AT2G39435

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006066
Physical Location & Seq
Reverse (-)
16464414 .. 16467033
2620 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G39435.5

Sequence Viewer

Length: 1395 bp
ATGGAGTCTAAGGAAATTAGATCCTCCTCTGTCTTTGAGAGATCGATGCGTTTTAATGGACTCTTTCAAGATAAGCTGAGAGTTGTTGAGAAACAGAATCAACCACCAAAACGGTTTAACCAAAAGGAGTCTAATTCTGGTTTCACAGTGATTCCAACGAAGCAACTTTGTCGAGTTTATCTCCAGAAACCGAATTTGTTGGCTTCCAAGGGTATAATTGATGCACTAGCTAAGCCTCCTCATAGGATTTTTGGTAGGAGATATGATCAGCAAAACTTCAAGTCAAAGCTGAGACATCTTCACACAACAAGTATTGATCCTCTGAAACACAAATTTGGCAAGGAAGAGAATTCTCTGAGATATTTCACTCCTCTTGGTTTCAGTAGTGGTTCTCGTTTAGGAGATAGGAAGAAAGGGAAAGACCTTTCCTTTCAGGAGGAGAAACCAAAGGAAATGAAGAAACTTTGTGATCAGAAGAAGGAGAGAGTTGTGAGAGAAGTTAAATCCAGTTTGGTGGTTAGTACTTCTAGGGACAGAGTTGCAGCAGATGTGAAGTTCAGGGATTTATCTTCTTCTGGTTTCATTTCCAAGGATATAAATGCTGAGTCAGTAGATTGCTCCCATGGATCTCCGGAATGTCAGACGAATTCGGAAGATGCTCATCAACCTAGTCCTGTTTCTGTTCTGGAGCCAATGTTTTATGAAGATAACTTAGACGATAGTGAAGATATTTTAGACGACAGTGAAGATCTTCCTTATCCCAATTTTCTAAGTCTAGAGAATCAGCTGGAAACCCTGAAATCTGAATCTGAGAGTTATTCAGATGGGAGTGGGATGGAAGTATCAAGCGATGAGGAGTCTGCACTTGATTCTGCAATCAAAGAAAGCAAGGAAAGTGAACCAATTGGGTTCTTGGATACCCAAGAAAGCAGGGATTCTTCTTACATTGATGATATCTTGGCTGAAGTTTTACTTGGGGACAAGAATTGCGTTCCTGGCAAACGGGATTTAGTCATCACCCCCAAGATCTTCGAGAAACTGGAGAAGAAGTATTACACAGAGACATCTTGGAAAAGATCTGACAGAAAGATTTTGTTTGACAGAGTCAATTCAAGTCTTGTAGAGATTCTAGAATCCTTTTCAGCTACACCAACATGGAAGAAACCCGTTTCTAGGAGGCTAGGCACTGCGCTAAGCACATGTGGACTGAAACAAGAACTCTGGAAAGTACTTTCTAGGCAAGAGAAGCGATCCAAGAAAAAATCATTGGCCAAAGTGCCAGTCATAGACATTGATGAGTGGTTAGAACTCGAAGCTGATGATGAGTCAGTGGTTTGTGAATTAGAAAGTATGATAGTGGATGAGTTGTTGTCTGAGGTTGTCAGCTTCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

464

Amino Acids

52.91

Weight (kDa)

5.54

Isoelectric Point (pI)

48.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4378 PF14309 313 - 459 9.2e-25 Domain of unknown function (DUF4378)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 631
AclWI GGATC 4 cut(s) 15, 311, 634, 1245
AcoI YGGCCR 1 cut(s) 1269
AcsI RAATTY 4 cut(s) 193, 332, 349, 646
AcuI CTGAAG 1 cut(s) 984
AfaI GTAC 2 cut(s) 523, 1230
AfiI CCNNNNNNNGG 1 cut(s) 1173
AflIII ACRYGT 1 cut(s) 1199
AgsI TTSAA 3 cut(s) 68, 280, 1113
AjnI CCWGG 1 cut(s) 994
AjuI GAANNNNNNNTTGG 4 cut(s) 957, 989, 1250, 1282
AluBI AGCT 7 cut(s) 76, 230, 289, 787, 1145, 1316, 1386
AluI AGCT 7 cut(s) 76, 230, 289, 787, 1145, 1316, 1386
Alw26I GTCTC 2 cut(s) 286, 1055
AlwI GGATC 4 cut(s) 15, 311, 634, 1245
Aor13HI TCCGGA 1 cut(s) 631
AoxI GGCC 1 cut(s) 1269
ApeKI GCWGC 1 cut(s) 542
ApoI RAATTY 4 cut(s) 193, 332, 349, 646
ArsI GACNNNNNNTTYG 2 cut(s) 1266, 1298
Asp700I GAANNNNTTC 1 cut(s) 750
AspLEI GCGC 1 cut(s) 1192
AsuHPI GGTGA 1 cut(s) 1009
BalI TGGCCA 1 cut(s) 1271
BarI GAAGNNNNNNTAC 2 cut(s) 1037, 1069
BbvI GCAGC 1 cut(s) 554
BccI CCATC 2 cut(s) 818, 829
BcgI CGANNNNNNTGC 2 cut(s) 152, 186
BciT130I CCWGG 1 cut(s) 996
BciVI GTATCC 1 cut(s) 910
BclI TGATCA 2 cut(s) 265, 469
BcoDI GTCTC 2 cut(s) 286, 1055
BfaI CTAG 8 cut(s) 227, 528, 669, 776, 1130, 1173, 1181, 1236
BfuI GTATCC 1 cut(s) 910
BglII AGATCT 3 cut(s) 748, 1026, 1076
BisI GCNGC 1 cut(s) 543
BlpI GCTNAGC 2 cut(s) 231, 1193
BlsI GCNGC 1 cut(s) 544
BmcAI AGTACT 2 cut(s) 523, 1230
Bme1390I CCNGG 1 cut(s) 996
BmiI GGNNCC 1 cut(s) 690
BmrFI CCNGG 1 cut(s) 996
BmsI GCATC 3 cut(s) 36, 211, 646
BplI GAGNNNNNCTC 2 cut(s) 165, 197
BpmI CTGGAG 3 cut(s) 167, 707, 1061
Bpu1102I GCTNAGC 2 cut(s) 231, 1193
Bsa29I ATCGAT 1 cut(s) 44
BsaBI GATNNNNATC 1 cut(s) 660
BsaJI CCNNGG 3 cut(s) 207, 588, 622
BsaWI WCCGGW 1 cut(s) 631
BsaXI ACNNNNNCTCC 4 cut(s) 680, 710, 1034, 1064
Bsc4I CCNNNNNNNGG 1 cut(s) 1173
Bse1I ACTGG 3 cut(s) 507, 1044, 1280
Bse8I GATNNNNATC 1 cut(s) 660
BseAI TCCGGA 1 cut(s) 631
BseBI CCWGG 1 cut(s) 996
BseCI ATCGAT 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 207, 588, 622
BseGI GGATG 2 cut(s) 840, 1366
BseJI GATNNNNATC 1 cut(s) 660
BseLI CCNNNNNNNGG 1 cut(s) 1173
BseMII CTCAG 6 cut(s) 68, 281, 347, 594, 801, 1365
BseNI ACTGG 3 cut(s) 507, 1044, 1280
BseRI GAGGAG 5 cut(s) 16, 228, 360, 452, 869
BseXI GCAGC 1 cut(s) 554
BsgI GTGCAG 1 cut(s) 846
BshFI GGCC 1 cut(s) 1271
BshVI ATCGAT 1 cut(s) 44
BsiSI CCGG 1 cut(s) 632
BslFI GGGAC 2 cut(s) 545, 992
BslI CCNNNNNNNGG 1 cut(s) 1173
BsmAI GTCTC 2 cut(s) 286, 1055
BsmFI GGGAC 2 cut(s) 545, 992
BsnI GGCC 1 cut(s) 1271
Bsp13I TCCGGA 1 cut(s) 631
Bsp1720I GCTNAGC 2 cut(s) 231, 1193
Bsp19I CCATGG 1 cut(s) 622
BspANI GGCC 1 cut(s) 1271
BspCNI CTCAG 6 cut(s) 69, 282, 348, 595, 802, 1366
BspDI ATCGAT 1 cut(s) 44
BspEI TCCGGA 1 cut(s) 631
BspLI GGNNCC 1 cut(s) 690
BspPI GGATC 4 cut(s) 15, 311, 634, 1245
BsrI ACTGG 3 cut(s) 507, 1044, 1280
BssECI CCNNGG 3 cut(s) 207, 588, 622
BssT1I CCWWGG 3 cut(s) 207, 588, 622
Bst2UI CCWGG 1 cut(s) 996
Bst4CI ACNGT 3 cut(s) 114, 148, 743
Bst6I CTCTTC 1 cut(s) 339
BstDSI CCRYGG 1 cut(s) 622
BstF5I GGATG 2 cut(s) 840, 1366
BstHHI GCGC 1 cut(s) 1192
BstMAI GTCTC 2 cut(s) 286, 1055
BstMWI GCNNNNNNNGC 2 cut(s) 996, 1246
BstNI CCWGG 1 cut(s) 996
BstNSI RCATGY 1 cut(s) 1203
BstSCI CCNGG 1 cut(s) 994
BstV1I GCAGC 1 cut(s) 554
BstX2I RGATCY 5 cut(s) 20, 626, 748, 1026, 1076
BstXI CCANNNNNNTGG 1 cut(s) 514
BstYI RGATCY 5 cut(s) 20, 626, 748, 1026, 1076
Bsu15I ATCGAT 1 cut(s) 44
BsuI GTATCC 1 cut(s) 910
BsuRI GGCC 1 cut(s) 1271
BsuTUI ATCGAT 1 cut(s) 44
BtgI CCRYGG 1 cut(s) 622
BtgZI GCGATG 1 cut(s) 864
BtsCI GGATG 2 cut(s) 840, 1366
BtsI GCAGTG 1 cut(s) 1185
BtsIMutI CAGTG 4 cut(s) 153, 748, 1185, 1335
CfoI GCGC 1 cut(s) 1192
ClaI ATCGAT 1 cut(s) 44
Csp6I GTAC 2 cut(s) 522, 1229
CviAII CATG 4 cut(s) 623, 1155, 1200, 1390
CviQI GTAC 2 cut(s) 522, 1229
EaeI YGGCCR 1 cut(s) 1269
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Eco130I CCWWGG 3 cut(s) 207, 588, 622
Eco32I GATATC 1 cut(s) 955
Eco57I CTGAAG 1 cut(s) 984
EcoRI GAATTC 2 cut(s) 349, 646
EcoRII CCWGG 1 cut(s) 994
EcoRV GATATC 1 cut(s) 955
EcoT14I CCWWGG 3 cut(s) 207, 588, 622
ErhI CCWWGG 3 cut(s) 207, 588, 622
FaeI CATG 4 cut(s) 626, 1158, 1203, 1393
FalI AAGNNNNNCTT 2 cut(s) 957, 989
FaqI GGGAC 2 cut(s) 545, 992
FatI CATG 4 cut(s) 622, 1154, 1199, 1389
FbaI TGATCA 2 cut(s) 265, 469
Fnu4HI GCNGC 1 cut(s) 543
FokI GGATG 2 cut(s) 847, 1373
Fsp4HI GCNGC 1 cut(s) 543
FspBI CTAG 8 cut(s) 227, 528, 669, 776, 1130, 1173, 1181, 1236
GlaI GCGC 1 cut(s) 1191
GluI GCNGC 1 cut(s) 543
GsuI CTGGAG 3 cut(s) 167, 707, 1061
HaeIII GGCC 1 cut(s) 1271
HapII CCGG 1 cut(s) 632
HhaI GCGC 1 cut(s) 1192
Hin1II CATG 4 cut(s) 626, 1158, 1203, 1393
Hin6I GCGC 1 cut(s) 1190
HinP1I GCGC 1 cut(s) 1190
HpaII CCGG 1 cut(s) 632
HphI GGTGA 1 cut(s) 1009
Hpy166II GTNNAC 2 cut(s) 899, 1205
Hpy188III TCNNGA 9 cut(s) 68, 184, 434, 632, 686, 776, 1033, 1130, 1222
Hpy8I GTNNAC 2 cut(s) 899, 1205
HpyAV CCTTC 1 cut(s) 472
HpyCH4III ACNGT 3 cut(s) 114, 148, 743
HpyCH4V TGCA 4 cut(s) 224, 542, 863, 875
HpyF10VI GCNNNNNNNGC 2 cut(s) 996, 1246
Hsp92II CATG 4 cut(s) 626, 1158, 1203, 1393
HspAI GCGC 1 cut(s) 1190
Kpn2I TCCGGA 1 cut(s) 631
Ksp22I TGATCA 2 cut(s) 265, 469
LmnI GCTCC 2 cut(s) 623, 688
Lsp1109I GCAGC 1 cut(s) 554
LweI GCATC 3 cut(s) 36, 211, 646
MaeI CTAG 8 cut(s) 227, 528, 669, 776, 1130, 1173, 1181, 1236
MfeI CAATTG 1 cut(s) 903
MflI RGATCY 5 cut(s) 20, 626, 748, 1026, 1076
MlsI TGGCCA 1 cut(s) 1271
MluNI TGGCCA 1 cut(s) 1271
MlyI GAGTC 7 cut(s) 14, 54, 137, 614, 866, 1113, 1334
MmeI TCCRAC 1 cut(s) 179
Mox20I TGGCCA 1 cut(s) 1271
MroI TCCGGA 1 cut(s) 631
MroXI GAANNNNTTC 1 cut(s) 750
MscI TGGCCA 1 cut(s) 1271
MseI TTAA 3 cut(s) 54, 117, 501
MslI CAYNNNNRTG 1 cut(s) 1153
Msp20I TGGCCA 1 cut(s) 1271
MspA1I CMGCKG 1 cut(s) 787
MspI CCGG 1 cut(s) 632
MspR9I CCNGG 1 cut(s) 996
MunI CAATTG 1 cut(s) 903
MvaI CCWGG 1 cut(s) 996
MwoI GCNNNNNNNGC 2 cut(s) 996, 1246
NcoI CCATGG 1 cut(s) 622
NlaIII CATG 4 cut(s) 626, 1158, 1203, 1393
NlaIV GGNNCC 1 cut(s) 690
NspI RCATGY 1 cut(s) 1203
PciI ACATGT 1 cut(s) 1199
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 8 cut(s) 97, 151, 781, 806, 869, 935, 1126, 1133
PflFI GACNNNGTC 1 cut(s) 1103
PkrI GCNGC 1 cut(s) 544
PleI GAGTC 7 cut(s) 13, 54, 136, 613, 865, 1112, 1333
PpsI GAGTC 7 cut(s) 13, 54, 136, 613, 865, 1112, 1333
PscI ACATGT 1 cut(s) 1199
Psp6I CCWGG 1 cut(s) 994
PspGI CCWGG 1 cut(s) 994
PspN4I GGNNCC 1 cut(s) 690
PsuI RGATCY 5 cut(s) 20, 626, 748, 1026, 1076
PsyI GACNNNGTC 1 cut(s) 1103
PvuII CAGCTG 1 cut(s) 787
RsaI GTAC 2 cut(s) 523, 1230
RsaNI GTAC 2 cut(s) 522, 1229
RseI CAYNNNNRTG 1 cut(s) 1153
SaqAI TTAA 3 cut(s) 54, 117, 501
SatI GCNGC 1 cut(s) 543
ScaI AGTACT 2 cut(s) 523, 1230
SchI GAGTC 7 cut(s) 14, 54, 137, 614, 866, 1113, 1334
ScrFI CCNGG 1 cut(s) 996
SfaNI GCATC 3 cut(s) 36, 211, 646
SmiMI CAYNNNNRTG 1 cut(s) 1153
SspMI CTAG 8 cut(s) 227, 528, 669, 776, 1130, 1173, 1181, 1236
StyD4I CCNGG 1 cut(s) 994
StyI CCWWGG 3 cut(s) 207, 588, 622
TaaI ACNGT 3 cut(s) 114, 148, 743
TaqI TCGA 4 cut(s) 44, 172, 1032, 1311
TatI WGTACW 2 cut(s) 521, 1228
TfiI GAWTC 8 cut(s) 97, 151, 781, 806, 869, 935, 1126, 1133
Tru1I TTAA 3 cut(s) 54, 117, 501
Tru9I TTAA 3 cut(s) 54, 117, 501
TscAI CASTG 4 cut(s) 153, 748, 1192, 1335
TseI GCWGC 1 cut(s) 542
TspDTI ATGAA 4 cut(s) 470, 571, 717, 1378
TspRI CASTG 4 cut(s) 153, 748, 1192, 1335
Tth111I GACNNNGTC 1 cut(s) 1103
XapI RAATTY 4 cut(s) 193, 332, 349, 646
XbaI TCTAGA 2 cut(s) 775, 1129
XceI RCATGY 1 cut(s) 1203
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 8 cut(s) 227, 528, 669, 776, 1130, 1173, 1181, 1236
ZrmI AGTACT 2 cut(s) 523, 1230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.