FvH4_6g20040
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011648
Physical Location & Seq
Reverse (-)
13456123 .. 13460536
4414 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20040.t4

Sequence Viewer

Length: 648 bp
ATGGAAGAGACAAACTCGAGATTCAATAGGATTTGTGTTTTCTGTGGTAGCAGTTCAGGCAAGAAAGCTAGCTACCAAGAAGCTGCTGTTGAACTCGGCAAGGAGCTGGTGGAGAGAAGGATTAACTTGGTGTATGGTGGTGGAAGCGTGGGGCTGATGGGTCTCGTTTCTCAGGCAGTTCATGATGGCGGGCGCCATGTTATAGGAGTTATTCCAAGAACCCTAATGCCCAGAGAGATAACTGGTGAGACTGTTGGAGAAGTGAGAACTGTATCAAATATGCACCAAAGGAAAGCCGAGATGGCTCGTCAAGCCGATGCTTTCATTGCTCTCCCGGGTGGTTACGGCACCCTAGAAGAATTGCTTGAAGTCATTACCTGGGCTCAGCTTGGAATTCACCGCAAACCTGTGGGGCTTTTGAATGTGGACGGCTACTACAATTCATTGTTGAGTTTCATTGATCAGGCCGTTGATGAAGGCTTTATTTCTCCTACTGCACGTCAGATCATCGTGTCTGCTCCAACCGCCAAGCAATTGGTTAGACAACTAGAGGAATATGTTCCTGAGTATGATGAAGTAACATCCAACTTGGTCTGGGAAGAGGTGGACAGGATAAATTATGTGTCCGAATCAGGGGTCGCCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

216

Amino Acids

23.63

Weight (kDa)

5.1

Isoelectric Point (pI)

33.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lysine_decarbox PF03641 15 - 136 2.1e-44 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35990 AT2G35990 AT2G35990 AT5G06300
fragaria_vesca FvH4_6g20040 FvH4_6g20040 FvH4_6g20040
malus_domestica MD12G1037500.v1.1 MD14G1036500.v1.1
prunus_persica Prupe.7G097700_v2.0.a1 Prupe.7G097700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475501
rosa_laevigata RLG00000023840
rosa_multiflora Rmu_sc0000539.1_g000004
rosa_roxburghii Rroxscaffold_6G00406230
rosa_rugosa Rorug03G0149300 Rorug03G0149400
rosa_samantha Rh3BG229300
rosa_wichuraiana Rw3G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 192, 347
AciI CCGC 3 cut(s) 189, 400, 525
AcsI RAATTY 1 cut(s) 393
AcvI CACGTG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 193
AfiI CCNNNNNNNGG 1 cut(s) 633
AgsI TTSAA 4 cut(s) 25, 92, 368, 421
AjiI CACGTC 1 cut(s) 500
AjnI CCWGG 1 cut(s) 377
AluBI AGCT 5 cut(s) 68, 72, 83, 106, 388
AluI AGCT 5 cut(s) 68, 72, 83, 106, 388
Alw26I GTCTC 3 cut(s) 2, 167, 242
Ama87I CYCGRG 2 cut(s) 16, 334
AoxI GGCC 1 cut(s) 465
ApeKI GCWGC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 393
ArsI GACNNNNNNTTYG 1 cut(s) 621
Asp700I GAANNNNTTC 1 cut(s) 558
AspLEI GCGC 1 cut(s) 195
AsuC2I CCSGG 2 cut(s) 335, 336
AsuHPI GGTGA 2 cut(s) 257, 389
AsuNHI GCTAGC 1 cut(s) 68
AvaI CYCGRG 2 cut(s) 16, 334
BanI GGYRCC 2 cut(s) 192, 347
BanII GRGCYC 1 cut(s) 385
BbrPI CACGTG 1 cut(s) 645
BbvI GCAGC 1 cut(s) 70
BccI CCATC 3 cut(s) 151, 179, 295
BceAI ACGGC 3 cut(s) 361, 445, 452
BciT130I CCWGG 1 cut(s) 379
BclI TGATCA 1 cut(s) 460
BcnI CCSGG 2 cut(s) 335, 336
BcoDI GTCTC 3 cut(s) 2, 167, 242
BfaI CTAG 3 cut(s) 69, 353, 548
BfoI RGCGCY 1 cut(s) 196
BglI GCCNNNNNGGC 1 cut(s) 302
BisI GCNGC 1 cut(s) 84
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 3 cut(s) 335, 336, 379
BmeT110I CYCGRG 2 cut(s) 16, 334
BmgBI CACGTC 1 cut(s) 500
BmiI GGNNCC 2 cut(s) 194, 349
BmrFI CCNGG 3 cut(s) 335, 336, 379
BmsI GCATC 1 cut(s) 307
BmtI GCTAGC 1 cut(s) 72
BplI GAGNNNNNCTC 1 cut(s) 31
Bpu1102I GCTNAGC 1 cut(s) 384
BpuMI CCSGG 2 cut(s) 335, 336
BsaAI YACGTR 1 cut(s) 645
BsaHI GRCGYC 1 cut(s) 193
BsaI GGTCTC 1 cut(s) 167
BsaJI CCNNGG 2 cut(s) 334, 378
Bsc4I CCNNNNNNNGG 1 cut(s) 633
Bse1I ACTGG 1 cut(s) 247
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 2 cut(s) 334, 378
BseGI GGATG 1 cut(s) 581
BseLI CCNNNNNNNGG 1 cut(s) 633
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 3 cut(s) 185, 398, 555
BseNI ACTGG 1 cut(s) 247
BseXI GCAGC 1 cut(s) 70
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 1 cut(s) 467
BshNI GGYRCC 2 cut(s) 192, 347
BsiHKCI CYCGRG 2 cut(s) 16, 334
BsiSI CCGG 1 cut(s) 335
BslI CCNNNNNNNGG 1 cut(s) 633
BsmAI GTCTC 3 cut(s) 2, 167, 242
BsnI GGCC 1 cut(s) 467
Bso31I GGTCTC 1 cut(s) 167
BsoBI CYCGRG 2 cut(s) 16, 334
Bsp1286I GDGCHC 1 cut(s) 385
Bsp143I GATC 2 cut(s) 460, 504
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 3 cut(s) 189, 400, 525
BspANI GGCC 1 cut(s) 467
BspCNI CTCAG 3 cut(s) 184, 397, 556
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 2 cut(s) 194, 349
BspOI GCTAGC 1 cut(s) 72
BspT107I GGYRCC 2 cut(s) 192, 347
BspTNI GGTCTC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 2 cut(s) 334, 378
BssMI GATC 2 cut(s) 460, 504
BssNI GRCGYC 1 cut(s) 193
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 2 cut(s) 253, 271
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 193
BstBAI YACGTR 1 cut(s) 645
BstC8I GCNNGC 2 cut(s) 70, 191
BstDEI CTNAG 3 cut(s) 171, 384, 564
BstF5I GGATG 1 cut(s) 581
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 2 cut(s) 463, 507
BstMAI GTCTC 3 cut(s) 2, 167, 242
BstMBI GATC 2 cut(s) 460, 504
BstMWI GCNNNNNNNGC 5 cut(s) 57, 302, 311, 326, 524
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 3 cut(s) 333, 334, 377
BstV1I GCAGC 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 535
BsuRI GGCC 1 cut(s) 467
BtrI CACGTC 1 cut(s) 500
BtsCI GGATG 1 cut(s) 581
Cac8I GCNNGC 2 cut(s) 70, 191
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 195
Cfr9I CCCGGG 1 cut(s) 334
CviAII CATG 2 cut(s) 182, 197
DdeI CTNAG 3 cut(s) 171, 384, 564
DinI GGCGCC 1 cut(s) 194
DpnI GATC 2 cut(s) 462, 506
DpnII GATC 2 cut(s) 460, 504
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco24I GRGCYC 1 cut(s) 385
Eco31I GGTCTC 1 cut(s) 167
Eco72I CACGTG 1 cut(s) 645
Eco88I CYCGRG 2 cut(s) 16, 334
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 1 cut(s) 377
EcoT38I GRGCYC 1 cut(s) 385
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
FaeI CATG 2 cut(s) 185, 200
FaiI YATR 8 cut(s) 135, 183, 198, 203, 281, 558, 570, 621
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FatI CATG 2 cut(s) 181, 196
FauI CCCGC 1 cut(s) 182
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 1 cut(s) 568
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 3 cut(s) 69, 353, 548
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 1 cut(s) 467
HapII CCGG 1 cut(s) 335
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 2 cut(s) 185, 200
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HinfI GANTC 2 cut(s) 21, 629
HpaII CCGG 1 cut(s) 335
HphI GGTGA 2 cut(s) 257, 389
Hpy166II GTNNAC 2 cut(s) 427, 607
Hpy188I TCNGA 2 cut(s) 504, 628
Hpy188III TCNNGA 3 cut(s) 18, 182, 563
Hpy8I GTNNAC 2 cut(s) 427, 607
HpyAV CCTTC 2 cut(s) 111, 470
HpyCH4III ACNGT 2 cut(s) 253, 271
HpyCH4IV ACGT 2 cut(s) 499, 644
HpyCH4V TGCA 2 cut(s) 283, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 302, 311, 326, 524
HpyF3I CTNAG 3 cut(s) 171, 384, 564
HpySE526I ACGT 2 cut(s) 499, 644
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 2 cut(s) 185, 200
HspAI GCGC 1 cut(s) 193
KasI GGCGCC 1 cut(s) 192
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 2 cut(s) 460, 504
LmnI GCTCC 2 cut(s) 103, 523
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 307
MaeI CTAG 3 cut(s) 69, 353, 548
MaeII ACGT 2 cut(s) 499, 644
MaeIII GTNAC 2 cut(s) 341, 577
MalI GATC 2 cut(s) 462, 506
MboI GATC 2 cut(s) 460, 504
MboII GAAGA 3 cut(s) 17, 368, 611
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 5 cut(s) 359, 393, 439, 533, 616
Mly113I GGCGCC 1 cut(s) 193
MmeI TCCRAC 3 cut(s) 235, 545, 609
MnlI CCTC 2 cut(s) 544, 595
MroXI GAANNNNTTC 1 cut(s) 558
MseI TTAA 1 cut(s) 123
MspI CCGG 1 cut(s) 335
MspR9I CCNGG 3 cut(s) 335, 336, 379
MunI CAATTG 1 cut(s) 533
MvaI CCWGG 1 cut(s) 379
MwoI GCNNNNNNNGC 5 cut(s) 57, 302, 311, 326, 524
NarI GGCGCC 1 cut(s) 193
NciI CCSGG 2 cut(s) 335, 336
NdeII GATC 2 cut(s) 460, 504
NheI GCTAGC 1 cut(s) 68
NlaIII CATG 2 cut(s) 185, 200
NlaIV GGNNCC 2 cut(s) 194, 349
NmeAIII GCCGAG 2 cut(s) 75, 322
PaeR7I CTCGAG 1 cut(s) 16
PagI TCATGA 1 cut(s) 181
PdmI GAANNNNTTC 1 cut(s) 558
PfeI GAWTC 2 cut(s) 21, 629
PkrI GCNGC 1 cut(s) 85
PluTI GGCGCC 1 cut(s) 196
PmaCI CACGTG 1 cut(s) 645
PmlI CACGTG 1 cut(s) 645
Ppu21I YACGTR 1 cut(s) 645
Psp6I CCWGG 1 cut(s) 377
PspCI CACGTG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 2 cut(s) 194, 349
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 460, 504
ScrFI CCNGG 3 cut(s) 335, 336, 379
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 1 cut(s) 307
SfoI GGCGCC 1 cut(s) 194
Sfr274I CTCGAG 1 cut(s) 16
SlaI CTCGAG 1 cut(s) 16
SmaI CCCGGG 1 cut(s) 336
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 5 cut(s) 359, 393, 439, 533, 616
SsiI CCGC 3 cut(s) 189, 400, 525
SspDI GGCGCC 1 cut(s) 192
SspMI CTAG 3 cut(s) 69, 353, 548
StyD4I CCNGG 3 cut(s) 333, 334, 377
TaaI ACNGT 2 cut(s) 253, 271
TaiI ACGT 2 cut(s) 502, 647
TaqI TCGA 1 cut(s) 17
TasI AATT 5 cut(s) 359, 393, 439, 533, 616
TfiI GAWTC 2 cut(s) 21, 629
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 6 cut(s) 170, 313, 432, 445, 489, 588
TspMI CCCGGG 1 cut(s) 334
XapI RAATTY 1 cut(s) 393
XhoI CTCGAG 1 cut(s) 16
XmaI CCCGGG 1 cut(s) 334
XmnI GAANNNNTTC 1 cut(s) 558
XspI CTAG 3 cut(s) 69, 353, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.