Rorug03G0149400
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011648
Physical Location & Seq
Forward (+)
12300982 .. 12304139
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0149400.1

Sequence Viewer

Length: 1635 bp
ATGGAAGCACCTGTGACTGTGAGGCAGCAAAGCTTCTGCGAGAAGAAGATGACAAAGCAGTTGACAGGGAAGCGCGAAGATGCCCCCTTGCATTTGGCTGCAAGAGCAGGAAATTTGGGATTGGTGTTAGAAATCATCTCAAATGGTGGGGATGCAGAGTTGAATGAAGTGTTAATGAAGCAGAATCACTCGGGTGAAACCGCCTTGTATGTGGCGGCTGAGTGTGGCTATGTTGATTTGGTGAAGGAAATGATCAAGTACTGTGATGTTGGTTTAGCTAGCATCAAAGCTAGAAATGGCTGTGATGCATTTCACATTGCTTCCAAGCAAGGTCACTTGGAGGTATTGAAGGTTTTCATGAAGGCAATACCAGAACTTTCAATGACTGTTGATCAAACAAACACCACAGCATTGCACGCCGCAGCAGCGCAGGGTCACATTGAAGTAGTAAATTTCCTTTTGGAGAATGGTTGCAGCTTGCTGACCATAGCAAGAAGCAATGCAAAAACCGCCTTTCATTCCGCAGCAAGAAATGGGCATTTAGATGTCGTCAAGGCACTCTTGAGAAAAGAACCTGAAATTGCAACGAGAAAGGATAAGAAGGGGCAGACAGCTCTCCATATGGCAGTTAAGGGACAGAATGTTGAGCTGGTGGATGAGTTCGCAAAAGCGGATCCTTCTATGATAAATATGATGGATAACAAGGGCAACACTGCCTTGCATATAGCAACCCGGAAGGGTCGAGCTCAGATTGTTCAGCAGCTGTTGGGTTATAAGGGAATTGCCACAAGTACCATTAACAACTCTGGTGAAACTGTTCTTGACACTGCACAGAAAAATGGGCAATCTGTGATTGCTGCCATTCTAGAAGAGAAAGGCATTCAAAGTGCCAAATCCATGAAACCACCAACCATAATCGCAAACCGAGAGCTCAAACAAACTGTCAGTGACATAAAACATGAGGTGCATGACCAGATCAAGCAAACACGACATACACGAAAGCAAGTACATGGTATGGTAAAACGGCTCAGCAAAATGCACTCAGATGGGCTTAACAACGCAATAAACTCCACCACTGTTGTGGCTGTCCTCATTGCCACCGTTGCCTTTGCTGCAATTTTCACTGTCCCAGGCCAATACCCCAATAATCTTCCACCTGGATACTCTCCTGGAGAAGCAAACATTGCCCCAAAAGCCGGGTTCATAGTCTTCTTCATCTTTGATTCTTTTGCACTCTTCATATCATTGGCTGTAGTGGTGGTTCAGACTTCTATTGTTGTTATAGAGAGGAAGCAAAAAGAGAAACTGATGACAGTTATAAACAAGCTGATGTGGATGGCTTGTGTGATGGTTTCGGTTGCATTTCTTGCACTGTCTTATGTAATCGTCGGAGAGAAAGAGAAATGGTTGGCTGTTGGGGTAACAGGTATAGGTACAGTGATTATGGGAATGACATTGGGAACAATGTGTTATTGGATGGTTGTGCAGAGAATTGAGGCTTCAAAACTTCGTAGCATCACTCGGAGATCATCAATGAGCACTAGGTCACACTCACATTCTGGGTCTTTCTCAGTGATGTCAGACTCTGAGCTTATCAACACTGAGTTTAAGAAGAAGAAAGTGTATGCAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

544

Amino Acids

59.36

Weight (kDa)

9.41

Isoelectric Point (pI)

35.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 20 - 90 1e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 98 - 160 6.1e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 138 - 188 3.7e-09 Ankyrin repeats (many copies)
Ank_2 PF12796 167 - 222 2.3e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 173 - 218 1.9e-08 Ankyrin repeats (many copies)
Ank_5 PF13857 192 - 243 4.9e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 215 - 294 6.9e-09 Ankyrin repeats (3 copies)
PGG PF13962 350 - 461 1.7e-28 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35990 AT2G35990 AT2G35990 AT5G06300
fragaria_vesca FvH4_6g20040 FvH4_6g20040 FvH4_6g20040
malus_domestica MD12G1037500.v1.1 MD14G1036500.v1.1
prunus_persica Prupe.7G097700_v2.0.a1 Prupe.7G097700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475501
rosa_laevigata RLG00000023840
rosa_multiflora Rmu_sc0000539.1_g000004
rosa_roxburghii Rroxscaffold_6G00406230
rosa_rugosa Rorug03G0149300 Rorug03G0149400
rosa_samantha Rh3BG229300
rosa_wichuraiana Rw3G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 774, 1319
AccII CGCG 1 cut(s) 75
AciI CCGC 6 cut(s) 201, 215, 420, 510, 522, 671
AclWI GGATC 2 cut(s) 668, 681
AcsI RAATTY 2 cut(s) 112, 451
AfaI GTAC 4 cut(s) 260, 793, 1008, 1435
AfiI CCNNNNNNNGG 1 cut(s) 1196
AgsI TTSAA 6 cut(s) 163, 349, 381, 443, 884, 1503
AjnI CCWGG 3 cut(s) 1129, 1156, 1168
AleI CACNNNNGTG 2 cut(s) 192, 1079
Alw21I GWGCWC 3 cut(s) 748, 933, 1541
AlwI GGATC 2 cut(s) 668, 681
AlwNI CAGNNNCTG 2 cut(s) 763, 1586
Ama87I CYCGRG 1 cut(s) 190
AoxI GGCC 1 cut(s) 1132
ApeKI GCWGC 9 cut(s) 25, 98, 422, 425, 474, 524, 760, 857, 1112
ApoI RAATTY 2 cut(s) 112, 451
ArsI GACNNNNNNTTYG 2 cut(s) 927, 959
Asp700I GAANNNNTTC 2 cut(s) 353, 816
AspLEI GCGC 2 cut(s) 75, 430
AsuC2I CCSGG 2 cut(s) 733, 1198
AsuHPI GGTGA 3 cut(s) 206, 253, 821
AsuNHI GCTAGC 1 cut(s) 278
AvaI CYCGRG 1 cut(s) 190
BamHI GGATCC 1 cut(s) 673
BanII GRGCYC 2 cut(s) 748, 933
BbsI GAAGAC 1 cut(s) 1201
Bbv12I GWGCWC 3 cut(s) 748, 933, 1541
BbvI GCAGC 9 cut(s) 37, 85, 434, 437, 486, 536, 772, 844, 1099
BccI CCATC 5 cut(s) 688, 1040, 1330, 1342, 1471
BceAI ACGGC 1 cut(s) 1040
BciT130I CCWGG 3 cut(s) 1131, 1158, 1170
BciVI GTATCC 1 cut(s) 1154
BclI TGATCA 2 cut(s) 252, 391
BcnI CCSGG 2 cut(s) 733, 1198
BfaI CTAG 4 cut(s) 279, 291, 866, 1542
BfmI CTRYAG 1 cut(s) 1251
BfuI GTATCC 1 cut(s) 1154
BlpI GCTNAGC 1 cut(s) 1028
BmcAI AGTACT 1 cut(s) 260
Bme1390I CCNGG 5 cut(s) 733, 1131, 1158, 1170, 1198
BmeT110I CYCGRG 1 cut(s) 190
BmiI GGNNCC 1 cut(s) 675
BmrFI CCNGG 5 cut(s) 733, 1131, 1158, 1170, 1198
BmsI GCATC 5 cut(s) 70, 142, 291, 295, 1524
BmtI GCTAGC 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 1201
BpmI CTGGAG 1 cut(s) 1191
Bpu1102I GCTNAGC 1 cut(s) 1028
BpuEI CTTGAG 1 cut(s) 583
BpuMI CCSGG 2 cut(s) 733, 1198
BsaJI CCNNGG 1 cut(s) 1129
Bsc4I CCNNNNNNNGG 1 cut(s) 1196
Bse3DI GCAATG 5 cut(s) 315, 410, 505, 1092, 1182
BseBI CCWGG 3 cut(s) 1131, 1158, 1170
BseDI CCNNGG 1 cut(s) 1129
BseGI GGATG 4 cut(s) 157, 661, 1341, 1482
BseLI CCNNNNNNNGG 1 cut(s) 1196
BseMI GCAATG 5 cut(s) 315, 410, 505, 1092, 1182
BseMII CTCAG 7 cut(s) 210, 761, 1042, 1056, 1578, 1584, 1593
BseXI GCAGC 9 cut(s) 37, 85, 434, 437, 486, 536, 772, 844, 1099
BsgI GTGCAG 2 cut(s) 813, 1505
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 1134
BsiHKAI GWGCWC 3 cut(s) 748, 933, 1541
BsiHKCI CYCGRG 1 cut(s) 190
BsiSI CCGG 2 cut(s) 733, 1197
BslFI GGGAC 2 cut(s) 648, 1112
BslI CCNNNNNNNGG 1 cut(s) 1196
BsmFI GGGAC 2 cut(s) 648, 1112
BsmI GAATGC 1 cut(s) 879
BsnI GGCC 1 cut(s) 1134
BsoBI CYCGRG 1 cut(s) 190
Bsp1286I GDGCHC 3 cut(s) 748, 933, 1541
Bsp143I GATC 5 cut(s) 252, 391, 673, 975, 1526
Bsp1720I GCTNAGC 1 cut(s) 1028
BspACI CCGC 6 cut(s) 201, 215, 420, 510, 522, 671
BspANI GGCC 1 cut(s) 1134
BspCNI CTCAG 7 cut(s) 211, 760, 1041, 1055, 1579, 1583, 1594
BspFNI CGCG 1 cut(s) 75
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 675
BspOI GCTAGC 1 cut(s) 282
BspPI GGATC 2 cut(s) 668, 681
BsrDI GCAATG 5 cut(s) 315, 410, 505, 1092, 1182
BssECI CCNNGG 1 cut(s) 1129
BssMI GATC 5 cut(s) 252, 391, 673, 975, 1526
Bst2UI CCWGG 3 cut(s) 1131, 1158, 1170
Bst6I CTCTTC 2 cut(s) 864, 1241
BstAPI GCANNNNNTGC 2 cut(s) 1184, 1367
BstC8I GCNNGC 3 cut(s) 280, 417, 479
BstDEI CTNAG 7 cut(s) 219, 747, 1028, 1042, 1570, 1587, 1602
BstF5I GGATG 4 cut(s) 157, 661, 1341, 1482
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 2 cut(s) 75, 430
BstKTI GATC 5 cut(s) 255, 394, 676, 978, 1529
BstMBI GATC 5 cut(s) 252, 391, 673, 975, 1526
BstMWI GCNNNNNNNGC 9 cut(s) 104, 416, 425, 509, 1103, 1112, 1184, 1193, 1367
BstNI CCWGG 3 cut(s) 1131, 1158, 1170
BstSCI CCNGG 5 cut(s) 731, 1129, 1156, 1168, 1196
BstSFI CTRYAG 1 cut(s) 1251
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 9 cut(s) 37, 85, 434, 437, 486, 536, 772, 844, 1099
BstV2I GAAGAC 1 cut(s) 1201
BstX2I RGATCY 1 cut(s) 673
BstXI CCANNNNNNTGG 1 cut(s) 1081
BstYI RGATCY 1 cut(s) 673
BsuI GTATCC 1 cut(s) 1154
BsuRI GGCC 1 cut(s) 1134
BtsCI GGATG 4 cut(s) 157, 661, 1341, 1482
BtsI GCAGTG 2 cut(s) 711, 825
BtsIMutI CAGTG 9 cut(s) 711, 825, 952, 1074, 1122, 1370, 1443, 1578, 1599
Cac8I GCNNGC 3 cut(s) 280, 417, 479
CaiI CAGNNNCTG 2 cut(s) 763, 1586
CciI TCATGA 1 cut(s) 357
CfoI GCGC 2 cut(s) 75, 430
Csp6I GTAC 4 cut(s) 259, 792, 1007, 1434
CspCI CAANNNNNGTGG 2 cut(s) 1060, 1095
CviAII CATG 5 cut(s) 358, 898, 959, 968, 1010
CviQI GTAC 4 cut(s) 259, 792, 1007, 1434
DdeI CTNAG 7 cut(s) 219, 747, 1028, 1042, 1570, 1587, 1602
DpnI GATC 5 cut(s) 254, 393, 675, 977, 1528
DpnII GATC 5 cut(s) 252, 391, 673, 975, 1526
Eam1104I CTCTTC 2 cut(s) 864, 1241
EarI CTCTTC 2 cut(s) 864, 1241
Ecl136II GAGCTC 2 cut(s) 746, 931
Eco24I GRGCYC 2 cut(s) 748, 933
Eco53kI GAGCTC 2 cut(s) 746, 931
Eco88I CYCGRG 1 cut(s) 190
EcoICRI GAGCTC 2 cut(s) 746, 931
EcoRII CCWGG 3 cut(s) 1129, 1156, 1168
EcoT22I ATGCAT 1 cut(s) 310
EcoT38I GRGCYC 2 cut(s) 748, 933
FaeI CATG 5 cut(s) 361, 901, 962, 971, 1013
FalI AAGNNNNNCTT 2 cut(s) 545, 577
FaqI GGGAC 2 cut(s) 648, 1112
FatI CATG 5 cut(s) 357, 897, 958, 967, 1009
FauNDI CATATG 1 cut(s) 621
FbaI TGATCA 2 cut(s) 252, 391
FokI GGATG 4 cut(s) 164, 668, 1348, 1489
FriOI GRGCYC 2 cut(s) 748, 933
FspBI CTAG 4 cut(s) 279, 291, 866, 1542
GlaI GCGC 2 cut(s) 74, 429
GsuI CTGGAG 1 cut(s) 1191
HaeIII GGCC 1 cut(s) 1134
HapII CCGG 2 cut(s) 733, 1197
HhaI GCGC 2 cut(s) 75, 430
Hin1II CATG 5 cut(s) 361, 901, 962, 971, 1013
Hin6I GCGC 2 cut(s) 73, 428
HinP1I GCGC 2 cut(s) 73, 428
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 31
HinfI GANTC 3 cut(s) 184, 1223, 1583
HpaII CCGG 2 cut(s) 733, 1197
HphI GGTGA 3 cut(s) 206, 253, 821
Hpy166II GTNNAC 1 cut(s) 63
Hpy188I TCNGA 7 cut(s) 750, 1045, 1266, 1391, 1524, 1582, 1588
Hpy188III TCNNGA 4 cut(s) 358, 562, 821, 866
Hpy8I GTNNAC 1 cut(s) 63
Hpy99I CGWCG 1 cut(s) 1391
HpyAV CCTTC 6 cut(s) 238, 343, 355, 595, 687, 730
HpyF10VI GCNNNNNNNGC 9 cut(s) 104, 416, 425, 509, 1103, 1112, 1184, 1193, 1367
HpyF3I CTNAG 7 cut(s) 219, 747, 1028, 1042, 1570, 1587, 1602
Hsp92II CATG 5 cut(s) 361, 901, 962, 971, 1013
HspAI GCGC 2 cut(s) 73, 428
Ksp22I TGATCA 2 cut(s) 252, 391
Kzo9I GATC 5 cut(s) 252, 391, 673, 975, 1526
Lsp1109I GCAGC 9 cut(s) 37, 85, 434, 437, 486, 536, 772, 844, 1099
LweI GCATC 5 cut(s) 70, 142, 291, 295, 1524
MaeI CTAG 4 cut(s) 279, 291, 866, 1542
MaeIII GTNAC 6 cut(s) 13, 332, 434, 947, 1420, 1545
MalI GATC 5 cut(s) 254, 393, 675, 977, 1528
MboI GATC 5 cut(s) 252, 391, 673, 975, 1526
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 3 cut(s) 748, 933, 1541
MluCI AATT 7 cut(s) 112, 451, 579, 780, 1116, 1491, 1629
MlyI GAGTC 1 cut(s) 1577
MmeI TCCRAC 1 cut(s) 1369
MnlI CCTC 6 cut(s) 15, 334, 955, 1100, 1281, 1489
Mph1103I ATGCAT 1 cut(s) 310
MroXI GAANNNNTTC 2 cut(s) 353, 816
MseI TTAA 5 cut(s) 173, 630, 798, 1053, 1608
MslI CAYNNNNRTG 4 cut(s) 192, 543, 1044, 1079
MspA1I CMGCKG 1 cut(s) 763
MspI CCGG 2 cut(s) 733, 1197
MspR9I CCNGG 5 cut(s) 733, 1131, 1158, 1170, 1198
Mva1269I GAATGC 1 cut(s) 879
MvaI CCWGG 3 cut(s) 1131, 1158, 1170
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 9 cut(s) 104, 416, 425, 509, 1103, 1112, 1184, 1193, 1367
NciI CCSGG 2 cut(s) 733, 1198
NdeI CATATG 1 cut(s) 621
NdeII GATC 5 cut(s) 252, 391, 673, 975, 1526
NheI GCTAGC 1 cut(s) 278
NlaIII CATG 5 cut(s) 361, 901, 962, 971, 1013
NlaIV GGNNCC 1 cut(s) 675
NmuCI GTSAC 5 cut(s) 13, 332, 434, 947, 1545
NsiI ATGCAT 1 cut(s) 310
OliI CACNNNNGTG 2 cut(s) 192, 1079
PagI TCATGA 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 994
PctI GAATGC 1 cut(s) 879
PdmI GAANNNNTTC 2 cut(s) 353, 816
PfeI GAWTC 2 cut(s) 184, 1223
PfoI TCCNGGA 1 cut(s) 1168
PleI GAGTC 1 cut(s) 1577
PpsI GAGTC 1 cut(s) 1577
PsiI TTATAA 2 cut(s) 774, 1319
Psp124BI GAGCTC 2 cut(s) 748, 933
Psp6I CCWGG 3 cut(s) 1129, 1156, 1168
PspGI CCWGG 3 cut(s) 1129, 1156, 1168
PspN4I GGNNCC 1 cut(s) 675
PsrI GAACNNNNNNTAC 2 cut(s) 1245, 1277
PstNI CAGNNNCTG 2 cut(s) 763, 1586
PsuI RGATCY 1 cut(s) 673
PvuII CAGCTG 1 cut(s) 763
RsaI GTAC 4 cut(s) 260, 793, 1008, 1435
RsaNI GTAC 4 cut(s) 259, 792, 1007, 1434
RseI CAYNNNNRTG 4 cut(s) 192, 543, 1044, 1079
SacI GAGCTC 2 cut(s) 748, 933
SaqAI TTAA 5 cut(s) 173, 630, 798, 1053, 1608
Sau3AI GATC 5 cut(s) 252, 391, 673, 975, 1526
ScaI AGTACT 1 cut(s) 260
SchI GAGTC 1 cut(s) 1577
ScrFI CCNGG 5 cut(s) 733, 1131, 1158, 1170, 1198
SduI GDGCHC 3 cut(s) 748, 933, 1541
SfaNI GCATC 5 cut(s) 70, 142, 291, 295, 1524
SfcI CTRYAG 1 cut(s) 1251
SmiMI CAYNNNNRTG 4 cut(s) 192, 543, 1044, 1079
SmlI CTYRAG 1 cut(s) 562
SmoI CTYRAG 1 cut(s) 562
Sse9I AATT 7 cut(s) 112, 451, 579, 780, 1116, 1491, 1629
SsiI CCGC 6 cut(s) 201, 215, 420, 510, 522, 671
SspMI CTAG 4 cut(s) 279, 291, 866, 1542
SstI GAGCTC 2 cut(s) 748, 933
StyD4I CCNGG 5 cut(s) 731, 1129, 1156, 1168, 1196
TaqI TCGA 1 cut(s) 742
TasI AATT 7 cut(s) 112, 451, 579, 780, 1116, 1491, 1629
TatI WGTACW 2 cut(s) 258, 1006
TauI GCSGC 2 cut(s) 218, 422
TfiI GAWTC 2 cut(s) 184, 1223
Tru1I TTAA 5 cut(s) 173, 630, 798, 1053, 1608
Tru9I TTAA 5 cut(s) 173, 630, 798, 1053, 1608
TscAI CASTG 9 cut(s) 718, 832, 952, 1081, 1129, 1377, 1443, 1578, 1606
TseFI GTSAC 5 cut(s) 13, 332, 434, 947, 1545
TseI GCWGC 9 cut(s) 25, 98, 422, 425, 474, 524, 760, 857, 1112
Tsp45I GTSAC 5 cut(s) 13, 332, 434, 947, 1545
TspDTI ATGAA 9 cut(s) 180, 191, 346, 374, 506, 914, 1192, 1204, 1228
TspRI CASTG 9 cut(s) 718, 832, 952, 1081, 1129, 1377, 1443, 1578, 1606
XapI RAATTY 2 cut(s) 112, 451
XbaI TCTAGA 1 cut(s) 865
XcmI CCANNNNNNNNNTGG 1 cut(s) 1078
XmnI GAANNNNTTC 2 cut(s) 353, 816
XspI CTAG 4 cut(s) 279, 291, 866, 1542
ZrmI AGTACT 1 cut(s) 260
Zsp2I ATGCAT 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.