MD12G1037500.v1.1
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011648
Physical Location & Seq
Reverse (-)
4010003 .. 4015080
5078 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1037500.v1.1.491

Sequence Viewer

Length: 648 bp
ATGGAAGAGACAAAGTCGAGATTCAATAGGATTTGTGTTTTTTGTGGTAGCAGTTCAGGGAAGAAGGCCAGCTACCAAGAAGCTGCTGTTGAACTTGGGAAGGAACTGGTGGAGAGAAGGATTGATCTGGTCTATGGTGGTGGAAGCGTGGGATTGATGGGTCTTGTTTCTCAGGCAGTTCATGATGGCGGGCGCCATGTCCTAGGTGTTATTCCGAGGACATTAATGCCTAGAGAGATAACTGGTGAAACTGTTGGAGAAGTGAGAACTGTCTCAGATATGCACCAAAGGAAAGCCGAGATGGCCCGTCAAGCTGATGCTTTCATTGCCCTCCCTGGTGGCTATGGTACCCTAGAAGAATTGCTGGAAGTCATTACCTGGGCTCAACTTGGAATCCACCGCAAACCTGTGGGGCTTTTGAATGTGGACGGTTACTACAATTCGTTGTTGTCTTTTATTGATAAGGCCGTTGATGAAGGCTTTATTTCTCCTACTGCACGGCACATCATCGTGTCTGCTCCAACTGCAAAGCAATTGGTCAGACAACTGGAGGAATATGTACCAGAGCACGATGAAATAACATCAAAGTTGGTGTGGGAAGAGGTGGACAGAAAAAGTTATGTGTCTGAACCAGGAGTAGCAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

216

Amino Acids

23.66

Weight (kDa)

5.58

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 16 - 141 2.6e-14 SLOG cluster4 family
Lysine_decarbox PF03641 53 - 183 3.4e-50 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35990 AT2G35990 AT2G35990 AT5G06300
fragaria_vesca FvH4_6g20040 FvH4_6g20040 FvH4_6g20040
malus_domestica MD12G1037500.v1.1 MD14G1036500.v1.1
prunus_persica Prupe.7G097700_v2.0.a1 Prupe.7G097700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475501
rosa_laevigata RLG00000023840
rosa_multiflora Rmu_sc0000539.1_g000004
rosa_roxburghii Rroxscaffold_6G00406230
rosa_rugosa Rorug03G0149300 Rorug03G0149400
rosa_samantha Rh3BG229300
rosa_wichuraiana Rw3G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 347
AccB1I GGYRCC 2 cut(s) 192, 347
AciI CCGC 2 cut(s) 189, 400
AcyI GRCGYC 1 cut(s) 193
AfaI GTAC 2 cut(s) 349, 561
AgsI TTSAA 3 cut(s) 25, 92, 421
AjnI CCWGG 3 cut(s) 334, 377, 631
AluBI AGCT 3 cut(s) 72, 83, 314
AluI AGCT 3 cut(s) 72, 83, 314
Alw21I GWGCWC 1 cut(s) 570
Alw26I GTCTC 2 cut(s) 2, 277
AoxI GGCC 3 cut(s) 66, 303, 465
ApeKI GCWGC 1 cut(s) 83
AseI ATTAAT 1 cut(s) 224
Asp718I GGTACC 1 cut(s) 347
AspA2I CCTAGG 1 cut(s) 202
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 304
AsuHPI GGTGA 1 cut(s) 257
AvrII CCTAGG 1 cut(s) 202
BanI GGYRCC 2 cut(s) 192, 347
BanII GRGCYC 1 cut(s) 385
BarI GAAGNNNNNNTAC 2 cut(s) 56, 88
Bbv12I GWGCWC 1 cut(s) 570
BbvI GCAGC 1 cut(s) 70
BccI CCATC 3 cut(s) 151, 179, 295
BceAI ACGGC 2 cut(s) 452, 515
BciT130I CCWGG 3 cut(s) 336, 379, 633
BcoDI GTCTC 2 cut(s) 2, 277
BfaI CTAG 3 cut(s) 203, 231, 353
BfoI RGCGCY 1 cut(s) 196
BglI GCCNNNNNGGC 1 cut(s) 302
BisI GCNGC 1 cut(s) 84
BlnI CCTAGG 1 cut(s) 202
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 3 cut(s) 336, 379, 633
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 2 cut(s) 194, 349
BmrFI CCNGG 3 cut(s) 336, 379, 633
BmsI GCATC 1 cut(s) 307
BpmI CTGGAG 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 193
BsaJI CCNNGG 4 cut(s) 202, 215, 334, 378
Bse1I ACTGG 3 cut(s) 111, 247, 552
Bse3DI GCAATG 1 cut(s) 324
BseBI CCWGG 3 cut(s) 336, 379, 633
BseDI CCNNGG 4 cut(s) 202, 215, 334, 378
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 2 cut(s) 185, 288
BseNI ACTGG 3 cut(s) 111, 247, 552
BseXI GCAGC 1 cut(s) 70
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 3 cut(s) 68, 305, 467
BshNI GGYRCC 2 cut(s) 192, 347
BsiHKAI GWGCWC 1 cut(s) 570
BsmAI GTCTC 2 cut(s) 2, 277
BsnI GGCC 3 cut(s) 68, 305, 467
Bsp1286I GDGCHC 2 cut(s) 385, 570
Bsp143I GATC 1 cut(s) 124
BspACI CCGC 2 cut(s) 189, 400
BspANI GGCC 3 cut(s) 68, 305, 467
BspCNI CTCAG 2 cut(s) 184, 287
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 2 cut(s) 194, 349
BspT107I GGYRCC 2 cut(s) 192, 347
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 3 cut(s) 111, 247, 552
BssECI CCNNGG 4 cut(s) 202, 215, 334, 378
BssMI GATC 1 cut(s) 124
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 1 cut(s) 202
Bst2UI CCWGG 3 cut(s) 336, 379, 633
Bst4CI ACNGT 3 cut(s) 253, 271, 431
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 193
BstC8I GCNNGC 2 cut(s) 70, 191
BstDEI CTNAG 2 cut(s) 171, 274
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 1 cut(s) 127
BstMAI GTCTC 2 cut(s) 2, 277
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 4 cut(s) 302, 311, 326, 524
BstNI CCWGG 3 cut(s) 336, 379, 633
BstSCI CCNGG 3 cut(s) 334, 377, 631
BstV1I GCAGC 1 cut(s) 70
BsuRI GGCC 3 cut(s) 68, 305, 467
Cac8I GCNNGC 2 cut(s) 70, 191
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 304
Csp6I GTAC 2 cut(s) 348, 560
CviAII CATG 2 cut(s) 182, 197
CviQI GTAC 2 cut(s) 348, 560
DdeI CTNAG 2 cut(s) 171, 274
DinI GGCGCC 1 cut(s) 194
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 202
Eco24I GRGCYC 1 cut(s) 385
EcoRII CCWGG 3 cut(s) 334, 377, 631
EcoT14I CCWWGG 1 cut(s) 202
EcoT38I GRGCYC 1 cut(s) 385
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 202
FaeI CATG 2 cut(s) 185, 200
FaiI YATR 7 cut(s) 135, 183, 198, 281, 345, 558, 621
FatI CATG 2 cut(s) 181, 196
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 1 cut(s) 84
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 3 cut(s) 203, 231, 353
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 569
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 3 cut(s) 68, 305, 467
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 2 cut(s) 185, 200
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HinfI GANTC 2 cut(s) 21, 393
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 427, 607
Hpy188I TCNGA 4 cut(s) 216, 277, 542, 628
Hpy188III TCNNGA 2 cut(s) 18, 182
Hpy8I GTNNAC 2 cut(s) 427, 607
HpyAV CCTTC 4 cut(s) 58, 94, 111, 470
HpyCH4III ACNGT 3 cut(s) 253, 271, 431
HpyCH4IV ACGT 1 cut(s) 644
HpyCH4V TGCA 3 cut(s) 283, 497, 527
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 311, 326, 524
HpyF3I CTNAG 2 cut(s) 171, 274
HpySE526I ACGT 1 cut(s) 644
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 2 cut(s) 185, 200
HspAI GCGC 1 cut(s) 193
KasI GGCGCC 1 cut(s) 192
KpnI GGTACC 1 cut(s) 351
Kzo9I GATC 1 cut(s) 124
LmnI GCTCC 1 cut(s) 523
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 307
MaeI CTAG 3 cut(s) 203, 231, 353
MaeII ACGT 1 cut(s) 644
MaeIII GTNAC 1 cut(s) 431
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 4 cut(s) 17, 73, 368, 611
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 2 cut(s) 385, 570
MluCI AATT 3 cut(s) 359, 439, 533
Mly113I GGCGCC 1 cut(s) 193
MmeI TCCRAC 2 cut(s) 235, 545
MnlI CCTC 4 cut(s) 210, 341, 544, 595
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 509
MspR9I CCNGG 3 cut(s) 336, 379, 633
MunI CAATTG 1 cut(s) 533
MvaI CCWGG 3 cut(s) 336, 379, 633
MwoI GCNNNNNNNGC 4 cut(s) 302, 311, 326, 524
NarI GGCGCC 1 cut(s) 193
NdeII GATC 1 cut(s) 124
NlaIII CATG 2 cut(s) 185, 200
NlaIV GGNNCC 2 cut(s) 194, 349
NmeAIII GCCGAG 1 cut(s) 322
PagI TCATGA 1 cut(s) 181
PfeI GAWTC 2 cut(s) 21, 393
PflFI GACNNNGTC 1 cut(s) 13
PkrI GCNGC 1 cut(s) 85
PluTI GGCGCC 1 cut(s) 196
PshBI ATTAAT 1 cut(s) 224
Psp6I CCWGG 3 cut(s) 334, 377, 631
PspGI CCWGG 3 cut(s) 334, 377, 631
PspN4I GGNNCC 2 cut(s) 194, 349
PspPI GGNCC 1 cut(s) 304
PsyI GACNNNGTC 1 cut(s) 13
RsaI GTAC 2 cut(s) 349, 561
RsaNI GTAC 2 cut(s) 348, 560
RseI CAYNNNNRTG 1 cut(s) 509
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 1 cut(s) 124
Sau96I GGNCC 1 cut(s) 304
ScrFI CCNGG 3 cut(s) 336, 379, 633
SduI GDGCHC 2 cut(s) 385, 570
SetI ASST 8 cut(s) 74, 85, 208, 316, 380, 409, 606, 647
SfaNI GCATC 1 cut(s) 307
SfoI GGCGCC 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 509
Sse9I AATT 3 cut(s) 359, 439, 533
SsiI CCGC 2 cut(s) 189, 400
SspDI GGCGCC 1 cut(s) 192
SspMI CTAG 3 cut(s) 203, 231, 353
StyD4I CCNGG 3 cut(s) 334, 377, 631
StyI CCWWGG 1 cut(s) 202
TaaI ACNGT 3 cut(s) 253, 271, 431
TaiI ACGT 1 cut(s) 647
TaqI TCGA 1 cut(s) 17
TasI AATT 3 cut(s) 359, 439, 533
TfiI GAWTC 2 cut(s) 21, 393
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 4 cut(s) 170, 313, 489, 588
Tth111I GACNNNGTC 1 cut(s) 13
VspI ATTAAT 1 cut(s) 224
XmaJI CCTAGG 1 cut(s) 202
XspI CTAG 3 cut(s) 203, 231, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.