MD14G1036500.v1.1
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011648
Physical Location & Seq
Reverse (-)
3311141 .. 3315590
4450 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1036500.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGAAGAGACAAAGTCGAGATTCAATAGGATTTGTGTTTTCTGTGGTAGCAGTTCAGGGAAGAAAGCCAGCTACCAAGAAGCTGCTGTCGAACTTGGGAAGGAGCTGGTGGAGAGAAGGATTGATCTGGTCTATGGTGGTGGAAGCGTGGGATTGATGGGTCTTGTTTCTCAGGCAGTTCATGATGGCGGGCGCCATGTCCTAGGAGTTATTCCAAGGACCCTAATGCCTATAGAGATAACTGGTGAAACTGTTGGAGAAGTGAGAACTGTCTCAGATATGCACCAAAGGAAAGCCGAGATGGCCCGTCAAGCTGATGCTTTTATTGCCCTCCCTGGTGGCTATGGTACCCTGGAAGAATTGCTGGAAGTCATTACCTGGGCTCAGCTTGGAATCCACCGCAAACCTGTGGGGCTTTTGAATGTGGACGGTTACTACAATTCGTTGTTGTGTTTCATCGATAAGGCCGTTGATGAAGGCTTTATTTCTCCTACTGCACGTCACATTATCGTGTCTGCTCCAACAGCCAAGCAATTGGTCAGACAACTGGAGGAATATGTACCAGAACACGATGAAGTAACATCAAAGTTGGTGTGGGAAGAGGTGGACAGAATAAGCTATGTATCTGAACCAGGAGTGGCCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

216

Amino Acids

23.6

Weight (kDa)

5.3

Isoelectric Point (pI)

36.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 16 - 141 1e-13 SLOG cluster4 family
Lysine_decarbox PF03641 53 - 183 3.3e-49 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35990 AT2G35990 AT2G35990 AT5G06300
fragaria_vesca FvH4_6g20040 FvH4_6g20040 FvH4_6g20040
malus_domestica MD12G1037500.v1.1 MD14G1036500.v1.1
prunus_persica Prupe.7G097700_v2.0.a1 Prupe.7G097700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475501
rosa_laevigata RLG00000023840
rosa_multiflora Rmu_sc0000539.1_g000004
rosa_roxburghii Rroxscaffold_6G00406230
rosa_rugosa Rorug03G0149300 Rorug03G0149400
rosa_samantha Rh3BG229300
rosa_wichuraiana Rw3G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 347
AccB1I GGYRCC 2 cut(s) 192, 347
AciI CCGC 2 cut(s) 189, 400
AcoI YGGCCR 1 cut(s) 639
AcvI CACGTG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 2 cut(s) 349, 561
AgsI TTSAA 2 cut(s) 25, 421
AjiI CACGTC 1 cut(s) 500
AjnI CCWGG 4 cut(s) 334, 351, 377, 631
AluBI AGCT 6 cut(s) 72, 83, 106, 314, 388, 618
AluI AGCT 6 cut(s) 72, 83, 106, 314, 388, 618
Alw26I GTCTC 2 cut(s) 2, 277
AoxI GGCC 3 cut(s) 303, 465, 639
ApeKI GCWGC 1 cut(s) 83
Asp718I GGTACC 1 cut(s) 347
AspA2I CCTAGG 1 cut(s) 202
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 2 cut(s) 219, 304
AsuHPI GGTGA 1 cut(s) 257
AvaII GGWCC 1 cut(s) 219
AvrII CCTAGG 1 cut(s) 202
BalI TGGCCA 1 cut(s) 641
BanI GGYRCC 2 cut(s) 192, 347
BanII GRGCYC 1 cut(s) 385
BbrPI CACGTG 1 cut(s) 645
BbvI GCAGC 1 cut(s) 70
BccI CCATC 3 cut(s) 151, 179, 295
BceAI ACGGC 1 cut(s) 452
BciT130I CCWGG 4 cut(s) 336, 353, 379, 633
BcoDI GTCTC 2 cut(s) 2, 277
BfaI CTAG 1 cut(s) 203
BfmI CTRYAG 1 cut(s) 231
BfoI RGCGCY 1 cut(s) 196
BglI GCCNNNNNGGC 1 cut(s) 302
BisI GCNGC 1 cut(s) 84
BlnI CCTAGG 1 cut(s) 202
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 4 cut(s) 336, 353, 379, 633
Bme18I GGWCC 1 cut(s) 219
BmgBI CACGTC 1 cut(s) 500
BmgT120I GGNCC 2 cut(s) 219, 304
BmiI GGNNCC 3 cut(s) 194, 221, 349
BmrFI CCNGG 4 cut(s) 336, 353, 379, 633
BmsI GCATC 1 cut(s) 307
BpmI CTGGAG 1 cut(s) 569
Bpu1102I GCTNAGC 1 cut(s) 384
Bsa29I ATCGAT 1 cut(s) 459
BsaAI YACGTR 1 cut(s) 645
BsaHI GRCGYC 1 cut(s) 193
BsaJI CCNNGG 5 cut(s) 202, 215, 334, 351, 378
Bse1I ACTGG 2 cut(s) 247, 552
BseBI CCWGG 4 cut(s) 336, 353, 379, 633
BseCI ATCGAT 1 cut(s) 459
BseDI CCNNGG 5 cut(s) 202, 215, 334, 351, 378
BseMII CTCAG 3 cut(s) 185, 288, 398
BseNI ACTGG 2 cut(s) 247, 552
BseXI GCAGC 1 cut(s) 70
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 3 cut(s) 305, 467, 641
BshNI GGYRCC 2 cut(s) 192, 347
BshVI ATCGAT 1 cut(s) 459
BsmAI GTCTC 2 cut(s) 2, 277
BsnI GGCC 3 cut(s) 305, 467, 641
Bsp1286I GDGCHC 1 cut(s) 385
Bsp143I GATC 1 cut(s) 124
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 2 cut(s) 189, 400
BspANI GGCC 3 cut(s) 305, 467, 641
BspCNI CTCAG 3 cut(s) 184, 287, 397
BspDI ATCGAT 1 cut(s) 459
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 3 cut(s) 194, 221, 349
BspT107I GGYRCC 2 cut(s) 192, 347
BsrI ACTGG 2 cut(s) 247, 552
BssECI CCNNGG 5 cut(s) 202, 215, 334, 351, 378
BssMI GATC 1 cut(s) 124
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 2 cut(s) 202, 215
Bst2UI CCWGG 4 cut(s) 336, 353, 379, 633
Bst4CI ACNGT 3 cut(s) 253, 271, 431
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 193
BstBAI YACGTR 1 cut(s) 645
BstC8I GCNNGC 2 cut(s) 70, 191
BstDEI CTNAG 3 cut(s) 171, 274, 384
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 2 cut(s) 2, 277
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 4 cut(s) 302, 311, 326, 524
BstNI CCWGG 4 cut(s) 336, 353, 379, 633
BstSCI CCNGG 4 cut(s) 334, 351, 377, 631
BstSFI CTRYAG 1 cut(s) 231
BstV1I GCAGC 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 535
Bsu15I ATCGAT 1 cut(s) 459
BsuRI GGCC 3 cut(s) 305, 467, 641
BsuTUI ATCGAT 1 cut(s) 459
BtrI CACGTC 1 cut(s) 500
Cac8I GCNNGC 2 cut(s) 70, 191
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 2 cut(s) 219, 304
ClaI ATCGAT 1 cut(s) 459
Csp6I GTAC 2 cut(s) 348, 560
CviAII CATG 2 cut(s) 182, 197
CviQI GTAC 2 cut(s) 348, 560
DdeI CTNAG 3 cut(s) 171, 274, 384
DinI GGCGCC 1 cut(s) 194
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 639
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 202, 215
Eco24I GRGCYC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 219
Eco72I CACGTG 1 cut(s) 645
EcoO109I RGGNCCY 1 cut(s) 219
EcoRII CCWGG 4 cut(s) 334, 351, 377, 631
EcoT14I CCWWGG 2 cut(s) 202, 215
EcoT38I GRGCYC 1 cut(s) 385
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
ErhI CCWWGG 2 cut(s) 202, 215
FaeI CATG 2 cut(s) 185, 200
FaiI YATR 8 cut(s) 135, 183, 198, 233, 281, 345, 558, 621
FatI CATG 2 cut(s) 181, 196
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 1 cut(s) 84
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 1 cut(s) 203
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 569
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 3 cut(s) 305, 467, 641
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 2 cut(s) 185, 200
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HinfI GANTC 2 cut(s) 21, 393
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 427, 607
Hpy188I TCNGA 3 cut(s) 277, 542, 628
Hpy188III TCNNGA 2 cut(s) 18, 182
Hpy8I GTNNAC 2 cut(s) 427, 607
HpyAV CCTTC 3 cut(s) 94, 111, 470
HpyCH4III ACNGT 3 cut(s) 253, 271, 431
HpyCH4IV ACGT 2 cut(s) 499, 644
HpyCH4V TGCA 2 cut(s) 283, 497
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 311, 326, 524
HpyF3I CTNAG 3 cut(s) 171, 274, 384
HpySE526I ACGT 2 cut(s) 499, 644
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 2 cut(s) 185, 200
HspAI GCGC 1 cut(s) 193
KasI GGCGCC 1 cut(s) 192
KpnI GGTACC 1 cut(s) 351
Kzo9I GATC 1 cut(s) 124
LmnI GCTCC 2 cut(s) 103, 523
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 307
MaeI CTAG 1 cut(s) 203
MaeII ACGT 2 cut(s) 499, 644
MaeIII GTNAC 3 cut(s) 431, 500, 577
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 4 cut(s) 17, 73, 368, 611
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 385
MlsI TGGCCA 1 cut(s) 641
MluCI AATT 3 cut(s) 359, 439, 533
MluNI TGGCCA 1 cut(s) 641
Mly113I GGCGCC 1 cut(s) 193
MmeI TCCRAC 2 cut(s) 235, 545
MnlI CCTC 3 cut(s) 341, 544, 595
Mox20I TGGCCA 1 cut(s) 641
MscI TGGCCA 1 cut(s) 641
MslI CAYNNNNRTG 1 cut(s) 509
Msp20I TGGCCA 1 cut(s) 641
MspR9I CCNGG 4 cut(s) 336, 353, 379, 633
MunI CAATTG 1 cut(s) 533
MvaI CCWGG 4 cut(s) 336, 353, 379, 633
MwoI GCNNNNNNNGC 4 cut(s) 302, 311, 326, 524
NarI GGCGCC 1 cut(s) 193
NdeII GATC 1 cut(s) 124
NlaIII CATG 2 cut(s) 185, 200
NlaIV GGNNCC 3 cut(s) 194, 221, 349
NmeAIII GCCGAG 1 cut(s) 322
NmuCI GTSAC 1 cut(s) 500
PagI TCATGA 1 cut(s) 181
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PfeI GAWTC 2 cut(s) 21, 393
PflFI GACNNNGTC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 85
PluTI GGCGCC 1 cut(s) 196
PmaCI CACGTG 1 cut(s) 645
PmlI CACGTG 1 cut(s) 645
Ppu21I YACGTR 1 cut(s) 645
PpuMI RGGWCCY 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 219
Psp6I CCWGG 4 cut(s) 334, 351, 377, 631
PspCI CACGTG 1 cut(s) 645
PspGI CCWGG 4 cut(s) 334, 351, 377, 631
PspN4I GGNNCC 3 cut(s) 194, 221, 349
PspPI GGNCC 2 cut(s) 219, 304
PspPPI RGGWCCY 1 cut(s) 219
PsyI GACNNNGTC 1 cut(s) 13
RsaI GTAC 2 cut(s) 349, 561
RsaNI GTAC 2 cut(s) 348, 560
RseI CAYNNNNRTG 1 cut(s) 509
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 1 cut(s) 124
Sau96I GGNCC 2 cut(s) 219, 304
ScrFI CCNGG 4 cut(s) 336, 353, 379, 633
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 1 cut(s) 307
SfcI CTRYAG 1 cut(s) 231
SfoI GGCGCC 1 cut(s) 194
SinI GGWCC 1 cut(s) 219
SmiMI CAYNNNNRTG 1 cut(s) 509
Sse9I AATT 3 cut(s) 359, 439, 533
SsiI CCGC 2 cut(s) 189, 400
SspDI GGCGCC 1 cut(s) 192
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 4 cut(s) 334, 351, 377, 631
StyI CCWWGG 2 cut(s) 202, 215
TaaI ACNGT 3 cut(s) 253, 271, 431
TaiI ACGT 2 cut(s) 502, 647
TaqI TCGA 3 cut(s) 17, 90, 459
TasI AATT 3 cut(s) 359, 439, 533
TfiI GAWTC 2 cut(s) 21, 393
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 1 cut(s) 83
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 4 cut(s) 170, 445, 489, 588
Tth111I GACNNNGTC 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 219
XmaJI CCTAGG 1 cut(s) 202
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.