Rorug03G0149300
NAC Family

Cytokinin-activating enzyme working in the direct activation pathway. Phosphoribohydrolase that converts inactive cytokinin nucleotides to the biologically active free-base forms

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011648
Physical Location & Seq
Forward (+)
12295533 .. 12298895
3363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0149300.1

Sequence Viewer

Length: 1857 bp
ATGCTGAATGTCCGTTTGAGAACGGGACAAGATGCAGGAGGTGAGAGGGACCTGGAGAAGGGGCTGCTGGAGCCCTCAACGCCGGGAACTCGATCGGAGCCGCCGATGTCACCGCACACATCGAGGCTGGCGCCGTTTTTGTCCAATTCCAGCAAGTCTCTGCTGGTTTCGAATTCTAGCAAGTCTCTGCTGGTTTCGAATTCCGGCAAGTCTCTGATGGTTTCCAATTCCAGCAAGTCTCTGGTTATGTCGAATTCCGGCAACCGCTTGGATCAGAAGAAGAAGTACGTGCGGCAGGTGACCGGCCGCTTCAATGACACGGAGCTCCACCTGGCGGCGAAGCGGGGCGACGTCGCCGCCGTGAAGCATATCATCGGCGAGATTGATGCACAGATGATGGGGACCACGAGTGGGGCTGAGTTTGATGCGGAGGTGGCTGAGATTAGGTCTGCGGTTGTGAATGAGGTCAATGAGTTGGGAGAGACGCCGCTTCTCGCCGCGGCCGAGAAGGGGCACCTTGGTGTGGTGAAGGAGCTCTTGCCTTTCACCACCGATGAGGGGATTTACATGAAGAATAGGATCGGCTACAATCCATTGCATATTGCTGCTAGTCAAGGGCACCATGCCATTATCCAGGTCCTGTTAGACCATGAACCAGGACTTAGCAAAACACTTGGCCCGTCAAATGCAACAGCTCTTGTGGCTGCAGCTACAAGAGGGCATACAGATGTGGTTAAAGAGTTAATGAATCATGATCCTAGCTTGGTAGAAATTTCTAAATCCAATGGTAAGAATGCATTGCATTTTGCGGCACGGCAGGGCCATGTAGATATTGTGAAAGCTTTGCTTGACAAGGATCCACAACTAGTAAGAAGAATTGACAAGAAAGGACAGACTGCTTTGCATATGGCCGTAAAAGGTCATAGCTGTGAGGTGGTGAAGTTGCTACTTGACACAGATTCAGCTCTTGTCATGCTCCCAGACAAGTTCGGCAATTTAGCATTACATGTGGCCACGAGGAAGAAGAGAACAGAGATAGTAAATGAGTTGTTAGCCCTTCGTGACACAAATGTGAATGCTTTAACAAGAGAACAGAAAACACCCCTTGATATAGTTGAAGCACTTCCTCTCTCCGAAGAATCCACGGCAATTAAGGAAAGCTTAACTGAGTATGGTGGTCTCCGAGCCCAGGACCTTAACCGGCCAAGAGATGAGCTTAGAAACACAGTGTCAGCTATAAAAAAAGATGTCCACACTCAGCTGGATCAAGCCCGCAAGACCAGTCGGAGTGTTACTGGAATTGCCAAGGAGATCCGTAGGCTACAGAGGGAAGGAGTCAATATTGCAACTAACTCTGTCACAGTGGTTGCATCGCTCATCGCAACAATAGCATTTGCAGCTATATTTACTGTACCTGGTGGTGATTTAGATTCTGGGAAGTCTGTGGCGGTTTCAACTACATCGTTCAAGATGTTTTATTTTTTTAATGCTATTTCACTCTTCGTAGCATTGGCTGTTGTCGTGGTACAGATCACCCTTATTCGGGGGGAGACGAAGACTGAGAGGAGGGTTATAACAGTGATTAATAAGGCGATGTGGTTGGCCTCTATGACCACTACAGTAGCATTTATTTCTGCAGCATATATAGTGGTTGGTCGACATAATCGGTGGGCTGCAATCTTTATCACAGTTATAGGAGGGATTATAATTTCAGCCGTTCTTTGTATCATGACATATTTTGTGATAAAGTCCAAAAAGATTCGTAGACTGAGAAAGAGAGAGAAGCTTCTTACAAGGACTGGATCCTCCTTGCACTCAGACTCTGGTTCAGAAGTGAATCGTGTTATGGCCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lysine_decarbox

Protein Analysis

618

Amino Acids

67.18

Weight (kDa)

9.7

Isoelectric Point (pI)

34.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 109 - 218 1e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 164 - 215 1.8e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 228 - 287 2.7e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 234 - 283 4.8e-08 Ankyrin repeats (many copies)
Ank_4 PF13637 267 - 317 2.1e-10 Ankyrin repeats (many copies)
Ank_5 PF13857 282 - 317 9.4e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 293 - 359 7e-10 Ankyrin repeats (3 copies)
PGG PF13962 446 - 550 1.2e-21 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011648)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G35990 AT2G35990 AT2G35990 AT5G06300
fragaria_vesca FvH4_6g20040 FvH4_6g20040 FvH4_6g20040
malus_domestica MD12G1037500.v1.1 MD14G1036500.v1.1
prunus_persica Prupe.7G097700_v2.0.a1 Prupe.7G097700_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475501
rosa_laevigata RLG00000023840
rosa_multiflora Rmu_sc0000539.1_g000004
rosa_roxburghii Rroxscaffold_6G00406230
rosa_rugosa Rorug03G0149300 Rorug03G0149400
rosa_samantha Rh3BG229300
rosa_wichuraiana Rw3G018090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1574, 1706
AarI CACCTGC 1 cut(s) 286
AatII GACGTC 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 286
AccB1I GGYRCC 3 cut(s) 130, 513, 618
AccI GTMKAC 2 cut(s) 1657, 1765
AccII CGCG 1 cut(s) 500
AclWI GGATC 9 cut(s) 279, 587, 749, 851, 864, 1272, 1306, 1797, 1810
AcoI YGGCCR 5 cut(s) 304, 501, 909, 1011, 1202
AcsI RAATTY 4 cut(s) 172, 199, 253, 771
AcyI GRCGYC 3 cut(s) 131, 351, 485
AfaI GTAC 3 cut(s) 287, 1413, 1527
AfiI CCNNNNNNNGG 9 cut(s) 58, 334, 411, 510, 523, 558, 1189, 1542, 1543
AflIII ACRYGT 1 cut(s) 1006
AgsI TTSAA 4 cut(s) 313, 1118, 1455, 1468
AhlI ACTAGT 1 cut(s) 865
AjnI CCWGG 6 cut(s) 51, 330, 633, 655, 1188, 1414
AleI CACNNNNGTG 2 cut(s) 519, 1070
Alw21I GWGCWC 2 cut(s) 327, 537
Alw26I GTCTC 7 cut(s) 162, 189, 216, 243, 476, 1184, 1544
AlwI GGATC 9 cut(s) 279, 587, 749, 851, 864, 1272, 1306, 1797, 1810
AlwNI CAGNNNCTG 2 cut(s) 640, 1823
AoxI GGCC 9 cut(s) 304, 501, 676, 820, 909, 1011, 1202, 1602, 1848
ApeKI GCWGC 7 cut(s) 64, 605, 704, 707, 1397, 1637, 1673
ApoI RAATTY 4 cut(s) 172, 199, 253, 771
AseI ATTAAT 1 cut(s) 1584
Asp700I GAANNNNTTC 1 cut(s) 1122
AspLEI GCGC 1 cut(s) 133
AspS9I GGNCC 7 cut(s) 49, 402, 637, 677, 820, 1192, 1849
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 8 cut(s) 53, 102, 310, 538, 538, 949, 1433, 1525
AsuII TTCGAA 2 cut(s) 170, 197
AvaII GGWCC 4 cut(s) 49, 402, 637, 1192
BaeGI GKGCMC 2 cut(s) 516, 621
BalI TGGCCA 1 cut(s) 1013
BamHI GGATCC 2 cut(s) 856, 1802
BanI GGYRCC 3 cut(s) 130, 513, 618
BanII GRGCYC 4 cut(s) 75, 327, 537, 1189
BarI GAAGNNNNNNTAC 2 cut(s) 269, 301
BauI CACGAG 2 cut(s) 406, 1015
BbsI GAAGAC 1 cut(s) 1562
Bbv12I GWGCWC 2 cut(s) 327, 537
BbvI GCAGC 7 cut(s) 51, 592, 691, 719, 1409, 1649, 1660
BccI CCATC 2 cut(s) 211, 391
BceAI ACGGC 6 cut(s) 118, 344, 830, 896, 1161, 1700
BcgI CGANNNNNNTGC 2 cut(s) 368, 402
BciT130I CCWGG 6 cut(s) 53, 332, 635, 657, 1190, 1416
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 7 cut(s) 162, 189, 216, 243, 476, 1184, 1544
BcuI ACTAGT 1 cut(s) 865
BfaI CTAG 4 cut(s) 177, 609, 759, 866
BfmI CTRYAG 4 cut(s) 705, 1322, 1617, 1635
BfoI RGCGCY 1 cut(s) 134
BfuAI ACCTGC 1 cut(s) 286
Bme1390I CCNGG 7 cut(s) 53, 84, 332, 635, 657, 1190, 1416
Bme18I GGWCC 4 cut(s) 49, 402, 637, 1192
BmgT120I GGNCC 7 cut(s) 49, 402, 637, 677, 820, 1192, 1849
BmiI GGNNCC 9 cut(s) 50, 72, 99, 132, 403, 515, 620, 858, 1804
BmrFI CCNGG 7 cut(s) 53, 84, 332, 635, 657, 1190, 1416
BmsI GCATC 4 cut(s) 22, 376, 415, 1379
BpiI GAAGAC 1 cut(s) 1562
BpmI CTGGAG 2 cut(s) 74, 89
Bpu14I TTCGAA 2 cut(s) 170, 197
BpuMI CCSGG 1 cut(s) 84
BsaAI YACGTR 1 cut(s) 289
BsaHI GRCGYC 3 cut(s) 131, 351, 485
BsaI GGTCTC 1 cut(s) 1184
BsaJI CCNNGG 5 cut(s) 498, 517, 1143, 1188, 1305
Bsc4I CCNNNNNNNGG 9 cut(s) 58, 334, 411, 510, 523, 558, 1189, 1542, 1543
Bse118I RCCGGY 2 cut(s) 302, 1200
Bse1I ACTGG 3 cut(s) 1281, 1300, 1804
Bse3DI GCAATG 2 cut(s) 593, 797
BseBI CCWGG 6 cut(s) 53, 332, 635, 657, 1190, 1416
BseDI CCNNGG 5 cut(s) 498, 517, 1143, 1188, 1305
BseLI CCNNNNNNNGG 9 cut(s) 58, 334, 411, 510, 523, 558, 1189, 1542, 1543
BseMI GCAATG 2 cut(s) 593, 797
BseMII CTCAG 7 cut(s) 408, 429, 1158, 1271, 1551, 1760, 1830
BseNI ACTGG 3 cut(s) 1281, 1300, 1804
BseRI GAGGAG 1 cut(s) 1579
BseSI GKGCMC 2 cut(s) 516, 621
BseX3I CGGCCG 2 cut(s) 304, 501
BseXI GCAGC 7 cut(s) 51, 592, 691, 719, 1409, 1649, 1660
Bsh1236I CGCG 1 cut(s) 500
Bsh1285I CGRYCG 3 cut(s) 95, 307, 504
BshFI GGCC 9 cut(s) 306, 503, 678, 822, 911, 1013, 1204, 1604, 1850
BshNI GGYRCC 3 cut(s) 130, 513, 618
BsiEI CGRYCG 3 cut(s) 95, 307, 504
BsiHKAI GWGCWC 2 cut(s) 327, 537
BsiSI CCGG 5 cut(s) 83, 204, 258, 303, 1201
BslFI GGGAC 3 cut(s) 39, 62, 415
BslI CCNNNNNNNGG 9 cut(s) 58, 334, 411, 510, 523, 558, 1189, 1542, 1543
BsmAI GTCTC 7 cut(s) 162, 189, 216, 243, 476, 1184, 1544
BsmBI CGTCTC 2 cut(s) 476, 1544
BsmFI GGGAC 3 cut(s) 39, 62, 415
BsmI GAATGC 2 cut(s) 799, 1081
BsnI GGCC 9 cut(s) 306, 503, 678, 822, 911, 1013, 1204, 1604, 1850
Bso31I GGTCTC 1 cut(s) 1184
Bsp119I TTCGAA 2 cut(s) 170, 197
Bsp1286I GDGCHC 6 cut(s) 75, 327, 516, 537, 621, 1189
Bsp143I GATC 9 cut(s) 92, 271, 579, 754, 856, 1264, 1311, 1530, 1802
BspANI GGCC 9 cut(s) 306, 503, 678, 822, 911, 1013, 1204, 1604, 1850
BspCNI CTCAG 7 cut(s) 409, 430, 1159, 1270, 1552, 1761, 1829
BspFNI CGCG 1 cut(s) 500
BspHI TCATGA 2 cut(s) 751, 1728
BspLI GGNNCC 9 cut(s) 50, 72, 99, 132, 403, 515, 620, 858, 1804
BspMAI CTGCAG 2 cut(s) 709, 1639
BspMI ACCTGC 1 cut(s) 286
BspPI GGATC 9 cut(s) 279, 587, 749, 851, 864, 1272, 1306, 1797, 1810
BspT104I TTCGAA 2 cut(s) 170, 197
BspT107I GGYRCC 3 cut(s) 130, 513, 618
BspTNI GGTCTC 1 cut(s) 1184
BsrDI GCAATG 2 cut(s) 593, 797
BsrFI RCCGGY 2 cut(s) 302, 1200
BsrI ACTGG 3 cut(s) 1281, 1300, 1804
BssAI RCCGGY 2 cut(s) 302, 1200
BssECI CCNNGG 5 cut(s) 498, 517, 1143, 1188, 1305
BssMI GATC 9 cut(s) 92, 271, 579, 754, 856, 1264, 1311, 1530, 1802
BssNI GRCGYC 3 cut(s) 131, 351, 485
BssSI CACGAG 2 cut(s) 406, 1015
BssT1I CCWWGG 2 cut(s) 517, 1305
Bst2BI CACGAG 2 cut(s) 406, 1015
Bst2UI CCWGG 6 cut(s) 53, 332, 635, 657, 1190, 1416
Bst4CI ACNGT 6 cut(s) 1228, 1363, 1411, 1579, 1621, 1690
Bst6I CTCTTC 2 cut(s) 1019, 1505
BstACI GRCGYC 3 cut(s) 131, 351, 485
BstBAI YACGTR 1 cut(s) 289
BstBI TTCGAA 2 cut(s) 170, 197
BstC8I GCNNGC 2 cut(s) 129, 1273
BstDEI CTNAG 9 cut(s) 417, 438, 662, 1167, 1217, 1257, 1560, 1769, 1816
BstDSI CCRYGG 2 cut(s) 498, 1143
BstEII GGTNACC 1 cut(s) 298
BstENI CCTNNNNNAGG 1 cut(s) 56
BstFNI CGCG 1 cut(s) 500
BstH2I RGCGCY 1 cut(s) 134
BstHHI GCGC 1 cut(s) 133
BstKTI GATC 9 cut(s) 95, 274, 582, 757, 859, 1267, 1314, 1533, 1805
BstMAI GTCTC 7 cut(s) 162, 189, 216, 243, 476, 1184, 1544
BstMBI GATC 9 cut(s) 92, 271, 579, 754, 856, 1264, 1311, 1530, 1802
BstMCI CGRYCG 3 cut(s) 95, 307, 504
BstMWI GCNNNNNNNGC 6 cut(s) 70, 79, 434, 701, 1388, 1397
BstNI CCWGG 6 cut(s) 53, 332, 635, 657, 1190, 1416
BstNSI RCATGY 1 cut(s) 1010
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 7 cut(s) 51, 82, 330, 633, 655, 1188, 1414
BstSFI CTRYAG 4 cut(s) 705, 1322, 1617, 1635
BstSLI GKGCMC 2 cut(s) 516, 621
BstUI CGCG 1 cut(s) 500
BstV1I GCAGC 7 cut(s) 51, 592, 691, 719, 1409, 1649, 1660
BstV2I GAAGAC 1 cut(s) 1562
BstX2I RGATCY 3 cut(s) 856, 1311, 1802
BstYI RGATCY 3 cut(s) 856, 1311, 1802
BstZI CGGCCG 2 cut(s) 304, 501
BsuRI GGCC 9 cut(s) 306, 503, 678, 822, 911, 1013, 1204, 1604, 1850
BtgI CCRYGG 2 cut(s) 498, 1143
BtgZI GCGATG 3 cut(s) 1356, 1363, 1607
BtsIMutI CAGTG 3 cut(s) 1233, 1368, 1584
BveI ACCTGC 1 cut(s) 286
Cac8I GCNNGC 2 cut(s) 129, 1273
CaiI CAGNNNCTG 2 cut(s) 640, 1823
CciI TCATGA 2 cut(s) 751, 1728
CfoI GCGC 1 cut(s) 133
Cfr10I RCCGGY 2 cut(s) 302, 1200
Cfr13I GGNCC 7 cut(s) 49, 402, 637, 677, 820, 1192, 1849
Cfr42I CCGCGG 1 cut(s) 501
CseI GACGC 1 cut(s) 493
CsiI ACCWGGT 1 cut(s) 1414
Csp6I GTAC 3 cut(s) 286, 1412, 1526
CviAII CATG 8 cut(s) 568, 623, 650, 752, 824, 973, 1007, 1729
CviQI GTAC 3 cut(s) 286, 1412, 1526
DdeI CTNAG 9 cut(s) 417, 438, 662, 1167, 1217, 1257, 1560, 1769, 1816
DinI GGCGCC 1 cut(s) 132
DpnI GATC 9 cut(s) 94, 273, 581, 756, 858, 1266, 1313, 1532, 1804
DpnII GATC 9 cut(s) 92, 271, 579, 754, 856, 1264, 1311, 1530, 1802
EaeI YGGCCR 5 cut(s) 304, 501, 909, 1011, 1202
EagI CGGCCG 2 cut(s) 304, 501
Eam1104I CTCTTC 2 cut(s) 1019, 1505
EarI CTCTTC 2 cut(s) 1019, 1505
Ecl136II GAGCTC 2 cut(s) 325, 535
EclXI CGGCCG 2 cut(s) 304, 501
Eco130I CCWWGG 2 cut(s) 517, 1305
Eco24I GRGCYC 4 cut(s) 75, 327, 537, 1189
Eco31I GGTCTC 1 cut(s) 1184
Eco47I GGWCC 4 cut(s) 49, 402, 637, 1192
Eco52I CGGCCG 2 cut(s) 304, 501
Eco53kI GAGCTC 2 cut(s) 325, 535
Eco91I GGTNACC 1 cut(s) 298
EcoICRI GAGCTC 2 cut(s) 325, 535
EcoNI CCTNNNNNAGG 1 cut(s) 56
EcoO109I RGGNCCY 3 cut(s) 49, 637, 1192
EcoO65I GGTNACC 1 cut(s) 298
EcoRI GAATTC 3 cut(s) 172, 199, 253
EcoRII CCWGG 6 cut(s) 51, 330, 633, 655, 1188, 1414
EcoT14I CCWWGG 2 cut(s) 517, 1305
EcoT22I ATGCAT 1 cut(s) 799
EcoT38I GRGCYC 4 cut(s) 75, 327, 537, 1189
EgeI GGCGCC 1 cut(s) 132
EheI GGCGCC 1 cut(s) 132
ErhI CCWWGG 2 cut(s) 517, 1305
Esp3I CGTCTC 2 cut(s) 476, 1544
FaeI CATG 8 cut(s) 571, 626, 653, 755, 827, 976, 1010, 1732
FalI AAGNNNNNCTT 6 cut(s) 521, 553, 831, 863, 1145, 1177
FaqI GGGAC 3 cut(s) 39, 62, 415
FatI CATG 8 cut(s) 567, 622, 649, 751, 823, 972, 1006, 1728
FauI CCCGC 2 cut(s) 336, 1280
FauNDI CATATG 1 cut(s) 906
FblI GTMKAC 2 cut(s) 1657, 1765
FriOI GRGCYC 4 cut(s) 75, 327, 537, 1189
FspBI CTAG 4 cut(s) 177, 609, 759, 866
GlaI GCGC 1 cut(s) 132
GsuI CTGGAG 2 cut(s) 74, 89
HaeII RGCGCY 1 cut(s) 134
HaeIII GGCC 9 cut(s) 306, 503, 678, 822, 911, 1013, 1204, 1604, 1850
HapII CCGG 5 cut(s) 83, 204, 258, 303, 1201
HgaI GACGC 1 cut(s) 493
HhaI GCGC 1 cut(s) 133
Hin1I GRCGYC 3 cut(s) 131, 351, 485
Hin1II CATG 8 cut(s) 571, 626, 653, 755, 827, 976, 1010, 1732
Hin6I GCGC 1 cut(s) 131
HinP1I GCGC 1 cut(s) 131
HincII GTYRAC 1 cut(s) 1658
HindII GTYRAC 1 cut(s) 1658
HindIII AAGCTT 3 cut(s) 840, 1159, 1784
HinfI GANTC 8 cut(s) 748, 959, 1139, 1335, 1430, 1759, 1820, 1837
HpaII CCGG 5 cut(s) 83, 204, 258, 303, 1201
HphI GGTGA 8 cut(s) 53, 102, 310, 538, 538, 949, 1433, 1525
Hpy166II GTNNAC 3 cut(s) 1252, 1658, 1766
Hpy188I TCNGA 8 cut(s) 97, 216, 276, 1135, 1184, 1287, 1819, 1831
Hpy188III TCNNGA 4 cut(s) 752, 1061, 1468, 1729
Hpy8I GTNNAC 3 cut(s) 1252, 1658, 1766
Hpy99I CGWCG 2 cut(s) 353, 356
HpyAV CCTTC 5 cut(s) 52, 502, 523, 1067, 1325
HpyCH4III ACNGT 6 cut(s) 1228, 1363, 1411, 1579, 1621, 1690
HpyCH4IV ACGT 2 cut(s) 288, 351
HpyF10VI GCNNNNNNNGC 6 cut(s) 70, 79, 434, 701, 1388, 1397
HpyF3I CTNAG 9 cut(s) 417, 438, 662, 1167, 1217, 1257, 1560, 1769, 1816
HpySE526I ACGT 2 cut(s) 288, 351
Hsp92I GRCGYC 3 cut(s) 131, 351, 485
Hsp92II CATG 8 cut(s) 571, 626, 653, 755, 827, 976, 1010, 1732
HspAI GCGC 1 cut(s) 131
KasI GGCGCC 1 cut(s) 130
KspI CCGCGG 1 cut(s) 501
Kzo9I GATC 9 cut(s) 92, 271, 579, 754, 856, 1264, 1311, 1530, 1802
LmnI GCTCC 6 cut(s) 70, 97, 322, 330, 532, 981
Lsp1109I GCAGC 7 cut(s) 51, 592, 691, 719, 1409, 1649, 1660
LweI GCATC 4 cut(s) 22, 376, 415, 1379
MabI ACCWGGT 1 cut(s) 1414
MaeI CTAG 4 cut(s) 177, 609, 759, 866
MaeII ACGT 2 cut(s) 288, 351
MaeIII GTNAC 5 cut(s) 108, 298, 1061, 1291, 1357
MalI GATC 9 cut(s) 94, 273, 581, 756, 858, 1266, 1313, 1532, 1804
MboI GATC 9 cut(s) 92, 271, 579, 754, 856, 1264, 1311, 1530, 1802
MboII GAAGA 9 cut(s) 289, 292, 583, 885, 1033, 1036, 1148, 1492, 1567
MflI RGATCY 3 cut(s) 856, 1311, 1802
MhlI GDGCHC 6 cut(s) 75, 327, 516, 537, 621, 1189
MlsI TGGCCA 1 cut(s) 1013
MluNI TGGCCA 1 cut(s) 1013
Mly113I GGCGCC 1 cut(s) 131
MlyI GAGTC 2 cut(s) 1344, 1814
MmeI TCCRAC 1 cut(s) 1265
Mox20I TGGCCA 1 cut(s) 1013
Mph1103I ATGCAT 1 cut(s) 799
MroXI GAANNNNTTC 1 cut(s) 1122
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 9 cut(s) 735, 743, 1082, 1152, 1163, 1197, 1485, 1584, 1855
MslI CAYNNNNRTG 4 cut(s) 519, 726, 927, 1070
Msp20I TGGCCA 1 cut(s) 1013
MspA1I CMGCKG 2 cut(s) 500, 1261
MspI CCGG 5 cut(s) 83, 204, 258, 303, 1201
MspR9I CCNGG 7 cut(s) 53, 84, 332, 635, 657, 1190, 1416
Mva1269I GAATGC 2 cut(s) 799, 1081
MvaI CCWGG 6 cut(s) 53, 332, 635, 657, 1190, 1416
MvnI CGCG 1 cut(s) 500
MwoI GCNNNNNNNGC 6 cut(s) 70, 79, 434, 701, 1388, 1397
NarI GGCGCC 1 cut(s) 131
NciI CCSGG 1 cut(s) 84
NdeI CATATG 1 cut(s) 906
NdeII GATC 9 cut(s) 92, 271, 579, 754, 856, 1264, 1311, 1530, 1802
NlaIII CATG 8 cut(s) 571, 626, 653, 755, 827, 976, 1010, 1732
NlaIV GGNNCC 9 cut(s) 50, 72, 99, 132, 403, 515, 620, 858, 1804
NmeAIII GCCGAG 1 cut(s) 529
NmuCI GTSAC 4 cut(s) 108, 298, 1061, 1357
NsiI ATGCAT 1 cut(s) 799
NspI RCATGY 1 cut(s) 1010
NspV TTCGAA 2 cut(s) 170, 197
OliI CACNNNNGTG 2 cut(s) 519, 1070
PagI TCATGA 2 cut(s) 751, 1728
PaqCI CACCTGC 1 cut(s) 286
PciI ACATGT 1 cut(s) 1006
PcsI WCGNNNNNNNCGW 3 cut(s) 101, 357, 501
PctI GAATGC 2 cut(s) 799, 1081
PdmI GAANNNNTTC 1 cut(s) 1122
PfeI GAWTC 6 cut(s) 748, 959, 1139, 1430, 1759, 1837
Ple19I CGATCG 1 cut(s) 95
PleI GAGTC 2 cut(s) 1343, 1814
PluTI GGCGCC 1 cut(s) 134
PpsI GAGTC 2 cut(s) 1343, 1814
Ppu21I YACGTR 1 cut(s) 289
PpuMI RGGWCCY 3 cut(s) 49, 637, 1192
PscI ACATGT 1 cut(s) 1006
PshBI ATTAAT 1 cut(s) 1584
PsiI TTATAA 2 cut(s) 1574, 1706
Psp124BI GAGCTC 2 cut(s) 327, 537
Psp5II RGGWCCY 3 cut(s) 49, 637, 1192
Psp6I CCWGG 6 cut(s) 51, 330, 633, 655, 1188, 1414
PspEI GGTNACC 1 cut(s) 298
PspGI CCWGG 6 cut(s) 51, 330, 633, 655, 1188, 1414
PspN4I GGNNCC 9 cut(s) 50, 72, 99, 132, 403, 515, 620, 858, 1804
PspPI GGNCC 7 cut(s) 49, 402, 637, 677, 820, 1192, 1849
PspPPI RGGWCCY 3 cut(s) 49, 637, 1192
PstI CTGCAG 2 cut(s) 709, 1639
PstNI CAGNNNCTG 2 cut(s) 640, 1823
PsuI RGATCY 3 cut(s) 856, 1311, 1802
PvuI CGATCG 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 1261
RsaI GTAC 3 cut(s) 287, 1413, 1527
RsaNI GTAC 3 cut(s) 286, 1412, 1526
RseI CAYNNNNRTG 4 cut(s) 519, 726, 927, 1070
SacI GAGCTC 2 cut(s) 327, 537
SacII CCGCGG 1 cut(s) 501
SalI GTCGAC 1 cut(s) 1656
SaqAI TTAA 9 cut(s) 735, 743, 1082, 1152, 1163, 1197, 1485, 1584, 1855
Sau3AI GATC 9 cut(s) 92, 271, 579, 754, 856, 1264, 1311, 1530, 1802
Sau96I GGNCC 7 cut(s) 49, 402, 637, 677, 820, 1192, 1849
SchI GAGTC 2 cut(s) 1344, 1814
ScrFI CCNGG 7 cut(s) 53, 84, 332, 635, 657, 1190, 1416
SduI GDGCHC 6 cut(s) 75, 327, 516, 537, 621, 1189
SexAI ACCWGGT 1 cut(s) 1414
SfaNI GCATC 4 cut(s) 22, 376, 415, 1379
SfcI CTRYAG 4 cut(s) 705, 1322, 1617, 1635
SfoI GGCGCC 1 cut(s) 132
Sfr303I CCGCGG 1 cut(s) 501
SfuI TTCGAA 2 cut(s) 170, 197
SgrBI CCGCGG 1 cut(s) 501
SinI GGWCC 4 cut(s) 49, 402, 637, 1192
SmiMI CAYNNNNRTG 4 cut(s) 519, 726, 927, 1070
SpeI ACTAGT 1 cut(s) 865
SspDI GGCGCC 1 cut(s) 130
SspI AATATT 1 cut(s) 1342
SspMI CTAG 4 cut(s) 177, 609, 759, 866
SstI GAGCTC 2 cut(s) 327, 537
StyD4I CCNGG 7 cut(s) 51, 82, 330, 633, 655, 1188, 1414
StyI CCWWGG 2 cut(s) 517, 1305
TaaI ACNGT 6 cut(s) 1228, 1363, 1411, 1579, 1621, 1690
TaiI ACGT 2 cut(s) 291, 354
TaqI TCGA 6 cut(s) 91, 122, 170, 197, 251, 1657
TauI GCSGC 9 cut(s) 103, 295, 309, 338, 359, 490, 500, 503, 812
TfiI GAWTC 6 cut(s) 748, 959, 1139, 1430, 1759, 1837
Tru1I TTAA 9 cut(s) 735, 743, 1082, 1152, 1163, 1197, 1485, 1584, 1855
Tru9I TTAA 9 cut(s) 735, 743, 1082, 1152, 1163, 1197, 1485, 1584, 1855
TscAI CASTG 3 cut(s) 1233, 1368, 1584
TseFI GTSAC 4 cut(s) 108, 298, 1061, 1357
TseI GCWGC 7 cut(s) 64, 605, 704, 707, 1397, 1637, 1673
Tsp45I GTSAC 4 cut(s) 108, 298, 1061, 1357
TspDTI ATGAA 3 cut(s) 584, 666, 761
TspGWI ACGGA 2 cut(s) 335, 1304
TspRI CASTG 3 cut(s) 1233, 1368, 1584
VpaK11BI GGWCC 4 cut(s) 49, 402, 637, 1192
VspI ATTAAT 1 cut(s) 1584
XagI CCTNNNNNAGG 1 cut(s) 56
XapI RAATTY 4 cut(s) 172, 199, 253, 771
XceI RCATGY 1 cut(s) 1010
XcmI CCANNNNNNNNNTGG 1 cut(s) 238
XmiI GTMKAC 2 cut(s) 1657, 1765
XmnI GAANNNNTTC 1 cut(s) 1122
XspI CTAG 4 cut(s) 177, 609, 759, 866
ZraI GACGTC 1 cut(s) 352
Zsp2I ATGCAT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.