AT2G37150

Zinc finger, C3HC4 type (RING finger)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002650
Physical Location & Seq
Reverse (-)
15603142 .. 15608587
5446 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G37150.5

Sequence Viewer

Length: 1641 bp
ATGGGGCACAGACATTTTCTTGGTTCATCCCAGTTTTTTGATGATGAACATGATCAAGGCTGGAATCATACACACCCAGAACATCCATATTCGAGTCAAGCAACATCTGGGACCAGTGAGAACAGATCACATGTTTATCCAGCAGAAAACATGTTGAATGAAGGAATGCCAGTGTCTTCTCATTGGAACTCTTCTCCAGGACCAAATGCTTATACTGACTCAGGTCATAGTGTGGAGAGACCACACTACAATCCGGGGGTTTCGGGGCCGTCCCATGATCCTTCCGTGAATTCAACAGTTCCAACTTTCTCTGCTCCACATGAGAATTATGTGACATTTGCATCTTCTTCAAGCTACAACAGCCAGACATGGTCAAATGCTAGTTATGTTGATCATCAATCTATGGAAAGTGTGAGAGGAGCACAAAAGAGAAAACGACCATGTCCTTCTTCCATCTATGAAATGGGTAGTTCAAGCCAATATCACGGTGACAGAACTCCTGCAGACACTCATTTCCCCTCGGAATTGCACTTGGGAAAATCAATAACGCATGATCATGACCCTCATTACATGCCATGGCTTATGAACCCAACTTACAGAAGTAATAATCTCTCGATCAGGGGAGAGAGCTCTTCAAGGAATGTCCGGAGTCGTCCTACACTTGATTCAGAGACCAGCTTAGGTAGAAATAATTTGCCAAGAAGCTTGAGCCTTGATTCTCATTCTACACACCACCACAGTGTTGATCATTATGGCTCAGGTCAATTTCCTGGTCAGACATCTCACGGAAATAAAGACTGGAACTGTGCTAGATTGTCCCCAGTTCTTAGAGATATAAATGGCTTTAGTCCAGAGACAAATAATTTCCTTCCTGCAAGAAGTGTTGTCAACAGCTTATCTGTTAATACTTCCGGCTATCATCACCATGAGCTTACCGGAAACAGAAACCCTACAGTTTCCCACGGGGTTCCTGGAACTTCAACACTATCTACAAGCAGCAGCCGCTTCTCTCATAGGTCAACATCCACCTATAGATCTTCTTCACACGGTTCGCGATCGGGACATGTAGCATCTTCTTCTGGAGACAGGTCTCATTTGGTCACTGAGACTTATCCGTCTAGGCATTTAAGGCCACCACCACACATTTCATGGCGTAGCGGTGATCGGCCAGGGAGACGCAGAAGTTCCTATGAGAGGTTTCAGCCTCCTTTCGATGAAGTTGCTCTCCACGAACGGTTTTCATCTGAGGAATTCATGGTTGAAGATCGCCAACCACAATACTACGGATCCAGAAACATGCTTGATCACCACAGAGACATGAGGCTTGACATCGATAACATGAGCTATGAGGAACTCCTTGATCTTGGAGAAAGGATTGGGAGTGTCAACACTGGTCTATCCGACAGCGCAATCTCCAGTTGCTTGTTAGCAACAATGTATTATCCATCATATCAAACAGAGGAGCAAAGAAAATGTGCAATCTGCCTGGAGGAGTACAAGGAAAAAGAAGAGTTAGGGGAAGTTAAGGGGTGTGGTCATGACTACCATGGGAGGTGCATCAAGAAATGGTTGTCTATGAAGAACTCTTGCCCTATTTGCAAATCCCCTGCTTTACCTGATGCTTCTAAGAACTCATCATAA

Protein Analysis

546

Amino Acids

61.22

Weight (kDa)

6.65

Isoelectric Point (pI)

63.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 491 - 534 1.2e-12 Ring finger domain
zf-C3HC4_2 PF13923 491 - 533 8.9e-09 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 491 - 534 1.4e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 492 - 533 1.5e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 492 - 520 3.3e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1114
AccII CGCG 1 cut(s) 1054
AccIII TCCGGA 1 cut(s) 645
AciI CCGC 2 cut(s) 1003, 1158
AclWI GGATC 3 cut(s) 272, 1281, 1294
AcoI YGGCCR 1 cut(s) 1166
AcsI RAATTY 2 cut(s) 289, 1250
AfaI GTAC 1 cut(s) 1496
AfiI CCNNNNNNNGG 1 cut(s) 1194
AflIII ACRYGT 3 cut(s) 130, 150, 1063
AgsI TTSAA 7 cut(s) 157, 294, 351, 474, 636, 981, 1262
AjnI CCWGG 5 cut(s) 196, 769, 970, 1168, 1485
AleI CACNNNNGTG 1 cut(s) 738
AluBI AGCT 7 cut(s) 354, 630, 678, 705, 894, 931, 1344
AluI AGCT 7 cut(s) 354, 630, 678, 705, 894, 931, 1344
Alw21I GWGCWC 2 cut(s) 424, 632
Alw26I GTCTC 8 cut(s) 232, 665, 848, 1077, 1095, 1100, 1168, 1308
AlwI GGATC 3 cut(s) 272, 1281, 1294
Aor13HI TCCGGA 1 cut(s) 645
AoxI GGCC 3 cut(s) 266, 1130, 1166
ApeKI GCWGC 2 cut(s) 996, 999
ApoI RAATTY 2 cut(s) 289, 1250
AspLEI GCGC 1 cut(s) 1409
AspS9I GGNCC 3 cut(s) 111, 200, 266
AsuC2I CCSGG 1 cut(s) 255
AsuHPI GGTGA 4 cut(s) 500, 914, 1172, 1298
AvaII GGWCC 2 cut(s) 111, 200
BaeGI GKGCMC 1 cut(s) 9
BamHI GGATCC 1 cut(s) 1286
BanII GRGCYC 1 cut(s) 632
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 2 cut(s) 424, 632
BbvI GCAGC 2 cut(s) 1008, 1011
BccI CCATC 2 cut(s) 461, 1453
BceAI ACGGC 1 cut(s) 253
BcgI CGANNNNNNTGC 2 cut(s) 1202, 1236
BciT130I CCWGG 5 cut(s) 198, 771, 972, 1170, 1487
BclI TGATCA 5 cut(s) 52, 391, 553, 745, 1303
BcnI CCSGG 1 cut(s) 255
BcoDI GTCTC 8 cut(s) 232, 665, 848, 1077, 1095, 1100, 1168, 1308
BfaI CTAG 3 cut(s) 381, 810, 1119
BfmI CTRYAG 3 cut(s) 501, 951, 1030
BglII AGATCT 1 cut(s) 1034
BisI GCNGC 3 cut(s) 997, 1000, 1003
BlsI GCNGC 3 cut(s) 998, 1001, 1004
Bme1390I CCNGG 6 cut(s) 198, 255, 771, 972, 1170, 1487
Bme18I GGWCC 2 cut(s) 111, 200
BmgT120I GGNCC 3 cut(s) 111, 200, 266
BmiI GGNNCC 4 cut(s) 112, 267, 969, 1288
BmrFI CCNGG 6 cut(s) 198, 255, 771, 972, 1170, 1487
BmrI ACTGGG 2 cut(s) 25, 815
BmsI GCATC 4 cut(s) 350, 1079, 1566, 1609
BmuI ACTGGG 2 cut(s) 25, 815
BoxI GACNNNNGTC 1 cut(s) 222
BpiI GAAGAC 1 cut(s) 168
BpmI CTGGAG 4 cut(s) 180, 1101, 1399, 1508
Bpu10I CCTNAGC 2 cut(s) 679, 757
BpuEI CTTGAG 1 cut(s) 727
BpuMI CCSGG 1 cut(s) 255
Bsa29I ATCGAT 1 cut(s) 1332
BsaI GGTCTC 3 cut(s) 232, 665, 1095
BsaJI CCNNGG 6 cut(s) 254, 519, 575, 961, 1169, 1546
BsaWI WCCGGW 2 cut(s) 645, 935
Bsc4I CCNNNNNNNGG 1 cut(s) 1194
Bse1I ACTGG 7 cut(s) 31, 114, 170, 803, 821, 1396, 1416
BseAI TCCGGA 1 cut(s) 645
BseBI CCWGG 5 cut(s) 198, 771, 972, 1170, 1487
BseCI ATCGAT 1 cut(s) 1332
BseDI CCNNGG 6 cut(s) 254, 519, 575, 961, 1169, 1546
BseGI GGATG 3 cut(s) 26, 82, 1022
BseLI CCNNNNNNNGG 1 cut(s) 1194
BseMII CTCAG 4 cut(s) 234, 771, 1095, 1236
BseNI ACTGG 7 cut(s) 31, 114, 170, 803, 821, 1396, 1416
BseRI GAGGAG 3 cut(s) 432, 1475, 1505
BseSI GKGCMC 1 cut(s) 9
BseXI GCAGC 2 cut(s) 1008, 1011
Bsh1236I CGCG 1 cut(s) 1054
Bsh1285I CGRYCG 1 cut(s) 1058
BshFI GGCC 3 cut(s) 268, 1132, 1168
BshVI ATCGAT 1 cut(s) 1332
BsiEI CGRYCG 1 cut(s) 1058
BsiHKAI GWGCWC 2 cut(s) 424, 632
BsiSI CCGG 4 cut(s) 254, 646, 912, 936
BslFI GGGAC 4 cut(s) 124, 256, 802, 1074
BslI CCNNNNNNNGG 1 cut(s) 1194
BsmAI GTCTC 8 cut(s) 232, 665, 848, 1077, 1095, 1100, 1168, 1308
BsmBI CGTCTC 1 cut(s) 1168
BsmFI GGGAC 4 cut(s) 124, 256, 802, 1074
BsmI GAATGC 1 cut(s) 171
BsnI GGCC 3 cut(s) 268, 1132, 1168
Bso31I GGTCTC 3 cut(s) 232, 665, 1095
Bsp1286I GDGCHC 3 cut(s) 9, 424, 632
Bsp13I TCCGGA 1 cut(s) 645
Bsp19I CCATGG 2 cut(s) 575, 1546
Bsp68I TCGCGA 1 cut(s) 1054
BspACI CCGC 2 cut(s) 1003, 1158
BspANI GGCC 3 cut(s) 268, 1132, 1168
BspCNI CTCAG 4 cut(s) 233, 770, 1096, 1237
BspDI ATCGAT 1 cut(s) 1332
BspEI TCCGGA 1 cut(s) 645
BspFNI CGCG 1 cut(s) 1054
BspHI TCATGA 2 cut(s) 556, 1537
BspLI GGNNCC 4 cut(s) 112, 267, 969, 1288
BspMAI CTGCAG 1 cut(s) 505
BspPI GGATC 3 cut(s) 272, 1281, 1294
BspQI GCTCTTC 1 cut(s) 637
BspTNI GGTCTC 3 cut(s) 232, 665, 1095
BsrI ACTGG 7 cut(s) 31, 114, 170, 803, 821, 1396, 1416
BssECI CCNNGG 6 cut(s) 254, 519, 575, 961, 1169, 1546
BssT1I CCWWGG 2 cut(s) 575, 1546
Bst2UI CCWGG 5 cut(s) 198, 771, 972, 1170, 1487
Bst4CI ACNGT 7 cut(s) 298, 488, 740, 806, 955, 1049, 1236
Bst6I CTCTTC 3 cut(s) 196, 637, 1503
BstDEI CTNAG 7 cut(s) 220, 679, 757, 827, 1104, 1245, 1626
BstDSI CCRYGG 3 cut(s) 575, 961, 1546
BstENI CCTNNNNNAGG 1 cut(s) 1192
BstF5I GGATG 3 cut(s) 26, 82, 1022
BstFNI CGCG 1 cut(s) 1054
BstHHI GCGC 1 cut(s) 1409
BstMAI GTCTC 8 cut(s) 232, 665, 848, 1077, 1095, 1100, 1168, 1308
BstMCI CGRYCG 1 cut(s) 1058
BstMWI GCNNNNNNNGC 4 cut(s) 360, 1002, 1129, 1596
BstNI CCWGG 5 cut(s) 198, 771, 972, 1170, 1487
BstNSI RCATGY 5 cut(s) 134, 154, 574, 1067, 1300
BstPAI GACNNNNGTC 1 cut(s) 222
BstSCI CCNGG 6 cut(s) 196, 253, 769, 970, 1168, 1485
BstSFI CTRYAG 3 cut(s) 501, 951, 1030
BstSLI GKGCMC 1 cut(s) 9
BstUI CGCG 1 cut(s) 1054
BstV1I GCAGC 2 cut(s) 1008, 1011
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 2 cut(s) 1034, 1286
BstYI RGATCY 2 cut(s) 1034, 1286
Bsu15I ATCGAT 1 cut(s) 1332
BsuRI GGCC 3 cut(s) 268, 1132, 1168
BsuTUI ATCGAT 1 cut(s) 1332
BtgI CCRYGG 3 cut(s) 575, 961, 1546
BtsCI GGATG 3 cut(s) 26, 82, 1022
BtsIMutI CAGTG 5 cut(s) 121, 177, 745, 1101, 1389
BtuMI TCGCGA 1 cut(s) 1054
CciI TCATGA 2 cut(s) 556, 1537
CfoI GCGC 1 cut(s) 1409
Cfr13I GGNCC 3 cut(s) 111, 200, 266
ClaI ATCGAT 1 cut(s) 1332
CseI GACGC 1 cut(s) 1185
Csp6I GTAC 1 cut(s) 1495
CviQI GTAC 1 cut(s) 1495
DdeI CTNAG 7 cut(s) 220, 679, 757, 827, 1104, 1245, 1626
DrdI GACNNNNNNGTC 1 cut(s) 1114
DseDI GACNNNNNNGTC 1 cut(s) 1114
EaeI YGGCCR 1 cut(s) 1166
Eam1104I CTCTTC 3 cut(s) 196, 637, 1503
EarI CTCTTC 3 cut(s) 196, 637, 1503
Ecl136II GAGCTC 1 cut(s) 630
Eco130I CCWWGG 2 cut(s) 575, 1546
Eco24I GRGCYC 1 cut(s) 632
Eco31I GGTCTC 3 cut(s) 232, 665, 1095
Eco47I GGWCC 2 cut(s) 111, 200
Eco53kI GAGCTC 1 cut(s) 630
EcoICRI GAGCTC 1 cut(s) 630
EcoNI CCTNNNNNAGG 1 cut(s) 1192
EcoRI GAATTC 2 cut(s) 289, 1250
EcoRII CCWGG 5 cut(s) 196, 769, 970, 1168, 1485
EcoT14I CCWWGG 2 cut(s) 575, 1546
EcoT38I GRGCYC 1 cut(s) 632
ErhI CCWWGG 2 cut(s) 575, 1546
Esp3I CGTCTC 1 cut(s) 1168
FaqI GGGAC 4 cut(s) 124, 256, 802, 1074
FbaI TGATCA 5 cut(s) 52, 391, 553, 745, 1303
Fnu4HI GCNGC 3 cut(s) 997, 1000, 1003
FokI GGATG 3 cut(s) 13, 69, 1009
FriOI GRGCYC 1 cut(s) 632
Fsp4HI GCNGC 3 cut(s) 997, 1000, 1003
FspBI CTAG 3 cut(s) 381, 810, 1119
GlaI GCGC 1 cut(s) 1408
GluI GCNGC 3 cut(s) 997, 1000, 1003
GsuI CTGGAG 4 cut(s) 180, 1101, 1399, 1508
HaeIII GGCC 3 cut(s) 268, 1132, 1168
HapII CCGG 4 cut(s) 254, 646, 912, 936
HgaI GACGC 1 cut(s) 1185
HhaI GCGC 1 cut(s) 1409
Hin6I GCGC 1 cut(s) 1407
HinP1I GCGC 1 cut(s) 1407
HincII GTYRAC 3 cut(s) 889, 1020, 1387
HindII GTYRAC 3 cut(s) 889, 1020, 1387
HindIII AAGCTT 1 cut(s) 703
HinfI GANTC 6 cut(s) 64, 94, 218, 649, 665, 716
HpaII CCGG 4 cut(s) 254, 646, 912, 936
HphI GGTGA 4 cut(s) 500, 914, 1172, 1298
Hpy166II GTNNAC 3 cut(s) 889, 1020, 1387
Hpy188I TCNGA 5 cut(s) 523, 670, 777, 1246, 1402
Hpy8I GTNNAC 3 cut(s) 889, 1020, 1387
HpyAV CCTTC 4 cut(s) 155, 291, 456, 878
HpyCH4III ACNGT 7 cut(s) 298, 488, 740, 806, 955, 1049, 1236
HpyCH4V TGCA 7 cut(s) 341, 503, 529, 875, 1478, 1557, 1599
HpyF10VI GCNNNNNNNGC 4 cut(s) 360, 1002, 1129, 1596
HpyF3I CTNAG 7 cut(s) 220, 679, 757, 827, 1104, 1245, 1626
HspAI GCGC 1 cut(s) 1407
Kpn2I TCCGGA 1 cut(s) 645
Ksp22I TGATCA 5 cut(s) 52, 391, 553, 745, 1303
LguI GCTCTTC 1 cut(s) 637
LmnI GCTCC 3 cut(s) 319, 419, 1462
Lsp1109I GCAGC 2 cut(s) 1008, 1011
LweI GCATC 4 cut(s) 350, 1079, 1566, 1609
MaeI CTAG 3 cut(s) 381, 810, 1119
MaeIII GTNAC 3 cut(s) 331, 488, 1099
MflI RGATCY 2 cut(s) 1034, 1286
MhlI GDGCHC 3 cut(s) 9, 424, 632
MluCI AATT 7 cut(s) 289, 325, 524, 691, 764, 862, 1250
MlyI GAGTC 3 cut(s) 103, 212, 658
MmeI TCCRAC 2 cut(s) 326, 1425
MroI TCCGGA 1 cut(s) 645
MseI TTAA 3 cut(s) 903, 1127, 1524
MslI CAYNNNNRTG 3 cut(s) 555, 738, 924
MspI CCGG 4 cut(s) 254, 646, 912, 936
MspR9I CCNGG 6 cut(s) 198, 255, 771, 972, 1170, 1487
Mva1269I GAATGC 1 cut(s) 171
MvaI CCWGG 5 cut(s) 198, 771, 972, 1170, 1487
MvnI CGCG 1 cut(s) 1054
MwoI GCNNNNNNNGC 4 cut(s) 360, 1002, 1129, 1596
NciI CCSGG 1 cut(s) 255
NcoI CCATGG 2 cut(s) 575, 1546
NlaIV GGNNCC 4 cut(s) 112, 267, 969, 1288
NmuCI GTSAC 3 cut(s) 331, 488, 1099
NruI TCGCGA 1 cut(s) 1054
NspI RCATGY 5 cut(s) 134, 154, 574, 1067, 1300
OliI CACNNNNGTG 1 cut(s) 738
PagI TCATGA 2 cut(s) 556, 1537
PciI ACATGT 3 cut(s) 130, 150, 1063
PciSI GCTCTTC 1 cut(s) 637
PctI GAATGC 1 cut(s) 171
PfeI GAWTC 3 cut(s) 64, 665, 716
PflFI GACNNNGTC 2 cut(s) 370, 441
PfoI TCCNGGA 2 cut(s) 196, 970
PkrI GCNGC 3 cut(s) 998, 1001, 1004
Ple19I CGATCG 1 cut(s) 1058
PleI GAGTC 3 cut(s) 102, 212, 657
PpsI GAGTC 3 cut(s) 102, 212, 657
PscI ACATGT 3 cut(s) 130, 150, 1063
PshAI GACNNNNGTC 1 cut(s) 222
Psp124BI GAGCTC 1 cut(s) 632
Psp6I CCWGG 5 cut(s) 196, 769, 970, 1168, 1485
PspGI CCWGG 5 cut(s) 196, 769, 970, 1168, 1485
PspN4I GGNNCC 4 cut(s) 112, 267, 969, 1288
PspPI GGNCC 3 cut(s) 111, 200, 266
PsrI GAACNNNNNNTAC 2 cut(s) 578, 610
PstI CTGCAG 1 cut(s) 505
PsuI RGATCY 2 cut(s) 1034, 1286
PsyI GACNNNGTC 2 cut(s) 370, 441
PvuI CGATCG 1 cut(s) 1058
RruI TCGCGA 1 cut(s) 1054
RsaI GTAC 1 cut(s) 1496
RsaNI GTAC 1 cut(s) 1495
RseI CAYNNNNRTG 3 cut(s) 555, 738, 924
SacI GAGCTC 1 cut(s) 632
SapI GCTCTTC 1 cut(s) 637
SaqAI TTAA 3 cut(s) 903, 1127, 1524
SatI GCNGC 3 cut(s) 997, 1000, 1003
Sau96I GGNCC 3 cut(s) 111, 200, 266
SchI GAGTC 3 cut(s) 103, 212, 658
ScrFI CCNGG 6 cut(s) 198, 255, 771, 972, 1170, 1487
SduI GDGCHC 3 cut(s) 9, 424, 632
SfaNI GCATC 4 cut(s) 350, 1079, 1566, 1609
SfcI CTRYAG 3 cut(s) 501, 951, 1030
SinI GGWCC 2 cut(s) 111, 200
SmiMI CAYNNNNRTG 3 cut(s) 555, 738, 924
SmlI CTYRAG 1 cut(s) 706
SmoI CTYRAG 1 cut(s) 706
Sse9I AATT 7 cut(s) 289, 325, 524, 691, 764, 862, 1250
SsiI CCGC 2 cut(s) 1003, 1158
SspMI CTAG 3 cut(s) 381, 810, 1119
SstI GAGCTC 1 cut(s) 632
StyD4I CCNGG 6 cut(s) 196, 253, 769, 970, 1168, 1485
StyI CCWWGG 2 cut(s) 575, 1546
TaaI ACNGT 7 cut(s) 298, 488, 740, 806, 955, 1049, 1236
TaqI TCGA 4 cut(s) 92, 614, 1212, 1332
TasI AATT 7 cut(s) 289, 325, 524, 691, 764, 862, 1250
TatI WGTACW 1 cut(s) 1494
TauI GCSGC 1 cut(s) 1005
TfiI GAWTC 3 cut(s) 64, 665, 716
Tru1I TTAA 3 cut(s) 903, 1127, 1524
Tru9I TTAA 3 cut(s) 903, 1127, 1524
TscAI CASTG 5 cut(s) 121, 177, 745, 1108, 1396
TseFI GTSAC 3 cut(s) 331, 488, 1099
TseI GCWGC 2 cut(s) 996, 999
Tsp45I GTSAC 3 cut(s) 331, 488, 1099
TspGWI ACGGA 4 cut(s) 274, 801, 1104, 1299
TspRI CASTG 5 cut(s) 121, 177, 745, 1108, 1396
Tth111I GACNNNGTC 2 cut(s) 370, 441
VpaK11BI GGWCC 2 cut(s) 111, 200
XagI CCTNNNNNAGG 1 cut(s) 1192
XapI RAATTY 2 cut(s) 289, 1250
XceI RCATGY 5 cut(s) 134, 154, 574, 1067, 1300
XcmI CCANNNNNNNNNTGG 3 cut(s) 460, 968, 1146
XspI CTAG 3 cut(s) 381, 810, 1119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.