Prupe.7G097700_v2.0.a1
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0011648
Physical Location & Seq
Forward (+)
12922636 .. 12927369
4734 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G097700.1

Sequence Viewer

Length: 657 bp
ATGGAAGAGACAAAGTCGAGATTCAATAGGATTTGTGTTTTTTGTGGTAGCAGTTCAGGGAAGAAAGCCAGCTACCAAGAAGCTGCTGTTGAACTTGGGAAGGAGCTGGTGGAGCGAAGGATTGATTTGGTCTATGGTGGTGGAAGCGTGGGATTGATGGGTCTTGTTTCTCAGGCAGTTCATGATGGCGGGCGCCATGTCCTAGGAGTTATTCCAAGGACCTTAATGCCTAGAGAGTTAACCGGTGAGACTGTTGGTGAAGTGAGAACTGTCTCAGATATGCACCAAAGGAAAGCTGAGATGGCCCGTCAAGCCGATGCTTTCATTGCCCTCCCTGGTGGTTATGGCACTCTAGAAGAATTGCTGGAAGTCATTACCTGGGCTCAGCTTGGAATCCACCGCAAACCTGTGGGCCTCTTGAATGTGGACGGCTTCTACAATTCTTTGTTGAGTTTCATTGATAAGGCCGTTGATGAAGGCTTTATTTCTACTACTGCACGTCAAATCATCGTGTCTGCTCCAACAGCCAAGCAATTGGTCAGACAACTTGAGGAGTATGTACCAGAGCAGGATGAAATAACATCAAAGCTGGTTTGGGAAGAGGTGGACAGAATAAATTATGTGTCTGAACCACGTGATGTGTTGGCTTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

219

Amino Acids

24.13

Weight (kDa)

5.15

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35990 AT2G35990 AT2G35990 AT5G06300
fragaria_vesca FvH4_6g20040 FvH4_6g20040 FvH4_6g20040
malus_domestica MD12G1037500.v1.1 MD14G1036500.v1.1
prunus_persica Prupe.7G097700_v2.0.a1 Prupe.7G097700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475501
rosa_laevigata RLG00000023840
rosa_multiflora Rmu_sc0000539.1_g000004
rosa_roxburghii Rroxscaffold_6G00406230
rosa_rugosa Rorug03G0149300 Rorug03G0149400
rosa_samantha Rh3BG229300
rosa_wichuraiana Rw3G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 192
AciI CCGC 2 cut(s) 189, 400
AcvI CACGTG 1 cut(s) 635
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 1 cut(s) 561
AgeI ACCGGT 1 cut(s) 242
AgsI TTSAA 3 cut(s) 25, 92, 421
AjiI CACGTC 1 cut(s) 500
AjnI CCWGG 2 cut(s) 334, 377
AluBI AGCT 6 cut(s) 72, 83, 106, 296, 388, 589
AluI AGCT 6 cut(s) 72, 83, 106, 296, 388, 589
Alw26I GTCTC 3 cut(s) 2, 242, 277
AoxI GGCC 3 cut(s) 303, 412, 465
ApeKI GCWGC 1 cut(s) 83
AsiGI ACCGGT 1 cut(s) 242
AspA2I CCTAGG 1 cut(s) 202
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 3 cut(s) 219, 304, 412
AsuHPI GGTGA 2 cut(s) 257, 269
AvaII GGWCC 1 cut(s) 219
AvrII CCTAGG 1 cut(s) 202
BanI GGYRCC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 385
BbrPI CACGTG 1 cut(s) 635
BbvI GCAGC 1 cut(s) 70
BccI CCATC 3 cut(s) 151, 179, 295
BceAI ACGGC 2 cut(s) 445, 452
BciT130I CCWGG 2 cut(s) 336, 379
BcoDI GTCTC 3 cut(s) 2, 242, 277
BfaI CTAG 3 cut(s) 203, 231, 353
BfoI RGCGCY 1 cut(s) 196
BisI GCNGC 1 cut(s) 84
BlnI CCTAGG 1 cut(s) 202
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 2 cut(s) 336, 379
Bme18I GGWCC 1 cut(s) 219
BmgBI CACGTC 1 cut(s) 500
BmgT120I GGNCC 3 cut(s) 219, 304, 412
BmiI GGNNCC 1 cut(s) 194
BmrFI CCNGG 2 cut(s) 336, 379
BmsI GCATC 1 cut(s) 307
Bpu1102I GCTNAGC 1 cut(s) 384
BpuEI CTTGAG 1 cut(s) 569
BsaAI YACGTR 1 cut(s) 635
BsaHI GRCGYC 1 cut(s) 193
BsaJI CCNNGG 4 cut(s) 202, 215, 334, 378
BsaWI WCCGGW 1 cut(s) 242
Bse118I RCCGGY 1 cut(s) 242
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 2 cut(s) 336, 379
BseDI CCNNGG 4 cut(s) 202, 215, 334, 378
BseGI GGATG 1 cut(s) 577
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 4 cut(s) 185, 288, 288, 398
BseRI GAGGAG 1 cut(s) 566
BseXI GCAGC 1 cut(s) 70
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 3 cut(s) 305, 414, 467
BshNI GGYRCC 1 cut(s) 192
BshTI ACCGGT 1 cut(s) 242
BsiSI CCGG 1 cut(s) 243
BsmAI GTCTC 3 cut(s) 2, 242, 277
BsnI GGCC 3 cut(s) 305, 414, 467
Bsp1286I GDGCHC 1 cut(s) 385
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 2 cut(s) 189, 400
BspANI GGCC 3 cut(s) 305, 414, 467
BspCNI CTCAG 4 cut(s) 184, 287, 289, 397
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 1 cut(s) 194
BspT107I GGYRCC 1 cut(s) 192
BsrDI GCAATG 1 cut(s) 324
BsrFI RCCGGY 1 cut(s) 242
BssAI RCCGGY 1 cut(s) 242
BssECI CCNNGG 4 cut(s) 202, 215, 334, 378
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 2 cut(s) 202, 215
Bst2UI CCWGG 2 cut(s) 336, 379
Bst4CI ACNGT 2 cut(s) 253, 271
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 193
BstBAI YACGTR 1 cut(s) 635
BstC8I GCNNGC 2 cut(s) 70, 191
BstDEI CTNAG 4 cut(s) 171, 274, 297, 384
BstF5I GGATG 1 cut(s) 577
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstMAI GTCTC 3 cut(s) 2, 242, 277
BstMWI GCNNNNNNNGC 5 cut(s) 112, 302, 311, 326, 524
BstNI CCWGG 2 cut(s) 336, 379
BstSCI CCNGG 2 cut(s) 334, 377
BstV1I GCAGC 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 535
BsuRI GGCC 3 cut(s) 305, 414, 467
BtrI CACGTC 1 cut(s) 500
BtsCI GGATG 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 70, 191
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 195
Cfr10I RCCGGY 1 cut(s) 242
Cfr13I GGNCC 3 cut(s) 219, 304, 412
Csp6I GTAC 1 cut(s) 560
CspAI ACCGGT 1 cut(s) 242
CviAII CATG 2 cut(s) 182, 197
CviQI GTAC 1 cut(s) 560
DdeI CTNAG 4 cut(s) 171, 274, 297, 384
DinI GGCGCC 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 202, 215
Eco24I GRGCYC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 219
Eco72I CACGTG 1 cut(s) 635
EcoO109I RGGNCCY 1 cut(s) 219
EcoRII CCWGG 2 cut(s) 334, 377
EcoT14I CCWWGG 2 cut(s) 202, 215
EcoT38I GRGCYC 1 cut(s) 385
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
ErhI CCWWGG 2 cut(s) 202, 215
FaeI CATG 2 cut(s) 185, 200
FaiI YATR 7 cut(s) 135, 183, 198, 281, 345, 558, 621
FatI CATG 2 cut(s) 181, 196
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 1 cut(s) 584
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 3 cut(s) 203, 231, 353
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 3 cut(s) 305, 414, 467
HapII CCGG 1 cut(s) 243
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 2 cut(s) 185, 200
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HincII GTYRAC 1 cut(s) 240
HindII GTYRAC 1 cut(s) 240
HinfI GANTC 2 cut(s) 21, 393
HpaI GTTAAC 1 cut(s) 240
HpaII CCGG 1 cut(s) 243
HphI GGTGA 2 cut(s) 257, 269
Hpy166II GTNNAC 3 cut(s) 240, 427, 607
Hpy188I TCNGA 3 cut(s) 277, 542, 628
Hpy188III TCNNGA 4 cut(s) 18, 182, 353, 418
Hpy8I GTNNAC 3 cut(s) 240, 427, 607
HpyAV CCTTC 3 cut(s) 94, 111, 470
HpyCH4III ACNGT 2 cut(s) 253, 271
HpyCH4IV ACGT 2 cut(s) 499, 634
HpyCH4V TGCA 2 cut(s) 283, 497
HpyF10VI GCNNNNNNNGC 5 cut(s) 112, 302, 311, 326, 524
HpyF3I CTNAG 4 cut(s) 171, 274, 297, 384
HpySE526I ACGT 2 cut(s) 499, 634
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 2 cut(s) 185, 200
HspAI GCGC 1 cut(s) 193
KasI GGCGCC 1 cut(s) 192
KspAI GTTAAC 1 cut(s) 240
LmnI GCTCC 3 cut(s) 103, 112, 523
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 307
MaeI CTAG 3 cut(s) 203, 231, 353
MaeII ACGT 2 cut(s) 499, 634
MboII GAAGA 4 cut(s) 17, 73, 368, 611
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 4 cut(s) 359, 439, 533, 616
Mly113I GGCGCC 1 cut(s) 193
MmeI TCCRAC 1 cut(s) 545
MnlI CCTC 4 cut(s) 341, 425, 544, 595
MseI TTAA 2 cut(s) 224, 239
MspI CCGG 1 cut(s) 243
MspR9I CCNGG 2 cut(s) 336, 379
MunI CAATTG 1 cut(s) 533
MvaI CCWGG 2 cut(s) 336, 379
MwoI GCNNNNNNNGC 5 cut(s) 112, 302, 311, 326, 524
NarI GGCGCC 1 cut(s) 193
NlaIII CATG 2 cut(s) 185, 200
NlaIV GGNNCC 1 cut(s) 194
PagI TCATGA 1 cut(s) 181
PfeI GAWTC 2 cut(s) 21, 393
PflFI GACNNNGTC 1 cut(s) 13
PinAI ACCGGT 1 cut(s) 242
PkrI GCNGC 1 cut(s) 85
PluTI GGCGCC 1 cut(s) 196
PmaCI CACGTG 1 cut(s) 635
PmlI CACGTG 1 cut(s) 635
Ppu21I YACGTR 1 cut(s) 635
PpuMI RGGWCCY 1 cut(s) 219
Psp5II RGGWCCY 1 cut(s) 219
Psp6I CCWGG 2 cut(s) 334, 377
PspCI CACGTG 1 cut(s) 635
PspGI CCWGG 2 cut(s) 334, 377
PspN4I GGNNCC 1 cut(s) 194
PspPI GGNCC 3 cut(s) 219, 304, 412
PspPPI RGGWCCY 1 cut(s) 219
PsyI GACNNNGTC 1 cut(s) 13
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
SaqAI TTAA 2 cut(s) 224, 239
SatI GCNGC 1 cut(s) 84
Sau96I GGNCC 3 cut(s) 219, 304, 412
ScrFI CCNGG 2 cut(s) 336, 379
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 1 cut(s) 307
SfoI GGCGCC 1 cut(s) 194
SinI GGWCC 1 cut(s) 219
SmlI CTYRAG 1 cut(s) 548
SmoI CTYRAG 1 cut(s) 548
Sse9I AATT 4 cut(s) 359, 439, 533, 616
SsiI CCGC 2 cut(s) 189, 400
SspDI GGCGCC 1 cut(s) 192
SspMI CTAG 3 cut(s) 203, 231, 353
StyD4I CCNGG 2 cut(s) 334, 377
StyI CCWWGG 2 cut(s) 202, 215
TaaI ACNGT 2 cut(s) 253, 271
TaiI ACGT 2 cut(s) 502, 637
TaqI TCGA 1 cut(s) 17
TasI AATT 4 cut(s) 359, 439, 533, 616
TfiI GAWTC 2 cut(s) 21, 393
Tru1I TTAA 2 cut(s) 224, 239
Tru9I TTAA 2 cut(s) 224, 239
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 5 cut(s) 170, 313, 445, 489, 588
Tth111I GACNNNGTC 1 cut(s) 13
VpaK11BI GGWCC 1 cut(s) 219
XbaI TCTAGA 1 cut(s) 352
XmaJI CCTAGG 1 cut(s) 202
XspI CTAG 3 cut(s) 203, 231, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.