Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr3A

Orthogroup ID

OG0002650

Physical Location & Seq

Genomic Coordinates Reverse (-)

13303533 .. 13310720

Gene Structure 7188 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh3AG150000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1335 bp

ATGGGGCATAGACATCCGTTCAACGCATCTCAAATTTTTGAGAGTGAAAATGATCCGAATTGGAACCATATGAACACAGAACACCCATTTGGACATCTGGCTAGGGCTGGGGCGGGAGAGAATGGTTCTTTGTTTTATCCAGTGGAAAATATGTTCAATGATGGAATGCATTTTCCTTCGAATTGGAACCCGCCTATGAGGTCATATGGGTATTCTCCCTTGAGCCACAGTGTTGATATGCCACCTCAATATCAACCGGATGCTTCTGGTCCATCTCATGATCCTTTTCAACATCCACCAAGTGCTAGTACTTTTGGTATGGCCAGTGGAAACTATGCTCACCATCCATCTTCGTCCAGCTATGACCGTCGAGCATTCCATGGTGTTGAGGGTGGTTTTGTTGATCTCACAATGAGCAATGGAAGAGGGCCACACAAGCGAAAAAGTCCTGGGATCCCTTCTGTAGCAGAGAGAGGCAGCACGAGCAGATACTACAGCGCTGGAAGTTCATCTGACCTCTCTAGATCTACAGAATTACAGCAGGAGAAAGTAAACATAGATACCCCACACATGCCGTGGGATCACATGACTATGATTCACCCCAGCTATAGAGGGAATGACCCAACAAGTAGGGGTGAGGGATCTATGAGAAATGTGAGAAGTCGGTCAGAACATGATTTGGAGTCCAACCTAGCAAGGACACATTTGTCGAACAATCCTATACATAGTTCGTATTCTGCAAATTTCCCTGTTGACCACCCTAGTACGATTGATCTTTCGGGTCAGAGCTCCAATGTTTTAGCCCCTGAATGGAACCATATTAGCGTATCACCTCCTCATGGAAGGATTTTACCTTCAGATACAAGTGGTTTTAGTCATGATCCCAACCACTTCCTTGTAGGCAGCAGTATGGTTAATGCTTCTGCCGAAGCTGGTGTATACCACCATGATTTCATTCCAACCAGGAGTGCTGCTGTTCCTCCAAGTTATCCTGGTGCCTTACCCCATACTGTAAGGGGAGTTCGCAGTAACTATTCTCAAAGATCTAGCCCAACCTTGAGGGCTTCTTCAAGCAGCATGAGGTTTGGGCCTGTGACACATCCAGATGAAGGATTGCAGTTGGTATCTGAGAGTTACCCAAGACATCCTAGGCCAGTGGCTACAGTTGGGTGGCGTAACAGCGACAGGAATGGTAGATCAAGGATGTCTAATGATAGATATCGAGTTTCTGAAGAGACTAGTCTCCATGATCGGTTTTCATCTGAGGTATGTAGCAAAGTTGATGCAGGGTATTTTATCTTGCCACATGTGAGAGAGGGTGCAGCTCCTTTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0051865 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko03013 ko05164 map03013 map05164

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

444

Amino Acids

49.01

Weight (kDa)

6.68

Isoelectric Point (pI)

57.57

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002650)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150 AT2G37150
fragaria_vesca	FvH4_6g15650 FvH4_6g15650 FvH4_6g15650 FvH4_6g15650
malus_domestica	MD04G1100800.v1.1 MD12G1120500.v1.1
prunus_persica	Prupe.6G238800_v2.0.a1
pyrus_communis	pycom04g09560 pycom12g11950
rosa_chinensis	RchiOBHm_Chr3g0469341
rosa_laevigata	RLG00000024358
rosa_multiflora	Rmu_ssc0000394.1_g000001
rosa_roxburghii	Rroxscaffold_6G00411970
rosa_rugosa	Rorug03G0100500 Rorug03G0100600 Rorug03G0100600 Rorug03G0100700 Rorug03G0100700 Rorug03G0100800 Rorug03G0100800
rosa_samantha	Rh3AG150000 Rh3BG173600 Rh3CG164600 Rh3DG201900
rosa_wichuraiana	Rw3G014140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	706
AccB1I	GGYRCC	1 cut(s)	995
AccI	GTMKAC	1 cut(s)	939
AciI	CCGC	2 cut(s)	113, 191
AclWI	GGATC	7 cut(s)	47, 275, 448, 461, 588, 649, 875
AcoI	YGGCCR	1 cut(s)	321
AcsI	RAATTY	2 cut(s)	33, 742
AcuI	CTGAAG	2 cut(s)	840, 1251
AdeI	CACNNNGTG	1 cut(s)	302
AfaI	GTAC	2 cut(s)	310, 766
AfeI	AGCGCT	1 cut(s)	499
AfiI	CCNNNNNNNGG	3 cut(s)	810, 839, 1109
AflIII	ACRYGT	1 cut(s)	1306
AgsI	TTSAA	4 cut(s)	22, 157, 290, 1071
AhlI	ACTAGT	1 cut(s)	1238
AjnI	CCWGG	3 cut(s)	448, 962, 991
AjuI	GAANNNNNNNTTGG	2 cut(s)	72, 104
AloI	GAACNNNNNNTCC	2 cut(s)	137, 169
AluBI	AGCT	5 cut(s)	360, 606, 789, 932, 1325
AluI	AGCT	5 cut(s)	360, 606, 789, 932, 1325
Alw21I	GWGCWC	1 cut(s)	791
Alw26I	GTCTC	2 cut(s)	1229, 1247
AlwI	GGATC	7 cut(s)	47, 275, 448, 461, 588, 649, 875
Aor51HI	AGCGCT	1 cut(s)	499
AoxI	GGCC	4 cut(s)	321, 428, 1088, 1151
ApeKI	GCWGC	5 cut(s)	477, 903, 971, 1074, 1322
ApoI	RAATTY	2 cut(s)	33, 742
AspA2I	CCTAGG	1 cut(s)	1148
AspLEI	GCGC	1 cut(s)	500
AspS9I	GGNCC	3 cut(s)	269, 428, 1088
AsuHPI	GGTGA	4 cut(s)	332, 590, 647, 822
AsuII	TTCGAA	1 cut(s)	179
AvaII	GGWCC	1 cut(s)	269
AvrII	CCTAGG	1 cut(s)	1148
BalI	TGGCCA	1 cut(s)	323
BamHI	GGATCC	1 cut(s)	453
BanI	GGYRCC	1 cut(s)	995
BanII	GRGCYC	1 cut(s)	791
BauI	CACGAG	1 cut(s)	481
Bbv12I	GWGCWC	1 cut(s)	791
BbvI	GCAGC	4 cut(s)	489, 915, 958, 1086
BccI	CCATC	4 cut(s)	155, 280, 351, 355
BceAI	ACGGC	1 cut(s)	559
BciT130I	CCWGG	3 cut(s)	450, 964, 993
BcoDI	GTCTC	2 cut(s)	1229, 1247
BcuI	ACTAGT	1 cut(s)	1238
BfaI	CTAG	8 cut(s)	102, 306, 522, 692, 762, 1047, 1149, 1239
BfmI	CTRYAG	5 cut(s)	462, 493, 528, 607, 1161
BfoI	RGCGCY	1 cut(s)	501
BglII	AGATCT	2 cut(s)	524, 1043
BisI	GCNGC	5 cut(s)	478, 904, 972, 1075, 1323
BlnI	CCTAGG	1 cut(s)	1148
BlsI	GCNGC	5 cut(s)	479, 905, 973, 1076, 1324
BmcAI	AGTACT	1 cut(s)	310
Bme1390I	CCNGG	3 cut(s)	450, 964, 993
Bme18I	GGWCC	1 cut(s)	269
BmgT120I	GGNCC	3 cut(s)	269, 428, 1088
BmiI	GGNNCC	5 cut(s)	65, 188, 455, 815, 997
BmrFI	CCNGG	3 cut(s)	450, 964, 993
BmsI	GCATC	3 cut(s)	35, 250, 1273
Bpu14I	TTCGAA	1 cut(s)	179
BpuEI	CTTGAG	2 cut(s)	241, 1078
BsaBI	GATNNNNATC	1 cut(s)	1218
BsaJI	CCNNGG	4 cut(s)	379, 449, 575, 1148
BsaWI	WCCGGW	1 cut(s)	256
Bsc4I	CCNNNNNNNGG	3 cut(s)	810, 839, 1109
Bse1I	ACTGG	3 cut(s)	140, 324, 1154
Bse3DI	GCAATG	1 cut(s)	424
Bse8I	GATNNNNATC	1 cut(s)	1218
BseBI	CCWGG	3 cut(s)	450, 964, 993
BseDI	CCNNGG	4 cut(s)	379, 449, 575, 1148
BseGI	GGATG	7 cut(s)	13, 265, 292, 343, 1099, 1144, 1209
BseJI	GATNNNNATC	1 cut(s)	1218
BseLI	CCNNNNNNNGG	3 cut(s)	810, 839, 1109
BseMI	GCAATG	1 cut(s)	424
BseMII	CTCAG	2 cut(s)	1119, 1254
BseNI	ACTGG	3 cut(s)	140, 324, 1154
BseRI	GAGGAG	1 cut(s)	825
BseXI	GCAGC	4 cut(s)	489, 915, 958, 1086
BseYI	CCCAGC	2 cut(s)	107, 602
BshFI	GGCC	4 cut(s)	323, 430, 1090, 1153
BshNI	GGYRCC	1 cut(s)	995
BsiHKAI	GWGCWC	1 cut(s)	791
BsiSI	CCGG	1 cut(s)	257
BslI	CCNNNNNNNGG	3 cut(s)	810, 839, 1109
BsmAI	GTCTC	2 cut(s)	1229, 1247
BsmI	GAATGC	2 cut(s)	171, 374
BsnI	GGCC	4 cut(s)	323, 430, 1090, 1153
Bsp119I	TTCGAA	1 cut(s)	179
Bsp1286I	GDGCHC	1 cut(s)	791
Bsp19I	CCATGG	1 cut(s)	379
BspACI	CCGC	2 cut(s)	113, 191
BspANI	GGCC	4 cut(s)	323, 430, 1090, 1153
BspCNI	CTCAG	2 cut(s)	1120, 1255
BspHI	TCATGA	2 cut(s)	277, 877
BspLI	GGNNCC	5 cut(s)	65, 188, 455, 815, 997
BspPI	GGATC	7 cut(s)	47, 275, 448, 461, 588, 649, 875
BspT104I	TTCGAA	1 cut(s)	179
BspT107I	GGYRCC	1 cut(s)	995
BsrDI	GCAATG	1 cut(s)	424
BsrI	ACTGG	3 cut(s)	140, 324, 1154
BssECI	CCNNGG	4 cut(s)	379, 449, 575, 1148
BssNAI	GTATAC	1 cut(s)	940
BssSI	CACGAG	1 cut(s)	481
BssT1I	CCWWGG	2 cut(s)	379, 1148
Bst1107I	GTATAC	1 cut(s)	940
Bst2BI	CACGAG	1 cut(s)	481
Bst2UI	CCWGG	3 cut(s)	450, 964, 993
Bst4CI	ACNGT	4 cut(s)	230, 368, 1012, 1165
Bst6I	CTCTTC	2 cut(s)	418, 1227
BstBI	TTCGAA	1 cut(s)	179
BstDEI	CTNAG	2 cut(s)	1128, 1263
BstDSI	CCRYGG	2 cut(s)	379, 575
BstF5I	GGATG	7 cut(s)	13, 265, 292, 343, 1099, 1144, 1209
BstH2I	RGCGCY	1 cut(s)	501
BstHHI	GCGC	1 cut(s)	500
BstMAI	GTCTC	2 cut(s)	1229, 1247
BstMWI	GCNNNNNNNGC	2 cut(s)	436, 483
BstNI	CCWGG	3 cut(s)	450, 964, 993
BstNSI	RCATGY	2 cut(s)	574, 1310
BstSCI	CCNGG	3 cut(s)	448, 962, 991
BstSFI	CTRYAG	5 cut(s)	462, 493, 528, 607, 1161
BstV1I	GCAGC	4 cut(s)	489, 915, 958, 1086
BstX2I	RGATCY	4 cut(s)	453, 524, 641, 1043
BstYI	RGATCY	4 cut(s)	453, 524, 641, 1043
BstZ17I	GTATAC	1 cut(s)	940
BsuRI	GGCC	4 cut(s)	323, 430, 1090, 1153
BtgI	CCRYGG	2 cut(s)	379, 575
BtsCI	GGATG	7 cut(s)	13, 265, 292, 343, 1099, 1144, 1209
BtsIMutI	CAGTG	4 cut(s)	147, 235, 331, 1161
CciI	TCATGA	2 cut(s)	277, 877
CfoI	GCGC	1 cut(s)	500
Cfr13I	GGNCC	3 cut(s)	269, 428, 1088
Csp6I	GTAC	2 cut(s)	309, 765
CviQI	GTAC	2 cut(s)	309, 765
DdeI	CTNAG	2 cut(s)	1128, 1263
DraIII	CACNNNGTG	1 cut(s)	302
DrdI	GACNNNNNNGTC	1 cut(s)	706
DseDI	GACNNNNNNGTC	1 cut(s)	706
EaeI	YGGCCR	1 cut(s)	321
Eam1104I	CTCTTC	2 cut(s)	418, 1227
EarI	CTCTTC	2 cut(s)	418, 1227
Ecl136II	GAGCTC	1 cut(s)	789
Eco130I	CCWWGG	2 cut(s)	379, 1148
Eco24I	GRGCYC	1 cut(s)	791
Eco32I	GATATC	1 cut(s)	1220
Eco47I	GGWCC	1 cut(s)	269
Eco47III	AGCGCT	1 cut(s)	499
Eco53kI	GAGCTC	1 cut(s)	789
Eco57I	CTGAAG	2 cut(s)	840, 1251
EcoICRI	GAGCTC	1 cut(s)	789
EcoRII	CCWGG	3 cut(s)	448, 962, 991
EcoRV	GATATC	1 cut(s)	1220
EcoT14I	CCWWGG	2 cut(s)	379, 1148
EcoT22I	ATGCAT	1 cut(s)	171
EcoT38I	GRGCYC	1 cut(s)	791
ErhI	CCWWGG	2 cut(s)	379, 1148
FauI	CCCGC	2 cut(s)	106, 198
FauNDI	CATATG	2 cut(s)	69, 205
FblI	GTMKAC	1 cut(s)	939
Fnu4HI	GCNGC	5 cut(s)	478, 904, 972, 1075, 1323
FokI	GGATG	6 cut(s)	272, 279, 330, 1086, 1131, 1216
FriOI	GRGCYC	1 cut(s)	791
Fsp4HI	GCNGC	5 cut(s)	478, 904, 972, 1075, 1323
FspBI	CTAG	8 cut(s)	102, 306, 522, 692, 762, 1047, 1149, 1239
GlaI	GCGC	1 cut(s)	499
GluI	GCNGC	5 cut(s)	478, 904, 972, 1075, 1323
GsaI	CCCAGC	2 cut(s)	111, 606
HaeII	RGCGCY	1 cut(s)	501
HaeIII	GGCC	4 cut(s)	323, 430, 1090, 1153
HapII	CCGG	1 cut(s)	257
HhaI	GCGC	1 cut(s)	500
Hin6I	GCGC	1 cut(s)	498
HinP1I	GCGC	1 cut(s)	498
HincII	GTYRAC	1 cut(s)	754
HindII	GTYRAC	1 cut(s)	754
HinfI	GANTC	2 cut(s)	595, 683
HpaII	CCGG	1 cut(s)	257
HphI	GGTGA	4 cut(s)	332, 590, 647, 822
Hpy166II	GTNNAC	3 cut(s)	553, 754, 940
Hpy188I	TCNGA	8 cut(s)	57, 514, 670, 786, 859, 1129, 1231, 1264
Hpy188III	TCNNGA	4 cut(s)	278, 522, 878, 1103
Hpy8I	GTNNAC	3 cut(s)	553, 754, 940
Hpy99I	CGWCG	1 cut(s)	372
HpyAV	CCTTC	5 cut(s)	186, 468, 837, 864, 1103
HpyCH4III	ACNGT	4 cut(s)	230, 368, 1012, 1165
HpyCH4V	TGCA	5 cut(s)	169, 740, 1117, 1286, 1322
HpyF10VI	GCNNNNNNNGC	2 cut(s)	436, 483
HpyF3I	CTNAG	2 cut(s)	1128, 1263
HspAI	GCGC	1 cut(s)	498
LmnI	GCTCC	2 cut(s)	794, 1330
Lsp1109I	GCAGC	4 cut(s)	489, 915, 958, 1086
LweI	GCATC	3 cut(s)	35, 250, 1273
MaeI	CTAG	8 cut(s)	102, 306, 522, 692, 762, 1047, 1149, 1239
MaeIII	GTNAC	4 cut(s)	1028, 1093, 1133, 1175
MboII	GAAGA	4 cut(s)	342, 435, 1059, 1244
MflI	RGATCY	4 cut(s)	453, 524, 641, 1043
MhlI	GDGCHC	1 cut(s)	791
MlsI	TGGCCA	1 cut(s)	323
MluCI	AATT	5 cut(s)	33, 58, 181, 533, 742
MluNI	TGGCCA	1 cut(s)	323
MlyI	GAGTC	1 cut(s)	692
MmeI	TCCRAC	2 cut(s)	711, 983
Mox20I	TGGCCA	1 cut(s)	323
Mph1103I	ATGCAT	1 cut(s)	171
MscI	TGGCCA	1 cut(s)	323
MseI	TTAA	1 cut(s)	915
MslI	CAYNNNNRTG	2 cut(s)	590, 1104
Msp20I	TGGCCA	1 cut(s)	323
MspI	CCGG	1 cut(s)	257
MspR9I	CCNGG	3 cut(s)	450, 964, 993
Mva1269I	GAATGC	2 cut(s)	171, 374
MvaI	CCWGG	3 cut(s)	450, 964, 993
MwoI	GCNNNNNNNGC	2 cut(s)	436, 483
NcoI	CCATGG	1 cut(s)	379
NdeI	CATATG	2 cut(s)	69, 205
NlaIV	GGNNCC	5 cut(s)	65, 188, 455, 815, 997
NmuCI	GTSAC	1 cut(s)	1093
NsiI	ATGCAT	1 cut(s)	171
NspI	RCATGY	2 cut(s)	574, 1310
NspV	TTCGAA	1 cut(s)	179
PagI	TCATGA	2 cut(s)	277, 877
PciI	ACATGT	1 cut(s)	1306
PctI	GAATGC	2 cut(s)	171, 374
PfeI	GAWTC	1 cut(s)	595
PkrI	GCNGC	5 cut(s)	479, 905, 973, 1076, 1324
PleI	GAGTC	1 cut(s)	691
PpsI	GAGTC	1 cut(s)	691
PscI	ACATGT	1 cut(s)	1306
Psp124BI	GAGCTC	1 cut(s)	791
Psp6I	CCWGG	3 cut(s)	448, 962, 991
PspFI	CCCAGC	2 cut(s)	107, 602
PspGI	CCWGG	3 cut(s)	448, 962, 991
PspN4I	GGNNCC	5 cut(s)	65, 188, 455, 815, 997
PspPI	GGNCC	3 cut(s)	269, 428, 1088
PsrI	GAACNNNNNNTAC	2 cut(s)	1005, 1037
PsuI	RGATCY	4 cut(s)	453, 524, 641, 1043
RsaI	GTAC	2 cut(s)	310, 766
RsaNI	GTAC	2 cut(s)	309, 765
RseI	CAYNNNNRTG	2 cut(s)	590, 1104
SacI	GAGCTC	1 cut(s)	791
SaqAI	TTAA	1 cut(s)	915
SatI	GCNGC	5 cut(s)	478, 904, 972, 1075, 1323
Sau96I	GGNCC	3 cut(s)	269, 428, 1088
ScaI	AGTACT	1 cut(s)	310
SchI	GAGTC	1 cut(s)	692
ScrFI	CCNGG	3 cut(s)	450, 964, 993
SduI	GDGCHC	1 cut(s)	791
SfaNI	GCATC	3 cut(s)	35, 250, 1273
SfcI	CTRYAG	5 cut(s)	462, 493, 528, 607, 1161
SfuI	TTCGAA	1 cut(s)	179
SinI	GGWCC	1 cut(s)	269
SmiMI	CAYNNNNRTG	2 cut(s)	590, 1104
SmlI	CTYRAG	2 cut(s)	220, 1057
SmoI	CTYRAG	2 cut(s)	220, 1057
SpeI	ACTAGT	1 cut(s)	1238
Sse9I	AATT	5 cut(s)	33, 58, 181, 533, 742
SsiI	CCGC	2 cut(s)	113, 191
SspMI	CTAG	8 cut(s)	102, 306, 522, 692, 762, 1047, 1149, 1239
SstI	GAGCTC	1 cut(s)	791
StyD4I	CCNGG	3 cut(s)	448, 962, 991
StyI	CCWWGG	2 cut(s)	379, 1148
TaaI	ACNGT	4 cut(s)	230, 368, 1012, 1165
TaqI	TCGA	4 cut(s)	179, 370, 710, 1222
TaqII	GACCGA	1 cut(s)	654
TasI	AATT	5 cut(s)	33, 58, 181, 533, 742
TatI	WGTACW	1 cut(s)	308
TfiI	GAWTC	1 cut(s)	595
Tru1I	TTAA	1 cut(s)	915
Tru9I	TTAA	1 cut(s)	915
TscAI	CASTG	4 cut(s)	147, 235, 331, 1161
TseFI	GTSAC	1 cut(s)	1093
TseI	GCWGC	5 cut(s)	477, 903, 971, 1074, 1322
Tsp45I	GTSAC	1 cut(s)	1093
TspDTI	ATGAA	5 cut(s)	86, 498, 943, 1122, 1248
TspGWI	ACGGA	1 cut(s)	6
TspRI	CASTG	4 cut(s)	147, 235, 331, 1161
VpaK11BI	GGWCC	1 cut(s)	269
XapI	RAATTY	2 cut(s)	33, 742
XbaI	TCTAGA	1 cut(s)	521
XceI	RCATGY	2 cut(s)	574, 1310
XcmI	CCANNNNNNNNNTGG	1 cut(s)	573
XmaJI	CCTAGG	1 cut(s)	1148
XmiI	GTMKAC	1 cut(s)	939
XspI	CTAG	8 cut(s)	102, 306, 522, 692, 762, 1047, 1149, 1239
ZrmI	AGTACT	1 cut(s)	310
Zsp2I	ATGCAT	1 cut(s)	171

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.