AT2G35990
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011648
Physical Location & Seq
Forward (+)
15112084 .. 15116776
4693 bp
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UTR
Exon/CDS
Intron
AT2G35990.2

Sequence Viewer

Length: 642 bp
ATGGAAGAGACAAAATCAAGATTCAGGAGGATCTGTGTCTTCTGCGGAAGCAGTTCCGGCAACAAAACCACTTATCACGACGCTGCTCTCCAACTCGCCCACCAACTGGTGGAGAGAAACATCGATTTGGTGTATGGAGGAGGAAGCGTGGGGCTAATGGGTCTCATTTCTCAAGCTGTTCATGATGGTGGTCGTCATGTTCTTGGGATCATTCCGAAGTCTCTAGCACCAAGAGAGATAACCGGTGAATCAATAGGAGAAGTTATAACAGTATCGACTATGCACCAAAGGAAGGCTGAAATGGGTCGCCAAGCGGATGCCTTCATCGCACTTCCTGGTGGATATGGGACATTTGAAGAGTTGTTGGAAGTCATCACCTGGTCTCAGCTTGGGATTCACACTAAACCGGTGGGACTATTGAACGTGGATGGATTCTACGATTCACTATTGACGTTTATAGATAAGGCAGTGGACGAAGGCTTCGTCTCCTCCACCGCTCGCCGTATCATTGTCTCGGCTCCAAACGCTCCTCAATTACTTCAACTTCTTGAGGAGTATGTTCCAAAACACGACGATTTTGTATCCAAAATGGTGTGGGACAATACCACGGATGCTTTCACTTTGGAAGGCGACTCGTTTTAG

Protein Analysis

213

Amino Acids

23.31

Weight (kDa)

5.2

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lysine_decarbox PF03641 1 - 130 1.1e-48 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0011648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35990 AT2G35990 AT2G35990 AT5G06300
fragaria_vesca FvH4_6g20040 FvH4_6g20040 FvH4_6g20040
malus_domestica MD12G1037500.v1.1 MD14G1036500.v1.1
prunus_persica Prupe.7G097700_v2.0.a1 Prupe.7G097700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475501
rosa_laevigata RLG00000023840
rosa_multiflora Rmu_sc0000539.1_g000004
rosa_roxburghii Rroxscaffold_6G00406230
rosa_rugosa Rorug03G0149300 Rorug03G0149400
rosa_samantha Rh3BG229300
rosa_wichuraiana Rw3G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 266
AccB7I CCANNNNNTGG 2 cut(s) 106, 109
AccBSI CCGCTC 1 cut(s) 497
AciI CCGC 3 cut(s) 45, 314, 495
AclWI GGATC 2 cut(s) 38, 215
AfiI CCNNNNNNNGG 3 cut(s) 106, 109, 292
AgeI ACCGGT 2 cut(s) 242, 406
AgsI TTSAA 3 cut(s) 356, 421, 542
AjnI CCWGG 2 cut(s) 334, 377
AluBI AGCT 2 cut(s) 176, 388
AluI AGCT 2 cut(s) 176, 388
Alw26I GTCTC 6 cut(s) 2, 167, 225, 387, 490, 517
AlwI GGATC 2 cut(s) 38, 215
ApeKI GCWGC 1 cut(s) 83
ArsI GACNNNNNNTTYG 4 cut(s) 464, 468, 496, 500
AsiGI ACCGGT 2 cut(s) 242, 406
Asp700I GAANNNNTTC 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 257, 367
BbsI GAAGAC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 70
BccI CCATC 2 cut(s) 179, 422
BceAI ACGGC 1 cut(s) 486
BciT130I CCWGG 2 cut(s) 336, 379
BciVI GTATCC 1 cut(s) 592
BcoDI GTCTC 6 cut(s) 2, 167, 225, 387, 490, 517
BfaI CTAG 1 cut(s) 224
BfuI GTATCC 1 cut(s) 592
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 336, 379
BmiI GGNNCC 1 cut(s) 519
BmrFI CCNGG 2 cut(s) 336, 379
BmsI GCATC 2 cut(s) 307, 601
BpiI GAAGAC 1 cut(s) 31
BpuEI CTTGAG 2 cut(s) 156, 569
Bsa29I ATCGAT 1 cut(s) 123
BsaI GGTCTC 2 cut(s) 167, 387
BsaJI CCNNGG 1 cut(s) 606
BsaWI WCCGGW 2 cut(s) 242, 406
BsaXI ACNNNNNCTCC 4 cut(s) 19, 49, 132, 162
Bsc4I CCNNNNNNNGG 3 cut(s) 106, 109, 292
Bse118I RCCGGY 2 cut(s) 242, 406
Bse1I ACTGG 1 cut(s) 111
BseBI CCWGG 2 cut(s) 336, 379
BseCI ATCGAT 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 606
BseGI GGATG 3 cut(s) 322, 433, 616
BseLI CCNNNNNNNGG 3 cut(s) 106, 109, 292
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 1 cut(s) 111
BseRI GAGGAG 4 cut(s) 153, 478, 519, 566
BseXI GCAGC 1 cut(s) 70
BshTI ACCGGT 2 cut(s) 242, 406
BshVI ATCGAT 1 cut(s) 123
BsiSI CCGG 3 cut(s) 57, 243, 407
BslFI GGGAC 3 cut(s) 361, 426, 611
BslI CCNNNNNNNGG 3 cut(s) 106, 109, 292
BsmAI GTCTC 6 cut(s) 2, 167, 225, 387, 490, 517
BsmBI CGTCTC 1 cut(s) 490
BsmFI GGGAC 3 cut(s) 361, 426, 611
Bso31I GGTCTC 2 cut(s) 167, 387
Bsp143I GATC 2 cut(s) 30, 207
BspACI CCGC 3 cut(s) 45, 314, 495
BspCNI CTCAG 1 cut(s) 397
BspDI ATCGAT 1 cut(s) 123
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 519
BspPI GGATC 2 cut(s) 38, 215
BspTNI GGTCTC 2 cut(s) 167, 387
BsrBI CCGCTC 1 cut(s) 497
BsrFI RCCGGY 2 cut(s) 242, 406
BsrI ACTGG 1 cut(s) 111
BssAI RCCGGY 2 cut(s) 242, 406
BssECI CCNNGG 1 cut(s) 606
BssMI GATC 2 cut(s) 30, 207
Bst2UI CCWGG 2 cut(s) 336, 379
Bst4CI ACNGT 1 cut(s) 271
Bst6I CTCTTC 1 cut(s) 351
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 1 cut(s) 384
BstDSI CCRYGG 1 cut(s) 606
BstF5I GGATG 3 cut(s) 322, 433, 616
BstKTI GATC 2 cut(s) 33, 210
BstMAI GTCTC 6 cut(s) 2, 167, 225, 387, 490, 517
BstMBI GATC 2 cut(s) 30, 207
BstMWI GCNNNNNNNGC 3 cut(s) 57, 326, 524
BstNI CCWGG 2 cut(s) 336, 379
BstSCI CCNGG 2 cut(s) 334, 377
BstV1I GCAGC 1 cut(s) 70
BstV2I GAAGAC 1 cut(s) 31
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
Bsu15I ATCGAT 1 cut(s) 123
BsuI GTATCC 1 cut(s) 592
BsuTUI ATCGAT 1 cut(s) 123
BtgI CCRYGG 1 cut(s) 606
BtgZI GCGATG 1 cut(s) 310
BtsCI GGATG 3 cut(s) 322, 433, 616
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 1 cut(s) 474
Cac8I GCNNGC 1 cut(s) 499
CciI TCATGA 1 cut(s) 181
Cfr10I RCCGGY 2 cut(s) 242, 406
ClaI ATCGAT 1 cut(s) 123
CseI GACGC 1 cut(s) 89
CsiI ACCWGGT 1 cut(s) 377
CspAI ACCGGT 2 cut(s) 242, 406
CviAII CATG 2 cut(s) 182, 197
CviJI RGCY 6 cut(s) 154, 176, 296, 388, 480, 518
CviKI_1 RGCY 6 cut(s) 154, 176, 296, 388, 480, 518
DdeI CTNAG 1 cut(s) 384
DpnI GATC 2 cut(s) 32, 209
DpnII GATC 2 cut(s) 30, 207
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Eco31I GGTCTC 2 cut(s) 167, 387
EcoRII CCWGG 2 cut(s) 334, 377
Esp3I CGTCTC 1 cut(s) 490
FaeI CATG 2 cut(s) 185, 200
FaiI YATR 8 cut(s) 135, 183, 198, 266, 281, 345, 458, 558
FaqI GGGAC 3 cut(s) 361, 426, 611
FatI CATG 2 cut(s) 181, 196
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 3 cut(s) 329, 440, 623
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 1 cut(s) 224
GluI GCNGC 1 cut(s) 84
HapII CCGG 3 cut(s) 57, 243, 407
HgaI GACGC 1 cut(s) 89
Hin1II CATG 2 cut(s) 185, 200
HinfI GANTC 6 cut(s) 21, 248, 394, 432, 440, 632
HpaII CCGG 3 cut(s) 57, 243, 407
HphI GGTGA 2 cut(s) 257, 367
Hpy166II GTNNAC 1 cut(s) 472
Hpy188I TCNGA 1 cut(s) 216
Hpy188III TCNNGA 5 cut(s) 18, 25, 77, 182, 548
Hpy8I GTNNAC 1 cut(s) 472
Hpy99I CGWCG 2 cut(s) 83, 575
HpyAV CCTTC 4 cut(s) 286, 331, 470, 620
HpyCH4III ACNGT 1 cut(s) 271
HpyCH4IV ACGT 2 cut(s) 423, 452
HpyCH4V TGCA 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 3 cut(s) 57, 326, 524
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 2 cut(s) 423, 452
Hsp92II CATG 2 cut(s) 185, 200
Kzo9I GATC 2 cut(s) 30, 207
LmnI GCTCC 2 cut(s) 523, 532
LpnPI CCDG 9 cut(s) 10, 70, 92, 256, 321, 348, 364, 391, 420
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 2 cut(s) 307, 601
MabI ACCWGGT 1 cut(s) 377
MaeI CTAG 1 cut(s) 224
MaeII ACGT 2 cut(s) 423, 452
MalI GATC 2 cut(s) 32, 209
MbiI CCGCTC 1 cut(s) 497
MboI GATC 2 cut(s) 30, 207
MboII GAAGA 3 cut(s) 17, 31, 368
MflI RGATCY 1 cut(s) 30
MluCI AATT 1 cut(s) 533
MlyI GAGTC 1 cut(s) 626
MmeI TCCRAC 2 cut(s) 115, 345
MnlI CCTC 6 cut(s) 21, 131, 134, 499, 540, 544
MroXI GAANNNNTTC 1 cut(s) 52
MslI CAYNNNNRTG 1 cut(s) 186
MspI CCGG 3 cut(s) 57, 243, 407
MspR9I CCNGG 2 cut(s) 336, 379
MvaI CCWGG 2 cut(s) 336, 379
MwoI GCNNNNNNNGC 3 cut(s) 57, 326, 524
NdeII GATC 2 cut(s) 30, 207
NlaIII CATG 2 cut(s) 185, 200
NlaIV GGNNCC 1 cut(s) 519
NmeAIII GCCGAG 1 cut(s) 494
PagI TCATGA 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PdmI GAANNNNTTC 1 cut(s) 52
PfeI GAWTC 5 cut(s) 21, 248, 394, 432, 440
PflMI CCANNNNNTGG 2 cut(s) 106, 109
PinAI ACCGGT 2 cut(s) 242, 406
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 1 cut(s) 626
PpsI GAGTC 1 cut(s) 626
PsiI TTATAA 1 cut(s) 266
Psp6I CCWGG 2 cut(s) 334, 377
PspGI CCWGG 2 cut(s) 334, 377
PspN4I GGNNCC 1 cut(s) 519
PsuI RGATCY 1 cut(s) 30
RseI CAYNNNNRTG 1 cut(s) 186
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 30, 207
SchI GAGTC 1 cut(s) 626
ScrFI CCNGG 2 cut(s) 336, 379
SetI ASST 5 cut(s) 178, 380, 390, 426, 455
SexAI ACCWGGT 1 cut(s) 377
SfaNI GCATC 2 cut(s) 307, 601
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 2 cut(s) 171, 548
SmoI CTYRAG 2 cut(s) 171, 548
Sse9I AATT 1 cut(s) 533
SsiI CCGC 3 cut(s) 45, 314, 495
SspMI CTAG 1 cut(s) 224
StyD4I CCNGG 2 cut(s) 334, 377
TaaI ACNGT 1 cut(s) 271
TaiI ACGT 2 cut(s) 426, 455
TaqI TCGA 2 cut(s) 123, 275
TasI AATT 1 cut(s) 533
TfiI GAWTC 5 cut(s) 21, 248, 394, 432, 440
TscAI CASTG 1 cut(s) 474
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 2 cut(s) 170, 313
TspGWI ACGGA 1 cut(s) 623
TspRI CASTG 1 cut(s) 474
Van91I CCANNNNNTGG 2 cut(s) 106, 109
XmnI GAANNNNTTC 1 cut(s) 52
XspI CTAG 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.