Rorug01G0322000

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006066
Physical Location & Seq
Reverse (-)
44124536 .. 44125594
1059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0322000.1

Sequence Viewer

Length: 738 bp
ATGGAGTTGCTCGGCAACACGGACTACGCTCCTGTGGTTGATTTCGGGACCCAAACGATGTCGTCTGACTCGTTGATGACGATGATGATGCCACCTCGGCGGCAGCAGCATCAAACACAAGCGCTTCCGTCACCGTCGCCGGTGTCGTCTACAGTGCCGGAGAGTGGGACTAACTTTGAGGCCTTGAACTCCAGCGCCAATACTGCTCCTACAATTTCACAATCTGATTATTGCGTTTGTGCGATTGATTCTCTTCTTTTCGTATTCAATTTTTCCTTTCCTCGTCATCCTGAGGTGAAGTGGGCCCAGAGGGAGGACAAGGTCTTCCTTACCGTGCTTCTGCCCGATGCCAAGGACGCCAAGGTTAAGCTTGAGCCTGAAGGTGTTTTCATCTTCTCTGCTAGTGCCGGCGCCGGGAATAACCACTATGAGCTGAAACTCGACCTCTTCGATAAGGTTGATGTAGAGGAAAGCAAAATAAGTATTGGAGTGAGGAGTATAGTCTGTATTGTGGAGAAGGCAGAGAAAGTTTGGTGGAGTAAGCTATTGAAAGGAGATGGGAAGACACCGCATTATGTCAAAGTAGATTGGGACAAATGGGTTGACGAAGACGAGGATCCTTCTACGGCTCTGCCGTGTTCTTCAACGTCCGACTACTCCGGCCTTTCTGGGCTCCGTCTCTTGCTCAGCTGCACTTATAGATCAACAATCGAGAAGTTTGTCGAGTTGTTGATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4378 VARLMGL

Protein Analysis

245

Amino Acids

27.24

Weight (kDa)

4.83

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CS PF04969 97 - 173 3.5e-13 CS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 410
AccI GTMKAC 1 cut(s) 149
AciI CCGC 2 cut(s) 100, 569
AclWI GGATC 2 cut(s) 611, 624
AcuI CTGAAG 1 cut(s) 399
AcyI GRCGYC 2 cut(s) 357, 411
AfeI AGCGCT 1 cut(s) 123
AfiI CCNNNNNNNGG 3 cut(s) 164, 313, 414
AgsI TTSAA 4 cut(s) 187, 268, 550, 645
AluBI AGCT 4 cut(s) 370, 433, 544, 690
AluI AGCT 4 cut(s) 370, 433, 544, 690
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 2 cut(s) 611, 624
Aor51HI AGCGCT 1 cut(s) 123
AoxI GGCC 3 cut(s) 180, 303, 661
ApaI GGGCCC 1 cut(s) 307
ApeKI GCWGC 3 cut(s) 103, 106, 690
AspLEI GCGC 3 cut(s) 124, 197, 413
AspS9I GGNCC 3 cut(s) 48, 303, 304
AsuC2I CCSGG 1 cut(s) 415
AsuHPI GGTGA 2 cut(s) 123, 307
AvaII GGWCC 1 cut(s) 48
AxyI CCTNAGG 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 307
BamHI GGATCC 1 cut(s) 616
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 2 cut(s) 307, 675
BbsI GAAGAC 3 cut(s) 316, 569, 615
BbvI GCAGC 3 cut(s) 115, 118, 677
BccI CCATC 1 cut(s) 551
BceAI ACGGC 2 cut(s) 619, 642
BcgI CGANNNNNNTGC 2 cut(s) 70, 104
BcnI CCSGG 1 cut(s) 415
BcoDI GTCTC 1 cut(s) 683
BfaI CTAG 1 cut(s) 402
BfmI CTRYAG 1 cut(s) 150
BfoI RGCGCY 3 cut(s) 125, 198, 414
BglI GCCNNNNNGGC 1 cut(s) 97
BisI GCNGC 4 cut(s) 101, 104, 107, 691
BlpI GCTNAGC 1 cut(s) 686
BlsI GCNGC 4 cut(s) 102, 105, 108, 692
Bme1390I CCNGG 1 cut(s) 415
Bme18I GGWCC 1 cut(s) 48
BmgT120I GGNCC 3 cut(s) 48, 303, 304
BmiI GGNNCC 6 cut(s) 49, 50, 305, 412, 618, 674
BmrFI CCNGG 1 cut(s) 415
BmsI GCATC 3 cut(s) 78, 118, 337
BpiI GAAGAC 3 cut(s) 316, 569, 615
BpmI CTGGAG 1 cut(s) 175
Bpu1102I GCTNAGC 1 cut(s) 686
BpuEI CTTGAG 1 cut(s) 392
BpuMI CCSGG 1 cut(s) 415
BsaHI GRCGYC 2 cut(s) 357, 411
BsaJI CCNNGG 3 cut(s) 95, 351, 360
Bsc4I CCNNNNNNNGG 3 cut(s) 164, 313, 414
Bse118I RCCGGY 2 cut(s) 139, 407
Bse21I CCTNAGG 1 cut(s) 291
BseDI CCNNGG 3 cut(s) 95, 351, 360
BseGI GGATG 1 cut(s) 286
BseLI CCNNNNNNNGG 3 cut(s) 164, 313, 414
BseMII CTCAG 2 cut(s) 282, 700
BseRI GAGGAG 1 cut(s) 508
BseSI GKGCMC 1 cut(s) 307
BseXI GCAGC 3 cut(s) 115, 118, 677
BsgI GTGCAG 1 cut(s) 676
BshFI GGCC 3 cut(s) 182, 305, 663
BshNI GGYRCC 1 cut(s) 410
BsiSI CCGG 5 cut(s) 140, 158, 408, 414, 660
BslFI GGGAC 3 cut(s) 61, 181, 605
BslI CCNNNNNNNGG 3 cut(s) 164, 313, 414
BsmAI GTCTC 1 cut(s) 683
BsmBI CGTCTC 1 cut(s) 683
BsmFI GGGAC 3 cut(s) 61, 181, 605
BsnI GGCC 3 cut(s) 182, 305, 663
Bsp120I GGGCCC 1 cut(s) 303
Bsp1286I GDGCHC 2 cut(s) 307, 675
Bsp143I GATC 2 cut(s) 616, 701
Bsp1720I GCTNAGC 1 cut(s) 686
BspACI CCGC 2 cut(s) 100, 569
BspANI GGCC 3 cut(s) 182, 305, 663
BspCNI CTCAG 2 cut(s) 283, 699
BspLI GGNNCC 6 cut(s) 49, 50, 305, 412, 618, 674
BspPI GGATC 2 cut(s) 611, 624
BspT107I GGYRCC 1 cut(s) 410
BsrFI RCCGGY 2 cut(s) 139, 407
BssAI RCCGGY 2 cut(s) 139, 407
BssECI CCNNGG 3 cut(s) 95, 351, 360
BssMI GATC 2 cut(s) 616, 701
BssNI GRCGYC 2 cut(s) 357, 411
BssT1I CCWWGG 2 cut(s) 351, 360
Bst4CI ACNGT 3 cut(s) 135, 154, 334
Bst6I CTCTTC 2 cut(s) 258, 452
BstACI GRCGYC 2 cut(s) 357, 411
BstC8I GCNNGC 1 cut(s) 409
BstDEI CTNAG 2 cut(s) 291, 686
BstF5I GGATG 1 cut(s) 286
BstH2I RGCGCY 3 cut(s) 125, 198, 414
BstHHI GCGC 3 cut(s) 124, 197, 413
BstKTI GATC 2 cut(s) 619, 704
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 2 cut(s) 616, 701
BstMWI GCNNNNNNNGC 4 cut(s) 97, 106, 203, 356
BstSCI CCNGG 1 cut(s) 413
BstSFI CTRYAG 1 cut(s) 150
BstSLI GKGCMC 1 cut(s) 307
BstV1I GCAGC 3 cut(s) 115, 118, 677
BstV2I GAAGAC 3 cut(s) 316, 569, 615
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
Bsu36I CCTNAGG 1 cut(s) 291
BsuRI GGCC 3 cut(s) 182, 305, 663
BtsCI GGATG 1 cut(s) 286
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 1 cut(s) 409
CfoI GCGC 3 cut(s) 124, 197, 413
Cfr10I RCCGGY 2 cut(s) 139, 407
Cfr13I GGNCC 3 cut(s) 48, 303, 304
CseI GACGC 1 cut(s) 365
DdeI CTNAG 2 cut(s) 291, 686
DinI GGCGCC 1 cut(s) 412
DpnI GATC 2 cut(s) 618, 703
DpnII GATC 2 cut(s) 616, 701
Eam1104I CTCTTC 2 cut(s) 258, 452
EarI CTCTTC 2 cut(s) 258, 452
Eco130I CCWWGG 2 cut(s) 351, 360
Eco147I AGGCCT 1 cut(s) 182
Eco24I GRGCYC 2 cut(s) 307, 675
Eco47I GGWCC 1 cut(s) 48
Eco47III AGCGCT 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 399
Eco81I CCTNAGG 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 48
EcoT14I CCWWGG 2 cut(s) 351, 360
EcoT38I GRGCYC 2 cut(s) 307, 675
EgeI GGCGCC 1 cut(s) 412
EheI GGCGCC 1 cut(s) 412
ErhI CCWWGG 2 cut(s) 351, 360
Esp3I CGTCTC 1 cut(s) 683
FaiI YATR 4 cut(s) 429, 500, 576, 699
FaqI GGGAC 3 cut(s) 61, 181, 605
FblI GTMKAC 1 cut(s) 149
Fnu4HI GCNGC 4 cut(s) 101, 104, 107, 691
FokI GGATG 1 cut(s) 273
FriOI GRGCYC 2 cut(s) 307, 675
Fsp4HI GCNGC 4 cut(s) 101, 104, 107, 691
FspBI CTAG 1 cut(s) 402
GlaI GCGC 3 cut(s) 123, 196, 412
GluI GCNGC 4 cut(s) 101, 104, 107, 691
GsuI CTGGAG 1 cut(s) 175
HaeII RGCGCY 3 cut(s) 125, 198, 414
HaeIII GGCC 3 cut(s) 182, 305, 663
HapII CCGG 5 cut(s) 140, 158, 408, 414, 660
HgaI GACGC 1 cut(s) 365
HhaI GCGC 3 cut(s) 124, 197, 413
Hin1I GRCGYC 2 cut(s) 357, 411
Hin6I GCGC 3 cut(s) 122, 195, 411
HinP1I GCGC 3 cut(s) 122, 195, 411
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 368
HinfI GANTC 2 cut(s) 68, 248
HpaII CCGG 5 cut(s) 140, 158, 408, 414, 660
HphI GGTGA 2 cut(s) 123, 307
Hpy166II GTNNAC 2 cut(s) 150, 604
Hpy188I TCNGA 3 cut(s) 67, 226, 652
Hpy188III TCNNGA 3 cut(s) 46, 290, 712
Hpy8I GTNNAC 2 cut(s) 150, 604
Hpy99I CGWCG 1 cut(s) 139
HpyAV CCTTC 3 cut(s) 374, 511, 630
HpyCH4III ACNGT 3 cut(s) 135, 154, 334
HpyCH4IV ACGT 1 cut(s) 647
HpyCH4V TGCA 1 cut(s) 693
HpyF10VI GCNNNNNNNGC 4 cut(s) 97, 106, 203, 356
HpyF3I CTNAG 2 cut(s) 291, 686
HpySE526I ACGT 1 cut(s) 647
Hsp92I GRCGYC 2 cut(s) 357, 411
HspAI GCGC 3 cut(s) 122, 195, 411
KasI GGCGCC 1 cut(s) 410
KflI GGGWCCC 1 cut(s) 48
KroI GCCGGC 1 cut(s) 407
KroNI GCCGGC 1 cut(s) 409
Kzo9I GATC 2 cut(s) 616, 701
LmnI GCTCC 3 cut(s) 34, 211, 678
Lsp1109I GCAGC 3 cut(s) 115, 118, 677
LweI GCATC 3 cut(s) 78, 118, 337
MaeI CTAG 1 cut(s) 402
MaeII ACGT 1 cut(s) 647
MaeIII GTNAC 1 cut(s) 129
MalI GATC 2 cut(s) 618, 703
MboI GATC 2 cut(s) 616, 701
MboII GAAGA 7 cut(s) 245, 316, 385, 439, 574, 620, 633
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 2 cut(s) 307, 675
MluCI AATT 2 cut(s) 213, 268
Mly113I GGCGCC 1 cut(s) 411
MlyI GAGTC 1 cut(s) 62
MmeI TCCRAC 1 cut(s) 675
MroNI GCCGGC 1 cut(s) 407
MseI TTAA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 690
MspI CCGG 5 cut(s) 140, 158, 408, 414, 660
MspR9I CCNGG 1 cut(s) 415
MwoI GCNNNNNNNGC 4 cut(s) 97, 106, 203, 356
NaeI GCCGGC 1 cut(s) 409
NarI GGCGCC 1 cut(s) 411
NciI CCSGG 1 cut(s) 415
NdeII GATC 2 cut(s) 616, 701
NgoMIV GCCGGC 1 cut(s) 407
NlaIV GGNNCC 6 cut(s) 49, 50, 305, 412, 618, 674
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 1 cut(s) 129
PceI AGGCCT 1 cut(s) 182
PcsI WCGNNNNNNNCGW 5 cut(s) 68, 77, 143, 447, 632
PdiI GCCGGC 1 cut(s) 409
PfeI GAWTC 1 cut(s) 248
PflFI GACNNNGTC 1 cut(s) 320
PkrI GCNGC 4 cut(s) 102, 105, 108, 692
PleI GAGTC 1 cut(s) 62
PluTI GGCGCC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 62
PpuMI RGGWCCY 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 48
PspN4I GGNNCC 6 cut(s) 49, 50, 305, 412, 618, 674
PspOMI GGGCCC 1 cut(s) 303
PspPI GGNCC 3 cut(s) 48, 303, 304
PspPPI RGGWCCY 1 cut(s) 48
PsuI RGATCY 1 cut(s) 616
PsyI GACNNNGTC 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 690
SaqAI TTAA 1 cut(s) 366
SatI GCNGC 4 cut(s) 101, 104, 107, 691
Sau3AI GATC 2 cut(s) 616, 701
Sau96I GGNCC 3 cut(s) 48, 303, 304
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 415
SduI GDGCHC 2 cut(s) 307, 675
SfaNI GCATC 3 cut(s) 78, 118, 337
SfcI CTRYAG 1 cut(s) 150
SfoI GGCGCC 1 cut(s) 412
SgrAI CRCCGGYG 1 cut(s) 139
SinI GGWCC 1 cut(s) 48
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 2 cut(s) 213, 268
SseBI AGGCCT 1 cut(s) 182
SsiI CCGC 2 cut(s) 100, 569
SspDI GGCGCC 1 cut(s) 410
SspMI CTAG 1 cut(s) 402
StuI AGGCCT 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 413
StyI CCWWGG 2 cut(s) 351, 360
TaaI ACNGT 3 cut(s) 135, 154, 334
TaiI ACGT 1 cut(s) 650
TaqI TCGA 4 cut(s) 441, 450, 711, 723
TasI AATT 2 cut(s) 213, 268
TauI GCSGC 1 cut(s) 103
TfiI GAWTC 1 cut(s) 248
Tru1I TTAA 1 cut(s) 366
Tru9I TTAA 1 cut(s) 366
TscAI CASTG 1 cut(s) 159
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 3 cut(s) 103, 106, 690
Tsp45I GTSAC 1 cut(s) 129
TspDTI ATGAA 1 cut(s) 379
TspGWI ACGGA 3 cut(s) 35, 117, 665
TspRI CASTG 1 cut(s) 159
Tth111I GACNNNGTC 1 cut(s) 320
VpaK11BI GGWCC 1 cut(s) 48
XmiI GTMKAC 1 cut(s) 149
XspI CTAG 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.