseLI	CCNNNNNNNGG	2 cut(s)	34, 307
BseNI	ACTGG	1 cut(s)	283
BseXI	GCAGC	1 cut(s)	341
BshFI	GGCC	2 cut(s)	44, 282
BsiHKAI	GWGCWC	1 cut(s)	200
BslI	CCNNNNNNNGG	2 cut(s)	34, 307
BsmAI	GTCTC	1 cut(s)	159
BsmBI	CGTCTC	1 cut(s)	159
BsnI	GGCC	2 cut(s)	44, 282
Bsp1286I	GDGCHC	1 cut(s)	200
Bsp143I	GATC	3 cut(s)	21, 106, 126
Bsp19I	CCATGG	1 cut(s)	45
BspACI	CCGC	3 cut(s)	97, 270, 352
BspANI	GGCC	2 cut(s)	44, 282
BspMI	ACCTGC	1 cut(s)	216
BsrI	ACTGG	1 cut(s)	283
BssECI	CCNNGG	3 cut(s)	45, 66, 277
BssMI	GATC	3 cut(s)	21, 106, 126
BssNI	GRCGYC	1 cut(s)	274
BssSI	CACGAG	1 cut(s)	82
BssT1I	CCWWGG	2 cut(s)	45, 66
Bst2BI	CACGAG	1 cut(s)	82
BstACI	GRCGYC	1 cut(s)	274
BstDSI	CCRYGG	2 cut(s)	45, 277
BstF5I	GGATG	1 cut(s)	316
BstH2I	RGCGCY	1 cut(s)	138
BstHHI	GCGC	2 cut(s)	137, 359
BstKTI	GATC	3 cut(s)	24, 109, 129
BstMAI	GTCTC	1 cut(s)	159
BstMBI	GATC	3 cut(s)	21, 106, 126
BstMWI	GCNNNNNNNGC	4 cut(s)	50, 141, 150, 363
BstV1I	GCAGC	1 cut(s)	341
BsuRI	GGCC	2 cut(s)	44, 282
BtgI	CCRYGG	2 cut(s)	45, 277
BtsCI	GGATG	1 cut(s)	316
BveI	ACCTGC	1 cut(s)	216
CfoI	GCGC	2 cut(s)	137, 359
Cfr13I	GGNCC	1 cut(s)	70
CseI	GACGC	1 cut(s)	282
Csp6I	GTAC	1 cut(s)	343
CviAII	CATG	3 cut(s)	31, 46, 267
CviJI	RGCY	7 cut(s)	13, 44, 53, 65, 96, 282, 317
CviKI_1	RGCY	7 cut(s)	13, 44, 53, 65, 96, 282, 317
CviQI	GTAC	1 cut(s)	343
DpnI	GATC	3 cut(s)	23, 108, 128
DpnII	GATC	3 cut(s)	21, 106, 126
EaeI	YGGCCR	2 cut(s)	42, 280
Eco130I	CCWWGG	2 cut(s)	45, 66
Eco47I	GGWCC	1 cut(s)	70
EcoT14I	CCWWGG	2 cut(s)	45, 66
ErhI	CCWWGG	2 cut(s)	45, 66
Esp3I	CGTCTC	1 cut(s)	159
FaeI	CATG	3 cut(s)	34, 49, 270
FaiI	YATR	4 cut(s)	32, 47, 215, 268
FatI	CATG	3 cut(s)	30, 45, 266
FauI	CCCGC	1 cut(s)	359
FbaI	TGATCA	2 cut(s)	106, 126
Fnu4HI	GCNGC	2 cut(s)	97, 355
FokI	GGATG	1 cut(s)	323
Fsp4HI	GCNGC	2 cut(s)	97, 355
FspBI	CTAG	1 cut(s)	54
GlaI	GCGC	2 cut(s)	136, 358
GluI	GCNGC	2 cut(s)	97, 355
HaeII	RGCGCY	1 cut(s)	138
HaeIII	GGCC	2 cut(s)	44, 282
HgaI	GACGC	1 cut(s)	282
HhaI	GCGC	2 cut(s)	137, 359
Hin1I	GRCGYC	1 cut(s)	274
Hin1II	CATG	3 cut(s)	34, 49, 270
Hin6I	GCGC	2 cut(s)	135, 357
HinP1I	GCGC	2 cut(s)	135, 357
HinfI	GANTC	3 cut(s)	6, 119, 188
HphI	GGTGA	2 cut(s)	116, 340
Hpy166II	GTNNAC	1 cut(s)	73
Hpy188I	TCNGA	2 cut(s)	164, 193
Hpy188III	TCNNGA	1 cut(s)	110
Hpy8I	GTNNAC	1 cut(s)	73
HpyF10VI	GCNNNNNNNGC	4 cut(s)	50, 141, 150, 363
Hsp92I	GRCGYC	1 cut(s)	274
Hsp92II	CATG	3 cut(s)	34, 49, 270
HspAI	GCGC	2 cut(s)	135, 357
Ksp22I	TGATCA	2 cut(s)	106, 126
Kzo9I	GATC	3 cut(s)	21, 106, 126
LpnPI	CCDG	3 cut(s)	32, 211, 296
Lsp1109I	GCAGC	1 cut(s)	341
LweI	GCATC	1 cut(s)	67
MaeI	CTAG	1 cut(s)	54
MalI	GATC	3 cut(s)	23, 108, 128
MboI	GATC	3 cut(s)	21, 106, 126
MhlI	GDGCHC	1 cut(s)	200
MlsI	TGGCCA	2 cut(s)	44, 282
MluNI	TGGCCA	2 cut(s)	44, 282
MlyI	GAGTC	2 cut(s)	128, 197
MnlI	CCTC	2 cut(s)	38, 91
Mox20I	TGGCCA	2 cut(s)	44, 282
MscI	TGGCCA	2 cut(s)	44, 282
MslI	CAYNNNNRTG	1 cut(s)	114
Msp20I	TGGCCA	2 cut(s)	44, 282
MspA1I	CMGCKG	1 cut(s)	354
MwoI	GCNNNNNNNGC	4 cut(s)	50, 141, 150, 363
NcoI	CCATGG	1 cut(s)	45
NdeII	GATC	3 cut(s)	21, 106, 126
NlaIII	CATG	3 cut(s)	34, 49, 270
PcsI	WCGNNNNNNNCGW	1 cut(s)	108
PfeI	GAWTC	1 cut(s)	6
PkrI	GCNGC	2 cut(s)	98, 356
PleI	GAGTC	2 cut(s)	127, 196
PpsI	GAGTC	2 cut(s)	127, 196
PspPI	GGNCC	1 cut(s)	70
RsaI	GTAC	1 cut(s)	344
RsaNI	GTAC	1 cut(s)	343
RseI	CAYNNNNRTG	1 cut(s)	114
SatI	GCNGC	2 cut(s)	97, 355
Sau3AI	GATC	3 cut(s)	21, 106, 126
Sau96I	GGNCC	1 cut(s)	70
SchI	GAGTC	2 cut(s)	128, 197
SduI	GDGCHC	1 cut(s)	200
SetI	ASST	5 cut(s)	15, 21, 55, 72, 230
SfaNI	GCATC	1 cut(s)	67
SinI	GGWCC	1 cut(s)	70
SmiMI	CAYNNNNRTG	1 cut(s)	114
SsiI	CCGC	3 cut(s)	97, 270, 352
SspMI	CTAG	1 cut(s)	54
StyI	CCWWGG	2 cut(s)	45, 66
TatI	WGTACW	1 cut(s)	342
TauI	GCSGC	1 cut(s)	99
TfiI	GAWTC	1 cut(s)	6
TseI	GCWGC	1 cut(s)	354
TspDTI	ATGAA	1 cut(s)	148
TspGWI	ACGGA	1 cut(s)	346
VpaK11BI	GGWCC	1 cut(s)	70
XcmI	CCANNNNNNNNNTGG	1 cut(s)	308
XspI	CTAG	1 cut(s)	54

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.