MD00G1116200.v1.1

Rapid ALkalinization Factor (RALF)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0003104
Physical Location & Seq
Forward (+)
24576261 .. 24576632
372 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1116200.v1.1.491

Sequence Viewer

Length: 372 bp
ATGGGATTCAAGCTCTACCTGATCGCCCTCATGGTGGTCTTGGCCATGGTGGCTAGGTCATCGGCCAAGGTCCACGATGCCTTGTGGACTCTCAGCCACTTCGGCAATCACGATGATGAGTCGTTGCTCGACAGCGCCAACAGCAAAGTCGGTGACTTGATCGGAGAGGAGAATGAGATGATGATGGAGACAGAGAGCACTCGTAGGACACTCAGAGGCCGCGGCAGGAGGTACGTTGGCTATGGTGCCCTGAGACGCAATGCTGTTCCATGCGGACGCCGCGGTCGGTCCTACTACAACTGCCAAGGAAGGCAGAGGGCTAACCCTTACAAGCGCGGTTGCCAGTACATCACCCGCTGCGCCAGACGCTAA

Protein Analysis

124

Amino Acids

14.06

Weight (kDa)

10.09

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RALF PF05498 59 - 122 4.6e-25 Rapid ALkalinization Factor (RALF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 282
AccB1I GGYRCC 1 cut(s) 245
AccII CGCG 3 cut(s) 222, 282, 336
AciI CCGC 7 cut(s) 220, 222, 273, 280, 282, 336, 355
AcoI YGGCCR 2 cut(s) 42, 63
AcyI GRCGYC 1 cut(s) 277
AfaI GTAC 2 cut(s) 233, 347
AfiI CCNNNNNNNGG 1 cut(s) 34
AgsI TTSAA 1 cut(s) 10
AluBI AGCT 1 cut(s) 13
AluI AGCT 1 cut(s) 13
Alw21I GWGCWC 1 cut(s) 200
Alw26I GTCTC 2 cut(s) 182, 247
AoxI GGCC 3 cut(s) 42, 63, 217
ApeKI GCWGC 1 cut(s) 357
AspLEI GCGC 3 cut(s) 137, 336, 362
AspS9I GGNCC 2 cut(s) 70, 288
AsuHPI GGTGA 2 cut(s) 164, 343
AvaII GGWCC 2 cut(s) 70, 288
BaeGI GKGCMC 1 cut(s) 250
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 245
Bbv12I GWGCWC 1 cut(s) 200
BbvI GCAGC 1 cut(s) 344
BccI CCATC 1 cut(s) 178
BcoDI GTCTC 2 cut(s) 182, 247
BfaI CTAG 1 cut(s) 54
BfoI RGCGCY 1 cut(s) 138
BglI GCCNNNNNGGC 2 cut(s) 50, 102
BisI GCNGC 4 cut(s) 220, 223, 280, 358
BlsI GCNGC 4 cut(s) 221, 224, 281, 359
Bme18I GGWCC 2 cut(s) 70, 288
BmgT120I GGNCC 2 cut(s) 70, 288
BmiI GGNNCC 1 cut(s) 247
BmsI GCATC 1 cut(s) 67
BsaHI GRCGYC 1 cut(s) 277
BsaJI CCNNGG 5 cut(s) 45, 66, 220, 280, 304
Bsc4I CCNNNNNNNGG 1 cut(s) 34
Bse1I ACTGG 1 cut(s) 343
Bse3DI GCAATG 1 cut(s) 265
BseDI CCNNGG 5 cut(s) 45, 66, 220, 280, 304
BseLI CCNNNNNNNGG 1 cut(s) 34
BseMI GCAATG 1 cut(s) 265
BseMII CTCAG 3 cut(s) 106, 226, 242
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 1 cut(s) 182
BseSI GKGCMC 1 cut(s) 250
BseXI GCAGC 1 cut(s) 344
Bsh1236I CGCG 3 cut(s) 222, 282, 336
Bsh1285I CGRYCG 1 cut(s) 286
BshFI GGCC 3 cut(s) 44, 65, 219
BshNI GGYRCC 1 cut(s) 245
BsiEI CGRYCG 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 34
BsmAI GTCTC 2 cut(s) 182, 247
BsmBI CGTCTC 1 cut(s) 247
BsnI GGCC 3 cut(s) 44, 65, 219
Bsp1286I GDGCHC 2 cut(s) 200, 250
Bsp143I GATC 2 cut(s) 21, 159
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 7 cut(s) 220, 222, 273, 280, 282, 336, 355
BspANI GGCC 3 cut(s) 44, 65, 219
BspCNI CTCAG 3 cut(s) 105, 225, 243
BspFNI CGCG 3 cut(s) 222, 282, 336
BspLI GGNNCC 1 cut(s) 247
BspT107I GGYRCC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 265
BsrI ACTGG 1 cut(s) 343
BssECI CCNNGG 5 cut(s) 45, 66, 220, 280, 304
BssMI GATC 2 cut(s) 21, 159
BssNI GRCGYC 1 cut(s) 277
BssT1I CCWWGG 3 cut(s) 45, 66, 304
BstACI GRCGYC 1 cut(s) 277
BstDEI CTNAG 3 cut(s) 92, 212, 251
BstDSI CCRYGG 3 cut(s) 45, 220, 280
BstFNI CGCG 3 cut(s) 222, 282, 336
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 3 cut(s) 137, 336, 362
BstKTI GATC 2 cut(s) 24, 162
BstMAI GTCTC 2 cut(s) 182, 247
BstMBI GATC 2 cut(s) 21, 159
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 5 cut(s) 50, 102, 141, 279, 366
BstSLI GKGCMC 1 cut(s) 250
BstUI CGCG 3 cut(s) 222, 282, 336
BstV1I GCAGC 1 cut(s) 344
BsuRI GGCC 3 cut(s) 44, 65, 219
BtgI CCRYGG 3 cut(s) 45, 220, 280
CfoI GCGC 3 cut(s) 137, 336, 362
Cfr13I GGNCC 2 cut(s) 70, 288
Cfr42I CCGCGG 2 cut(s) 223, 283
CseI GACGC 2 cut(s) 264, 285
Csp6I GTAC 2 cut(s) 232, 346
CviAII CATG 3 cut(s) 31, 46, 270
CviJI RGCY 8 cut(s) 13, 44, 53, 65, 96, 219, 240, 320
CviKI_1 RGCY 8 cut(s) 13, 44, 53, 65, 96, 219, 240, 320
CviQI GTAC 2 cut(s) 232, 346
DdeI CTNAG 3 cut(s) 92, 212, 251
DpnI GATC 2 cut(s) 23, 161
DpnII GATC 2 cut(s) 21, 159
DrdI GACNNNNNNGTC 1 cut(s) 282
DseDI GACNNNNNNGTC 1 cut(s) 282
EaeI YGGCCR 2 cut(s) 42, 63
Eco130I CCWWGG 3 cut(s) 45, 66, 304
Eco47I GGWCC 2 cut(s) 70, 288
EcoT14I CCWWGG 3 cut(s) 45, 66, 304
ErhI CCWWGG 3 cut(s) 45, 66, 304
Esp3I CGTCTC 1 cut(s) 247
FaeI CATG 3 cut(s) 34, 49, 273
FaiI YATR 4 cut(s) 32, 47, 243, 271
FatI CATG 3 cut(s) 30, 45, 269
FauI CCCGC 1 cut(s) 362
Fnu4HI GCNGC 4 cut(s) 220, 223, 280, 358
Fsp4HI GCNGC 4 cut(s) 220, 223, 280, 358
FspBI CTAG 1 cut(s) 54
GlaI GCGC 3 cut(s) 136, 335, 361
GluI GCNGC 4 cut(s) 220, 223, 280, 358
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 3 cut(s) 44, 65, 219
HgaI GACGC 2 cut(s) 264, 285
HhaI GCGC 3 cut(s) 137, 336, 362
Hin1I GRCGYC 1 cut(s) 277
Hin1II CATG 3 cut(s) 34, 49, 273
Hin6I GCGC 3 cut(s) 135, 334, 360
HinP1I GCGC 3 cut(s) 135, 334, 360
HinfI GANTC 3 cut(s) 6, 88, 119
HphI GGTGA 2 cut(s) 164, 343
Hpy166II GTNNAC 2 cut(s) 73, 87
Hpy188I TCNGA 2 cut(s) 164, 215
Hpy188III TCNNGA 1 cut(s) 110
Hpy8I GTNNAC 2 cut(s) 73, 87
HpyAV CCTTC 1 cut(s) 303
HpyCH4IV ACGT 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 102, 141, 279, 366
HpyF3I CTNAG 3 cut(s) 92, 212, 251
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 1 cut(s) 277
Hsp92II CATG 3 cut(s) 34, 49, 273
HspAI GCGC 3 cut(s) 135, 334, 360
KspI CCGCGG 2 cut(s) 223, 283
Kzo9I GATC 2 cut(s) 21, 159
LpnPI CCDG 4 cut(s) 32, 211, 263, 356
Lsp1109I GCAGC 1 cut(s) 344
LweI GCATC 1 cut(s) 67
MaeI CTAG 1 cut(s) 54
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 152
MalI GATC 2 cut(s) 23, 161
MboI GATC 2 cut(s) 21, 159
MhlI GDGCHC 2 cut(s) 200, 250
MlsI TGGCCA 1 cut(s) 44
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 2 cut(s) 82, 128
MnlI CCTC 5 cut(s) 38, 160, 209, 222, 309
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MslI CAYNNNNRTG 1 cut(s) 114
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 3 cut(s) 222, 282, 357
MvnI CGCG 3 cut(s) 222, 282, 336
MwoI GCNNNNNNNGC 5 cut(s) 50, 102, 141, 279, 366
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 21, 159
NlaIII CATG 3 cut(s) 34, 49, 273
NlaIV GGNNCC 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 1 cut(s) 6
PkrI GCNGC 4 cut(s) 221, 224, 281, 359
PleI GAGTC 2 cut(s) 82, 127
PpsI GAGTC 2 cut(s) 82, 127
PspN4I GGNNCC 1 cut(s) 247
PspPI GGNCC 2 cut(s) 70, 288
RsaI GTAC 2 cut(s) 233, 347
RsaNI GTAC 2 cut(s) 232, 346
RseI CAYNNNNRTG 1 cut(s) 114
SacII CCGCGG 2 cut(s) 223, 283
SatI GCNGC 4 cut(s) 220, 223, 280, 358
Sau3AI GATC 2 cut(s) 21, 159
Sau96I GGNCC 2 cut(s) 70, 288
SchI GAGTC 2 cut(s) 82, 128
SduI GDGCHC 2 cut(s) 200, 250
SetI ASST 6 cut(s) 15, 21, 59, 72, 233, 237
SfaNI GCATC 1 cut(s) 67
Sfr303I CCGCGG 2 cut(s) 223, 283
SgrBI CCGCGG 2 cut(s) 223, 283
SinI GGWCC 2 cut(s) 70, 288
SmiMI CAYNNNNRTG 1 cut(s) 114
SsiI CCGC 7 cut(s) 220, 222, 273, 280, 282, 336, 355
SspMI CTAG 1 cut(s) 54
StyI CCWWGG 3 cut(s) 45, 66, 304
TaiI ACGT 1 cut(s) 237
TaqI TCGA 1 cut(s) 129
TaqII GACCGA 1 cut(s) 276
TatI WGTACW 1 cut(s) 345
TauI GCSGC 3 cut(s) 222, 225, 282
TfiI GAWTC 1 cut(s) 6
TseFI GTSAC 1 cut(s) 152
TseI GCWGC 1 cut(s) 357
Tsp45I GTSAC 1 cut(s) 152
VpaK11BI GGWCC 2 cut(s) 70, 288
XspI CTAG 1 cut(s) 54
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.