Rroxscaffold_6G00406230
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0011648
Physical Location & Seq
Reverse (-)
28715361 .. 28719483
4123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00406230.1

Sequence Viewer

Length: 648 bp
ATGGAAGAGACAAACTCGAGATTCAATAGGATTTGTGTTTTCTGTGGTAGCAGTTCAGGCAAGAAAGCTAGCTACCAAGAAGCTGCTGTTGAACTCGGCAAGGAGCTGGTGGAGAGAAGGATTAATTTGGTGTATGGTGGTGGAAGCGTGGGATTGATGGGTCTTGTTTCTCAGGCAGTTCATGATGGCGGGCGCCATGTCCTAGGGGTTATTCCAAGGACCCTAATGCCCAGAGAGATAACTGGTGAGACTGTTGGAGAAGTGAGAACTGTATCAGGTATGCACCAAAGGAAAGCAGAGATGGCTCGTCAAGCCGATGCTTTCATTGCTCTCCCGGGCGGTTATGGCACCCTAGAAGAATTGCTTGAAGTCATTACCTGGGCTCAGCTTGGAATCCACCGCAAACCTGTGGGGCTTTTGAATGTGGACGGCTACTACAATTCCTTGTTGAGTTTTATTGATAAGGCCGTCGATGAAGGCTTTATTTCTCCTACTGCACGTCATATCATCGTGTCAGCTCCAACCGCCAAGCAATTGGTTATACAACTAGAGGAATATGTTCCGGAGTATGATGAAGTAACGTCCAAGTTGGTGTGGGAAGAGGTGGACAGGATAAATTACGTGTCTGAGTCGGGGGTCGCCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

215

Amino Acids

23.55

Weight (kDa)

5.31

Isoelectric Point (pI)

34.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LDcluster4 PF18306 16 - 141 3.2e-15 SLOG cluster4 family
Lysine_decarbox PF03641 53 - 182 3.7e-49 Possible lysine decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35990 AT2G35990 AT2G35990 AT5G06300
fragaria_vesca FvH4_6g20040 FvH4_6g20040 FvH4_6g20040
malus_domestica MD12G1037500.v1.1 MD14G1036500.v1.1
prunus_persica Prupe.7G097700_v2.0.a1 Prupe.7G097700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475501
rosa_laevigata RLG00000023840
rosa_multiflora Rmu_sc0000539.1_g000004
rosa_roxburghii Rroxscaffold_6G00406230
rosa_rugosa Rorug03G0149300 Rorug03G0149400
rosa_samantha Rh3BG229300
rosa_wichuraiana Rw3G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 192, 347
AccIII TCCGGA 1 cut(s) 562
AciI CCGC 4 cut(s) 189, 339, 400, 525
AcvI CACGTG 1 cut(s) 645
AcyI GRCGYC 1 cut(s) 193
AflIII ACRYGT 1 cut(s) 621
AgsI TTSAA 4 cut(s) 25, 92, 368, 421
AjiI CACGTC 1 cut(s) 500
AjnI CCWGG 1 cut(s) 377
AluBI AGCT 6 cut(s) 68, 72, 83, 106, 388, 518
AluI AGCT 6 cut(s) 68, 72, 83, 106, 388, 518
Alw26I GTCTC 2 cut(s) 2, 242
Ama87I CYCGRG 2 cut(s) 16, 334
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 1 cut(s) 465
ApeKI GCWGC 1 cut(s) 83
AseI ATTAAT 1 cut(s) 123
Asp700I GAANNNNTTC 1 cut(s) 558
AspA2I CCTAGG 1 cut(s) 202
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 219
AsuC2I CCSGG 2 cut(s) 335, 336
AsuHPI GGTGA 1 cut(s) 257
AsuNHI GCTAGC 1 cut(s) 68
AvaI CYCGRG 2 cut(s) 16, 334
AvaII GGWCC 1 cut(s) 219
AvrII CCTAGG 1 cut(s) 202
BanI GGYRCC 2 cut(s) 192, 347
BanII GRGCYC 1 cut(s) 385
BbrPI CACGTG 1 cut(s) 645
BbvI GCAGC 1 cut(s) 70
BccI CCATC 3 cut(s) 151, 179, 295
BceAI ACGGC 2 cut(s) 445, 452
BciT130I CCWGG 1 cut(s) 379
BcnI CCSGG 2 cut(s) 335, 336
BcoDI GTCTC 2 cut(s) 2, 242
BfaI CTAG 4 cut(s) 69, 203, 353, 548
BfoI RGCGCY 1 cut(s) 196
BisI GCNGC 1 cut(s) 84
BlnI CCTAGG 1 cut(s) 202
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 1 cut(s) 85
Bme1390I CCNGG 3 cut(s) 335, 336, 379
Bme18I GGWCC 1 cut(s) 219
BmeT110I CYCGRG 2 cut(s) 16, 334
BmgBI CACGTC 1 cut(s) 500
BmgT120I GGNCC 1 cut(s) 219
BmiI GGNNCC 3 cut(s) 194, 221, 349
BmrFI CCNGG 3 cut(s) 335, 336, 379
BmsI GCATC 1 cut(s) 307
BmtI GCTAGC 1 cut(s) 72
BplI GAGNNNNNCTC 1 cut(s) 31
Bpu1102I GCTNAGC 1 cut(s) 384
BpuMI CCSGG 2 cut(s) 335, 336
BsaAI YACGTR 2 cut(s) 622, 645
BsaHI GRCGYC 1 cut(s) 193
BsaJI CCNNGG 4 cut(s) 202, 215, 334, 378
BsaWI WCCGGW 1 cut(s) 562
Bse1I ACTGG 1 cut(s) 247
Bse3DI GCAATG 1 cut(s) 324
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 4 cut(s) 202, 215, 334, 378
BseMI GCAATG 1 cut(s) 324
BseMII CTCAG 3 cut(s) 185, 398, 618
BseNI ACTGG 1 cut(s) 247
BseXI GCAGC 1 cut(s) 70
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 1 cut(s) 467
BshNI GGYRCC 2 cut(s) 192, 347
BsiHKCI CYCGRG 2 cut(s) 16, 334
BsiSI CCGG 2 cut(s) 335, 563
BsmAI GTCTC 2 cut(s) 2, 242
BsnI GGCC 1 cut(s) 467
BsoBI CYCGRG 2 cut(s) 16, 334
Bsp1286I GDGCHC 1 cut(s) 385
Bsp13I TCCGGA 1 cut(s) 562
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 4 cut(s) 189, 339, 400, 525
BspANI GGCC 1 cut(s) 467
BspCNI CTCAG 3 cut(s) 184, 397, 619
BspEI TCCGGA 1 cut(s) 562
BspHI TCATGA 1 cut(s) 181
BspLI GGNNCC 3 cut(s) 194, 221, 349
BspOI GCTAGC 1 cut(s) 72
BspT107I GGYRCC 2 cut(s) 192, 347
BsrDI GCAATG 1 cut(s) 324
BsrI ACTGG 1 cut(s) 247
BssECI CCNNGG 4 cut(s) 202, 215, 334, 378
BssNI GRCGYC 1 cut(s) 193
BssT1I CCWWGG 2 cut(s) 202, 215
Bst2UI CCWGG 1 cut(s) 379
Bst4CI ACNGT 2 cut(s) 253, 271
Bst6I CTCTTC 1 cut(s) 594
BstACI GRCGYC 1 cut(s) 193
BstBAI YACGTR 2 cut(s) 622, 645
BstC8I GCNNGC 2 cut(s) 70, 191
BstDEI CTNAG 3 cut(s) 171, 384, 627
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstMAI GTCTC 2 cut(s) 2, 242
BstMWI GCNNNNNNNGC 6 cut(s) 57, 302, 311, 326, 345, 524
BstNI CCWGG 1 cut(s) 379
BstSCI CCNGG 3 cut(s) 333, 334, 377
BstV1I GCAGC 1 cut(s) 70
BstXI CCANNNNNNTGG 1 cut(s) 535
BsuRI GGCC 1 cut(s) 467
BtrI CACGTC 1 cut(s) 500
Cac8I GCNNGC 2 cut(s) 70, 191
CciI TCATGA 1 cut(s) 181
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 219
Cfr9I CCCGGG 1 cut(s) 334
CviAII CATG 2 cut(s) 182, 197
DdeI CTNAG 3 cut(s) 171, 384, 627
DinI GGCGCC 1 cut(s) 194
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
Eco130I CCWWGG 2 cut(s) 202, 215
Eco24I GRGCYC 1 cut(s) 385
Eco47I GGWCC 1 cut(s) 219
Eco72I CACGTG 1 cut(s) 645
Eco88I CYCGRG 2 cut(s) 16, 334
EcoO109I RGGNCCY 1 cut(s) 219
EcoRII CCWGG 1 cut(s) 377
EcoT14I CCWWGG 2 cut(s) 202, 215
EcoT38I GRGCYC 1 cut(s) 385
EgeI GGCGCC 1 cut(s) 194
EheI GGCGCC 1 cut(s) 194
ErhI CCWWGG 2 cut(s) 202, 215
FaeI CATG 2 cut(s) 185, 200
FaiI YATR 9 cut(s) 135, 183, 198, 281, 345, 504, 542, 558, 570
FalI AAGNNNNNCTT 2 cut(s) 348, 380
FatI CATG 2 cut(s) 181, 196
FauI CCCGC 1 cut(s) 182
Fnu4HI GCNGC 1 cut(s) 84
FriOI GRGCYC 1 cut(s) 385
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 4 cut(s) 69, 203, 353, 548
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 84
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 1 cut(s) 467
HapII CCGG 2 cut(s) 335, 563
HhaI GCGC 1 cut(s) 195
Hin1I GRCGYC 1 cut(s) 193
Hin1II CATG 2 cut(s) 185, 200
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HinfI GANTC 3 cut(s) 21, 393, 629
HpaII CCGG 2 cut(s) 335, 563
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 2 cut(s) 427, 607
Hpy188I TCNGA 1 cut(s) 628
Hpy188III TCNNGA 3 cut(s) 18, 182, 563
Hpy8I GTNNAC 2 cut(s) 427, 607
Hpy99I CGWCG 1 cut(s) 473
HpyAV CCTTC 2 cut(s) 111, 470
HpyCH4III ACNGT 2 cut(s) 253, 271
HpyCH4IV ACGT 4 cut(s) 499, 581, 621, 644
HpyCH4V TGCA 2 cut(s) 283, 497
HpyF10VI GCNNNNNNNGC 6 cut(s) 57, 302, 311, 326, 345, 524
HpyF3I CTNAG 3 cut(s) 171, 384, 627
HpySE526I ACGT 4 cut(s) 499, 581, 621, 644
Hsp92I GRCGYC 1 cut(s) 193
Hsp92II CATG 2 cut(s) 185, 200
HspAI GCGC 1 cut(s) 193
KasI GGCGCC 1 cut(s) 192
Kpn2I TCCGGA 1 cut(s) 562
LmnI GCTCC 2 cut(s) 103, 523
Lsp1109I GCAGC 1 cut(s) 70
LweI GCATC 1 cut(s) 307
MaeI CTAG 4 cut(s) 69, 203, 353, 548
MaeII ACGT 4 cut(s) 499, 581, 621, 644
MaeIII GTNAC 1 cut(s) 577
MboII GAAGA 3 cut(s) 17, 368, 611
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 1 cut(s) 385
MluCI AATT 5 cut(s) 124, 359, 439, 533, 616
Mly113I GGCGCC 1 cut(s) 193
MlyI GAGTC 1 cut(s) 638
MmeI TCCRAC 2 cut(s) 235, 545
MnlI CCTC 2 cut(s) 544, 595
MroI TCCGGA 1 cut(s) 562
MroXI GAANNNNTTC 1 cut(s) 558
MseI TTAA 1 cut(s) 123
MspI CCGG 2 cut(s) 335, 563
MspR9I CCNGG 3 cut(s) 335, 336, 379
MunI CAATTG 1 cut(s) 533
MvaI CCWGG 1 cut(s) 379
MwoI GCNNNNNNNGC 6 cut(s) 57, 302, 311, 326, 345, 524
NarI GGCGCC 1 cut(s) 193
NciI CCSGG 2 cut(s) 335, 336
NheI GCTAGC 1 cut(s) 68
NlaIII CATG 2 cut(s) 185, 200
NlaIV GGNNCC 3 cut(s) 194, 221, 349
NmeAIII GCCGAG 1 cut(s) 75
PaeR7I CTCGAG 1 cut(s) 16
PagI TCATGA 1 cut(s) 181
PdmI GAANNNNTTC 1 cut(s) 558
PfeI GAWTC 2 cut(s) 21, 393
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 1 cut(s) 637
PluTI GGCGCC 1 cut(s) 196
PmaCI CACGTG 1 cut(s) 645
PmlI CACGTG 1 cut(s) 645
PpsI GAGTC 1 cut(s) 637
Ppu21I YACGTR 2 cut(s) 622, 645
PpuMI RGGWCCY 1 cut(s) 219
PshBI ATTAAT 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 219
Psp6I CCWGG 1 cut(s) 377
PspCI CACGTG 1 cut(s) 645
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 3 cut(s) 194, 221, 349
PspPI GGNCC 1 cut(s) 219
PspPPI RGGWCCY 1 cut(s) 219
SaqAI TTAA 1 cut(s) 123
SatI GCNGC 1 cut(s) 84
Sau96I GGNCC 1 cut(s) 219
SchI GAGTC 1 cut(s) 638
ScrFI CCNGG 3 cut(s) 335, 336, 379
SduI GDGCHC 1 cut(s) 385
SfaNI GCATC 1 cut(s) 307
SfoI GGCGCC 1 cut(s) 194
Sfr274I CTCGAG 1 cut(s) 16
SinI GGWCC 1 cut(s) 219
SlaI CTCGAG 1 cut(s) 16
SmaI CCCGGG 1 cut(s) 336
SmlI CTYRAG 1 cut(s) 16
SmoI CTYRAG 1 cut(s) 16
Sse9I AATT 5 cut(s) 124, 359, 439, 533, 616
SsiI CCGC 4 cut(s) 189, 339, 400, 525
SspDI GGCGCC 1 cut(s) 192
SspMI CTAG 4 cut(s) 69, 203, 353, 548
StyD4I CCNGG 3 cut(s) 333, 334, 377
StyI CCWWGG 2 cut(s) 202, 215
TaaI ACNGT 2 cut(s) 253, 271
TaiI ACGT 4 cut(s) 502, 584, 624, 647
TaqI TCGA 2 cut(s) 17, 471
TasI AATT 5 cut(s) 124, 359, 439, 533, 616
TfiI GAWTC 2 cut(s) 21, 393
Tru1I TTAA 1 cut(s) 123
Tru9I TTAA 1 cut(s) 123
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 4 cut(s) 170, 313, 489, 588
TspMI CCCGGG 1 cut(s) 334
VpaK11BI GGWCC 1 cut(s) 219
VspI ATTAAT 1 cut(s) 123
XhoI CTCGAG 1 cut(s) 16
XmaI CCCGGG 1 cut(s) 334
XmaJI CCTAGG 1 cut(s) 202
XmnI GAANNNNTTC 1 cut(s) 558
XspI CTAG 4 cut(s) 69, 203, 353, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.